Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
  • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
  • C07D209/04—Indoles; Hydrogenated indoles
  • C07D209/30—Indoles; Hydrogenated indoles with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to carbon atoms of the hetero ring
  • C07D209/42—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
  • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
  • C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
  • C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
  • C07D413/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings linked by a chain containing hetero atoms as chain links

Definitions

• the present invention relates to compounds and methods useful for inhibiting 3- phosphoglycerate dehydrogenase.
• the invention also provides pharmaceutically acceptable compositions comprising compounds of the present invention and methods of using said compositions in the treatment of various disorders.
• Phosphoglycerate dehydrogenase catalyzes the first step in the biosynthesis of L-serine, which is the conversion of 3 -phosphoglycerate into 3-phosphohydroxypyruvate with a reduction of nicotinamide adenine dinucleotide (NAD + ) to NADH.
• Certain cancers including human melanomas and breast cancers, can have high levels of PHGDH. These cancer cells are dependent on PHGDH for their growth and survival as PHGDH catalyzes serine production and may also be a significant source of NADPH in cancer cells. Targeting PHGDH by small molecule inhibitors could be a therapeutic strategy to reduce cancer cell growth and survival. Accordingly, there remains a need to find PHGDH inhibitors useful as therapeutic agents.
• R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , and L 1 is as defined and described herein.
• Compounds of the present invention are useful for treating a variety of diseases, disorders or conditions, associated with PHGDH.
• diseases, disorders, or conditions include cellular proliferative disorders (e.g., cancer) such as those described herein.
• Compounds of the present invention, and compositions thereof are useful as inhibitors of PHGDH. Without wishing to be bound by any particular theory, it is believed that compounds of the present invention, and compositions thereof, may inhibit the activity of PHGDH and/or inhibit the production of NADPH, and thus reduce the growth of cells in proliferative disorders such as cancer.
• the present invention provides a compound of formula I:
• R 1 is hydrogen or Ci-4 alkyl
• each of R 2 and R 3 is independently halogen, -OR, -CN, Ci-6 aliphatic optionally substituted with 1, 2, or 3 halogens, or -L-R'; or R 2 and R 3 are optionally taken together with the carbon atoms to which they are attached and any intervening atoms to form a 5-8 membered partially unsaturated ring having 0-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
• R 4 is hydrogen, halogen, -OR 5 , -CN, Ci-6 aliphatic optionally substituted with 1, 2, or 3 halogens, or -L-R'; each R is independently hydrogen or an optionally substituted group selected from Ci-6 aliphatic, a 3-8 membered saturated or partially unsaturated monocyclic carbocyclic ring, phenyl, an 8-10 membered bicyclic aromatic carbocyclic ring, a 4-8 membered saturated or partially unsaturated monocyclic heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur, a 5-6 membered monocyclic heteroaromatic ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or an 8-10 membered bicyclic heteroaromatic ring having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
• R 5 is hydrogen, -(CH2) n -phenyl, or Ci-6 alkyl optionally substituted with 1, 2, or 3 halogens; each L is independently a Ci-6 bivalent straight or branched hydrocarbon chain wherein 1-4 methylene units of the chain are independently and optionally replaced with -0-, -C(O)-, - C(0)0-, -OC(O)-, -OC(0)N(R)-, -(R)NC(0)0-, -C(0)N(R)-, -(R)NC(O)-, -N(R)-, - N(R)C(0)N(R)-, -S-, -SO-, or -S0 2 -;
• each R' is independently hydrogen, Ci-6 aliphatic, or an optionally substituted 4-8 membered saturated or partially unsaturated monocyclic heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
• R 6 is hydrogen or Ci-4 alkyl
• R 7 is hydrogen, -C0 2 R, Ci- 6 optionally substituted aliphatic group, or a bivalent 3-7 membered ring;
• L 1 is a covalent bond or a Ci -8 bivalent straight or branched hydrocarbon chain wherein 1-5 methylene units of the chain are independently and optionally replaced with -0-, -C(O)-, - C(0)0-, -OC(O)-, -N(R)-, -C(0)N(R)-, -(R)NC(O)-, -OC(0)N(R)-, -(R)NC(0)0-, - N(R)C(0)N(R)-, -S-, -SO-, -SO2-, -S0 2 N(R)-, -(R)NS0 2 -, -C(S)-, -C(S)0-, -OC(S)-, - C(S)N(R)-, -(R)NC(S)-, -(R)NC(S)N(R)-, or -Cy-;
• each -Cy- is independently a bivalent 6-membered arylene ring containing 0-2 nitrogen atoms , or a bivalent 5-membered heteroarylene ring with 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a bivalent partially unsaturated 8-10 membered bicyclic heterocyclene ring with 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein -Cy- is optionally substituted with 1 or 2 substituents independently selected from Ci-4 alkyl or -OR;
• R 8 is hydrogen, -CO2R, or Ci-6 optionally substituted aliphatic;
• R 9 is hydrogen, halogen, C 1-4 alkyl, -CN, -OR, -(CH2) n -(optionally substituted phenyl), or L 2 -R 8 ;
• each L 2 is independently Ci -8 bivalent straight or branched hydrocarbon chain wherein 1-4 methylene units of the chain are independently and optionally replaced with -0-, -C(O)-, - C(0)0-, -OC(O)-, -N(R)-, -C(0)N(R)-, -(R)NC(O)-, -OC(0)N(R)-, -(R)NC(0)0-, - N(R)C(0)N(R)-, -S-, -SO-, -SO2-, -S0 2 N(R)-, -(R)NS0 2 -, -C(S)-, -C(S)
• n is independently 0, 1, 2, 3, 4, or 5.
• aliphatic or "aliphatic group”, as used herein, means a straight-chain (i.e., unbranched) or branched, substituted or unsubstituted hydrocarbon chain that is completely saturated or that contains one or more units of unsaturation, or a monocyclic hydrocarbon or bicyclic hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but which is not aromatic (also referred to herein as "carbocycle,” “cycloaliphatic” or “cycloalkyl”), that has a single point of attachment to the rest of the molecule.
• aliphatic groups contain 1-6 aliphatic carbon atoms.
• aliphatic groups contain 1-5 aliphatic carbon atoms. In other embodiments, aliphatic groups contain 1-4 aliphatic carbon atoms. In still other embodiments, aliphatic groups contain 1-3 aliphatic carbon atoms, and in yet other embodiments, aliphatic groups contain 1-2 aliphatic carbon atoms.
• cycloaliphatic (or “carbocycle” or “cycloalkyl”) refers to a monocyclic C3-C6 hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but which is not aromatic, that has a single point of attachment to the rest of the molecule.
• Suitable aliphatic groups include, but are not limited to, linear or branched, substituted or unsubstituted alkyl, alkenyl, alkynyl groups and hybrids thereof such as (cycloalkyl)alkyl, (cycloalkenyl)alkyl or (cycloalkyl)alkenyl.
• bridged bicyclic refers to any bicyclic ring system, i.e. carbocyclic or heterocyclic, saturated or partially unsaturated, having at least one bridge.
• a "bridge” is an unbranched chain of atoms or an atom or a valence bond connecting two bridgeheads, where a "bridgehead” is any skeletal atom of the ring system which is bonded to three or more skeletal atoms (excluding hydrogen).
• a bridged bicyclic group has 7-12 ring members and 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• bridged bicyclic groups are well known in the art and include those groups set forth below where each group is attached to the rest of the molecule at any substitutable carbon or nitrogen atom. Unless otherwise specified, a bridged bicyclic group is optionally substituted with one or more substituents as set forth for aliphatic groups. Additionally or alternatively, any substitutable nitrogen of a bridged bicyclic group is optionally substituted. Exemplary bridged bicyclics include:
• lower alkyl refers to a C 1-4 straight or branched alkyl group.
• exemplary lower alkyl groups are methyl, ethyl, propyl, isopropyl, butyl, isobutyl, and tert-butyl.
• lower haloalkyl refers to a C 1-4 straight or branched alkyl group that is substituted with one or more halogen atoms.
• heteroatom means one or more of oxygen, sulfur, nitrogen, phosphorus, or silicon (including, any oxidized form of nitrogen, sulfur, phosphorus, or silicon; the quaternized form of any basic nitrogen or; a substitutable nitrogen of a heterocyclic ring, for example N (as in 3,4-dihydro-2H-pyrrolyl), H (as in pyrrolidinyl) or R + (as in N-substituted pyrrolidinyl)).
• Ci -8 saturated or unsaturated, straight or branched, hydrocarbon chain
• bivalent Ci -8 or Ci-6) saturated or unsaturated, straight or branched, hydrocarbon chain
• alkenylene or alkynylene chains that are straight or branched as defined herein.
• alkylene refers to a bivalent alkyl group.
• An "alkylene chain” is a polymethylene group, i.e., -(CH 2 )n-, wherein n is a positive integer, preferably from 1 to 6, from 1 to 4, from 1 to 3, from 1 to 2, or from 2 to 3.
• a substituted alkylene chain is a polymethylene group in which one or more methylene hydrogen atoms are replaced with a substituent. Suitable substituents include those described below for a substituted aliphatic group.
• alkenylene refers to a bivalent alkenyl group.
• a substituted alkenylene chain is a polymethylene group containing at least one double bond in which one or more hydrogen atoms are replaced with a substituent. Suitable substituents include those described below for a substituted aliphatic group.
• cyclopropylenyl refers to a bivalent cyclopropyl group of the following structure:
• halogen means F, CI, Br, or I.
• aryl used alone or as part of a larger moiety as in “aralkyl,” “aralkoxy,” or “aryloxyalkyl,” refers to monocyclic or bicyclic ring systems having a total of five to fourteen ring members, wherein at least one ring in the system is aromatic and wherein each ring in the system contains 3 to 7 ring members.
• aryl may be used interchangeably with the term “aryl ring.”
• aryl refers to an aromatic ring system which includes, but not limited to, phenyl, biphenyl, naphthyl, anthracyl and the like, which may bear one or more substituents.
• aryl is a group in which an aromatic ring is fused to one or more non-aromatic rings, such as indanyl, phthalimidyl, naphthimidyl, phenanthridinyl, or tetrahydronaphthyl, and the like.
• heteroaryl and “heteroar-,” used alone or as part of a larger moiety, e.g., “heteroaralkyl,” or “heteroaralkoxy,” refer to groups having 5 to 10 ring atoms, preferably 5, 6, or 9 ring atoms; having 6, 10, or 14 ⁇ electrons shared in a cyclic array; and having, in addition to carbon atoms, from one to five heteroatoms.
• heteroatom refers to nitrogen, oxygen, or sulfur, and includes any oxidized form of nitrogen or sulfur, and any quaternized form of a basic nitrogen.
• Heteroaryl groups include, without limitation, thienyl, furanyl, pyrrolyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, oxazolyl, isoxazolyl, oxadiazolyl, thiazolyl, isothiazolyl, thiadiazolyl, pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, indolizinyl, purinyl, naphthyridinyl, and pteridinyl.
• heteroaryl and “heteroar-”, as used herein, also include groups in which a heteroaromatic ring is fused to one or more aryl, cycloaliphatic, or heterocyclyl rings, where the radical or point of attachment is on the heteroaromatic ring.
• Nonlimiting examples include indolyl, isoindolyl, benzothienyl, benzofuranyl, dibenzofuranyl, indazolyl, benzimidazolyl, benzthiazolyl, quinolyl, isoquinolyl, cinnolinyl, phthalazinyl, quinazolinyl, quinoxalinyl, 4H-quinolizinyl, carbazolyl, acridinyl, phenazinyl, phenothiazinyl, phenoxazinyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl, and pyrido[2,3-b]-l,4-oxazin-3(4H)-one.
• heteroaryl group may be mono- or bicyclic.
• heteroaryl may be used interchangeably with the terms “heteroaryl ring,” “heteroaryl group,” or “heteroaromatic,” any of which terms include rings that are optionally substituted.
• heteroarylkyl refers to an alkyl group substituted by a heteroaryl, wherein the alkyl and heteroaryl portions independently are optionally substituted.
• heterocycle As used herein, the terms “heterocycle,” “heterocyclyl,” “heterocyclic radical,” and “heterocyclic ring” are used interchangeably and refer to a stable 5- to 7-membered monocyclic or 7-10-membered bicyclic heterocyclic moiety that is either saturated or partially unsaturated, and having, in addition to carbon atoms, one or more, preferably one to four, heteroatoms, as defined above.
• nitrogen includes a substituted nitrogen.
• the nitrogen may be N (as in 3,4-dihydro- 2H-pyrrolyl), H (as in pyrrolidinyl), or + R (as in N-substituted pyrrolidinyl).
• a heterocyclic ring can be attached to its pendant group at any heteroatom or carbon atom that results in a stable structure and any of the ring atoms can be optionally substituted.
• saturated or partially unsaturated heterocyclic radicals include, without limitation, tetrahydrofuranyl, tetrahydrothiophenyl pyrrolidinyl, piperidinyl, pyrrolinyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl, decahydroquinolinyl, oxazolidinyl, piperazinyl, dioxanyl, dioxolanyl, diazepinyl, oxazepinyl, thiazepinyl, morpholinyl, and quinuclidinyl.
• heterocycle refers to an alkyl group substituted by a heterocyclyl, wherein the alkyl and heterocyclyl portions independently are optionally substituted.
• partially unsaturated refers to a ring moiety that includes at least one double or triple bond.
• partially unsaturated is intended to encompass rings having multiple sites of unsaturation, but is not intended to include aryl or heteroaryl moieties, as herein defined.
• compounds of the invention may contain "optionally substituted” moieties.
• substituted whether preceded by the term “optionally” or not, means that one or more hydrogens of the designated moiety are replaced with a suitable substituent.
• an "optionally substituted” group may have a suitable substituent at each substitutable position of the group, and when more than one position in any given structure may be substituted with more than one substituent selected from a specified group, the substituent may be either the same or different at every position.
• Combinations of substituents envisioned by this invention are preferably those that result in the formation of stable or chemically feasible compounds.
• stable refers to compounds that are not substantially altered when subjected to conditions to allow for their production, detection, and, in certain embodiments, their recovery, purification, and use for one or more of the purposes disclosed herein.
• Suitable monovalent substituents on R° are independently halogen, -(CH 2 )o- 2 R*, -(haloR"), -(CH 2 )o- 2 OH, -(CH 2 ) 0 - 2 OR", -(CH 2 ) 0 - 2 CH(OR") 2 ; -O(haloR'), -CN, -N 3 , -(CH 2 ) 0 - 2 C(0)R', -(CH 2 )o- 2 C(0)OH, -(CH 2 )o- 2 C(0)OR', -(CH 2 ) 0 - 2 SR', -(CH 2 ) 0 - 2 SH, -(CH 2 ) 0 - 2 NH 2 , - (CH 2 )o- 2 NHR e , -(CH 2 )o- 2 NR' 2
• Suitable divalent substituents that are bound to vicinal substitutable carbons of an "optionally substituted” group include: -0(CR * 2 ) 2 - 3O-, wherein each independent occurrence of R * is selected from hydrogen, Ci-6 aliphatic which may be substituted as defined below, or an unsubstituted 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• Suitable substituents on the aliphatic group of R * include halogen, -R", -(haloR"), -OH, -OR', -O(haloR'), -CN, -C(0)OH, -C(0)OR', - H 2 , -NHR", -NR' 2 , or -NO2, wherein each R" is unsubstituted or where preceded by "halo” is substituted only with one or more halogens, and is independently C1-4 aliphatic, -CH 2 Ph, -0(CH 2 )o-iPh, or a 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• Suitable substituents on a substitutable nitrogen of an "optionally substituted" group include -R ⁇ , - R ⁇ 2 , -C(0)R ⁇ , -C(0)OR ⁇ , -C(0)C(0)R ⁇ , C(0)CH 2 C(0)R ⁇ , -S(0) 2 R ⁇ , -S(0) 2 R ⁇ 2 , -C(S) R ⁇ 2 , -C( H) R ⁇ 2 , or -N(R ⁇ )S(0) 2 R ⁇ ; wherein each R ⁇ is independently hydrogen, Ci-6 aliphatic which may be substituted as defined below, unsubstituted -OPh, or an unsubstituted 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or, notwithstanding the definition above, two independent occurrences of R ⁇ , taken together with their intervening atom(s) form
• Suitable substituents on the aliphatic group of R ⁇ are independently halogen, - R', -(haloR*), -OH, -OR', -O(haloR'), -CN, -C(0)OH, -C(0)OR', -NH 2 , -NHR', -NR' 2 , or -NO2, wherein each R* is unsubstituted or where preceded by "halo" is substituted only with one or more halogens, and is independently C1-4 aliphatic, -CH 2 Ph, -0(CH 2 )o-iPh, or a 5-6- membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• the term "pharmaceutically acceptable salt” refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio.
• Pharmaceutically acceptable salts are well known in the art. For example, S. M. Berge et al., describe pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 1977, 66, 1-19, incorporated herein by reference.
• Pharmaceutically acceptable salts of the compounds of this invention include those derived from suitable inorganic and organic acids and bases.
• Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange.
• inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid
• organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange.
• salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphor sulfonate, citrate, cyclopentanepropionate, digluconate, dodecyl sulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2- hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pec
• Salts derived from appropriate bases include alkali metal, alkaline earth metal, ammonium and salts.
• Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like.
• Further pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, loweralkyl sulfonate and aryl sulfonate.
• structures depicted herein are also meant to include all isomeric (e.g., enantiomeric, diastereomeric, and geometric (or conformational)) forms of the structure; for example, the R and S configurations for each asymmetric center, Z and E double bond isomers, and Z and E conformational isomers. Therefore, single stereochemical isomers as well as enantiomeric, diastereomeric, and geometric (or conformational) mixtures of the present compounds are within the scope of the invention. Unless otherwise stated, all tautomeric forms of the compounds of the invention are within the scope of the invention.
• structures depicted herein are also meant to include compounds that differ only in the presence of one or more isotopically enriched atoms.
• compounds having the present structures including the replacement of hydrogen by deuterium or tritium, or the replacement of a carbon by a 13 C- or 14 C-enriched carbon are within the scope of this invention.
• Such compounds are useful, for example, as analytical tools, as probes in biological assays, or as therapeutic agents in accordance with the present invention.
• a warhead moiety, R 1 of a provided compound comprises one or more deuterium atoms.
• an inhibitor is defined as a compound that binds to and /or inhibits PHGDH with measurable affinity.
• an inhibitor has an IC50 and/or binding constant of less than about 100 ⁇ , less than about 50 ⁇ , less than about 1 ⁇ , less than about 500 nM, less than about 100 nM, less than about 10 nM, or less than about 1 nM.
• measurable affinity and “measurably inhibit,” as used herein, means a measurable change in PHGDH activity between a sample comprising a compound of the present invention, or composition thereof, and PHGDH, and an equivalent sample comprising PHGDH, in the absence of said compound, or composition thereof.
• the present invention provides a compound of formula I:
• R 1 is hydrogen or C1-4 alkyl; each of R 2 and R 3 is independently halogen, -OR, -CN, Ci-6 aliphatic optionally substituted with 1, 2, or 3 halogens, or -L-R'; or R 2 and R 3 are optionally taken together with the carbon atoms to which they are attached and any intervening atoms to form a 5-8 membered partially unsaturated ring having 0-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
• R 4 is hydrogen, halogen, -OR 5 , -CN, Ci-6 aliphatic optionally substituted with 1, 2, or 3 halogens, or -L-R';
• each R is independently hydrogen or an optionally substituted group selected from Ci-6 aliphatic, a 3-8 membered saturated or partially unsaturated monocyclic carbocyclic ring, phenyl, an 8-10 membered bicyclic aromatic carbocyclic ring, a 4-8 membered saturated or partially unsaturated monocyclic heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur, a 5-6 membered monocyclic heteroaromatic ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or an 8-10 membered bicyclic heteroaromatic ring having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
• R 5 is hydrogen, -(CH2) n -phenyl, or Ci-6 alkyl optionally substituted with 1, 2, or 3 halogens; each L is independently a Ci-6 bivalent straight or branched hydrocarbon chain wherein 1-4 methylene units of the chain are independently and optionally replaced with -0-, -C(O)-, - C(0)0-, -OC(O)-, -OC(0)N(R)-, -(R)NC(0)0-, -C(0)N(R)-, -(R)NC(O)-, -N(R)-, - N(R)C(0)N(R)-, -S-, -SO-, or -S0 2 -;
• each R' is independently hydrogen, Ci-6 aliphatic, or an optionally substituted 4-8 membered saturated or partially unsaturated monocyclic heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
• R 6 is hydrogen or Ci-4 alkyl
• R 7 is hydrogen, -C0 2 R, Ci- 6 optionally substituted aliphatic group, or a bivalent 3-7 membered ring;
• L 1 is a covalent bond or a Ci -8 bivalent straight or branched hydrocarbon chain wherein 1-5 methylene units of the chain are independently and optionally replaced with -0-, -C(O)-, - C(0)0-, -OC(O)-, -N(R)-, -C(0)N(R)-, -(R)NC(O)-, -OC(0)N(R)-, -(R)NC(0)0-, - N(R)C(0)N(R)-, -S-, -SO-, -SO2-, -S0 2 N(R)-, -(R)NS0 2 -, -C(S)-, -C(S)0-, -OC(S)-, - C(S)N(R)-, -(R)NC(S)-, -(R)NC(S)N(R)-, or -Cy-; each -Cy- is independently a bivalent 6-membered ary
• R 8 is hydrogen, -CO2R, or Ci-6 optionally substituted aliphatic;
• R 9 is hydrogen, halogen, C1-4 alkyl, -CN, -OR, -(CH2) n -(optionally substituted phenyl), or L 2 -R 8 ; each L 2 is independently Ci -8 bivalent straight or branched hydrocarbon chain wherein 1-4 methylene units of the chain are independently and optionally replaced with -0-, -C(O)-, - C(0)0-, -OC(O)-, -N(R)-, -C(0)N(R)-, -(R)NC(O)-, -OC(0)N(R)-, -(R)NC(0)0-, - N(R)C(0)N(R)-, -S-, -SO-, -SO2-, -S0 2 N(R)-, -(R)NS0 2 -, -C(S)-, -C(S)0-, -OC(S)-, - C(S)N(R)-, -(R)NC
• n is independently 0, 1, 2, 3, 4, or 5.
• the present invention provides a compound of formula I wherein said compound is other than compound 1-39 or 1-41.
• R 1 is hydrogen or C1-4 alkyl. In some embodiments, R 1 is hydrogen. In some embodiments, Rl is C1-4 alkyl. In some embodiments, R 1 is methyl. In some embodiments, R 1 is ethyl.
• R 1 is selected from those depicted in Table 1, below.
• R 2 is independently halogen, -OR, -CN, Ci- 6 aliphatic optionally substituted with 1 , 2, or 3 halogens, or -L-R' ; or R 2 and R 3 are optionally taken together with the carbon atoms to which they are attached and any intervening atoms to form a 5- 8 membered partially unsaturated ring having 0-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• R 2 is halogen. In some embodiments, R 2 is -OR. In some embodiments, R 2 is -CN. In some embodiments, R 2 is Ci-6 aliphatic optionally substituted with 1, 2, or 3, halogens. In some embodiments, R 2 is -L-R'.
• R 2 and R 3 are taken together with the carbon atoms to which they are attached and any intervening atoms to form a 5-8 membered partially unsaturated ring having 0-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur [0043]
• R 2 is F, CI, -CF 3 , -OCF 3 , -OCHF 2 , -OCH 2 Ph, -OCH 3 , -CN,
• R 2 is F or CI. In some embodiments, R 2 is -OCH 3 . In some embodiments, R 2 is -CH 3 .
• R 2 is selected from those depicted in Table 1, below.
• R 3 is independently halogen, -OR, -CN, Ci- 6 aliphatic optionally substituted with 1 , 2, or 3 halogens, or -L-R' ; or R 2 and R 3 are optionally taken together with the carbon atoms to which they are attached and any intervening atoms to form a 5-
• R 3 is halogen. In some embodiments, R 3 is -OR. In some embodiments, R 3 is -CN. In some embodiments, R 3 is Ci-6 aliphatic optionally substituted with 1, 2, or 3, halogens. In some embodiments, R 3 is -L-R'. In some embodiments, R 2 and R 3 are taken together with the carbon atoms to which they are attached and any intervening atoms to form a 5-8 membered partially unsaturated ring having 0-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• R 3 is F, CI, -CF 3 , -OCF 3 , -OCHF 2 , -OCH 2 Ph, -OCH 3 , -CN, -
• R 3 is F or CI. In some embodiments, R 3 is -OCH 3 . In some embodiments, R 3 is -CH 3 .
• R 3 is selected from those depicted in Table 1, below.
• R 4 is hydrogen, halogen, -OR 5 , -CN, Ci- 6 aliphatic optionally substituted with 1, 2, or 3 halogens, or -L-R'.
• R 4 is hydrogen. In some embodiments, R 4 is halogen. In some embodiments, R 4 is -OR 5 . In some embodiments, R 4 is Ci-6 aliphatic optionally substituted with 1, 2, or 3 halogens. In some embodiments, R 4 is -L-R'. 0051] In some embodiments, R 4 is F, CI, -CF 3 , -OCF 3 , -OCHF 2 , -OCH 2 Ph, -OCH 3 , -CN, - In some embodiments, R 4 is F or CI. In some embodiments, R 4 is -OCH 3 . In some embodiments, R 4 is -CH 3 .
• R 4 is selected from those depicted in Table 1, below.
• each R is independently hydrogen or an optionally substituted group selected from Ci- 6 aliphatic, a 3-8 membered saturated or partially unsaturated monocyclic carbocyclic ring, phenyl, an 8-10 membered bicyclic aromatic carbocyclic ring, a 4-8 membered saturated or partially unsaturated monocyclic heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur, a 5-6 membered monocyclic heteroaromatic ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or an 8-10 membered bicyclic heteroaromatic ring having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• R is hydrogen. In some embodiments, R is an optionally substituted Ci- 6 aliphatic group. In some embodiments, R is an optionally substituted 3-8 membered saturated or partially unsaturated monocyclic carbocyclic ring. In some embodiments, R is an optionally substituted phenyl. In some embodiments, R is an optionally substituted 8-10 membered bicyclic aromatic carbocyclic ring. In some embodiments, R is an optionally substituted 4-8 membered saturated or partially unsaturated monocyclic heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• R is a 5-6 membered monocyclic heteroaromatic ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments, R is an optionally substituted 8- 10 membered bicyclic heteroaromatic ring having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• Ris selected from those depicted in Table 1, below.
• each L is independently a Ci- 6 bivalent straight or branched hydrocarbon chain wherein 1-4 methylene units of the chain are independently and optionally replaced with -0-, -C(O)-, -C(0)0-, -OC(O)-, -OC(0)N(R)-, -(R)NC(0)0-, - C(0)N(R)-, -(R)NC(O)-, -N(R)-, -N(R)C(0)N(R)-, -S-, -SO-, or -S0 2 -.
• L is a Ci-6 bivalent straight or branched hydrocarbon chain wherein 1-4 methylene units of the chain are independently replaced with -0-, -C(O)-, -C(0)0-, - OC(O)-, -OC(0)N(R)-, -(R)NC(0)0-, -C(0)N(R)-, -(R)NC(O)-, -N(R)-, -N(R)C(0)N(R)-, -S-, - SO-, or -SO2-.
• 1 or 2 methylene units of the chain are replaced with -0-.
• 1 or 2 methylene units of the chain are replaced with -C(O)-. In some embodiments, 1 or 2 methylene units of the chain are replaced with -C(0)0-. In some embodiments, 1 or 2 methylene units of the chain are replaced with -C(0)0-. In some embodiments, 1 or 2 methylene units of the chain are replaced with -OC(O)-. In some embodiments, 1 or 2 methylene units of the chain are replaced with -OC(0)N(R)-. In some embodiments, 1 or 2 methylene units of the chain are replaced with -(R)NC(0)0-. In some embodiments, 1 or 2 methylene units are replaced with -C(0)N(R)-.
• 1 or 2 methylene units of the chain are replaced with -(R)NC(O)-. In some embodiments, 1 or 2 methylene units of the chain are replaced with -N(R)-. In some embodiments, 1 or 2 methylene units of the chain are replaced with -N(R)C(0)N(R)-. In some embodiments, 1 or 2 methylene units of the chain are replaced with -S-. In some embodiments, 1 or 2 methylene units of the chain are replaced with -SO-. In some embodiments, 1 or 2 methylene units are replaced with-S0 2 -.
• 1 or 2 methylene units of the chain are independently replaced with -0-, - C(O)-, -C(0)0-, -OC(O)-, -OC(0)N(R)-, -(R)NC(0)0-, -C(0)N(R)-, -(R)NC(O)-, -N(R)-, - N(R)C(0)N(R)-, -S-, -SO-, or -SO2-, wherein each R is independently hydrogen or methyl.
• L is a Ci-6 bivalent straight or branched hydrocarbon chain wherein 1, 2, or 3 methylene units of the chain are independently replaced with -0-, -C(O)-, - C(0)0-, -OC(O)-, -OC(0)N(R)-, -(R)NC(0)0-, -C(0)N(R)-, -(R)NC(O)-, -N(R)-, or - N(R)C(0)N(R)-, wherein R is hydrogen or methyl.
• L is a Ci-6 bivalent straight or branched hydrocarbon chain wherein 1 or 2 methylene units of the chain are independently replaced with -0-, -C(O)-, -C(0)N(R)-, -(R)NC(O)-, or -N(R)-, wherein each R is independently hydrogen or methyl.
• L is -0-. In some embodiments, L is -O-CH2-. In some embodiments, L is -0-CH2-C(0)NH-.
• L is selected from those depicted in Table 1, below.
• each R' is independently hydrogen, Ci- 6 aliphatic, or an optionally substituted 4-8 membered saturated or partially unsaturated monocyclic heterocyclic ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• R' is hydrogen. In some embodiments, R' is Ci-6 aliphatic. In some embodiments, R' is an optionally substituted 4-8 membered saturated or partially saturated monocyclic heterocyclic ring having 1-3 heteroatoms independently selected from nitrogen, oxygen or sulfur.
• R' is . In some embodiments, R' is
• R' is
• R' is selected from those depicted in Table 1, below.
• R 5 is hydrogen, -(CH2) n -phenyl, or Ci-6 alkyl optionally substituted with 1, 2, or 3 halogens.
• R 5 is hydrogen. In some embodiments, R 5 is -(CH2) n -phenyl. In some embodiments, R 5 is Ci-6 alkyl optionally substituted with 1, 2, or 3 halogens.
• R 5 is selected from those depicted in Table 1, below.
• R 6 is hydrogen or Ci-4 alkyl. In some embodiments, R 6 is hydrogen. In some embodiments, R 6 is Ci-4 alkyl.
• R 6 is methyl
• R 6 is selected from those depicted in Table 1, below.
• R 7 is hydrogen, -CO2R, optionally substituted
• Ci-6 aliphatic or a bivalent 3-7 membered ring.
• R 7 is hydrogen. In some embodiments, R 7 is -CO2R. In some embodiments, R 7 is Ci-6 aliphatic. In some embodiments, R 7 is a bivalent 3-7 membered ring. [0073] In some embodiments, R is hydrogen, methyl, ethyl, cyclopropyl, cyclobutyl, or - C0 2 H.
• R 7 is selected from those depicted in Table 1, below.
• L 1 is a covalent bond or a Ci -8 bivalent straight or branched hydrocarbon chain wherein 1-5 methylene units of the chain are independently and optionally replaced with -0-, -C(O)-, -C(0)0-, -OC(O)-, -N(R)-, -C(0)N(R)-, -(R)NC(O)-, - OC(0)N(R)-, -(R)NC(0)0-, -N(R)C(0)N(R)-, -S-, -SO-, -SO2-, -S0 2 N(R)-, -(R)NS0 2 -, -C(S)-, - C(S)0-, -OC(S)-, -C(S)N(R)-, -(R)NC(S)-, -(R)NC(S)N(R)-, or -Cy-.
• L 1 is a covalent bond. In some embodiments, L 1 is a Ci -8 bivalent straight or branched hydrocarbon chain. In some embodiments, L 1 is a Ci -8 bivalent straight or branched hydrocarbon chain wherein 1-5 methylene units of the chain are independently replaced with -0-, -C(O)-, -C(0)0-, -OC(O)-, -N(R)-, -C(0)N(R)-, -(R)NC(O)-, -OC(0)N(R)-, - (R)NC(0)0-, -N(R)C(0)N(R)-, -S-, -SO-, -SO2-, -S0 2 N(R)-, -(R)NS0 2 -, -C(S)-, -C(S)0-, - OC(S)-, -C(S)N(R)-, -(R)NC(S)-, -, -
• 1, 2, or 3 methylene units of the chain are replaced with -0-. In some embodiments, 1, 2, or 3 methylene units of the chain are replaced with -C(O)-. In some embodiments, 1, 2, or 3 methylene units of the chain are replaced with -C(0)0- or -OC(O)-. In some embodiments, 1, 2, or 3 methylene units of the chain are replaced with -N(R)-. In some embodiments, 1, 2, or 3 methylene units of the chain are replaced with -C(0)N(R)- or -(R)NC(O)-. In some embodiments, 1, 2, or 3 methylene units of the chain are replaced with -OC(0)N(R)- or -(R)NC(0)0-.
• 1, 2, or 3 methylene units of the chain are replaced with -N(R)C(0)N(R)-. In some embodiments, 1, 2, or 3 methylene units of the chain are replaced with -S-. In some embodiments, 1, 2, or 3 methylene units of the chain are replaced with -SO-. In some embodiments, 1, 2, or 3 methylene units of the chain are replaced with -SO2-. In some embodiments, 1, 2, or 3 methylene units of the chain are replaced with -S0 2 N(R)- or -(R)NS0 2 -. In some embodiments, 1, 2, or 3 methylene units of the chain are replaced with -C(S)-.
• 1, 2, or 3 methylene units of the chain are replaced with -C(S)0- or -OC(S)-. In some embodiments, 1, 2, or 3 methylene units of the chain are replaced with -C(S)N(R)- or -(R)NC(S)-. In some embodiments, 1, 2, or 3 methylene units of the chain are replaced with -(R)NC(S)N(R)-. In some embodiments, 1, 2, or 3 methylene units of the chain are replaced with -Cy-.
• L 1 is a Ci- 6 bivalent straight or branched hydrocarbon chain wherein 1, 2, or 3 methylene units of the chain are independently and optionally replaced with -0-, -C(0)-, -C(0)0-, -0C(0)-, -N(R)-, -C(0)N(R)-, -(R)NC(O)-, -OC(0)N(R)-, - (R)NC(0)0-, -N(R)C(0)N(R)-, -S-, -SO-, -SO2-, -S0 2 N(R)-, -(R)NS0 2 -, -C(S)-, -C(S)0-, - OC(S)-, -C(S)N(R)-, -(R)NC(S)-, -(R)NC(S)N(R)-, or -Cy-, wherein each R is independently hydrogen, -CH 2 -phenyl, phen
• L 1 is a Ci-6 bivalent straight or branched hydrocarbon chain wherein 1, 2, 3, or 4 methylene units of the chain are independently and optionally replaced with -0-, -C(O)-, -C(0)0-, -OC(O)-, - H-, -C(0) H-, - HC(O)-, - HC(0) H-, -SO2-, or -Cy-.
• L 1 is a Ci-6 bivalent straight or branched hydrocarbon chain wherein 1, 2, or 3 methylene units of the chain are independently replaced with -0-, -C(O)-, - C(0)0-, -OC(O)-, - H-, -C(0) H-, - HC(O)-, - HC(0) H-, -SO2-, or -Cy-.
• L 1 is a Ci-6 bivalent straight or branched hydrocarbon chain wherein 1, 2, or 3 methylene units of the chain are independently replaced with -C(O)-, - H-, - C(0) H-, - HC(O)-, - HC(0) H-, -SO2-, or -Cy-.
• L 1 is a C3-6 bivalent straight or branched hydrocarbon chain wherein 2 or 3 methylene units of the chain are independently replaced with -SO2-, -SO2 H-, - C(0)0-, -C(0) H-, or - HC(0) H-.
• L 1 is a C3-6 bivalent branched hydrocarbon chain wherein 2 or 3 methylene units of the chain are independently replaced with - SO2-, -SO2 H-, -C(0)0-, -C(0) H-, or - HC(0) H-.
• the methylene unit of L 1 is replaced with -SO2-. In some embodiments, the methylene unit of L 1 is replaced with -SO2 H-. In some embodiments, the methylene unit of L 1 is substituted with two methyl groups. In some embodiments, the methylene unit of L 1 is replaced with -C(0) H-. In some embodiments, the methylene unit of L 1 is replaced with -SO2- and the adjacent methylene unit is replaced with -NHC(0)NH-. In some embodiments, the methylene unit of L 1 is substituted with two methyl groups and the adjacent methylene unit is replaced with - HC(0)NH-.
• L 1 is a covalent bond.
• L 1 In some embodiments, In some embodiment In some
• L 1 In some embodiments, L 1 In some embodiments, L 1 In some
• L 1 In some embodiments, L 1 In some
• L 1 In some embodiments, L is In some embodiments, L is In some
• L 1 is In some embodiments, L 1 is In some
• L 1 is .
• L 1 is selected from those depicted in Table 1, below.
• each -Cy- is independently a bivalent 6- membered arylene ring containing 0-2 nitrogen atoms wherein the ring is optionally substituted with 1 or 2 substituents independently selected from C 1-4 alkyl or -OR, or a bivalent 5-membered heteroarylene ring with 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a bivalent partially unsaturated 8-10 membered bicyclic heterocyclene ring with 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• -Cy- is a bivalent 6-membered arylene ring containing 0-2 nitrogen atoms.
• -Cy- is a bivalent 6-membered arylene ring containing 0- 2 nitrogen atoms wherein the ring is substituted with 1 or 2 substituents independently selected from Ci-4 alkyl or -OR, wherein R is hydrogen or C 1-4 alkyl.
• -Cy- is a bivalent 5-membered heteroarylene ring with 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• -Cy- is a bivalent partially unsaturated 8-10 membered bicyclic heterocyclene ring with 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• -Cy- is selected from those depicted in Table 1, below.
• the present invention provides a compound of Formulae Il-a, Il-b, II-c, Il-d, Il-e, Il-f, or Il-g:
• R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , R, R , L 1 , -Cy-, and n is defined above and described in embodiments herein.
• the present invention provides a compound depicted in Table 1, above, or a pharmaceutically acceptable salt thereof.
• compositions are provided.
• the invention provides a composition comprising a compound of this invention or a pharmaceutically acceptable derivative thereof and a pharmaceutically acceptable carrier, adjuvant, or vehicle.
• the amount of compound in compositions of this invention is such that is effective to measurably inhibit PHGDH, or a mutant thereof, in a biological sample or in a patient.
• the amount of compound in compositions of this invention is such that is effective to measurably inhibit PHGDH, or a mutant thereof, in a biological sample or in a patient.
• a composition of this invention is formulated for administration to a patient in need of such composition.
• a composition of this invention is formulated for oral administration to a patient.
• patient means an animal, preferably a mammal, and most preferably a human.
• compositions of this invention refers to a nontoxic carrier, adjuvant, or vehicle that does not destroy the pharmacological activity of the compound with which it is formulated.
• Pharmaceutically acceptable carriers, adjuvants or vehicles that may be used in the compositions of this invention include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins, such as human serum albumin, buffer substances such as phosphates, glycine, sorbic acid, potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, cellulose-based substances, polyethylene glycol, sodium carboxymethylcellulose, polyacrylates, waxes, polyethylene- polyoxypropylene
• a "pharmaceutically acceptable derivative” means any non-toxic salt, ester, salt of an ester or other derivative of a compound of this invention that, upon administration to a recipient, is capable of providing, either directly or indirectly, a compound of this invention or an inhibitorily active metabolite or residue thereof.
• the term "inhibitorily active metabolite or residue thereof means that a metabolite or residue thereof is also an inhibitor of PHGDH, or a mutant thereof.
• compositions of the present invention may be administered orally, parenterally, by inhalation spray, topically, rectally, nasally, buccally, vaginally or via an implanted reservoir.
• parenteral as used herein includes subcutaneous, intravenous, intramuscular, intra-articular, intra-synovial, intrasternal, intrathecal, intrahepatic, intralesional and intracranial injection or infusion techniques.
• the compositions are administered orally, intraperitoneally or intravenously.
• Sterile injectable forms of the compositions of this invention may be aqueous or oleaginous suspension. These suspensions may be formulated according to techniques known in the art using suitable dispersing or wetting agents and suspending agents.
• the sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally acceptable diluent or solvent, for example as a solution in 1,3-butanediol.
• a non-toxic parenterally acceptable diluent or solvent for example as a solution in 1,3-butanediol.
• acceptable vehicles and solvents that may be employed are water, Ringer's solution and isotonic sodium chloride solution.
• sterile, fixed oils are conventionally employed as a solvent or suspending medium.
• any bland fixed oil may be employed including synthetic mono- or di-glycerides.
• Fatty acids such as oleic acid and its glyceride derivatives are useful in the preparation of injectables, as are natural pharmaceutically-acceptable oils, such as olive oil or castor oil, especially in their polyoxy ethyl ated versions.
• These oil solutions or suspensions may also contain a long-chain alcohol diluent or dispersant, such as carboxymethyl cellulose or similar dispersing agents that are commonly used in the formulation of pharmaceutically acceptable dosage forms including emulsions and suspensions.
• Other commonly used surfactants such as Tweens, Spans and other emulsifying agents or bioavailability enhancers which are commonly used in the manufacture of pharmaceutically acceptable solid, liquid, or other dosage forms may also be used for the purposes of formulation.
• compositions of this invention may be orally administered in any orally acceptable dosage form including, but not limited to, capsules, tablets, aqueous suspensions or solutions.
• carriers commonly used include lactose and corn starch.
• Lubricating agents such as magnesium stearate, are also typically added.
• useful diluents include lactose and dried cornstarch.
• aqueous suspensions are required for oral use, the active ingredient is combined with emulsifying and suspending agents. If desired, certain sweetening, flavoring or coloring agents may also be added.
• compositions of this invention may be administered in the form of suppositories for rectal administration.
• suppositories for rectal administration.
• suppositories can be prepared by mixing the agent with a suitable non-irritating excipient that is solid at room temperature but liquid at rectal temperature and therefore will melt in the rectum to release the drug.
• suitable non-irritating excipient include cocoa butter, beeswax and polyethylene glycols.
• compositions of this invention may also be administered topically, especially when the target of treatment includes areas or organs readily accessible by topical application, including diseases of the eye, the skin, or the lower intestinal tract. Suitable topical formulations are readily prepared for each of these areas or organs.
• Topical application for the lower intestinal tract can be effected in a rectal suppository formulation (see above) or in a suitable enema formulation. Topically-transdermal patches may also be used.
• compositions may be formulated in a suitable ointment containing the active component suspended or dissolved in one or more carriers.
• Carriers for topical administration of compounds of this invention include, but are not limited to, mineral oil, liquid petrolatum, white petrolatum, propylene glycol, polyoxyethylene, polyoxypropylene compound, emulsifying wax and water.
• provided pharmaceutically acceptable compositions can be formulated in a suitable lotion or cream containing the active components suspended or dissolved in one or more pharmaceutically acceptable carriers.
• Suitable carriers include, but are not limited to, mineral oil, sorbitan monostearate, polysorbate 60, cetyl esters wax, cetearyl alcohol, 2-octyldodecanol, benzyl alcohol and water.
• compositions may be formulated as micronized suspensions in isotonic, pH adjusted sterile saline, or, preferably, as solutions in isotonic, pH adjusted sterile saline, either with or without a preservative such as benzylalkonium chloride.
• the pharmaceutically acceptable compositions may be formulated in an ointment such as petrolatum.
• compositions of this invention may also be administered by nasal aerosol or inhalation.
• Such compositions are prepared according to techniques well- known in the art of pharmaceutical formulation and may be prepared as solutions in saline, employing benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, fluorocarbons, and/or other conventional solubilizing or dispersing agents.
• compositions of this invention are formulated for oral administration. Such formulations may be administered with or without food. In some embodiments, pharmaceutically acceptable compositions of this invention are administered without food. In other embodiments, pharmaceutically acceptable compositions of this invention are administered with food.
• compositions of the present invention that may be combined with the carrier materials to produce a composition in a single dosage form will vary depending upon the host treated, the particular mode of administration.
• provided compositions should be formulated so that a dosage of between 0.01 - 100 mg/kg body weight/day of the inhibitor can be administered to a patient receiving these compositions.
• a specific dosage and treatment regimen for any particular patient will depend upon a variety of factors, including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, rate of excretion, drug combination, and the judgment of the treating physician and the severity of the particular disease being treated.
• the amount of a compound of the present invention in the composition will also depend upon the particular compound in the composition.
• the activity of a compound utilized in this invention as an inhibitor of PHGDH, or a mutant thereof may be assayed in vitro, in vivo or in a cell line.
• In vitro assays include assays that determine inhibition of PHGDH, or a mutant thereof. Alternate in vitro assays quantitate the ability of the inhibitor to bind to PHGDH.
• Detailed conditions for assaying a compound utilized in this invention as an inhibitor of PHGDH, or a mutant thereof, are set forth in the Examples below.
• treatment refers to reversing, alleviating, delaying the onset of, or inhibiting the progress of a disease or disorder, or one or more symptoms thereof, as described herein.
• treatment may be administered after one or more symptoms have developed.
• treatment may be administered in the absence of symptoms.
• treatment may be administered to a susceptible individual prior to the onset of symptoms (e.g., in light of a history of symptoms and/or in light of genetic or other susceptibility factors). Treatment may also be continued after symptoms have resolved, for example to prevent or delay their recurrence.
• the present invention provides a method for treating a PHGDH-mediated disorder comprising the step of administering to a patient in need thereof a compound of the present invention, or pharmaceutically acceptable composition thereof.
• PHGDH-mediated disorders, diseases, and/or conditions as used herein means any disease or other deleterious condition in which PHGDH, or a mutant thereof, is known to play a role. Accordingly, another embodiment of the present invention relates to treating or lessening the severity of one or more diseases in which PHGDH, or a mutant thereof, are known to play a role.
• the present invention provides a method for treating one or more disorders, diseases, and/or conditions wherein the disorder, disease, or condition includes, but is not limited to, a cellular proliferative disorder.
• the present invention features methods and compositions for the diagnosis and prognosis of cellular proliferative disorders (e.g., cancer) and the treatment of these disorders by targeting PHGDH of the serine biosynthetic pathway.
• cellular proliferative disorders described herein include, e.g., cancer, obesity, and proliferation-dependent diseases. Such disorders may be diagnosed using methods known in the art.
• Cancer includes, in one embodiment, without limitation, leukemias (e.g., acute leukemia, acute lymphocytic leukemia, acute myelocytic leukemia, acute myeloblastic leukemia, acute promyelocytic leukemia, acute myelomonocytic leukemia, acute monocytic leukemia, acute erythroleukemia, chronic leukemia, chronic myelocytic leukemia, chronic lymphocytic leukemia), polycythemia vera, lymphoma (e.g., Hodgkin's disease or non-Hodgkin's disease), Waldenstrom's macroglobulinemia, multiple myeloma, heavy chain disease, and solid tumors such as sarcomas and carcinomas (e.g., fibrosarcoma, myxosarcoma, liposarcoma, chondrosarcoma, osteogenic sarcoma, chordoma, angiosarcoma
• Cancers includes, in another embodiment, without limitation, mesothelioma, hepatobilliary (hepatic and billiary duct), bone cancer, pancreatic cancer, skin cancer, cancer of the head or neck, cutaneous or intraocular melanoma, ovarian cancer, colon cancer, rectal cancer, cancer of the anal region, stomach cancer, gastrointestinal (gastric, colorectal, and duodenal), uterine cancer, carcinoma of the fallopian tubes, carcinoma of the endometrium, carcinoma of the cervix, carcinoma of the vagina, carcinoma of the vulva, Hodgkin's Disease, cancer of the esophagus, cancer of the small intestine, cancer of the endocrine system, cancer of the thyroid gland, cancer of the parathyroid gland, cancer of the adrenal gland, sarcoma of soft tissue, cancer of the urethra, cancer of the penis, prostate cancer, testicular cancer, chronic or acute leukemia, chronic myeloid leukemia,
• the present invention provides a method for treating a tumor in a patient in need thereof, comprising administering to the patient any of the compounds, salts or pharmaceutical compositions described herein.
• the tumor comprises any of the cancers described herein.
• the tumor comprises melanoma cancer.
• the tumor comprises breast cancer.
• the tumor comprises lung cancer.
• the tumor comprises small cell lung cancer (SCLC).
• the tumor comprises non-small cell lung cancer (NSCLC).
• the tumor is treated by arresting further growth of the tumor.
• the tumor is treated by reducing the size (e.g., volume or mass) of the tumor by at least 5%, 10%, 25%, 50 %, 75%, 90% or 99% relative to the size of the tumor prior to treatment.
• tumors are treated by reducing the quantity of the tumors in the patient by at least 5%, 10%, 25%, 50 %, 75%, 90% or 99% relative to the quantity of tumors prior to treatment.
• proliferative diseases include, e.g., obesity, benign prostatic hyperplasia, psoriasis, abnormal keratinization, lymphoproliferative disorders (e.g., a disorder in which there is abnormal proliferation of cells of the lymphatic system), chronic rheumatoid arthritis, arteriosclerosis, restenosis, and diabetic retinopathy.
• lymphoproliferative disorders e.g., a disorder in which there is abnormal proliferation of cells of the lymphatic system
• chronic rheumatoid arthritis e.g., arteriosclerosis, restenosis, and diabetic retinopathy.
• Proliferative diseases that are hereby incorporated by reference include those described in U.S. Pat. Nos. 5,639,600 and 7,087,648. Inflammatory Disorders and Diseases
• Inflammatory or obstructive airways diseases to which the present invention is applicable include asthma of whatever type or genesis including both intrinsic (non-allergic) asthma and extrinsic (allergic) asthma, mild asthma, moderate asthma, severe asthma, bronchitic asthma, exercise-induced asthma, occupational asthma and asthma induced following bacterial infection.
• Treatment of asthma is also to be understood as embracing treatment of subjects, e.g. of less than 4 or 5 years of age, exhibiting wheezing symptoms and diagnosed or diagnosable as "whez infants", an established patient category of major medical concern and now often identified as incipient or early-phase asthmatics.
• Compounds according to the invention are useful in the treatment of heteroimmune diseases.
• heteroimmune diseases include, but are not limited to, graft versus host disease, transplantation, transfusion, anaphylaxis, allergies (e.g., allergies to plant pollens, latex, drugs, foods, insect poisons, animal hair, animal dander, dust mites, or cockroach calyx), type I hypersensitivity, allergic conjunctivitis, allergic rhinitis, and atopic dermatitis.
• Prophylactic efficacy in the treatment of asthma will be evidenced by reduced frequency or severity of symptomatic attack, e.g. of acute asthmatic or bronchoconstrictor attack, improvement in lung function or improved airways hyperreactivity. It may further be evidenced by reduced requirement for other, symptomatic therapy, such as therapy for or intended to restrict or abort symptomatic attack when it occurs, for example antiinflammatory or bronchodilatory.
• Prophylactic benefit in asthma may in particular be apparent in subjects prone to "morning dipping". "Morning dipping" is a recognized asthmatic syndrome, common to a substantial percentage of asthmatics and characterized by asthma attack, e.g. between the hours of about 4 to 6 am, i.e. at a time normally substantially distant form any previously administered symptomatic asthma therapy.
• Compounds of the current invention can be used for other inflammatory or obstructive airways diseases and conditions to which the present invention is applicable and include acute lung injury (ALI), adult/acute respiratory distress syndrome (ARDS), chronic obstructive pulmonary, airways or lung disease (COPD, COAD or COLD), including chronic bronchitis or dyspnea associated therewith, emphysema, as well as exacerbation of airways hyperreactivity consequent to other drug therapy, in particular other inhaled drug therapy.
• the invention is also applicable to the treatment of bronchitis of whatever type or genesis including, but not limited to, acute, arachidic, catarrhal, croupus, chronic or phthinoid bronchitis.
• pneumoconiosis an inflammatory, commonly occupational, disease of the lungs, frequently accompanied by airways obstruction, whether chronic or acute, and occasioned by repeated inhalation of dusts
• pneumoconiosis an inflammatory, commonly occupational, disease of the lungs, frequently accompanied by airways obstruction, whether chronic or acute, and occasioned by repeated inhalation of dusts
• aluminosis an inflammatory, commonly occupational, disease of the lungs, frequently accompanied by airways obstruction, whether chronic or acute, and occasioned by repeated inhalation of dusts
• aluminosis anthracosis
• asbestosis chalicosis
• ptilosis ptilosis
• siderosis silicosis
• tabacosis tabacosis and byssinosis.
• compounds of the invention are also useful in the treatment of eosinophil related disorders, e.g. eosinophilia, in particular eosinophil related disorders of the airways (e.g.
• eosinophilic infiltration of pulmonary tissues including hypereosinophilia as it effects the airways and/or lungs as well as, for example, eosinophil- related disorders of the airways consequential or concomitant to Loffler's syndrome, eosinophilic pneumonia, parasitic (in particular metazoan) infestation (including tropical eosinophilia), bronchopulmonary aspergillosis, polyarteritis nodosa (including Churg-Strauss syndrome), eosinophilic granuloma and eosinophil-related disorders affecting the airways occasioned by drug-reaction.
• Compounds of the invention are also useful in the treatment of inflammatory or allergic conditions of the skin, for example psoriasis, contact dermatitis, atopic dermatitis, alopecia areata, erythema multiforma, dermatitis herpetiformis, scleroderma, vitiligo, hypersensitivity angiitis, urticaria, bullous pemphigoid, lupus erythematosus, systemic lupus erythematosus, pemphigus vulgaris, pemphigus foliaceus, paraneoplastic pemphigus, epidermolysis bullosa acquisita, acne vulgaris, and other inflammatory or allergic conditions of the skin.
• Compounds of the invention may also be used for the treatment of other diseases or conditions, such as diseases or conditions having an inflammatory component, for example, treatment of diseases and conditions of the eye such as ocular allergy, conjunctivitis, keratoconjunctivitis sicca, and vernal conjunctivitis, diseases affecting the nose including allergic rhinitis, and inflammatory disease in which autoimmune reactions are implicated or having an autoimmune component or etiology, including autoimmune hematological disorders (e.g.
• hemolytic anemia aplastic anemia, pure red cell anemia and idiopathic thrombocytopenia
• systemic lupus erythematosus rheumatoid arthritis, polychondritis, scleroderma, Wegener granulamatosis, dermatomyositis, chronic active hepatitis, myasthenia gravis, Steven-Johnson syndrome, idiopathic sprue, autoimmune inflammatory bowel disease (e.g.
• ulcerative colitis and Crohn's disease irritable bowel syndrome, celiac disease, periodontitis, hyaline membrane disease, kidney disease, glomerular disease, alcoholic liver disease, multiple sclerosis, endocrine opthalmopathy, Grave's disease, sarcoidosis, alveolitis, chronic hypersensitivity pneumonitis, multiple sclerosis, primary biliary cirrhosis, uveitis (anterior and posterior), Sjogren's syndrome, keratoconjunctivitis sicca and vernal keratoconjunctivitis, interstitial lung fibrosis, psoriatic arthritis, systemic juvenile idiopathic arthritis, cryopyrin-associated periodic syndrome, nephritis, vasculitis, diverticulitis, interstitial cystitis, glomerulonephritis (with and without nephrotic syndrome, e.g.
• idiopathic nephrotic syndrome or minal change nephropathy chronic granulomatous disease, endometriosis, leptospiriosis renal disease, glaucoma, retinal disease, ageing, headache, pain, complex regional pain syndrome, cardiac hypertrophy, musclewasting, catabolic disorders, obesity, fetal growth retardation, hyperchlolesterolemia, heart disease, chronic heart failure, mesothelioma, anhidrotic ecodermal dysplasia, Behcet's disease, incontinentia pigmenti, Paget' s disease, pancreatitis, hereditary periodic fever syndrome, asthma (allergic and non-allergic, mild, moderate, severe, bronchitic, and exercise-induced), acute lung injury, acute respiratory distress syndrome, eosinophilia, hypersensitivities, anaphylaxis, nasal sinusitis, ocular allergy, silica induced diseases, COPD (reduction of damage, airways inflammation, bronchial hyperreactivity
• the inflammatory disease which can be treated according to the methods of this invention is an disease of the skin.
• the inflammatory disease of the skin is selected from contact dermatitits, atompic dermatitis, alopecia areata, erythema multiforma, dermatitis herpetiformis, scleroderma, vitiligo, hypersensitivity angiitis, urticaria, bullous pemphigoid, pemphigus vulgaris, pemphigus foliaceus, paraneoplastic pemphigus, epidermolysis bullosa acquisita, and other inflammatory or allergic conditions of the skin.
• the inflammatory disease which can be treated according to the methods of this invention is selected from acute and chronic gout, chronic gouty arthritis, psoriasis, psoriatic arthritis, rheumatoid arthritis, Juvenile rheumatoid arthritis, Systemic juvenile idiopathic arthritis (SJIA), Cryopyrin Associated Periodic Syndrome (CAPS), and osteoarthritis.
• the inflammatory disease which can be treated according to the methods of this invention is a TH17 mediated disease.
• the TH17 mediated disease is selected from Systemic lupus erythematosus, Multiple sclerosis, and inflammatory bowel disease (including Crohn's disease or ulcerative colitis).
• the inflammatory disease which can be treated according to the methods of this invention is selected from Sjogren's syndrome, allergic disorders, osteoarthritis, conditions of the eye such as ocular allergy, conjunctivitis, keratoconjunctivitis sicca and vernal conjunctivitis, and diseases affecting the nose such as allergic rhinitis.
• the invention provides a method of treating a metabolic disease.
• the metabolic disease is selected from Type 1 diabetes, Type 2 diabetes, metabolic syndrome or obesity.
• the compounds and compositions, according to the method of the present invention may be administered using any amount and any route of administration effective for treating or lessening the severity of a cancer, an autoimmune disorder, a proliferative disorder, an inflammatory disorder, a neurodegenerative or neurological disorder, schizophrenia, a bone- related disorder, liver disease, or a cardiac disorder.
• the exact amount required will vary from subject to subject, depending on the species, age, and general condition of the subject, the severity of the infection, the particular agent, its mode of administration, and the like.
• Compounds of the invention are preferably formulated in dosage unit form for ease of administration and uniformity of dosage.
• the expression "dosage unit form" as used herein refers to a physically discrete unit of agent appropriate for the patient to be treated.
• the specific effective dose level for any particular patient or organism will depend upon a variety of factors including the disorder being treated and the severity of the disorder; the activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex and diet of the patient; the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidental with the specific compound employed, and like factors well known in the medical arts.
• patient means an animal, preferably a mammal, and most preferably a human.
• compositions of this invention can be administered to humans and other animals orally, rectally, parenterally, intracisternally, intravaginally, intraperitoneally, topically (as by powders, ointments, or drops), buccally, as an oral or nasal spray, or the like, depending on the severity of the infection being treated.
• the compounds of the invention may be administered orally or parenterally at dosage levels of about 0.01 mg/kg to about 50 mg/kg and preferably from about 1 mg/kg to about 25 mg/kg, of subject body weight per day, one or more times a day, to obtain the desired therapeutic effect.
• Liquid dosage forms for oral administration include, but are not limited to, pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs.
• the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof.
• the oral compositions can also include adj
• Injectable preparations for example, sterile injectable aqueous or oleaginous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents.
• the sterile injectable preparation may also be a sterile injectable solution, suspension or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol.
• the acceptable vehicles and solvents that may be employed are water, Ringer's solution, U.S.P. and isotonic sodium chloride solution.
• sterile, fixed oils are conventionally employed as a solvent or suspending medium.
• any bland fixed oil can be employed including synthetic mono- or diglycerides.
• fatty acids such as oleic acid are used in the preparation of injectables.
• Injectable formulations can be sterilized, for example, by filtration through a bacterial- retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use.
• a compound of the present invention In order to prolong the effect of a compound of the present invention, it is often desirable to slow the absorption of the compound from subcutaneous or intramuscular injection. This may be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of the compound then depends upon its rate of dissolution that, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered compound form is accomplished by dissolving or suspending the compound in an oil vehicle. Injectable depot forms are made by forming microencapsulated matrices of the compound in biodegradable polymers such as polylactide- polyglycolide.
• the rate of compound release can be controlled.
• biodegradable polymers include poly(orthoesters) and poly(anhydrides).
• Depot injectable formulations are also prepared by entrapping the compound in liposomes or microemulsions that are compatible with body tissues.
• compositions for rectal or vaginal administration are preferably suppositories which can be prepared by mixing the compounds of this invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
• suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
• Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules.
• the active compound is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar—agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffin, f) absorption accelerators such as quaternary ammonium compounds, g) wetting agents such as, for example, cetyl alcohol
• Solid compositions of a similar type may also be employed as fillers in soft and hard- filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like.
• the solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical formulating art. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes. Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polethylene glycols and the like.
• the active compounds can also be in micro-encapsulated form with one or more excipients as noted above.
• the solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings, release controlling coatings and other coatings well known in the pharmaceutical formulating art.
• the active compound may be admixed with at least one inert diluent such as sucrose, lactose or starch.
• Such dosage forms may also comprise, as is normal practice, additional substances other than inert diluents, e.g., tableting lubricants and other tableting aids such a magnesium stearate and microcrystalline cellulose.
• the dosage forms may also comprise buffering agents. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner.
• buffering agents include polymeric substances and waxes.
• Dosage forms for topical or transdermal administration of a compound of this invention include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants or patches.
• the active component is admixed under sterile conditions with a pharmaceutically acceptable carrier and any needed preservatives or buffers as may be required.
• Ophthalmic formulation, ear drops, and eye drops are also contemplated as being within the scope of this invention.
• the present invention contemplates the use of transdermal patches, which have the added advantage of providing controlled delivery of a compound to the body.
• Such dosage forms can be made by dissolving or dispensing the compound in the proper medium.
• Absorption enhancers can also be used to increase the flux of the compound across the skin. The rate can be controlled by either providing a rate controlling membrane or by dispersing the compound in a polymer matrix or gel.
• the invention relates to a method of inhibiting PHGDH activity in a biological sample comprising the step of contacting said biological sample with a compound of this invention, or a composition comprising said compound.
• the invention relates to a method of inhibiting PHGDH, or a mutant thereof, activity in a biological sample comprising the step of contacting said biological sample with a compound of this invention, or a composition comprising said compound.
• the invention relates to a method of irreversibly inhibiting PHGDH, or a mutant thereof, activity in a biological sample comprising the step of contacting said biological sample with a compound of this invention, or a composition comprising said compound.
• biological sample includes, without limitation, cell cultures or extracts thereof; biopsied material obtained from a mammal or extracts thereof; and blood, saliva, urine, feces, semen, tears, or other body fluids or extracts thereof.
• Another embodiment of the present invention relates to a method of inhibiting PHGDH in a patient comprising the step of administering to said patient a compound of the present invention, or a composition comprising said compound.
• the invention relates to a method of inhibiting PHGDH, or a mutant thereof, activity in a patient comprising the step of administering to said patient a compound of the present invention, or a composition comprising said compound.
• the invention relates to a method of irreversibly inhibiting PHGDH, or a mutant thereof, activity in a patient comprising the step of administering to said patient a compound of the present invention, or a composition comprising said compound.
• the present invention provides a method for treating a disorder mediated by PHGDH, or a mutant thereof, in a patient in need thereof, comprising the step of administering to said patient a compound according to the present invention or pharmaceutically acceptable composition thereof. Such disorders are described in detail herein.
• additional therapeutic agents that are normally administered to treat that condition may also be present in the compositions of this invention.
• additional therapeutic agents that are normally administered to treat a particular disease, or condition are known as "appropriate for the disease, or condition, being treated.”
• a compound of the current invention may also be used to advantage in combination with other antiproliferative compounds.
• antiproliferative compounds include, but are not limited to aromatase inhibitors; antiestrogens; topoisomerase I inhibitors; topoisomerase II inhibitors; microtubule active compounds; alkylating compounds; histone deacetylase inhibitors; compounds which induce cell differentiation processes; cyclooxygenase inhibitors; MMP inhibitors; mTOR inhibitors; antineoplastic antimetabolites; platin compounds; compounds targeting/decreasing a protein or lipid kinase activity and further anti-angiogenic compounds; compounds which target, decrease or inhibit the activity of a protein or lipid phosphatase; gonadorelin agonists; anti-androgens; methionine aminopeptidase inhibitors; matrix metalloproteinase inhibitors; bisphosphonates; biological response modifiers; antiproliferative antibodies; heparanase inhibitors; inhibitor
• aromatase inhibitor as used herein relates to a compound which inhibits estrogen production, for instance, the conversion of the substrates androstenedione and testosterone to estrone and estradiol, respectively.
• the term includes, but is not limited to steroids, especially atamestane, exemestane and formestane and, in particular, non-steroids, especially aminoglutethimide, roglethimide, pyridoglutethimide, trilostane, testolactone, ketokonazole, vorozole, fadrozole, anastrozole and letrozole.
• Exemestane is marketed under the trade name AromasinTM.
• Formestane is marketed under the trade name LentaronTM.
• Fadrozole is marketed under the trade name AfemaTM.
• Anastrozole is marketed under the trade name ArimidexTM.
• Letrozole is marketed under the trade names FemaraTM or FemarTM.
• Aminoglutethimide is marketed under the trade name OrimetenTM.
• a combination of the invention comprising a chemotherapeutic agent which is an aromatase inhibitor is particularly useful for the treatment of hormone receptor positive tumors, such as breast tumors.
• antiestrogen as used herein relates to a compound which antagonizes the effect of estrogens at the estrogen receptor level.
• the term includes, but is not limited to tamoxifen, fulvestrant, raloxifene and raloxifene hydrochloride.
• Tamoxifen is marketed under the trade name NolvadexTM.
• Raloxifene hydrochloride is marketed under the trade name EvistaTM.
• Fulvestrant can be administered under the trade name FaslodexTM.
• a combination of the invention comprising a chemotherapeutic agent which is an antiestrogen is particularly useful for the treatment of estrogen receptor positive tumors, such as breast tumors.
• anti-androgen as used herein relates to any substance which is capable of inhibiting the biological effects of androgenic hormones and includes, but is not limited to, bicalutamide (CasodexTM).
• gonadorelin agonist as used herein includes, but is not limited to abarelix, goserelin and goserelin acetate. Goserelin can be administered under the trade name ZoladexTM.
• topoisom erase I inhibitor includes, but is not limited to topotecan, gimatecan, irinotecan, camptothecin and its analogues, 9-nitrocamptothecin and the macromolecular camptothecin conjugate PNU-166148.
• Irinotecan can be administered, e.g. in the form as it is marketed, e.g. under the trademark CamptosarTM.
• Topotecan is marketed under the trade name HycamptinTM.
• topoisomerase II inhibitor includes, but is not limited to the anthracyclines such as doxorubicin (including liposomal formulation, such as CaelyxTM), daunorubicin, epirubicin, idarubicin and nemorubicin, the anthraquinones mitoxantrone and losoxantrone, and the podophillotoxines etoposide and teniposide.
• Etoposide is marketed under the trade name EtopophosTM.
• Teniposide is marketed under the trade name VM 26-Bristol
• Doxorubicin is marketed under the trade name AcriblastinTM or AdriamycinTM.
• Epirubicin is marketed under the trade name FarmorubicinTM.
• Idarubicin is marketed, under the trade name ZavedosTM.
• Mitoxantrone is marketed under the trade name Novantron.
• microtubule active agent relates to microtubule stabilizing, microtubule destabilizing compounds and microtubulin polymerization inhibitors including, but not limited to taxanes, such as paclitaxel and docetaxel; vinca alkaloids, such as vinblastine or vinblastine sulfate, vincristine or vincristine sulfate, and vinorelbine; discodermolides; colchicine and epothilones and derivatives thereof.
• Paclitaxel is marketed under the trade name TaxolTM.
• Docetaxel is marketed under the trade name TaxotereTM.
• Vinblastine sulfate is marketed under the trade name Vinblastin R.PTM.
• Vincristine sulfate is marketed under the trade name FarmistinTM.
• alkylating agent includes, but is not limited to, cyclophosphamide, ifosfamide, melphalan or nitrosourea (BCNU or Gliadel). Cyclophosphamide is marketed under the trade name CyclostinTM. Ifosfamide is marketed under the trade name HoloxanTM.
• histone deacetylase inhibitors or "FIDAC inhibitors” relates to compounds which inhibit the histone deacetylase and which possess antiproliferative activity. This includes, but is not limited to, suberoylanilide hydroxamic acid (SAHA).
• SAHA suberoylanilide hydroxamic acid
• antimetabolite includes, but is not limited to, 5-fluorouracil or 5-FU, capecitabine, gemcitabine, DNA demethylating compounds, such as 5-azacytidine and decitabine, methotrexate and edatrexate, and folic acid antagonists such as pemetrexed.
• Capecitabine is marketed under the trade name XelodaTM.
• Gemcitabine is marketed under the trade name GemzarTM.
• platinum compound as used herein includes, but is not limited to, carboplatin, cis-platin, cisplatinum and oxaliplatin.
• Carboplatin can be administered, e.g., in the form as it is marketed, e.g. under the trademark CarboplatTM.
• Oxaliplatin can be administered, e.g., in the form as it is marketed, e.g. under the trademark EloxatinTM.
• the term "compounds targeting/decreasing a protein or lipid kinase activity; or a protein or lipid phosphatase activity; or further anti-angiogenic compounds” as used herein includes, but is not limited to, protein tyrosine kinase and/or serine and/or threonine kinase inhibitors or lipid kinase inhibitors, such as a) compounds targeting, decreasing or inhibiting the activity of the platelet-derived growth factor-receptors (PDGFR), such as compounds which target, decrease or inhibit the activity of PDGFR, especially compounds which inhibit the PDGF receptor, such as an N-phenyl-2-pyrimidine-amine derivative, such as imatinib, SU101, SU6668 and GFB- 111; b) compounds targeting, decreasing or inhibiting the activity of the fibroblast growth factor- receptors (FGFR); c) compounds targeting, decreasing or inhibiting the activity of the insulin-like growth factor receptor I (IGF
• BCR-Abl kinase and mutants, such as compounds which target decrease or inhibit the activity of c-Abl family members and their gene fusion products, such as an N-phenyl-2-pyrimidine-amine derivative, such as imatinib or nilotinib (AMN107); PD 180970; AG957; NSC 680410; PD173955 from ParkeDavis; or dasatinib (BMS-354825); j) compounds targeting, decreasing or inhibiting the activity of members of the protein kinase C (PKC) and Raf family of serine/threonine kinases, members of the MEK, SRC, JAK/pan-JAK, FAK, PDK1, PKB/Akt, Ras/MAPK, PI3K, SYK, TYK2, BTK and TEC family, and/or members of the cyclin- dependent kinase family (CDK) including staurosporine derivatives, such as midostaurin;
• c-Met receptor compounds which target, decrease or inhibit the activity of c-Met, especially compounds which inhibit the kinase activity of c-Met receptor, or antibodies that target the extracellular domain of c-Met or bind to HGF
• JAK family members J
• PI3K inhibitor includes, but is not limited to compounds having inhibitory activity against one or more enzymes in the phosphatidylinositol-3 -kinase family, including, but not limited to ⁇ , ⁇ , ⁇ , ⁇ , PBK-C2a, PBK-C2p, PBK- C2y, Vps34, pi 10-a, pi 10- ⁇ , pi 10- ⁇ , pi 10- ⁇ , p85-a, ⁇ 85- ⁇ , ⁇ 55- ⁇ , pl50, plOl, and p87.
• PBK inhibitors useful in this invention include but are not limited to ATU-027, SF-1126, DS- 7423, PBI-05204, GSK-2126458, ZSTK-474, buparlisib, pictrelisib, PF-4691502, BYL-719, dactolisib, XL-147, XL-765, and idelalisib.
• Bcl-2 inhibitor includes, but is not limited to compounds having inhibitory activity against B-cell lymphoma 2 protein (Bcl-2), including but not limited to ABT-199, ABT-731, ABT-737, apogossypol, Ascenta's pan-Bcl-2 inhibitors, curcumin (and analogs thereof), dual Bcl-2/Bcl-xL inhibitors (Infinity Pharmaceuticals/Novartis Pharmaceuticals), Genasense (G3139), HA14-1 (and analogs thereof; see WO2008118802), navitoclax (and analogs thereof, see US7390799), H-1 (Shenayng Pharmaceutical University), obatoclax (and analogs thereof, see WO2004106328), S-001 (Gloria Pharmaceuticals), TW series compounds (Univ. of Michigan), and venetoclax.
• the Bcl-2 inhibitor is a small molecule therapeutic.
• the Bcl-2 is a small molecule therapeutic.
• the Bcl-2
• BTK inhibitor includes, but is not limited to compounds having inhibitory activity against Bruton's Tyrosine Kinase (BTK), including, but not limited to AVL-292 and ibrutinib.
• SYK inhibitor includes, but is not limited to compounds having inhibitory activity against spleen tyrosine kinase (SYK), including but not limited to PRT- 062070, R-343, R-333, Excellair, PRT-062607, and fostamatinib.
• BTK inhibitory compounds and conditions treatable by such compounds in combination with compounds of this invention can be found in WO2008039218 and WO2011090760, the entirety of which are incorporated herein by reference.
• PI3K inhibitory compounds and conditions treatable by such compounds in combination with compounds of this invention can be found in WO2004019973, WO2004089925, WO2007016176, US8138347, WO2002088112, WO2007084786, WO2007129161, WO2006122806, WO2005113554, and WO2007044729 the entirety of which are incorporated herein by reference.
• JAK inhibitory compounds and conditions treatable by such compounds in combination with compounds of this invention can be found in WO2009114512, WO2008109943, WO2007053452, WO2000142246, and WO2007070514, the entirety of which are incorporated herein by reference.
• Further anti-angiogenic compounds include compounds having another mechanism for their activity, e.g. unrelated to protein or lipid kinase inhibition e.g. thalidomide (ThalomidTM) and T P-470.
• thalidomide ThalomidTM
• T P-470 thalidomide
• proteasome inhibitors useful for use in combination with compounds of the invention include, but are not limited to bortezomib, disulfiram, epigallocatechin-3-gallate (EGCG), salinosporamide A, carfilzomib, ONX-0912, CEP-18770, and MLN9708.
• Compounds which target, decrease or inhibit the activity of a protein or lipid phosphatase are e.g. inhibitors of phosphatase 1, phosphatase 2A, or CDC25, such as okadaic acid or a derivative thereof.
• Compounds which induce cell differentiation processes include, but are not limited to, retinoic acid, ⁇ - ⁇ - or ⁇ - tocopherol or a- ⁇ - or ⁇ -tocotrienol.
• cyclooxygenase inhibitor as used herein includes, but is not limited to, Cox- 2 inhibitors, 5-alkyl substituted 2-arylaminophenylacetic acid and derivatives, such as celecoxib (CelebrexTM), rofecoxib (VioxxTM), etoricoxib, valdecoxib or a 5-alkyl-2- arylaminophenylacetic acid, such as 5-methyl-2-(2'-chloro-6'-fluoroanilino)phenyl acetic acid, lumiracoxib.
• Cox- 2 inhibitors such as celecoxib (CelebrexTM), rofecoxib (VioxxTM), etoricoxib, valdecoxib or a 5-alkyl-2- arylaminophenylacetic acid, such as 5-methyl-2-(2'-chloro-6'-fluoroanilino)phenyl acetic acid, lumiracoxib.
• bisphosphonates includes, but is not limited to, etridonic, clodronic, tiludronic, pamidronic, alendronic, ibandronic, risedronic and zoledronic acid.
• Etridonic acid is marketed under the trade name DidronelTM.
• Clodronic acid is marketed under the trade name BonefosTM.
• Tiludronic acid is marketed under the trade name SkelidTM.
• Pamidronic acid is marketed under the trade name ArediaTM.
• Alendronic acid is marketed under the trade name FosamaxTM.
• Ibandronic acid is marketed under the trade name BondranatTM.
• Risedronic acid is marketed under the trade name ActonelTM.
• Zoledronic acid is marketed under the trade name ZometaTM.
• mTOR inhibitors relates to compounds which inhibit the mammalian target of rapamycin (mTOR) and which possess antiproliferative activity such as sirolimus (Rapamune®), everolimus (CerticanTM), CCI-779 and ABT578.
• heparanase inhibitor refers to compounds which target, decrease or inhibit heparin sulfate degradation.
• the term includes, but is not limited to, PI-88.
• biological response modifier refers to a lymphokine or interferons.
• inhibitor of Ras oncogenic isoforms such as H-Ras, K-Ras, or N-Ras, as used herein refers to compounds which target, decrease or inhibit the oncogenic activity of Ras; for example, a “farnesyl transferase inhibitor” such as L-744832, DK8G557 or Rl 15777 (ZarnestraTM).
• telomerase inhibitor refers to compounds which target, decrease or inhibit the activity of telomerase. Compounds which target, decrease or inhibit the activity of telomerase are especially compounds which inhibit the telomerase receptor, such as telomestatin.
• methionine aminopeptidase inhibitor refers to compounds which target, decrease or inhibit the activity of methionine aminopeptidase.
• Compounds which target, decrease or inhibit the activity of methionine aminopeptidase include, but are not limited to, bengamide or a derivative thereof.
• proteasome inhibitor refers to compounds which target, decrease or inhibit the activity of the proteasome.
• Compounds which target, decrease or inhibit the activity of the proteasome include, but are not limited to, Bortezomib (VelcadeTM) and MLN 341.
• matrix metalloproteinase inhibitor or (“MMP” inhibitor) as used herein includes, but is not limited to, collagen peptidomimetic and nonpeptidomimetic inhibitors, tetracycline derivatives, e.g. hydroxamate peptidomimetic inhibitor batimastat and its orally bioavailable analogue marimastat (BB-2516), prinomastat (AG3340), metastat (NSC 683551) BMS-279251 , BAY 12-9566, TAA211 , MMI270B or AAJ996.
• MMP matrix metalloproteinase inhibitor
• FMS-like tyrosine kinase inhibitors which are compounds targeting, decreasing or inhibiting the activity of FMS-like tyrosine kinase receptors (Flt-3R); interferon, ⁇ - ⁇ -D-arabinofuransylcytosine (ara-c) and bisulfan; and ALK inhibitors, which are compounds which target, decrease or inhibit anaplastic lymphoma kinase.
• FMS-like tyrosine kinase receptors are especially compounds, proteins or antibodies which inhibit members of the Flt-3R receptor kinase family, such as PKC412, midostaurin, a staurosporine derivative, SU11248 and MLN518.
• HSP90 inhibitors includes, but is not limited to, compounds targeting, decreasing or inhibiting the intrinsic ATPase activity of HSP90; degrading, targeting, decreasing or inhibiting the HSP90 client proteins via the ubiquitin proteosome pathway.
• Compounds targeting, decreasing or inhibiting the intrinsic ATPase activity of HSP90 are especially compounds, proteins or antibodies which inhibit the ATPase activity of HSP90, such as 17-allylamino,17-demethoxygeldanamycin (17AAG), a geldanamycin derivative; other geldanamycin related compounds; radicicol and FID AC inhibitors.
• antiproliferative antibodies includes, but is not limited to, trastuzumab (HerceptinTM), Trastuzumab-DMl, erbitux, bevacizumab (AvastinTM), rituximab (Rituxan ® ), PR064553 (anti-CD40) and 2C4 Antibody.
• antibodies is meant intact monoclonal antibodies, polyclonal antibodies, multispecific antibodies formed from at least 2 intact antibodies, and antibodies fragments so long as they exhibit the desired biological activity.
• compounds of the current invention can be used in combination with standard leukemia therapies, especially in combination with therapies used for the treatment of AML.
• compounds of the current invention can be administered in combination with, for example, farnesyl transferase inhibitors and/or other drugs useful for the treatment of AML, such as Daunorubicin, Adriamycin, Ara-C, VP- 16, Teniposide, Mitoxantrone, Idarubicin, Carboplatinum and PKC412.
• drugs useful for the treatment of AML such as Daunorubicin, Adriamycin, Ara-C, VP- 16, Teniposide, Mitoxantrone, Idarubicin, Carboplatinum and PKC412.
• anti-leukemic compounds include, for example, Ara-C, a pyrimidine analog, which is the 2 -alpha-hydroxy ribose (arabinoside) derivative of deoxycytidine. Also included is the purine analog of hypoxanthine, 6-mercaptopurine (6-MP) and fludarabine phosphate.
• HDAC hi stone deacetylase
• SAHA suberoylanilide hydroxamic acid
• HDAC inhibitors include MS275, SAHA, FK228 (formerly FR901228), Trichostatin A and compounds disclosed in US 6,552,065 including, but not limited to, N-hydroxy-3-[4-[[[2-(2-methyl-lH-indol-3-yl)-ethyl]- amino]methyl]phenyl]- 2E-2-propenamide, or a pharmaceutically acceptable salt thereof and N-hydroxy-3-[4-[(2- hydroxyethyl) ⁇ 2-(lH-indol-3-yl)ethyl]-amino]methyl]phenyl]-2E-2- propenamide, or a pharmaceutically acceptable salt thereof, especially the lactate salt.
• Somatostatin receptor antagonists as used herein refer to compounds which target, treat or inhibit the somatostatin receptor such as octreotide, and SOM230.
• Tumor cell damaging approaches refer to approaches such as ionizing radiation.
• the term "ionizing radiation” referred to above and hereinafter means ionizing radiation that occurs as either electromagnetic rays (such as X-rays and gamma rays) or particles (such as alpha and beta particles). Ionizing radiation is provided in, but not limited to, radiation therapy and is known in the art. See Hellman, Principles of Radiation Therapy, Cancer, in Principles and Practice of Oncology, Devita et al., Eds., 4 th Edition, Vol. 1 , pp. 248-275 (1993).
• EDG binders and ribonucleotide reductase inhibitors.
• EDG binders refers to a class of immunosuppressants that modulates lymphocyte recirculation, such as FTY720.
• ribonucleotide reductase inhibitors refers to pyrimidine or purine nucleoside analogs including, but not limited to, fludarabine and/or cytosine arabinoside (ara-C), 6-thioguanine, 5-fluorouracil, cladribine, 6-mercaptopurine (especially in combination with ara-C against ALL) and/or pentostatin.
• Ribonucleotide reductase inhibitors are especially hydroxyurea or 2-hydroxy-lH-isoindole-l ,3-dione derivatives.
• VEGF such as l-(4-chloroanilino)-4-(4-pyridylmethyl)phthalazine or a pharmaceutically acceptable salt thereof, l-(4-chloroanilino)-4-(4-pyridylmethyl)phthalazine succinate; AngiostatinTM; EndostatinTM; anthranilic acid amides; ZD4190; ZD6474; SU5416; SU6668; bevacizumab; or anti-VEGF antibodies or anti-VEGF receptor antibodies, such as rhuMAb and RHUFab, VEGF aptamer such as Macugon; FLT-4 inhibitors, FLT-3 inhibitors, VEGFR-2 IgGI antibody, Angiozy
• Photodynamic therapy refers to therapy which uses certain chemicals known as photosensitizing compounds to treat or prevent cancers.
• Examples of photodynamic therapy include treatment with compounds, such as VisudyneTM and porfimer sodium.
• Angiostatic steroids refers to compounds which block or inhibit angiogenesis, such as, e.g., anecortave, triamcinolone, hydrocortisone, 11-a-epihydrocotisol, cortex ol one, 17a-hydroxyprogesterone, corticosterone, desoxycorticosterone, testosterone, estrone and dexamethasone.
• angiogenesis such as, e.g., anecortave, triamcinolone, hydrocortisone, 11-a-epihydrocotisol, cortex ol one, 17a-hydroxyprogesterone, corticosterone, desoxycorticosterone, testosterone, estrone and dexamethasone.
• Implants containing corticosteroids refers to compounds, such as fluocinolone and dexamethasone.
• chemotherapeutic compounds include, but are not limited to, plant alkaloids, hormonal compounds and antagonists; biological response modifiers, preferably lymphokines or interferons; antisense oligonucleotides or oligonucleotide derivatives; shRNA or siRNA; or miscellaneous compounds or compounds with other or unknown mechanism of action.
• a compound of the current invention may also be used in combination with known therapeutic processes, for example, the administration of hormones or radiation.
• a provided compound is used as a radiosensitizer, especially for the treatment of tumors which exhibit poor sensitivity to radiotherapy.
• a compound of the current invention can be administered alone or in combination with one or more other therapeutic compounds, possible combination therapy taking the form of fixed combinations or the administration of a compound of the invention and one or more other therapeutic compounds being staggered or given independently of one another, or the combined administration of fixed combinations and one or more other therapeutic compounds.
• a compound of the current invention can besides or in addition be administered especially for tumor therapy in combination with chemotherapy, radiotherapy, immunotherapy, phototherapy, surgical intervention, or a combination of these. Long-term therapy is equally possible as is adjuvant therapy in the context of other treatment strategies, as described above. Other possible treatments are therapy to maintain the patient's status after tumor regression, or even chemopreventive therapy, for example in patients at risk.
• Those additional agents may be administered separately from an inventive compound- containing composition, as part of a multiple dosage regimen.
• those agents may be part of a single dosage form, mixed together with a compound of this invention in a single composition. If administered as part of a multiple dosage regime, the two active agents may be submitted simultaneously, sequentially or within a period of time from one another normally within five hours from one another.
• the term “combination,” “combined,” and related terms refers to the simultaneous or sequential administration of therapeutic agents in accordance with this invention.
• a compound of the present invention may be administered with another therapeutic agent simultaneously or sequentially in separate unit dosage forms or together in a single unit dosage form.
• the present invention provides a single unit dosage form comprising a compound of the current invention, an additional therapeutic agent, and a pharmaceutically acceptable carrier, adjuvant, or vehicle.
• compositions of this invention should be formulated so that a dosage of between 0.01 - 100 mg/kg body weight/day of an inventive compound can be administered.
• compositions which comprise an additional therapeutic agent that additional therapeutic agent and the compound of this invention may act synergistically. Therefore, the amount of additional therapeutic agent in such compositions will be less than that required in a monotherapy utilizing only that therapeutic agent. In such compositions a dosage of between 0.01 - 1,000 ⁇ g/kg body weight/day of the additional therapeutic agent can be administered.
• the amount of additional therapeutic agent present in the compositions of this invention will be no more than the amount that would normally be administered in a composition comprising that therapeutic agent as the only active agent.
• the amount of additional therapeutic agent in the presently disclosed compositions will range from about 50% to 100% of the amount normally present in a composition comprising that agent as the only therapeutically active agent.
• the compounds of this invention, or pharmaceutical compositions thereof, may also be incorporated into compositions for coating an implantable medical device, such as prostheses, artificial valves, vascular grafts, stents and catheters.
• an implantable medical device such as prostheses, artificial valves, vascular grafts, stents and catheters.
• Vascular stents for example, have been used to overcome restenosis (re-narrowing of the vessel wall after injury).
• patients using stents or other implantable devices risk clot formation or platelet activation. These unwanted effects may be prevented or mitigated by pre-coating the device with a pharmaceutically acceptable composition comprising a kinase inhibitor.
• Implantable devices coated with a compound of this invention are another embodiment of the present invention.
• the compounds of this invention may be prepared or isolated in general by synthetic and/or semi-synthetic methods known to those skilled in the art for analogous compounds and by methods described in detail in the Examples, herein.
• LG includes, but is not limited to, halogens (e.g. fluoride, chloride, bromide, iodide), sulfonates (e.g. mesylate, tosylate, benzenesulfonate, brosylate, nosylate, triflate), diazonium, and the like.
• halogens e.g. fluoride, chloride, bromide, iodide
• sulfonates e.g. mesylate, tosylate, benzenesulfonate, brosylate, nosylate, triflate
• diazonium and the like.
• oxygen protecting group includes, for example, carbonyl protecting groups, hydroxyl protecting groups, etc. Hydroxyl protecting groups are well known in the art and include those described in detail in Protecting Groups in Organic Synthesis, T. W. Greene and P. G. M. Wuts, 3 rd edition, John Wiley & Sons, 1999, and Philip Kocienski, in "Protecting Groups", Georg Thieme Verlag Stuttgart, New York, 1994, the entireties of which is incorporated herein by reference.
• Suitable hydroxyl protecting groups include, but are not limited to, esters, allyl ethers, ethers, silyl ethers, alkyl ethers, arylalkyl ethers, and alkoxyalkyl ethers.
• esters include formates, acetates, carbonates, and sulfonates.
• Specific examples include formate, benzoyl formate, chloroacetate, trifluoroacetate, methoxyacetate, triphenylmethoxyacetate, p-chlorophenoxyacetate, 3-phenylpropionate, 4- oxopentanoate, 4,4-(ethylenedithio)pentanoate, pivaloate (trimethylacetyl), crotonate, 4-methoxy- crotonate, benzoate, p-benylbenzoate, 2,4,6-trimethylbenzoate, carbonates such as methyl, 9- fluorenylmethyl, ethyl, 2,2,2-trichloroethyl, 2-(trimethylsilyl)ethyl, 2-(phenylsulfonyl)ethyl, vinyl, allyl, and p-nitrobenzyl.
• silyl ethers examples include trimethylsilyl, triethylsilyl, t-butyldimethylsilyl, t-butyldiphenylsilyl, triisopropylsilyl, and other trialkylsilyl ethers.
• Alkyl ethers include methyl, benzyl, p-methoxybenzyl, 3,4-dimethoxybenzyl, trityl, t-butyl, allyl, and allyloxycarbonyl ethers or derivatives.
• Alkoxyalkyl ethers include acetals such as methoxymethyl, methylthiomethyl, (2-methoxyethoxy)methyl, benzyloxymethyl, beta-
• arylalkyl ethers include benzyl, p-methoxybenzyl (MPM), 3,4-dimethoxybenzyl, O-nitrobenzyl, p-nitrobenzyl, p-halobenzyl, 2,6-dichlorobenzyl, p-cyanobenzyl, and 2- and 4-picolyl.
• Amino protecting groups are well known in the art and include those described in detail in Protecting Groups in Organic Synthesis, T. W. Greene and P. G. M. Wuts, 3 rd edition, John Wiley & Sons, 1999, and Philip Kocienski, in "Protecting Groups", Georg Thieme Verlag Stuttgart, New York, 1994, the entireties of which is incorporated herein by reference.
• Suitable amino protecting groups include, but are not limited to, aralkylamines, carbamates, cyclic imides, allyl amines, amides, and the like.
• Examples of such groups include t-butyloxycarbonyl (BOC), ethyloxycarbonyl, methyloxycarbonyl, trichloroethyloxycarbonyl, allyloxycarbonyl (Alloc), benzyloxocarbonyl (CBZ), allyl, phthalimide, benzyl (Bn), fluorenylmethylcarbonyl (Fmoc), formyl, acetyl, chloroacetyl, dichloroacetyl, trichloroacetyl, phenylacetyl, trifluoroacetyl, benzoyl, and the like.
• R' is a group such as Ci- 6 aliphatic, 5- to 8-membered aromatic ring, or other functionality compatible with an ester; and R 1 , R 2 , R 3 , and R 6 are selected consistent with formula I above and below and in classes and subclasses as described herein.
• the present invention provides methods for preparing compounds of formula GS4 as described in Scheme 1.
• An optionally substituted benzaldehyde may be condensed with an azidoacetate in the presence of base such as sodium hydroxide or sodium methoxide to give intermediate GS1.
• Heating GS1 in a solvent such as toluene e.g., at reflux provides the indole-2-carboxylate ester.
• the indole nitrogen is alkylated using an appropriate alkyl halide such as methyl or ethyl iodide and a suitable base such as, but not limited, to sodium hydride, potassium tert-butoxide, or potassium carbonate in a suitable solvent to provide GS3.
• a suitable base such as, but not limited, to sodium hydride, potassium tert-butoxide, or potassium carbonate in a suitable solvent to provide GS3.
• the ester of GS3 is hydrolyzed using a base such as LiOH, KOH or NaOH in a solvent such as a mixture of water and THF to provide an intermediate used in the synthesis of compounds of the invention of general structure GS4.
• GS3 may be treated with appropriate reagents such as POCb and DMF to give GS3'.
• the aldehyde in GS3' is then reduced to a methyl group with appropriate reagents such as Et 3 SiH and TFA to give GS4' .
• hydrolysis of the ester in GS4' provides an intermediate used in the synthesis of compounds of the invention of general structure GS5'.
• Optionally substituted benzoic acids e.g. bromide or other halide-substituted
• ester GS5 can be converted to the ester GS5 by reacting with reagents such as thionyl chloride in methanol. Heating GS5 in a mixture of acetonitnle and tetrahydrofuran with sodium hydride leads to GS6. Reduction with nickel chloride hexahydrate and sodium borohydride in tetrahydrofuran gives GS7.
• the amine can be protected under standard conditions with di-tert-butyl dicarbonate and iodine in methanol.
• the alcohol of GS8 can then be transformed to the indoline-dione by coupling with isoindoline-l,3-dione (phthalimide), diisopropyl azodicarboxylate (DIAD), and triphenylphosphine in tetrahydrofuran.
• phthalimide isoindoline-l,3-dione
• DIAD diisopropyl azodicarboxylate
• triphenylphosphine in tetrahydrofuran.
• Heating GS9 with hydrazine hydrate in an appropriate solvent such as methanol or ethanol leads to amine GS10.
• the bromide or other halide can then be removed with conditions such as hydrogenation with palladium/charcoal under hydrogen atmosphere (50 psi) in methanol to give GS11.
• GS9 can be coupled with Pd(dppf)Cl 2 » CH 2 Cl 2 and triethylamine in ethanol in the presence of carbon monoxide (50 psi) to convert the bromide to ester GS 12.
• the amine GS 13 can then be formed as described for GS 10.
• Rl is a group such as those disclosed in Table 1R 2 can be one of various groups including CF 3 , alkyl, cycloalkyl, or nitrogen containing Ci-6 alkyl and cycloalkyl
• a catalyst such as Pd(dppf)Cl 2 in an appropriate solvent such as ethanol or methanol in the presence of carbon monoxide (50 psi) with heating to form ester GS 14.
• the carbonyl can then converted to oxime GS 15 by treatment with appropriate reagents such as hydroxylamine hydrochloride and sodium acetate in ethanol with heating.
• GS 15 can then be reduced under standard hydrogenation conditions such as Raney nickel under hydrogen atmosphere (50 psi) in ethanol to form GS 16.
• the bromophenylketone can be coupled with (3-ethoxy-3-oxo- propanoyl) oxypotassium by reacting with allyl(chloro)palladium, Xantphos, and DMAP in xylene to form GS17.
• the carbonyl can then be further elaborated to oxime GS18 and amine GS19 via the method described for GS15 and GS16, respectively.
• an alkyl halide can be added using sodium hydride as a base in N,N-dimethylformamide to form GS22.
• the bromide can be converted to the ester as previously described for GS12 and GS14.
• Hydrolysis of the nitrile to the amide GS24 may be achieved with exposure to conditions such as hydrogen peroxide and potassium carbonate in dimethyl sulfoxide.
• Formation of amine GS25 can be accomplished by reacting the amide with PhI(CF 3 C02)2 in acetonitrile/water.
• KHMDS potassium bis(trimethylsilyl)amide
• Amide GS27 and amine GS28 can be formed as previously described for GS24 and GS25, respectively.
• an ester may be installed at the bromine position of GS29 by treatment with Pd(dppf)Cl 2 » CH 2 Cl 2 and triethylamine in ethanol in the presence of CO gas (50 psi) to give GS32.
• Deprotection to form GS33 can be achieved as described for GS31.
• R 1 alkyl
• R 2 H, alkyl
• Intermediate GS31 in Scheme 6 can be treated with iodine and di-tert-butyl dicarbonate in dichloromethane to form protected amine GS34.
• Reacting GS34 with appropriate reagents such as 2,2-dimethoxypropane and ⁇ -toluenesulfonic acid (TsOH) in acetone gives GS35.
• GS35 can then be alkylated with a variety of alky halides with an appropriate base such as LiHMDS in an appropriate solvent such as N,N-dimethylformamide or tetrahydrofuran to give either the mono or bis alkylated product GS36.
• Deprotection with hydrogen chloride in ethyl acetate or dioxane gives amine GS37.
• R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 6 , R 7 , and R 8 are selected consistent with formula I, or subformulae thereof, as described above and below.
• Conversion of the acid group in GS4 in Scheme 7 to the acid chloride can be done using procedures well known to one skilled in the art.
• the acid GS4 can be treated with oxalyl chloride or thionyl chloride with a catalytic amount of DMF in a solvent such as DCM.
• Reaction of the acid chloride intermediate with an amine of choice in the presence of a base such as triethylamine or diisopropylethylamine affords GS38.
• Hydrolysis of the ester can be achieved with lithium hydroxide in tetrahydrofuran and water to form the final acid product GS39.
• R 1 , R 2 , R 3 , R 4 , and R 6 are selected consistent with formula I, or subformulae thereof, as described above and below.
• Acid GS4 in Scheme 8 can be coupled with a variety of amines with EDCI and HOBt in DMF to form GS40.
• Hydrolysis of the ester to form GS41 can be effected as described for GS39 above.
• Acid GS4 in Scheme 9 can be coupled with a variety of amines to form product GS42 as previously described for GS38.
• Deprotection of the Boc with trifluoracetic acid in dichloromethane can be used to give amine GS43.
• the amine can then be coupled with N- acetylsulfamoyl chloride in dichloromethane to form final product GS44.
• AIBN 2,2'-azo bisisobutyronitrile Anhyd: anhydrous Aq: aqueous
• BINAP 2,2'-bi s(diphenylphosphino)- 1 , 1 '-binaphthyl
• B3 ⁇ 4 Borane
• Bn benzyl
• DIBAL-H diisobutylaluminum hydride
• DIPEA or DIEA N,N-diisopropylethylamine
• HATU ⁇ , ⁇ , ⁇ ' , ⁇ ' -tetramethyl-0-(7-azabenzotriazol- 1 -yl)uronium hexafluorophosphate HC1: hydrochloric acid
• KHMDS potassium hexamethyldisilazide
• LiHMDS lithium bis(trimethylsilyl)amide
• Me 2 S dimethyl sulfide
• MeONa sodium methoxide
• NBS N-bromosuccinimide
• PBS phosphate buffered saline
• PE petroleum ether
• TfAA trifluoromethanesulfonic anhydride
• TIPS triisopropylsilyl
• TMEDA tetramethylethylenediamine
• pTSA para-toluenesulfonic acid
• Proton NMR (3 ⁇ 4 NMR) is conducted in deuterated solvent.
• one or more 3 ⁇ 4 shifts overlap with residual protio solvent signals; these signals have not been reported in the experimental provided hereinafter.
• LCMS was recorded on an Agilent 1200 Series LC/MSD or Shimadzu LCMS2020 equipped with electro-spray ionization and quadruple MS detector [ES+ve to give MH + ] and equipped with Chromolith Flash RP-18e 25*2.0 mm, eluting with 0.0375 vol% TFA in water (solvent A) and 0.01875 vol% TFA in acetonitrile (solvent B).
• LCMS was recorded on an Agilent 1200 Series LC/MSD or Shimadzu LCMS 2020 equipped with electro-spray ionization and quadruple MS detector [ES+ve to give MH + ] and equipped with Xbridge C18, 2.1X50 mm columns packed with 5 mm C18-coated silica or Kinetex EVO C18 2.1X30mm columns packed with 5 mm C18-coated silica, eluting with 0.05 vol% ⁇ 3 ⁇ 2 ⁇ in water (solvent A) and acetonitrile (solvent B).
• Step 4 4, 5-Dichloro-l-methyl-indole-2-carboxylic acid
• Step 2 Methyl(Z)-2-azido-3-(2.3-dichloro-4-methoxy-phenvnprop-2-enoate
• Step 2 Methyl 6-r2-r2-rtert-butyl(dimethvnsilyl1oxyethylamino1-2-oxo-ethoxy1-4.5- di chl pro- 1 -methyl -indol e-2-carb oxyl ate
• Step 3 Methyl 6-r2-r2-rtert-butyl(dimethvnsilyl1oxyethylamino1-2-oxo-ethoxy1-4.5- di chl pro- 1 -methyl -indol e-2-carb oxyl ate
• TP a splutipn pf methyl 6-[2-[2-[tert-butyl(dimethyl)silyl]pxyethylaminp]-2-pxp- ethpxy]- 4,5-dichlprp-l-methyl-indple-2-carbpxylate (750 mg, 1.53 mmpl) in a mixture splvent pf H 2 0 (1.00 mL) and THF (7.00 mL) was added and Li OH (147 mg, 6.13 mmpl). The reactipn mixture was stirred at rt fpr 12 hpurs.
• Step 1 ( ⁇ )-Ethyl 5-chloro-6-r(2-oxooxazolidin-5-yl)methoxy1-lH-indole-2- carboxylate
• Step 2 ( ⁇ )-5-Chloro-6-r(2-oxooxazolidin-5-yl)methoxy1-lH-indole-2-carboxylic acid
• Step 1 2-Chloro-4-methoxy-3-methyl-benzaldehvde
• Step 2 Methyl (Z)-2-azido-3-(2-chloro-4-methoxy-3-methyl-phenvnprop-2-enoate
• Step 3 Methyl 4-chloro-6-methoxy-5-methyl-lH-indole-2-carboxylate
• Methyl (Z)-2-azido-3-(2-chloro-4-methoxy-3-methyl-phenyl) prop-2-enoate (4.00 g, 14.2 mmol) was added to xylene (100 mL) in one portion at rt, then the solution was stirred at 120 °C for 12 hrs. The mixture was concentrated in vacuo. The residue was washed with petroleum ethenethyl acetate (10: 1, 50 mL) to give the title compound.
• Step 4 Methyl 4-chloro-6-methoxy-L5-dimethyl-indole-2-carboxylate
• Step 5 4-chloro-6-methoxy-L5-dimethyl-indole-2-carboxylic acid
• Step 1 2-Chloro-3-fluoro-4-methoxybenzaldehyde
• Step 2 (Z)-methyl 2-azido-3-(2-chloro-3-fluoro-4-methoxyphenyl)acrylate
• Step 3 Methyl 4-chloro-5-fluoro-6-methoxy-lH-indole-2-carboxylate
• Step 4 Methyl 4-chloro-5-fluoro-6-methoxy-l-methyl-lH-indole-2-carboxylate
• Step 1 Methyl-2-azido-3-(2-chloro-3-methoxy-phenyl)prop-2-enoate
• Step 2 Methyl -4-chloro-5-methoxy-lH- indole-2-carboxylate
• Methyl-2-azido-3-(2-chloro-3-methoxy-phenyl)prop-2-enoate (2.00 g, 7.47 mmol) was dissolved in xylol (200 mL) and the mixture was stirred at 180 °C for 30 min. On completion, the reaction mixture was concentrated in vacuo to afford a residue. The residue was triturated with petroleum ether: ethyl acetate (10: 1) to give the title compound.
• 3 ⁇ 4 NMR (400MHz, DMSO- ⁇ f 6 ) ⁇ 12.16 (br.
• Step 4 4-Chloro-5-methoxy-l- methyl-indole-2-carboxylic acid
• Step 3 ( ⁇ )-3-Amino-l-(3-bromophenvnpropan-l-ol
• Step 4 ( ⁇ )-Tert-butyl N-[3-(3-bromophenyl)-3-hydroxy-propyl]carbamate
• Step 6 ( ⁇ )-Tert-butyl N-r3-amino-3-(3-bromophenvDpropyHcarbamate [00335] To a mixture of ( ⁇ )-tert-butyl N-[3-(3-bromophenyl)-3-(l,3-dioxoisoindolin-2- yl)propyl] carbamate (2.00 g, 4.35 mmol) in methanol (10.0 mL) was added hydrazine hydrate (872 mg, 17.4 mmol) in one portion at rt under nitrogen atmosphere. The mixture was stirred at 62 °C and stirred for 10 hours. On completion, the reaction was concentrated in vacuo.
• Step 7 ( ⁇ )-Tert-butyl N-(3-amino-3-phenyl-propyl)carbamate
• Step 1 ( ⁇ )-Ethyl 3-[3-(tert-butoxycarbonylamino)-l-(L3-dioxoisoindolin-2- yPpropyllbenzoate
• Step 2 ( ⁇ )-Ethyl 3-[l-amino-3-(tert-butoxycarbonylamino)propyl "
• Step 1 -3-(4-Bromophenyl)-3-oxo-propanenitrile
• sodium hydride (1.09 g, 27.2 mmol) portion-wise at rt under nitrogen.
• methyl 4- bromobenzoate (4.50 g, 20.9 mmol) was added to the mixture and then the resulting mixture was heated to 67 °C and stirred for 2 hours.
• the reaction was cooled to rt and 1 N hydrochloric acid solution (50 mL) was added.
• Step 2 -3-Amino-l-(4-bromophenyl)propan-l-ol
• Step 3 Tert-butyl N-[3-(4-bromophenyl)-3-hydroxy-propyl]carbamate .
• Step 4 Tert-butyl N-r3-(4-bromophenvn-3-(l. 3-dioxoisoindolin-2- yPpropyl] carbamate
• Step 5 Ethyl 4-[3-(tert-butoxycarbonylamino)-l-(L 3-dioxoisoindolin-2- yPpropyllbenzoate
• Step 6 Ethyl 4-r i-amino-3-(tert-butoxycarbonylamino)propyl1benzoate
• Step 1 2-Amino-l-(4-bromophenyl)ethanone
• Step 3 Ethyl 4-(2-((tert-butoxycarbonyl)amino)acetyl)benzoate
• Step 5 ( ⁇ )-Ethyl 4-(l-amino-2-((tert-butoxycarbonyl)amino)ethyl)benzoate
• Step 2 Tert-butyl N-r3-(4-bromophenyl)-3-oxo-propyl1-N-methyl-carbamate
• Step 3 Ethyl 4-r3-rtert-butoxycarbonyl(methyl)amino1propanoyl1benzoate
• Step 4 Ethyl 4-IYE and Z)-C-r2-rtert-butoxycarbonyl(methvnamino1ethyl1-N- hydroxy-carbonimidoyll- benzoate
• Step 5 ( ⁇ )-Ethyl 4-[l-amino-3-[tert-butoxycarbonyl(methyl)aminolpropyllbenzoate
• Step 2 Methyl 4-(2.2.2 rifluoro-l-(hvdroxyimino)ethv0benzoate
• methyl 4-(2,2,2-trifluoroacetyl)benzoate 336 mg, 1.45 mmol
• potassium acetate 426 mg, 4.35 mmol
• hydroxyl amine hydrochloride 302 mg, 4.35 mmol
• Step 3 ( ⁇ )-Methyl 4-(l-amino-2.2.2-trifluoroethvDbenzoate
• Step 1 (S)-methyl 2-amino-2-(4-hvdroxyphenyl)acetate
• Step 2 (S)-methyl 2-((tert-butoxycarbonyl)amino)-2-(4-hvdroxyphenyl)acetate
• Step 3 (S)-tert-butyl (2-hvdroxy-l-(4-hvdroxyphenyl)ethyl)carbamate
• Step 4 (S)-tert-butyl 4-(4-hvdroxyphenyl)-2,2-dimethyloxazolidine-3-carboxylate
• Step 5 (SVteit-butyl 2.2-dimethyl-4-(4-(((trifluoromethvnsulfonvnoxy) phenvDoxazolidine-3-carboxylate
• Step 6 (SVteit-butyl 4-(4-(methoxycarbonvnphenvn-2.2-dimethyloxazolidine-3- carboxylate
• Step 7 (S)-methyl 4-(l-amino-2-hvdroxyethvnbenzoate
• Step 1 Methyl 4-(l-(hydroxyimino)ethyl)benzoate
• Step 2 ( ⁇ )-Methyl 4-(l-aminoethyl)benzoate
• Step 1 ( ⁇ )-(4-Bromophenyl)-cyclopropyl-methanamine
• Step 1 ( ⁇ )-2-(4-Bromophenyl)-3-cvclopropyl-propanenitrile
• Step 2 ( ⁇ )-Ethyl 4-(l-cvano-2-cvclopropyl-ethvnbenzoate
• Step 3 ( ⁇ )-Ethyl 4 2-amino-l-(cvclopropylmethyl)-2-oxo-ethyl "
• benzoate [00415] To a solution of ( ⁇ )-ethyl 4-(l-cyano-2-cyclopropyl-ethyl)benzoate (200 mg, 822 umol) and potassium carbonate (45.5 mg, 328 umol) in dimethyl sulfoxide (6 mL) was added hydrogen peroxide (745 mg, 6.57 mmol 30% solution). The reaction mixture was stirred at rt for 3 hrs.
• Step 1 Ethyl 4-(l-cvano-l-methyl-ethv0benzoate
• ethyl 4-(cyanomethyl)benzoate (2.50 g, 13.2 mmol) in anhydrous tetrahydrofuran (80 mL) was added potassium bis(trimethylsilyl)amide (1 M, 52.8 mL) at -10 °C.
• methyl iodide (9.38 g, 66.0 mmol) was added.
• the reaction mixture was warmed to rt and stirred for 3.5 hrs.
• Step 2 Ethyl 4-(2-amino- 1.1 -dimethyl -2-oxo-ethvObenzoate
• Step 2 Tert-butyl 2-(4-(l-(hvdroxyimino)ethvnphenvnacetate
• Step 3 ( ⁇ )-Teit-butyl 2-(4-(l-aminoethyl)phenvnacetate
• Step 2 Ethyl 3-fluoro-4-(l-(hvdroxyimino)ethvnbenzoate
• Step 1 Ethyl 2-(4-acetyl-3-fluoro-phenylacetate
• Step 2 Ethyl 2-(3-fluoro-4-(l-(hvdroxyimino)ethvnphenvnacetate
• Step 3 ( ⁇ )-Ethyl 244-0 -aminoethvn-3-fluoro-phenvHacetate
• Step 1 ( ⁇ )-Methyl 3-chloro-4-(l-cvanoethvnbenzoate
• methyl 3-chloro-4-(cyanomethyl)benzoate (1.00 g, 4.77 mmol) in tetrahydrofuran (10 mL) was added potassium bis(trimethylsilyl)amide (1 M, 5.7 mL) at 0 °C.
• the mixture was stirred at 0 °C for 0.5 h.
• methyl iodide (677 mg, 4.77 mmol) was added and the reaction mixture was stirred at rt for 0.5 h. On completion, the mixture was concentrated in vacuo to give the title compound.
• Step 2 ( ⁇ )-Methyl 4-(l-amino-l-oxopropan-2-yl)-3-chlorobenzoate
• Step 1 N-r(l S)-l-(4-bromophenvn-2-rtert-butyl(dimethvnsilylloxy-ethyll-2-methyl - propane-2-sulfinamide
• Step 2 Ethyl-2-r4-r(l S)-2-rtert-butyl(dimethvnsilylloxy-l-(tert-butylsulfinylamino) ethyl 1 -phenyl ] acetate
• Step 3 (S)-Ethyl 2-(4-(l-amino-2-hvdroxyethvnphenvnacetate
• Step. 1 (S)-N-(YSV 1 -(4-Bromo-2-fluorophenyl V2-((tert- butyldimethylsilyl)oxy)ethyl)-2-methylpropane-2-sulfinamide
• Step 2- Ethyl 2-r4-r(l S)-2-rtert-butyl(dimethvnsilylloxy-l-rr(S)- tert- butyl sulfinyl 1 aminol ethyl 1 -3 -fluoro-phen yl 1 acetate
• Step 3 (S)-Ethyl 2-(4-(l-amino-2-hvdroxyethvn-3-fluorophenvnacetate
• Step 1 Ethyl 4-r(l S)-2-rtert-butyl(dimethvnsilvHoxy-l-rr(S)-tert- butylsulfinyllamino "
• Step 2 Ethyl 4 (l S)-l-amino-2 ivdroxy-ethyl1-3-fluoro-benzoate
• Step 1 Tert-butyl-r(Z)-4-rtert-butyl(dimethvnsilyl1oxybut-2-enoxy1-dimethyl-silane
• Step 2 2 Tert-butyl(dimethvnsilyl1oxyacetaldehvde
• Step 3 (S.E)-N-(2-((tert-butyldimethylsilvnoxy)ethylidene)-2-methylpropane-2- sulfinamide
• Step 4 (S)-N-((S)-l-(4-bromo-2-chlorophenvn-2-((tert-butyldimethylsilvnoxy)ethvn -2-methylpropane-2-sulfinamide
• n-butyllithium (2.42 g, 37.8 mmol) at -78 °C under nitrogen atmosphere
• (S,E)-N-(2-((tert-butyldimethylsilyl)oxy)ethylidene)-2-methylpropane-2- sulfinamide (10.0 g, 36.0 mmol) in tetrahydrofuran (100 mL) was added into the mixture dropwise, the reaction was stirred at this temperature
• Step 5 Ethyl 4-r(l S)-2-rtert-butyl(dimethvnsilvHoxy-l-rr(S)-tert- butylsulfinyl "
• Step 6 Ethyl 4 (l S)-l-amino-2-hvdroxy-ethyl1-3-chloro-benzoate
• Step 1 Ethyl 4-r(l S)-2-rtert-butyl(dimethvnsilylloxy-l-rr(S)-tert- butylsulfinyllaminolethyll- 3-chloro-benzoate
• Step 2 2-r4-r(l S)-l-amino-2-hvdroxy-ethyl1-3-chloro-phenyl1acetate
• Step 1 N-r(l S)-l-(4-bromo-2-methyl-phenvn-2-rtert-butyl(dimethvnsilylloxy- ethyll-2-methyl- propane-2-sulfinamide
• Step 2 Ethyl 4-r(l S)-2-rtert-butyl(dimethvnsilvHoxy-l-rr(S)-tert- butylsulfinyllaminolethyll- 3-methyl-benzoate
• Step 3 Ethyl 4-r(l S)-l-amino-2-hvdroxy-ethyl1-3-methyl-benzoate
• Step 1 Ethyl 2-r4-r(l S)-2-rtert-butyl(dimethyl)silvnoxy-l-rr(S)- tert- butyl sulfinyl] amino] ethyl] -3 -methyl -phenyl] acetate
• Step 2 Ethyl 2-r4-r(l S)-l-amino-2-hvdroxy-ethyl1-3-methyl-phenyl1acetate
• Step 1 - ⁇ - ⁇ S)-l-(4-bromophenvn-2-rtert-butyl(dimethvnsilylloxy-ethyll-2-methyl- propane-2-sulfinamide
• Step 2 Ethyl 2-r4-r(l S)-2-rtert-butyl(dimethvnsilvHoxy-l-rr(S)-tert- butylsulfinyllaminolethyll phenyl] acetate
• Step 3 Ethyl 2-r4-r(l S)-l-amino-2-hvdroxy-ethyllphenyllacetate
• Step 4 Ethyl 2-r4-r(l S)-l-(tert-butoxycarbonylamino)-2-hvdroxy- ethyl 1 phenyl ] acetate
• Step 2 ( ⁇ )-Ethyl 2444(1 S)-l-amino-2-hvdroxy-ethyl1phenyl1propanoate
• Step 1 ( ⁇ )-Teit-butyl (4S)-4-r4-(l-ethoxycarbonylpropynphenyll-2.2-dimethyl- oxazolidine- 3-carboxylate
• Step 2 ( ⁇ )-Ethyl 2-r4-r(l S)-l-amino-2-hvdroxy-ethyllphenyllbutanoate
• Step 1 Teit-butyl (4S)-4-r4-(2-ethoxy-l-methyl-2-oxo-ethvnphenyll-2.2-dimethyl- oxazolidine-3-carboxylate
• Step 2 Ethyl 2-r4-r(l S)-l-amino-2-hvdroxy-ethyl1phenyl1-2-methyl-propanoate
• aqueous phase was then basified with sodium bicarbonate, and extracted with ethyl acetate (2 X 10 mL). The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated to give the title compound that was used crude in the next step.
• Step 1 Tert-butyl (4S)-4-r4-(l-ethoxycarbonylcvclopropynphenyl1-2.2-dimethyl- oxazolidine-3- carboxylate
• Step 2 Ethyl l-[4-[(l S)-l-amino-2-hydroxy-ethyllphenyllcyclopropanecarboxylate
• Step 1 ( ⁇ )-Teit-butyl (4S)-4-r4-(l-ethoxycarbonyl-2-methyl-propynphenyll-2.2- dimethyl-oxazo- lidine-3-carboxylate
• Step 2 ( ⁇ )-Ethyl 2-r4-r(l S)-l-amino-2-hvdroxy-ethyllphenyll-3-methyl-butanoate
• Example 1 (Method 1) - ( ⁇ ) - N-[3-(acetylsulfamoylamino)-l-phenyl-propyll-4, 5-dichloro- l-methyl-indole-2-carboxamide
• Step 1 ( ⁇ )- Tert-butyl N-r3-r(4.5-dic oro-l-metfayl-indole-2-carbonyl)amino1- 3- pheny 1-propyl ] carb am ate
• Step 2 ( ⁇ )-N-(3-amino-l-phenyl-propyn-4.5-dichloro-l-methyl-indole-2- carboxamide
• Step 3 ( ⁇ ) - N-[3-(acetylsulfamoylamino)-l-phenyl-propyl "
• Example 2 N-[3-facetylsulfamoylamino)propyll-4,5-dichloro-l-methyl-indole-2- carboxamide
• N-[3-(acetylsulfamoylamino)propyl]-4,5-dichloro-l-methyl-indole-2-carboxamide was synthesized via Method 1 with acid A and tert-butyl N-(3-aminopropyl)carbamate (CAS# 75178-96-0).
• HCl in methanol was used for deprotection instead of TFA.
• the final residue was purified by pre-HPLC (Condition: 10 mM NHtHCCb-MeOH; Column: Welch Ultimate AQ-C18 150*30 mm; Particle size: 5 urn) to give the title compound.
• N-[3-[acetylsulfamoyl(methyl)amino]propyl]-4,5-dichloro-l-methyl-indole-2- carboxamide was synthesized via Method 1 with acid A and tert-butyl N-(3-aminopropyl)-N- methyl-carbamate (CAS# 150349-36-3).
• HCl in ethyl acetate was used for deprotection instead of TFA.
• the final residue was purified by prep-HPLC (Condition: 0.225%FA-ACN Column: Phenomenex Synergi Max-RP 250*80 lOu) to give the title compound.
• Example 4 - f ⁇ )-3-[3-fAcetylsulfamoylamino)-l-[f4,5-dichloro-l-methyl-indole-2 - carbonvDaminol propyl] benzoic acid
• Example 5 ( ⁇ VN- ⁇ 3-( acetylsulf amoylamino)- 1- [3- fhvdroxymethyl)phenyl]propyl1-4,5-dichloro -l-methyl-indole-2-carboxamide
• Example 6- 4-[3-fAcetylsulfamoylamino)-l-[H.,5-dichloro-l-methyl-indole-2- carbonvDaminol propyllbenzoic acid
• Example 7 - f ⁇ V4-[3-[AcetylsulfamovUmethvnaminol-l-[f4,5-dichloro-l-methyl- indole-2-carbonyl)- aminolpropyllbenzoic acid
• Example 8 (Method 2) - 4-raS -[[5-Chloro-6-r2-q-hvdroxyethylaminoV2-oxo- ethoxyl-lH-indole-2-carbonyll aminol-2-hydroxy-ethyll benzoic acid
• Step 1 Methyl4-r(l S)-l-rr6-r2-r2-rtert-butyl(dimethvnsilylloxyethylaminol-2-oxo- ethoxyl-5- chloro-lH-indole-2-carbonyl1amino1-2-hvdroxy-ethyl1benzoate
• Step 2 4-r(l S)-l-rr5-Chloro-6-r2-(2-hvdroxyethylamino)-2-oxo-ethoxyl-lH-indole- 2-carbonyll aminol-2-hydroxy-ethyl "
• aStep 1 was run at rt for 12 hours.
• Step 2 was run at rt for 5 hours.
• b Step 1 was performed at rt for 12 hrs.
• c Step 2 was performed at rt for 12 hrs.
• d Steps 1 & 2 were run at rt for 12 hrs.
• e Steps 1 & 2 were run at rt for 1 hr.
• f Step 1 was run at rt for 2 hrs & Step 2 was run at rt for 12 hrs.
• Example 21 4-r S)-l-rr4,5-Dichloro-6-r2- -hvdroxyethylamino)-2-oxo-ethoxyl- l-methyl-indole-2- carbonyllaminol-2-hvdroxy-ethyll benzoic acid
• Example 22 (Method 3) - ( ⁇ V4-q-(4,5-Dichloro-l-methyl-lH-indole-2- carboxamido)-2,2,2-trifluoroethyl)benzoic acid
• Step 1 ( ⁇ )-Methyl 4-(l-(4.5-dichloro-l-methyl-lH-indole-2-carboxamido)-2.2.2- trifluoroethyl) benzoate
• Step 2 ( ⁇ )-4-(l-(4.5-Dichloro-l-methyl-lH-indole-2-carboxamido)-2.2.2- trifluoroethyObenzoic acid
• Step 2 was run at 50 °C for 4 hrs.
• Step 2 was run at 60 °C for 24 hrs.
• c Step 2 was run at 60 °C for 16 hrs.
• d Step 2 was not performed, no hydrolysis necessary.
• e Step 1 was run at 0 °C for 1 hr.
• f Step 1 was run at rt for 1 hr.
• g Step 2 was run at rt for 24 hrs.
• h Step 1 was run at rt for 1 hr.
• Step 2 was run at 90 °C for 12 hrs.
• 'Steps 1 & 2 were run at rt for 1 hr.
• j was run at rt for 1 hr & Step 2 was run at rt for 12 hrs.
• Example 48 ( ⁇ )- 2-(4-q-(4,5-Dichloro-l-methyl-lH-indole-2- carboxamido)ethyl)phenyl) acetic acid

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