WO2018041274A3 - Dna测序方法 - Google Patents

Dna测序方法 Download PDF

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Publication number
WO2018041274A3
WO2018041274A3 PCT/CN2017/108325 CN2017108325W WO2018041274A3 WO 2018041274 A3 WO2018041274 A3 WO 2018041274A3 CN 2017108325 W CN2017108325 W CN 2017108325W WO 2018041274 A3 WO2018041274 A3 WO 2018041274A3
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WO
WIPO (PCT)
Prior art keywords
dna
tag sequence
dna sequencing
sequenced
sequencing
Prior art date
Application number
PCT/CN2017/108325
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English (en)
French (fr)
Other versions
WO2018041274A2 (zh
Inventor
郭雪峰
王晓龙
慈海娜
Original Assignee
北京大学
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 北京大学 filed Critical 北京大学
Priority to US16/327,535 priority Critical patent/US20190330695A1/en
Priority to JP2019506126A priority patent/JP2020500002A/ja
Priority to EP17845576.2A priority patent/EP3508587A4/en
Publication of WO2018041274A2 publication Critical patent/WO2018041274A2/zh
Publication of WO2018041274A3 publication Critical patent/WO2018041274A3/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • H—ELECTRICITY
    • H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
    • H10K—ORGANIC ELECTRIC SOLID-STATE DEVICES
    • H10K85/00—Organic materials used in the body or electrodes of devices covered by this subclass
    • H10K85/40—Organosilicon compounds, e.g. TIPS pentacene
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y35/00—Methods or apparatus for measurement or analysis of nanostructures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Materials Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

本发明公开了一种DNA测序方法。本发明所提供的DNA测序方法依次包括如下步骤：（1）在待测DNA的3'末端增加标签序列，形成含有标签序列的待测DNA；所述标签序列的核苷酸序列与测序引物的核苷酸序列反向互补；（2）将所述含有标签序列的待测DNA和所述测序引物混合，形成5'末端双链主体单链形式的产物；（3）完成步骤（2）后，将产物分别与dATP、dCTP、dTTP和dGTP混合，得到四个体系，分别将每个体系加入DNA聚合酶修饰的单分子器件，读取电信号。实验证明，采用本发明提供的方法可进行DNA测序，具有重要的应用价值。
PCT/CN2017/108325 2016-08-31 2017-10-30 Dna测序方法 WO2018041274A2 (zh)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US16/327,535 US20190330695A1 (en) 2016-08-31 2017-10-30 Dna sequencing method
JP2019506126A JP2020500002A (ja) 2016-08-31 2017-10-30 Ｄｎａ塩基配列決定法
EP17845576.2A EP3508587A4 (en) 2016-08-31 2017-10-30 DNA SEQUENCING METHOD

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201610798113.XA CN106244712B (zh) 2016-08-31 2016-08-31 Dna测序方法
CN201610798113.X 2016-08-31

Publications (2)

Publication Number Publication Date
WO2018041274A2 WO2018041274A2 (zh) 2018-03-08
WO2018041274A3 true WO2018041274A3 (zh) 2018-04-26

Family

ID=58081022

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2017/108325 WO2018041274A2 (zh) 2016-08-31 2017-10-30 Dna测序方法

Country Status (5)

Country Link
US (1) US20190330695A1 (zh)
EP (1) EP3508587A4 (zh)
JP (1) JP2020500002A (zh)
CN (1) CN106244712B (zh)
WO (1) WO2018041274A2 (zh)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106244712B (zh) * 2016-08-31 2019-11-19 北京大学 Dna测序方法
BR112020023494A2 (pt) 2018-05-17 2021-03-30 Stuart Lindsay Dispositivo, sistema e método para medição elétrica direta de atividade enzimática, bem como método para sequenciamento
JP2023516167A (ja) 2020-02-28 2023-04-18 アリゾナ・ボード・オブ・リージェンツ・オン・ビハーフ・オブ・アリゾナ・ステイト・ユニバーシティー 生体高分子の配列を決定する方法
CN111474365B (zh) * 2020-03-27 2021-09-17 北京大学 一种生物传感器及制备方法和病毒检测系统及方法
CN112322715B (zh) * 2020-11-17 2022-11-25 清华大学 一种核酸测序方法
US20230059925A1 (en) * 2021-08-18 2023-02-23 The Charles Stark Draper Laboratory, Inc. Macromolecular Sequencing by Quantum Transport Through Molecular Bridges

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012005857A1 (en) * 2010-06-08 2012-01-12 President And Fellows Of Harvard College Nanopore device with graphene supported artificial lipid membrane
CN104630358A (zh) * 2015-01-30 2015-05-20 中国科学院重庆绿色智能技术研究院 Dna测序方法及其系统
CN105092647A (zh) * 2015-08-20 2015-11-25 中国科学院重庆绿色智能技术研究院 一种基于分子构象变化的测序方法
CN106244712A (zh) * 2016-08-31 2016-12-21 北京大学 Dna测序方法

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US6451191B1 (en) * 1999-11-18 2002-09-17 3M Innovative Properties Company Film based addressable programmable electronic matrix articles and methods of manufacturing and using the same
CN102317471A (zh) * 2008-12-11 2012-01-11 加利福尼亚大学董事会 用于单个dna分子的直接测序的方法和系统
CN102643893A (zh) * 2011-02-16 2012-08-22 陈国燕 基于引物阵列芯片的dna测序方法
WO2014182630A1 (en) * 2013-05-06 2014-11-13 Pacific Biosciences Of California , Inc. Real-time electronic sequencing
PL3169805T3 (pl) * 2014-07-15 2019-01-31 Illumina, Inc. Aktywowane biochemicznie urządzenie elektroniczne
CN104359946B (zh) * 2014-10-23 2017-05-31 北京大学 一种基于纳米对电极的单分子核酸测序器件
CA2971268A1 (en) * 2014-12-18 2016-06-23 The Regents Of The University Of California Detection of nucleic acid polymerase conformational changes using a nanotube

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012005857A1 (en) * 2010-06-08 2012-01-12 President And Fellows Of Harvard College Nanopore device with graphene supported artificial lipid membrane
CN104630358A (zh) * 2015-01-30 2015-05-20 中国科学院重庆绿色智能技术研究院 Dna测序方法及其系统
CN105092647A (zh) * 2015-08-20 2015-11-25 中国科学院重庆绿色智能技术研究院 一种基于分子构象变化的测序方法
CN106244712A (zh) * 2016-08-31 2016-12-21 北京大学 Dna测序方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE Protein [O] 14 May 2013 (2013-05-14), "DNA polymerase I [Escherichia coli]", XP055478373, Database accession no. WP_000250009.1 *

Also Published As

Publication number Publication date
EP3508587A2 (en) 2019-07-10
US20190330695A1 (en) 2019-10-31
CN106244712A (zh) 2016-12-21
CN106244712B (zh) 2019-11-19
WO2018041274A2 (zh) 2018-03-08
EP3508587A4 (en) 2019-10-30
JP2020500002A (ja) 2020-01-09

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