WO2017108158A1 - Procédé et dispositif de traitement d'un milieu de culture à inoculer dans un milieu support - Google Patents

Procédé et dispositif de traitement d'un milieu de culture à inoculer dans un milieu support Download PDF

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Publication number
WO2017108158A1
WO2017108158A1 PCT/EP2016/002037 EP2016002037W WO2017108158A1 WO 2017108158 A1 WO2017108158 A1 WO 2017108158A1 EP 2016002037 W EP2016002037 W EP 2016002037W WO 2017108158 A1 WO2017108158 A1 WO 2017108158A1
Authority
WO
WIPO (PCT)
Prior art keywords
flow path
carrier medium
culture medium
medium
valve
Prior art date
Application number
PCT/EP2016/002037
Other languages
German (de)
English (en)
Inventor
Joachim LÖW
Original Assignee
Gea Tds Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gea Tds Gmbh filed Critical Gea Tds Gmbh
Publication of WO2017108158A1 publication Critical patent/WO2017108158A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/26—Conditioning fluids entering or exiting the reaction vessel
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C13/00—Cream; Cream preparations; Making thereof
    • A23C13/12—Cream preparations
    • A23C13/16—Cream preparations containing, or treated with, microorganisms, enzymes, or antibiotics; Sour cream
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes

Definitions

  • the invention relates to a method for treating a culture medium to be inoculated into a carrier medium, wherein the carrier medium having a carrier medium temperature is discontinuously seeded with the culture medium having a culture medium temperature at a target site, and a device for Implementation of the method is suitable. Furthermore, an application of the method and a use of the plant are each intended for the production of milk products, in particular of cheese, yogurt, sour cream or other fermented milk products.
  • a liquid or a flowable medium which is enriched with desirable microorganisms and in a main medium
  • the carrier medium either continuously or discontinuously, preferably in a single dose, added or metered or is vaccinated.
  • batches or batches can be successively produced from a product stream which is preferably continuously provided, the aforementioned carrier medium in the form of a total stream of this carrier medium, each batch containing a predetermined amount of culture medium or fermentation culture is added or each batch is inoculated.
  • the addition or inoculation into the respective charge is often not continuous lent over the entire period of generation of the batch, but discontinuous at a certain time in the period of filling the batch, preferably in a single dose.
  • the addition or vaccination can be done either in the batch or in the volume, which is delimited, for example, in a tank or process vessel, directly or it can be in the preferably continuous feed of the carrier medium to the tank or process vessel, wherein the charge or the volume is fed via the inlet.
  • the term “inoculation” also refers to the addition in question and the fermentation culture is sub-summed up under the term culture medium.
  • the amount of culture medium is usually relatively small in relation to the amount of carrier medium that forms the batch or volume; it is up to a few volume or volume percent. While the carrier medium or the total current of the carrier medium prior to the generation of the batch or the volume to a carrier medium temperature, a desired fermentation temperature, depending on the product to be produced, is heated, the culture medium for reasons of quality assurance in the Usually cold, ie at a temperature below the carrier medium temperature, stored.
  • a mixing temperature results that is close to the carrier medium temperature due to the small addition or inoculation amount.
  • the lowering of the mixing temperature due to the addition or inoculation is either compensated for by a sufficient increase in the carrier medium temperature or is often ignored if it is insignificant.
  • WOOO / 72691 A2 in the figure shows the addition of a starter culture 18 to milk medium at a temperature of 40 ° C to 60 ° C in a fermenter 20 for the preparation of a milk product.
  • US 2007/0077332 A1 describes the production of a milk product, wherein the fermentation process according to claim 1 (c) comprises two phases.
  • WO 2008/052629 A1 reproduces the principle of a cascade fermentation with three fermenters in FIG. 4, wherein according to claim 1 c) medium is transferred.
  • the present invention has for its object to provide a method and a system suitable for carrying out the method, which energetically optimize the vaccination process over known solutions, improve the temperature control in the vaccination process and ensure the quality of the culture medium.
  • the method according to the invention is based on a known method for treating a culture medium to be inoculated into a carrier medium, in which the carrier medium, which has a carrier medium temperature, is disintegrated at a destination with the culture medium, which has a culture medium temperature. is continuously inoculated.
  • the culture medium temperature is always colder than the carrier medium temperature.
  • the inventive procedural basic idea when vaccinating or dosing a batch carrier medium with a culture medium is that before and until the initiation of the discontinuous vaccination or dosage of the flow path of the culture medium to the destination, the inoculation or metering, with a partial flow of the carrier medium flows through permanently and so that it is heated to the carrier medium temperature.
  • the culture medium Upon initiation of the vaccination or dosing process, the culture medium substitutes the carrier medium present in this flow path and is thereby heated to the carrier medium temperature by indirect heat exchange.
  • the flow path still acting culture medium is ejected by a partial flow of the carrier medium from this flow path, which then continues the permanent flow of this flow path with the partial flow of the carrier medium until the next vaccination or Dosierzyloses.
  • a vaccination or dosing cycle in the context of the above-outlined inventive method is carried out in the following steps (i) to (vi):
  • a total current carrier medium which is present throughout the vaccination cycle is divided into a first substream carrier medium and into a second substream carrier medium. The division is made consistently and without interruptions.
  • the first substream carrier medium is continued in a main flowpath, the second substream carrier medium is routed via a tributarypath, and both substreams of carrier medium are combined at the destination.
  • the flow through the main and the Mauströmungsweges is continuous and without interruptions.
  • the carrier medium is divided and handled according to steps (i) and (ii), whereby the culture medium still present in the secondary flow path is ejected to the destination with the second partial flow carrier medium.
  • a first embodiment of the method according to the invention provides that the destination at which the vaccination or dosage takes place with the culture medium is a first destination in the form of the total flow carrier medium in the main flow path. This means that the culture medium is inoculated or metered into the flowing carrier medium, which forms the charge of the carrier medium and flows into a space receiving this charge.
  • a second embodiment of the method according to the invention provides that the destination at which the vaccination or dosage takes place with the culture medium is a second destination in the form of the carrier medium located in a vaccine or dosing container. This means that in the course of the batchwise delimitation of the carrier medium, the culture medium is directly inoculated or metered into the forming batch of the carrier medium.
  • a plant of the first type for treating a culture medium to be inoculated into a carrier medium which is suitable for carrying out the method according to the invention, is characterized by the following features:
  • It has a main flow path for the carrier medium, which has a branch flow path branching off at a branch point and a junction point arranged below the branch point.
  • the branch flow path opens.
  • the bypass flow path opens into the main flow path at the junction.
  • a heat exchanger is provided via which a heat exchanger flow path is routed on the primary side and the secondary flow path on the secondary side.
  • a first shut-off valve is disposed, in the bypass flow path, a switching valve is provided upstream of the heat exchanger, into which additionally at least the branch flow path opens, and in the heat carrier flow path, a second shut-off valve is disposed.
  • a control device is provided which is connected to the first signal receiver at least via a first signal line connection and to the second signal receiver via a second signal line connection.
  • the control device is furthermore connected to control line connections at least to the first shut-off valve, the change-over valve, the second shut-off valve and to the bypass pump provided as an alternative to the flow resistance.
  • the flow paths are preferably in the form of pipelines.
  • a plant of the second type for treating a culture medium to be inoculated into a carrier medium which is suitable for carrying out the method according to the invention, is characterized by the following features: It has a main flow path for the carrier medium, which has a branch flow path branching off at a branch point and which opens into a seed container downstream of the branch point.
  • a heat exchanger is provided via which a heat exchanger flow path is routed on the primary side and the secondary flow path on the secondary side.
  • a first shut-off valve is arranged in the branch flow path, in the bypass flow path a change-over valve upstream of the heat exchanger is provided, into which additionally at least the branch flow path opens, and a second shut-off valve is arranged in the heat transfer flow path.
  • Either a flow resistance is provided in the main flow path downstream of the branch point, or a bypass pump is disposed in the bypass flow downstream of the changeover valve, both of which serve to ensure sufficient flow in the bypass flow path parallel to the flow in the main flow path.
  • a control device is provided which is connected to the first signal receiver at least via a first signal line connection and to the second signal receiver via a second signal line connection.
  • the control device is furthermore provided with control line connections at least with the first shut-off valve, the change-over valve, the second shut-off valve and with the alternative to the flow resistance
  • An advantageous embodiment of the plant of the first and second type provides in each case in the secondary flow downstream of the heat exchanger, a control valve. With this control valve, it is possible to ensure a dosage or inoculation of the culture medium adapted to the total flow carrier medium in the main flow path.
  • the method of the present invention can be applied to any liquid or fluid food products prepared and / or treated in processes in which a culture medium is dosed or inoculated. It is particularly suitable for the production of milk products and in particular for the production of cheese, yoghurt, sour cream or other fermented milk products.
  • the first and second type plant according to the invention can be applied to all liquid or flowable food products produced and / or treated in processes in which a culture medium is dosed or inoculated.
  • the plants are particularly suitable for the production of dairy products and in particular for the production of cheese, yogurt, sour cream or other fermented milk products.
  • FIG. 1 shows, in a schematic and essentially reduced representation, a plant according to the invention for treating a culture medium to be inoculated into a carrier medium, in which a secondary flow path at a first destination opens into a main flow path, with all of them for further explanation of the plant and of the invention Method necessary designations;
  • FIG. 3 is a schematic representation of the plant of the first type according to FIG. 1, in which a total flow of carrier medium is in the phase of its division into a first partial flow carrier medium and a second partial flow carrier medium;
  • Figure 4 shows a schematic representation of the plant of the first kind according to Figure 1, wherein the culture medium is in the phase of its warming to carrier medium temperature and the discontinuous inoculation into the total current carrier medium;
  • FIG. 5 is a schematic representation of the installation of the second type according to FIG. 2,
  • a total current carrier medium is in the phase of its division into a first partial flow carrier medium and a second Partström carrier medium
  • FIG. 6 is a schematic representation of the installation of the second type according to FIG. 2,
  • culture medium is in the phase of its warming to carrier medium temperature and the discontinuous inoculation in the total current carrier medium.
  • a plant of the first type 100 (FIG. 1) for treating a culture medium KM to be inoculated into a carrier medium TM which is suitable for carrying out the process according to the invention, has a main flow path 1 to which the carrier medium TM having a carrier medium temperature ⁇ is supplied.
  • the main flow path 1 has a branch flow path 1a branched off at a branch point 6 and a junction point 7 arranged downstream of the branch point 6.
  • the association point 7 acts as the destination Z, in this case as the first destination Z1 in the form of a vaccine or metering point, for the culture medium KM flowing in a secondary flow path 2 in the phase of the vaccination.
  • the plant of the first type 100 has the bypass flow path 2, into which, on the one hand, the branch flow path 1a opens at a switching valve 9 arranged in the secondary flow path 2.
  • the secondary flow path 2 On the other hand, it flows into the main flow path 1 at the junction 7.
  • the upstream of the switching valve 9 located part of the secondary flow path 2 the culture medium KM is supplied with a culture medium temperature ⁇ ( ⁇ ).
  • the main flow path 1 continues, whereby either carrier medium TM with the carrier medium temperature ⁇ or medium TM + KM (mixture of carrier medium TM and culture medium KM) inoculated with culture medium is removed at the carrier medium temperature ⁇ .
  • a heat exchanger 4 is provided, on the primary side a heat carrier flow path 3 and on the secondary side of the secondary flow path 2 is guided.
  • a heat transfer medium WM with a heat transfer medium inlet temperature 01 (WM) is supplied to the heat exchanger 4 via the heat transfer flow path 3, leaving the heat exchanger 4 after heat release via the heat transfer flow path 3 with a heat carrier exit temperature 02 (WM).
  • a first shut-off valve 8 is arranged in the Fineströmungsweg 2 upstream of the heat exchanger 4, the changeover valve 9 is provided, in the additionally at least the Abzweigströmungs- 1a opens, the Mauströmungsweg 2 optionally downstream of the heat exchanger 4, a control valve 11 and in the heat transfer flow path 3, a second shut-off valve 10 is arranged.
  • the switching valve 9 has a further connection, which is opened or closed by means of a third shut-off valve 12.
  • first signal receiver 15 In the main flow path 1 upstream of the branch point 6 there is a first signal receiver 15 and in the bypass flow path 2 upstream of the changeover valve 9 there is a second signal receiver 16 which respectively records properties of the carrier medium TM or culture medium KM located there.
  • a flow resistance 5 or it is in the secondary flow mungsweg 2, downstream of the switching valve 9 and preferably upstream of the optional control valve 11, a bypass pump 5.1 arranged, both measures, flow resistance 5 or bypass pump 5.1, ensuring a sufficient flow in Fineströmungsweg 2, parallel to the flow in the main flow path 1, serve.
  • a control device 14 is provided, which is connected to the first signal receiver 15 at least via a first signal line connection a and to the second signal receiver 16 via a second signal line connection b.
  • the control device 14 is further in the order mentioned via control line terminals c, d, e, f, g, h with the first shut-off valve 8, the switching valve 9, the second shut-off valve 10, the optional control valve 11, the bypass pump 5.1 and the third shut-off valve 12 is connected.
  • the main flow path 1, the branch flow path 1a, the bypass flow path 2 and the heat carrier flow path 3 are preferably each formed as a pipeline.
  • the particular embodiment of the shut-off valves 8, 10, 12, the switching valve 9 and the control valve 11 is not limited to the embodiment shown in Figures 1 to 6. Other embodiments may be chosen, provided that the necessary function deriving from the method according to the invention is ensured.
  • the main flow path 1 Upstream of the branch point 6 and downstream of the junction 7, the main flow path 1 forms a total flow carrier medium Q (TM) and upstream of the changeover valve 9, the bypass flow path 2 forms a stream of culture medium Q (KM).
  • TM total flow carrier medium
  • KM culture medium
  • a system of the second type 101 for treating a culture medium KM to be inoculated into a carrier medium TM, which is suitable for carrying out the method according to the invention, differs from the system of the first type 100 in that the secondary flow path 2 no longer exists at the junction 7 into the main flow path 7, but into a seed or dosing 13.
  • the main flow path 1 also opens separately into the seed or dosing tank 13 a.
  • Both for the main flow path 1 and for the benströmungsweg 2 forms the vaccine or metering 13 and thus there located carrier medium TM a second destination Z2 for the supplied via the main flow path 1 support medium TM and the supplied via the Mauströmungsweg 2 culture medium KM.
  • the further construction of the system of the second type 101 is identical to the construction of the system of the first type (FIG. 1). Reference is made in this regard to the above description of Figure 1.
  • a vaccination or dosing cycle in the context of the inventive method is carried out with a plant of the first type 100 (FIGS. 1, 3 and 4) in the following steps (i) to (vi):
  • the total cycle carrier medium Q (TM) (FIG. 3) with the carrier medium temperature ⁇ throughout the vaccination cycle is divided at branch point 6 into a first partial flow carrier medium Q1 (TM) and into a second partial flow carrier medium Q2 (TM) , The division takes place continuously and without interruptions; both partial streams have the carrier medium temperature ⁇ .
  • the first partial flow carrier medium Q1 (TM) is continued in the main flow path 1 and the second partial flow carrier medium Q2 (TM) with the first shut-off valve 8 opened via the branch flow path 1a branching off at the branch point 6 the Mauströmungsweg 2 via the corresponding switched switching valve fed.
  • the first partial flow carrier medium Q1 (TM) and the second partial flow carrier medium Q2 (TM) are combined at the junction 7, the first target location Z1, and transferred via the continuing main flow path 1 as a total flow carrier medium Q (TM) into a further production plant (not shown).
  • Section for example, a container for the delimitation of a seeded with the culture medium KM batch carrier medium TM removed.
  • the flow through the main and the Volunteerströmungsweges 1, 2 is continuous and without interruptions.
  • a further vaccination cycle with a given, defined amount of culture medium KM is carried out according to steps (i) to (vi) according to FIGS. 3 and 4.
  • a vaccination or Dosierzyklus in the inventive method is carried out with a plant of the second type 101 ( Figures 2, 5 and 6) in the above steps (i) to (vi), wherein now the destination Z for the discontinuous vaccination or dosage of the Carrier medium TM with the culture medium KM is no longer at the junction point 7 according to Figures 1, 3 and 4, the first destination Z1, but a second destination Z2 in the form of in the vaccine or Dosier employer 13 carrier medium TM is.
  • the first destination Z1 has to be exchanged mutatis mutandis for the second destination Z2, in the description of FIG.
  • a planned and sufficient flow through the branch flow path 1a in connection with the bypass flow path 2, parallel to the main flow path 1, as shown in Figures 3 and 5, is ensured by a pressure difference between the branch point 6 and the first or second target location Z1, Z2 ,
  • the flow resistance 5 for example a controllable orifice, arranged with the necessary distribution ratio between the first and the second partial flow carrier medium Q1 (TM), Q2 (TM) is adjustable.
  • the necessary pressure difference can also be generated by the bypass pump 5.1 arranged in the secondary flow path 2.
  • carrier medium TM inoculated with culture medium KM carrier medium TM inoculated with culture medium KM
  • first destination inline vaccination or dosing
  • second destination vaccination or dosing

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  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un procédé et un dispositif de traitement d'un milieu de culture (KM) à inoculer dans un milieu support (TM), le milieu de culture (KM) qui présente une température de milieu de culture (£(KM)) étant inoculé de façon discontinue dans le milieu support (TM) qui présente une température de milieu support (8) au niveau d'un emplacement cible (Z; Z1, Z2). Par rapport aux solutions connues, le procédé et le dispositif destinés à la mise en œuvre dudit procédé doivent améliorer énergétiquement le processus d'inoculation, améliorer la commande de température au cours du processus d'inoculation et garantir la qualité du milieu de culture. A cet effet, le procédé selon l'invention comporte les étapes (i) à (vi) d'un cycle d'inoculation.
PCT/EP2016/002037 2015-12-23 2016-12-02 Procédé et dispositif de traitement d'un milieu de culture à inoculer dans un milieu support WO2017108158A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102015016765.0 2015-12-23
DE102015016765.0A DE102015016765B3 (de) 2015-12-23 2015-12-23 Verfahren und Anlage zur Behandlung eines in ein Trägermedium zu impfenden Kulturmediums

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WO2017108158A1 true WO2017108158A1 (fr) 2017-06-29

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PCT/EP2016/002037 WO2017108158A1 (fr) 2015-12-23 2016-12-02 Procédé et dispositif de traitement d'un milieu de culture à inoculer dans un milieu support

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DE (1) DE102015016765B3 (fr)
WO (1) WO2017108158A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0452240A1 (fr) * 1990-04-13 1991-10-16 Britech S.A. Procédé et dispositif de fermentation semi-continue, notamment pour la fabrication industrielle d'un mélange biologique à base d'acide propionique
WO2000072691A2 (fr) * 1999-05-27 2000-12-07 General Mills, Inc. Produits laitiers et procede de preparation
US20070077332A1 (en) * 2005-09-30 2007-04-05 Kraft Foods Holdings, Inc. Fresh cheese products containing biogenerated flavor components and methods for producing
WO2008052629A1 (fr) * 2006-11-02 2008-05-08 Dsm Ip Assets B.V. Procédé semi-continu de fermentation en cascade pour la production du pantothénate
DE102011016604A1 (de) * 2011-04-09 2012-10-11 Dge Dr.-Ing. Günther Engineering Gmbh Verfahren und Vorrichtung zur Herstellung von Biogas aus Gärsubstrat
EP2578558A1 (fr) * 2011-10-06 2013-04-10 Agroittica Acqua e Sole S.p.A. Procédé pour le recyclage des élements nutritifs pour plants

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0452240A1 (fr) * 1990-04-13 1991-10-16 Britech S.A. Procédé et dispositif de fermentation semi-continue, notamment pour la fabrication industrielle d'un mélange biologique à base d'acide propionique
WO2000072691A2 (fr) * 1999-05-27 2000-12-07 General Mills, Inc. Produits laitiers et procede de preparation
US20070077332A1 (en) * 2005-09-30 2007-04-05 Kraft Foods Holdings, Inc. Fresh cheese products containing biogenerated flavor components and methods for producing
WO2008052629A1 (fr) * 2006-11-02 2008-05-08 Dsm Ip Assets B.V. Procédé semi-continu de fermentation en cascade pour la production du pantothénate
DE102011016604A1 (de) * 2011-04-09 2012-10-11 Dge Dr.-Ing. Günther Engineering Gmbh Verfahren und Vorrichtung zur Herstellung von Biogas aus Gärsubstrat
EP2578558A1 (fr) * 2011-10-06 2013-04-10 Agroittica Acqua e Sole S.p.A. Procédé pour le recyclage des élements nutritifs pour plants

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