WO2016182382A1 - Poste, utilisé pour un appareil de test, ayant un moyen de réaction et de détection intégré - Google Patents

Poste, utilisé pour un appareil de test, ayant un moyen de réaction et de détection intégré Download PDF

Info

Publication number
WO2016182382A1
WO2016182382A1 PCT/KR2016/005050 KR2016005050W WO2016182382A1 WO 2016182382 A1 WO2016182382 A1 WO 2016182382A1 KR 2016005050 W KR2016005050 W KR 2016005050W WO 2016182382 A1 WO2016182382 A1 WO 2016182382A1
Authority
WO
WIPO (PCT)
Prior art keywords
unit
sample
collecting member
belt
filling chamber
Prior art date
Application number
PCT/KR2016/005050
Other languages
English (en)
Korean (ko)
Inventor
김병철
문봉석
이영행
방주형
하남철
안재운
Original Assignee
바디텍메드(주)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020160058165A external-priority patent/KR101811786B1/ko
Application filed by 바디텍메드(주) filed Critical 바디텍메드(주)
Priority to ES16793028T priority Critical patent/ES2826865T3/es
Priority to EP16793028.8A priority patent/EP3296718B1/fr
Priority to CN201680039919.9A priority patent/CN107923839B/zh
Priority to US15/574,131 priority patent/US10670499B2/en
Publication of WO2016182382A1 publication Critical patent/WO2016182382A1/fr
Priority to US15/817,089 priority patent/US10871474B2/en

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14—Suction devices, e.g. pumps; Ejector devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/13—Moving of cuvettes or solid samples to or from the investigating station

Definitions

  • the present application relates to an analysis apparatus for detecting a specific component included in a biological sample or the like.
  • the sample and the reagents used to inspect the sample are not influenced by external factors, and using the correct amount each time is very important for obtaining accurate and reproducible results.
  • samples and reagents may be exposed to the outside, so it is necessary to effectively prevent contamination due to exposure of these samples and reagents, and to use the correct amount to ensure the accuracy of the inspection.
  • the test for detecting and reading / analyzing the reaction result is carried out accurately and quickly under one integrated system, thereby reducing test time and test cost, and thus including the steps and inputs included in the test. It is also necessary to reduce the cost.
  • the technical problem of the present invention is that the reaction of the sample and the reagents and the result read / analyze can be performed in one system in an integrated, accurate and rapid manner. It is possible to perform reaction, detection, and analysis on several samples at the same time, and to provide equipment capable of corresponding and application analysis for different fluorescence assays using a plurality of light sources having different wavelengths.
  • the analyte test station of the sample according to the present application is used in a reaction and detection integrated cuvette having a collecting member atmospheric chamber, a sample filling chamber, a reagent filling chamber, and a detecting unit, on which a collecting member is placed.
  • An analyte test station of a sample comprising: a housing having an inlet and outlet through which a plurality of the cuvets are introduced into and out of the station, the housing having a space such that the plurality of cuvettes are laterally side by side; It is provided inside the housing and moves the plurality of the cuvette back and forth, and moves the collecting member to the left and right to position the collecting member on any one of the plurality of the cuvette, the collecting member to move up and down
  • a first drive unit which reacts a sample of the sample filling chamber with a reagent of the reagent filling chamber and injects the reaction result into the detection unit; And an optical reader provided on the forward and backward movement paths of the cuvette and reading or analyzing a detection result of the reaction result.
  • a second driving unit which moves the optical reader left and right to position the optical reader on any one of the plurality of cuvettes.
  • the first driving unit wherein the sampling member is located in any one of the sample filling chamber, the reagent filling chamber and the detection unit of any one of the plurality of cuvets while moving the plurality of cuvets back and forth.
  • a forward and backward moving unit positioned at a point;
  • a vertical movement unit coupled to the sampling member and configured to vertically move the sampling member in any one of the sample filling chamber, the reagent filling chamber, and the detection unit;
  • a left and right moving unit connected to the vertical moving unit and moving the collecting member and the vertical moving unit in a left and right direction so that the collecting member is positioned on any one of a plurality of the cuvets;
  • a pump unit that provides suction or discharge force when the sampling member is inserted into any one of the sample filling chamber, the reagent filling chamber, and the detection unit of any one of the plurality of cuvettes.
  • the front and rear movement unit, the holder is provided at a position corresponding to the inlet and outlet and the plurality of the cuvet is seated; Front and rear guide part for guiding the holder in the front and rear direction; And it may include a front and rear drive unit for applying a force in the front and rear direction to the holder.
  • the front and rear guide portion a horizontal support for supporting the holder to the housing; Front and rear guide rails formed on one of the horizontal support members in contact with the holder or the holder in contact with the horizontal supports; And a front and rear guide groove formed at another one of a portion of the horizontal support which is in contact with the holder or a portion of the horizontal support which is in contact with the horizontal support.
  • the front and rear drive unit a first connection bracket connected to the holder; An annular first belt to which the first connection bracket is fixed; A first motor provided at one side of the first belt to rotate the first belt; And a first driven pulley provided on the other side of the first belt to rotatably support the belt.
  • the plurality of front and rear movement units may be provided, and the plurality of front and rear movement units may be individually driven.
  • It may further include a removal unit disposed between the plurality of front and rear movement units to separate the collecting member from the vertical movement unit.
  • the removal unit includes a slider having a through hole penetrated in the up and down direction and slidable in one direction, wherein the slider slides when the collecting member is inserted into the through hole so that the collecting member is moved from the vertical moving unit. Can be separated.
  • the removal unit may include a jig having a sliding hole penetrating in the vertical direction and extending in the front and rear directions to form a path in which the slider slides, and the sampling member disposed below the jig and separated from the vertical movement unit to fall.
  • the treatment box may further include a spring disposed in the sliding hole to apply elasticity between the inner surface of the sliding hole and the slider to elastically bias the slider.
  • the holder may be formed in parallel with each other one or more mounting channels are provided so that one or more of the cuvette can be inserted and mounted.
  • the holder may be provided with a heater for applying heat to the holder, and a temperature sensor for sensing the temperature of the holder.
  • the vertical movement unit may include: a second connection bracket connected to the horizontal movement unit and movable in a horizontal direction and extending in a vertical direction; A vertical guide rail provided on the second connection bracket and extending in a vertical direction; An arm moved up and down along the vertical guide rail; And a vertical driving part connected to the second connection bracket and applying a vertical force to the arm.
  • the vertical drive unit may include: a third connecting bracket connected to the arm and moved up and down along the vertical guide rail; A second belt having a ring shape in which the third connection bracket is fixed and extending in the vertical direction; A second motor connected to the second connection bracket and provided at one side of the second belt to rotate the second belt; It may include a second driven pulley connected to the second connection bracket and provided on the other side of the second belt to rotatably support the second belt.
  • the left and right guide portion, the left and right guide rails are provided to extend in the left and right direction in the housing;
  • the second connecting bracket may be provided with left and right guide grooves engaged with the left and right guide rails.
  • the front and rear driving unit may include: a third belt having a ring shape in which the second connection bracket is fixed and extending in a left and right direction; A third motor provided at one side of the third belt to rotate the third belt; It may include a third driven pulley provided on the other side of the third belt to rotatably support the third belt.
  • the collecting member is positioned on any one of the plurality of cuvets by the left and right moving unit, and the collecting member is placed on the sample filling chamber of the cuvette by the front and rear moving unit. Is positioned and the collecting member is inserted into the sample filling chamber by the up and down moving unit to provide suction force to the collecting member, and the reagent filling chamber is positioned on the collecting member by the front and rear moving unit and the collecting member is The suction unit and the discharge force are repeatedly provided to the collecting member when the sample is inserted into the reagent filling chamber by the vertical moving unit, and the detection unit is positioned on the collecting member by the front and rear moving unit, and the collecting member is moved up and down. When inserted into the detection unit may provide a discharge force to the collecting member.
  • the pump unit may include a conduit formed through the arm; And a pump provided to the arm and applying a pump force to the collecting member through the pipe.
  • the second driving unit may include a fourth connection bracket to which the optical reader is connected; Second left and right guides for guiding the fourth connection bracket in a left and right direction; And a second front and rear driving unit applying a force in the left and right directions to the fourth connection bracket.
  • the second left and right guide portion may include: a second left and right guide rail extending in the left and right direction in the housing; It may include a second left and right guide portion provided in the fourth connecting bracket and having a second left and right guide groove engaged with the second left and right guide rail.
  • the second front and rear driving unit may include: a fourth belt having a ring shape in which the fourth connection bracket is fixed and extending in the left and right directions; A fourth motor provided at one side of the fourth belt to rotate the fourth belt; It may include a fourth driven pulley provided on the other side of the fourth belt to rotatably support the fourth belt.
  • the station according to the embodiment of the present invention described above may further include a display unit provided in the housing and displaying an analysis result by the optical reader.
  • the station according to the embodiment of the present invention described above may further include a chip inserting portion provided in the housing and into which a chip containing information of a sample filled in the sample filling chamber is inserted.
  • the station according to the embodiment of the present invention described above may further include a print output unit provided in the housing and configured to print and output the analysis result.
  • the station according to the embodiment of the present invention described above may further include a door provided in the housing to open and close the inlet and outlet.
  • the station according to the embodiment of the present invention described above, the printed circuit board provided in the housing; And a control unit mounted on the printed circuit board and controlling the first driving unit, the second driving unit, and the optical reader.
  • the optical reader may include a plurality of laser light sources and a plurality of filters to measure and analyze fluorescent signals having different wavelengths.
  • the cuvette further includes a barcode in which the type of analyte in the sample is encoded, the station further includes a chip and a chip inserting portion into which the chip is inserted, the barcode is interlocked with the chip, and the chip is the sample. It may contain information for driving the station according to the type of analyte.
  • the present application provides a method for testing analytes in a sample using a station according to the present application.
  • the station according to the present invention is a test apparatus having an integrated reaction and detection means, for example, a cuvette described below is inserted and used, and the station is used for the distribution of a sample, the reaction of a reagent with a sample, and the detection of the reaction product. Inspections for reading / analysis can reduce inspection time, improve inspection accuracy and reproducibility, as well as reduce the steps and costs involved throughout the inspection, accurately and quickly under one integrated system.
  • the station according to the present application has a holder having a plurality of mounting channels so that a plurality of cuvettes are coupled to one holder, a plurality of front and rear movement units for receiving and moving the holder is provided, thereby simultaneously multiple diagnosis in a system And analysis can be made. Therefore, various tests and diagnosis / analysis are performed quickly for accurate diagnosis at the place for examination and treatment, thereby saving time, cost and manpower.
  • the station according to the present application has a light source having a light source having a different wavelength from each other and an optical reader capable of measuring the fluorescence of the different wavelengths, respectively, it is possible to correspond and apply analysis for the different fluorescence analysis method.
  • the optical reader is configured to be movable left and right, inspection of each of the plurality of cuvettes may be performed.
  • the housing included in the station according to the present application can block the inflow of foreign matter to perform a more accurate sample inspection.
  • the optical reader is provided on the left and right movement paths of the cuvet together with the driving unit providing up and down and left and right movement forces, the sample inspection can be performed quickly and simply.
  • the pump unit included in the station according to the present application through the sampling member can accurately control the amount of the sample, reagent or reaction product upon inhalation or discharge.
  • the front and rear drive unit of the pulley-belt type included in the station according to the present application can prevent vibration and foreign substances due to friction generated when moving left and right, unlike the gear type can be made more accurate inspection.
  • the heater and the temperature sensor are provided in the holder, the sample contained in the sample filling chamber, the reagent contained in the reagent filling chamber, and the reaction product contained in the detector are maintained at the appropriate temperature required for inspection. Can be.
  • the analysis result can be immediately checked visually, thereby enabling quick inspection.
  • the chip inserting portion since the chip inserting portion is provided, it is possible to input more quickly and accurately than to input the information of the sample through the keyboard.
  • a test result may be provided as a document even without using a separate printer.
  • the door since the door is provided, the door may be closed during the inspection to prevent foreign matter from entering the inside of the housing, thereby performing more accurate inspection.
  • the entire process related to the inspection may be automatically performed.
  • the cuvette used in the station according to the present application may further include a barcode.
  • the barcode includes lot information of the analyte (item) and the cuvette.
  • the station further includes a chip interworking with the bar code, and can drive the station to perform an optimal inspection in accordance with a plurality of various analytes, thereby easily inspecting various analytes with one station. The reproducibility and reliability of the test can also be improved.
  • the barcode retrieves information through a barcode scanner that scans it.
  • FIG. 1A is a perspective view schematically showing a station according to an embodiment of the present invention.
  • FIG. 1B is a schematic perspective view of a station according to another embodiment of the present invention.
  • FIG. 2A is a perspective view schematically illustrating the inside of FIG. 1A in one direction.
  • FIG. 2A is a perspective view schematically illustrating the inside of FIG. 1A in one direction.
  • FIG. 2B is a perspective view schematically illustrating the inside of FIG. 1B in one direction.
  • FIG. 2B is a perspective view schematically illustrating the inside of FIG. 1B in one direction.
  • FIG. 3 is a perspective view schematically showing the inside of the station of FIG. 1 from another direction;
  • FIG. 4 is a perspective view schematically showing the inside of the station of FIG. 1 in another direction;
  • FIG. 5A is a perspective view schematically showing a part of the inside of the station of FIG. 2A;
  • FIG. 5B is a perspective view schematically showing a portion of the inside of the station by omitting.
  • FIG. 6 is a perspective view schematically showing in another direction by omitting a part of the inside of the station of FIG.
  • FIG. 7A is a schematic perspective view of a holder extracted from the station of FIG. 1; FIG.
  • FIG. 7B is a perspective view illustrating the coupling between the holder and the cuvette in the station of FIG. 1.
  • FIG. 7C is a view illustrating the front and top surfaces of the cuvette fixing member provided in the holder of FIG. 7B.
  • FIG. 7D is a cross-sectional view schematically illustrating the insertion state of the collecting member of the cuvette mounted on the holder and a sensor attached to the bottom surface of the holder for detecting the top, and a top view of the holder.
  • FIG. 7E is an enlarged perspective view of a sensor for detecting whether a cuvette is mounted on a rear surface of the holder and an enlarged view thereof.
  • FIG. 8 is a perspective view schematically illustrating an essential component of the first drive unit of the station of FIG. 1.
  • FIG. 9 is a perspective view schematically showing an essential component of the first drive unit of the station of FIG. 1.
  • FIG. 10 is a perspective view schematically illustrating an essential component of the first drive unit of the station of FIG. 1.
  • FIG. 11A is a perspective view schematically illustrating an essential component of the second driving unit in the station of FIG. 2A;
  • FIG. 11B is a schematic perspective view of the main component of the second drive unit of the station of FIG. 2B;
  • FIG. 12 is a schematic perspective view of an optical reader extracted from the station of FIG. 2A.
  • 13, 14 and 15 are side views schematically illustrating a process in which the cuvet is inserted into a holder of the front and rear movement unit.
  • FIG. 16 is a view illustrating a process of placing a sample filling chamber, a reagent filling chamber, and a detection unit of a cuvette in a collecting member coupled to an arm while the cuvette moves forward by a front and rear moving unit.
  • 17 and 18 are views illustrating a process of separating the collecting member from the arm.
  • 19A and 19B schematically show the process of separating the collecting member by the collecting member removing unit shown in FIGS. 17 and 18 and the size relationship between the inner groove of the hole and the outer diameter of the collecting member, respectively.
  • 20 is a view schematically showing the operation of the second drive unit of the station of FIG.
  • 21A and 21B are perspective views schematically showing a cuvette in which a station according to the present invention is used and a detection means used in mounting thereto.
  • 22 is a control block diagram of a main system of a station according to the present invention.
  • FIG. 23 is a block diagram of a station operating system according to the present invention.
  • 24 is a block diagram of a circuit of a cartridge heating system of a station according to the present invention.
  • 25 is a block diagram of a heater control circuit of a station according to the present invention.
  • 26 is a real picture of a station according to an embodiment of the present invention.
  • each member is exaggerated, omitted, or schematically illustrated for convenience and clarity of description.
  • the size and area of each component does not necessarily reflect the actual size or area.
  • FIG. 1 is a perspective view schematically showing a station according to an embodiment of the present invention.
  • FIGS. 5A, 5B and 6 are perspective views schematically showing a part of the inside of the station of FIG.
  • FIGS. 7 to 12 are perspective views schematically illustrating main components of the driving unit and an optical system or an optical reader, respectively, of the station of FIG. 1.
  • the station according to the invention is used with a test device, eg cuvette, with integrated reaction and detection means.
  • a test device eg cuvette
  • the cuvette used in the station according to the present invention is used to detect an analyte contained in a sample, and for detection, the reaction of the sample and the reagent may be performed and the analyte in the reaction result may be detected.
  • Detection herein refers to the development of the reaction product according to an appropriate method as described below, in order to determine the presence or amount of analyte included in the sample described below, the result being read at a station according to the present application. do.
  • test is used as a term encompassing both detection, analysis and reading.
  • sample refers to a substance that includes an analyte or analyte that needs to be detected, and a sample that can be used in the present invention is a liquid phase or a fluid similar to liquid.
  • the sample is a biological sample, and may be a body-derived body component such as whole blood, plasma, serum, urine, saliva, manure and cell extracts.
  • analyte refers to a compound to be analyzed in a sample, also referred to as a target, and includes proteins and nucleic acids, wherein the protein comprises natural or synthetic polypeptides and peptides, and the nucleic acid comprises natural or synthetic DNA. , RNA and cDNA.
  • a "reagent" is a substance suitable for detecting or analyzing an analyte as described above, and varies according to a specific analyte.
  • the reagent may be a predetermined antibody that reacts with various substances in the body component, for example, an antigen and the like. Or an antigen causing a reaction with the antibody, but is not limited thereto.
  • the station according to an embodiment of the present invention, the reaction unit and the detection unit 240 including the collecting member atmospheric chamber 210, the sample filling chamber 220, the reagent filling chamber 230, the collecting member 100 is placed
  • a station for inspecting a sample by inserting a cuvette 200 including the housing 300, a first drive unit 400, an optical reader 500, a second drive unit 600, and a removal unit. And 700.
  • Housing 300 included in the station according to the present application serves to block the inflow of foreign matter therein.
  • the housing 300 is provided with an inlet and outlet to which the cuvette 200 is inserted.
  • the cuvette 200 enters the inside of the housing 300 through the inlet and outlet, foreign matters to the sample filling chamber 220, the reagent filling chamber 230, and the detection unit 240 of the cuvette 200 through the housing 300. This entry is blocked so that more accurate sample inspection can be performed.
  • the first driving unit 400 is provided inside the housing 300, moves the mounted cuvette 200 back and forth, and moves the arm 423 to one. While moving from the cuvette to another cuvette and fastening the body taking member 100 to move up and down, the sample of the sample filling chamber 220 is reacted with the reagent of the reagent filling chamber 230 to inject the reaction result into the detection unit 240. In this way, it is automatically driven by the control unit.
  • the collecting member 100 used together with the first driving unit 400 according to the present invention has a tubular shape, and the diameter thereof is gradually smaller toward the end thereof, so that the end portion has a pointed shape.
  • the collecting member 100 may be formed of a flexible material to facilitate fixing to the arm 423 and detaching from the arm 423.
  • the collecting member 100 includes a disposable microtip (for example, a 2-1000 ⁇ l volume micropipette tip), which is used in conjunction with an arm 423 described below for dispensing a sample and / or reagent, and separate reagents. It can be used with equipment that does not have a supply device and means for cleaning contamination, thereby simplifying the operation of the equipment.
  • the collecting member 100 is seated in the atmospheric chamber 210 of the cuvette 200 (see FIG. 13), and when the inspection process starts, it is fastened to an arm 423 to be described later (see FIG. 15). Together with the pump unit 440 serves to aspirate or discharge for dispensing or dispensing a sample or reagent.
  • the cuvette 200 used in the station according to the present invention has an elongated shape, as shown in the figure, and the atmospheric chamber 210, the sample filling chamber 220, the reagent filling chamber 230, and the detection unit 240.
  • the cuvette 200 according to the present disclosure may include one or more.
  • the waiting chamber 210, the sample filling chamber 220, the reagent filling chamber 230 and the detector 240 is along the longitudinal direction so that the inspection process is performed while the sampling member 100 is linearly moved in the front and rear direction It can be formed sequentially.
  • the waiting chamber 210 is a place where the sampling member 100 rests and waits until the test is started
  • the sample filling chamber 220 is a place where a predetermined biological sample including an analyte to be tested is filled.
  • the reagent filling chamber 230 is a place where a reagent such as an antibody to react with a sample is filled
  • the detector 240 is a place including detection means for detecting a reaction product generated by reacting the sample with the reagent.
  • the cuvette 200 may further include a barcode or a QR code, which is used in conjunction with a chip described below inserted into a station.
  • Barcodes herein include, but are not limited to, UPC-A, UPC-E, EAN, Code 3 of 9, Interleaved 2 of 5, Code 128, UCC / EAN-128, Codabar, PostNet, Pharmacode, or PDF-417. Or, but not limited to, 1D barcodes or 2D barcodes.
  • the barcode or QR code encodes the type of analyte and the lot number of the cuvette according to the type of sample.
  • the detection unit 240 constituting the cuvette 200 is a cartridge 260 suitable for lateral flow analysis as shown in FIG. 20 and FIG. ) May be included.
  • Lateral flow analysis is a method of quantitatively or qualitatively examining a target analyte, eg, a particular nucleic acid or protein, contained in a sample. Chromatographic methods involving nitrocellulose membranes (development mediums) called bound strips are used to transfer analytes in the reaction product to detect specific nucleic acids or proteins in the sample through sequence specific hybridization or antigen antibody reactions. Way. For example, reference may be made to those disclosed in Korean Unexamined Patent Publication Nos. 2003-0065341, 2011-0007699, 2011-0127386, and Registration No. 1149357.
  • the cartridge for chromatographic analysis of the lateral flow method installed and used in the detection unit 240 according to the present application is as shown in Figs.
  • the cover member 30 and the base member 10 is included.
  • the reaction product is introduced into the well 12 through the inlet 32, and triangular capillary structures 35 and 37 are formed on the lower surface of the cover member 30 to transfer the reaction product from the well to the strip 20.
  • the base member 10 has a structure corresponding thereto.
  • the lateral flow analysis cartridge included in the cuvette 200 of the present application includes a base member 10; And a cover member 30 engaged with the base member 10, wherein the base member 10 includes a strip accommodating portion 18 for receiving a strip used for the lateral flow analysis, and the strip accommodating portion. And a sample well 12 formed at a position extending from one end of the end portion 18, wherein the cover member 30 corresponds to the strip receiving portion 18 when the base member 10 is filled with the cover member 30. And a sample inlet 32 formed in a portion corresponding to the sample well 12 and a measurement window 34 for detecting a reaction result formed in the sample, and may further include an air window 38 if necessary. .
  • the base member 10 or the cover member 30, or the base member 10 and the cover member 30 include capillary structures 35 and 37, and a capillary structure formed in the cover member 30. Is formed on the bottom surface adjacent to the sample inlet 32 of the cover member 30, and the capillary structure formed on the base member 10 is formed on the sample well 12. The sample inlet 32 is formed to be perpendicular to the well containing the reaction product. Covering the cover member 30 on the base member 10 is interlocked with each other at its interface via a fastening means including a locking jaw and an uneven protrusion 16, thereby substantially waterproof and aerosol-proof loop. It is an aerosol proof seal.
  • the strip receiving portion 18 includes a guide for receiving a plurality of strips to prevent the mounted strip from shaking, as well as to position the strip in a certain position.
  • the reaction product introduced into the inlet 32 is moved to the strip 20 through various methods.
  • the lateral flow cartridge may have a predetermined microchannel structure formed between the well formed at a position perpendicular to the inlet and the strip receiving portion 18, and the reaction product of the well is transferred to the strip by capillary action.
  • lateral flow is disclosed, but not limited thereto.
  • one end of the strip is in direct contact with the well to absorb a liquid sample into the strip to initiate lateral flow, but is not limited thereto.
  • the optical reader or optical system 500 included in the station according to the present invention is provided on the left and right movement paths of the cuvette 200, and generates data by reading the reaction result detected using the above-described cuvette 200, This is used to qualitatively and / or quantify specific analytes contained in the sample.
  • the analyte or a reagent for detecting the same may be labeled with one or more fluorescent materials emitting light at a specific wavelength, and according to the type of the fluorescent material, the optical reader 500 irradiates and emits light of a specific wavelength. It is optimized for reading or absorbance measurements of emitted light.
  • the optical reader 500 is provided on the front and rear movement paths of the cuvette 200 and is movable side by side in each row of cuvettes parallel to each other, two or more optical systems may be accommodated in the optical system.
  • the inspection of several cuvettes can be performed quickly and simply.
  • the optical system or the optical reader 500 may have one or more light sources, and if two or more, each light source may generate light having different wavelengths.
  • each light source may generate light having different wavelengths.
  • the fluorescence of different wavelengths can be measured respectively, or absorbance can be measured. Therefore, the range of application for the diagnostic test method can be extended and the sensitivity can be improved.
  • the optical reader 500 includes a first fluorescence measurement unit 501, a second fluorescence measurement unit 503, and an absorbance measurement unit 502, as shown in FIG. 12, and each fluorescence measurement unit is an LED.
  • a light source or a laser light source may be provided.
  • the optical reader 500 As shown in FIGS. 2A, 2B and 6, the optical reader 500 according to the present application is moved left and right by the second driving unit 600 provided in the housing 300 to be used among the plurality of cuvettes 200.
  • the optical reader 500 may be positioned on any one of the cuvets 200 to perform a sample inspection of the cuvette 200.
  • the second drive unit 600 is automatically driven by the control unit.
  • the first drive unit 400 includes a front and rear movement unit 410 for moving the cuvette 200 back and forth (before and / or after or the Y axis), and the specimen.
  • Vertical movement unit 420 for moving the collecting member 100 up and down (up and / or down or Z axis) for suction, mixing with the reagent and dropping of the reaction resultant, and the collecting member 100 from right to left ( Left and / or right, or X-axis) may include a left and right movement unit 430 to move, and a pump unit 440 to provide a suction force or a discharge force to the collecting member 100.
  • the front and rear movement unit 410 moves any one of the sample filling chamber 220, the reagent filling chamber 230, and the detection unit 240 to the point where the sampling member 100 is positioned while moving the cuvette 200 back and forth. Play a role.
  • the front and rear movement unit 410 may include a holder 411, a front and rear guide part 412, and a front and rear drive part 413 as illustrated in FIGS. 2A and 2B to 6. .
  • the holder 411 is provided at a position corresponding to the inlet and outlet of the housing 300, and the cuvet 200 is seated therein. For example, when the cuvette 200 is pushed into the holder 411 through the inlet and outlet (see FIG. 13), the cuvette 200 is fully inserted into and seated in the holder 411 (see FIGS. 2A and 2B). ).
  • the holder 411 has one or more cuvette mounting channels 411a formed so that one or more of the cuvets 200 may be inserted and mounted, respectively, and each channel may have a predetermined value.
  • Cuvette fixing members 411b and 411c are formed on the upper surface of each wall forming a boundary between channels and having an asymmetrical flange shape.
  • a plurality of mounting channels 411a having a slot-like shape are formed in the holder 411, and an upper portion of each of the mounting channels 411a fixes a cuvette inserted therein.
  • fixing members 411b and 411c protruding in the longitudinal direction of each channel are formed.
  • the fixing member may not only stably mount the cuvette 200 inserted into the channel without shaking, but may also read a barcode formed on the upper surface of the cuvette.
  • the holder 411 is provided with a plurality of channels, and thus a plurality of cuvettes can be inspected, but the cuvettes are not necessarily used for all channels.
  • the plurality of mounting channels 411a may be formed in plural to be parallel to each other so that the plurality of cuvets 200 may be arranged side by side in the lateral direction.
  • the front and rear guide portion 412 guides the holder 411 in the front and rear direction.
  • the horizontal support 412a and the front and rear guide rails 412b are provided. And, and may include a front and rear guide groove (412c).
  • the horizontal support 412a is provided between the housing 300 and the holder 411 as shown in FIGS. 2A and 2B to support the holder 411 to the housing 300.
  • the front and rear guide rails 412b may protrude to a portion of the horizontal support 412a that contacts the holder 411 as shown in FIGS. 2A, 2B, and 5, and although not illustrated, the horizontal guide rails 412b may be horizontal.
  • the front and rear guide grooves 412c are formed as shown in FIGS. 2A, 2B, and 5, and the front and rear guide grooves 412b are engaged with the front and rear guide rails 412b when the front and rear guide rails 412b are formed on the horizontal support 412a.
  • the 412c may be formed at a portion of the holder 411 that is in contact with the horizontal support 412a, or may be formed at a portion of the holder 411 that is in contact with the holder 411 so as to engage with the front and rear guide rails 412b. May be Accordingly, the holder 411 may be guided left and right without swinging through the front and rear guide parts 412.
  • the front-rear drive unit 413 applies a force in the front-rear direction to the holder 411.
  • the first connection bracket 413a and the first belt 413b, a first motor 413c, and a first driven pulley 413d.
  • the first connection bracket 413a connects the holder 411 and the first belt 413b.
  • the first belt 413b is fixed to the first connecting bracket 413a to transfer the power of the first motor 413c to the first connecting bracket 413a.
  • the first motor 413c is provided on one side of the first belt 413b to rotate the first belt 413b.
  • the first driven pulley 413d is provided on the other side of the first belt 413b to rotatably support the first belt 413b.
  • the front and rear drive unit 413 of the pulley-belt type unlike the gear type, it is possible to prevent vibration and foreign substances due to friction generated when moving left and right can be made more accurate inspection.
  • the front and rear movement unit 410 may be provided in plurality. That is, as illustrated in FIGS. 2A, 2B, and 5A and 5B, two front and rear movement units 410 may have a configuration in which they are arranged side by side in the left and right directions, respectively. In addition, the number is not necessarily limited to this.
  • the plurality of front and rear movement units 410 is provided, which means that the plurality of holders 411, the front and rear guide parts 412, and the front and rear drive parts 413 are provided.
  • the movement directions of the plurality of front and rear movement units 410 are all parallel to each other as the front and rear directions.
  • the inspection can be performed by using the cuvet 200 having different kinds of samples at the same time, the time required for analysis is shortened, and the convenience is increased.
  • two sets of holders 411 having three mounting channels are provided, so that a total of six cuvets 200 may be inspected at a time, and the number thereof may be determined at this time.
  • each of the front and rear movement unit 410 may be driven separately from each other to facilitate the inspection. That is, as shown in the drawing, even when there are two forward and backward movement units 410, the respective driving may be performed separately, and the inspection may be performed individually for each cuvette provided in each unit.
  • a removal unit 700 may be further disposed between the plurality of front and rear movement units 410 and separate the collection member 100 from the vertical movement unit 420. After the removal unit 700 uses the above-described collecting member 100, the collecting member 100 used from the vertical moving unit 420 to connect the new collecting member 100 to the vertical moving unit 420 ( 100).
  • the removal unit 700 will be described with reference to FIGS. 2A, 2B, 5A, 5B and 6 and 19A and 19B.
  • the removal unit 700 may have a through hole 701 penetrated in an up and down direction, and the through hole may include a slider 702 slidable in one direction.
  • the slider 702 is configured to be movable in a sliding manner in one direction, and has a through hole 701 penetrated in the vertical direction.
  • the slider 702 allows the size of the through hole to be adjusted according to the diameter of the tip and the end of the arm inserted in the sliding manner when the collecting member 100 is inserted into the through hole 701. Therefore, as shown in FIG.
  • the collecting member inserted at the end of the arm will be described later.
  • the outer diameter a of the groove formed on the lower surface of the through hole should be larger than the outer diameter b of the collecting member.
  • the removal unit 700 is positioned on the left and right movement paths of the collecting member 100 by the first left and right movement unit 430. Accordingly, when the use of the collecting member 100 is completed, the collecting member 100 is inserted into the through hole 701 after being positioned on the removal unit 700 using the first horizontal movement unit 430. Can be separated. As shown in FIG. 19A, a groove is formed along an edge of the through hole at a lower surface of the removal unit 700 in which the through hole 701 is located, and a part of the groove may be used to facilitate separation of the collecting member 100.
  • the protrusion 701b is formed.
  • the protrusion 701b is formed only on a part of the left or right side of the lower surface of the through hole, so that only a portion of the upper edge of the collecting part is loaded when the collecting member inserted through the through hole is separated or removed from the vertical movement unit 420. It becomes easy to separate.
  • an inner side of the groove of the bottom surface of the through hole may be formed along the circumference of the through hole, and an outer side thereof may be formed along the circumference of the through hole or in a straight line.
  • the removal unit 700 may further include a jig 706 having a sliding hole 704, a treatment box 708, and a spring.
  • the jig 706 has a sliding hole 704 penetrating in the vertical direction and extending in the front-rear direction to form a path in which the slider 702 slides. Accordingly, the slider 702 may be disposed in the sliding hole 704 formed in the jig 706 and may slide along the sliding hole 704.
  • the sliding hole 704 is configured to penetrate in the vertical direction so that the slider 702 may be exposed in the vertical direction.
  • the treatment box 708 is disposed below the jig 706 and is configured to drop the collection member 100 separated from the vertical movement unit 420.
  • a spring disposed in the sliding hole 704 may be further provided to elastically bias the slider 702 by applying elasticity between the inner surface of the sliding hole 704 and the slider 702. Accordingly, when there is no separate external force or operation signal, the slider 702 may maintain the standby state at one position.
  • the vertical movement unit 420 is coupled to the sampling member 100, any one of the sample filling chamber 220, the reagent filling chamber 230 of the cuvette 200, the detection unit 240 using a lateral flow chromatography In the collecting member 100 serves to move up and down. Therefore, the collecting member 100 may be inserted into one chamber or spaced apart from the chamber while being moved up and down by the vertical movement unit 420.
  • the vertical movement unit 420 may include a second connecting bracket 421, a vertical guide rail 422, an arm 423, and a vertical driving unit 424, as illustrated in FIGS. 4 and 9. ) May be included.
  • the second connection bracket 421 serves as a structure supporting the vertical movement unit 420 as a whole, and is provided to be connected to the first horizontal movement unit 430 which will be described later.
  • the vertical guide rail 422 is provided in the second connection bracket 421 and is provided to extend in the vertical direction.
  • one side of the arm 423 or the third connecting bracket 424a connected to the arm 423 corresponds to the corresponding shape. Grooves are formed.
  • Arm 423 (arm) is moved in the vertical direction along the upper and lower guide rails 422, the sampling member 100 at the end by the front and rear movement unit 410 and the vertical movement unit 420 when the sample inspection starts Are automatically combined.
  • the collection member serves to prevent the internal pressure from binding.
  • the collecting member and the coupling portion may be surrounded by a material having high adhesion, for example, a urethane-based rubber material in order to increase the contact force on the hard material surface.
  • the vertical driving unit 424 applies a vertical force to the arm 423.
  • the third driving bracket 424a, the second belt 424b, and the second belt 424b are provided.
  • Motor 424c, and second driven pulley 424d are provided.
  • the third connection bracket 424a is connected to the arm 423, one side is connected to the upper and lower guide rails 422, and one side is connected to the second belt 424b to be described later.
  • a portion corresponding to the upper and lower guide rails 422 is formed to be guided by the upper and lower guide rails 422.
  • the second belt 424b extends in the vertical direction and is fixed to the third connection bracket 424a to transmit power of the second motor 424c to the third connection bracket 424a.
  • the second motor 424c is provided on one side of the second belt 424b to rotate the belt 424b.
  • the second driven pulley 424d is provided on the other side of the second belt 424b to rotatably support the second belt 424b.
  • the pulley-belt type vertical driving unit 424 is provided, unlike the gear type, vibration and foreign substances due to friction generated when moving up and down can be prevented, so that more accurate inspection can be made.
  • the arm 423 can prevent the left and right shake while moving up and down to accurately move the sampling member 100 up and down.
  • a configuration in which the upper and lower guide rails 422 and the groove part are connected to each other may be additionally provided to further prevent vibration.
  • the first horizontal movement unit 430 is connected to the vertical movement unit 420 to move the vertical movement unit 420 and the collecting member 100 in the horizontal direction. Accordingly, the collecting member 100 may be positioned on any one of the plurality of cuvets 200 positioned side by side while being moved left and right by the first horizontal movement unit 430. . Therefore, sample analysis may be performed on the one cuvette 200.
  • the first left and right movement unit 430 may include a first left and right guide part 431 for guiding the second connection bracket 421 in a left and right direction; And a first left and right driving part 432 applying a force in a left and right direction to the second connection bracket 421.
  • the first left and right guide part 431 is provided in the first left and right guide rails 431a and the second connection bracket 421 provided to extend in the left and right direction in the housing 300 and the first left and right guides.
  • a first left and right guide portion 431b having left and right guide grooves engaged with the rail 431a may be included.
  • the first left and right guide rails 431a extend in the left and right directions in the housing, and are provided in parallel with a path in which the second connection bracket 421 moves left and right.
  • predetermined support members may be provided at both side ends of the first left and right guide rails 431a, respectively.
  • a member such as a predetermined bar extending between the support members is provided to provide the first left and right guide rails 431a, and one or more first left and right guide rails 431a are provided on the bar.
  • two first left and right guide rails 431a spaced apart from each other in the vertical direction and extending side by side are provided.
  • the second connecting bracket 421 is provided with a first left and right guide part 431b.
  • the first left and right guide part 431b includes a guide groove for engaging the first left and right guide rail 431a to allow the second connection bracket 421 to move along the first left and right guide rail 431a. .
  • the first left and right driving unit 432 applies a force in the left and right directions to the second connection bracket 421.
  • the third belt 432a and the third motor 432b. For example, as illustrated in FIGS. 2 and 10, the third belt 432a and the third motor 432b. ), And a third driven pulley 432c.
  • applying the force in the left and right directions to the second connection bracket 421, the force in the left and right direction with respect to the vertical movement unit 420 and the collecting member 100 is eventually connected to the second connection bracket 421. It can be taken to mean moving by adding.
  • the third belt 432a extends in the left and right direction and is fixed to the second connection bracket 421 to transfer the power of the third motor 432b to the second connection bracket 421.
  • the third motor 432b is provided at one side of the third belt 432a to rotate the third belt 432a.
  • the third driven pulley 432c is provided on the other side of the third belt 432a to rotatably support the third belt 432a.
  • the first left and right driving unit 432 of the pulley-belt type unlike the gear type, it is possible to prevent vibration and foreign substances due to friction generated when moving left and right can be made more accurate inspection.
  • the left and right shake can be prevented while being moved left and right to accurately move the sampling member 100 left and right.
  • a configuration in which the first left and right guide rails 431a and the first left and right guide parts 431b are connected to each other may be additionally provided to further prevent vibration.
  • the sampling member 100 may move horizontally at the same time as the vertical movement, and thus, among the plurality of cuvets 200 arranged side by side. After placing the collecting member 100 on any one of the cuvette 200, the inspection can be performed.
  • the pump unit 440 serves to provide suction force or discharge force when the sampling member 100 is inserted into any one of the sample filling chamber 220, the reagent filling chamber 230, and the detector 240 of the cuvette 200. Do it.
  • the collecting member 100 is positioned on the specific cuvette 200 by the left and right moving unit 430, and the sample filling chamber 220 is positioned on the collecting member 100 by the front and rear moving unit 410. And when the collecting member 100 is inserted into the sample filling chamber 220 by the vertical movement unit 420 can provide a suction force to the collecting member 100 (see Fig. 16 (a)).
  • the collecting member 100 may be repeatedly provided with the suction force and the discharge force (see Fig. 16 (b)).
  • the detection unit 240 is positioned on the collecting member 100 by the front and rear moving unit 410 and the collecting member 100 is inserted into the detecting unit 240 by the vertical moving unit 420, the collecting member 100 is connected to the collecting member 100. Discharge power can be provided (see FIG. 16 (c)).
  • the pump unit 440 may include a conduit 441 and a pump 442, as shown in FIGS. 4 and 9.
  • the conduit 441 is formed through the arm 423 and transmits the pumping force of the pump 442 to the collecting member 100 through the arm 423.
  • the pump 442 is a conduit and a connection unit penetrating the inner portion of the arm. The pumping force is provided to the collecting member 100 through the conduit 441. Accordingly, since the pump unit 440 is provided, the amount of the sample, the reagent, or the reaction result may be precisely adjusted through the collecting member 100.
  • the second driving unit 600 includes a fourth connection bracket 602 to move the optical reader 500 and to which the optical reader 500 is connected; A second left and right guide part 604 for guiding the fourth connection bracket 602 in a left and right direction; And a second left and right driving unit 606 for applying a force in a left and right direction to the fourth connection bracket 602.
  • the left and right directions may be referred to as a direction in which the plurality of cuvets 200 are arranged side by side in a direction parallel to a direction in which the collecting member 100 moves by the first left and right driving unit 432 described above.
  • the second driving unit 600 positions the optical reader 500 on any one of the plurality of cuvets 200 arranged side by side in the left and right directions of the optical reader 500. You can.
  • the fourth connection bracket 602 is a device for fixing the optical reader 500 to be fixed and connected to the second left and right guide part 604 and the second left and right drive part 606. As shown in FIGS. 5A and 5B, the fourth connecting bracket 602 may be constituted by connecting means such as a leg extending to a predetermined length, for example.
  • the structure of the second left and right guides 604 is similar to that of the first left and right guides 431 described above. That is, the second left and right guide part 604 is provided in the second left and right guide rail 604a and the fourth connection bracket 602 which are provided to extend in the left and right direction in the housing 300 and the second It may include a second left and right guide portion 604b having a second left and right guide groove engaged with the left and right guide rails 604a.
  • the second left and right guide rails 604a extend in the left and right directions in the housing 300, and are provided in parallel with a path in which the fourth connection bracket 602 moves from side to side.
  • predetermined support members may be provided at both side ends of the second left and right guide rails 604a, respectively.
  • a member such as a predetermined bar extending between the support members may be provided to provide the second left and right guide rails 604a, and a second left and right guide rails 604a may be provided on the bars. .
  • the fourth connecting bracket 602 is provided with a second left and right guide part 604b.
  • the second left and right guide parts 604b include left and right guide grooves that engage with the second left and right guide rails 604a to allow the fourth connection bracket 602 to move along the second left and right guide rails 604a.
  • the second left and right drive unit 606 also has a configuration similar to the first left and right drive unit 432. That is, the second left and right driving unit 606 is provided at one side of the annular fourth belt 606a and the fourth belt 606a to which the fourth connection bracket 602 is fixed and extends in the left and right directions.
  • a fourth motor 606b for rotating the belt 606a and a fourth driven pulley 606c provided on the other side of the fourth belt 606a to rotatably support the fourth belt 606a may be configured. have.
  • the second left and right drive unit 606 applies a force in the left and right directions to the fourth connection bracket 602.
  • the fourth belt 606a and the fourth motor. 606b, and fourth driven pulley 606c For example, as illustrated in FIGS. 5A, 5B, and 10, the fourth belt 606a and the fourth motor. 606b, and fourth driven pulley 606c.
  • applying force in the left and right directions to the fourth connection bracket 602 means that the force in the left and right directions is applied to the vertical movement unit 420 and the collecting member 100, which are eventually connected to the fourth connection bracket 602. It can be taken to mean moving by adding.
  • the fourth belt 606a extends in the left and right direction and is fixed to the fourth connecting bracket 602 to transmit the power of the fourth motor 606b to the fourth connecting bracket 602.
  • the fourth motor 606b is provided at one side of the fourth belt 606a to rotate the fourth belt 606a.
  • the fourth driven pulley 606c is provided on the other side of the fourth belt 606a to rotatably support the fourth belt 606a.
  • the second left and right driving unit 606 of the pulley-belt type unlike the gear type, it is possible to prevent vibration and foreign substances due to friction generated when moving left and right can be made more accurate inspection.
  • the left and right shake can be prevented while being moved left and right to accurately move the optical reader 500.
  • a configuration in which the second left and right guide rails 604a and the second left and right guide parts 604b are connected to each other may be additionally provided to further prevent vibration.
  • the optical reader 500 is positioned on any one of the plurality of cuvettes 200 arranged side by side. The test can then be performed.
  • the station according to an embodiment of the present invention, the printed circuit board (PCB) (900, 910) in a position as shown in Figure 2b, which is provided inside the housing 300, for example And a control unit mounted on the printed circuit board and controlling the first driving unit 400 and the optical reader 500.
  • the control unit may include a process (see FIG. 15) in which the collecting member 100 in the waiting chamber 210 is coupled to the arm 423, and a cuvette () in the collecting member 100.
  • the sample filling chamber 220, the reagent filling chamber 230, and the detector 240 of the 200 are positioned so that the collecting member 100 is inserted into each chamber (see FIG. 16), and the collecting member 100
  • the first driving unit 400 is controlled in a process of separating from the arm 423 (see FIG. 18).
  • the second driving unit 600 may also be controlled.
  • FIG. 13 is a side view schematically illustrating a process in which the cuvette 200 is inserted into the holder 411 of the front and rear mobile unit.
  • the cuvet 200 is inserted through the inlet and outlet of the housing 300 and inserted into the holder 411 in the direction of the arrow, as shown in FIGS. 1 and 2, the cuvet 200 ) Is mounted to the holder 411.
  • the collecting member 100 is placed in the atmospheric chamber 210 of the cuvette 200, the sample is filled in the sample filling chamber 220, the reagent is filled in the reagent filling chamber 230, and The detector 240 is empty.
  • FIG. 14 and 15 are views illustrating a process in which the collecting member 100 seated on the cuvette 200 is coupled to the arm 423 of the vertical movement unit in a state in which the holder 411 of FIG. 13 is removed.
  • the holder 411 containing the cuvette 200 is moved by the front and rear movement unit 410.
  • the movement of the holder 411 which receives the cuvette 200 by the front and rear moving unit 410 is stopped,
  • the collecting member 100 is moved in the downward direction (see arrow) by the vertical movement unit 420.
  • the arm 423 is fitted to the collecting member 100 by the force of the lowering of the arm 423, the arm 423 is moved upward by the vertical movement unit 420. (See arrow 1), the holder 411 which accommodates the cuvette 200 is moved to the left side (see arrow 2) on the drawing by the forward and backward movement unit 410.
  • the sample filling chamber 220, the reagent filling chamber 230, and the detection unit 240 of the cuvette 200 are positioned in the collecting member 100 so that the collecting member 100 is disposed in each chamber. Describe the process of insertion.
  • FIG. 16 illustrates a sample filling chamber 220 and a reagent filling chamber of the cuvette 200 in the collecting member coupled to the arm 423 while the cuvette 200 of FIG. 15 is moved back and forth by the front and rear movement unit 410.
  • 230 is a diagram illustrating a process of locating the detector 230 and the detector 240.
  • the sample filling chamber 220 of the cuvette 200 moves to the left side in the drawing by the front and rear movement unit 410.
  • the collecting member 100 is moved downward in the drawing by the vertical movement unit 420 and inserted into the sample filling chamber 220.
  • the pump unit 440 is operated to suck the appropriate amount of the sample of the sample filling chamber 220 into the sampling member (100).
  • the reagent filling chamber 230 of the cuvette 200 is moved to the left side in the drawing by the cuvette 200 by the front and rear movement unit 410.
  • the sampling member 100 is moved downward in the drawing by the vertical movement unit 420 to be inserted into the reagent filling chamber 230.
  • the pump unit 440 is operated to react with the sample in the sample member 100 and the reagent in the reagent filling chamber 230 is mixed, the appropriate amount of the reaction result is sucked into the sample member (100).
  • the detection unit 240 of the cuvette 200 is the collecting member 100.
  • the collecting member 100 is moved downward in the drawing by the vertical movement unit 420 is inserted into the detection unit 240.
  • the pump unit 440 is operated to discharge the reaction product in the sampling member 100 to the detection unit 240.
  • the detection unit 240 when the reaction result is discharged to the detection unit 240, the cuvette 200 is moved to the right side in the drawing by the front and rear movement unit 410, the detection unit 240 is led to the optical reader 500 The reaction product of the detector 240 is analyzed by the optical reader 500.
  • 17 and 18 are views illustrating a process of separating the collecting member 100 from the arm 423.
  • the collecting member 100 is positioned on the removal unit 700 by the first horizontal movement unit 430.
  • the removal unit 700 is located on the left and right movement path of the collecting member (100).
  • the collecting member 100 moves in the downward direction by the vertical movement unit 420.
  • the collecting member 100 is inserted into the through hole 701, and then the collecting member inserted into the through hole 701 by sliding the slider 702 as shown by arrow 1. Pull 100 in one direction.
  • the collecting member 100 is separated from the arm 423 and falls down to be collected in the treatment box 708.
  • the collecting member 100 is made of a flexible material, the pulling by the through hole 701 and the separation of the other collecting member 100 can be easily achieved.
  • the removal unit 700 is provided with a spring, it can be restored to the original position after the slider 702 is operated.
  • the station according to the exemplary embodiment of the present invention may further include a display unit 830 provided in the housing 300 and displaying the analysis result by the optical reader 500. Therefore, the result of the analysis can be visually confirmed through the display unit 830, thereby enabling quick inspection.
  • the station according to the invention can be used for the simultaneous testing of a number of different analytes contained in biological samples.
  • the station according to an embodiment of the present invention as shown in Figure 1, is provided in the housing 300, the information of the sample filled in the sample filling chamber 220, the analyte to be included in the sample
  • the apparatus may further include one or more chips containing a recognition system including information regarding types and / or information about a specific driving method of the station, and a chip inserting unit 820 into which the chips are inserted.
  • the barcode is read by the barcode measuring unit or scanner 450 shown in FIG. 2B, and this information is used in conjunction with the information stored in the chip inserted into the chip inserting unit.
  • the chip and the chip insert are exemplarily controlled by a control unit mounted on a printed circuit board 930 substrate at a position as shown in FIG. 2B. Therefore, as compared with inputting the information of the sample through the keyboard, a faster and more accurate input is possible through the chip and the chip insertion unit 820. Therefore, the station can be driven to optimize the examination according to the various types of various analytes, so that various analytes can be easily inspected with one station, and the reproducibility and reliability of the test can be improved.
  • the station according to an embodiment of the present invention is provided in the housing 300.
  • the analysis result may further include a print output unit (not shown) which is printed and output. Therefore, even if a separate printer is not used, the test result can be provided directly through the print output unit as a document.
  • the station according to an embodiment of the present invention may further include a door in which a plurality of the cuvettes may enter and exit the station 300 in the housing 300. Therefore, during the inspection, the door may be closed to prevent foreign substances from entering the inside of the housing 300 so that more accurate inspection may be performed.
  • the above-described holder 411 may be further provided with a heater for applying heat to the holder 411 and a temperature sensor for sensing the temperature of the holder 411. Therefore, the sample contained in the sample filling chamber 220, the reagent contained in the reagent filling chamber 230, and the reaction product contained in the detection unit 240 may be maintained at an appropriate temperature required for inspection.
  • the holder 411 may have a predetermined sensor that can detect whether the collecting member 100 and the cuvette 200 and the mounting.
  • the sensor for detecting the mounting member will be described with reference to FIG. 7D.
  • An opening through which the collecting member can penetrate is provided on the lower surface of the collecting member standby chamber 210 into which the collecting member 100 of the cuvette 200 is inserted.
  • the opening is formed in the corresponding part also in a holder. Accordingly, the collecting member 100 passes through the cuvette and the holder, and the collecting member is sensed by the interrupter sensor 414 provided at the bottom when passing.
  • 7D shows the upper surface of the holder, and shows various shapes of the openings formed in the holder.
  • the upper figure allows the cuvet to be inserted into the holder first, and then the collecting member can be inserted. It shows a structure that can be inserted into the holder after inserting the collecting member first.
  • the above-mentioned sampling member detection sensor checks the start of the inspection of the presence or absence of the sampling member before the user starts the inspection, and checks whether the arm is inserted into the sampling member and driven upward.
  • Sensors 411e and 411f for detecting the cuvette mounting are provided at the rear of the holder as shown in FIG. 7E to confirm that the holder 411 having the cuvette necessary for the inspection is mounted on the equipment before the inspection starts. To improve convenience, accuracy and reliability.
  • the station according to an embodiment of the present invention is a front and rear movement unit cable chain 850, drive unit cable chain 860 and optical as shown by way of example in Figure 2a for the organization and management of the cable connected to the moving member Reader cable chain 870 may be further included.
  • the position of the cable chain can be variable.
  • the collecting member is put into the collecting member waiting chamber of the cuvette to recognize the collecting member and press the start button of the station. Accordingly, the cuvette moves backward, and then the barcode information is read by the barcode scanner, which is linked with the information of the ID chip to drive the station appropriate for the analyte. As a result, the arm is driven so that the sample is properly dispensed, and then the reaction between the sample and the reagent is performed for a predetermined temperature and time.
  • reaction product is dispensed into a detection unit including lateral flow chromatography, developed on a chromatography membrane, and then detected by the fluorescence signal, and the information is used to determine a specific analyte included in the sample.
  • a detection unit including lateral flow chromatography, developed on a chromatography membrane, and then detected by the fluorescence signal, and the information is used to determine a specific analyte included in the sample.
  • Each step is shown through a display provided in the station, and when the analysis is finished, the collecting member does not contain the collecting member in the waiting chamber of the collecting member, and the cuvette is mounted on the holder. The analysis ends.
  • 22 to 25 are diagrams showing a system configuration of a station according to the present invention.
  • FIG. 22 is a control block diagram of the main system.
  • the main system is configured to operate with the central processor unit (CPU), microprocessor, USB, RS232 interface, motor control unit, system memory, heating system, and input / output unit.
  • the central processing unit (CPU) drives the operating system, and may exchange commands with the system memory, input / output devices, RS232, and USB interfaces.
  • the microprocessor may be included in the controller, and may drive and control the motor controller, the temperature controller, and the like.
  • the input / output device may include an input device such as a keyboard, a mouse, a touch pad, and an output device such as a monitor or a printer.
  • the operating system may include applications required for driving various equipment and information input and output, and includes a separate controller for data input and output (screen output, printer, data storage, etc.).
  • the operating system may drive and control devices through a device driver, and may also be driven and controlled using a controller including a microprocessor.
  • a separate controller may be provided for independent driving and control of the driving unit of each cartridge, and control such as heater control and position initialization may be directly driven and controlled through an operating system.
  • a temperature sensor is attached to the heater block, and the signal of the temperature sensor is processed by the processor through an amplifier. At this time, the set temperature can be controlled by the feedback circuit.
  • the heater control circuit consists of a temperature sensor and an analog / digital signal converter, a processor, a digital / analog converter, a shock absorber and a heater.
  • the analog signal from the temperature sensor is converted into a digital signal through a converter, and is configured in a form that can be controlled to a set temperature by starting or stopping the heater by a driving driver.
  • waiting chamber 220 sample filling chamber
  • 411a mounting channel 411b: holder cuvette fixing member
  • 412b Front and rear guide rails 412c: Front and rear guide grooves
  • front and rear drive unit 413a first connection bracket
  • Second driven pulley 430 First left and right moving unit
  • First left and right guide portion 431a First left and right guide rails
  • connection bracket 604 second left and right guide portion
  • 604a second left and right guide rails 604b: second left and right guide parts
  • 606 second left and right drive unit 606a: fourth belt
  • 606b fourth motor 606c: fourth driven pulley
  • treatment box 820 chip insert
  • display unit 850 front and rear movement unit cable chain

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Hydrology & Water Resources (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

La présente invention a pour objet technique de fournir un poste, permettant de tester un analyte dans un échantillon, ce qui permet une réaction et une analyse rapides et précises de l'échantillon et d'un réactif dans un seul appareil. À cette fin, la présente invention concerne un poste, qui est destiné à tester un échantillon par insertion d'une cuvette, ayant une chambre d'attente sur laquelle est placé un élément de collecte, une chambre de remplissage d'échantillon, une chambre de remplissage de réactif et une unité de détection. Le poste comprend : un boîtier qui comporte un orifice d'entrée/de sortie dans lequel est insérée une cuvette ; une unité d'entraînement qui est disposée à l'intérieur du boîtier, déplace horizontalement la cuvette, déplace verticalement un élément de collecte, fait réagir un échantillon dans une chambre de remplissage d'échantillon et un réactif dans une chambre de remplissage de réactif, et injecte un résultat de réaction de celle-ci dans une unité de détection ; et un lecteur optique qui est disposé sur le trajet de déplacement horizontal de la cuvette et est destiné à analyser le résultat de réaction.
PCT/KR2016/005050 2015-05-14 2016-05-13 Poste, utilisé pour un appareil de test, ayant un moyen de réaction et de détection intégré WO2016182382A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
ES16793028T ES2826865T3 (es) 2015-05-14 2016-05-13 Estación destinada al uso con un aparato de ensayo que disponga de medios de reacción y detección integrados
EP16793028.8A EP3296718B1 (fr) 2015-05-14 2016-05-13 Station à utiliser avec un appareil d'essai ayant des moyens de réaction et de détection intégrés
CN201680039919.9A CN107923839B (zh) 2015-05-14 2016-05-13 用于测试装置、具有集成的反应和检测机构的站
US15/574,131 US10670499B2 (en) 2015-05-14 2016-05-13 Station, used for test apparatus, having integrated reaction and detection means
US15/817,089 US10871474B2 (en) 2015-05-14 2017-11-17 System and method for analyzing biological fluid in multiple cuvettes

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2015-0067278 2015-05-14
KR20150067278 2015-05-14
KR1020160058165A KR101811786B1 (ko) 2015-05-14 2016-05-12 일체화된 반응 및 검출 수단을 구비한 시험 장치에 사용되는 스테이션
KR10-2016-0058165 2016-05-12

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US15/574,131 A-371-Of-International US10670499B2 (en) 2015-05-14 2016-05-13 Station, used for test apparatus, having integrated reaction and detection means
US15/817,089 Continuation-In-Part US10871474B2 (en) 2015-05-14 2017-11-17 System and method for analyzing biological fluid in multiple cuvettes

Publications (1)

Publication Number Publication Date
WO2016182382A1 true WO2016182382A1 (fr) 2016-11-17

Family

ID=57248141

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2016/005050 WO2016182382A1 (fr) 2015-05-14 2016-05-13 Poste, utilisé pour un appareil de test, ayant un moyen de réaction et de détection intégré

Country Status (1)

Country Link
WO (1) WO2016182382A1 (fr)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108254371A (zh) * 2017-12-26 2018-07-06 深圳德夏生物医学工程有限公司 便携式检测分析仪
CN109521210A (zh) * 2017-09-19 2019-03-26 江苏雷镈智能科技有限公司 全自动试管装载设备及其方法
CN109738256A (zh) * 2019-02-27 2019-05-10 苏州雷奈威科技有限公司 一种食品检测预处理装置及预处理方法
CN110220895A (zh) * 2019-06-28 2019-09-10 中国计量大学 一种高通量便捷式胶体金ccd读数仪的图像采集装置
CN112858618A (zh) * 2021-03-03 2021-05-28 南阳理工学院 一种环境监测用污水化学分析工具
CN114646769A (zh) * 2022-03-18 2022-06-21 广东凯金新能源科技股份有限公司 一种用于石墨振实检测的自动化检测系统及其使用方法
CN117405673A (zh) * 2023-10-31 2024-01-16 山东宽福生物科技有限公司 一种食品成分检测装置

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100007720A (ko) * 2008-07-10 2010-01-22 삼성전자주식회사 시약 카트리지, 시약 카트리지를 구비하는 미세유동장치, 그 제조방법, 및 이를 이용한 시료분석방법
KR20100027389A (ko) * 2008-09-02 2010-03-11 삼성전자주식회사 미세유동장치 및 미세유동장치에의 시료주입방법
KR20120025580A (ko) * 2009-06-04 2012-03-15 유니바사루 바이오 리사치 가부시키가이샤 검체 검사장치 및 그 방법
KR20130072987A (ko) * 2011-12-22 2013-07-02 (주)케이엔알시스템 검사 방법
US20140023568A1 (en) * 2012-07-19 2014-01-23 Electronics And Telecommunications Research Institute Bio cartridges

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100007720A (ko) * 2008-07-10 2010-01-22 삼성전자주식회사 시약 카트리지, 시약 카트리지를 구비하는 미세유동장치, 그 제조방법, 및 이를 이용한 시료분석방법
KR20100027389A (ko) * 2008-09-02 2010-03-11 삼성전자주식회사 미세유동장치 및 미세유동장치에의 시료주입방법
KR20120025580A (ko) * 2009-06-04 2012-03-15 유니바사루 바이오 리사치 가부시키가이샤 검체 검사장치 및 그 방법
KR20130072987A (ko) * 2011-12-22 2013-07-02 (주)케이엔알시스템 검사 방법
US20140023568A1 (en) * 2012-07-19 2014-01-23 Electronics And Telecommunications Research Institute Bio cartridges

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109521210A (zh) * 2017-09-19 2019-03-26 江苏雷镈智能科技有限公司 全自动试管装载设备及其方法
CN108254371A (zh) * 2017-12-26 2018-07-06 深圳德夏生物医学工程有限公司 便携式检测分析仪
CN108254371B (zh) * 2017-12-26 2024-02-09 深圳德夏生物医学工程有限公司 便携式检测分析仪
CN109738256A (zh) * 2019-02-27 2019-05-10 苏州雷奈威科技有限公司 一种食品检测预处理装置及预处理方法
CN109738256B (zh) * 2019-02-27 2024-03-08 苏州鑫点石精密机械制造有限公司 一种食品检测预处理装置及预处理方法
CN110220895A (zh) * 2019-06-28 2019-09-10 中国计量大学 一种高通量便捷式胶体金ccd读数仪的图像采集装置
CN112858618A (zh) * 2021-03-03 2021-05-28 南阳理工学院 一种环境监测用污水化学分析工具
CN114646769A (zh) * 2022-03-18 2022-06-21 广东凯金新能源科技股份有限公司 一种用于石墨振实检测的自动化检测系统及其使用方法
CN117405673A (zh) * 2023-10-31 2024-01-16 山东宽福生物科技有限公司 一种食品成分检测装置
CN117405673B (zh) * 2023-10-31 2024-05-03 山东宽福生物科技有限公司 一种食品成分检测装置

Similar Documents

Publication Publication Date Title
WO2016182382A1 (fr) Poste, utilisé pour un appareil de test, ayant un moyen de réaction et de détection intégré
WO2018143680A1 (fr) Appareil d'immunoessai en phase liquide automatisé
KR101811786B1 (ko) 일체화된 반응 및 검출 수단을 구비한 시험 장치에 사용되는 스테이션
JP6871345B2 (ja) 分析評価装置、方法、および試薬
WO2020027470A1 (fr) Appareil d'immunoessai en phase liquide automatisé et procédé associé
JP4890699B2 (ja) 試料品質測定を行う分析装置および方法
KR101646549B1 (ko) 일체화된 반응 및 검출 수단을 구비한 시험 장치용 시스템
US10871474B2 (en) System and method for analyzing biological fluid in multiple cuvettes
WO2013041006A1 (fr) Analyseur biochimique
US7872749B2 (en) Instrument for making optical measurements on multiple samples retained by surface tension
WO2016088992A1 (fr) Appareil de test et son procédé de commande
KR102568429B1 (ko) 자동 체외진단 검사장치
WO2021177548A1 (fr) Kit de dosage immunologique à écoulement latéral entièrement automatique et dispositif de mesure pour la mesure de multiples biomarqueurs de la salive
JP7129533B2 (ja) 試料処理装置及び試料吸引方法
WO2012144859A2 (fr) Procédé et appareil pour l'analyse d'interaction protéine-protéine au niveau d'une molécule unique dans un environnement cellulaire
WO2015072757A1 (fr) Station destinée à un dispositif d'essai équipée d'un moyen de réaction et de détection intégré
US20200225158A1 (en) Analyzer
WO2022182125A1 (fr) Cartouche de chargement de gouttelettes de type désactivé intégrée à un dispositif de commande de chaleur pour mesurer un signal optique
WO2022075676A1 (fr) Appareil d'essai d'amplification d'acide nucléique, et système d'analyse d'échantillon automatique le comprenant
WO2023074988A1 (fr) Procédé d'analyse de réponse immunitaire liquide à l'aide de billes magnétiques
WO2020134630A1 (fr) Analyseur elisa
WO2020235964A1 (fr) Appareil de diagnostic pour immunoessai
WO2023172060A1 (fr) Dispositif de test d'échantillon et procédé de test d'échantillon
WO2022080994A1 (fr) Trousse de test de diagnostic covid
JP2014228424A (ja) 検査用パッケージ及びその検査方法、スクリーニング方法並びにスクリーニング装置

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 16793028

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 15574131

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2016793028

Country of ref document: EP