WO2017219350A1 - Systèmes et procédés de cyclage thermique - Google Patents

Systèmes et procédés de cyclage thermique Download PDF

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Publication number
WO2017219350A1
WO2017219350A1 PCT/CN2016/087046 CN2016087046W WO2017219350A1 WO 2017219350 A1 WO2017219350 A1 WO 2017219350A1 CN 2016087046 W CN2016087046 W CN 2016087046W WO 2017219350 A1 WO2017219350 A1 WO 2017219350A1
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WO
WIPO (PCT)
Prior art keywords
reaction
nucleic acid
heating
base
reaction vessel
Prior art date
Application number
PCT/CN2016/087046
Other languages
English (en)
Inventor
Xiang Li
Fei Yu
Original Assignee
Coyote Bioscience Co., Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Coyote Bioscience Co., Ltd filed Critical Coyote Bioscience Co., Ltd
Priority to PCT/CN2016/087046 priority Critical patent/WO2017219350A1/fr
Priority to CN201680088726.2A priority patent/CN109642197A/zh
Publication of WO2017219350A1 publication Critical patent/WO2017219350A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38—Temperature-responsive control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/043—Hinged closures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B01L2300/047—Additional chamber, reservoir
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/185—Means for temperature control using fluid heat transfer medium using a liquid as fluid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention porte sur des systèmes d'amplification des acides nucléiques et sur des méthodes pour effectuer des amplifications des acides nucléiques avec de tels systèmes.
PCT/CN2016/087046 2016-06-24 2016-06-24 Systèmes et procédés de cyclage thermique WO2017219350A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PCT/CN2016/087046 WO2017219350A1 (fr) 2016-06-24 2016-06-24 Systèmes et procédés de cyclage thermique
CN201680088726.2A CN109642197A (zh) 2016-06-24 2016-06-24 用于热循环的系统和方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2016/087046 WO2017219350A1 (fr) 2016-06-24 2016-06-24 Systèmes et procédés de cyclage thermique

Publications (1)

Publication Number Publication Date
WO2017219350A1 true WO2017219350A1 (fr) 2017-12-28

Family

ID=60783778

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2016/087046 WO2017219350A1 (fr) 2016-06-24 2016-06-24 Systèmes et procédés de cyclage thermique

Country Status (2)

Country Link
CN (1) CN109642197A (fr)
WO (1) WO2017219350A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6703236B2 (en) * 1990-11-29 2004-03-09 Applera Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
US20060105433A1 (en) * 2004-11-18 2006-05-18 Bickmore William D Jr Rapid thermocycler
CN101522909A (zh) * 2006-05-17 2009-09-02 加利福尼亚技术学院 热循环系统
WO2012166913A1 (fr) * 2011-06-01 2012-12-06 Streck, Inc. Système thermocycleur rapide pour une amplification rapide d'acides nucléiques et procédés s'y rapportant
CN103243017A (zh) * 2012-02-09 2013-08-14 李响 利用整体结构储热和散热的薄型pcr仪
WO2015176674A1 (fr) * 2014-05-21 2015-11-26 Coyote Bioscience Co., Ltd. Systèmes et procédés de thermocyclage

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105358673A (zh) * 2014-05-21 2016-02-24 卡尤迪生物科技（北京）有限公司 用于热循环的系统和方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6703236B2 (en) * 1990-11-29 2004-03-09 Applera Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
US20060105433A1 (en) * 2004-11-18 2006-05-18 Bickmore William D Jr Rapid thermocycler
CN101522909A (zh) * 2006-05-17 2009-09-02 加利福尼亚技术学院 热循环系统
WO2012166913A1 (fr) * 2011-06-01 2012-12-06 Streck, Inc. Système thermocycleur rapide pour une amplification rapide d'acides nucléiques et procédés s'y rapportant
CN103243017A (zh) * 2012-02-09 2013-08-14 李响 利用整体结构储热和散热的薄型pcr仪
WO2015176674A1 (fr) * 2014-05-21 2015-11-26 Coyote Bioscience Co., Ltd. Systèmes et procédés de thermocyclage

Also Published As

Publication number Publication date
CN109642197A (zh) 2019-04-16

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