WO2017146504A1 - Nécessaire de fourniture d'anticorps, timbre de stockage d'anticorps, dispositif et procédé de diagnostic d'immunité l'utilisant - Google Patents

Nécessaire de fourniture d'anticorps, timbre de stockage d'anticorps, dispositif et procédé de diagnostic d'immunité l'utilisant Download PDF

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Publication number
WO2017146504A1
WO2017146504A1 PCT/KR2017/002028 KR2017002028W WO2017146504A1 WO 2017146504 A1 WO2017146504 A1 WO 2017146504A1 KR 2017002028 W KR2017002028 W KR 2017002028W WO 2017146504 A1 WO2017146504 A1 WO 2017146504A1
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WO
WIPO (PCT)
Prior art keywords
patch
antibody
plate
target protein
reaction
Prior art date
Application number
PCT/KR2017/002028
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English (en)
Korean (ko)
Inventor
이동영
임찬양
김경환
Original Assignee
노을 주식회사
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020160069937A external-priority patent/KR20170099738A/ko
Application filed by 노을 주식회사 filed Critical 노을 주식회사
Priority to EP17756842.5A priority Critical patent/EP3428642B1/fr
Priority to US16/079,451 priority patent/US11385144B2/en
Priority to JP2018562489A priority patent/JP6828986B2/ja
Priority to CA3015599A priority patent/CA3015599C/fr
Priority to EP23172629.0A priority patent/EP4235144A3/fr
Priority to CN201780025367.0A priority patent/CN109073632B/zh
Publication of WO2017146504A1 publication Critical patent/WO2017146504A1/fr
Priority to US17/842,651 priority patent/US20230009655A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/60—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances involving radioactive labelled substances

Definitions

  • the present invention relates to an antibody storage patch, an immunodiagnostic method and apparatus using the same, and more particularly, to a patch storing an antibody and using the same, to perform a diagnosis economically using immunological characteristics from a sample, and to quickly obtain a result.
  • IVD in-vitro diagnosis
  • POCT point-of-care testing
  • Immunohistochemical diagnosis uses antigen-antibody responses and is used for diagnosing and tracking various diseases such as various diseases, cancer markers, and allergies. This has been evaluated as a diagnosis form particularly suitable for on-site diagnosis due to the variety of detectable diseases and the ease of detection. The demand for such immunochemical diagnosis is steadily increasing worldwide, especially in China.
  • One object of the present invention is to provide a patch capable of storing a substance.
  • One object of the present invention is to provide a patch that can provide a reaction space of the material.
  • One object of the present invention is to provide a patch capable of delivering a substance.
  • One object of the present invention is to provide a patch that can absorb a substance.
  • One object of the present invention is to provide a patch that can provide an environment.
  • One object of the present invention is to provide a patch for storing the antibody.
  • One object of the present invention is to provide an immunodiagnostic method using a patch.
  • the immune system includes a net structure for forming microcavities, and performs a diagnosis by detecting a target protein from a sample to be diagnosed using a patch capable of storing a liquid substance in the microcavities.
  • a diagnostic apparatus comprising: a plate support for supporting a plate on which a reaction region is located and a sample to be diagnosed in the reaction region, and a patch for storing an antibody that specifically reacts with the target protein.
  • An immunodiagnostic apparatus may be provided that includes a patch controller for controlling a relative position of a patch to a reaction region to deliver an antibody, and a reaction detector for detecting a specific reaction between the antibody and the target protein to diagnose a target disease. .
  • an antibody that specifically reacts with a target protein, and a mesh structure that forms microcavities in which the antibody is stored, wherein the target protein is in contact with the reaction region where the target protein is located.
  • Antibody storage patches may be provided that include a net construct that delivers a portion to the reaction zone.
  • the antibody that specifically reacts with the target protein may be a primary antibody having specific binding to the target antigen.
  • the antibody that specifically reacts with the target protein may be a secondary antibody having specific binding to an antibody having specific binding to a target antigen.
  • the target protein is an antigen
  • the antibody is an antibody pair formed by binding a primary antibody having specific binding to the antigen and a secondary antibody having specific binding to the primary antibody, wherein the antibody pair is Can specifically react with the antigen.
  • the target protein is plural, and the plurality of target proteins include a first target protein and a second target protein, and the antibody comprises a first antibody and the second target protein that specifically react with the first target protein. It may include a second antibody that specifically reacts.
  • the antibody storage patch comprises an antibody that specifically reacts with a target protein and a mesh structure forming microcavities in which the antibody is stored.
  • a patch cluster may be provided.
  • the plurality of antibody storage patches includes a first antibody storage patch and a second antibody storage patch, and a target protein to which the first antibody specifically stored in the first antibody storage patch is specifically stored in the second antibody storage patch.
  • the second antibody may be different from the target protein to which it specifically reacts.
  • a substrate storage patch comprising a net structure in contact with the reaction region in which the target protein is located to deliver a portion of the stored substrate to the reaction region.
  • a diagnosis is performed by detecting a target protein from a sample to be diagnosed by using a patch including a net structure forming microcavities and storing a liquid substance in the microcavities.
  • An immunodiagnostic method comprising: placing a sample to be diagnosed in a reaction region and delivering the antibody to the reaction region using a patch for storing an antibody that specifically reacts with the target protein, Immune diagnostic methods can be provided.
  • the immunodiagnostic method may further comprise providing the substrate to the reaction zone using a patch that stores the substrate to produce a product through a chemical reaction catalyzed by an enzyme attached to the antibody.
  • the immunodiagnostic method may further include detecting a specific reaction of the antibody and the target protein to diagnose a target disease.
  • the specific reaction may be detected by measuring a change in electrical characteristics of the patch generated by the specific reaction.
  • Detecting the specific reaction includes measuring fluorescence generated by a chemical reaction catalyzed by an enzyme attached to an antibody specifically binding to the target protein, measuring luminescence generated by the chemical reaction or the chemistry. It may be by at least one of the colorimetric measurement caused by the reaction.
  • positioning the sample to be diagnosed is performed by any one of a method of fixing the sample to the plate, a method of plating the sample on the plate or a method of plating the sample on the plate to fix it It may be.
  • Delivering the antibody to the reaction zone using the patch includes contacting the patch with the reaction zone to allow the antibody to move to the reaction zone and separating the patch from the reaction zone.
  • an antibody that does not specifically react with the target protein of the antibody may be removed from the reaction region.
  • the immunodiagnostic method may further include absorbing, from the reaction region, an antibody that does not specifically react with the target protein in the delivered antibody by using a washing patch.
  • Delivering the antibody to the reaction region using the patch delivering the first antibody to the reaction region using a first patch that stores a first antibody that specifically reacts with the target protein. And delivering the second antibody to the reaction region by using a second patch that stores a second antibody that specifically reacts with the first antibody.
  • the reaction region is located on a plate, and a bottom antibody, which is an antibody that specifically reacts with the target protein, is immobilized in the reaction region before the step of placing a sample to be diagnosed in the reaction region.
  • the method may further include providing the plate, wherein positioning the sample to be diagnosed in the reaction region may include placing the sample to be diagnosed in the reaction region to which the bottom antibody is fixed.
  • the target protein is a plurality, wherein the plurality of target proteins include a first target protein and a second target protein, and the patch specifically reacts with the first target protein. And it may be to store a first antibody that specifically reacts with the second target protein.
  • the target protein is a plurality
  • the patch for storing the antibody is a plurality
  • the plurality of target proteins comprises a first target protein and a second target protein
  • the plurality of patches are the agent
  • the first patch may store a first antibody that specifically reacts with the first target protein
  • the second patch may store a second antibody that specifically reacts with the second target protein.
  • a diagnosis is performed by detecting a target protein from a sample to be diagnosed by using a patch including a net structure forming microcavities and storing a liquid substance in the microcavities.
  • An immunodiagnosis method comprising: placing a sample to be diagnosed in a reaction region, and delivering the first antibody to the reaction region by using a patch that stores a first antibody that specifically reacts with a first target protein. And delivering the second antibody to the reaction region using a patch that stores a second antibody that specifically reacts with a second target protein.
  • the step of detecting the first target protein and the second target protein may be further included.
  • the first target protein is detected by detecting a first fluorescence detected from a fluorescent label attached to the first antibody specifically bound to the first target protein, and detecting the second target protein.
  • the method may include detecting a second fluorescence detected from a fluorescent label attached to the second antibody specifically bound to the second target protein.
  • the wavelength band where the first fluorescence is detected may be different from the wavelength band where the second fluorescence is detected.
  • the immunodiagnostic method in the above embodiment further includes detecting the first target protein after delivering the first antibody to the reaction region, and delivering the second antibody to the reaction region. After the step, the method may further include detecting the second target protein.
  • the first target protein is detected by detecting a first fluorescence detected from a fluorescent label attached to the first antibody specifically bound to the first target protein, and detecting the second target protein.
  • the method may include detecting a second fluorescence detected from a fluorescent label attached to the second antibody specifically bound to the second target protein.
  • the wavelength band in which the first fluorescence is detected overlaps at least a portion of the wavelength band in which the second fluorescence is detected, and the detecting of the second fluorescence is performed after delivering the second antibody to the reaction region.
  • the fluorescence detected from the sample may be compared with the fluorescence detected from the sample before delivering the second antibody to the reaction region.
  • an antibody that specifically reacts with a target protein comprises a stored medium and a net structure forming microcavities, in contact with the medium to absorb a portion of the antibody stored in the medium
  • An antibody delivery kit may be provided comprising an antibody delivery patch that contacts a reaction region in which a target protein is located to deliver at least a portion of the absorbed antibody to the reaction region.
  • a diagnosis is performed by detecting a target protein from a sample to be diagnosed by using a patch including a net structure that forms microcavities and capable of handling a liquid substance in the microcavities.
  • An immunodiagnostic method comprising the steps of contacting a medium having stored therein an antibody that specifically reacts with a target protein with the patch and contacting the patch with a reaction region where the target protein is located, wherein the medium is contacted with the patch.
  • an immunodiagnostic method may be provided in which at least a portion of the antibody stored in the medium is absorbed into the patch. In this case, when the patch contacts the reaction region, at least a portion of the antibody absorbed by the patch may be movable to the reaction region.
  • Contacting the medium to the patch is to contact one surface of the medium to the patch, and contacting the patch to the reaction region is to contact one surface that is not in contact with the medium of the patch to the reaction region. It may be.
  • reaction zone of a substance it is possible to provide a reaction zone of a substance or to provide a predetermined environment in a target zone.
  • the immunodiagnosis can be performed more simply, and the diagnosis result can be obtained quickly.
  • the delivery and absorption of the substance is properly controlled using the patch, so that the amount of the solution required for diagnosis can be significantly reduced.
  • a plurality of targets can be detected at the same time and a diagnosis can be performed. Accordingly, a patient-specific diagnosis can be performed.
  • FIG. 1 illustrates in detail an example of a patch according to the present application.
  • FIG. 2 shows an example of a patch according to the present application in detail.
  • FIG 3 illustrates providing a reaction space as an example of the function of a patch according to the present application.
  • FIG. 4 illustrates providing a reaction space as an example of the function of a patch according to the present application.
  • FIG. 5 illustrates the delivery of a substance as an example of the function of a patch according to the present application.
  • FIG. 6 illustrates the delivery of a substance as an example of the function of a patch according to the present application.
  • FIG. 7 illustrates the delivery of a substance as an example of the function of a patch according to the present application.
  • FIG. 8 illustrates the delivery of a substance as an example of the function of a patch according to the present application.
  • FIG 9 illustrates the delivery of a substance as an example of the function of a patch according to the present application.
  • FIG. 10 illustrates the delivery of a substance as an example of the function of a patch according to the present application.
  • FIG. 11 illustrates the delivery of a substance as an example of the function of a patch according to the present application.
  • FIG. 12 illustrates the delivery of a substance as an example of the function of a patch according to the present application.
  • Figure 13 illustrates the delivery of material as an example of the function of the patch according to the present application.
  • FIG. 14 illustrates absorbing material as an example of the function of a patch according to the present application.
  • FIG. 16 illustrates absorbing material as an example of the function of a patch according to the present application.
  • FIG 17 illustrates absorbing material as an example of the function of a patch according to the present application.
  • 21 illustrates absorbing material as an example of the function of a patch according to the present application.
  • FIG. 22 illustrates absorbing material as an example of the function of a patch according to the present application.
  • 23 illustrates an example of providing an environment as one of the functions of a patch according to the present application.
  • FIG. 24 illustrates providing an environment as an example of the functionality of a patch according to the present application.
  • 25 illustrates providing an environment as an example of the functionality of a patch according to the present application.
  • FIG. 26 illustrates a case in which absorption and delivery of a material are performed as an embodiment of a patch according to the present application.
  • FIG. 27 illustrates a case of performing absorption and delivery of a material as an embodiment of a patch according to the present application.
  • FIG. 28 illustrates a case of performing absorption and delivery of a material as an embodiment of a patch according to the present application.
  • 29 is a view illustrating a case of performing absorption and delivery of a material as an embodiment of a patch according to the present application.
  • FIG. 30 illustrates a case of performing absorption and delivery of a material as an embodiment of a patch according to the present application.
  • FIG. 31 illustrates a case in which absorption, delivery of materials, and provision of an environment are performed as an embodiment of a patch according to the present application.
  • 32 is a view illustrating a case of performing absorption, delivery, and provision of an environment as an embodiment of a patch according to the present application.
  • 33 illustrates an embodiment of a plurality of patches as an embodiment of a patch according to the present application.
  • FIG. 34 illustrates an embodiment of a plate having a plurality of patches and a plurality of target areas as one embodiment of a patch according to the present application.
  • 35 is a flowchart illustrating an example of an immunodiagnostic method according to the present application.
  • 36 is a flowchart illustrating an example of an immunodiagnostic method according to the present application.
  • FIG. 37 is a flowchart illustrating an example of an immunodiagnostic method according to the present application.
  • 38 is a flowchart illustrating an example of delivering an antibody to a reaction region in an immunodiagnostic method according to an embodiment of the present application.
  • 39 is a flowchart illustrating an example of an immunodiagnostic method according to the present application.
  • 40 is a flowchart illustrating an example of delivering an antibody to a reaction region in an immunodiagnostic method according to an embodiment of the present application.
  • 41 is a flowchart illustrating an immunodiagnostic method by sandwich ELISA as an example of an immunodiagnostic method according to the present application.
  • FIG. 43 is a flowchart illustrating an immunodiagnostic method by indirect ELISA as an example of an immunodiagnostic method according to the present application.
  • 44 is a flowchart illustrating an example of an immunodiagnostic method according to the present application.
  • 50 illustrates a part of an immunodiagnostic method by indirect ELISA as an embodiment of the immunodiagnostic method according to the present application.
  • 51 shows a part of an immunodiagnostic method by indirect ELISA as an embodiment of the immunodiagnostic method according to the present application.
  • FIG. 52 shows a part of an immunodiagnostic method by indirect ELISA as an embodiment of the immunodiagnostic method according to the present application.
  • FIG. 53 illustrates a part of an immunodiagnostic method by indirect ELISA as an embodiment of the immunodiagnostic method according to the present application.
  • FIG. 54 illustrates washing using a washing patch according to an embodiment of the present application.
  • 55 is a diagram illustrating washing using a washing patch according to an embodiment of the present application.
  • 57 is a flowchart illustrating an immunodiagnostic method by direct ELISA as an example of an immunodiagnostic method according to the present application.
  • FIG. 58 shows a part of the immunodiagnostic method by direct ELISA as an embodiment of the immunodiagnostic method according to the present application.
  • FIG. 59 shows a part of the immunodiagnostic method by direct ELISA as an embodiment of the immunodiagnostic method according to the present application.
  • 60 shows a part of the immunodiagnostic method by direct ELISA as an embodiment of the immunodiagnostic method according to the present application.
  • 61 shows a part of the immunodiagnostic method by direct ELISA as an embodiment of the immunodiagnostic method according to the present application.
  • FIG. 62 shows a part of the immunodiagnostic method by direct ELISA as an embodiment of the immunodiagnostic method according to the present application.
  • 63 shows a patch for storing antibody pairs as one embodiment of a patch according to the present application.
  • 64 shows a patch for storing primary and secondary antibodies as an embodiment of a patch according to the present application.
  • 65 shows an example of a process of manufacturing a patch storing an antibody pair as one embodiment of the patch according to the present application.
  • 66 illustrates an example of a process of fabricating a patch storing antibody pairs as one embodiment of the patch according to the present application.
  • 67 is a flowchart illustrating an immunodiagnostic method by sandwich ELISA as an example of an immunodiagnostic method according to the present application.
  • FIG. 68 illustrates a part of an immunodiagnostic method by sandwich ELISA as an example of an immunodiagnostic method according to the present application.
  • FIG. 69 illustrates a part of an immunodiagnostic method by sandwich ELISA as an example of an immunodiagnostic method according to the present application.
  • FIG. 70 illustrates a part of an immunodiagnostic method by sandwich ELISA as an example of an immunodiagnostic method according to the present application.
  • 71 illustrates a part of an immunodiagnostic method by sandwich ELISA as an example of an immunodiagnostic method according to the present application.
  • FIG. 72 illustrates a part of an immunodiagnostic method by sandwich ELISA as an example of an immunodiagnostic method according to the present application.
  • 73 illustrates a part of an immunodiagnostic method by sandwich ELISA as an example of an immunodiagnostic method according to the present application.
  • 74 shows a patch for absorbing and storing an antibody as an example of a patch of an immunodiagnostic method according to the present application.
  • 75 illustrates a patch for absorbing and storing an antibody as an example of a patch according to the present application.
  • 76 shows, as an example of a patch according to the present application, a patch for absorbing and storing antibodies.
  • 77 shows, as an example of a patch according to the present application, a patch for absorbing, storing and delivering antibodies.
  • 79 illustrates an example of a patch controller in an embodiment of an immunodiagnostic device according to the present application.
  • 80 is a flowchart illustrating an example of an immunodiagnostic method according to the present application.
  • 81 is a flowchart illustrating an embodiment of an immunodiagnostic method according to the present application.
  • 82 is a flowchart illustrating an embodiment of an immunodiagnostic method according to the present application.
  • 83 illustrates a part of cases where a plurality of target proteins are detected in the immunodiagnostic method according to the present application.
  • FIG. 84 illustrates a part of cases where a plurality of target proteins are detected in the immunodiagnostic method according to the present application.
  • the liquid material may mean a material in a liquid state as a material capable of flowing.
  • the liquid phase material may be a single component material having liquidity.
  • the liquid substance may be a mixture including a plurality of substances.
  • the liquid substance when the liquid substance is a substance of a single component, the liquid substance may be a substance composed of a single element or a compound including a plurality of chemical elements.
  • the liquid substance When the liquid substance is a mixture, some of the plural components of the substance may function as a solvent and others may function as a solute. That is, the mixture may be a solution.
  • the material of the plurality of components constituting the mixture may be uniformly distributed.
  • the mixture including the plurality of components may be a mixture mixed uniformly.
  • the material of the plurality of components may include a solvent and a material which is not dissolved in the solvent and is uniformly distributed.
  • the non-uniformly distributed material may also include a particle component that is non-uniformly distributed in the solvent.
  • the heterogeneously distributed particle component may be a solid phase.
  • a material that can be handled using the patch may be in the form of 1) a single component liquid, 2) a solution, or 3) a colloid, and in some cases 4) solid particles are unevenly distributed in other liquid materials. It may be in a state where it is.
  • FIGS. 1 and 2 are diagrams showing an example of a patch according to the present application.
  • a patch according to the present application will be described with reference to FIGS. 1 and 2.
  • the patch PA may include a net structure NS and a liquid material.
  • the liquid substance may be considered by dividing the base material (BS) and the additive material (AS).
  • the patch PA may be a gel type.
  • the patch PA may be implemented as a structure on a gel in which colloidal molecules are bonded to form a net tissue.
  • the patch PA according to the present application may include a three-dimensional net structure NS as a structure for handling the liquid material SB.
  • the net structure NS may be a solid structure that is continuously distributed.
  • the mesh structure NS may have a mesh structure in which a plurality of fine threads are entangled.
  • the mesh structure NS is not limited to the shape of a network in which a plurality of fine threads are entangled, and may be implemented in any three-dimensional matrix form formed by connecting a plurality of fine structures.
  • the net structure NS may be a framework including a plurality of micro-cavities. In other words, the mesh structure NS may form a plurality of fine cavities MC.
  • the net structure of the patch PA may have a sponge structure SS.
  • the net structure of the sponge structure SS may include a plurality of fine holes (MH).
  • MH fine holes
  • the micropores and the microcavities MC may be used interchangeably with each other, and unless otherwise stated, the microcavities MC are defined as including the concept of the micropores MH.
  • the net structure NS may have a regular or irregular pattern.
  • the net structure NS may include both an area having a regular pattern and an area having an irregular pattern.
  • the density of the mesh structure NS may have a value within a predetermined range.
  • the predetermined range may be determined within a limit in which the shape of the liquid substance SB captured in the patch PA is maintained in a form corresponding to the patch PA.
  • the density may be defined as the density of the net structure NS to the mass ratio, the volume ratio, etc. of the net structure NS in the patch.
  • the patch according to the present application can handle the liquid substance (SB) by having a three-dimensional network structure.
  • the patch PA according to the present application may include a liquid material SB, and the liquid material SB included in the patch PA is in the form of the net structure NS of the patch PA.
  • the fluidity of the liquid material (SB) may be limited.
  • the liquid substance SB may freely flow in the net structure NS.
  • the liquid material SB is located in a plurality of microcavities formed by the mesh structure NS. Exchange of the liquid materials SB may occur between neighboring microcavities.
  • the liquid material (SB) may be present in a form that penetrates the frame structure forming the net structure. In such a case, nano-sized pores may be formed in the frame structure to allow the liquid material SB to penetrate.
  • the molecular weight of the liquid material (SB) trapped in the patch (PA) to the size of the particles it can be determined whether the liquid material (SB) to the frame structure of the mesh structure.
  • a material having a relatively high molecular weight may be trapped in the microcavity, and a material having a relatively low molecular weight may be injected into the microcavity and / or the frame structure of the mesh structure NS to be captured.
  • the term “capture” refers to a state in which the liquid substance SB is located in a plurality of fine cavities and / or the nano-sized holes formed by the mesh structure NS. Can be defined in addition, the state in which the liquid substance SB is trapped in the patch PA, as described above, the liquid substance SB may flow between the microcavity and / or the nano-sized holes. It is defined to include the state that exists.
  • the liquid material SB may be considered as being divided into a base material BS and an additive material AS as follows.
  • the base material BS may be a liquid material SB having fluidity.
  • the additive material AS may be a material mixed with the base material BS and having fluidity.
  • the base material BS may be a solvent.
  • the additive material AS may be a solute dissolved in the solvent or particles insoluble in the solvent.
  • the base material BS may be a material that may flow in the matrix formed by the net structure NS.
  • the base material (BS) may be uniformly distributed in the net structure (NS), may be distributed only in a portion of the net structure (NS).
  • the base material BS may be a liquid having a single component.
  • the additive material AS may be a material mixed with the base material BS or soluble in the base material BS.
  • the additive material AS can function as a solute using the base material BS as a solvent.
  • the additive material AS may be uniformly distributed in the base material BS.
  • the additive material AS may be minute particles that do not dissolve in the base material BS.
  • the additive material (AS) may contain microparticles such as colloidal molecules and microorganisms.
  • the additive material AS may include particles larger than the microcavities formed by the net structure NS. If the size of the microcavities is smaller than the size of the particles included in the additive material AS, the fluidity of the additive material AS may be limited.
  • the additive material AS may include a component that is selectively included in the patch PA.
  • the additive material AS does not necessarily mean a material that is inferior in quantity or functionally inferior in relation to the base material BS described above.
  • the property of the liquid material SB captured in the patch PA may be regarded as the property of the patch PA. That is, the characteristics of the patch PA may depend on the properties of the material trapped in the patch PA.
  • the patch PA according to the present application may include the net structure NS as described above.
  • the patch PA may handle the liquid substance SB by the mesh structure NS.
  • the patch PA may allow the liquid substance SB trapped in the patch PA to maintain at least some of its own characteristics.
  • the diffusion of the material may occur in a region of the patch PA in which the liquid material SB is distributed, and a force such as surface tension may act.
  • the patch PA may provide a liquid environment in which a target material is diffused due to thermal movement, density, or concentration difference of the material.
  • 'diffusion' means that the particles that make up a substance are spread from the higher concentration to the lower concentration due to the difference in concentration.
  • These diffusion phenomena can be understood basically as the resulting phenomena caused by the movement of molecules (translational movements in gases or liquids, vibrational movements in solids, etc.).
  • the term 'diffusion' refers to a phenomenon in which particles are spread from a high concentration to a low concentration due to a difference in concentration or density.
  • the phenomenon of movement of particles by irregular motion is also referred to.
  • the target material to be diffused may be a solute dissolved in the liquid material (SB), and the solute may be provided in a solid, liquid, or gaseous state.
  • non-uniformly distributed material in the liquid material SB captured by the patch PA may be diffused in the space provided by the patch PA.
  • the additive material AS may diffuse in the space defined by the patch PA.
  • the non-uniformly distributed material or the additive material AS of the liquid material SB handled by the patch PA diffuses in the microcavities provided by the mesh structure NS of the patch PA. can do.
  • the region in which the non-uniformly distributed material or the additive material AS may diffuse may be changed by contacting or connecting another material with the patch PA.
  • the material or the additive material AS may constantly move due to irregular movement of molecules in the interior of the patch PA and / or in the external region connected with the patch PA.
  • the patch PA may be implemented to have hydrophilic or hydrophobic properties.
  • the net structure NS of the patch PA may be hydrophilic or hydrophobic.
  • the net structure NS may handle the liquid material SB more effectively.
  • the base material BS may be a hydrophilic material having polarity or a hydrophobic material having no polarity.
  • the nature of the additive material (AS) may be hydrophilic or hydrophobic.
  • the nature of the liquid substance SB may be related to the base substance BS and / or the additive substance AS.
  • the liquid material SB may be hydrophilic
  • both the base material BS and the additive material AS may be hydrophilic
  • the liquid material (SB) may be hydrophobic
  • the polarities of the base material BS and the additive material AS are different from each other, the liquid material SB may be hydrophilic or hydrophobic.
  • both the polarity of the net structure NS and the polarity of the liquid material SB are hydrophilic or hydrophobic, an attractive force may act between the net structure NS and the liquid material SB.
  • the polarities of the net structure NS and the liquid material SB are opposite to each other, for example, when the polarity of the net structure NS is hydrophobic and the liquid material SB is hydrophilic.
  • the repulsive force may act between the net structure NS and the liquid material SB.
  • the patch PA may be used alone, in plurality, or in combination with other media to induce a desired reaction.
  • the functional aspects of the patch PA will be described.
  • the patch PA is a gel phase that may contain a hydrophilic solution.
  • the mesh structure NS of the patch PA is assumed to have hydrophilic properties.
  • Patches according to the present application may have some useful functionality, due to the properties described above.
  • the patch may be involved in the behavior of the liquid material SB by occupying the liquid material SB.
  • the reservoir function and the state of the material in which the state of the material is defined in a predetermined region formed by the patch PA according to the behavior of the material in relation to the patch PA are described.
  • the channeling function in which the state of the material is defined including an external region will be described.
  • the patch PA according to the present application may capture the liquid substance SB as described above.
  • the patch PA may function as a reservoir.
  • the patch PA may capture a liquid material SB in a plurality of microcavities formed in the mesh structure NS through the mesh structure NS.
  • the liquid material SB occupies at least a portion of the microcavities formed by the three-dimensional network structure NS of the patch PA, or a nano-sized hole formed in the network structure NS. Can penetrate
  • the liquid substance SB located in the patch PA does not lose the property of the liquid even if it is distributed in the plurality of microcavities. That is, the liquid substance SB has fluidity even in the patch PA, and the diffusion of the substance may occur in the liquid substance SB distributed in the patch PA, and an appropriate solute may be dissolved in the substance. have.
  • the patch PA may capture a target material based on the above-described characteristics.
  • the patch PA may be resistant to a change in the external environment within a predetermined range. Through this, the patch PA may keep the material in the captured state.
  • the liquid substance SB which is the target of the capture, may occupy the three-dimensional network structure NS.
  • the meaning that the patch PA stores the liquid substance means that the liquid substance is stored in the space formed by the mesh structure and / or to the frame structure constituting the mesh structure NS. It is defined as encompassing all that the liquid substance is stored.
  • the patch PA may store a liquid material SB.
  • the patch PA may store the liquid substance SB.
  • the liquid material SB may be stored in combination with the net structure NS with a attraction force of a predetermined intensity or more.
  • the properties of the liquid material SB stored in the patch PA may be classified according to the properties of the patch PA. More specifically, when the patch PA is hydrophilic, the hydrophilic liquid SB is combined with a polar hydrophilic liquid SB to form the three-dimensional fine particles. Can be stored in cavities. Alternatively, when the patch PA is hydrophobic, the hydrophobic liquid material SB may be stored in the microcavity of the three-dimensional network structure NS.
  • the amount of material that can be stored in the patch PA may be proportional to the volume of the patch PA.
  • the amount of material stored in the patch PA may be proportional to the amount of the three-dimensional network structure NS as a support contributing to the shape of the patch PA.
  • the volume relationship between the amount of the material that can be stored and the volume of the patch PA does not have a constant proportional constant, and the amount of the material that can be stored and the volume of the patch PA according to the design or manufacturing method of the mesh structure. Relationships can vary.
  • the amount of material stored in the patch PA may be reduced by evaporation, dropping, etc. over time.
  • a substance to the patch (PA) it can increase or maintain the content of the substance stored in the patch (PA).
  • a moisture preservative for suppressing evaporation of moisture may be added to the patch PA.
  • the patch PA may be embodied in an easy form for storing the liquid material SB. This means that the patch PA may be implemented to minimize the degeneration of the material when the material is affected by environment such as humidity, light quantity, temperature, and the like. For example, in order to prevent the patch PA from being denatured by an external factor such as bacteria, the patch PA may be treated with a bacterial inhibitor or the like.
  • the patch PA may store a liquid material SB having a plurality of components.
  • the material of the plural components is placed together in the patch PA before the reference time point, or the material injected into the patch PA is first stored in the patch PA first, and then the secondary material is secondary to the patch PA after a predetermined time.
  • the substance it is also possible for the substance to be stored.
  • two components of the liquid substance SB are stored in the patch PA, two components are stored in the patch PA or two components are produced in the patch PA. Only one component may be stored in the patch PA and the other one may be stored later, or two components may be sequentially stored after fabrication of the patch PA.
  • the material stored in the patch PA may exhibit fluidity basically, and may also perform irregular or diffusion motion by molecular motion in the patch PA.
  • 3 and 4 are diagrams for providing a reaction space as an example of the function of the patch according to the present application.
  • the patch PA according to the present application may perform a function of providing a space.
  • the patch PA may provide a space in which the liquid material SB may move through a space formed by the net structure NS and / or a space constituting the net structure NS. have.
  • the patch PA may provide space for activities other than the diffusion of particles and / or irregular movement of the particles (hereinafter referred to as activities other than diffusion). Activities other than diffusion may refer to chemical reactions, but are not limited thereto and may also mean physical state changes. More specifically, activity other than diffusion means a chemical reaction in which the chemical composition of the substance changes before and after the activity, a specific binding reaction between components included in the substance, and a solute or particle contained in the substance and distributed unevenly. Homogenization, aggregation of some components contained in the material, or biological activity of a portion of the material.
  • the plurality of substances when a plurality of substances are involved in the activity, the plurality of substances may be located together in the patch PA before the reference time point.
  • the plurality of materials may be sequentially added.
  • the efficiency of the function of providing a space for activities other than the diffusion of the patch PA can be enhanced.
  • the temperature conditions of the patch PA may be changed or electrical conditions may be added to facilitate the activity or to initiate the activity.
  • the first material SB1 and the second material SB2 positioned in the patch PA react with the inside of the patch PA to be transformed into a third material SB3, or
  • the third material SB3 may be generated.
  • Movement of material may occur between the patch PA and the outer region.
  • the material may be moved from the patch PA to the outer region of the patch PA, or the material may be moved from the outer region to the patch PA.
  • the patch PA may form a path of movement of the material or may be involved in the movement of the material. More specifically, the patch PA is involved in the movement of the liquid substance SB trapped in the patch PA or through the liquid substance SB trapped in the patch PA. May be involved in the movement
  • the base material BS or the additive material AS may exit from the patch PA, or an external material may flow into the patch PA from an external region.
  • the patch PA may provide a function of the movement passage of the material. That is, the patch PA may provide a channel function of material movement by participating in material movement. The patch PA may provide a channel of mass movement due to the inherent property of the liquid substance SB.
  • the patch PA may be in a state in which the liquid substance SB may move between the outer region or the outer region, depending on whether the patch PA is connected to the outer region. ) May be in a state where it is impossible to move.
  • the patch PA may have unique functions.
  • the basic reason why the movement of the liquid material SB occurs is due to the irregular movement and / or diffusion of the material.
  • external environmental factors eg, control of temperature conditions, control of electrical conditions, etc.
  • the liquid substance SB or some components of the liquid substance SB may diffuse into the outer region or move by irregular movement.
  • the foreign substance or some component of the foreign substance located in the outer region may diffuse into the liquid substance SB of the patch PA or move by irregular movement.
  • the state in which the substance is movable may be caused by contact.
  • the contact may mean that the liquid material SB captured in the patch PA is connected to the external region.
  • the contact may mean that the flow region of the liquid material SB overlaps at least part of the outer region.
  • the contact may mean that the external material is connected to at least a portion of the patch PA.
  • the state in which the substance is movable may be understood as the range in which the captured liquid substance SB flows is expanded. In other words, in a state in which the substance is movable, the liquidity can be extended so that the flowable range of the substance includes at least a portion of the outer region of the captured liquid substance SB.
  • the range in which the captured liquid material SB is flowable may be extended to include at least a portion of the contacted outer region. More specifically, when the outer region is an outer plate, the region in which the liquid substance SB is flowable may be expanded to include a region in contact with the liquid substance SB of the outer plate.
  • movement of the material may not occur between the liquid material SB captured in the patch PA and the external region.
  • the movement of the material may occur in each of the liquid material SB captured in the patch PA and the external material located in the external region.
  • the state in which the material is not movable may be a state in which the contact is released.
  • the liquid material SB remaining in the patch PA and the outer region or the outer substance may not move. .
  • the contact released state may mean a state in which the liquid material SB captured in the patch PA is not connected to the external region.
  • the contact released state may mean a state in which the liquid material SB is not connected to an external material located in the external region.
  • a state in which the movement of the material is impossible may be caused by separation of the patch PA and the external region.
  • movable state as defined herein has a meaning distinguished from “non-movable state”, but transition between states may occur due to the passage of time, the environment, and the like.
  • the patch PA may be in a movable state and may be in a non-movable state, may be in a non-movable state and may be in a movable state, and the patch PA may be in a movable state and then may not be moved. It is also possible to move back to a ready state.
  • the patch PA may transmit at least a portion of the liquid material SB occupied by the patch PA to the desired outer region due to the above-described characteristics.
  • the delivery of the substance may mean that a part of the liquid substance SB captured in the patch PA is separated from the patch PA as a predetermined condition is satisfied. Partial separation of the liquid substance SB may mean that some substances are extracted, emitted, or released from an area affected by the patch PA. This is a sub-concept of the channel function of the above-described patch (PA), it can be understood to define the delivery (delivery) of the material located in the patch (PA) outside the patch (PA).
  • the desired outer region may be another patch PA, a dried region, or a liquid region.
  • the predetermined condition for the delivery to occur may be determined by environmental conditions such as temperature change, pressure change, electrical property change, physical state change.
  • environmental conditions such as temperature change, pressure change, electrical property change, physical state change.
  • the transfer may include moving the liquid substance SB between the patch PA and the outer region and moving the liquid substance SB between the patch PA and the outer region. It can happen via / through.
  • the liquid substance SB when the liquid substance SB is in the movable state, the liquid substance SB may diffuse between the patch PA and the outer region or may move to the outer region by an irregular movement.
  • the base solution and / or the additive material AS included in the liquid material SB may move from the patch PA to the outer region.
  • movement between the patch PA and the outer region becomes impossible.
  • some of the material that has been moved from the patch PA to the outer region due to the diffusion and / or irregular movement of the liquid material SB is due to the transition from the movable state to the non-movable state. It will not be possible to move back to the patch PA. Therefore, some of the liquid substance SB may be partially transferred to the outer region.
  • the transfer may be performed according to the difference between the attraction force between the liquid substance SB and the net structure NS and the attraction force between the liquid substance SB and the external region or the external substance.
  • the attraction may result from the similarity or specific binding relationship of polarity.
  • the movable state and the non-movable state At least a portion of the liquid material SB captured in the patch PA may be transferred to the outer region through the state.
  • the delivery of the liquid substance SB may optionally be performed. For example, when there is a specific binding relationship between some components included in the liquid substance (SB) and the external substance, the some components pass through the state in which the substance is movable and the state in which the substance cannot be moved. An optional delivery of may occur.
  • the patch PA delivers the material to the outer plate PL in the form of a plate
  • a part of the liquid material SB captured in the patch PA (for example, a solute) A material that specifically binds to) may be applied to the outer plate PL.
  • the patch PA passes through the movable state and the non-movable state, and the part of the solute that specifically binds to the material applied to the outer plate PL is attached to the plate PA. Can optionally be delivered.
  • liquid material SB is transferred from the patch PA to a separate outer plate PL.
  • the case where the material is moved from the patch PA to the plate PL such as slide glass may be considered.
  • the liquid substance SB trapped in the patch PA diffuses into at least a portion of the plate PL or moves by irregular movement. Can be.
  • some material that is, a part of the liquid material SB
  • the partial material may be transferred from the patch PA to the plate PL.
  • the some material to be delivered may be the additive material (AS).
  • the patch PA may be provided with a temperature or electrical condition to control the delivery of the substance.
  • the movement of material from the patch PA to the plate PL may depend on the contact area between the patch PA and the plate PL.
  • the mass transfer efficiency of the patch PA and the plate PL may increase or decrease according to an area where the patch PA contacts the plate PL.
  • the patch PA comprises a plurality of components
  • only some components may be selectively moved to the outer plate PL.
  • a material that specifically binds to some components of the plurality of components may be fixed to the outer plate PL.
  • the material fixed to the outer plate PL may be in a liquid or solid state and may be fixed in the separate area.
  • some materials of the plurality of components move to the plate PL to form a specific bond due to contact between the patch PA and the separate region, and the patch PA is connected to the plate PL.
  • only some components can be selectively released into the plate PL.
  • the patch PA may transfer a part of the material stored in the patch PA to the plate PL by contacting the outer plate PL.
  • the transferring of the material may be enabled to move the material by contacting the plate.
  • the water film WF may be formed near the contact surface between the plate and the patch PA, and the material may be moved through the formed water film WF.
  • the material SL having fluidity may be a liquid material contained in a separate storage space or flowing.
  • the liquid material SB trapped in the patch PA has at least a part of the fluidity.
  • the branch may diffuse and move to the material SL or may move by an irregular motion.
  • some of the liquid material SB, which has been moved from the patch PA to the flowable material cannot move back to the patch PA.
  • some materials in the patch PA may be transferred to the fluid material.
  • Material movement between the patch PA and the flowable material SL may depend on the contact area between the patch PA and the flowable material SL.
  • the patch PA may have fluidity with the patch PA according to an area where the patch PA contacts the fluid material SL (for example, a depth into which the patch PA is injected into a solution or the like).
  • the mass transfer efficiency of the material SL may be increased or decreased.
  • mass transfer between the patch PA and the flowable material SL may be controlled through physical separation of the patch PA and the flowable material.
  • the distribution concentration of the additive material (AS) in the liquid material (SB) is different from the distribution concentration of the additive material (AS) in the flowable material, and thus from the patch (PA) to the flowable material.
  • the additive material AS may also be delivered.
  • the physical separation between the patch PA and the fluid SL is essential. no.
  • the driving force (causal force) that causes the mass movement from the patch (PA) to the fluid having a flow becomes smaller or less than the reference value, the movement of the substance can be stopped.
  • the 'delivery conditions' between the patch PA and the flowable material SL may not be required. It may be. This means that the materials that have already moved to the fluid material SL are moved by diffusion and / or irregular motion in the fluid material SL, and the moving material and the patch PA are moved by the movement. When the distance between them is more than a certain distance it can be understood that the material is transferred to the fluid material (SL). This is because, in the case of the plate PL, since the movable range extended by the contact is a very limited range, the attraction force between the materials moved to the plate PL and the patch PA can act significantly.
  • the patch PA may transfer a part of the material stored in the patch PA to an external fluid material. Delivering a portion of the stored material is that the patch (PA) is put into or in contact with the fluid material, the liquid material (SB) and the fluid material trapped in the patch (PA) of the material This may be achieved by having a state in which the movement is possible.
  • the liquid material SB provided to the patch PA may move to at least a portion of the other patch PA.
  • the liquid substance SB provided to each of the patches PA may diffuse and move to the other patch PA.
  • the concentration of the liquid material (SB) provided in each of the patches (PA) may be changed.
  • the patch PA and the other patch PA may be separated, and at this time, a part of the liquid material SB of the patch PA is different from the patch PA. Can be delivered.
  • Mass transfer between the patch PA and another patch PA can be performed by changes in environmental conditions, including physical state changes.
  • Material movement between the patch PA and the other patch PA may depend on the contact area of the patch PA and the other patch PA.
  • the mass transfer efficiency between the patch PA and the other patch PA may increase or decrease according to an area where the patch PA contacts the other patch PA.
  • 11 to 13 illustrate the delivery of material from one patch PA1 to another patch PA2 as an example of the delivery of material during the function of the patch PA according to the present application.
  • the patch PA1 may transfer a part of the material stored in the patch PA1 to another patch PA2.
  • Delivering a portion of the material is that the patch (PA1) in contact with the other patch (PA2), the liquid material (SB) trapped in the patch (PA1) and the material captured in the other patch (PA2) It can be achieved by having a state in which interchange with each other.
  • 'absorption' of the function of the patch PA may be treated similarly to the 'delivery' described above in some embodiments.
  • the direction of movement of the moved substance can be controlled by changing the concentration of the liquid substance SB, in particular, the concentration of the additive substance AS. It may have a common aspect in that it is.
  • the control of the movement of the material through the separation of the physical contact of the patch (PA), and the like can also be common, which will be clearly understood by those skilled in the art to which the present application belongs.
  • the patch PA may capture an external material by the above-described characteristics.
  • the patch PA may pull external materials existing outside the region defined by the patch PA to a region where the influence of the patch PA acts.
  • the introduced foreign material may be captured together with the liquid material SB of the patch PA.
  • the introduction of the foreign material may be attributable to the attraction between the foreign substance and the liquid substance SB trapped in the patch PA.
  • the introduction of the external material may result from the attraction between the external material and the region not occupied by the liquid material SB of the net structure NS.
  • the ingress of the foreign material may result from the force of the surface tension.
  • absorption is a sub-concept of the channel function of the patch PA described above, and can be understood to define the movement of foreign material to the patch PA.
  • the absorption may occur via (via / through) the patch PA in a state in which the movement of the material and in a state in which the movement of the material is impossible.
  • the material absorbed by the patch PA may be in a liquid or solid state.
  • the liquid material SB located in the patch PA and the solid material included in the external material may be separated from each other. Absorption of the material can be performed with
  • the patch PA when the patch PA is in contact with a liquid external material, the patch PA may be performed by combining the liquid material SB located in the patch PA with the liquid external material.
  • the external material absorbed by the patch PA may move into the patch PA or may be distributed on the surface of the patch PA through a microcavity of the net structure NS forming the patch PA. can do.
  • the distribution position of the foreign material may be determined from the molecular weight of the foreign material or the size of the particles.
  • the shape of the patch PA may be modified while the absorption is performed.
  • the volume, color, etc. of the patch PA may change.
  • external conditions such as temperature change and physical state change may be added to the absorption environment of the patch PA to activate or slow down the absorption of the patch PA.
  • absorption will be described as a function of the patch PA, in accordance with some examples of the outer region providing the material absorbed into the patch PA when absorption occurs.
  • the patch PA absorbs an external material from a separate outer plate PL.
  • the separate external substrate may exemplify a plate PL, etc., in which the external material may be located while not absorbing the external material.
  • a material may be applied to the outer plate PL.
  • the plate PL may be coated with a material in powder form.
  • the material applied to the plate PL may be a single component or a mixture of multiple components.
  • the plate PL may have a flat plate shape.
  • the plate PL may be modified in shape to improve storage properties of the material. For example, it is possible to form a well to improve storage properties, to deform the surface of the plate PL in an engraved or embossed form, or to improve contact with the patch PA by using a patterned plate PL. It may be.
  • Absorption of a material from the plate PL by the patch PA according to the present application may be caused by contact between the plate PL and the patch PA.
  • the water film due to the liquid material SB captured in the patch PA and / or the material applied to the plate PL (WF) can be formed.
  • an aquaplane (WF, aquaplane) is formed in the contact area, the material applied to the plate (PL) can be captured in the water film (WF).
  • the material trapped in the water film WF may freely flow in the patch PA.
  • the water film WF moves along with the patch PA so that the material applied to the plate PL is applied to the patch PL.
  • PA can be absorbed.
  • the material applied to the plate PL may be absorbed into the patch PA as the patch PA is spaced apart from the plate PL by a predetermined distance or more.
  • the liquid substance SB provided to the patch PA does not move to the plate PL, or only a slight amount of the patch PA. Can be absorbed).
  • All or part of the material applied to the plate PL may specifically react with all or part of the material trapped in the patch PA.
  • the absorption of the material from the separate plate PL by the patch PA may be selectively performed. In particular, this may be the case when the patch PA has a stronger attraction force than the plate PL with respect to a part of the material trapped in the patch PA.
  • some materials may be fixed to the plate PL.
  • some materials are fixed to the plate PL and some materials are not fixed or may be applied with fluidity.
  • the patch PA and the plate PL are in contact with and separated from each other, only the material except for the fixed part of the material applied to the plate PL may be selectively absorbed into the patch PA.
  • selective absorption may occur due to the polarity of the material located in the plate PL and the material trapped in the patch PA, regardless of fixation.
  • the patch PA when the liquid material SB captured in the patch PA specifically binds to at least a portion of the material applied to the plate PL, the patch PA may be attached to the plate (P). When contacted with and separated from the material applied to PL), only at least a part of the specifically bound material of the material applied to the plate PL may be absorbed into the patch PA.
  • some of the material applied to the plate PL may specifically react with a material previously fixed to the plate PL. In this case, only the remainder of the material applied to the plate PL may be absorbed into the patch PA except for a material that specifically reacts with a material previously fixed to the plate PL.
  • the patch PA absorbs the material from the outer plate PL.
  • the patch PA may absorb a portion of the material located on the outer plate PL from the outer plate PL.
  • Absorption of the material may include forming a water film WF near a contact area between the outer plate PL and the patch PA by contacting the outer plate PL with the patch PA. This can be achieved by allowing the material to move into the patch PA through WF).
  • the material SL having fluidity may be a liquid external material contained in a separate storage space or flowing. More specifically, the fluid material SL and the liquid material SB trapped in the patch PA have an environment in which they can flow with each other, whereby a part or part of the fluid material SL is present. All may be absorbed into the patch PA. In this case, the mutually flowable environment may be formed by at least partially contacting the patch PA with the fluid SL.
  • the patch PA may be in a state where the material SL and the fluid may move.
  • the patch PA is separated from the flowable material SL, at least a part of the flowable material SL may be absorbed into the patch PA.
  • Absorption of the material into the patch PA from the fluid SL may depend on the concentration difference between the material trapped in the patch PA and the fluid SL.
  • the liquid substance SB trapped in the patch PA is more concentrated in the predetermined additive substance AS than the concentration of the fluid SL in relation to the predetermined additive substance AS.
  • the concentration is low, the predetermined additive material AS may be absorbed into the patch PA.
  • the material when the material is absorbed from the fluid SL to the patch PA, in addition to depending on the concentration difference in the contacted state as described above, by adding an electrical factor or by changing the physical conditions The absorption of the patch PA can be controlled. Furthermore, the material captured by the patch PA and the material to be absorbed may not be directly contacted, but may be indirectly contacted through a medium to absorb the material.
  • the patch PA may absorb a portion of the flowable material SL.
  • Absorption of the material may include a liquid material SB captured by the patch PA by being injected into the material SL having the fluidity or contacting the material SL having the fluidity.
  • the fluid SL may be made to move with each other.
  • Absorption of an external material from the patch PA by the patch PA may include absorption of the external material and the material trapped in the patch PA and the external material and the patch PA.
  • the absorbent material is hydrophilic
  • the patch PA is hydrophilic
  • the attraction force between the absorbed material and the patch PA is the attraction force between the other patch PA and the absorbed material.
  • the patch PA3 may absorb a portion of the material located in the other patch PA4.
  • Absorption of the substance may include the liquid substance SB captured by the patch PA3 and the liquid substance SB captured by the other patch PA4 by contacting the patch PA3 with another patch PA4. ) Can be achieved by interacting with each other.
  • the binding force of the patch PA to the absorbed external material may vary according to the ratio of the total volume of the patch PA of the frame structure of the three-dimensional net structure NS constituting the patch PA. Can be. For example, as the volume ratio of the frame structure to the entire patch PA increases, the amount of the material trapped in the structure may decrease. In this case, the bonding force between the patch PA and the target material may decrease due to a decrease in contact area between the material captured in the patch PA and the target material.
  • the polarity of the patch PA may be controlled by adjusting the proportion of the material forming the net structure NS in the manufacturing step of the patch PA.
  • the degree of absorption may be adjusted by controlling the concentration of the agarose.
  • the separate area has a weak bonding force with respect to the material provided from the patch PA compared to the patch PA, and the patch PA and the other patch PA are contacted and separated, the absorption is performed.
  • the foreign material may be separated from the other patch PA together with the patch PA.
  • the patch PA according to the present application may perform a function of adjusting environmental conditions of a desired region by the above-described characteristics.
  • the patch PA may provide an environment resulting from the patch PA in a desired area.
  • Environmental conditions resulting from the patch PA may depend on the liquid substance SB trapped in the patch PA.
  • the patch PA may create a desired environment for the material located in the outer region so as to correspond to the properties of the material contained in the patch PA or to the properties of the material contained in the patch PA.
  • Adjusting the environment can be understood as changing the environmental conditions of the desired area.
  • the changing of the environmental conditions of the target area may be performed in such a way that the area affected by the patch PA extends to include at least a part of the desired area or the environment of the patch PA with the target area. It may be implemented in a shared form.
  • the provision of the environment by the patch PA may be performed in a state in which the patch PA may move the material and the external area to provide the environment.
  • the provision of the environment by the patch PA can be performed due to the contact. For example, when the patch PA contacts a target area (eg, an external material, a plate PL, etc.), the patch PA may provide a specific environment in the target area. .
  • a target area eg, an external material, a plate PL, etc.
  • the patch PA may provide an environment such as pH, osmotic pressure, humidity, concentration, temperature, and the like to adjust the environment of the target area TA.
  • the patch PA may impart liquidity to the target area TA or the target material. This impartation of fluidity can occur due to some movement of the material trapped in the patch PA.
  • the wetting / moist environment may be provided to the target area TA through the liquid material SB to the base material BS captured by the patch PA.
  • Environmental factors provided by the patch PA may be kept constant according to the purpose.
  • the patch PA may provide homeostasis to the desired area.
  • environmental conditions of the desired area may be adapted to the material captured in the patch PA.
  • Providing an environment by the patch PA may be a result of the diffusion of the liquid material SB included in the patch PA. That is, when the patch PA and the target region contact, the movement of the material may be possible through the contact region formed by the contact.
  • an environmental change due to osmotic pressure, an environmental change due to ion concentration, a wet environment, a change in pH, and the like may be implemented according to the diffusion direction of the material.
  • the patch PA may provide a predetermined environment to the outer plate PL on which the fourth material SB4 and the fifth material SB5 are located.
  • the patch PA may provide a predetermined environment for forming the sixth material SB6 by reacting the fourth material SB4 and the fifth material SB5 to the plate PL. .
  • the water film (WF) is formed in the vicinity of the contact area by the patch (PA) in contact with the plate (PL) and the fourth material (SB4) and the fifth material in the formed water film (WF) (SB5) can be made by being captured.
  • the patch PA according to the present application may be implemented to perform various functions by appropriately applying the functions of the above-described patch PA.
  • the patch PA may provide a reaction zone of a material.
  • the reaction of the material may occur in at least a part of the spatial region affected by the patch PA.
  • the reaction of the substance, the reaction between the liquid substance (SB) trapped in the patch (PA), and / or the substance provided from the outside of the patch (PA) and the liquid substance (SB) trapped. Can be.
  • Providing a reaction zone of the substance may be to activate or promote the reaction of the substance.
  • the liquid substance (SB) trapped in the patch (PA) is a substance introduced at the time of fabrication of the patch (PA), is added to the patch (PA) after fabrication and stored in the patch (PA) At least one of the material being and the material temporarily trapped in the patch (PA).
  • the material is captured in the patch PA at the time when the reaction in the patch PA is activated, it is irrespective of whether it is captured in the patch PA in any form. Can react.
  • a material to be introduced after fabrication of the patch PA to act as a reaction initiator.
  • the provision of the reaction zone of the reaction involving the liquid substance SB trapped in the patch PA may be an exemplary sub-concept of the table of contents described above in 2.1.3 (ie, the provision of the reaction space). Or, it may be a multi-concept that performs the combined functions of the above-listed 2.1.3 and 2.2.4.2 (ie, absorption) tables of contents.
  • the present invention is not limited thereto, and two or more functions may be implemented in a merged form.
  • the absorption function of the patch PA and the provision function of the reaction space are performed by one patch PA.
  • the absorption function and the providing function may be a function that is performed at the same time, may be a function that is performed at different time points, or may be sequentially performed to perform another function.
  • the patch PA further includes not only the absorbing and providing functions but also additional functions.
  • the patch PA may perform a function of capturing a material, and the material may be fluid even when the material is captured. If the distribution of some components of the liquid substance (SB) is non-uniform, the non-uniform components may diffuse. Even when the components of the liquid substance SB are uniformly distributed, the liquid substance SB may be in a state of mobility at a predetermined level due to irregular movement of particles. At this time, a reaction between materials, for example, specific binding between materials, may occur in the patch PA.
  • the fluid having a newly captured fluidity in the patch PA and the material trapped in the patch PA perform specific binding to each other. Form reactions may also be possible.
  • the reaction between the flowable material and the trapped material may be performed separately from any space in which the flowable material has been provided.
  • the patch PA absorbs the flowable material from any space
  • the patch PA is separated from the random space, so that the absorbed material and the patch PA Reaction of the trapped material may occur in the patch PA.
  • the patch PA may perform an absorption function of the fluid material, so that the reaction of the trapped material may occur.
  • a reaction between the absorbed material and the material trapped in the patch PA may occur by triggering the absorption of the fluid material of the patch PA.
  • the reaction may be performed in a space defined by the patch PA.
  • the composition of the liquid material SB captured in the patch PA may be changed.
  • the chemical composition may be changed before and after the reaction.
  • the composition distribution according to the position of the material in the patch PA may be changed. This can be exemplified by diffusion or by particles having specific attractive forces to other materials.
  • the composition of the liquid material SB is changed due to the reaction inside the patch PA, the material outside the patch PA and the patch PA (if there is a contact material, the contacted material). Due to the difference in concentration, some materials may be absorbed into the patch PA, or the materials may be released from the patch PA to the external material.
  • the patch PA may store a material and provide a reaction space of the stored material.
  • the reaction space provided by the patch PA may be a surface area of the microcavity or the patch PA formed by the mesh structure NS of the patch PA.
  • the reaction space may be a surface area of the patch PA.
  • the reaction space provided by the patch PA may serve to provide a specific environmental condition.
  • the patch PA may adjust the environmental conditions of the reaction while the reaction in the liquid substance SB located in the patch PA is in progress.
  • the patch PA can perform the function of a buffer solution.
  • the patch PA stores material through the net structure, and thus does not require a separate storage container.
  • the reaction space of the patch PA is the surface of the patch PA, it can be easily observed through the surface of the patch PA.
  • the patch (PA) may be designed to be modified in a form that is easy to observe.
  • the liquid substance SB stored in the patch PA may be modified or react with other kinds of substances.
  • the liquid substance SB stored in the patch PA may have a composition changed over time.
  • the reaction may be a chemical reaction in which the chemical formula is changed, or may mean a physical state change or a biological reaction.
  • the liquid material SB stored in the patch PA may be a material of a single component or a mixture including a plurality of components.
  • the patch PA may capture, absorb, release, and / or store fluid material as described above.
  • the patch PA may implement various embodiments of the patch PA that perform a function of providing a path of movement of a material. However, some embodiments will be described for more specific understanding.
  • the patch PA may be implemented to perform 2.2.4.1 (ie, table of contents for delivery) and 2.2.4.2 (ie, table of contents for absorption) among the functions of the patch PA described above.
  • the absorption function and the delivery function may be provided together, may be provided sequentially.
  • the patch PA may perform the absorption and delivery functions together to provide a path of movement of the material.
  • Providing a path of movement of the foreign material by the patch PA may be performed by absorbing the foreign material and releasing the foreign material.
  • the patch PA may contact the external material to absorb the external material and contact the external area to transfer the external material to the external area.
  • the patch PA captures the foreign material and delivers the external material to the absorption and delivery process similar to the above-described absorption and delivery.
  • the foreign substance absorbed and delivered to the patch PA may be a liquid phase or a solid phase.
  • the patch PA may allow some materials to be transferred from the external material to the other external material.
  • the patch PA and the foreign material and other foreign material may be in contact at the same time.
  • the patch PA and the foreign material and other foreign materials may contact the patch PA at different times.
  • the patch PA, the external material, and another external material may be contacted at different time points.
  • the patch PA and the external material are contacted first, and after the external material and the patch PA are separated, the patch PA and the other external material are contacted.
  • the material may be contacted.
  • the patch PA may temporarily store a material captured from the external material.
  • the patch PA may additionally provide a delay in time while providing a path of movement of the material.
  • the patch PA may perform a function of appropriately adjusting the amount and rate of delivery of the substance to other foreign substances.
  • such a series of processes may be performed in one direction based on the patch (PA).
  • absorption of the material may be made through one surface of the patch PA, and an environment may be provided in the internal space of the patch PA, and the material may be released through the other surface facing the one side. Can be.
  • the patch PA may absorb and release the material among the functions of the patch PA and provide a reaction space of the material. At this time, the absorption, release and provision of the reaction space of the material may be performed simultaneously or sequentially.
  • the patch PA may provide a reaction space to the absorbed foreign material for at least some time in performing the process of absorbing and releasing the foreign material.
  • the patch PA may provide a specific environment for the liquid material SB captured in the patch PA including the absorbed external material for at least some time.
  • the liquid substance SB trapped in the patch PA and the external substance trapped in the patch PA may react inside the patch PA.
  • the foreign material absorbed by the patch PA may be affected by the environment provided by the patch PA.
  • the material released from the patch PA may include at least a part of the material produced through the reaction.
  • the external material may be released by changing the composition, properties, etc. from the patch (PA).
  • the absorbed material may be released from the patch PA. It can be understood that the foreign material is absorbed in the patch PA and released from the patch PA passes through the patch PA.
  • the external material passing through the patch PA may lose its identity due to the reaction inside the patch PA or the influence of the environment provided by the patch PA.
  • Absorption of the external material, reaction of the material, and delivery of the material may be performed in one direction.
  • absorption of the material may be performed at one location of the patch PA, provision of the environment at another location, and release of the material at another location.
  • the patch PA may provide a path of movement of the material between the plate PL1 coated with the seventh material SB7 and the plate PL2 coated with the eighth material SB8. have.
  • the patch PA may be attached to the plates PL1 and PL2.
  • the seventh material SB7 may be moved through the patch PA to be combined with the eighth material SB8 by contacting them.
  • the seventh material SB7 and the eighth material SB8 are connected to the patch PA in the water film WF formed by contacting the patches PA with the plates PL1 and PL2. You can.
  • 29 and 30 illustrate an embodiment of a patch PA according to the present application, which provides a path of movement of material between two patches.
  • the patch PA6 providing the movement path may be in contact with the patch PA5 storing the movement target material and the patch PA7 receiving the movement target material.
  • the patch PA6 providing the movement path contacts the patch PA5 for storing the substance to be moved and the patch PA7 for receiving the substance to be moved. ) Can be moved.
  • the movement of material between each patch can be achieved through the water film WF formed near the contact area between the patches.
  • 31 and 32 illustrate an embodiment of a patch according to the present application, which provides a path of movement of material between two patches.
  • the patch PA9 providing the movement path may be in contact with the patch PA8 storing the ninth material SB9 and the patch PA10 receiving the material.
  • the patch PA9 providing the movement path may absorb the ninth material SB9 by contacting the patch PA8 storing the ninth material SB9.
  • the absorbed ninth material SB9 may react with the tenth material SB10 stored in the patch PA9 providing the movement path to form the eleventh material.
  • the eleventh material SB11 may be transferred from the patch PA9 providing the movement path to the patch PA10 receiving the material.
  • the movement of the material between the patches PA may be performed through the water film WF formed near the contact area between the patches PA.
  • the patch PA may be used alone, or a plurality of patches PA may be used together.
  • that the plurality of patches PA may be used together includes not only the case where they are used simultaneously but also the case where they are used sequentially.
  • each patch PA may perform a different function.
  • Each patch PA of the plurality of patches PA may store the same material, but may store different materials.
  • each patch PA is not in contact with each other so that the movement of the material between the patches PA may not occur, or the mutual exchange of materials stored in each patch PA may occur. It is also possible to perform the desired function in the possible state.
  • the plurality of patches PA used together may be manufactured in a similar shape or the same standard, but may be used together in the case of a plurality of patches PA having different shapes.
  • each patch PA constituting the plurality of patches PA may have different densities of the net structure NS, or different components forming the net structure NS.
  • the plurality of patches PA may contact one target area TA.
  • the plurality of patches PA may contact one target area TA to perform a desired function.
  • the plurality of patches PA may contact different target areas TA when the plurality of target areas TA is plural. When the plurality of target areas TA is present, the plurality of patches PA may contact the target areas TA corresponding to the plurality of patches PA to perform a desired function.
  • the plurality of patches PA may be in contact with a material applied to the target area TA.
  • the material applied to the target area TA may be fixed or have fluidity.
  • the desired function may be a delivery or absorption function of a substance.
  • each patch PA does not necessarily deliver the same material or absorb the same material, and each patch PA delivers a different material to the target area TA, or is located in the target area TA. It can absorb different components from the material.
  • the desired function may be different for each patch PA constituting the plurality of patches PA.
  • one patch PA may perform a function of transferring a material to the target area TA
  • the other patch PA may perform a function of absorbing a material from the target area TA.
  • the plurality of patches PA may include different materials, and the different materials may be delivered to one target area TA to induce a desired reaction.
  • the plurality of components may be stored in the patch PA and delivered to the target area TA.
  • the use of such a plurality of patches (PA) may be particularly useful when the materials required for the reaction are mixed, such as stored in a single patch (PA), if the properties of the materials required for the desired reaction are lost or altered. have.
  • the material of the different components when the plurality of patches (PA) comprises a material of different components and the material of the different components have different specific binding relationship, the material of the different components to the target region ( TA).
  • the plurality of patches PA may be used to detect a plurality of specific bindings from a material applied to the target area TA by transferring materials of the different components.
  • the plurality of patches PA may include materials of the same component, and each patch PA may have a different concentration with respect to the materials of the same component.
  • the plurality of patches PA including the materials of the same component may contact the target area TA and may be used to determine the influence of the concentration of the materials included in the plurality of patches PA.
  • the configuration of the plurality of patches PA to be used can be used differently each time. That is, the plurality of patches PA can be manufactured and used in the form of a cartridge. At this time, the shape of each patch PA used can also be suitably standardized and manufactured.
  • the plurality of patches PA in the form of cartridge may be suitable when a patch PA for storing a plurality of types of substances is prepared, and if desired, the selected patch PA is used.
  • a combination of specific reactions to be detected may be configured and performed each time the detection is performed. There will be.
  • FIG. 33 illustrates an embodiment of a patch PA according to the present application, in which a plurality of patches PA are used together.
  • the plurality of patches PA according to the exemplary embodiment of the present application may be simultaneously in contact with the target area TA positioned on the plate PL.
  • Each patch PA constituting the plurality of patches PA may have a standardized form.
  • the plurality of patches PA may include a first patch and a second patch, and a material stored in the first patch may be different from a material stored in the second patch.
  • the plate PL includes a plurality of target areas TA.
  • the plurality of patches PA according to the exemplary embodiment of the present application may be simultaneously in contact with the plurality of target areas TA positioned on the plate PL.
  • the plurality of patches PA includes a first patch PA and a second patch PA, and the plurality of target areas TA includes a first target area and a second target area.
  • the patch may contact the first target area and the second patch may contact the second target area.
  • the plurality of patches PA may perform a plurality of functions. As described above, each patch PA may perform a plurality of functions at the same time, and each patch PA may perform a different function at the same time. However, the present invention is not limited thereto, and each function may be performed in combination in a plurality of patches PA.
  • each patch PA may perform both storage and release of the material.
  • each patch PA may store a different material and release each stored material in the target area TA. In this case, each stored material can be released simultaneously or sequentially.
  • each patch PA may be performed by dividing the storage and release of the material. In this case, only some of the patches PA may be in contact with the target area TA, and may release the material into the target area TA.
  • each patch PA can simultaneously perform storage, release and absorption of the material.
  • each of the patches PA may be performed by dividing the storage, release and absorption of the material.
  • the present invention is not limited thereto, and each function may be performed in combination in a plurality of patches PA.
  • At least some of the plurality of patches PA may store a material and release the stored material to the target area TA. In this case, at least some other of the plurality of patches PA may absorb the material from the target area TA. Some of the plurality of patches PA may emit a material specifically binding to a material positioned in the target area TA. In this case, detection of specific binding may be performed by absorbing a material that does not form the specific binding among the materials located in the target region TA using another patch PA.
  • each patch PA may simultaneously perform storage, release and provision of the environment at the same time.
  • each of the patches PA may perform a separate storage, release and provision of the environment.
  • the present invention is not limited thereto, and each function may be performed in combination in a plurality of patches PA.
  • one patch PA among the plurality of patches PA may release the stored material to the target area TA.
  • another patch PA may provide an environment to the target area TA.
  • the providing of the environment may be implemented in a form of transferring the environmental conditions of the material stored in the other patch PA to the target area TA.
  • the reactant may be provided to the target area TA by one patch PA, and the other patch PA may contact the target area TA to provide a buffer environment.
  • the plurality of patches PA may be in contact with each other.
  • the at least one patch PA may store the material and release the stored material as another patch PA providing the environment.
  • the patch PA providing the environment is in contact with at least one patch PA that releases the material and is not in contact with each other, and can absorb the material from each patch PA.
  • the patch of the present application can be used for immunodiagnosis.
  • Immunodiagnosis refers to diagnosis based on test results obtained by immunological techniques.
  • an enzyme linked immunosorbent assay hereinafter referred to as ELISA
  • ELISA enzyme linked immunosorbent assay
  • the base material (BS) and the additive material (AS) described above may be appropriately changed depending on the application.
  • Immune diagnosis can be distinguished according to various criteria.
  • an antigen is immobilized on a plate (PL), and a direct method (or direct ELISA) that detects the amount of antigen by directly binding an enzyme to an antibody reacting with the antigen
  • a direct method or direct ELISA
  • Indirect method or indirect ELISA
  • an antibody against the antigen Sandwich method or sandwich ELISA
  • sandwich ELISA which is immobilized on the plate (PL), binds the antigen, and detects the antigen using direct or indirect methods, and 4) using the two antigens competing for the same binding site of the antibody. It can be considered by dividing into a competitive quantitative method (or competitive ELISA) to measure concentrations.
  • Immune diagnosis can also be classified according to the subject sample.
  • the sample When the sample is a body fluid (bodily fluid), it may be classified into immunochemistry, immunocytochemistry if the sample is a cell, and immunohistochemistry if the sample is a tissue.
  • Immunodiagnosis for body fluids can be used for diagnosis through detection of target proteins such as floating antigens.
  • Immunodiagnostics for cells can be used to detect target proteins, such as antigens present on or inside the cells.
  • Immunodiagnostics for tissues can be performed in a manner that detects target proteins present on or within the cell or determines the distribution of target proteins in tissues.
  • Immune diagnosis can also be distinguished by detection method.
  • colorimetric a method of observing color development by the reaction product of an enzyme and a substrate
  • chemiluminescence a method of detecting light emitted by a chemical reaction
  • chemifluorescence chemifluorescence
  • ELISA is an example of an immunodiagnosis, and is a diagnostic method for detecting a substance, especially an antigen.
  • ELISA means the method of attaching an enzyme to an antibody or an antigen, measuring the activity of an enzyme, and quantitatively measuring the intensity and the quantity of an antigen-antibody reaction.
  • an antibody or antigen can be adsorbed on a solid phase, and free antigen or antibody which is not bound can be removed by washing, so that a desired result can be easily detected.
  • the immunodiagnosis is intended to mean the immunodiagnosis by the ELISA mode.
  • Specimens used in immunodiagnosis can be broadly divided into body fluids, cells, and tissues, each of which may require appropriate treatment in order to be used for diagnosis.
  • Fluid samples used for immunodiagnosis may include blood, urine, saliva, and the like.
  • blood may be used as whole blood, but may be separated for each component such as serum, plasma, and blood cells and used for detection.
  • cell sample used for the immunodiagnosis whole blood, cultured cells, cell suspensions and the like can be used.
  • the tissue is considered to be a sample.
  • the preparation of the sample used for immunodiagnosis is demonstrated.
  • the mode of preparation of the samples used for diagnosis may differ depending on how the diagnosis is to be performed.
  • a case of performing diagnosis on a sample located on the plate PL using the patch PA of the present application will be described.
  • the plate PL may mean a solid plate such as a plate made of general slide glass, polystyrene, polypropyrene, or the like.
  • the plate PL may have a different shape or transparency depending on a detection method.
  • the plate PL may be a white plate or a black plate.
  • the plate PL may have a flat bottom and be transparent.
  • the plate PL may include a reaction region in contact with the patch PA or in which a desired reaction may occur.
  • the body fluid When performing an immunological diagnosis on the body fluid, the body fluid may be used for diagnosis while being fixed to the plate PL. In other words, the body fluid may be used in a state where it is plated and fixed to the plate PL. Alternatively, the body fluid may be plated and used on the plate PL. For example, it can be used in such a way that the body fluid is smeared on the plate PL to which the antibody is immobilized.
  • the suspension or blood of the cells may be plated on the plate PL and dried to perform the diagnosis.
  • the suspension or blood of the cells may be plated on the plate PL for use.
  • the diagnosis may be performed by plating a suspension or blood of the cells on the plate PL to which the antibody is immobilized.
  • the cells that have undergone the cell lysis (lysis) treatment, or the cells that have not undergone the cell lysis treatment can be diagnosed.
  • tissues prepared from slices or flakes may be placed on the plate PL and the diagnosis may be performed.
  • the sections of tissue may be paraffin filled tissue sections or frozen tissue sections.
  • the aforementioned patch may be used.
  • the patch PA may store antigen and deliver the antigen to the plate PL.
  • the patch PA may store a biological sample, that is, a specimen, containing an antigen and deliver the sample to the plate PL.
  • the patch PA stores the antibody AB and transfers the antibody to the plate PL.
  • the antibody (AB) stored in the patch PA may be a primary antibody (AB) or a secondary antibody (AB).
  • the patch PA may store the primary antibody AB or the secondary antibody AB.
  • the patch PA may store the primary antibody AB and the secondary antibody AB together and deliver them to the plate PL.
  • the antibody (AB) may be stored in the patch in a state of being attached to separate particles.
  • the patch PA may store and transfer the substrate SU that performs the reaction catalyzed by the enzyme to the plate PL.
  • the substrate (SU) used may vary depending on the enzyme used and the detection method.
  • the substrate (SU) may be 3-ethylbenzothiazoline-6-sulphonic acid (ABTS), TMB (3,3 ', 5,5'-Tetramethylbenzidine), or the like.
  • the patch PA may store the washing solution and absorb the residue from the plate PL.
  • the patch PA stores the washing solution, contacts the patch PA, and separates the patch PA to absorb and remove impurities of the plate PL and antibodies AB that are not specifically bound.
  • the washing solution used may be TBS or PBS to which tween-20 is added.
  • the patch PA may store a buffer solution and provide an environment to the plate PL.
  • the buffer solution may play a role of smoothly performing each step of the immune diagnosis.
  • the buffer solution used in each step may contain other components.
  • a hydrogen peroxide buffer may be used when detecting chemiluminescence.
  • the patch PA may store a stop solution for stopping the reaction of the substrate SU with the enzyme. That is, the reaction stop patch PA may be prepared, and the stop solution may be transferred to the plate PL using the reaction stop patch PA to stop the reaction of the substrate SU and the enzyme at a suitable time. have.
  • the immunodiagnostic method in the present invention is not limited to the examples described below, and may be applied throughout the immunodiagnostic method performed using the patch (PA), as there may be many modified detection methods. Can be.
  • the patch (PA) and plate (PL) of the present application can be used to perform an immunological diagnosis according to the direct method.
  • the sample (or antigen) is immobilized on the plate (PL), specifically binds to the antigen to be detected and the identification label
  • this is carried out by applying an antibody (AB) to which an enzyme is attached, and removing the antibody (AB) that does not form a specific bond, thereby detecting the reaction of the substrate (SU) catalyzed by the enzyme.
  • the patch (PA) according to the present application may be used to apply the antibody (AB) and to remove the antibody (AB) that does not form the specific bond.
  • the patch PA and the plate PL of the present application may be used to perform an immunological diagnosis according to an indirect method.
  • Performing an immunodiagnosis according to the indirect method using the patch PA and the plate PL may be performed by immobilizing a sample (or antigen) on the plate PL, and binding the antibody specifically to the antigen to be detected ( AB) (i.e., primary antibody (AB)), remove the primary antibody (AB) that did not form the specific binding, bind specifically to the primary antibody (AB), and identify the label
  • AB specifically to the antigen to be detected
  • An antibody (AB) having an enzyme attached thereto i.e., a secondary antibody
  • the patch (PA) may be used in the step of applying the antibody (AB) and removing the antibody (AB) that did not form the specific binding.
  • the patch PA and the plate PL of the present application may be used to perform an immunological diagnosis according to the sandwich method.
  • the antibody (AB) is fixed to the plate (PL), the sample (or antigen) to the plate (PL) It can be carried out by applying, applying an antibody (AB) that specifically binds to the antigen to be detected, and removing the antigen to the antibody (AB) that does not form a specific binding.
  • applying the antibody (AB) that specifically binds to the antigen may be performed by the direct method or indirect method described above.
  • the application of the antibody (AB) that specifically binds to the antigen may be performed by applying the antibody (AB) that specifically binds to the antigen and to which the enzyme is attached.
  • applying the antibody (AB) that specifically binds to the antigen may include applying a primary antibody (AB) that specifically binds to the antigen and reacting specifically with the primary antibody (AB). It may be to apply an antibody (AB) to which an enzyme is attached as an identification label. Patches according to the present application (PA) can be used to apply the antibody (AB).
  • Detection of the diagnosis result of the immunodiagnostic using the patch PA and the plate PL of the present application may be performed by various detection methods.
  • the detection method may be selectively used depending on the product produced by the reaction of the enzyme with the substrate (SU).
  • the antibody (AB) in the direct method or the secondary antibody (AB) in the indirect method may have an enzyme attached as a label, and the enzyme attached to the antibody (AB) may react with the chemical reaction of the substrate (SU).
  • the product may be catalyzed to generate a product, and the detection method may be determined differently according to the product generated at this time.
  • the color development When color development is detected by the resulting product, the color development can be measured to quantitatively determine specific binding.
  • the color measurement may be performed by detecting light emitted from a light source and passing through the plate PL. In other words, measuring color development may be performed in a manner of measuring absorbance.
  • a spectrophotometer When measuring the color, a spectrophotometer may be used.
  • the plate PL may be transparent and flat.
  • the plate PL When luminescence is detected by the generated product, specific binding can be measured quantitatively by measuring it. Measuring the light emission may be performed by detecting light emitted from a solution on or at the bottom of the plate PL. When measuring the luminescence, a luminometer can be used. In the case of measuring the light emission, preferably, the plate PL may be opaque black or opaque white.
  • fluorescence When fluorescence is detected by the generated product, specific binding can be quantitatively determined by measuring it.
  • the fluorescence may be measured by injecting light into the plate PL and measuring fluorescence emitted from the plate PL.
  • a fluorometer with a filter When measuring the fluorescence, a fluorometer with a filter may be used.
  • the plate PL In the case of measuring the fluorescence, preferably, the plate PL may be opaque black or opaque white.
  • a color image, a light emission image or a fluorescent image can be taken.
  • the images may be photographed in parts and may be collected as one. From the images obtained by photographing, a distribution position of a target antigen / antibody (AB), a shape of a cell, a distribution of a target protein in a tissue, and the like may be obtained. In addition, by analyzing the images obtained by the photographing, it is also possible to obtain a location of a target protein, a target antigen / antibody (AB) and the like and partial images of the target.
  • AB target antigen / antibody
  • Detection of the diagnosis result of the immunodiagnosis may be performed by an electrochemical method.
  • a change in electrochemical properties occurring on the plate PL may be measured by the antibody AB specifically bound to a sample immobilized on the plate PL.
  • the patch PA may measure a change in electrochemical properties of the patch PA generated by delivering the antibody AB to the plate PL.
  • Positioning the sample to be diagnosed includes a method of fixing the sample to the plate PL, a method of smearing the sample to the plate PL, or a plate to fix the sample to the plate PL. It may be performed by any one of the methods.
  • the immunodiagnostic method in addition to positioning the sample (S200) and delivering the antibody (AB) to the reaction region (S300), a chemical reaction catalyzed by an enzyme attached to the antibody (AB) Providing the substrate (SU) to the reaction zone by using a patch (PA) for storing the substrate (SU) to produce a product through (S36) (FIG. 36)
  • the immunodiagnostic method in addition to positioning the sample (S200) and delivering the antibody (AB) to the reaction region (S300), in order to diagnose a target disease, the antibody (AB) and the target
  • the method may further include detecting a specific reaction of the protein (TP) (S500) (FIG. 37).
  • detecting the specific reaction may be measuring a change in electrical characteristics of the patch (PA) generated by the specific reaction.
  • the detection of the specific reaction may be performed by measuring a fluorescence generated by a chemical reaction catalyzed by an enzyme attached to an antibody (AB) that specifically binds to the target protein (TP), or by the chemical reaction. It may be based on at least one of measurement of the emitted light emission or measurement of the colorimetric generated by the chemical reaction.
  • the delivering of the antibody (AB) to the reaction region (S300) includes contacting the patch (PA) with the reaction region so that the antibody (AB) can move to the reaction region (S310) and the patch. Separating (PA) from the reaction region (S330), and when the patch (PA) is separated from the reaction region, the antibody that does not specifically react with the target protein (TP) of the antibody (AB) (AB) can be removed from the reaction zone (FIG. 38).
  • the delivered antibody (AB) using a washing patch (PA) may further include absorbing the antibody (AB) that does not specifically react with the target protein (TP) from the reaction region (S600) (FIG. 39).
  • delivering the antibody (AB) to the reaction region (S300) may include a first patch (PA) storing a first antibody (AB) that specifically reacts with the target protein (TP). Delivering the first antibody (AB) to the reaction region using the step (S320) and storing the second antibody (AB) that specifically reacts with the first antibody (AB) (PA). It may include a step (S340) for delivering the second antibody (AB) to the reaction region using the ().
  • the bottom antibody (BAB) which is an antibody (AB) that specifically reacts with the target protein (TP) is placed before the step of placing a sample to be diagnosed in the reaction region (S200).
  • the method may further include providing the plate PL fixed to an area (S100), and placing the sample to be diagnosed in the reaction area may include a diagnosis object in a reaction area in which the bottom antibody (BAB) is fixed. It may be to place the sample to be (FIG. 41).
  • the immunodiagnostic method may further include placing the sample (S200), delivering the antibody (AB) to the reaction region (S300), and providing a substrate (SU) to the reaction region (S400).
  • the method may further include the step S700 of stopping the chemical reaction of the substrate SU (FIG. 42).
  • FIG. 43 is a flowchart illustrating an immunodiagnostic method by indirect ELISA as an example of an immunodiagnostic method according to the present application.
  • a step (S200) of placing a sample (SA) in a reaction region, delivering a first antibody (AB) to the reaction region (S320), and a reaction Delivering a second antibody (AB) to a region (S340), providing a substrate (SU) to a reaction region (S400), and detecting a specific reaction of the antibody (AB) and a target protein (TP) ( S500) may be included.
  • Immune diagnosis according to an embodiment of the present application using the plate (PL) and the patch (PA), can be performed by indirect ELISA.
  • Fixing the sample SA to the plate PL may fix the sample SA to be diagnosed to the plate PL.
  • the fixing of the sample SA to the plate PL may be to dry and fix the bodily fluid sample SA to be diagnosed on the plate PL.
  • Fixing the specimen SA to be diagnosed on the plate PL may be to fix the tissue section.
  • the first patch (PA) in which the primary antibody (AB) is stored in contact with the plate (PL) plate (PL) may be to deliver the primary antibody (AB).
  • the first patch PA may be in contact with the plate PL, and the first patch PA may be in contact with the specimen SA fixed to the plate PL.
  • the first patch PA may be in contact with the plate PL, and the first patch PA may be in contact with an area of the plate PL on which the specimen SA is fixed.
  • the primary antibody (AB) may be determined differently whenever it is desired to detect another antigen.
  • the primary antibody (AB) may be an antibody (AB) that specifically binds to the antigen to be detected.
  • Delivering the primary antibody (AB) using the first patch (PA), the step of contacting the first patch (PA) to the plate (PL) and the first patch (PA) to the plate (PL) may comprise the step of separating. Contacting the first patch PA with the plate PL and separating from the plate PL may allow the primary antibody AB to be selectively delivered. In other words, when the antigen to which the primary antibody (AB) specifically binds is immobilized on the plate (PL), the primary antibody (AB) may be selectively delivered from the first patch (PA). have.
  • the primary antibody (AB) (hereinafter, referred to as a surplus primary antibody) that has moved to the plate (PL) without forming a specific binding is formed as the first patch (PA) is separated from the plate (PL). It may be absorbed into the first patch PA and removed from the plate PL.
  • the water film formed by contacting a patch with a sample may refer to a thin liquid film near a contact area. At this time, the film formed does not necessarily have a flat shape.
  • the present invention is not limited thereto, and in the present application, the water film may be understood as referring to a region in which a patch is in contact with a sample and the like, and a region in which the patch and the sample are connected and the material has fluidity.
  • the washing process that was necessary for performing the conventional ELISA can be omitted.
  • the primary antibody (AB) that did not form a specific binding using the washing solution from the plate (PL) The process of removal may be omitted.
  • the patch PA stores the primary antibody AB1 and stores the primary antibody AB1 as a sample SA or a reaction region in which the sample SA is located.
  • Primary antibody (AB1) can be delivered.
  • the patch PA delivers the primary antibody AB1 to the plate PL through the water film WF formed near the contact area by contacting the patch PA with the plate PL.
  • the primary antibody AB may be formed by being able to move to the plate PL or the reaction region of the plate PL.
  • the delivery of the primary antibody (AB1) to the plate (PL) is specific for the target antibody (AB1) and the target protein (TP) contained in the sample (SA), particularly the sample (SA) It can be combined.
  • the secondary antibody (AB) may be an antibody (AB) that specifically binds to the primary antibody (AB).
  • the secondary antibody (AB) may be determined differently depending on the primary antibody (AB) used.
  • the secondary antibody (AB) may be an antibody attached to the enzyme (AB).
  • the enzyme attached to the antibody may be HRP or AP.
  • Delivering the secondary antibody (AB) using the second patch (PA), the step of contacting the second patch (PA) to the plate (PL) and the second patch (PA) to the plate (PL) may comprise the step of separating.
  • the secondary antibody AB may be selectively delivered to the plate PL by contacting the second patch PA to the plate PL and separating it from the plate PL.
  • the primary antibody (AB) that specifically binds to AB) can optionally be delivered to the plate (PL).
  • the secondary antibody (AB) (hereinafter, referred to as a surplus secondary antibody) that has moved to the plate (PL) without forming a specific binding is formed as the second patch (PA) is separated into the plate (PL). Adsorbed on the second patch PA may be removed from the plate PL. Accordingly, the process of removing the antibody (AB) that does not form a specific bond by using the washing solution from the plate (PL) may be omitted.
  • the excess secondary antibody (AB) is absorbed into the second patch (PA), the aqueous membrane formed by the excess secondary antibody (AB) in contact with the plate (PL) ( It may be performed by dissolving in WF and moving the water film WF with the first patch PA when the second patch PA is separated from the plate PL.
  • the excess secondary antibody (AB) is the plate (PL) only by separation of the second patch (PA) Can be removed from. Accordingly, the process of removing the secondary antibody (AB) that does not form a specific binding from the plate (PL) by using the washing solution, which was essentially required in performing the ELISA, may be omitted.
  • the patch PA stores the secondary antibody AB3 and moves to the sample SA positioned on the plate PL or the reaction area in which the sample SA is located.
  • Primary antibody (AB4) can be delivered.
  • the patch PA delivers the secondary antibody AB4 to the plate PL through the water film WF formed near the contact area by contacting the patch PA with the plate PL.
  • the secondary antibody AB2 may be formed by being able to move to the plate PL or the reaction region of the plate PL.
  • the delivery of the secondary antibody (AB2) to the plate (PL) is a binding to the target antibody (TP) contained in the secondary antibody (AB2) and the sample (SA), in particular the sample (SA) Specific binding with the primary antibody (AB1) can be achieved.
  • the immunodiagnostic method according to the present embodiment may further include providing a substrate (SU) using a patch (PA).
  • Providing the substrate SU using the patch PA on the plate PL may contact the plate PL with the third patch PA having the substrate SU stored therein. It may be to provide a substrate (SU).
  • the substrate SU may be ABTS or TMB.
  • the substrate (SU) may be catalyzed by the enzyme to generate a product (PD), and the product (PD) may serve as a label of the specific binding to be detected.
  • the providing of the substrate SU using the above-described third patch PA may include contacting the third patch PA to the plate PL and the third patch PA. It may include the step of separating from the plate (PL).
  • the contact time between the third patch PA and the plate PL may be adjusted by contacting the third patch PA to the plate PL and separating the plate PL from the plate PL.
  • the third patch PA may be separated from the plate PL at an appropriate time point.
  • the immunodiagnosis may be performed by detecting a product (PD) generated by a chemical reaction of the substrate (SU) catalyzed by the enzyme, wherein if the reaction is not stopped at an appropriate time point, It may be difficult to quantitatively measure the product (PD) due to the excessive production of the product (PD).
  • the reaction may be stopped at an appropriate time point, so that the measurement error due to the above-described excessive reaction may be eliminated.
  • the specific binding can be detected by measuring the product (PD) up to the point at which the substrate (SU) patch (PA) is removed. Can be.
  • the patch PA stores the substrate SU
  • the substrate SA may be a sample SA positioned on the plate PL or a reaction area in which the sample SA is located.
  • Providing the substrate SU to the plate PL by the patch PA is such that the patch PA contacts the plate PL to form the substrate SU through the water film WF formed near the contact area.
  • the SU may be formed by being movable to the plate PL or the reaction region of the plate PL.
  • the substrate SU may be produced or converted into the product PD by a chemical reaction catalyzed by an enzyme attached to the secondary antibody AB located on the plate PL.
  • the immunodiagnostic method may further include absorbing the residue by using the washing patch PA.
  • Absorbing the residue using the washing patch PA may contact the washing patch PA with the plate PL to absorb the residue.
  • Absorption of the residue using the washing patch PA may include a first process in which the washing patch PA is not specifically bound to at least a portion of the fixed sample SA by contacting the plate PL. It may be to absorb the antibody (AB).
  • Absorption of the residue using the washing patch PA may include a second process in which the washing patch PA is not specifically bound to at least a portion of the first antibody AB by contacting the plate PL. It may be to absorb the antibody (AB).
  • Absorption of the residue using the washing patch PA may be performed after the delivery of the primary antibody AB to the plate PL using the first patch PA and to the plate PL.
  • the second patch (PA) may be used prior to the delivery of the secondary antibody (AB).
  • the step of absorbing the residue using the washing patch (PA), after the step of delivering the secondary antibody (AB) using the second patch (PA) to the plate (PL) and the plate (PL) ) May be performed prior to providing the substrate SU using the patch PA.
  • Absorption of the residue by using the washing patch PA may replace the washing using the washing solution in the conventional immunodiagnostic method.
  • the conventional washing method is mainly performed by rinsing the washing solution by pouring it onto the plate PL in order to remove a substance which is located on the plate PL but does not form a specific bond, so that the consumption of the solution is excessive.
  • the consumption of the washing solution is significantly reduced compared to the conventional method has the effect of improving the economic efficiency.
  • the patch PA may absorb excess material from the plate PL.
  • the surplus substance may be an antibody (AB4) that is not specifically bound to the target protein (TP).
  • the antibody (AB3) to which the target protein (TP) specifically binds may not be absorbed by the patch.
  • the patch PA may be a washing patch PA for storing a washing solution.
  • the immunodiagnostic method according to the present embodiment may include deterge of substances that interfere with the detection of the specific binding.
  • a cleaning patch PA may be used to easily remove substances that interfere with the detection of the specific binding from the plate PL.
  • the immunodiagnostic method according to the present embodiment may further include providing a predetermined environment to the plate PL.
  • Providing the predetermined environment may be performed using a buffer patch PA.
  • Providing the predetermined environment may be to provide an environment using a buffer patch (PA) for storing a buffer solution to facilitate each step of the immune diagnosis.
  • a buffer patch (PA) in which a peroxide buffer is stored may be used in detecting chemiluminescence.
  • the immunodiagnostic method according to the present embodiment may include stopping the reaction in the plate PL. Stopping the reaction may be performed using a stop patch (PA). Stopping the reaction may be to stop the chemical reaction of the substrate (SU) catalyzed by the enzyme, that is, the product (PD) production reaction. Stopping the reaction may be to terminate the reaction.
  • PA stop patch
  • the immunodiagnostic method may further include detecting a specific response, which may be to detect a product (PD) production response of the substrate (SU). Detecting the production reaction may be to detect an antigen specifically bound to the primary antibody (AB). Detecting the antigen specifically bound by the primary antibody (AB) is generated by the reaction of the substrate (SU) catalyzed by an enzyme attached to the secondary antibody (AB) bound to the primary antibody (AB). It may be to detect the product (PD). In this case, detecting the product (PD) may be implemented by measuring color development due to the reaction, light emission due to the reaction, or measuring fluorescence due to the reaction.
  • Detecting the production reaction may be performed by contacting the patch PA, in which the substrate SU is stored, with the plate PL, and detecting the chemical reaction of the substrate SU in real time. Detecting the production reaction may be performed by contacting the patch PA, in which the substrate SU is stored, with the plate PL, and detecting the reaction result after a predetermined time. In this case, after a certain point of time, the patch PA in which the substrate SU is stored may be separated and a reaction result may be detected.
  • the patch PA in which the suspension solution is stored is contacted with the plate PL, and the reaction result is detected in a state in which the patch PA in which the suspension solution is stored is in contact with the plate PL.
  • the reaction result may be detected in a state in which the patch PA in which the suspension solution is stored is separated from the plate PL.
  • the substrate (SU) or to deliver the primary antibody (AB) or the secondary antibody (AB) it is always that all must be performed using the patch (PA) no.
  • Some of the steps of providing the substrate (SU) or delivering the primary antibody (AB) or the secondary antibody (AB) are in the liquid to solution state of the substrate (SU), primary antibody (AB) or secondary
  • the antibody (AB) may be replaced by a form for delivering the plate (PL).
  • each patch PA is described as having several components, and each component can be understood as the base material (BS) or additive material (AS) described above.
  • BS base material
  • AS additive material
  • components described as being able to be stored in each patch PA are not all components stored in each patch PA, and each patch PA may be stored together with additional components not specified.
  • Immune diagnostics of the present application can be performed using a patch (PA) in which the primary antibody is stored.
  • the patch PA may store the primary antibody and deliver the primary antibody AB to the plate PL.
  • the primary antibody (AB) may be the additive substance (AS) stored in the patch (PA).
  • the patch PA may store a solution containing the primary antibody AB.
  • the patch (PA) in which the primary antibody (AB) is stored together with the primary antibody (AB) or the primary antibody (AB) solution, easily binds the primary antibody (AB) to the antigen to be detected.
  • a separate base material (BS) or additive material (AS) may be stored.
  • the primary antibody (AB) may be an antibody (AB) that specifically binds to a target protein (TP).
  • the primary antibody (AB) may be an antibody (AB) that specifically binds to a target antigen to be detected.
  • the patch (PA) having the primary antibody (AB) stored therein may store the primary antibody (AB) that specifically binds to the antigen to be detected in the form of a solution.
  • the primary antibody (AB) may be uniformly distributed in the patch (PA).
  • the primary antibody (AB) may be stored and absorbed into the patch (PA) from a separate medium.
  • the primary antibody AB may be stored in the patch PA while attached to the microparticles.
  • the primary antibody (AB) when the primary antibody (AB) is stored in the patch (PA) and delivered to the plate (PL), the specific antibody fixed to the plate (PL) does not specifically bind to the The primary antibody (AB) can be absorbed back into the patch (PA). Accordingly, the washing process of the primary antibody (AB) may be omitted, in some cases it may be possible to reuse the patch (PA), and rapid and efficient diagnosis may be realized.
  • the antibody (AB) that specifically reacts with the target protein (TP) and provided in a net structure that forms the microcavities in which the antibody (AB) is stored and the target It may be an antibody (AB) storage patch (PA) comprising a net structure (NS) in contact with the reaction region in which the protein (TP) is located to deliver a portion of the stored antibody (AB) to the reaction region.
  • the antibody (AB) that specifically reacts with the target protein (TP) may be a primary antibody (AB) having specific binding to the target antigen.
  • Immune diagnosis of the present application can be performed using a patch (PA) in which the secondary antibody (AB) is stored.
  • the patch PA may store the secondary antibody AB and deliver the secondary antibody AB to the plate PL.
  • the secondary antibody (AB) may be an additive substance (AS) stored in the patch (PA).
  • the patch PA may store a solution containing the secondary antibody AB.
  • BS base material
  • AS additive material
  • the secondary antibody (AB) may be an antibody (AB) that specifically binds to the primary antibody (AB).
  • the specific binding of the primary antibody (AB) and the secondary antibody (AB) may be a species specific binding rather than an epitope specific binding.
  • the primary antibodies (AB) are of homologous origin. In other words, secondary antibodies (AB) with an identification label may be commonly used.
  • An enzyme may be attached to the secondary antibody (AB).
  • the enzyme attached to the secondary antibody (AB) may have the function of an identifier for the detection of the specific binding.
  • the enzyme is attached to the antibody (AB), and specifically binds to the antigen (AB) that specifically binds to the antigen (AB) or to the antibody (AB).
  • the function of a label or a reporter for detecting an antigen can be performed.
  • These enzymes can be used by binding the antibody (AB) to bind to the fragment crystallizable (FC) region of the molecule.
  • the enzyme is a basic phosphatase (AP) horseradish peroxidase (HRP, Horseradish Peroxidase) is mainly used.
  • AP basic phosphatase
  • HR horseradish peroxidase
  • the secondary antibody (AB) which does not specifically bind to some substances fixed to the plate (PL) is again the patch. As it can be absorbed into (PA), the performance of a rapid and efficient immune diagnosis can be realized.
  • the antibody (AB) that specifically reacts with the target protein (TP) is provided in a mesh structure forming the microcavities in which the antibody (AB) is stored, It may be an antibody (AB) storage patch (PA) comprising a net structure (NS) in contact with the reaction region where the target protein (TP) is located to deliver a portion of the stored antibody (AB) to the reaction region.
  • the antibody (AB) that specifically reacts with the target protein (TP) may be a secondary antibody (AB) having specific binding to the antibody (AB) having specific binding to the target antigen.
  • Immune diagnosis of the present application can be performed using a patch (PA) in which the substrate (SU) is stored.
  • the patch PA may store the substrate SU and provide the substrate SU to the plate PL.
  • the substrate SU may be an additive material AS stored in the patch PA.
  • the patch PA may store a solution including the substrate SU.
  • the patch PA in which the substrate SU is stored may further store the base material BS or the additive material AS that assists the product PD production reaction of the substrate SU.
  • the enzyme attached to the secondary antibody (AB) may catalyze the chemical reaction of the substrate (SU).
  • the specific binding described above can be detected from the product (PD) produced by the reaction catalyzed by the enzyme.
  • the substrate SU may be catalyzed by the enzyme to generate a product PD, and the product PD may be detected to detect the specific binding.
  • the substrate (SU) may be 3-ethylbenzothiazoline-6-sulphonic acid (ABTS), TMB (3,3 ', 5,5'-Tetramethylbenzidine), or the like.
  • the substrate (SU) used may be determined differently depending on the enzyme used and the detection method. For example, when the enzyme used is AP, color development can be detected using para-Nitrophenylphosphate (pNPP) as a substrate (SU). As another example, when the enzyme used is HRP, color development can be detected using TMB or the like as a substrate (SU).
  • the conventional ELISA in which the substrate SU solution is poured into the reaction solution of the plate PL to detect the reaction
  • the amount of substrate (SU) solution consumed is significantly smaller. Therefore, the diagnosis can be performed economically.
  • the substrate (PA) patch (PA) can be separated at an appropriate time to prevent overreaction, more accurate detection results can be obtained.
  • the immunodiagnostic method according to the present embodiment may be performed using a washing patch (PA) that absorbs the residue.
  • the immunodiagnostic method according to the present embodiment may absorb the residue by contacting and separating the washing patch PA from the plate PL.
  • the residue is obtained when the above-described primary antibody (AB) patch (PA), secondary antibody (AB) patch (PA) or substrate (SU) patch (PA) is contacted with the plate (PL) and then separated.
  • AB primary antibody
  • PA secondary antibody
  • SU substrate
  • the washing patch PA may store a washing solution.
  • the washing solution may include TBS and PBS to which tween-20 is partially added.
  • the washing solution may be provided as a solution in which the residue may be dissolved, depending on the residue to be absorbed.
  • the patch PA in which the washing solution is stored may further store the base material BS or the additive material AS to assist the washing.
  • the washing solution is stored in the patch PA, and the patch PA is contacted with and separated from the plate PL to remove impurities or residues of the plate PL, such as unbound antibody AB. Can be absorbed and removed.
  • the residue is a first antibody (AB) that does not form a specific bond with the antigen to be detected included in the sample (SA) or a second antibody that does not form a specific bond with the first antibody (AB). (AB) may be included.
  • the washing patch PA absorbs the residue because the washing patch PA contacts the plate PL, that is, the region of the plate PL where the specimen SA is located. ) May be formed, and the residue may be dissolved in the water film WF.
  • the residue dissolved in the water film WF is moved to the wash patch PA by moving the water film WF along the wash patch PA when the wash patch PA is separated from the plate PL. Can be absorbed).
  • the residue of the plate PL is removed using the washing patch PA as described above, the amount of the washing solution consumed is significantly smaller than that of the conventional ELISA method. Therefore, the diagnosis can be performed economically.
  • the substrate (PA) patch (PA) can be separated at an appropriate time to prevent overreaction, more accurate detection results can be obtained.
  • the immunodiagnostic method according to the present embodiment may be performed using a cleaning patch (PA) that performs the cleaning.
  • the cleaning patch PA may store and deliver the cleaning solution to the patch PA.
  • a cleaning patch PA may be used to easily remove substances that interfere with the detection of the specific binding from the plate PL.
  • the cleaning patch PA performs specific binding with the first antibody (AB) or the first antibody (AB) that does not form a specific binding with the antigen to be detected included in the sample (SA).
  • a cleaning material for easily removing a substance that interferes with the detection of the specific binding such as a second antibody (AB) that does not form, may be stored and used to remove the substance.
  • the cleaning patch PA may store at least a portion of the cleaning material for easily removing the disturbing materials.
  • the cleaning material may be at least one of Tween 20, Triton X-100, and CHAPS.
  • the immunodiagnostic method according to the present embodiment may be performed using a buffer patch (PA).
  • the buffer patch PA may store a buffer solution and deliver a predetermined environment to the plate PL.
  • the buffer patch PA may store a buffer solution for smoothly performing each step of the immune diagnosis.
  • a hydrogen peroxide buffer solution may be used when detecting chemiluminescence.
  • the immunodiagnostic method according to the present embodiment may be performed using a reaction stopping patch (PA).
  • the reaction stop patch PA may store a stop solution for stopping the reaction of the substrate SU catalyzed by the enzyme and transfer the stop solution to the plate PL.
  • the patch PA may store a stop solution for stopping the reaction of the substrate SU. That is, the reaction stop patch PA may be prepared, and the reaction solution may be transferred to the plate PL using the reaction stop patch PA to stop the reaction of the substrate SU at a suitable time.
  • the stopping solution may store at least a portion of the stopping material for stopping the reaction of the substrate (SU).
  • the stopping material may be sulfuric acid.
  • the stopping patch PA may be selectively applied to separating the above-described substrate SU patch from the plate PL in performing the immunodiagnostic method according to the present embodiment. For example, in order to stop the reaction of the substrate SU, the stopping patch PA is brought into contact with the plate PL, or the stopping patch PA is brought into contact with the plate PL. Contacting the patch PA (eg, contacting the top surface of the substrate SU patch in contact with the plate PL), or separating the substrate SU patch from the plate PL. Can be performed.
  • FIG. 63 shows a patch (PA) storing an antibody (AB) pair as one embodiment of a patch (PA) according to the present application.
  • the patch PA may store an antibody (AB) pair or an antibody (AB) complex in which a primary antibody (AB) and a secondary antibody (AB) are bound.
  • the primary antibody (AB6) and the secondary antibody (AB7) may be delivered to the plate (PL) together.
  • the patch PA may store and deliver the patch PA together with the primary antibody AB6 and the secondary antibody AB7 (FIG. 64).
  • the primary antibody (AB6) and the secondary antibody (AB7) is stored together in one patch (PA) and delivered to the plate (PL) can be a faster and simpler diagnostic procedure.
  • the patch (PA) for storing the primary antibody (AB) and the secondary antibody (AB) can be prepared to include the primary antibody (AB) and the secondary antibody (AB).
  • the patch (PA) storing the primary antibody (AB) and the secondary antibody (AB) may include the patch (PA) storing the primary antibody (AB) and the secondary antibody (AB). It can be produced by contacting the patch PA.
  • the secondary antibody (AB) may specifically bind to the primary antibody (AB).
  • the secondary antibody (AB) and the primary antibody (AB) may be stored in the patch PA and coupled to each other, and then transferred to the plate PL.
  • the washing patch PA may be a conjugate of the primary antibody (AB) and the secondary antibody (AB) that do not bind to the protein or antigen to be detected.
  • it is easy to remove by the washing patch (PA) is a conjugate of the primary antibody (AB) and the secondary antibody (AB) that does not bind the protein or antigen to be detected. Can be.
  • the patch (PA) is separated from the plate (PL) is removed from the plate (PL) and the primary antibody (AB) that does not bind the protein or antigen to be detected and It may be a conjugate of the secondary antibody (AB).
  • the patch (PA) storing the antibody (AB) pair contacts the patch (PA) storing the primary antibody (AB6) and the patch (PA) storing the secondary antibody (AB7). It can be obtained by allowing it to be separated and separated. Acquiring the patch (PA) storing the antibody (AB) pair is achieved by contacting the patch (PA) storing the primary antibody (AB6) and the patch (PA) storing the secondary antibody (AB7). Substances stored in the patch PA become interchangeable and diffusion causes the primary antibody AB6 and the secondary antibody AB7 to be located in each patch PA, and the primary antibody AB6 and The secondary antibody (AB7) may be obtained by binding to each other.
  • the antibody (AB) that specifically reacts with the target protein (TP) and the reaction region in which the antibody (AB) is stored and the target protein (TP) is located and It may be an antibody (AB) storage patch (PA) comprising a net structure (NS) to contact and deliver a portion of the antibody (AB) to the reaction region.
  • the target protein (TP) is an antigen
  • the antibody (AB) is a primary antibody (AB) having specific binding to the antigen and a secondary having specific binding to the primary antibody (AB).
  • An antibody (AB) pair formed by binding an antibody (AB), and the antibody (AB) pair may specifically react with the antigen.
  • the patch PA may store an antibody (AB), a substrate (SU), a washing solution, a base material (BS) or an additive material (AS) required for immunodiagnosis as described above.
  • the above-mentioned materials, in particular, the additive material AS may be stored in the patch PA from the fabrication step of the patch PA, or may be stored in a separate medium, and the patch may be stored when the diagnosis is performed.
  • PA may be absorbed and stored.
  • the patch PA may absorb and store a substance such as an antibody (AB) or a substrate (SU) from a separate medium.
  • the separate medium may be a plate PL or paper. What is stored in the separate medium is coated on one surface of the plate PL, or applied to one surface of the plate PL and dried, absorbed by paper, or absorbed and dried on the paper. Can mean.
  • the patch PA according to the present application may absorb and store the antibody AB applied to the plate PL.
  • Absorbing the antibody (AB) applied to the plate (PL), the antibody (AB) is captured in the water film (WF) formed by the patch (PA) in contact with the plate (PL) and the patch (PA) ) May be performed by separating the water film WF with the patch PA by being separated from the plate PL.
  • FIG. 77 shows, as an embodiment of a patch PA according to the present application, that a patch PA absorbing and storing the antibody AB from a separate medium delivers the antibody AB to the plate PL. It is shown.
  • the patch PA according to the present application absorbs the antibody AB from the plate PL3 to which the antibody AB is applied, and applies the sample SA to the absorbed antibody AB. It can be transferred to the plate PL4.
  • the lower surface of the patch PA contacts the plate PL4 on which the specimen SA is applied while the plate PL3 on which the antibody AB is applied is brought into contact with the upper surface of the patch PA. Can be performed.
  • the patch PA may transfer a substance (ie, a transfer target material) stored in a separate medium (ie, medium) to a plate or other external region.
  • a substance ie, a transfer target material
  • substances such as antibodies (AB) and substrates (SU) stored in separate media can be delivered to a separate plate.
  • the patch may store a liquid material capable of dissolving a transfer target material stored in the separate medium.
  • a liquid material capable of dissolving a transfer target material stored in the separate medium.
  • the patch may store water or an aqueous solution.
  • the patch may provide the water or the aqueous solution to the separate medium.
  • a substance stored in the separate medium can be delivered to the plate. This mechanism can be understood to provide a moist environment to the separate media and the plate by the patch.
  • one surface of the patch PA may be in contact with an external medium in which the material is stored.
  • a surface opposite the surface in contact with the patch of the external medium may be in contact with a plate or other external region.
  • the patch may transfer the material stored in the external medium to the plate.
  • the external medium may be a material having absorbency or permeability.
  • the external medium may be paper.
  • the external medium may be a material storage medium in the form of a mesh.
  • an immunodiagnostic method using the transcription patch may be provided. Specifically, in the immunodiagnostic method for detecting the target protein from the sample to be diagnosed by using a patch that includes a net structure to form microcavities, and can handle a liquid substance in the microcavities in the immunodiagnostic method Contacting the patch with a medium in which an antibody that specifically reacts with a target protein is stored, and contacting the patch with a reaction region in which the target protein is located, wherein the medium is in contact with the patch.
  • An immunodiagnostic method may be provided in which at least a portion of the antibody stored in is absorbed into the patch. In this case, when the patch contacts the reaction region, at least a portion of the antibody absorbed by the patch may be movable to the reaction region.
  • contacting the medium with the patch may include contacting one surface of the medium with the patch, and contacting the patch with the reaction region may include reacting one surface that is not in contact with the medium of the patch. Contact with the area.
  • an antibody delivery kit using the transcription patch may be provided. Specifically, a reaction in which an antibody that specifically reacts with a target protein forms a stored medium and fine cavities, the contact with the medium absorbs a portion of the antibody stored in the medium, and the target protein is located.
  • An antibody delivery kit may be provided comprising an antibody delivery patch in contact with a region to deliver at least a portion of the absorbed antibody to the reaction region.
  • 57 is a flowchart illustrating an immunodiagnostic method by direct ELISA as an example of an immunodiagnostic method according to the present application.
  • Immune diagnosis according to the present embodiment using the plate (PL) and the patch (PA), can be performed directly by ELISA.
  • the immunodiagnostic method according to the present embodiment may include fixing the sample (SA) to the plate (PL) and delivering the antibody (AB) using the patch (PA) to the plate (PL). have.
  • the fixing of the sample SA to the plate PL may be to dry and fix the sample SA to be diagnosed on the plate PL.
  • the fixing of the sample SA to be diagnosed on the plate PL may be to fix the fluid sample SA or a tissue section to be diagnosed on the plate PL.
  • Delivering the antibody (AB) using the patch (PA) to the plate (PL), the plate (PL) in contact with the patch (PA) in which the antibody (AB) is stored in contact with the plate (AB) (PL) may be to pass.
  • the contact of the patch PA with the plate PL and the matter of the antigen and the antibody AB are as described above with respect to the indirect ELISA.
  • the enzyme (AB) may be attached to the antibody (AB) by direct ELISA.
  • the enzyme attached to the antibody (AB) may be HRP or AP.
  • Delivering the antibody (AB) using the patch (PA), the step of contacting the patch (PA) to the plate (PL) and the step of separating the patch (PA) from the plate (PL) It may include. Contacting the patch PA with the plate PL and separating from the plate PL may allow the antibody AB to be selectively delivered. In other words, when the antigen to which the antibody (AB) specifically binds is immobilized on the plate (PL), the antibody (AB) may be selectively transferred from the patch (PA) to the plate (PL). have.
  • the antibody (AB) (hereinafter, the surplus antibody) that has moved to the plate (PL) without forming a specific binding is transferred to the patch (PA) as the patch (PA) is separated from the plate (PL).
  • the excess antibody (AB) is absorbed into the patch (PA), the excess antibody (AB) is dissolved in the water film (WF) formed by the patch (PA) in contact with the plate (PL), the patch ( When the PA is separated from the plate PL, the water film WF may be carried by moving along with the patch PA.
  • the surplus antibody (AB) can be removed from the plate (PL) only by separation of the patch (PA) as described above, the surplus material (ie, surplus antibody (AB)) that was essential to perform the conventional ELISA ) May be omitted.
  • the washing process of the surplus material which has been conventionally performed, requires pouring and rinsing a large amount of the washing solution to remove the surplus material not participating in specific binding from the plate PL.
  • the patch PA of the present application can be separated by capturing excess material just by contacting and separating the plate PL, the washing process is unnecessary. Therefore, the reagent can be used more efficiently, and the performance of the ELISA can be made simple.
  • the patch PA may deliver the antibody AB5 to the reaction region.
  • the antibody (AB5) may be attached with an identification label such as an enzyme. Delivery of the antibody AB5 to the plate PL by the patch PA is such that the patch PA contacts the plate PL and the antibody through the water film WF formed near the contact area. AB5 may be achieved by being movable to the plate PL or the reaction region of the plate PL.
  • the delivery of the antibody (AB5) to the plate (PL) is due to the specific binding of the antibody (AB5) and the target protein (TP) contained in the sample (SA), particularly the sample (SA). Can be.
  • the immunodiagnostic method according to the present embodiment may further include providing a substrate SU to the plate PL using the patch PA.
  • Providing the substrate SU using the patch PA may contact the patch PA in which the substrate SU is stored in the plate PL to transfer the substrate SU to the plate PL. It may be.
  • the substrate SU may be ABTS or TMB.
  • the substrate (SU) may be catalyzed by the enzyme to generate a product (PD), and the product (PD) may serve as a label of the specific binding to be detected.
  • the providing of the substrate SU to the plate PL using the patch PA in which the substrate SU is stored may include applying the patch PA in which the substrate SU is stored to the plate PL. Contacting and separating the patch PA in which the substrate SU is stored from the plate PL. The contact time of the patch PA in which the substrate SU is stored and the plate PL may be controlled by contacting and separating the patch PA in which the substrate SU is stored in the plate PL. In other words, the patch PA in which the substrate SU is stored may be separated from the plate PL at a suitable time.
  • the immunodiagnosis may be performed in a manner of detecting a product (PD) by a chemical reaction of an enzyme catalyzed substrate (SU), wherein if the reaction is not stopped at a suitable time, the product (PD) ) May be excessively produced, making it difficult to quantitatively detect the product (PD).
  • the reaction may be stopped at an appropriate time point by separating the patch PA in which the substrate SU is stored from the plate PL as described above. Measurement errors due to one overreaction can be eliminated.
  • the specific binding is quantitatively detected by measuring the product (PD) produced up to the proper time point. can do.
  • the patch PA stores the substrate SU and the substrate SU as a reaction area in which the sample SA or the sample SA is located on the plate PL.
  • Providing the substrate SU to the plate PL by the patch PA is such that the patch PA contacts the plate PL to form the substrate SU through the water film WF formed near the contact area.
  • the SU may be formed by being movable to the plate PL or the reaction region of the plate PL.
  • the substrate SU may be produced or converted into the product PD by a chemical reaction catalyzed by an enzyme attached to the antibody AB located on the plate PL.
  • the immunodiagnostic method according to the present embodiment may further include absorbing the residue by using the washing patch PA.
  • Absorbing the residue using the washing patch PA may contact the washing patch PA with the plate PL to absorb the residue.
  • Absorption of the residue using the washing patch PA may include: an antibody that does not specifically bind the absorption patch PA to the plate PL to specifically bind to at least a portion of the fixed sample SA. AB) may be absorbed.
  • the washing patch PA may absorb excess material that does not participate in the target specific binding in detecting the target specific binding.
  • Absorption of the residue by using the washing patch PA may include: delivering the antibody AB using the patch PA to the plate PL, and then applying the patch PA to the plate PL. ) May be performed prior to providing the substrate SU.
  • Absorption of the residue by using the washing patch PA may replace the washing using the washing solution in the conventional immunodiagnostic procedure.
  • the conventional washing process is performed in a manner in which a person directly removes surplus material using a large amount of washing solution, so that the solution is wasted, manpower is required, and the specific binding formed may be affected.
  • the washing is performed using the patch PA as in the present application, since the specific binding formed is not caused by the flowing water stream, there is a merit that the consumption of the washing solution is significantly reduced. .
  • the immunodiagnostic method according to the present embodiment may include deterge of substances that interfere with the detection of the specific binding.
  • the immunodiagnostic method according to the present embodiment may further include providing a predetermined environment to the plate PL.
  • the immunodiagnostic method according to the present embodiment may include stopping the reaction in the plate PL.
  • the details of the washing step, providing the environment and stopping the reaction can be applied similarly to the indirect ELISA described above.
  • the immunodiagnostic method according to the present embodiment may further include detecting an antigen specifically bound to the delivered antibody (AB). Detecting the antigen specifically bound by the antibody (AB) may be to detect a product (PD) generated by the reaction of the enzyme (AB) and the substrate (SU). In this case, detecting the product (PD) may be implemented by measuring color development due to the reaction, light emission due to the reaction, or measuring fluorescence due to the reaction.
  • PA patch
  • a method for performing diagnosis, and the like may be similarly applied in performing an immunodiagnosis by direct ELISA.
  • providing the substrate (SU) or delivery of the primary antibody (AB) or the secondary antibody (AB), etc. all of which must always be performed using a patch (PA) It is not.
  • Some of the steps of providing the substrate (SU) or delivering the primary antibody (AB) or the secondary antibody (AB) are in the liquid to solution state of the substrate (SU), primary antibody (AB) or secondary
  • the antibody (AB) may be replaced by a form for delivering the plate (PL).
  • 67 is a flowchart illustrating an immunodiagnostic method by sandwich ELISA as an example of an immunodiagnostic method according to the present application.
  • BAB bottom antibody
  • SA sample
  • TP target protein
  • the immunodiagnosis according to the present embodiment may be performed by a sandwich ELISA using a plate PL and a patch PA.
  • fixing the antibody (AB) to the plate (PL), applying a sample (SA) to the plate (PL), patch (PA) to the plate (PL) may include the step of delivering the antibody (AB) using.
  • Fixing the antibody (AB) to the plate (PL) may be to fix the antibody (AB) in a dried state. Fixing the antibody AB to the plate PL may be performed using a coating buffer solution. Fixing the antibody AB to the plate PL may be to form a thin film on the plate PL. The thin film may be fixed by being prepared in advance and attached to the plate PL. Fixing the antibody (AB) may be to fix the FC region of the antibody (AB) in contact with the plate (PL).
  • the immobilized antibody (AB) may be an antibody (AB) that specifically binds to a target antigen to be detected.
  • Coating the sample SA on the plate PL may apply the liquid body fluid.
  • applying the sample (SA) to the plate (PL) may be to apply any one of the blood, urine and cell suspension.
  • an antibody ie, a bottom antibody (BAB)
  • BAB bottom antibody
  • SA sample
  • Fixing the bottom antibody (BAB) may be to fix the FC region of the antibody (AB) in contact with the plate (PL).
  • the target protein TP included in the sample SA may be bound to the bottom antibody BAB.
  • the shape of the figure presenting the target protein and the like does not include an indication of the properties of the target protein and the like.
  • Delivering the antibody (AB) using the patch (PA) to the plate (PL) can be performed by the indirect method to the direct method or indirect method.
  • delivering the antibody (AB) to the plate (PL) by using the patch (PA) may be to specifically bind to the antigen to be detected and deliver the antibody (AB) to which the enzyme is attached.
  • Delivering the antibody (AB) using the patch (PA) to the plate (PL) the step of delivering the primary antibody (AB) using the patch (PA) to the plate (PL) and the plate (PL) may be to deliver a secondary antibody (AB) using the patch (PA).
  • the primary antibody (AB) using the first patch (PA) to the plate (PL) in the indirect ELISA described above Delivering the secondary antibody (AB) using the second patch (PA) to the plate (PL) and delivering the antibody (AB) using the patch (PA) in the above-described direct ELISA. Steps may apply mutatis mutandis.
  • FIGS. 70 and 71 show part of an immunodiagnostic method by sandwich ELISA according to an embodiment of the present application.
  • immunoassay by sandwich ELISA is performed by using a patch (PA) for storing an antibody (AB8) having an identification label, such as an enzyme, to deliver the antibody (AB8) to the plate (PL).
  • PA patch
  • AB8 an antibody having an identification label, such as an enzyme
  • Delivery of the antibody AB8 to the plate PL may be performed by contacting and separating the patch PA from the plate PL.
  • the non-target protein NTP included in the sample SA may be absorbed into the patch PA.
  • NTP non-target protein
  • the patch (PA) is in contact with the plate (PL) or the specimen (SA) to form a water film (WF) near the contact area
  • the non-target protein (NTP) may be carried out by being captured by the water film (WF).
  • the immunodiagnostic method providing the substrate (SU) to the plate (PL) using the patch (PA), absorbing the residue using the washing patch (PA), the specificity Deterge the substances that interfere with the detection of the binding, provide a predetermined environment in the plate PL, stop the reaction in the plate PL and the delivered antibody (AB) At least some of the steps of detecting the antigen specifically bound to.
  • the details of each step can be applied similarly to the indirect ELISA described above.
  • the immunodiagnosis by sandwich ELISA may include providing a substrate SU to the plate PL using a substrate SU patch.
  • the substrate SU provides the substrate SU, the water film WF is formed in the vicinity of the contact area by contacting the patch PA in which the substrate SU is stored to the plate PL, and through the formed water film WF.
  • the substrate SU may be performed by being movable to the plate PL.
  • the substrate SU may be converted into a product PD or a product PD through a chemical reaction catalyzed by an enzyme attached to the antibody AB located on the plate PL.
  • Immune diagnostics of the present application can be performed to detect multiple targets simultaneously.
  • a plurality of targets causing one disease may be detected simultaneously, or a plurality of targets causing a plurality of diseases may be detected at the same time.
  • multiple antigens involved in leukemia can be detected simultaneously.
  • target proteins (TP) that cause a plurality of different diseases such as leukemia and Zika virus can be detected simultaneously.
  • the simultaneous detection may mean that a plurality of targets may be detected in one plate PL, in addition to the detection of each target at some same time interval.
  • Detecting the plurality of targets at the same time may be performed differently depending on each method of performing the above-described immunodiagnostic method.
  • a method of detecting a plurality of targets in accordance with an execution method will be described. The overlapping contents with those described in the above embodiments will be omitted.
  • the immunodiagnostic method may include placing a sample (SA) in a reaction region (S200) and delivering the antibody (AB) to the reaction region (S300).
  • the target protein (TP) is a plurality
  • the plurality of target proteins (TP) includes a first target protein (TP) and a second target protein (TP)
  • the patch (PA) is the first target A first antibody (AB) that specifically reacts with the protein (TP)
  • a first antibody (AB) that specifically reacts with the second target protein (TP) may be stored.
  • the target protein (TP) is a plurality
  • the patch (PA) for storing the antibody (AB) is a plurality
  • the plurality of target proteins (TP) is the first target protein (TP) and the second target protein ( TP)
  • the plurality of patches PA include a first patch PA and a second target protein (AB) for storing a first antibody (AB) that specifically reacts with the first target protein (TP).
  • the immunodiagnostic method for detecting a plurality of targets fixing the sample (SA) to the plate (PL), the plurality of the plate (PL) Delivering the primary antibody (AB) of the kind and the delivery of the secondary antibody (AB) using a patch (PA) to the plate (PL).
  • the fixing of the sample SA to the plate PL may be applied similarly to the embodiment of the immunodiagnostic method by the indirect ELISA described above. However, when performing an immunodiagnostic for detecting a plurality of targets by indirect ELISA as in the present embodiment, the sample SA is distributed as one region on the plate PL or as a plurality of divided regions. Can be distributed.
  • Delivering a plurality of types of primary antibody (AB) to the plate (PL), may be to deliver a plurality of types of primary antibody (AB) stored in one patch (PA) to the plate (PL).
  • Delivering a plurality of types of primary antibody (AB) to the plate (PL) may be to deliver a plurality of types of primary antibody (AB) using a plurality of patches (PA) to the plate (PL).
  • Delivering the plurality of primary antibodies (AB), the first patch (PA) for storing the first primary antibody (AB) and the second patch (PA) for storing the second primary antibody (AB) By using may be to deliver the first primary antibody (AB) and the second primary antibody (AB) to the plate (PL).
  • delivering the plurality of types of primary antibodies (AB) may be performed by delivering the primary antibodies (AB) using a plurality of patches (PA) respectively storing the plurality of types of primary antibodies (AB). It may be.
  • the plurality of regions includes a first region and a second region, and delivering the primary antibody (AB) to the plurality of regions includes delivering a first primary antibody (AB) to the first region and the first region.
  • the second primary antibody (AB) may be delivered to two regions. In other words, delivering the primary antibody AB to the plurality of regions may be such that the plurality of patches PA deliver different primary antibodies AB to the plurality of regions.
  • Delivering a plurality of types of primary antibodies (AB) by using a plurality of patches (PA) to the plate (PL) the plurality of patches (PA) are sequentially contacted to the plate (PL), the sequence At least a portion of the regions in contact with each other may overlap to transfer the primary antibody (AB) stored in each patch (PA) to the plate (PL).
  • the delivery of the secondary antibody (AB) to the plate (PL) using the patch (PA) is specific for the first type of primary antibody (AB) using the patch (PA) to the plate (PL). It may be to deliver a second secondary antibody (AB) having a specific binding to the first secondary antibody (AB) having a positive binding and the second type of primary antibody (AB).
  • the delivery of the secondary antibody (AB) to the plate (PL) by using the patch (PA) has a specific binding to the plurality of primary antibodies (AB) to the plate (PL), respectively It may be to deliver a plurality of types of secondary antibody (AB).
  • Delivering the secondary antibody (AB) to the plate (PL) by using the patch (PA) is a common binding to the plurality of primary antibodies (AB) to the plate (PL) in common It may be to deliver a kind of secondary antibody (AB).
  • the secondary antibody (AB) is a plurality of types of secondary antibodies (AB) having specific binding to each type of the plurality of types of primary antibodies (AB) used for diagnosis or used for the diagnosis. There may be one type of secondary antibody (AB) having species specific binding to a plurality of types of primary antibodies (AB) in common.
  • Delivering the secondary antibody (AB) to the plate (PL) by using the patch (PA), the plurality of or one type of secondary antibody (AB) stored in the plurality of patches (PA) to the plate (PL) It may be to deliver.
  • the patch (PA) is the secondary antibody (AB) in the divided plurality of regions of the plate (PL) ) May be delivered.
  • the delivery of the secondary antibody (AB) to the plurality of regions may be to deliver the plurality of types of secondary antibodies (AB) to the plurality of regions, respectively.
  • the method of performing an immunodiagnosis to detect a plurality of targets may include providing a substrate SU using a patch PA. Details of providing the substrate SU using the patch PA are as described above in the Examples of Immunodiagnostic Methods by Indirect ELISA. However, a plurality of substrates SU may be used. In other words, one type of substrate (SU) may be used when one type of secondary antibody (AB) is used, and one type of substrate (SU) may be used when plural types of secondary antibody (AB) are used. It is possible to use a plurality of substrates (SU).
  • the method for performing an immunodiagnosis to detect a plurality of targets may include determining whether each of the plurality of targets exists from the plate PL. Details are as described above in the Examples of the method of immunodiagnostic by indirect ELISA.
  • the secondary antibody Delivering (AB) may be one patch (PA) delivers the secondary antibody (AB) to each region at the same time.
  • PA the secondary antibody
  • a plurality of types of primary antibodies AB are delivered to a plurality of divided regions of the plate PL
  • one kind of the secondary antibodies AB may be delivered to a plurality of regions.
  • one patch (PA) may be used to deliver the secondary antibody AB to a plurality of regions, or a plurality of small patches PA corresponding to each region may be used. If one patch (PA) is used, the diagnosis will be simplified. However, if a plurality of small patches (PA) are used, there is no risk of material transfer through the patch (PA). It will be possible.
  • delivering the secondary antibody (AB) means a plurality of kinds of secondary antibodies (AB) having specific binding to each of the plurality of primary antibodies (AB), It may be to deliver to the plate (PL) through one patch (PA).
  • PA patch
  • the plurality of types of secondary antibodies (AB) having specific binding to each of the plurality of types of primary antibodies (AB) can be detected according to different identification labels.
  • the delivery of the secondary antibody (AB) may include a kind of secondary antibody (AB) having specific binding properties in common to the plurality of types of primary antibodies (AB), and one patch (PA). It may be to pass through the plate (PL). This method can be used to determine the presence or absence of a disease when the antigen to which the plurality of primary antibodies (AB) specifically reacts is the cause of the same disease.
  • the secondary antibody (AB) is specific for the first secondary antibody (AB) and the second type of primary antibody (AB) having specific binding to the first type of primary antibody (AB).
  • a second secondary antibody (AB) having a binding ability, and the delivery of the secondary antibody (AB), the first secondary antibody (AB) to the first region located on the plate (PL) It may be to deliver and deliver the second secondary antibody (AB) to the second region located on the plate (PL).
  • delivering the secondary antibody (AB) may include placing a plurality of types of secondary antibodies (AB) having specific binding to each of the plurality of types of primary antibodies (AB) on the plate (PL). It may be to deliver to each of a plurality of divided regions. At this time, the detection target may be different for each divided region to facilitate the acquisition of a result.
  • the immunodiagnostic method for detecting a plurality of targets by using the above-described direct ELISA, the immunodiagnostic method for detecting a plurality of targets, the fixing of the sample (SA) to the plate (PL) and the plate (PL) Delivering a plurality of types of antibodies (AB) using the patch (PA).
  • Fixing the sample (SA) on the plate (PL) can be applied similarly to the method of performing an immunodiagnosis by the above-described direct ELISA.
  • the sample SA is distributed as one region or divided into a plurality of regions on the plate PL. It can be distributed as.
  • the plurality of kinds of antibodies AB When the plurality of kinds of antibodies AB are delivered to the plate PL by using the patch PA, the plurality of kinds of antibodies AB may have a first antibody (AB) having specific binding to the first antigen. ) And a second antibody (AB) having specific binding to the second antigen.
  • the first enzyme attached to the first antibody (AB) and the second enzyme attached to the second antibody (AB) may be heterologous enzymes.
  • the plurality of antibodies AB may be transferred to the plate PL using the patch PA on the plate PL. Delivering the plurality of types of antibodies (AB), the first antibody (AB) having a specific binding to the first antigen and the second antibody (AB) having a specific binding to the second antigen. It may be. In addition, the delivery of the plurality of types of antibodies (AB) may be the delivery of a plurality of types of antibodies (AB) each having specific binding to a plurality of different antigens (ie, a plurality of detection targets). . The plurality of types of antibodies AB may be attached with different types of identification elements. The plurality of types of antibodies (AB) may have a plurality of types of enzymes attached thereto.
  • the delivering of the plurality of types of antibodies AB may include delivering the plurality of types of antibodies AB stored in one patch PA to the plate PL.
  • the plurality of types of antibodies AB may be delivered to the plate PL by using a plurality of patches PA.
  • the plurality of patches PA may include a first patch PA and a second patch PA, and the plurality of patches PA may be used to deliver a plurality of types of antibodies AB using the plurality of patches PA.
  • the one patch (PA) delivers the antibody (AB) to the plurality of divided regions of the plate (PL) It may be.
  • the plurality of regions may include a first region and a second region, and the delivering of the antibody AB to the plurality of regions may be performed by using the first patch PA for storing a first antibody AB.
  • the second antibody (AB) may be delivered to the second region by using the second patch PA that delivers the antibody AB to the first region and stores the second antibody AB.
  • the delivering of the antibody (AB) to the plurality of regions may be such that the plurality of patches (PA) deliver different antibodies (AB) to the plurality of regions, respectively.
  • the antibody (AB) stored in each patch (PA) may be to deliver to the plate (PL).
  • a step of providing a substrate SU using a patch PA or determining whether each of the plurality of targets exists from the plate PL is determined. It may include the step. Details of each step can be applied similarly to those described above in connection with embodiments of the immunodiagnostic method by direct ELISA.
  • the immunodiagnostic method for detecting a plurality of targets using the sandwich ELISA described above fixing the antibody (AB) to the plate (PL), the specimen (PL) on the plate (PL) And applying the antibody (AB) using the patch (PA) on the plate (PL).
  • the fixing of the antibody AB to the plate PL may include fixing a plurality of types of antibodies AB to the plate PL.
  • the plurality of types of antibodies (AB) include a first antibody (AB) having specific binding properties to a first antigen to be detected and a second antibody (AB) having specific binding properties to a second antigen to be detected ) May be included.
  • the plural kinds of antibodies (AB) may be plural kinds of antibodies (AB) each having specific binding properties to plural kinds of antigens to be detected.
  • the first antibody AB may be fixed to a first region located on the plate PL, and the second antibody AB may be fixed to a second region located on the plate PL.
  • the plurality of kinds of antibodies AB may be fixed to a plurality of divided regions located on the plate PL.
  • the plurality of types of antibodies AB may be fixed to one region located on the plate PL.
  • the applying of the sample SA to the plate PL may include applying the sample SA to a region where the antibody AB of the plate PL is fixed.
  • the coating of the sample SA may be to apply the liquid sample SA.
  • the immunodiagnosis may be performed in a state in which the sample SA is applied in a liquid state, and fixed after the application in a liquid state to perform a diagnosis.
  • the application of the sample SA to the plate PL may be to apply the sample SA to one area positioned on the plate PL, and the first area to be located on the plate PL. And applying the sample SA to a second area.
  • the application of the sample SA to the plate PL may be to apply the sample SA to one region positioned on the plate PL, and the divided plurality may be disposed on the plate PL. It may be to apply the specimen (SA) to the area of each.
  • Applying the antibody (AB) using the patch (PA) to the plate (PL) is an immunodiagnostic method for detecting a plurality of targets using the above-described direct ELISA and for detecting a plurality of targets using an indirect ELISA It can be applied similarly in the immunodiagnostic method.
  • applying the antibody (AB) to the plate (PL) using the patch (PA) the plate (PL) in the immunodiagnostic method for detecting a plurality of targets using the above-described direct ELISA It can be applied similarly to the step of delivering a plurality of types of antibodies (AB) using the patch (PA).
  • the step of applying the antibody (AB) to the plate (PL) using the patch (PA), a plurality of types on the plate (PL) in the immunodiagnostic method for detecting a plurality of targets using the indirect ELISA described above Delivering the primary antibody (AB) of and can be applied similarly to the step of delivering the secondary antibody (AB) using the patch (PA) to the plate (PL).
  • the method of performing an immunodiagnosis to detect a plurality of targets by the sandwich ELISA providing a substrate (SU) using a patch (PA) or determining the presence or absence of each of the plurality of targets from the plate (PL). It may further comprise the step.
  • the details of each step can be applied similarly to those described above in connection with an embodiment of an immunodiagnostic method by sandwich ELISA and an immunodiagnostic method for detecting multiple targets by indirect or direct ELISA.
  • a plurality of targets can be detected at one time.
  • the plurality of targets to be detected may be configured with a predetermined target set or selectively configured for each diagnosis.
  • the diagnosis can be accelerated and various controls can be obtained.
  • the plurality of targets are selectively configured at each diagnosis, the patient-specific diagnosis can be performed, and a wider range of diseases that can be examined by one diagnosis can be expected.
  • the immunodiagnostic method according to the present embodiment may be performed using a plate PL having a plurality of unit regions.
  • the plate PL may have a plurality of unit regions and receive material from the patch PA for each unit region.
  • the plate PL may include a plurality of divided regions, that is, a plurality of unit regions.
  • the unit regions may be arranged in a mosaic form on the plate PL.
  • the unit regions may be disposed parallel to the plate PL in one direction.
  • the unit regions may be disposed in a form corresponding to the plurality of patches PA or the clusters of patches PA.
  • the plurality of unit areas may be divided / partitioned.
  • the unit regions may include a first region and a second region.
  • the first region and the second region may have a similar polarity, and the first region may have a different polarity from a third region other than the first region or the second region. Accordingly, the first area and the second area may be distinguished from each other.
  • the first area and the second area may have different heights from the bottom surface of the third area and the plate PL, and thus the first area and the second area may be distinguished from each other. .
  • the unit regions include a first unit region and a second unit region, wherein an antibody (AB) that binds specifically to a first antigen is fixed to the first unit region, and specific to the second unit region.
  • the antibody (AB) that binds to the target may be immobilized.
  • the first antigen and the second antigen may be different from each other.
  • Each unit region may have an antibody (AB) fixed thereto that specifically binds to each other antigen.
  • the unit regions to which the different antibodies (AB) are bound may be used to detect different antigens by sandwich ELISA.
  • each unit region includes a first unit region and a second unit region, and the first unit region includes a first unit region.
  • the patch PA may contact and the second unit PA may contact the second unit area.
  • the first patch PA and the second patch PA may be different from each other.
  • Each of the unit regions may be in contact with a different patch PA.
  • the unit patches PA constituting the patch PA cluster and the unit regions positioned on the plate PL may correspond to each other in arrangement, shape, and the like.
  • Each of the patches PA and each of the unit regions may be appropriately matched to obtain a desired result.
  • the immunodiagnostic method according to the present embodiment may be performed using a plurality of patches PA.
  • the plurality of patches PA may store materials and deliver materials to the plate PL.
  • the plurality of patches PA may store different materials and transfer materials stored in each patch PA to the plate PL.
  • the plurality of patches PA may store an antibody (AB) that specifically binds to different antigens.
  • the plurality of patches PA may store secondary antibodies AB that specifically bind to different primary antibodies AB.
  • the plurality of patches PA may store a substrate SU in which a reaction is induced by different enzymes.
  • the plurality of patches PA may form a patch PA cluster.
  • the plurality of patches PA may be a cluster composed of unit patches PA storing different materials.
  • the cluster may be used to deliver material to the plate PL at the same time.
  • the plurality of unit patches PA constituting the patch PA cluster may be manufactured in a standardized form.
  • the patch (PA) cluster may be manufactured in the form of a cartridge.
  • the configuration of a plurality of unit patches (PA) constituting the patch (PA) cluster can be changed according to the purpose.
  • the patch (PA) cartridge may be particularly useful when a user wants to implement a customized diagnosis for performing diagnosis by changing a target disease for each individual.
  • the unit patches PA constituting the patch PA cluster may be arranged in a mosaic form.
  • the unit patches PA constituting the patch PA cluster may be arranged in parallel in one direction.
  • a patch (PA) cluster including a plurality of patches (PA) may be provided.
  • a patch (PA) cluster may be provided comprising a plurality of antibody (AB) storage patches (PA).
  • the antibody (AB) storage patch (PA) is an antibody (AB) that specifically reacts with the target protein (TP) and the It may comprise a net structure (NS) to form microcavities in which the antibody (AB) is stored.
  • the plurality of antibody (AB) storage patches (PA) includes a first antibody (AB) storage patch (PA) and a second antibody (AB) storage patch (PA), and the first antibody (AB) storage
  • the target protein (TP) to which the first antibody (AB) stored in the patch (PA) specifically reacts is specifically reacted to the second antibody (AB) stored in the second antibody (AB) storage patch (PA). It may be different from the target protein (TP).
  • the immunodiagnostic method according to the present embodiment may be performed using a patch (PA) for storing a plurality of kinds of substances.
  • the patch PA may store a plurality of materials and transfer the same to the plate PL.
  • the patch PA may store a first antibody (AB) that specifically binds to a first antigen and a second antibody (AB) that specifically binds to a second antigen.
  • the patch PA may also bind to a first antibody (AB) that binds specifically to a first primary antibody (AB) and a second antibody (AB) that binds specifically to a second primary antibody (AB).
  • the patch PA may store a plurality of types of antibodies AB that specifically bind to a plurality of types of antigens.
  • the patch PA may store a plurality of types of antibodies AB that specifically bind to a plurality of types of primary antibodies AB.
  • the antibody (AB) storage patch (PA) is provided as an antibody (AB) and a net structure (NS) that specifically react with the target protein (TP), and the target protein (TP) It may include a network structure (NS) in contact with the located reaction region to deliver a portion of the stored antibody (AB) to the reaction region, wherein the target protein (TP) is a plurality, the plurality of targets Protein (TP) comprises a first target protein (TP) and a second target protein (TP), wherein the antibody (AB) specifically reacts with the first target protein (TP) (AB) And a second antibody (AB) that specifically reacts with the second target protein (TP).
  • TP network structure
  • the method of performing an immunodiagnosis of the present application may include applying the sample SA to the plate PL or applying the sample SA to the plate PL to fix the sample SA.
  • the application of the sample SA to the plate PL may be to coat the sample SA in a monolayer to a thin film.
  • the application of the sample SA to the plate PL to fix the sample SA may be performed by smearing the sample SA into a monolayer or a thin film.
  • the effective surface area of the sample SA smeared on the plate PL and the patches PA contacted to the plate PL is determined. Can be maximized.
  • the response area can be implemented very simply as compared with the conventional immunodiagnostic methods in which the distribution area of the sample (SA) is complicatedly designed and diagnosed.
  • the immunodiagnostic method according to the present application may include detecting the presence or absence of a target protein (TP) from a sample (SA).
  • the immunodiagnostic method according to the present application may include detecting the presence or absence of a target antigen from a sample (SA). Detecting the presence or absence of the target protein (TP) or the target antigen may mean quantitatively measuring the amount of the target protein (TP) or the target antigen included in the sample (SA).
  • the immunodiagnostic method may include detecting an antigen (that is, a target antigen) to which the antibody (AB) specifically binds, and detecting the antigen includes the antibody (AB) or the antibody ( It may be to detect a chemical reaction of the substrate (SU) catalyzed by an enzyme attached to another antibody (AB) that specifically binds AB) or a product (PD) according to the chemical reaction.
  • an antigen that is, a target antigen
  • detecting the antigen includes the antibody (AB) or the antibody
  • It may be to detect a chemical reaction of the substrate (SU) catalyzed by an enzyme attached to another antibody (AB) that specifically binds AB) or a product (PD) according to the chemical reaction.
  • detecting the chemical reaction of the substrate (SU) catalyzed by an enzyme attached to the antibody (AB) may be performed by a method of measuring colorimetric.
  • the detection of the product (PD) by colorimetry can be understood as follows.
  • Enzymes attached to the antibody (AB) (enzyme attached to the secondary antibody (AB) in the indirect method) can convert the substrate (SU) into a colored precipitate.
  • a stain may be formed, and the formed stain may be colorized and detected.
  • the DAB can generate a brown precipitate by the HRP.
  • the enzyme when the enzyme is HRP and the substrate (SU) is 4CN, the 4CN may generate a purple precipitate by the HCN.
  • the enzyme when the enzyme is AP and the substrate (SU) is BCIP (5-Bromo-4-chloro-3-indolyl phosphate) BCBC can generate a purple precipitate by the AP.
  • the detecting of the product PD may be performed by detecting a precipitate such as brown or purple.
  • the coloration may be performed by colorimetrically measuring the color using a spectrophotometer.
  • the colorizing may be performed by detecting light emitted from the light source and passing through the plate PL.
  • the coloration can be carried out in a manner to measure absorbance.
  • a plate PL having a flat bottom and a transparent surface may be used.
  • detecting the chemical reaction of the substrate (SU) catalyzed by an enzyme attached to the antibody (AB) may be performed in a manner of detecting luminescence.
  • Detecting the light emission may be to detect the light emission according to the chemical reaction of the substrate (SU).
  • detecting the light emission may be to detect light generated by using luminol as a substrate (SU).
  • Detecting the light emission may be performed using an X-ray film, a complementary metal-oxide semiconductor (CMOS) camera, or a charged coupled device (CCD) camera. Measuring the light emission may be performed by detecting light emitted from a solution on or at the bottom of the plate PL. Measuring the luminescence can be performed using a luminometer. In the case of measuring the light emission, preferably, the plate PL may be opaque black or opaque white.
  • CMOS complementary metal-oxide semiconductor
  • CCD charged coupled device
  • detecting the product (PD) according to the chemical reaction of the substrate (SU) catalyzed by the enzyme attached to the antibody (AB) may be performed in a manner to detect fluorescence.
  • the fluorescence may be detected by detecting fluorescence emitted from the fluorophore attached to the antibody AB (secondary antibody in the indirect method). Detecting the fluorescence may be to detect the fluorescence emitted from the product (PD) produced by the chemical reaction of the substrate (SU) catalyzed by the enzyme. More specifically, the enzyme can be catalyzed by cleaving a phosphate group from the substrate (SU) to generate a fluorescent product (PD), and detecting the generated fluorescence to perform the immunodiagnosis.
  • the detecting of the fluorescence may be performed by injecting light into the plate PL and measuring fluorescence emitted from the plate PL.
  • the fluorescence may be measured using a fluorometer with a filter attached thereto.
  • the plate PL may be opaque black or opaque white.
  • Detection of the results of the immunodiagnosis of the present application may be performed by an electrochemical method.
  • the change in electrochemical properties generated in the plate PL may be measured by the antibody AB specifically bound to the sample SA immobilized on the plate PL.
  • the patch PA may measure a change in electrochemical properties of the patch PA generated by delivering the antibody AB to the plate PL.
  • the substrate SU may be selectively provided to the plate PL.
  • Detection of the results of the immunodiagnostics according to the present application can be performed using imaging.
  • colorimetric (or colorimetric), luminescent or fluorescent images can be taken and used for diagnosis.
  • the images may be acquired as one for the entire area, or may be photographed for each part, or the photographed parts may be combined into one.
  • a distribution position of a target antigen / antibody (AB), a shape of a cell, a distribution of a target protein (TP) in a tissue, etc. may be obtained.
  • the location of the target protein (TP), the target antigen / antibody (AB), and the like and partial images of the target may be obtained.
  • Detection of the results of an immunodiagnostic according to the present application can be used to measure the amount of a particular protein contained in a sample (SA).
  • the immunodiagnosis in the present application may include measuring an amount of a target protein (TP) included in a sample to be diagnosed (SA) using an antigen-antibody response.
  • detecting the result of the immunodiagnosis according to the present application may include calculating the number of specific cells (ie, target cells) included in the sample to be diagnosed (SA).
  • Immune diagnosis according to an embodiment of the present application may be performed to detect a plurality of target proteins (TP) using the patch of the present application.
  • the step (S20) for placing a sample (SA) to be diagnosed in the reaction region the step of delivering the first antibody to the reaction region (S30) and the reaction region It may include the step (S40) of delivering a second antibody to (FIG. 46).
  • Positioning the sample (SA) to be diagnosed in the reaction region (S20), can be applied similarly to other embodiments.
  • the details of delivering the first antibody or the second antibody to the reaction region can be applied similarly to other embodiments of the present application.
  • Delivering the first antibody to the reaction region (S30) may be to deliver the first antibody to the reaction region using a patch for storing the first antibody that specifically reacts with the first target protein. .
  • the delivering of the second antibody to the reaction region (S40) may be to deliver the second antibody to the reaction region by using a patch that stores a second antibody that specifically reacts with the second target protein. .
  • the immunodiagnostic method according to the present embodiment may further include a step S50 of detecting the first target protein TP and the second target protein TP (FIG. 81).
  • detecting the first target protein specifically binds to the first target protein (TP).
  • Detecting the first fluorescence detected from the fluorescent label attached to the first antibody, and detecting the second target protein (TP), the agent specifically bound to the second target protein (TP) 2 may be to detect the second fluorescence detected from the fluorescent label attached to the antibody.
  • the wavelength band in which the first fluorescence is detected may be different from the wavelength band in which the second fluorescence is detected.
  • the immunodiagnostic method according to the present application further includes detecting the first target protein (S51) after delivering the first antibody to the reaction region (S30), wherein the reaction region After the step of delivering the second antibody to (S40), the step of detecting the second target protein (TP) (S53); may further comprise a.
  • detecting the first target protein TP may include detecting first fluorescence detected from a fluorescent label attached to the first antibody specifically bound to the first target protein TP.
  • the second target protein TP may be detected by detecting a second fluorescence detected from a fluorescent label attached to the second antibody specifically bound to the second target protein TP.
  • the wavelength band in which the first fluorescence is detected is at least partially overlapped with the wavelength band in which the second fluorescence is detected, and the detecting of the second fluorescence is performed after the second antibody is delivered to the reaction region.
  • the fluorescence detected from (SA) may be compared with the fluorescence detected from the sample (SA) before delivering the second antibody to the reaction region.
  • 83 and 84 schematically illustrate a part of a case of detecting a plurality of target proteins (TP) in the immunodiagnostic method according to the present application.
  • TP target proteins
  • the target protein TP included in the specimen may include a first target protein TP1 and a second target protein TP2.
  • the first antibody when the first antibody is delivered to the reaction region (S30) and the first target protein TP1 is detected (S51), the first antibody is bound to the first target protein TP1.
  • the first fluorescence can be detected from the fluorescent label attached to the first antibody (FIG. 83).
  • detecting the second fluorescence includes fluorescence detected from the sample after delivering the second antibody to the reaction region, and fluorescence detected from the sample before delivering the second antibody to the reaction region. In comparison, the detection of the second target protein (TP2) may be performed.
  • SA sample to be diagnosed
  • cell counting may be performed on a sample SA applied to an object having a planar reaction region such as a plate PL or a sample SA fixed and applied.
  • a planar reaction region such as a plate PL or a sample SA fixed and applied.
  • a cell counting method may include fixing a sample (SA) to a plate (PL) and using a patch (PA) to store an antibody (AB) that specifically reacts with a target cell. And delivering the antibody (AB) to the plate (PL) and obtaining the number of the target cells included in the sample (SA).
  • the fixing of the sample SA on the plate PL may be applied similarly to the above-described methods of immunodiagnosis.
  • an antibody (AB) having specific binding to some proteins constituting the target cell may be delivered to the plate (PL) through the patch (PA).
  • Acquiring the number of target cells included in the sample (SA) may be performed by a method of detecting fluorescence labeled on the antibody (AB).
  • Obtaining the number of target cells may be performed by a method of detecting a chemical reaction of a substrate (SU) catalyzed by an enzyme attached to the antibody (AB) or a product (PD) produced by the chemical reaction.
  • acquiring the number of target cells included in the sample SA may be performed by acquiring an image of a region in which the target cells are distributed on the plate PL. Acquiring the number of target cells may be performed by acquiring an image of a region in which the sample SA is distributed and detecting the target cells from the image. In other words, acquiring the number of target cells included in the sample SA may acquire only the numerical information of the cells from the intensity of the measured signal or the like, and may include an image of an area where the target cells are distributed or the image of the target cells. Images may also be acquired.
  • counting the number of cells may be performed simultaneously on a plurality of target cells.
  • to be performed simultaneously may mean that the number of target cells can be calculated using one plate PL.
  • the simultaneous operation may mean that the sample may be performed on a sample SA positioned in one reaction zone.
  • the patch PA may store a first antibody AB that specifically reacts with a first target cell and a second antibody AB that specifically reacts with a second target cell.
  • the first antibody AB may be labeled with a first fluorescent substance
  • the second antibody AB may be labeled with a second fluorescent substance.
  • the fluorescence emitted from the first fluorescent material and the second fluorescent material may have different wavelength bands detected.
  • the cell counting method described above may be performed using a plurality of patches (PA) for a plurality of target cells.
  • PA patches
  • the first included in the sample (SA) Obtaining the number of target cells.
  • the second antibody (AB) After delivering the second antibody (AB) to the plate (PL), it may comprise the step of obtaining the number of the second target cells contained in the sample (SA).
  • SA the number of the second target cells contained in the sample (SA).
  • the number of the first target cells and the second target cells included in the sample (SA) can be obtained after the first antibody AB and the second antibody AB are transferred to the plate PL.
  • Obtaining the number of the first target cells or the number of the second target cells may be applied similarly to obtaining the number of target cells included in the sample SA in the above-described cell calculation method.
  • the fluorescent material labeled on the first target cell and the fluorescent label labeled on the second target cell may be homogeneous.
  • a wavelength band in which the fluorescent material labeled on the first target cell is detected and a wavelength band in which the fluorescent material labeled on the second target cell are detected may overlap.
  • the cell counting method for the plurality of target cells according to the embodiment may be performed using a plurality of patches (PA).
  • PA patches
  • the plurality of target cells includes a first target cell and a second target cell
  • the plurality of patches PA includes a first patch PA and a second antibody (AB) for storing a first antibody (AB). It may include a second patch (PA) for storing the AB).
  • the first antibody (AB) specifically binds to a protein specifically expressed in the first target cell
  • the second antibody (AB) specifically binds to a protein specifically expressed in the second target cell. can do.
  • the immunodiagnosis of the present application can be performed by an immunodiagnostic device.
  • the immunodiagnostic apparatus may include a plate (PL) support part 200, a patch control part 300, and a reaction detection part 400.
  • the immunodiagnostic apparatus according to the present embodiment includes a net structure NS that forms microcavities, and uses a patch capable of storing a liquid substance SB in the microcavities, to be diagnosed subject SA. Diagnosis can be performed by detecting the target protein (TP) from.
  • TP target protein
  • the plate PL support unit 200 may support the plate PL in which the reaction area is located and the sample SA to be diagnosed is located in the reaction area.
  • the patch controller 300 reacts the patch PA to deliver the antibody AB to the reaction region using the patch PA storing the antibody AB that reacts specifically with the target protein TP. You can control the relative position to the area.
  • the response detector 400 may detect a specific reaction between the antibody AB and the target protein TP in order to diagnose a target disease.
  • the immunodiagnostic apparatus may further include a control unit 100.
  • the immunodiagnostic device includes a net structure NS that forms microcavities, and uses a patch PA capable of storing a liquid substance SB in the microcavities, from a sample to be diagnosed SA. Diagnosis can be performed by detecting the target protein (TP).
  • TP target protein
  • the immunodiagnostic device may specifically react with the target protein (TP), a plate (PL) support for supporting a plate (PL) on which a reaction area is located and a sample (SA) to be diagnosed is located in the reaction area.
  • the patch control unit for controlling a relative position of the patch PA to the reaction region to deliver the antibody AB to the reaction region using the patch PA storing the antibody AB, and to diagnose the target disease. It may include a reaction detection unit for detecting a specific reaction of the antibody (AB) and the target protein (TP).
  • 79 illustrates an example of the patch controller 300 in an embodiment of the immunodiagnostic apparatus 10 according to the present application.
  • the patch control unit 300 may include a patch selection module 310 and a contact control module 330.
  • the patch selection module 310 may select a control target patch PA.
  • the patch selector selects a control target patch (PA) to store a patch (PA) storing a primary antibody (AB), a patch (PA) storing a secondary antibody (AB), and a substrate (SU). At least one of a patch PA, a washing patch PA, or a patch cluster may be selected.
  • the contact control module 330 may control the contact state between the selected patch PA and the reaction region. Controlling the contact state may be controlling a relative position of the patch PA with respect to the reaction region.
  • the reaction detector 400 may be any one of an optical detector and an electrochemical detector.
  • the reaction detector may include an image acquisition module.
  • the reaction detector may include a camera module.
  • the reaction detector may acquire an image for each part of the reaction region.
  • the reaction detector may collect the acquired partial images.
  • Immunodiagnosis is to diagnose using antigen-antibody binding, but the application of the patch (PA) is not limited to this, but can also be applied to similar fields of diagnosis using specific interactions.
  • PA patch
  • body fluid refers to liquid components obtained from the human body, such as blood, urine, cerebrospinal fluid (DSF), tears, and runny nose, and also includes pus and effusion.
  • DSF cerebrospinal fluid
  • Specific interactions used in the diagnosis encompass various forms of chemical and biochemical reactions. For example, there may be a method of detecting a factor indicating the presence of a disease by using a diagnostic reagent, or determining the normality by measuring the concentration of a specific component in the blood. In addition, for example, a method using a reaction between an enzyme and a substrate and a method for measuring the activity of an enzyme may be used.
  • a diagnosis result may be obtained by detecting the specific interaction.
  • colorimetry, colorimetric detection, fluorescence detection and electrochemical detection methods can be usefully utilized.
  • the method of performing the immunodiagnosis using the patch (PA) described above can be similarly performed in the clinical chemistry diagnosis.
  • the clinical chemistry diagnosis may also be performed in the plate PL, and as performed using the patch PA, the same effects as in the immunodiagnosis may be expected.
  • the diagnosis may be performed by applying or spreading the sample SA on the plate PL. Therefore, since the effective contact surface area is maximized, a diagnosis result having sufficient effectiveness can be expected even with a small amount of sample (SA).
  • SA sample
  • the clinical chemical diagnosis can be performed using the patch PA, it is very easy to add and remove a reagent involved in the detection from the plate PL in which the target detection process is performed. Therefore, the reagent used for detection and the time required for diagnosis can be saved.
  • Clinical chemistry diagnostics can be used for hormone detection.
  • SA sample
  • PA patch
  • SA sample
  • the sample SA applied to the plate PL may be blood or urine.
  • To guide the sample SA to the plate PL may be to fix and apply the sample SA to the plate PL.
  • Some of the reagents involved in the hormone detection may be pre-located in the plate PL.
  • Detecting the target hormone may be to detect cortisol according to the Poter-Silber method. Detecting the target hormone is Follicle-Stimulating Hormone (HSP), Adrenal Cortical Stimulating Hormone (ACTH: adrenocorticot-ropin hormone), Growth Hormone (GH), Thyroid-Stimulating (TSH) hormone) and thyroid hormone (T4: Thyroid hormone).
  • HSP Follicle-Stimulating Hormone
  • ACTH Adrenal Cortical Stimulating Hormone
  • GH Growth Hormone
  • Thyroid-Stimulating (TSH) hormone Thyroid-Stimulating hormone
  • T4 Thyroid hormone
  • Detecting the target hormone may be a reaction of a target hormone present in the sample SA and a reagent delivered to the sample SA. Detecting the reaction may be performed using any one of colorimetry, fluorescence detection, luminescence detection and electrochemical sensor.
  • Clinical chemistry diagnostics can be used to measure blood cholesterol. At this time, an enzymatic method can be used.
  • Clinical chemistry diagnosis according to an embodiment of the present application, applying a sample (SA) to the plate (PL), deliver a reagent by using a patch (PA) on the plate (PL), the sample (SA) By measuring cholesterol content.
  • SA sample
  • PA patch
  • the sample SA applied to the plate PL may be serum.
  • Application of the sample SA to the plate PL may be to apply and fix the sample SA to the plate PL.
  • a portion of a reagent used for detecting cholesterol may be previously located in the plate PL.
  • the reagent delivered to the plate PL using the patch PA may be an enzyme reagent.
  • the enzyme reagent may include at least some of 4-aminoantipyrine (4AA: 4-aminoantipyrine), peroxidase, cholesterol esterase and cholesterol oxidase.
  • Detecting the cholesterol content may be to measure the absorbance.
  • the clinical chemistry diagnosis may be performed using an enzyme method in the measurement of triglyceride in blood.
  • the sample SA may be applied to the plate PL, the reagent may be delivered to the plate PL using the patch PA, and the content of triglyceride may be measured from the sample SA.
  • the sample SA applied to the plate PL may be serum.
  • the reagent delivered to the plate PL using the patch PA may be an enzyme reagent.
  • the enzyme reagent may include at least some of glycerol kinase, lipase, phenol, 4-aminoantipyrine, peroxidase, and pyruvate kinase.
  • Detecting the cholesterol content may be to measure the absorbance.
  • Clinical chemistry diagnosis can be used to measure the concentration of a specific component contained in the sample (SA).
  • SA sample
  • the specific component to be measured may be blood glucose concentration, that is, blood sugar concentration.
  • a sample SA is applied to the plate PL, a reagent is delivered to the plate PL using a patch PA, and the reaction is performed by the reagent.
  • the reaction is performed by the reagent.
  • the enzyme method can be used.
  • the sample SA applied to the plate PL may be any one of whole blood, plasma, serum, urine, cerebrospinal fluid, and pleural effusion.
  • the reagent delivered to the plate PL may include any one of glucose oxidase and hexokinase.
  • Measuring the blood glucose may be performed by measuring the result of the dye reaction of the reaction with the reagent. Measuring blood glucose may be performed using reflectance photometry or using electrochemical measuring means.

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Abstract

La présente invention concerne un procédé de diagnostic d'immunité qui exécute un diagnostic immunologique par utilisation d'un timbre qui stocke un anticorps, un procédé de diagnostic d'immunité selon un aspect de la présente invention utilisant un timbre, celui-ci comprenant une structure de filet ayant des micro-cavités et pouvant stocker une matière liquide dans les micro-cavités, afin d'exécuter un diagnostic par détection d'une protéine cible dans un échantillon qui est soumis à un diagnostic, le procédé comprenant les étapes suivantes : le positionnement, dans une zone de réaction, de l'échantillon qui doit être soumis au diagnostic; le transfert d'un anticorps vers la zone de réaction par utilisation d'un timbre stockant un anticorps qui réagit spécifiquement avec la protéine cible.
PCT/KR2017/002028 2016-02-23 2017-02-23 Nécessaire de fourniture d'anticorps, timbre de stockage d'anticorps, dispositif et procédé de diagnostic d'immunité l'utilisant WO2017146504A1 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP17756842.5A EP3428642B1 (fr) 2016-02-23 2017-02-23 Nécessaire de fourniture d'anticorps, timbre de stockage d'anticorps, dispositif et procédé de diagnostic d'immunité l'utilisant
US16/079,451 US11385144B2 (en) 2016-02-23 2017-02-23 Antibody-providing kit, antibody-containing patch, method and device for immunoassay using the same
JP2018562489A JP6828986B2 (ja) 2016-02-23 2017-02-23 抗体提供キット、抗体含有パッチ、これを使用する免疫学的測定のための方法及びデバイス
CA3015599A CA3015599C (fr) 2016-02-23 2017-02-23 Necessaire de fourniture d'anticorps, timbre de stockage d'anticorps, dispositif et procede de diagnostic d'immunite l'utilisant
EP23172629.0A EP4235144A3 (fr) 2016-02-23 2017-02-23 Nécessaire de fourniture d'anticorps, timbre de stockage d'anticorps, dispositif et procédé de diagnostic d'immunité l'utilisant
CN201780025367.0A CN109073632B (zh) 2016-02-23 2017-02-23 提供抗体的试剂盒、储存抗体的贴片及使用其的免疫诊断方法和装置
US17/842,651 US20230009655A1 (en) 2016-02-23 2022-06-16 Antibody-providing kit, antibody-containing patch, method and device for immunoassay using the same

Applications Claiming Priority (16)

Application Number Priority Date Filing Date Title
US201662298959P 2016-02-23 2016-02-23
US62/298,959 2016-02-23
KR10-2016-0069936 2016-06-04
KR10-2016-0069937 2016-06-04
KR1020160069937A KR20170099738A (ko) 2016-02-23 2016-06-04 접촉식 염색 패치 및 그 제조 방법
KR1020160069936A KR20170099737A (ko) 2016-02-23 2016-06-04 접촉식 염색 패치 및 이를 이용하는 염색 방법
KR1020160069938A KR20170099739A (ko) 2016-02-23 2016-06-04 접촉식 염색 보조 패치, 그 제조 방법 및 이를 이용하는 염색 방법
KR10-2016-0069938 2016-06-04
KR10-2016-0095739 2016-07-27
KR1020160095739A KR20170099741A (ko) 2016-02-23 2016-07-27 테스트 키트
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KR1020160144551A KR20170099745A (ko) 2016-02-23 2016-11-01 진단 방법 및 이를 수행하는 기기
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KR1020170024389A KR102045070B1 (ko) 2016-02-23 2017-02-23 항체 제공 키트, 항체 저장 패치, 이를 이용하는 면역 진단 방법 및 장치
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US17/842,651 Division US20230009655A1 (en) 2016-02-23 2022-06-16 Antibody-providing kit, antibody-containing patch, method and device for immunoassay using the same

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6063029A (en) * 1992-12-07 2000-05-16 Hisamitsu Pharmaceutical Co., Inc. Diagnostic patch and method for diagnosis using the same
US20110070606A1 (en) * 2008-04-25 2011-03-24 Constitution Medical, Inc. Systems and methods for analyzing body fluids
US20110257666A1 (en) * 2008-10-17 2011-10-20 Sofradim Production Surgical patch
US20140038230A1 (en) * 2010-05-08 2014-02-06 Veridex, Llc A simple and affordable method for immunophenotyping using a microfluidic chip sample preparation with image cytometry

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6063029A (en) * 1992-12-07 2000-05-16 Hisamitsu Pharmaceutical Co., Inc. Diagnostic patch and method for diagnosis using the same
US20110070606A1 (en) * 2008-04-25 2011-03-24 Constitution Medical, Inc. Systems and methods for analyzing body fluids
US20110257666A1 (en) * 2008-10-17 2011-10-20 Sofradim Production Surgical patch
US20140038230A1 (en) * 2010-05-08 2014-02-06 Veridex, Llc A simple and affordable method for immunophenotyping using a microfluidic chip sample preparation with image cytometry

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BECK, MARKUS ET AL.: "On-chip Sample Preparation by Controlled Release of Antibodies for Simple CD 4 Counting", LAB ON A CHIP, vol. 12, no. 1, 7 January 2012 (2012-01-07), pages 167 - 173, XP055534042 *
See also references of EP3428642A4 *

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