WO2017173689A1 - Application using tolypocladium inflatum to manufacture shrimp feed and/or formulation for treating or preventing vibriosis - Google Patents

Application using tolypocladium inflatum to manufacture shrimp feed and/or formulation for treating or preventing vibriosis Download PDF

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Publication number
WO2017173689A1
WO2017173689A1 PCT/CN2016/080855 CN2016080855W WO2017173689A1 WO 2017173689 A1 WO2017173689 A1 WO 2017173689A1 CN 2016080855 W CN2016080855 W CN 2016080855W WO 2017173689 A1 WO2017173689 A1 WO 2017173689A1
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Prior art keywords
trichoderma
feed
preparation
cfu
shrimp
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PCT/CN2016/080855
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French (fr)
Chinese (zh)
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吴政宏
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吴政宏
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/885—Trichoderma
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Definitions

  • the present invention relates to a feed and/or preparation for administration to shrimp, and more particularly to the use of Trichoderma for the preparation of a feed and/or preparation for shrimp for the treatment or prevention of Vibrio infection.
  • Vibrio is one of the common bacterial groups in the marine environment, and it has strong adaptability and resistance to become a dominant population in the seawater environment.
  • the disease caused by Vibrio is a bacterial disease that is prevalent and extremely harmful in aquatic animals such as fish, shrimp, crab and shellfish, which can cause serious economic losses in the aquaculture industry.
  • Vibrio belongs to Gram-negative bacteria, and Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio vulnificus or Vibrio cholerae can infect shrimp.
  • Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio vulnificus or Vibrio cholerae can infect shrimp.
  • AHPNS Acute Hepatopancreatic Necrosis Syndrome
  • Vibrio parahaemolyticus produces a toxin in the hepatopancreas of shrimps after being infected by Vibrio parahaemolyticus.
  • hepatic and pancreatic lesions and functional disorders including slow growth, spiral swimming, shell damage, hepatopancreas redness, swelling, and even atrophy and necrosis; more often, hepatic pancreatic duct epithelium, pancreatic microvilli tissue of shrimp
  • the intestinal mucosa epithelium and proliferating cells form white organic matter which forms white stool in the intestinal tract.
  • the administration of antibiotics to the culture ponds is not effective after the bacteria develop resistance, and failure to comply with the withdrawal schedule will result in the antibiotics remaining in the shrimps, which will threaten the health of the people who eat the shrimps.
  • the culture water used for antibiotics is not properly disposed, it may cause drug contamination in coastal waters and inland areas. Feeding vitamin C, polysaccharides, herbal agents and other nutrients that help boost the immunity of shrimps does not work for already infected shrimps, nor does it inhibit Vibrio.
  • As for the use of chemical disinfectants only Vibrio in the culture pond can be killed, and it is impossible to produce an effective therapeutic effect on the Vibrio and the lesions caused by the shrimp.
  • the invention provides a method for solving the problems of the prior art by providing a mold for the treatment of Trichoderma Or the use of feeds and/or preparations for the prevention of Vibrio infected shrimp.
  • Trichoderma for the preparation of a feed and/or a preparation for the treatment or prevention of a Vibrio infection, the feed comprising a dry granule feed or a fermentation treatment Liquid feed.
  • an application of Trichoderma in the preparation of a feed and/or a preparation for treating shrimp for treating or preventing Vibrio infection wherein 0.01 to 200 are added per kg of the feed.
  • the gram of the Trichoderma, and the amount of the Trichoderma is 1 ⁇ 10 2 cfu / g - 5 ⁇ 10 10 cfu / g.
  • Trichoderma in the preparation of a feed and/or a preparation for treating shrimp for treating or preventing Vibrio infection, wherein 0.1 g of the feed is added per kg. Trichoderma, and the amount of the fungus of the Trichoderma is 5 ⁇ 10 8 cfu / g.
  • Trichoderma for the preparation of a feed and/or a preparation for treating shrimp for treating or preventing a Vibrio infection, wherein the amount of the Trichoderma in the preparation is It is 1 ⁇ 10 2 cfu / g to 5 ⁇ 10 10 cfu / g.
  • Trichoderma for the preparation of a feed and/or formulation for shrimp for the treatment or prevention of Vibrio infection, said formulation comprising an excipient.
  • Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection of shrimp, the excipient from soy flour, rice bran, soybean meal One or more selected from the group consisting of sesame meal, ricin seed meal, rapeseed meal, peanut meal and bran.
  • Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection, the preparation comprising Trichoderma, a carbon source, a nitrogen source and water.
  • a use of Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection, the weight ratio of said carbon source to said bacterial powder
  • the weight ratio of the nitrogen source to the bacterial powder is 5:1 to 100:1
  • the water and the bacterial powder, the carbon source and the The weight ratio of the total weight of the nitrogen source is from 5:1 to 50:1.
  • a Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection
  • the preparation comprising a mushroom powder comprising the Trichoderma , granulated sugar, rice bran and water, characterized in that the weight ratio of the granulated sugar to the bacterium powder is 20:1, the weight ratio of the rice bran to the bacterium powder is 20:1 and the The weight ratio of water to the total weight of the bacterin, the granulated sugar and the rice bran is 19.51:1
  • Trichoderma viride for the preparation of a feed and/or a preparation for the treatment or prevention of a Vibrio infection of a shrimp Trichoderma harzianum, Trichoderma hamatum, Trichoderma Longibrachiatum), Trichoderma koningii, Trichoderma asperellum, Trichoderma pseudokoningii, Trichoderma spirale, Trichoderma fertile, stout wood
  • Trichoderma strigosum Trichoderma longipile.
  • a use of a Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection the Vibrio infection being acute hepatopancreatic necrosis syndrome ( Acute Hepatopancreatic Necrosis Syndrome (AHPNS) or Vibrio infection of Hepatopancreatic Necrosis Syndrome (HPNS).
  • AHPNS Acute Hepatopancreatic Necrosis Syndrome
  • HPNS Vibrio infection of Hepatopancreatic Necrosis Syndrome
  • Trichoderma sp. for preparing a feed and/or a preparation for treating or preventing a Vibrio infection of a shrimp, the Vibrio algae, Vibrio anguillarum , Vibrio parahaemolyticus, Vibrio vulnificus or Vibrio cholerae.
  • the shrimp feed and/or preparation contains Trichoderma, and after being administered to the shrimp culture pond, the Spirulina-infected shrimp can be effectively treated or prevented, and the Shrimp causes any side effects.
  • a first embodiment of the present invention is the use of Trichoderma for the preparation of a feed for shrimp for the treatment or prevention of Vibrio infection.
  • the Trichoderma is Trichoderma viride.
  • the Trichoderma can be from Trichoderma harzianum, Trichoderma hamatum, Trichoderma longibrachiatum, Trichoderma koningii, Trichoderma oxysporum (Trichoderma asperellum), Trichoderma pseudokoningii, Trichoderma spirale, Trichoderma fertile, Trichoderma strigosum, and Trichoderma longipile One or more selected in the group.
  • the Vibrio may be Vibrio alginolyticus, Vibrio anguillarum, Vibrio parahaemolyticus, Vibrio vulnificus or Vibrio cholerae.
  • the Vibrio infection may be a Vibrio infection of Acute Hepatopancreatic Necrosis Syndrome (AHPNS) or Hepatopancreatic Necrosis Syndrome (HPNS).
  • AHPNS Acute Hepatopancreatic Necrosis Syndrome
  • HPNS Hepatopancreatic Necrosis Syndrome
  • the feed is a dry granule feed.
  • the feed may be a liquid feed.
  • the feed is fermented feed and is easily digested and absorbed.
  • the weight of Trichoderma per kg of feed may range from 0.01 g to 200 g and includes 0.01 g and 200 g, and the amount of the Trichoderma may be 1 ⁇ 10 2 cfu/g ⁇ 5 ⁇ 10 10 cfu / g and includes 1 ⁇ 10 2 cfu / g and 5 ⁇ 10 10 cfu / g.
  • the above-mentioned feed prepared to contain Trichoderma viride is administered by direct feeding to white shrimp having acute hepatopancreatic necrosis syndrome but also having feeding ability. And the liver, pancreas and gut of the shrimp were observed three days after the feeding, and the liver, the pancreas and the gut which were originally whitened and weakened by rickets were restored to normal. Dark black result.
  • a second embodiment of the present invention is the use of Trichoderma for the preparation of a shrimp for the treatment or prevention of Vibrio infection.
  • the preparation process of the Trichoderma-containing preparation of the present invention is as follows.
  • Trichoderma viride powder 5 ⁇ 10 8 cfu/g of Trichoderma viride powder was mixed with carbon source, nitrogen source and distilled water, and aerated at 5 liter/min, and the amount of Trichoderma obtained after 72 hours was 5 ⁇ 10 9 .
  • the carbon source used in the embodiment is granulated sugar and the weight is 20 times that of the Trichoderma bacterium powder; and the nitrogen source used is rice bran, and the weight is as described. 20 times of the Trichoderma powder; and the weight of the distilled water is 19.51 times the total weight of the Trichoderma powder, the sugar and the rice bran.
  • the present invention is not limited to the amount of Trichoderma bacteria of 5 ⁇ 10 9 cfu / g.
  • the amount of the fungus of Trichoderma can be from 1 ⁇ 10 2 cfu / g to 5 ⁇ 10 10 cfu / g and includes 1 ⁇ 10 2 cfu / g and 5 ⁇ 10 10 cfu / g.
  • the weight ratio of the carbon source to the bacterial powder may be 5:1 to 100:1
  • the weight ratio of the nitrogen source to the bacterial powder may be 5:
  • the weight ratio of 1 to 100:1 and the total weight of the water and the bacterial powder, the carbon source, and the nitrogen source may be 5:1 to 50:1.
  • Trichoderma can be sprayed and dried on an excipient to form a formulation having a bacterial count of from 1 x 10 2 cfu/g to 5 x 10 10 cfu/g.
  • the excipient is one or more selected from the group consisting of soy flour, rice bran, soybean meal, sesame meal, castor seed meal, rapeseed meal, peanut meal and bran.
  • the preparation directly inputs the white shrimp culture pond in which acute hepatopancreatic necrosis syndrome has occurred, and the input amount of the preparation is inversely related to the recovery time of the shrimp. For example, if the concentration of the bacterial liquid (formulation) in the culture pond is 4 ppm, the liver, the pancreas, and the gut line of the white shrimp originally whitened and weakened due to rickets may be obtained after three days. Restores to normal dark black and can be used for normal feeding. If the concentration of the bacterial solution (formulation) in the culture pond is 2 ppm, it takes at least six days to obtain an effective therapeutic effect.
  • the Trichoderma-containing feed or preparation of the present invention has the same or similar therapeutic or prophylactic effects on other shrimps.

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Abstract

An application using Tolypocladium inflatum to manufacture a shrimp feed and/or formulation for treating or preventing vibriosis. The shrimp feed is a dry, granulated feed or a fermented feed, wherein 0.01-200 g of Tolypocladium inflatum is added to every kilogram of the feed. The plate count of Tolypocladium inflatum in the feed is 1 × 102-5 × 1010 cfu/g, and the plate count of Tolypocladium inflatum in the formulation is 1 × 102-5 × 1010 cfu/g.

Description

将木霉菌予以在制备用于治疗或预防弧菌感染的虾的饲料及/或制剂的应用Application of Trichoderma in the preparation of feed and/or preparation of shrimp for treating or preventing Vibrio infection 技术领域Technical field
本发明涉及一种投予虾的饲料及/或制剂，特别是涉及一种将木霉菌予以在制备用于治疗或预防弧菌感染的虾的饲料及/或制剂的应用。The present invention relates to a feed and/or preparation for administration to shrimp, and more particularly to the use of Trichoderma for the preparation of a feed and/or preparation for shrimp for the treatment or prevention of Vibrio infection.
背景技术Background technique
弧菌是海洋环境中常见的细菌类群之一，并具有强适应性及抗逆性而成为海水环境中的优势种群。由弧菌所引起的疾病是一种在养殖鱼、虾、蟹及贝类等水产动物中普遍流行且危害极大的细菌性疾病，会导致水产养殖业严重的经济损失。Vibrio is one of the common bacterial groups in the marine environment, and it has strong adaptability and resistance to become a dominant population in the seawater environment. The disease caused by Vibrio is a bacterial disease that is prevalent and extremely harmful in aquatic animals such as fish, shrimp, crab and shellfish, which can cause serious economic losses in the aquaculture industry.
弧菌属于革兰氏阴性菌，其中的溶藻弧菌、副溶血弧菌、创伤弧菌或霍乱弧菌会感染虾类。举例而言，受副溶血弧菌感染而造成急性肝胰腺坏死综合症(Acute Hepatopancreatic Necrosis Syndrome,AHPNS)的虾子，其会因副溶血弧菌被噬菌体感染后对虾类的肝胰脏产生毒素，而使得肝胰脏出现病变及功能性障碍，包括生长缓慢、螺旋式游泳、外壳损害、肝胰脏发红、肿大、甚至萎缩坏死；更甚者，虾子的肝胰腺管上皮、胰腺微绒毛组织、肠道黏膜上皮与增生细胞形成白色有机物在肠道中形成白便。Vibrio belongs to Gram-negative bacteria, and Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio vulnificus or Vibrio cholerae can infect shrimp. For example, a shrimp infected with Acute Hepatopancreatic Necrosis Syndrome (AHPNS), which is infected by Vibrio parahaemolyticus, produces a toxin in the hepatopancreas of shrimps after being infected by Vibrio parahaemolyticus. Causes hepatic and pancreatic lesions and functional disorders, including slow growth, spiral swimming, shell damage, hepatopancreas redness, swelling, and even atrophy and necrosis; more often, hepatic pancreatic duct epithelium, pancreatic microvilli tissue of shrimp The intestinal mucosa epithelium and proliferating cells form white organic matter which forms white stool in the intestinal tract.
过去治疗或预防受弧菌感染的虾类的方式主要有投予抗生素、营养物质及化学消毒剂，然而这些做法仍有以下缺点。In the past, the treatment or prevention of shrimp infected with Vibrio was mainly carried out by administering antibiotics, nutrients and chemical disinfectants. However, these practices still have the following disadvantages.
首先，对养殖池投予抗生素会在细菌产生抗药性的后效果不彰，且若未能遵守停药期规定而导致抗生素残留在虾中，会威胁到食用所述的虾子的人们健康。此外，经投予抗生素的养殖用水若未妥善处理亦可能造成沿海水域及内陆地区的药物污染。而喂食维他命C、多醣体、中草药剂及其它有助于提升虾类的免疫力的营养物质对于已经受感染的虾类不具效用，亦无法抑制弧菌。至于采用化学消毒剂仅能杀灭养殖池中的弧菌，无法对虾子体内的弧菌及其造成的病变产生有效的治疗效果。First, the administration of antibiotics to the culture ponds is not effective after the bacteria develop resistance, and failure to comply with the withdrawal schedule will result in the antibiotics remaining in the shrimps, which will threaten the health of the people who eat the shrimps. In addition, if the culture water used for antibiotics is not properly disposed, it may cause drug contamination in coastal waters and inland areas. Feeding vitamin C, polysaccharides, herbal agents and other nutrients that help boost the immunity of shrimps does not work for already infected shrimps, nor does it inhibit Vibrio. As for the use of chemical disinfectants, only Vibrio in the culture pond can be killed, and it is impossible to produce an effective therapeutic effect on the Vibrio and the lesions caused by the shrimp.
发明内容Summary of the invention
因此，本发明的目的即在提供一种将木霉菌予以在制备用于治疗或预防弧菌感染的虾的饲料及/或制剂的应用，而能在不对虾产生副作用或伤害的前提下有效地治疗或预防受弧菌感染及其相关病变的虾。Accordingly, it is an object of the present invention to provide an application of a Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection of shrimp, which can be effectively produced without causing side effects or damage to the shrimp. Treatment or prevention of shrimp infected with Vibrio infection and its associated lesions.
本发明为解决现有技术的问题所采用的技术手段提供一种将木霉菌予以在制备用于治疗 或预防弧菌感染的虾的饲料及/或制剂的应用。The invention provides a method for solving the problems of the prior art by providing a mold for the treatment of Trichoderma Or the use of feeds and/or preparations for the prevention of Vibrio infected shrimp.
在本发明的一实施例中提供一种将木霉菌予以在制备用于治疗或预防弧菌感染的虾的饲料及/或制剂的应用，所述的饲料包括干性颗粒的饲料或经发酵处理的液态的饲料。In an embodiment of the invention there is provided a use of Trichoderma for the preparation of a feed and/or a preparation for the treatment or prevention of a Vibrio infection, the feed comprising a dry granule feed or a fermentation treatment Liquid feed.
在本发明的一实施例中提供一种将木霉菌予以在制备用于治疗或预防弧菌感染的虾的饲料及/或制剂的应用，每公斤的所述的饲料中添加有0.01克～200克的所述的木霉菌，且所述的木霉菌的菌量为1×102cfu/g～5×1010cfu/g。In an embodiment of the present invention, there is provided an application of Trichoderma in the preparation of a feed and/or a preparation for treating shrimp for treating or preventing Vibrio infection, wherein 0.01 to 200 are added per kg of the feed. The gram of the Trichoderma, and the amount of the Trichoderma is 1 × 10 2 cfu / g - 5 × 10 10 cfu / g.
在本发明的一实施例中提供一种将木霉菌予以在制备用于治疗或预防弧菌感染的虾的饲料及/或制剂的应用，每公斤的所述的饲料中添加有0.1克的所述的木霉菌，且所述的木霉菌的菌量为5×108cfu/g。In an embodiment of the present invention, there is provided an application of Trichoderma in the preparation of a feed and/or a preparation for treating shrimp for treating or preventing Vibrio infection, wherein 0.1 g of the feed is added per kg. Trichoderma, and the amount of the fungus of the Trichoderma is 5 × 10 8 cfu / g.
在本发明的一实施例中提供一种将木霉菌予以在制备用于治疗或预防弧菌感染的虾的饲料及/或制剂的应用，所述的制剂中的所述的木霉菌的菌量为1×102cfu/g～5×1010cfu/g。In an embodiment of the present invention, there is provided a use of Trichoderma for the preparation of a feed and/or a preparation for treating shrimp for treating or preventing a Vibrio infection, wherein the amount of the Trichoderma in the preparation is It is 1 × 10 2 cfu / g to 5 × 10 10 cfu / g.
在本发明的一实施例中提供一种将木霉菌予以在制备用于治疗或预防弧菌感染的虾的饲料及/或制剂的应用，所述的制剂包括赋形剂。In one embodiment of the invention, there is provided the use of Trichoderma for the preparation of a feed and/or formulation for shrimp for the treatment or prevention of Vibrio infection, said formulation comprising an excipient.
在本发明的一实施例中提供一种将木霉菌予以在制备用于治疗或预防弧菌感染的虾的饲料及/或制剂的应用，所述的赋形剂自豆粉、米糠、大豆粕、芝麻粕、篦麻籽粕、油菜籽粕、花生粕及麸皮所组成的群组中所选出的一个或多个。In one embodiment of the present invention, there is provided a use of Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection of shrimp, the excipient from soy flour, rice bran, soybean meal One or more selected from the group consisting of sesame meal, ricin seed meal, rapeseed meal, peanut meal and bran.
在本发明的一实施例中提供一种将木霉菌予以在制备用于治疗或预防弧菌感染的虾的饲料及/或制剂的应用，所述的制剂包括木霉菌、碳源、氮源及水。In an embodiment of the invention, there is provided a use of Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection, the preparation comprising Trichoderma, a carbon source, a nitrogen source and water.
在本发明的一实施例中提供一种将木霉菌予以在制备用于治疗或预防弧菌感染的虾的饲料及/或制剂的应用，所述的碳源与所述的菌粉的重量比为5:1～100:1、所述的氮源与所述的菌粉的重量比为5:1～100:1以及所述的水与所述的菌粉、所述的碳源及所述的氮源的总重量的重量比为5:1～50:1。In an embodiment of the present invention, there is provided a use of Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection, the weight ratio of said carbon source to said bacterial powder The weight ratio of the nitrogen source to the bacterial powder is 5:1 to 100:1, and the water and the bacterial powder, the carbon source and the The weight ratio of the total weight of the nitrogen source is from 5:1 to 50:1.
在本发明的一实施例中提供一种将木霉菌予以在制备用于治疗或预防弧菌感染的虾的饲料及/或制剂的应用，所述的制剂包括含有所述的木霉菌的菌粉、砂糖、米糠及水，其特征在于，所述的砂糖与所述的菌粉的重量比为20:1、所述的米糠与所述的菌粉的重量比为20:1以及所述的水与所述的菌粉、所述的砂糖及所述的米糠的总重量的重量比为19.51:1In an embodiment of the invention, there is provided a use of a Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection, the preparation comprising a mushroom powder comprising the Trichoderma , granulated sugar, rice bran and water, characterized in that the weight ratio of the granulated sugar to the bacterium powder is 20:1, the weight ratio of the rice bran to the bacterium powder is 20:1 and the The weight ratio of water to the total weight of the bacterin, the granulated sugar and the rice bran is 19.51:1
在本发明的一实施例中提供一种将木霉菌予以在制备用于治疗或预防弧菌感染的虾的饲料及/或制剂的应用，所述的木霉菌自绿色木霉菌(Trichoderma viride)、哈茨木霉菌(Trichoderma harzianum)、钩状木霉菌(Trichoderma hamatum)、长枝木霉菌(Trichoderma  longibrachiatum)、康氏木霉菌(Trichoderma koningii)、棘孢木霉菌(Trichoderma asperellum)、拟康氏木霉菌(Trichoderma pseudokoningii)、卷曲木霉菌(Trichoderma spirale)、顶孢木霉菌(Trichoderma fertile)、粗壮木霉菌(Trichoderma strigosum)及长孢木霉菌(Trichoderma longipile)组成的群组中所选出的一个或多个。In an embodiment of the invention, there is provided a use of Trichoderma viride, a Trichoderma viride, for the preparation of a feed and/or a preparation for the treatment or prevention of a Vibrio infection of a shrimp Trichoderma harzianum, Trichoderma hamatum, Trichoderma Longibrachiatum), Trichoderma koningii, Trichoderma asperellum, Trichoderma pseudokoningii, Trichoderma spirale, Trichoderma fertile, stout wood One or more selected from the group consisting of Trichoderma strigosum and Trichoderma longipile.
在本发明的一实施例中提供一种将木霉菌予以在制备用于治疗或预防弧菌感染的虾的饲料及/或制剂的应用，所述的弧菌感染为急性肝胰腺坏死综合症(Acute Hepatopancreatic Necrosis Syndrome,AHPNS)或非急性肝胰腺坏死综合症(Hepatopancreatic Necrosis Syndrome,HPNS)的弧菌感染。In an embodiment of the invention, there is provided a use of a Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection, the Vibrio infection being acute hepatopancreatic necrosis syndrome ( Acute Hepatopancreatic Necrosis Syndrome (AHPNS) or Vibrio infection of Hepatopancreatic Necrosis Syndrome (HPNS).
在本发明的一实施例中提供一种将木霉菌予以在制备用于治疗或预防弧菌感染的虾的饲料及/或制剂的应用，所述的弧菌为溶藻弧菌、鳗弧菌、副溶血弧菌、创伤弧菌或霍乱弧菌。In an embodiment of the present invention, there is provided a use of Trichoderma sp. for preparing a feed and/or a preparation for treating or preventing a Vibrio infection of a shrimp, the Vibrio algae, Vibrio anguillarum , Vibrio parahaemolyticus, Vibrio vulnificus or Vibrio cholerae.
经由本发明所采用的技术手段，虾的饲料及/或制剂含有木霉菌，并经投予至虾养殖池后可有效地治疗或预防所述的受弧菌感染的虾，且不对所述的虾造成任何副作用。According to the technical means adopted by the present invention, the shrimp feed and/or preparation contains Trichoderma, and after being administered to the shrimp culture pond, the Spirulina-infected shrimp can be effectively treated or prevented, and the Shrimp causes any side effects.
具体实施方式detailed description
以下说明并非为限制本发明的实施方式，而为本发明的实施例的一种。The following description is not intended to limit the embodiments of the present invention, but is an embodiment of the invention.
本发明的一第一实施例为将木霉菌予以在制备用于治疗或预防弧菌感染的虾的饲料的应用。A first embodiment of the present invention is the use of Trichoderma for the preparation of a feed for shrimp for the treatment or prevention of Vibrio infection.
在本实施例中，所述的木霉菌为绿色木霉菌。当然，本发明不以此为限。在其它实施例中，木霉菌可从由哈茨木霉菌(Trichoderma harzianum)、钩状木霉菌(Trichoderma hamatum)、长枝木霉菌(Trichoderma longibrachiatum)、康氏木霉菌(Trichoderma koningii)、棘孢木霉菌(Trichoderma asperellum)、拟康氏木霉菌(Trichoderma pseudokoningii)、卷曲木霉菌(Trichoderma spirale)、顶孢木霉菌(Trichoderma fertile)、粗壮木霉菌(Trichoderma strigosum)及长孢木霉菌(Trichoderma longipile)组成的群组中所选出的一个或多个。In this embodiment, the Trichoderma is Trichoderma viride. Of course, the invention is not limited thereto. In other embodiments, the Trichoderma can be from Trichoderma harzianum, Trichoderma hamatum, Trichoderma longibrachiatum, Trichoderma koningii, Trichoderma oxysporum (Trichoderma asperellum), Trichoderma pseudokoningii, Trichoderma spirale, Trichoderma fertile, Trichoderma strigosum, and Trichoderma longipile One or more selected in the group.
详细而言，所述的弧菌可为溶藻弧菌、鳗弧菌、副溶血弧菌、创伤弧菌或霍乱弧菌。以及，所述的弧菌感染可为急性肝胰腺坏死综合症(Acute Hepatopancreatic Necrosis Syndrome,AHPNS)或非急性肝胰腺坏死综合症(Hepatopancreatic Necrosis Syndrome,HPNS)的弧菌感染。In detail, the Vibrio may be Vibrio alginolyticus, Vibrio anguillarum, Vibrio parahaemolyticus, Vibrio vulnificus or Vibrio cholerae. And, the Vibrio infection may be a Vibrio infection of Acute Hepatopancreatic Necrosis Syndrome (AHPNS) or Hepatopancreatic Necrosis Syndrome (HPNS).
本实施例中，所述的饲料为干性颗粒的饲料。选择性地，所述的饲料可为液态的饲料。较佳地，所述的饲料为经发酵处理的饲料而易于消化吸收。 In this embodiment, the feed is a dry granule feed. Alternatively, the feed may be a liquid feed. Preferably, the feed is fermented feed and is easily digested and absorbed.
较佳地，在本实施例中，每公斤的所述的饲料中添加有1克的所述的木霉菌，且所述的木霉菌的菌量为5×108cfu/g。当然，本发明不以此为限。在其它实施例中，每公斤饲料所含的木霉菌的重量可在0.01克～200克并包括0.01克及200克，而所述的木霉菌的菌量可在1×102cfu/g～5×1010cfu/g并包括1×102cfu/g及5×1010cfu/g。Preferably, in the present embodiment, 1 gram of said Trichoderma is added per kg of said feed, and said Trichoderma has a bacterial amount of 5 × 10 8 cfu/g. Of course, the invention is not limited thereto. In other embodiments, the weight of Trichoderma per kg of feed may range from 0.01 g to 200 g and includes 0.01 g and 200 g, and the amount of the Trichoderma may be 1×10 2 cfu/g～ 5 × 10 10 cfu / g and includes 1 × 10 2 cfu / g and 5 × 10 10 cfu / g.
上述经制备而含有绿色木霉菌的所述的饲料，透过直接喂食的方式给予患有急性肝胰腺坏死综合症但还具有进食能力的白虾。并在喂食后的三天观察所述的虾的肝脏、胰脏及肠线，得到原本因罹病而白化变淡的所述的肝脏、所述的胰脏及所述的肠线恢复成正常的深黑色的结果。The above-mentioned feed prepared to contain Trichoderma viride is administered by direct feeding to white shrimp having acute hepatopancreatic necrosis syndrome but also having feeding ability. And the liver, pancreas and gut of the shrimp were observed three days after the feeding, and the liver, the pancreas and the gut which were originally whitened and weakened by rickets were restored to normal. Dark black result.
本发明的一第二实施例为将木霉菌予以在制备用于治疗或预防弧菌感染的虾的制剂的应用。A second embodiment of the present invention is the use of Trichoderma for the preparation of a shrimp for the treatment or prevention of Vibrio infection.
本发明的含有木霉菌的制剂的制备过程如下。The preparation process of the Trichoderma-containing preparation of the present invention is as follows.
将5×108cfu/g的绿色木霉菌菌粉与碳源、氮源及蒸馏水相混合，并以5liter/min进行曝气，而在72小时后得到木霉菌的菌量为5×109cfu/g的菌液，亦即所述的含有木霉菌的制剂。详细而言，本实施例中所采用的所述的碳源为砂糖，且重量为所述的木霉菌菌粉的20倍；而采用的所述的氮源为米糠，且重量为所述的木霉菌菌粉的20倍；以及所述的蒸馏水的重量为所述的木霉菌菌粉、所述的砂糖与所述的米糠的总重量的19.51倍。当然，本发明不以木霉菌的菌量为5×109cfu/g为限。木霉菌的菌量可在1×102cfu/g～5×1010cfu/g并包括1×102cfu/g及5×1010cfu/g。此外，在其它实施例中，所述的碳源与所述的菌粉的重量比可为5:1～100:1、所述的氮源与所述的菌粉的重量比可为5:1～100:1以及所述的水与所述的菌粉、所述的碳源及所述的氮源的总重量的重量比可为5:1～50:1。在其它实施例中，木霉菌可经喷附而在赋形剂上干燥形成菌量落在1×102cfu/g～5×1010cfu/g的制剂。所述的赋形剂自豆粉、米糠、大豆粕、芝麻粕、篦麻籽粕、油菜籽粕、花生粕及麸皮所组成的群组中所选出的一个或多个。5×10 8 cfu/g of Trichoderma viride powder was mixed with carbon source, nitrogen source and distilled water, and aerated at 5 liter/min, and the amount of Trichoderma obtained after 72 hours was 5×10 9 . The bacterial liquid of cfu/g, that is, the preparation containing Trichoderma. In detail, the carbon source used in the embodiment is granulated sugar and the weight is 20 times that of the Trichoderma bacterium powder; and the nitrogen source used is rice bran, and the weight is as described. 20 times of the Trichoderma powder; and the weight of the distilled water is 19.51 times the total weight of the Trichoderma powder, the sugar and the rice bran. Of course, the present invention is not limited to the amount of Trichoderma bacteria of 5 × 10 9 cfu / g. The amount of the fungus of Trichoderma can be from 1 × 10 2 cfu / g to 5 × 10 10 cfu / g and includes 1 × 10 2 cfu / g and 5 × 10 10 cfu / g. In addition, in other embodiments, the weight ratio of the carbon source to the bacterial powder may be 5:1 to 100:1, and the weight ratio of the nitrogen source to the bacterial powder may be 5: The weight ratio of 1 to 100:1 and the total weight of the water and the bacterial powder, the carbon source, and the nitrogen source may be 5:1 to 50:1. In other embodiments, Trichoderma can be sprayed and dried on an excipient to form a formulation having a bacterial count of from 1 x 10 2 cfu/g to 5 x 10 10 cfu/g. The excipient is one or more selected from the group consisting of soy flour, rice bran, soybean meal, sesame meal, castor seed meal, rapeseed meal, peanut meal and bran.
所述的制剂直接投入已发生急性肝胰腺坏死综合症的白虾养殖池，所述的制剂的投入量与虾子的恢复时间呈现出反比关。例如，使得所述的养殖池的菌液(制剂)浓度为4ppm，则三天后可得到白虾原本因罹病而白化变淡的所述的肝脏、所述的胰脏及所述的肠线已恢复成正常的深黑色，并可正常摄食的功效。而若使养殖池的菌液(制剂)浓度为2ppm，则需至少六天才能得到有效的治疗效果。The preparation directly inputs the white shrimp culture pond in which acute hepatopancreatic necrosis syndrome has occurred, and the input amount of the preparation is inversely related to the recovery time of the shrimp. For example, if the concentration of the bacterial liquid (formulation) in the culture pond is 4 ppm, the liver, the pancreas, and the gut line of the white shrimp originally whitened and weakened due to rickets may be obtained after three days. Restores to normal dark black and can be used for normal feeding. If the concentration of the bacterial solution (formulation) in the culture pond is 2 ppm, it takes at least six days to obtain an effective therapeutic effect.
除了上述的白虾，本发明的含有木霉菌的饲料或制剂对其他虾类同样有相同或类似的治疗或预防效果。 In addition to the above-mentioned white shrimp, the Trichoderma-containing feed or preparation of the present invention has the same or similar therapeutic or prophylactic effects on other shrimps.
以上的叙述以及说明仅为本发明的较佳实施例的说明，对于本领域普通技术人员当可依据以上所界定权利要求书以及上述的说明而作其他的修改，只要这些修改仍应是为本发明的发明精神而在本发明的权利范围中。 The above description and description are only illustrative of the preferred embodiments of the present invention, and other modifications may be made by those skilled in the art in light of the above defined claims and the description above, as long as the modifications are still The inventive spirit of the invention is within the scope of the invention.

Claims (10)

  1. 一种将木霉菌予以在制备用于治疗或预防弧菌感染的虾的饲料及/或制剂的应用。A use of Trichoderma for the preparation of a feed and/or preparation for shrimp for the treatment or prevention of Vibrio infection.
  2. 如权利要求1所述的应用，其特征在于，该饲料包括干性颗粒的饲料或经发酵处理的液态饲料。The use according to claim 1, wherein the feed comprises a dry granule feed or a fermented liquid feed.
  3. 如权利要求1所述的应用，其特征在于，每公斤的该饲料中添加有0.01克～300克的该木霉菌，且该木霉菌的菌量为1×102cfu/g～5×1010cfu/g。The use according to claim 1, wherein 0.01 to 300 g of the Trichoderma is added per kg of the feed, and the amount of the Trichoderma is 1 × 10 2 cfu / g - 5 × 10 10 cfu/g.
  4. 如权利要求1所述的应用，其特征在于，每公斤的该饲料中添加有0.1克的该木霉菌，且该木霉菌的菌量为5×108cfu/g。The use according to claim 1, wherein 0.1 g of the Trichoderma is added per kg of the feed, and the Trichoderma has a bacterial amount of 5 × 10 8 cfu/g.
  5. 如权利要求1所述的应用，其特征在于，该制剂中的该木霉菌的菌量为1×102cfu/g～5×1010cfu/g。The use according to Claim 1, wherein the Trichoderma in the preparation has a bacterial amount of from 1 × 10 2 cfu / g to 5 × 10 10 cfu / g.
  6. 如权利要求1所述的应用，其特征在于，该制剂包括赋形剂。The use according to claim 1 wherein the formulation comprises an excipient.
  7. 如权利要求6所述的应用，其特征在于，该赋形剂自豆粉、米糠、大豆粕、芝麻粕、篦麻籽粕、油菜籽粕、花生粕及麸皮所组成的群组中所选出的一个或多个。The use according to claim 6, wherein the excipient is in the group consisting of soy flour, rice bran, soybean meal, sesame meal, castor bean meal, rapeseed meal, peanut meal and bran. One or more selected.
  8. 如权利要求1所述的应用，其特征在于，该制剂包括木霉菌、碳源、氮源及水。The use according to claim 1 wherein the formulation comprises Trichoderma, a carbon source, a nitrogen source and water.
  9. 如权利要求8所述的应用，其特征在于，该碳源与该菌粉的重量比为5:1～100:1、该氮源与该菌粉的重量比为5:1～100:1以及该水与该菌粉、该碳源及该氮源的总重量的重量比为5:1～50:1。The application according to claim 8, wherein the weight ratio of the carbon source to the bacterial powder is 5:1 to 100:1, and the weight ratio of the nitrogen source to the bacterial powder is 5:1 to 100:1. And the weight ratio of the water to the bacterial powder, the carbon source, and the total weight of the nitrogen source is 5:1 to 50:1.
  10. 如权利要求1所述的应用，其特征在于，该木霉菌自绿色木霉菌、哈茨木霉菌、钩状木霉菌、长枝木霉菌、康氏木霉菌、棘孢木霉菌、拟康氏木霉菌、卷曲木霉菌、顶孢木霉菌、粗壮木霉菌及长孢木霉菌组成的群组中所选出的一个或多个。 The use according to claim 1, wherein the Trichoderma from Trichoderma viride, Trichoderma harzianum, Trichoderma harzianum, Trichoderma longum, Trichoderma koningii, Trichoderma oxysporum, Trichoderma koningii One or more selected from the group consisting of Trichoderma spp., Trichoderma sp., Trichoderma sp., and Trichoderma sp.
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