WO2017117113A8 - Single molecule counting for analysis from dried blood spots - Google Patents

Single molecule counting for analysis from dried blood spots Download PDF

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Publication number
WO2017117113A8
WO2017117113A8 PCT/US2016/068685 US2016068685W WO2017117113A8 WO 2017117113 A8 WO2017117113 A8 WO 2017117113A8 US 2016068685 W US2016068685 W US 2016068685W WO 2017117113 A8 WO2017117113 A8 WO 2017117113A8
Authority
WO
WIPO (PCT)
Prior art keywords
blood
analyte
complex
analysis
sample
Prior art date
Application number
PCT/US2016/068685
Other languages
French (fr)
Other versions
WO2017117113A1 (en
Inventor
Jeffrey Bishop
Ali Mukherjee
Tam DANG
Heather MORRELL
Original Assignee
Singulex, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Singulex, Inc. filed Critical Singulex, Inc.
Publication of WO2017117113A1 publication Critical patent/WO2017117113A1/en
Publication of WO2017117113A8 publication Critical patent/WO2017117113A8/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates

Abstract

The disclosure is directed to the ultra-sensitive detection of analytes obtained from blood samples collected on blood collection cards. Disclosed herein is a method for analyzing a blood sample for the presence or amount of an analyte. The method includes extracting dried blood representing a whole blood sample volume from a blood collection card; forming a complex between analyte in the extracted sample and a labeled binding partner for the analyte comprising a fluorescent moiety; and determining the amount of binding partner from the complex by analyzing a processing sample comprising the fluorescent moiety from the complex with an analyzer capable of the ultra-sensitive detection of the moiety.
PCT/US2016/068685 2015-12-30 2016-12-27 Single molecule counting for analysis from dried blood spots WO2017117113A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562273400P 2015-12-30 2015-12-30
US62/273,400 2015-12-30

Publications (2)

Publication Number Publication Date
WO2017117113A1 WO2017117113A1 (en) 2017-07-06
WO2017117113A8 true WO2017117113A8 (en) 2018-02-01

Family

ID=59225749

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2016/068685 WO2017117113A1 (en) 2015-12-30 2016-12-27 Single molecule counting for analysis from dried blood spots

Country Status (1)

Country Link
WO (1) WO2017117113A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021202893A1 (en) * 2020-04-03 2021-10-07 Nonigenex, Inc. Detecting adaptive immunity to coronavirus

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8685711B2 (en) * 2004-09-28 2014-04-01 Singulex, Inc. Methods and compositions for highly sensitive detection of molecules
US9040305B2 (en) * 2004-09-28 2015-05-26 Singulex, Inc. Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry
CA2709217C (en) * 2007-12-19 2021-01-05 Singulex, Inc. Scanning analyzer for single molecule detection and methods of use

Also Published As

Publication number Publication date
WO2017117113A1 (en) 2017-07-06

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