WO2017124590A1 - Preparation method and application of water-soluble natural nitric oxide - Google Patents
Preparation method and application of water-soluble natural nitric oxide Download PDFInfo
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- WO2017124590A1 WO2017124590A1 PCT/CN2016/073522 CN2016073522W WO2017124590A1 WO 2017124590 A1 WO2017124590 A1 WO 2017124590A1 CN 2016073522 W CN2016073522 W CN 2016073522W WO 2017124590 A1 WO2017124590 A1 WO 2017124590A1
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- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 title claims abstract description 64
- 238000002360 preparation method Methods 0.000 title 1
- 238000000855 fermentation Methods 0.000 claims abstract description 41
- 230000004151 fermentation Effects 0.000 claims abstract description 41
- 230000009286 beneficial effect Effects 0.000 claims abstract description 39
- 241000894006 Bacteria Species 0.000 claims abstract description 37
- 239000002994 raw material Substances 0.000 claims abstract description 23
- 238000000034 method Methods 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000011081 inoculation Methods 0.000 claims abstract description 8
- 239000004475 Arginine Substances 0.000 claims abstract description 7
- 102000004190 Enzymes Human genes 0.000 claims abstract description 7
- 108090000790 Enzymes Proteins 0.000 claims abstract description 7
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims abstract description 7
- 108010064851 Plant Proteins Proteins 0.000 claims abstract description 6
- 235000021118 plant-derived protein Nutrition 0.000 claims abstract description 6
- 210000004204 blood vessel Anatomy 0.000 claims abstract description 5
- 201000010099 disease Diseases 0.000 claims abstract description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 3
- 241000196324 Embryophyta Species 0.000 claims description 12
- 238000004519 manufacturing process Methods 0.000 claims description 7
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 6
- 244000063299 Bacillus subtilis Species 0.000 claims description 3
- 108010082495 Dietary Plant Proteins Proteins 0.000 claims description 3
- 235000009811 Momordica charantia Nutrition 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 244000078912 Trichosanthes cucumerina Species 0.000 claims description 3
- 235000008322 Trichosanthes cucumerina Nutrition 0.000 claims description 3
- 235000014655 lactic acid Nutrition 0.000 claims description 3
- 239000004310 lactic acid Substances 0.000 claims description 3
- 235000008658 Artemisia capillaris Nutrition 0.000 claims description 2
- 241000092668 Artemisia capillaris Species 0.000 claims description 2
- 244000068988 Glycine max Species 0.000 claims description 2
- 235000010469 Glycine max Nutrition 0.000 claims description 2
- 230000035622 drinking Effects 0.000 claims description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 claims 1
- 206010020772 Hypertension Diseases 0.000 abstract description 3
- 206010008118 cerebral infarction Diseases 0.000 abstract description 3
- 208000026106 cerebrovascular disease Diseases 0.000 abstract description 3
- 206010012601 diabetes mellitus Diseases 0.000 abstract description 3
- 238000005406 washing Methods 0.000 abstract description 2
- 235000013361 beverage Nutrition 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 229940079593 drug Drugs 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 240000002234 Allium sativum Species 0.000 description 1
- 108010073771 Soybean Proteins Proteins 0.000 description 1
- 235000004611 garlic Nutrition 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 235000019710 soybean protein Nutrition 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
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- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Definitions
- the invention relates to the technical field of plant enzymes, in particular to a method and application for producing water-soluble natural nitric oxide.
- the current plant fermentation technology can ferment a part of the raw materials, but can not produce nitric oxide and use the current scientific technology to artificially inject human nitric oxide into beverages or foods and medicines, and the production maintains beneficial nitric oxide.
- the technology of the products has not yet been developed, and the raw materials used are all collected from ordinary plants and can be used to produce popular products at low cost. Therefore, the present invention proposes a method and application for producing water-soluble natural nitric oxide.
- the object of the present invention is to solve the shortcomings of the prior art, and to provide a method and application for producing water-soluble natural nitric oxide, which is simple in process and low in cost.
- a method for preparing water-soluble natural nitric oxide comprising the following steps;
- cultivating complex beneficial bacteria selecting a variety of beneficial bacteria for plant fermentation, and mixing the beneficial bacteria according to the preset proportion;
- inoculation fermentation the complex beneficial bacteria in S2 are inoculated into the raw materials of S1, and water is added; after 28-30 ° C constant temperature fermentation, a large amount of water-soluble nitric oxide is obtained.
- the raw material of the vegetable protein in S1 may be selected from Artemisia capillaris, bitter gourd and soybean.
- the beneficial bacteria fermented by the plant in S2 are selected from the group consisting of lactic acid bacteria Bacillus subtilis and yeast.
- the watering depth in S3 is 50 times the height of the raw material stacking and is energized into the water.
- a water-soluble natural nitric oxide produced by a fermentation broth that is rich in enzymes and can improve the associated blood vessels when consumed.
- the invention produces a method for producing nitric oxide by plant fermentation of beneficial bacteria and related product production; the beneficial enzyme and nitric oxide produced by the fermentation of the invention can produce beverages, foods and medicines beneficial to human health.
- the invention has simple process and low cost, and has obvious difference compared with a little fermentation process. First, it is very important to select raw materials, and secondly, inoculate special beneficial bacteria which have been developed and retained, and produce and preserve a large number of fermentation processes by preset time. Water-soluble natural nitric oxide, fermented fermentation broth is rich in enzymes, can improve the associated blood vessels, such as high blood pressure, diabetes, cerebral infarction.
- FIG. 1 is a schematic structural view of a method for producing water-soluble natural nitric oxide according to the present invention.
- S1 material selection: selecting the plant protein containing a large amount of arginine, and washing it, smashing it, and placing it in the fermentation chamber for use;
- cultivating complex beneficial bacteria selecting a variety of beneficial bacteria for plant fermentation, and mixing the beneficial bacteria according to the preset proportion;
- a method for preparing water-soluble natural nitric oxide according to the embodiment includes the following steps;
- cultivating complex beneficial bacteria selecting a variety of beneficial bacteria for plant fermentation, and mixing the beneficial bacteria according to the preset proportion;
- inoculation fermentation inoculate the complex beneficial bacteria in S2 onto the raw materials in S1, and add water; after 28-30 ° C constant temperature fermentation, a large amount of water-soluble nitric oxide is obtained, in addition to producing a beverage beneficial to human health. , food and medicine, also have the effect of tonifying the kidney.
- a method for preparing water-soluble natural nitric oxide according to the embodiment includes the following steps;
- cultivating complex beneficial bacteria selecting a variety of beneficial bacteria for plant fermentation, and mixing the beneficial bacteria according to the preset proportion;
- inoculation fermentation inoculate the complex beneficial bacteria in S2 onto the raw materials in S1, and add water; after 28-30 ° C constant temperature fermentation, a large amount of water-soluble nitric oxide is obtained, in addition to producing a beverage beneficial to human health. , food and medicine, also have the effect of fire.
- a method for preparing water-soluble natural nitric oxide according to the embodiment includes the following steps;
- cultivating complex beneficial bacteria selecting a variety of beneficial bacteria for plant fermentation, such as lactic acid bacteria Bacillus subtilis, yeast, according to a predetermined proportion of the amount of bacteria, cultured complex bacteria;
- inoculation fermentation the complex beneficial bacteria in S2 are inoculated into the raw materials of S1, and water is added; after 28-30 ° C constant temperature fermentation, a large amount of water-soluble nitric oxide is obtained.
- the invention also proposes an application of water-soluble natural nitric oxide, which is rich in enzymes, and can improve diseases associated with blood vessels, such as hypertension, diabetes, cerebral infarction, etc. when drinking.
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Abstract
Disclosed is a method for preparing a water-soluble natural nitric oxide. The method comprises the following steps: S1, selection of raw materials: selecting raw materials comprising plant proteins having large quantities of arginine, washing the raw materials, comminuting the cleaned raw materials, and storing the comminuted raw materials in a fermentation room for later use; S2, cultivation of a beneficial bacteria complex: selecting a plurality of beneficial bacteria for plant fermentation, mixing the beneficial bacteria at a predetermined ratio, and cultivating the beneficial bacteria to obtain the beneficial bacteria complex; and S3, inoculation and fermentation: inoculating the beneficial bacteria complex obtained in S2 into the raw materials obtained in S1 and adding water, and allowing fermentation at constant temperatures of 28-30˚C to obtain a large quantity of the water-soluble nitric oxide. The method significantly differs from a fermentation process of the prior art. Firstly, the selection of raw materials is very important. Secondly, inoculation is performed using particular beneficial bacteria developed and maintained by the applicant, and fermentation is conducted for a predetermined length of time to produce and store a large quantity of the water-soluble natural nitric oxide. A fermentation broth produced by the fermentation comprises abundant enzymes, and can be consumed to ameliorate a blood vessel-related disease such as hypertension, diabetes, cerebral infarction, and the like.
Description
本发明涉及植物酵素技术领域，尤其涉及一种水溶性天然一氧化氮的制作方法及应用。The invention relates to the technical field of plant enzymes, in particular to a method and application for producing water-soluble natural nitric oxide.
目前的植物发酵技术能发酵一部分的原材料，但生产不出一氧化氮并且用现在的科学技术把对人体有益的一氧化氮人为的注入到饮料或食品及药品内，且生产保持有益一氧化氮的产品的技术还没开发，况且所用的原材料都是从一般的植物中收取可以用低成本生产出大众化的产品。因此本发明提出了一种水溶性天然一氧化氮的制作方法及应用。The current plant fermentation technology can ferment a part of the raw materials, but can not produce nitric oxide and use the current scientific technology to artificially inject human nitric oxide into beverages or foods and medicines, and the production maintains beneficial nitric oxide. The technology of the products has not yet been developed, and the raw materials used are all collected from ordinary plants and can be used to produce popular products at low cost. Therefore, the present invention proposes a method and application for producing water-soluble natural nitric oxide.
发明内容Summary of the invention
本发明的目的是为了解决现有技术中存在的缺点，而提出的一种水溶性天然一氧化氮的制作方法及应用，工艺简单，成本低。The object of the present invention is to solve the shortcomings of the prior art, and to provide a method and application for producing water-soluble natural nitric oxide, which is simple in process and low in cost.
为了实现上述目的，本发明采用了如下技术方案：In order to achieve the above object, the present invention adopts the following technical solutions:
一种水溶性天然一氧化氮的制作方法，包括如下步骤；A method for preparing water-soluble natural nitric oxide, comprising the following steps;
S1、选材：选取含有大量精氨酸的植物性蛋白质的原材料，清洗干净后并粉碎，放置在发酵室内备用；S1. Selection: Raw materials of plant protein containing a large amount of arginine are selected, cleaned and pulverized, and placed in a fermentation chamber for use;
S2、培养复合有益菌：选取多种植物发酵的有益菌，按预设比例量配合，培养出复合有益菌；S2, cultivating complex beneficial bacteria: selecting a variety of beneficial bacteria for plant fermentation, and mixing the beneficial bacteria according to the preset proportion;
S3、接种发酵：将S2中的复合有益菌接种到S1中原材料上，并加水；28—30℃恒温发酵后，即得大量的水溶性一氧化氮。
S3, inoculation fermentation: the complex beneficial bacteria in S2 are inoculated into the raw materials of S1, and water is added; after 28-30 ° C constant temperature fermentation, a large amount of water-soluble nitric oxide is obtained.
优选的，S1中的植物性蛋白质的原材料可选用茵陈蒿、苦瓜和黄豆。Preferably, the raw material of the vegetable protein in S1 may be selected from Artemisia capillaris, bitter gourd and soybean.
优选的，S2中植物发酵的有益菌选用乳酸菌枯草芽孢杆菌、酵母菌。Preferably, the beneficial bacteria fermented by the plant in S2 are selected from the group consisting of lactic acid bacteria Bacillus subtilis and yeast.
优选的，S3中的加水深度为原料堆放高度的50倍，并向水中通电。Preferably, the watering depth in S3 is 50 times the height of the raw material stacking and is energized into the water.
一种水溶性天然一氧化氮的应用，产生的发酵液是含有丰富的酵素，饮用时能改善关联血管的疾病。A water-soluble natural nitric oxide produced by a fermentation broth that is rich in enzymes and can improve the associated blood vessels when consumed.
本发明通过有益菌的植物发酵，制造一氧化氮的方法及其有关产品生产；本发明发酵产生的有益酵素及一氧化氮，能生产出对人体健康有益的饮料、食品和药物。本发明工艺简单，成本低，比已既有点发酵工艺有明显的差别，首先挑选原材料很重要，其次是把已开发保留的特殊有益菌接种，通过在预设时间的发酵过程生产及保存大量的水溶性天然一氧化氮，发酵产生的发酵液是含有丰富的酵素，饮用时能改善关联血管的疾病，如高血压、糖尿病、脑梗塞等。The invention produces a method for producing nitric oxide by plant fermentation of beneficial bacteria and related product production; the beneficial enzyme and nitric oxide produced by the fermentation of the invention can produce beverages, foods and medicines beneficial to human health. The invention has simple process and low cost, and has obvious difference compared with a little fermentation process. First, it is very important to select raw materials, and secondly, inoculate special beneficial bacteria which have been developed and retained, and produce and preserve a large number of fermentation processes by preset time. Water-soluble natural nitric oxide, fermented fermentation broth is rich in enzymes, can improve the associated blood vessels, such as high blood pressure, diabetes, cerebral infarction.
图1为本发明提出的一种水溶性天然一氧化氮的制作方法的结构示意图。1 is a schematic structural view of a method for producing water-soluble natural nitric oxide according to the present invention.
下面结合具体实施例来对本发明做进一步解释。The invention is further explained below in conjunction with specific embodiments.
实施例一Embodiment 1
参照图1，本实施例提出的一种水溶性天然一氧化氮的制作方法，
包括如下步骤；Referring to Figure 1, a method for preparing water-soluble natural nitric oxide according to the present embodiment,
Including the following steps;
S1、选材：选取含有大量精氨酸的植物性蛋白质的茵陈蒿，清洗干净后并粉碎，放置在发酵室内备用；S1, material selection: selecting the plant protein containing a large amount of arginine, and washing it, smashing it, and placing it in the fermentation chamber for use;
S2、培养复合有益菌：选取多种植物发酵的有益菌，按预设比例量配合，培养出复合有益菌；S2, cultivating complex beneficial bacteria: selecting a variety of beneficial bacteria for plant fermentation, and mixing the beneficial bacteria according to the preset proportion;
S3、接种发酵：将S2中的复合有益菌接种到S1中原材料上，并加水；28—30℃恒温发酵后，即得大量含有蒜香的水溶性一氧化氮。S3, inoculation fermentation: the complex beneficial bacteria in S2 are inoculated into the raw materials of S1, and water is added; after 28-30 ° C constant temperature fermentation, a large amount of water-soluble nitric oxide containing garlic is obtained.
实施例二Embodiment 2
参照图1，本实施例提出的一种水溶性天然一氧化氮的制作方法，包括如下步骤；Referring to FIG. 1 , a method for preparing water-soluble natural nitric oxide according to the embodiment includes the following steps;
S1、选材：选取含有大量精氨酸的植物性蛋白质的黄豆，清洗干净后并粉碎，放置在发酵室内备用；S1. Selection: Select the soybean protein containing a large amount of arginine vegetable protein, clean it and pulverize it, and place it in the fermentation chamber for use;
S2、培养复合有益菌：选取多种植物发酵的有益菌，按预设比例量配合，培养出复合有益菌；S2, cultivating complex beneficial bacteria: selecting a variety of beneficial bacteria for plant fermentation, and mixing the beneficial bacteria according to the preset proportion;
S3、接种发酵：将S2中的复合有益菌接种到S1中原材料上，并加水；28—30℃恒温发酵后，即得大量的水溶性一氧化氮，除了能生产出对人体健康有益的饮料、食品和药物，还具有补肾的功效。S3, inoculation fermentation: inoculate the complex beneficial bacteria in S2 onto the raw materials in S1, and add water; after 28-30 ° C constant temperature fermentation, a large amount of water-soluble nitric oxide is obtained, in addition to producing a beverage beneficial to human health. , food and medicine, also have the effect of tonifying the kidney.
实施例三Embodiment 3
参照图1，本实施例提出的一种水溶性天然一氧化氮的制作方法，包括如下步骤；Referring to FIG. 1 , a method for preparing water-soluble natural nitric oxide according to the embodiment includes the following steps;
S1、选材：选取含有大量精氨酸的植物性蛋白质的苦瓜，清洗干净后并粉碎，放置在发酵室内备用；
S1. Selection: The bitter gourd containing the plant protein containing a large amount of arginine is cleaned and pulverized, and placed in the fermentation chamber for use;
S2、培养复合有益菌：选取多种植物发酵的有益菌，按预设比例量配合，培养出复合有益菌；S2, cultivating complex beneficial bacteria: selecting a variety of beneficial bacteria for plant fermentation, and mixing the beneficial bacteria according to the preset proportion;
S3、接种发酵：将S2中的复合有益菌接种到S1中原材料上，并加水；28—30℃恒温发酵后，即得大量的水溶性一氧化氮，除了能生产出对人体健康有益的饮料、食品和药物，还具有去火的功效。S3, inoculation fermentation: inoculate the complex beneficial bacteria in S2 onto the raw materials in S1, and add water; after 28-30 ° C constant temperature fermentation, a large amount of water-soluble nitric oxide is obtained, in addition to producing a beverage beneficial to human health. , food and medicine, also have the effect of fire.
实施例四Embodiment 4
参照图1，本实施例提出的一种水溶性天然一氧化氮的制作方法，包括如下步骤；Referring to FIG. 1 , a method for preparing water-soluble natural nitric oxide according to the embodiment includes the following steps;
S1、选材：选取含有大量精氨酸的植物性蛋白质的原材料，清洗干净后并粉碎，放置在发酵室内备用；S1. Selection: Raw materials of plant protein containing a large amount of arginine are selected, cleaned and pulverized, and placed in a fermentation chamber for use;
S2、培养复合有益菌：选取多种植物发酵的有益菌，如乳酸菌枯草芽孢杆菌、酵母菌，按预设比例量配合，培养出复合有益菌；S2, cultivating complex beneficial bacteria: selecting a variety of beneficial bacteria for plant fermentation, such as lactic acid bacteria Bacillus subtilis, yeast, according to a predetermined proportion of the amount of bacteria, cultured complex bacteria;
S3、接种发酵：将S2中的复合有益菌接种到S1中原材料上，并加水；28—30℃恒温发酵后，即得大量的水溶性一氧化氮。S3, inoculation fermentation: the complex beneficial bacteria in S2 are inoculated into the raw materials of S1, and water is added; after 28-30 ° C constant temperature fermentation, a large amount of water-soluble nitric oxide is obtained.
本发明还提出了一种水溶性天然一氧化氮的应用，产生的发酵液是含有丰富的酵素，饮用时能改善关联血管的疾病，如高血压、糖尿病、脑梗塞等。The invention also proposes an application of water-soluble natural nitric oxide, which is rich in enzymes, and can improve diseases associated with blood vessels, such as hypertension, diabetes, cerebral infarction, etc. when drinking.
以上所述，仅为本发明较佳的具体实施方式，但本发明的保护范围并不局限于此，任何熟悉本技术领域的技术人员在本发明揭露的技术范围内，根据本发明的技术方案及其发明构思加以等同替换或改变，都应涵盖在本发明的保护范围之内。
The above is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any technical person skilled in the art within the technical scope disclosed by the present invention, the technical solution according to the present invention Equivalent substitutions or modifications of the inventive concept are intended to be included within the scope of the invention.
Claims (5)
- 一种水溶性天然一氧化氮的制作方法，其特征在于，包括如下步骤；A method for preparing water-soluble natural nitric oxide, comprising the following steps;S1、选材：选取含有大量精氨酸的植物性蛋白质的原材料，清洗干净后并粉碎，放置在发酵室内备用；S1. Selection: Raw materials of plant protein containing a large amount of arginine are selected, cleaned and pulverized, and placed in a fermentation chamber for use;S2、培养复合有益菌：选取多种植物发酵的有益菌，按预设比例量配合，培养出复合有益菌；S2, cultivating complex beneficial bacteria: selecting a variety of beneficial bacteria for plant fermentation, and mixing the beneficial bacteria according to the preset proportion;S3、接种发酵：将S2中的复合有益菌接种到S1中原材料上，并加水；28—30℃恒温发酵后，即得大量的水溶性一氧化氮。S3, inoculation fermentation: the complex beneficial bacteria in S2 are inoculated into the raw materials of S1, and water is added; after 28-30 ° C constant temperature fermentation, a large amount of water-soluble nitric oxide is obtained.
- 根据权利要求1所述的一种水溶性天然一氧化氮的制作方法，其特征在于，S1中的植物性蛋白质的原材料可选用茵陈蒿、苦瓜和黄豆。The method for producing water-soluble natural nitric oxide according to claim 1, wherein the raw material of the vegetable protein in S1 is selected from the group consisting of Artemisia capillaris, bitter gourd and soybean.
- 根据权利要求1所述的一种水溶性天然一氧化氮的制作方法，其特征在于，S2中植物发酵的有益菌选用乳酸菌枯草芽孢杆菌、酵母菌。The method for producing water-soluble natural nitric oxide according to claim 1, wherein the beneficial bacteria for plant fermentation in S2 are lactic acid bacteria such as Bacillus subtilis and yeast.
- 根据权利要求1所述的一种水溶性天然一氧化氮的制作方法，其特征在于，S3中的加水深度为原料堆放高度的50倍，并向水中通电。The method for producing water-soluble natural nitric oxide according to claim 1, wherein the water addition depth in S3 is 50 times the height of the raw material stacking, and is energized into the water.
- 一种水溶性天然一氧化氮的应用，其特征在于，产生的发酵液是含有丰富的酵素，饮用时能改善关联血管的疾病。 The use of a water-soluble natural nitric oxide is characterized in that the produced fermentation broth is rich in enzymes and can improve diseases associated with blood vessels when drinking.
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