TW201735795A - An application of Trichoderma to a preparation of a feed and/or a preparation for treating or preventing vibrio infection in a shrimp - Google Patents

Abstract

Disclosed is an application of Trichoderma to a preparation of a feed and/or a preparation for treating or preventing vibrio infection in a shrimp, wherein the feed is dry granules or a fermented feed, and 0.01 grams~200 grams of the Trichoderma is added to each kilogram of the feed, and the bacterial count of the Trichoderma in the feed is from 1*10<SP>2</SP> cfu/g to 5*10<SP>10</SP> cfu/g, and the bacterial count of the Trichoderma in the preparation is from 1*10<SP>2</SP> cfu/g to 5*10<SP>10</SP> cfu/g.

Description

Application of Trichoderma in the preparation of feed and/or preparation of shrimp for treating or preventing Vibrio infection

The present invention relates to a feed and/or formulation for administration to shrimp, and more particularly to the use of a mold and/or formulation for the preparation of a shrimp for the treatment or prevention of a Vibrio infection.

Vibrio is one of the common bacterial groups in the marine environment, and it has strong adaptability and resistance to become a dominant population in the seawater environment. The disease caused by Vibrio is a bacterial disease that is prevalent and extremely harmful in aquatic animals such as fish, shrimp, crab and shellfish, which can cause serious economic losses in the aquaculture industry.

Vibrio belongs to Gram-negative bacteria, and Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio vulnificus or Vibrio cholerae can infect shrimp. For example, a shrimp infected with Acute Hepatopancreatic Necrosis Syndrome (AHPNS), which is infected with Vibrio parahaemolyticus, produces a toxin in the hepatopancreas of shrimps after being infected by Vibrio parahaemolyticus. Causes hepatic and pancreatic lesions and functional disorders, including slow growth, spiral swimming, shell damage, hepatopancreas redness, swelling, and even atrophy and necrosis; more often, hepatic pancreatic duct epithelium, pancreatic microvilli tissue of shrimp The intestinal mucosa epithelium and proliferating cells form white organic matter which forms white stool in the intestinal tract.

In the past, the treatment or prevention of shrimp infected with Vibrio was mainly carried out by administering antibiotics, nutrients and chemical disinfectants. However, these practices still have the following disadvantages.

First, the administration of antibiotics to the culture ponds will not be effective after the bacteria develop resistance, and failure to comply with the withdrawal schedule will result in the antibiotics remaining in the shrimps, which will threaten the health of the people who consume the shrimps. In addition, if the culture water used for antibiotics is not properly disposed, it may cause drug contamination in coastal waters and inland areas. Feeding vitamin C, polysaccharides, herbal agents and other nutrients that help boost the immunity of shrimps does not work for already infected shrimps, nor does it inhibit Vibrio. As for the use of chemical disinfectants, only Vibrio in the culture pond can be killed, and it is impossible to produce an effective therapeutic effect on the Vibrio and the lesions caused by the shrimp.

Accordingly, it is an object of the present invention to provide an application of a Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection, which can be effectively treated without causing side effects or injuries to the shrimp. Or shrimp that are protected from Vibrio infection and its associated pathology.

The technical means employed by the present invention to solve the problems of the prior art provides a use of Trichoderma for the preparation of feeds and/or preparations for shrimp for the treatment or prevention of Vibrio infection.

In one embodiment of the invention, there is provided a use of a Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection, the feed comprising a dry granule feed or a fermented liquid Feed.

In an embodiment of the present invention, there is provided a method for preparing Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection, and adding 0.01 to 200 g per kg of the feed. The Trichoderma, and the amount of the Trichoderma strain is 1 × 10 ² cfu / g ~ 5 × 10 ¹⁰ cfu / g.

In one embodiment of the present invention, there is provided an application of Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection, wherein 0.1 kg of the wood is added per kg of the feed. Mold, and the amount of the Trichoderma strain was 5 × 10 ⁸ cfu / g.

In an embodiment of the present invention, there is provided an application of a Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection, wherein the Trichoderma strain is 1×. 10 ² cfu/g ~5×10 ¹⁰ cfu/g.

In one embodiment of the invention, there is provided the use of Trichoderma for the preparation of a feed and/or formulation of a shrimp for the treatment or prevention of a Vibrio infection comprising an excipient.

In one embodiment of the present invention, there is provided a use of Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection of shrimp, the excipient being from soy flour, rice bran, soybean meal, One or more selected from the group consisting of sesame meal, castor seed meal, rapeseed meal, peanut meal and bran.

In one embodiment of the invention, there is provided a use of Trichoderma for the preparation of a feed and/or formulation for shrimp for the treatment or prevention of Vibrio infection, the preparation comprising Trichoderma, a carbon source, a nitrogen source and water.

In one embodiment of the present invention, there is provided an application of Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection, the weight ratio of the carbon source to the bacterial powder being 5: 1~100:1, the weight ratio of the nitrogen source to the bacterial powder is 5:1~100:1 and the weight ratio of the water to the bacterial powder, the carbon source and the total weight of the nitrogen source is 5: 1~50:1.

In one embodiment of the present invention, there is provided an application of a Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection, the preparation comprising a fungus powder, sugar, rice bran containing the Trichoderma And water, wherein the weight ratio of the sugar to the bacterial powder is 20:1, the weight ratio of the rice bran to the bacterial powder is 20:1, and the total weight of the water and the bacterial powder, the sugar and the rice bran The weight ratio is 19.51:1

In one embodiment of the invention, there is provided the use of a Trichoderma viride in the preparation of a feed and/or a preparation for the treatment of a shrimp for the treatment or prevention of a Vibrio infection, Trichoderma viride, Ha Trichoderma harzianum, Trichoderma hamatum, Trichoderma longibrachiatum, Trichoderma koningii, Trichoderma asperellum, Trichoderma One or more selected from the group consisting of pseudokoningii), Trichoderma spirale, Trichoderma fertile, Trichoderma strigosum, and Trichoderma longipile.

In one embodiment of the present invention, there is provided a use of Trichoderma for the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection, the Vibrio infection is Acute Hepatopancreatic Necrosis Syndrome (Acute) Vibrio infection of Hepatopancreatic Necrosis Syndrome (AHPNS) or Hepatopancreatic Necrosis Syndrome (HPNS).

In one embodiment of the present invention, there is provided an application of Trichoderma in the preparation of a feed and/or a preparation for treating or preventing a Vibrio infection, the Vibrio system being Vibrio alginolyticus, Vibrio anguillarum, Vibrio parahaemolyticus, Vibrio vulnificus or Vibrio cholerae.

According to the technical means adopted by the present invention, the shrimp feed and/or preparation contains Trichoderma, and after being administered to the shrimp culture pond, the Vibrio infected shrimp can be effectively treated or prevented without causing any side effect.

The following description is not intended to limit the embodiments of the present invention, but is an embodiment of the invention.

A first embodiment of the present invention is the use of Trichoderma for the preparation of a feed for shrimp for the treatment or prevention of Vibrio infection.

In this embodiment, the Trichoderma strain is Trichoderma viride. Of course, the invention is not limited thereto. In other embodiments, the Trichoderma can be from Trichoderma harzianum, Trichoderma hamatum, Trichoderma longibrachiatum, Trichoderma koningii, Trichoderma spp. (Trichoderma asperellum), Trichoderma pseudokoningii, Trichoderma spirale, Trichoderma fertile, Trichoderma strigosum, and Trichoderma longipile One or more selected in the group.

In detail, the Vibrio may be Vibrio alginolyticus, Vibrio anguillarum, Vibrio parahaemolyticus, Vibrio vulnificus or Vibrio cholerae. And the Vibrio infection may be a Vibrio infection of Acute Hepatopancreatic Necrosis Syndrome (AHPNS) or Hepatopancreatic Necrosis Syndrome (HPNS).

In this embodiment, the feed is a dry granule feed. Alternatively, the feed can be a liquid feed. Preferably, the feed is a fermented feed which is easily digested and absorbed.

Preferably, in the present embodiment, 1 gram of the Trichoderma is added per kilogram of the feed, and the amount of the Trichoderma is 5×10 ⁸ cfu/g. Of course, the invention is not limited thereto. In other embodiments, the weight of Trichoderma per kg of feed may range from 0.01 g to 200 g and includes 0.01 g and 200 g, and the amount of the Trichoderma may be 1×10 ² cfu/g~5×. 10 ¹⁰ cfu/g and includes 1×10 ² cfu/g and 5×10 ¹⁰ cfu/g.

The feed prepared as described above containing Trichoderma viride is administered to white shrimp having acute hepatopancreatic necrosis syndrome but also having feeding ability by direct feeding. The liver, pancreas and gut of the shrimp were observed three days after the feeding, and the liver, the pancreas and the gut which were originally whitened and weakened by rickets were restored to a normal dark black.

A second embodiment of the present invention is the use of Trichoderma for the preparation of a shrimp for the treatment or prevention of Vibrio infection.

The preparation process of the Trichoderma-containing preparation of the present invention is as follows.

5×10 ⁸ cfu/g of Trichoderma viride powder was mixed with carbon source, nitrogen source and distilled water, and aerated at 5 liter/min, and the amount of Trichoderma obtained after 72 hours was 5×10. ⁹ cfu / g of bacterial liquid, that is, the preparation containing Trichoderma. In detail, the carbon source used in the embodiment is granulated sugar and the weight is 20 times that of the Trichoderma powder; and the nitrogen source used is rice bran, and the weight is 20% of the Trichoderma powder. And the weight of the distilled water is 19.51 times the total weight of the Trichoderma powder, the sugar and the rice bran. Of course, the present invention is not limited to the amount of Trichoderma bacteria of 5 × 10 ⁹ cfu / g. The amount of Trichoderma can be from 1 × 10 ² cfu / g to 5 × 10 ¹⁰ cfu / g and includes 1 × 10 ² cfu / g and 5 × 10 ¹⁰ cfu / g. In addition, in other embodiments, the weight ratio of the carbon source to the bacterial powder may be 5:1 to 100:1, and the weight ratio of the nitrogen source to the bacterial powder may be 5:1 to 100:1 and the water. The weight ratio to the bacterial powder, the carbon source, and the total weight of the nitrogen source may be from 5:1 to 50:1. In other embodiments, Trichoderma can be sprayed and dried on an excipient to form a formulation having a bacterial count falling between 1 x 10 ² cfu/g and 5 x 10 ¹⁰ cfu/g. The excipient is one or more selected from the group consisting of soy flour, rice bran, soybean meal, sesame meal, castor seed meal, rapeseed meal, peanut meal and bran.

The preparation is directly applied to the white shrimp culture pond where acute hepatopancreatic necrosis syndrome has occurred, and the input amount of the preparation is inversely related to the recovery time of the shrimp. For example, if the concentration of the bacterial liquid (preparation) in the culture pond is 4 ppm, the liver, the pancreas and the gut which have been whitened and blanched due to rickets may be obtained after three days, and the gut has returned to a normal dark black color. It can also be used for normal feeding. If the concentration of the bacterial solution (formulation) in the culture pond is 2 ppm, it takes at least six days to obtain an effective therapeutic effect.

In addition to the above-mentioned white shrimp, the feed or preparation containing Trichoderma of the present invention has the same or similar therapeutic or prophylactic effects on other shrimps.

The above description and description are only illustrative of the preferred embodiments of the present invention, and those of ordinary skill in the art can make other modifications in accordance with the scope of the invention as defined below and the description above, but such modifications should still be It is within the scope of the invention to the invention of the invention.

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Claims (10)

A use of Trichoderma for the preparation of a feed and/or preparation for shrimp for the treatment or prevention of Vibrio infection. The application of claim 1, wherein the feed comprises a dry granule feed or a lyophilized liquid feed. The application according to claim 1, wherein 0.01 to 300 g of the Trichoderma is added per kg of the feed, and the strain of the Trichoderma is 1×10 ² cfu/g to 5×10 ¹⁰ Cfu/g. The application of claim 1, wherein 0.1 g of the Trichoderma is added per kg of the feed, and the Trichoderma strain is 5 × 10 ⁸ cfu/g. The application according to claim 1, wherein the amount of the Trichoderma in the preparation is from 1 × 10 ² cfu / g to 5 × 10 ¹⁰ cfu / g. The use of claim 1 wherein the formulation comprises an excipient. The application of claim 6, wherein the excipient is selected from the group consisting of soy flour, rice bran, soybean meal, sesame meal, castor bean meal, rapeseed meal, peanut meal and bran. one or more. The use of claim 1, wherein the formulation comprises Trichoderma, a carbon source, a nitrogen source, and water. The application of claim 8, wherein the weight ratio of the carbon source to the bacterial powder is 5:1 to 100:1, and the weight ratio of the nitrogen source to the bacterial powder is 5:1 to 100:1. The weight ratio of the water to the total weight of the bacterial powder, the carbon source and the nitrogen source is 5:1 to 50:1. The application of claim 1, wherein the Trichoderma strain is from Trichoderma viride, Trichoderma harzianum, Trichoderma hamatum, Trichoderma longibrachiatum, Kang. Trichoderma koningii, Trichoderma asperellum, Trichoderma pseudokoningii, Trichoderma spirale, Trichoderma fertile, Trichoderma strigosum And one or more selected from the group consisting of Trichoderma longipile.