WO2017065393A1 - Environmentally friendly alcohol-free water-soluble propolis preparation method using natural honey - Google Patents
Environmentally friendly alcohol-free water-soluble propolis preparation method using natural honey Download PDFInfo
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- WO2017065393A1 WO2017065393A1 PCT/KR2016/007859 KR2016007859W WO2017065393A1 WO 2017065393 A1 WO2017065393 A1 WO 2017065393A1 KR 2016007859 W KR2016007859 W KR 2016007859W WO 2017065393 A1 WO2017065393 A1 WO 2017065393A1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
- A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
- A23L21/25—Honey; Honey substitutes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
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- the present invention relates to a method for producing an environmentally friendly non-alcoholic, water-soluble propolis using natural honey, and is characterized in that it produces a water-soluble propolis using the properties of fructose (Fructose) present in natural honey.
- Natural honey is one of the high sugar concentration foods that contain monosaccharides such as fructose and glucose and is easily absorbed by the body to become an energy source. Natural honey contains about 60 to 70% by weight of invert sugar, such as fructose and glucose, and the higher the content of glucose, the easier the crystal is made. On the contrary, the higher the content of fructose, the more the crystal is inhibited.
- Honey produced in Korea is generally composed of 36 to 43% by weight of fructose, 22 to 30% by weight of glucose and 3 to 4% by weight of disaccharide, sucrose.
- fructose since honey has a higher content of fructose than other foods, most of honey's properties such as sweetness, hygroscopicity, and viscosity are represented by fructose. These fructose properties become stronger at lower temperatures.
- fructose is rapidly soluble in water, and the dissolved aqueous solution does not form a supersaturated solution, and thus does not easily form crystals, thereby increasing the dispersion coefficient and thus may be used to accommodate natural extracts.
- the present invention has developed a technique for receiving propolis using the above properties of honey.
- Propolis also called Russian penicillin or natural penicillin, is a material made by honeybees collecting resin from plants and mixing their secreted saliva with wax. Vegetable resins, waxes, essential oils, pollen, flavonoids and other organic substances And minerals. Bees apply the above propolis to the honeycomb gaps to protect themselves from germs and viruses and to prevent invasion by enemies such as wasps and mice. In addition, it maintains a habitat suitable for spawning and growing young bees and ripening and storing honey. Especially when spawning queen bees, it is also used for disinfection of spawning grounds.
- Propolis has long been used by humans as an anti-pesticide, as evidenced by its use in Egypt in about 3000 BC. Recently, in 1965, French physician Remy Showbang, while studying honey bee-free bacteria, discovered that propolis was a natural antibiotic.
- Propolis contains the most organics and minerals, and contains 104 kinds of ingredients. Among these components, minerals, vitamins, amino acids, fats, organic acids, flavonoids, etc. play an important role in cell metabolism, terpenes, etc. have an anticancer action. In particular, it contains more than 100 kinds of flavonoids, which greatly help the health of the human body.
- the main benefits are anti-inflammatory, antioxidant, and immune boosting.
- the anti-inflammatory effect is due to half the enzymes that make prostagdins that cause inflammation in the body.
- flavonoids the main ingredient, eliminates free radicals, which also have antioxidant effects.
- Quercetin which has anti-cancer effects, also plays a role in blocking cancer cell genes before they are replicated.
- the demand for the propolis is expected to increase due to the availability, and it is difficult to completely remove the wax or resin, and also has problems such as being limited to use for various purposes due to being insoluble in water.
- the propolis ingot was extracted with 95% ethanol, concentrated under reduced pressure, and 5-10 times the weight of the raw material was added, and 10-20% of the total weight of the emulsifier was added to be homogeneous.
- a method of producing a water soluble gel propolis by concentration under reduced pressure after mixing is described.
- Korean Patent Publication No. 2002-76979 propolis ingot was extracted with 95% ethanol, water was added to make a solution of 65-70%, and mixed with sodium citrate to have a pH of 6-7, to remove wax.
- a method for producing a water-soluble propolis with propylene glycol after concentration under reduced pressure is described, and Japanese Laid-Open Patent Publication No. 2001-275587 describes a method for extracting and preparing a propolis stock solution using far infrared rays at low temperature.
- Korean Patent Laid-Open Publication No. 10-1992-0014532 discloses a method for preparing a propolis extract having a high water solubility by adding cyclodextrin to an extract of propolis that is difficult to be water-soluble.
- No. 1 shows a method of preparing a propolis extract using ethanol and using it as a honeycomb fragrance.
- Korean Ginseng Research Institute a method of preparing a propolis extract having a high solubility by adding polysorbate and lecithin (Korean Patent Publication No. 10) -1998-0022075).
- Korean Patent No. 10-486922 a method for producing an alcohol-free water-soluble propolis is mixed with propolis ingot and drinking ethanol in a ratio of 1: 4 by weight and added to the inside of the tank, while stirring with a stirring blade. Extracting and aging for about 7 to 14 days, filtration of the extracted and matured extracts first using a micro filter, and preserving the first filtration extracts in a freezer at minus 20 degrees Celsius for 48 hours. Performing a dewaxing process for separating fat, wax and resin components from the extract, and using a micro filter for the extract to remove the fat, wax and resin components separated through the primary dewaxing process from the extract.
- Korean Patent No. 10-748846 discloses a method for producing a propolis fermented product, which is prepared by adding propolis extract powder to water, inoculating microorganisms, and fermenting the same to obtain a propolis fermented product.
- Korean Patent No. 10-0601395 discloses a method for extracting propolis ingot with alcohol or alcohol
- Korean Patent Laid-Open Publication No. 2001-0017765 discloses a water-soluble pro, using sodium citrate as a PH regulator and propylene glycol as a solvent.
- a method for preparing polis has been disclosed.
- the above-described conventional techniques have a problem in that the manufacturing process is complicated and takes a long time by solubilizing propolis using ethanol or spirits, or accepting it through a fermentation step. .
- the present inventors have developed a method of extracting propolis, from which wax has been removed, with ethanol such that the content of flavonoids is 2% by weight or more, and then adding L-arginine to Korean Patent Registration No. 10-1403511.
- the present invention has been completed by discovering that propolis can be solubilized using fructose of honey as a further improvement of the above-described prior art of the present invention.
- the present invention solves the problem of the process of extracting propolis from the conventional ethanol and removes foreign substances such as foreign substances and waxes contained in the propolis, and then, in the solubilization step, the honey content is obtained through isomerization and alkalizing of honey. It is an object of the present invention to provide a method for producing an environmentally friendly, non-alcoholic water-soluble propolis that has a high solubility in propolis.
- the present invention undergoes the following steps.
- the non-alcoholic water-soluble propolis is composed of manufacturing steps,
- Step 2 Steps to remove wax and resin from propolis
- L-Arginine is added 1.0 to 10% by weight in 2 to 3 times the weight ratio. (In this case, Jungnbi ratio means the result of 1st and 2nd stage)
- the present invention is eco-friendly by using any fructose contained in honey to propolis by using no chemical additives other than natural L-arginine, especially in the manufacturing process is not discharged waste water or pollutants at all,
- the propolis extracted by the method of the present invention does not retain ethanol, so the taste and aroma is soft when ingested, and palatability is improved. Since it is well soluble in water by L-arginine, dilution in water is possible for eating and eating. Bright and transparent, there is no residue left in the manufacturing process has the effect of significantly improving the yield.
- 1 is a culture photograph showing the activity of the control group A and samples B, C, D against Pseudomonas aeruginosa (Pseudomonas aeruginosa).
- Figure 2 is the result of measuring the number of Pseudomonas aeruginosa.
- Figure 3 is a culture photograph showing the activity of Control A and Samples B, C, D in E. coli.
- the present invention is subjected to the following steps as a method for producing an environmentally friendly alcohol-free, water-soluble propolis.
- test solution 0.5 was taken in a test tube, ethanol 1.5, 10% aluminum nitrate solution 0.1, 1 M potassium acetate solution 0.1 and water 2.8 were added and stirred sufficiently. After standing for 40 minutes at room temperature, the absorbance was measured at 415 nm using 10 cells in the liquid layer. As a blank control of the sample, total flavonoids were calculated on the basis of a calibration curve prepared with Quecetin, using 0.1 water instead of aluminum nitrate solution during the above operation as the absorbance difference.
- Antimicrobial activity analysis experiment was conducted by functional food research institute affiliated with Korea Health Functional Food Association, Seongnam-si, Gyeonggi-do.
- Sample B is an ethanol extract propolis extract extracted with 70% ethanol and propolis in a weight ratio of 5: 1, which is the most common method for producing a propolis extract
- sample C is a commercially available British non-vitamin propolis liquid (UK Nature's). Laboratoty Ltd, Lot No.0276)
- Sample D is an environmentally friendly, non-alcoholic water-soluble propolis prepared using natural honey, which is the method of the present invention, and has a total flavonoid content of 15 mg / g.
- Each of these samples was tested for antimicrobial activity against Pseudomonas aeruginosa (Pseudomonas aeruginosa) and Escherichia coli (E. coli).
- Samples B and C of the samples were pretreated with 15 mg / g of flavonoids, the propolis indicator component, in the same manner as Sample D, by diluting the concentrations according to the KFDA. Based on the general antimicrobial test culture method, it was performed in two ways: solid culture method and liquid culture method.
- each of the strain culture solution is coated on the NR agar medium, which is a microbial medium, and the paper disc, which is filled with the test sample, is incubated at 30 ° C for 30 hours, and then there is an inhibition zone around the paper disc. Observation of the size confirmed the antimicrobial activity. All tests were repeated three times.
- 1% (v / v) of the test strain culture solution and 1% (v / v) of the treated sample were simultaneously inoculated into sterilized NR liquid medium, and then incubated for 30 hours while stirring at 180 rpm at 30 ° C. After confirming the bacteria grown with the naked eye, diluted with sterile distilled water at an appropriate magnification, smeared in NR agar medium for viable cell count and incubated for 30 hours at 30 °C and counted the viable counts compared with the control. All experiments were repeated three times.
- the medium used was NR (Nutrient Rich) medium, and the medium composition was beef extract 3.0g, peptone5.0g, Agar15.0g.
- Sample B had a somewhat antimicrobial inhibitory effect, but it was confirmed that the antimicrobial activity is significantly lower than that of Sample D.
- sterile water and sample C had weak growth growth ring formation for both Pseudomonas aeruginosa and Escherichia coli, and as shown in FIGS. 2 and 4, the number of Pseudomonas aeruginosa was significantly increased, indicating that the antibacterial activity was very weak.
- the active ingredient of Sample D according to the present invention was found to have a remarkably excellent inhibitory activity against pathogenic bacteria such as Pseudomonas aeruginosa and E. coli.
- Propolis liquid (tink) dietary supplement is a common intake method of diluting a certain amount of water intake. Therefore, most of domestic and foreign products do not remove resin or wax properly when diluted in water, and because of poor water solubility, the color becomes hazy, and precipitation or resin and wax floats in the upper layer to form a film. And palatability, such as clumping, is greatly reduced.
- composition according to the present invention When the composition according to the present invention is diluted in water, there is no suspension phenomenon and waxes, resins, etc. do not float, thereby showing a clear, bright tan propolis intrinsic color.
- the products (A), (B), (D), and (E), except the composition (C) of the present invention are clouded and suspended.
- the color of the product of the present invention was clear and bright, and the color of the propolis was stabilized to a tan, a unique color of propolis, it was confirmed that the clear and bright.
- propolis which has anti-inflammatory effects such as anti-inflammatory, anti-oxidation, and immunity enhancement, the demand is expected to increase, but it is difficult to completely remove wax or resin and is insoluble in water. Had problems such as.
- the present invention is eco-friendly by using a fructose contained in honey to solubilize propolis, so that other chemical additives are not used in addition to natural L-arginine, and in particular, no waste water or pollutants are discharged during the manufacturing process, Taste and aroma are improved, and palatability is improved, and it is well dissolved in water by L-arginine, so it can be eaten by diluting with water when ingested, and the color is bright and transparent.
- a propolis it can be usefully used as a composition for functional foods and pharmaceuticals.
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Abstract
The present invention relates to a method for preparing environmentally friendly alcohol-free water-soluble propolis comprising: after removing foreign substances and floating materials such as wax contained in proplis, isomerizing and alkalifying honey for water-solubilization of the propolis; stirring, filtering and heating the same; and water-solubilizing the propolis by increasing the fructose content by inserting mineral water and L-arginine.
Description
본 발명은 천연벌꿀을 이용한 친환경 무알콜, 수용성 프로폴리스의 제조방법에 관한 것으로 천연 벌꿀에 존재하는 과당(Fructose)의 특성을 이용하여 수용성의 프로폴리스를 제조하는 점에 특징이 있다. 천연 벌꿀은 과당이나 포도당 등의 단당류를 주성분으로 한 고당농도 식품의 하나이며 체내에서 쉽게 흡수되어 에너지원이 된다. 천연 벌꿀은 과당, 포도당 등 단당류인 전화당이 60~70중량%로 정도 함유되어 있으며, 포도당의 함량이 높을수록 결정이 쉽게 이루어지며 반대로 과당의 함량이 높을수록 결정이 억제된다. 국내에서 생산되는 벌꿀은 일반적으로 과당이 36~43중량%, 포도당이 22~30중량% 정도이고 이당류인 자당은 3~4중량% 정도의 조성 비율로 이루어져 있다. 특히, 벌꿀은 다른 식품에 비하여 과당의 함량이 높기 때문에 감미 및 흡습성 강하고 점성을 갖는 등 벌꿀이 갖는 특성의 대부분 이 과당에 의해 나타난다고 볼 수 있다. 이러한 과당의 특성은 온도가 낮을수록 더 강해진다. 또한 과당은 물에 용해가 빠르며 용해된 수용액은 과포화 용액을 형성하지 않아 쉽게 결정을 형성하지 않음으로써 분산계수를 높여 천연추출물을 수용화하는데 활용할 수 있다. 본 발명은 벌꿀의 상기와 같은 특성을 이용하여 프로폴리스를 수용화하는 기술을 개발하였다.The present invention relates to a method for producing an environmentally friendly non-alcoholic, water-soluble propolis using natural honey, and is characterized in that it produces a water-soluble propolis using the properties of fructose (Fructose) present in natural honey. Natural honey is one of the high sugar concentration foods that contain monosaccharides such as fructose and glucose and is easily absorbed by the body to become an energy source. Natural honey contains about 60 to 70% by weight of invert sugar, such as fructose and glucose, and the higher the content of glucose, the easier the crystal is made. On the contrary, the higher the content of fructose, the more the crystal is inhibited. Honey produced in Korea is generally composed of 36 to 43% by weight of fructose, 22 to 30% by weight of glucose and 3 to 4% by weight of disaccharide, sucrose. In particular, since honey has a higher content of fructose than other foods, most of honey's properties such as sweetness, hygroscopicity, and viscosity are represented by fructose. These fructose properties become stronger at lower temperatures. In addition, fructose is rapidly soluble in water, and the dissolved aqueous solution does not form a supersaturated solution, and thus does not easily form crystals, thereby increasing the dispersion coefficient and thus may be used to accommodate natural extracts. The present invention has developed a technique for receiving propolis using the above properties of honey.
프로폴리스(Propolis)는 러시안페니실린 또는 천연페니실린이라고도 불리는데 꿀벌이 식물로부터 수지(resin)를 수집하여 자신이 분비한 타액과 왁스를 혼합하여 만든 물질로서 식물성 수지, 왁스, 정유, 화분, 플라보노이드 및 기타 유기물과 미네랄 등으로 조성되어 있다. 꿀벌은 상기의 프로폴리스를 벌집의 틈이 난 곳에 발라서 병균이나 바이러스로부터 스스로를 보호하고, 말벌이나 쥐와 같은 적의 침입을 막는다. 또한, 새끼벌의 산란과 성장, 꿀의 숙성과 보관 등에 알맞은 서식처를 유지하도록 하고 있다. 특히 여왕벌의 산란시에는 산란장의 소독용으로도 사용한다.Propolis, also called Russian penicillin or natural penicillin, is a material made by honeybees collecting resin from plants and mixing their secreted saliva with wax. Vegetable resins, waxes, essential oils, pollen, flavonoids and other organic substances And minerals. Bees apply the above propolis to the honeycomb gaps to protect themselves from germs and viruses and to prevent invasion by enemies such as wasps and mice. In addition, it maintains a habitat suitable for spawning and growing young bees and ripening and storing honey. Especially when spawning queen bees, it is also used for disinfection of spawning grounds.
이러한 프로폴리스는 기원전 약 3000년에 이집트에서 사용했다는 기록이 있을 정도로 오래전부터 인류가 화농방지제로서 사용해왔다. 최근에는 1965년 프랑스의 의사 레미 쇼방이 꿀벌의 몸에 박테리아가 없음을 연구하던 중 프로폴리스가 천연항생물질이라는 사실을 밝혀냈다.Propolis has long been used by humans as an anti-pesticide, as evidenced by its use in Egypt in about 3000 BC. Recently, in 1965, French physician Remy Showbang, while studying honey bee-free bacteria, discovered that propolis was a natural antibiotic.
프로폴리스의 성분으로는 유기물과 미네랄이 가장 많으며 이와 함께 104종 정도의 성분이 들어있다. 이들 성분 중에서 미네랄, 비타민, 아미노산, 지방, 유기산, 플라보노이드 등은 세포대사에 중요한 역할을 하며, 테르펜류 등은 항암작용을 한다. 특히 100종류가 넘는 플라보노이드가 들어있어 인체의 건강 증진에 큰 도움을 준다.Propolis contains the most organics and minerals, and contains 104 kinds of ingredients. Among these components, minerals, vitamins, amino acids, fats, organic acids, flavonoids, etc. play an important role in cell metabolism, terpenes, etc. have an anticancer action. In particular, it contains more than 100 kinds of flavonoids, which greatly help the health of the human body.
주요한 효능으로는 항염, 항산화, 면역증강 등이 있다. 항염 효과를 내는 것은 사람의 몸에 염증을 일으키는 프로스타그딘을 만들어내는 효소를 절반까지 줄이기 때문이다. 또 주요 성분인 플라보노이드가 활성산소를 없애기 때문에 항산화 효과도 있다. 항암 효과를 나타내는 케르세틴 등이 있어서 암세포의 유전자가 복제되기 전에 차단하는 역할을 하기도 한다.The main benefits are anti-inflammatory, antioxidant, and immune boosting. The anti-inflammatory effect is due to half the enzymes that make prostagdins that cause inflammation in the body. In addition, flavonoids, the main ingredient, eliminates free radicals, which also have antioxidant effects. Quercetin, which has anti-cancer effects, also plays a role in blocking cancer cell genes before they are replicated.
따라서, 프로폴리스의 유용성으로 인해 그 수요의 증가가 예상되나 및 왁스나 레진을 완벽하게 제거하는데 어려움이 있고 또한 수불용성인 관계로 다양한 용도로 사용하는데 제한을 받는 등의 문제점을 가지고 있었다.Therefore, the demand for the propolis is expected to increase due to the availability, and it is difficult to completely remove the wax or resin, and also has problems such as being limited to use for various purposes due to being insoluble in water.
상기와 같은 문제점을 해결하기 위하여 프로폴리스의 추출방법이나 수용화하는 방법들이 개발되고 있으며, 일례로,In order to solve the above problems, methods of extracting or accepting propolis have been developed. For example,
국내 공개특허공보 제1997-0039866호에는 프로폴리스 원괴를 95% 에탄올로 추출하고 감압 농축한 후 원료 중량의 5~10배의 물을 가하고 전체 중량의 10~20%의 유화제를 첨가하여 균질이 되도록 혼합한 후 감압 농축하여 수용성 겔 상태의 프로폴리스를 제조하는 방법이 기재되어 있다. In Korean Unexamined Patent Publication No. 1997-0039866, the propolis ingot was extracted with 95% ethanol, concentrated under reduced pressure, and 5-10 times the weight of the raw material was added, and 10-20% of the total weight of the emulsifier was added to be homogeneous. A method of producing a water soluble gel propolis by concentration under reduced pressure after mixing is described.
또한, 국내 공개특허공보 제2002-76979호에는 프로폴리스 원괴를 95% 에탄올로 추출하고 65~70%의 용액이 되도록 물을 가한 후 구연산나트륨으로 pH가 6~7이 되도록 혼합하여 왁스를 제거하고 감압농축 후 프로필렌글리콜로 수용성 프로폴리스를 제조하는 방법이 기재되어 있으며, 일본 공개특허공보 제2001-275587호에는 저온에서 원적외선을 이용하여 프로폴리스 원액을 추출 및 제조하는 방법이 기재되어 있다.In addition, in Korean Patent Publication No. 2002-76979, propolis ingot was extracted with 95% ethanol, water was added to make a solution of 65-70%, and mixed with sodium citrate to have a pH of 6-7, to remove wax. A method for producing a water-soluble propolis with propylene glycol after concentration under reduced pressure is described, and Japanese Laid-Open Patent Publication No. 2001-275587 describes a method for extracting and preparing a propolis stock solution using far infrared rays at low temperature.
또한, 국내 공개특허공보 제10-1992-0014532호에 수용성화하기 어려운 프로폴리스의 추출물을 싸이클로덱스트린을 첨가하여 수용성을 높인 프로폴리스 추출물의 제조방법을 개시하고 있으며, 국내 공개특허공보 제90-7005호에 에탄올을 사용하여 프로폴리스 추출물을 제조하고 이를 봉밀향으로 사용하는 방법이 제시되어 있고, 고려인삼연구소에서는 폴리솔베이트, 레시틴을 첨가하여 용해도를 높인 프로폴리스 추출물의 제조방법(국내공개특허 10-1998-0022075호)을 개발한 바 있다.In addition, Korean Patent Laid-Open Publication No. 10-1992-0014532 discloses a method for preparing a propolis extract having a high water solubility by adding cyclodextrin to an extract of propolis that is difficult to be water-soluble. No. 1 shows a method of preparing a propolis extract using ethanol and using it as a honeycomb fragrance.In Korea Ginseng Research Institute, a method of preparing a propolis extract having a high solubility by adding polysorbate and lecithin (Korean Patent Publication No. 10) -1998-0022075).
국내 등록특허 제10-486922호에는 무알콜 수용성 프로폴리스를 제조하는 방법으로 프로폴리스 원괴와 음용이 가능한 에탄올을 중량 기준으로 1:4의 비율로 혼합하여 탱크의 내부에 투입하고, 교반날개로 교반하면서 7 내지 14일정도 추출 및 숙성을 하는 단계와, 추출 및 숙성이 완료된 추출액을 마이크로 필터를 사용하여 1차적으로 여과하는 단계와, 1차 여과가 완료된 추출액을 섭씨 영하 20도의 냉동고에서 48시간 존치시켜서 상기 추출액으로부터 지방, 왁스 및 수지성분을 분리시키는 탈랍공정을수행하는 단계와, 상기 1차 탈랍공정을 통해 분리된 지방, 왁스 및 수지성분을 추출액으로부터 제거하기 위해 상기 추출액에 대하여 마이크로 필터를 사용하여 2차 여과공정을 수행하는 단계 등을 갖는 제조방법이 개시되어 있으나, 여전히 알코올을 이용하여 추출하고 있어 원천적으로 알코올을 완벽히 제거하는 데는 한계가 있을 수밖에 없다. 국내 등록특허 제10-748846호에는 프로폴리스 추출물 분말을 물에 가하고, 미생물을 접종하여 발효시켜 프로폴리스 발효물을 얻는 공정에 의하여 제조되는 것을 특징으로 하는 프로폴리스 발효물의 제조방법이 개시되어 있으며,In Korean Patent No. 10-486922, a method for producing an alcohol-free water-soluble propolis is mixed with propolis ingot and drinking ethanol in a ratio of 1: 4 by weight and added to the inside of the tank, while stirring with a stirring blade. Extracting and aging for about 7 to 14 days, filtration of the extracted and matured extracts first using a micro filter, and preserving the first filtration extracts in a freezer at minus 20 degrees Celsius for 48 hours. Performing a dewaxing process for separating fat, wax and resin components from the extract, and using a micro filter for the extract to remove the fat, wax and resin components separated through the primary dewaxing process from the extract. There is disclosed a manufacturing method having a step of performing a secondary filtration process, but still using alcohol Because it is extracted, there is no limit to the complete removal of alcohol at the source. Korean Patent No. 10-748846 discloses a method for producing a propolis fermented product, which is prepared by adding propolis extract powder to water, inoculating microorganisms, and fermenting the same to obtain a propolis fermented product.
국내 등록특허 제10-0601395호에는 프로폴리스 원괴를 알콜 또는 주정으로 추출하는 방법이 개시되어 있으며, 국내 공개특허공보 제2001-0017765호에는 구연산나트륨을 PH 조절제로, 프로필렌글리콜을 용제로 하여 수용성 프로폴리스의 제조방법이 개시되어 있다, 그러나 상기의 종래 기술들은 에탄올이나 주정들을 사용하여 프로폴리스를 수용화하거나 발효 등의 단계를 거쳐 수용화함으로써 제조공정이 복잡하고 장시간을 소요되는 등의 문제점이 있었다.Korean Patent No. 10-0601395 discloses a method for extracting propolis ingot with alcohol or alcohol, and Korean Patent Laid-Open Publication No. 2001-0017765 discloses a water-soluble pro, using sodium citrate as a PH regulator and propylene glycol as a solvent. A method for preparing polis has been disclosed. However, the above-described conventional techniques have a problem in that the manufacturing process is complicated and takes a long time by solubilizing propolis using ethanol or spirits, or accepting it through a fermentation step. .
이에 본 발명자는 왁스 등이 제거된 프로폴리스를 플라보노이드의 함량이 2중량% 이상이 되도록 에탄올로 추출한 후 L - 아르기닌을 투입하여 수용화하는 방법을 국내 등록특허 제10-1403511호로 개발한 바 있고, 본 발명의 상기의 선행기술을 보다 개량한 것으로 벌꿀의 과당을 이용하여 프로폴리스를 수용화할 수 있다는 사실을 발견하고 본 발명을 완성하게 되었다.Accordingly, the present inventors have developed a method of extracting propolis, from which wax has been removed, with ethanol such that the content of flavonoids is 2% by weight or more, and then adding L-arginine to Korean Patent Registration No. 10-1403511. The present invention has been completed by discovering that propolis can be solubilized using fructose of honey as a further improvement of the above-described prior art of the present invention.
본 발명은 종래의 에탄올로부터 프로폴리스를 추출하는 공정의 문제점을 해결하고 프로폴리스에 함유되어 있는 이물질 및 왁스 등 부유물질들을 제거한 후 수용화 단계에서 벌꿀을 이성질화처리와 알칼리화처리를 통해 과당 함량을 높여 프로폴리스를 수용화하는 친환경 무알코올 수용성 프로폴리스의 제조방법을 제공하는 것을 목적으로 한다.The present invention solves the problem of the process of extracting propolis from the conventional ethanol and removes foreign substances such as foreign substances and waxes contained in the propolis, and then, in the solubilization step, the honey content is obtained through isomerization and alkalizing of honey. It is an object of the present invention to provide a method for producing an environmentally friendly, non-alcoholic water-soluble propolis that has a high solubility in propolis.
상기 목적을 달성하기 위하여 본 발명은 다음과 같은 단계를 거치게 된다.In order to achieve the above object, the present invention undergoes the following steps.
1, 천연 벌꿀의 특성을 이용하기 위한 이성질화처리와 알칼리화처리를 통해 과당 함량을 높이는 단계1, step to increase fructose content through isomerization and alkali treatment to take advantage of the properties of natural honey
2. 프로폴리스에서 왁스와 레진을 완전히 제거하는 단계2. Complete removal of wax and resin from propolis
3. 무알콜 수용성 프로폴리스를 제조 단계로 구성되며, 이를 상세히 기술하면, 3. The non-alcoholic water-soluble propolis is composed of manufacturing steps,
1단계. 천연 벌꿀의 이성질화처리와 알칼리화처리 단계 Stage 1. Isomerization and Alkalization of Natural Honey
1) 식품공전 규격기준에 적합한 천연벌꿀을 선별한다.1) Select natural honey that meets the food code standards.
2) 천연벌꿀과 에탄올을 5~7:5~3 중량비로 교반탱크에 넣는다. 2) Add natural honey and ethanol to the stirring tank at a weight ratio of 5-7: 5-3.
3) 30 ℃이하, 500~1000rpm 조건으로 3~5시간 교반한다. 3) Stir for 3 to 5 hours at 30 ℃ or less and under 500 ~ 1000rpm conditions.
4) 이성질화효소(이온화인산)를 중량비 0.5%~1.0%, L-아르기닌을 중량비 1.0%~5.0%를 투입하고 20~25 ℃에서 500~1000rpm 조건으로 12시간 교반한다.4) Add 0.5% ~ 1.0% of isomerase (ionized phosphoric acid) by weight ratio and 1.0% ~ 5.0% of L-arginine and stir at 500 ~ 1000rpm at 20 ~ 25 ℃ for 12 hours.
5) 과당 함량이 60중량% 이상임을 확인 한 후 100메쉬(Mesh) 이하의 체로 여과를 한다.5) After checking that the fructose content is over 60% by weight, filter it with a sieve of 100 mesh or less.
2단계. 프로폴리스에서 왁스와 레진을 제거하는 단계Step 2. Steps to remove wax and resin from propolis
1) 건강기능식품공전 규격기준에 적합한 프로폴리스를 선별한다.1) Select propolis that meets the standards of health functional food standards.
2) 프로폴리스와 에탄올을 6~8:4~2 중량비로 교반탱크에 투입한다.2) Propolis and ethanol are added to the stirring tank at a weight ratio of 6-8: 4-2.
3) 40~60, 1,000~2000rpm 조건으로 가열하면서 7~8시간 교반한다. 3) Stir for 7-8 hours while heating at 40 ~ 60, 1,000 ~ 2000rpm.
4) 교반 후 급속 냉각하여 12~24시간 정치한 후, 상층부를 걷어낸다.4) After stirring, the mixture was rapidly cooled and left to stand for 12 to 24 hours, after which the upper layer was removed.
5) 다시 40~60℃, 1,000~2000rpm 조건으로 가열하면서 12~24시간 교반한다. 5) Stir for 12 to 24 hours while heating at 40 ~ 60 ℃, 1,000 ~ 2000rpm conditions again.
6) 150 메쉬(Mesh) 이하로 여과를 한다.6) Filtration to 150 mesh or less.
3단계. 무알콜 수용성 프로폴리스 제조 단계Step 3. Alcohol Free Water Soluble Propolis
1) 1단계 결과물 및 2단계 결과물을 5~20 : 95~80 중량비로 교반탱크에 투입한다.1) Input the 1st stage resultant and 2nd stage resultant to the stirring tank at the weight ratio of 5 ~ 20: 95 ~ 80.
2) 생수를 중량비 대비 1:1 비율로 투입한다(여기에서 중랑비는 1단계 및 2단계 결과물을 의미한다)2) Inject bottled water in a ratio of 1: 1 by weight ratio (in this case, the middle rain ratio means the results of the first and second stages)
3) L- 아르기닌을 중량비 대비 1.0~10 중량%를 2~3회에 나누어 투입한다.(여기에서 중랑비는 1단계 및 2단계 결과물을 의미한다)3) L-Arginine is added 1.0 to 10% by weight in 2 to 3 times the weight ratio. (In this case, Jungnbi ratio means the result of 1st and 2nd stage)
4) 40~60 ℃에서 1,000~2000rpm 조건으로 가열하면서 12~24시간 교반한다. 4) Stir for 12 to 24 hours while heating at 40 ~ 60 ℃ under 1,000 ~ 2000rpm condition.
5) 농축기를 사용하여 투입된 에탄올이 잔류하지 않을 때까지 완전히 회수하면서 무알콜 수용성 프로폴리스를 제조한다.5) Prepare an alcohol-free water-soluble propolis while fully recovering the spent ethanol until there is no residual ethanol.
본 발명은 벌꿀에 함유되어 있는 과당을 이용하여 프로폴리스를 수용화함으로써 천연 L-아르기닌 외 어떠한 화학 첨가제도 사용하지 않아 친환경적이며, 특히 제조과정에서 페수나 오염물질들이 전혀 배출되지 않는 장점이 있으며, 또한 본 발명의 방법으로 추출한 프로폴리스에는 에탄올 등이 잔류하지 않아 섭취 시 맛과 향이 부드러워 기호성이 개선되었으며, L - 아르기닌에 의해 물에 잘 용해되므로 섭취 시 물에 희석시켜 취식이 가능하고, 색상이 밝고 투명하며 제조 공정상 잔류물이 남지 않아서 수율이 대폭 개선되는 효과가 있다.The present invention is eco-friendly by using any fructose contained in honey to propolis by using no chemical additives other than natural L-arginine, especially in the manufacturing process is not discharged waste water or pollutants at all, In addition, the propolis extracted by the method of the present invention does not retain ethanol, so the taste and aroma is soft when ingested, and palatability is improved. Since it is well soluble in water by L-arginine, dilution in water is possible for eating and eating. Bright and transparent, there is no residue left in the manufacturing process has the effect of significantly improving the yield.
도1은 녹농균(수도모나스균, Pseudomonas aeruginosa)을 대상으로 대조군A와 시료B,C,D의 활성을 나타낸 배양 사진이다.1 is a culture photograph showing the activity of the control group A and samples B, C, D against Pseudomonas aeruginosa (Pseudomonas aeruginosa).
도2는 녹농균의 수를 측정한 결과이다.Figure 2 is the result of measuring the number of Pseudomonas aeruginosa.
도3은 대장균(E.coli)을 대상으로 대조군A와 시료B,C,D의 활성을 나타낸 배양 사진이며.Figure 3 is a culture photograph showing the activity of Control A and Samples B, C, D in E. coli.
도 4는 대장균의 생균 수 측정 결과이다.4 is a result of measuring the number of live bacteria of E. coli.
본 발명은 친환경 무알콜, 수용성 프로폴리스의 제조방법으로 다음과 같은 단계를 거치게 된다.The present invention is subjected to the following steps as a method for producing an environmentally friendly alcohol-free, water-soluble propolis.
1. 천연 벌꿀의 특성을 이용하기 위한 이성질화 처리와 알칼리화 처리를 통해 벌꿀의 과당 함량을 높이는 단계1.Raising the content of fructose in honey through isomerization and alkalizing to take advantage of the properties of natural honey
2. 프로폴리스에서 왁스와 레진을 완전히 제거하는 단계2. Complete removal of wax and resin from propolis
4. 무알콜 수용성 프로폴리스 제조하는 단계로 구성되며, 이를 보다 상세히 기술하면 4. It comprises a step of preparing an alcohol-free water-soluble propolis, if described in more detail
〈1단계〉. 천연 벌꿀의 이성질화처리와 알칼리화처리 단계 <Stage 1>. Isomerization and Alkalization of Natural Honey
1) 식품공전 규격기준에 적합한 천연벌꿀을 선별한다.1) Select natural honey that meets the food code standards.
2) 천연꿀과 에탄올을 5~7:5~3 중량비로 교반탱크에 넣는다. 2) Add natural honey and ethanol to the stirring tank at a weight ratio of 5-7: 5-3.
3) 30 ℃ 이하에서 500~1000rpm 조건으로 3~5시간 교반한다. 3) Stir for 3 to 5 hours at 500 ~ 1000rpm conditions below 30 ℃.
4) 이성질화효소(이온화인산)를 0.5 ~ 1.0 중량%, L-아르기닌을 1.0 ~ 5.0 중량%를 투입하고 벌꿀의 과당 함량이 60 중량% 이상이 되도록 20~25에서 500~1000rpm 조건으로 12시간 교반한 후 100메쉬(Mesh)의 체로 여과를 한다.4) 0.5 ~ 1.0% by weight of isomerase (ionized phosphoric acid), 1.0 ~ 5.0% by weight of L-arginine and 12 hours at 500 ~ 1000rpm conditions from 20 to 25 to make the fructose content of honey more than 60% by weight After stirring, the mixture is filtered through a 100 mesh sieve.
〈2단계〉 프로폴리스에서 왁스와 레진을 제거하는 단계<Step 2> Removing Wax and Resin from Propolis
1) 건강기능식품공전 규격기준에 적합한 프로폴리스를 선별한다.1) Select propolis that meets the standards of health functional food standards.
2) 프로폴리스와 에탄올을 6~8:4~2 중량비로 교반탱크에 투입한다.2) Propolis and ethanol are added to the stirring tank at a weight ratio of 6-8: 4-2.
3) 40~60℃에서 1,000~2000rpm 조건으로 가열하면서 7~8시간 교반한다. 3) Stir for 7-8 hours while heating at 40 ~ 60 ℃ to 1,000 ~ 2000rpm condition.
4) 교반 후 급속 냉각하여 12~24시간 정치한 후, 상층부를 걷어낸다.4) After stirring, the mixture was rapidly cooled and left to stand for 12 to 24 hours, after which the upper layer was removed.
5) 다시 40~60℃에서 1,000~2000rpm 조건으로 가열하면서 12~24시간 교반한다. 5) Stir for 12 to 24 hours while heating at 40 ~ 60 ℃ to 1,000 ~ 2000rpm conditions.
6) 150 메쉬(Mesh) 이하의 체로 여과를 한다.6) Filter through a sieve of 150 mesh or less.
〈3단계〉 무알콜 수용성 프로폴리스 제조 단계<Step 3> Production step of alcohol-free water-soluble propolis
1) 1단계 결과물과 2단계 결과물을 5~20 : 95~80 중량비로 교반탱크에 투입한다.1) Put the 1st stage result and the 2nd stage result in the stirring tank at a weight ratio of 5 ~ 20: 95 ~ 80.
2) 생수를 중량대비 1:1비율로 투입한다.2) Pour bottled water at a ratio of 1: 1 by weight.
3) L- 아르기닌을 1단계 결과물과 2단계 결과물의 중량 대비 1.0 ~ 10 중량%를 준비한 후 2~3회에 나누어 투입한다.3) Prepare 1.0 ~ 10% by weight of L-arginine with respect to the weights of the 1st and 2nd stage products and divide into 2 ~ 3 times.
4) 40~60℃에서 1,000~2000rpm 조건으로 가열하면서 12~24시간 교반한다. 4) Stir for 12 to 24 hours while heating at 40 ~ 60 ℃ under 1,000 ~ 2000rpm condition.
5) 농축기를 사용하여 투입된 에탄올이 잔류하지 않을 때까지 완전히 회수하면서 무알콜 수용성 프로폴리스를 제조한다.5) Prepare an alcohol-free water-soluble propolis while fully recovering the spent ethanol until there is no residual ethanol.
상기와 같이 제조된 친환경 무알콜, 수용성 프로폴리스의 주요 구성 성분인 페놀성 화합물의 대부분을 차지하는 총 플라보노이드의 함량은 건강기능식품공전에 따라 추출액(프로폴리스 추출물로 약 60 ~ 100에 상당하는 양)을 칭량(Weighing(Capacity), 秤量)하고 90% 에탄올 20를 가해서 용해, 원심분리(3,000rpm, 10min)하였다. 상등액만 취하고 잔류물은 다시 80% 에탄올 8로 3회 세척, 추출하고 전추출액을 합하여 80% 에탄올을 사용하여 전량 50로 해서 시험용액으로 하였다.The total flavonoid content occupying most of the phenolic compounds, which are the main constituents of the eco-friendly alcohol-free and water-soluble propolis, prepared as described above, was determined according to the Health Functional Food Code (propolis extract equivalent to about 60 to 100). Weighing (Capacity) and 90% ethanol 20 were added to dissolve and centrifuged (3,000 rpm, 10 min). Only the supernatant was taken, and the residue was washed three times with 80% ethanol 8, extracted, and the total amount of the total extract was 80% using 80% ethanol to prepare a test solution.
시험 용액 0.5를 시험관에 취하고 에탄올 1.5, 10% 질산알루미늄용액 0.1, 1M 초산칼륨용액 0.1, 물 2.8를 가하여 충분히 교반하였다. 이를 실온에서 40분간 정치한 후, 액층을 10 cell을 사용하여 415nm에서 흡광도를 측정하였다. 시료의 블랭크 대조군으로는 상기 조작중 질산알루미늄용액 대신 물 0.1를 가한 것을 흡광도 차로 이용하여 퀘세친(Quercetin)으로 작성한 검량선에 의거 총플라보노이드를 산출하였다.The test solution 0.5 was taken in a test tube, ethanol 1.5, 10% aluminum nitrate solution 0.1, 1 M potassium acetate solution 0.1 and water 2.8 were added and stirred sufficiently. After standing for 40 minutes at room temperature, the absorbance was measured at 415 nm using 10 cells in the liquid layer. As a blank control of the sample, total flavonoids were calculated on the basis of a calibration curve prepared with Quecetin, using 0.1 water instead of aluminum nitrate solution during the above operation as the absorbance difference.
실험예 1. 항균 활성 실험Experimental Example 1. Antimicrobial Activity
경기도 성남시 소재 (사)한국건강기능식품협회 부설 기능성식품연구원에 의뢰하여 항균 활성 분석 실험을 실시하였다.Antimicrobial activity analysis experiment was conducted by functional food research institute affiliated with Korea Health Functional Food Association, Seongnam-si, Gyeonggi-do.
대조군(A)은 멸균증류수를 이용하였다. 시료B는 프로폴리스추출물을 제조하는 가장 일반적인 방법인 70% 에탄올과 프로폴리스를 중량비 5:1로 하여 추출한 에탄올추출 프로폴리스추출물이며 시료C는 시중에서 구입한 영국산 비바이탈 프로폴리스리퀴드(영국 Nature's Laboratoty Ltd, Lot No.0276)이고 시료D는 본 발명의 방법인 천연 벌꿀을 이용하여 조제된 친환경 무알콜 수용성 프로폴리스로 총 플라보노이드 함량이 15mg/g이다. 이들 각 시료들을 이용하여 주요 병원균인 녹농균(수도모나스균, Pseudomonas aeruginosa), 대장균(E.coli)에 대한 항균 활성 실험을 실시하였다. As a control (A), sterile distilled water was used. Sample B is an ethanol extract propolis extract extracted with 70% ethanol and propolis in a weight ratio of 5: 1, which is the most common method for producing a propolis extract, and sample C is a commercially available British non-vitamin propolis liquid (UK Nature's). Laboratoty Ltd, Lot No.0276), and Sample D is an environmentally friendly, non-alcoholic water-soluble propolis prepared using natural honey, which is the method of the present invention, and has a total flavonoid content of 15 mg / g. Each of these samples was tested for antimicrobial activity against Pseudomonas aeruginosa (Pseudomonas aeruginosa) and Escherichia coli (E. coli).
시료 중 시료B와 시료C는 식약처에서 정한 건강기능식품 공전 시험법에 따라 농도 희석을 통해 프로폴리스 지표성분인 플라보노이드 함량을 시료D와 동일하게 15mg/g으로 전 처리하였고 대조군A는 멸균 증류수를 사용하였으며 일반 항균 시험 배양법에 기초하여 고체배양법과 액체배양법의 2가지 방법으로 실시하였다. Samples B and C of the samples were pretreated with 15 mg / g of flavonoids, the propolis indicator component, in the same manner as Sample D, by diluting the concentrations according to the KFDA. Based on the general antimicrobial test culture method, it was performed in two ways: solid culture method and liquid culture method.
고체배양법은 미생물 배지인 NR agar배지에 균주 배양액을 각각 도말하고 상층부분에 시험 시료를 묻힌 Paper disc를 올려놓고 30℃에서 30시간 배양한 후 paper disc 주위의 생육저지환(inhibition zone) 생성 유무 및 크기를 관찰하여 항균활성을 확인하였다. 모든 시험은 3회 반복하였다.In the solid culture method, each of the strain culture solution is coated on the NR agar medium, which is a microbial medium, and the paper disc, which is filled with the test sample, is incubated at 30 ° C for 30 hours, and then there is an inhibition zone around the paper disc. Observation of the size confirmed the antimicrobial activity. All tests were repeated three times.
액체배양법은 멸균된 NR액체배지에 시험균주 배양액 1%(v/v)와 처리시료1%(v/v)를 동시에 접종 후 30℃에서 180rpm으로 교반하면서 30시간 배양한 후, 탁도를 기준으로 육안으로 자란 균을 확인한 후, 멸균 증류수를 이용하여 적정 배율로 희석한 후, 생균수 측정을 위한 NR agar 배지에 도말하여 30℃에서 30시간 배양 한 후 생균수를 세어서 대조군과 비교하였다. 모든 실험은 3회 반복하였다.In the liquid culture method, 1% (v / v) of the test strain culture solution and 1% (v / v) of the treated sample were simultaneously inoculated into sterilized NR liquid medium, and then incubated for 30 hours while stirring at 180 rpm at 30 ° C. After confirming the bacteria grown with the naked eye, diluted with sterile distilled water at an appropriate magnification, smeared in NR agar medium for viable cell count and incubated for 30 hours at 30 ℃ and counted the viable counts compared with the control. All experiments were repeated three times.
사용배지는 NR(Nutrient Rich)medium이고, 배지 조성은 beef extract 3.0g, peptone5.0g, Agar15.0g이었다.The medium used was NR (Nutrient Rich) medium, and the medium composition was beef extract 3.0g, peptone5.0g, Agar15.0g.
상기 실험 결과는 하기 [표 1]과 같다.The experimental results are shown in the following [Table 1].
시료sample | 녹농균(수도모나스균, Pseudomonas aeruginosa)Pseudomonas aeruginosa (Pseudomonas aeruginosa) | 대장균(E.coli)E. coli |
대조군AControl Group A | -- | -- |
시료 BSample B | ++++ | ++++ |
시료 CSample C | ++ | ++ |
시료 DSample D | ++++++++ | ++++++ |
상기의 실험결과에 의하면 본 발명에 의한 시료 D의 경우 생육저지환 형성이 뚜렷하며, 생육저지환의 크기도 시료 B 및 시료 C에 비해 1.5~2배 이상으로 컸으며, 생균수 측정에 있어서도 유사한 결과를 확인할 수 있었다.According to the above experimental results, in the case of sample D according to the present invention, growth-lowering ring formation was distinct, and the size of growth-lowering ring was 1.5 to 2 times larger than that of sample B and sample C, and similar results were obtained in measuring the number of viable cells. I could confirm it.
시료 B의 경우 다소 항균억제 효과는 있으나, 시료 D에 비하면 항균활성이 현저히 떨어지는 것을 확인할 수 있었다.Sample B had a somewhat antimicrobial inhibitory effect, but it was confirmed that the antimicrobial activity is significantly lower than that of Sample D.
도 1 및 3에서 보는 바와 같이 멸균수와 시료 C는 녹농균 및 대장균 모두에 대해 생육저지환이 약하게 형성되었고, 도 2 및 4에서 보는 바와 같이 녹농균수가 현저히 증가하여 항균 활성이 매우 약함을 확인할 수 있었다.As shown in FIGS. 1 and 3, sterile water and sample C had weak growth growth ring formation for both Pseudomonas aeruginosa and Escherichia coli, and as shown in FIGS. 2 and 4, the number of Pseudomonas aeruginosa was significantly increased, indicating that the antibacterial activity was very weak.
본 발명에 의한 시료 D의 유효성분은 녹농균 및 대장균 등 병원성균에 대하여 현저한 우수한 억제 활성효과가 있는 것으로 확인되었다. The active ingredient of Sample D according to the present invention was found to have a remarkably excellent inhibitory activity against pathogenic bacteria such as Pseudomonas aeruginosa and E. coli.
실험예2 . 본 발명에 의해 제조된 조성물의 색상비교Experimental Example 2. Color Comparison of Compositions Prepared by the Present Invention
프로폴리스 액상형(팅크형) 건강기능식품은 일정량을 물에 희석하여 섭취하는 것이 일반적인 섭취방법이다. 이에 대부분의 국내외 제품들은 물에 희석할 경우 레진이나 왁스가 제대로 제거되지 않았고, 수용화도 제대로 이루어지지 않았기 때문에 색상이 뿌옇게 현탁 현상이 나타나며 침전이 생기거나 레진과 왁스 등이 상층부에 부유하게 되어 막을 형성하거나 덩어리를 이루는 등 기호성을 현저히 떨어뜨린다. Propolis liquid (tink) dietary supplement is a common intake method of diluting a certain amount of water intake. Therefore, most of domestic and foreign products do not remove resin or wax properly when diluted in water, and because of poor water solubility, the color becomes hazy, and precipitation or resin and wax floats in the upper layer to form a film. And palatability, such as clumping, is greatly reduced.
본 발명에 의한 조성물은 물에 희석할 경우 현탁 현상이 없고 왁스, 레진 등이 부유하지 않아 맑고 밝은 황갈색의 프로폴리스 고유 색상을 나타낸다.When the composition according to the present invention is diluted in water, there is no suspension phenomenon and waxes, resins, etc. do not float, thereby showing a clear, bright tan propolis intrinsic color.
이를 비교 실험하고자 시중 백화점에서 판매되고 있는 유명 수입품 4종을 구입하여 비교하였다. To compare this, we bought four famous imported goods that are sold in department stores and compared them.
시료는 다음과 같다. Samples are as follows.
(A) Bee Vital Propolis Liquid(영국, Nature's Laboratory ltd) (A) Bee Vital Propolis Liquid (Nature's Laboratory ltd)
(B) Propolis Liquid(호주, JNS Pharma Pty Ltd)(B) Propolis Liquid (JNS Pharma Pty Ltd, Australia)
(C) 유니크 수용성 프로폴리스(본 발명의 기술로 제조된 제품)(C) Unique water soluble propolis (product made by the technology of the present invention)
(D) Comvita Proppolis Tincture(뉴질랜드, Comvita New Zealand Ltd)(D) Comvita Proppolis Tincture (Comvita New Zealand Ltd)
(E) Tong Life Propolis Liquid(캐나다, Triton Pharmaceutical Ltd(E) Tong Life Propolis Liquid, Triton Pharmaceutical Ltd, Canada
75ml 생수에 각 시료를 1일 섭취량 기준으로 희석하여 관찰 후 확인한 결과 본 발명의 조성물(C)를 제외한 나머지 제품들인 (A), (B),(D),(E)제품들은 뿌옇게 현탁이 되며, 색상이 탁한 반면에 본 발명의 제품은 색상이 맑고 밝으며 프로폴리스 고유의 색상인 황갈색으로 안정되어 맑고 밝아 투명함을 확인할 수 있었다. As a result of diluting each sample in 75ml bottled water based on daily intake, and confirming the results, the products (A), (B), (D), and (E), except the composition (C) of the present invention, are clouded and suspended. On the other hand, the color of the product of the present invention was clear and bright, and the color of the propolis was stabilized to a tan, a unique color of propolis, it was confirmed that the clear and bright.
항염, 항산화, 면역증강 등의 항염 효과를 갖는 프로폴리스의 유용성으로 인해 그 수요의 증가가 예상되나 왁스나 레진을 완벽하게 제거하는데 어려움이 있고 또한 수불용성인 관계로 다양한 용도로 사용하는데 제한을 받는 등의 문제점을 가지고 있었다.Due to the usefulness of propolis, which has anti-inflammatory effects such as anti-inflammatory, anti-oxidation, and immunity enhancement, the demand is expected to increase, but it is difficult to completely remove wax or resin and is insoluble in water. Had problems such as.
본 발명은 벌꿀에 함유되어 있는 과당을 이용하여 프로폴리스를 수용화함으로써 천연 L-아르기닌 외에 타 화학 첨가제도 사용하지 않아 친환경적이며, 특히 제조과정에서 폐수나 오염물질들이 전혀 배출되지 않으며, 또한 섭취 시 맛과 향이 부드러워 기호성이 개선되었으며, L - 아르기닌에 의해 물에 잘 용해되므로 섭취 시 물에 희석시켜 취식이 가능하고, 색상이 밝고 투명하며 제조 공정상 잔류물이 남지 않아서 수율이 대폭 개선된 수용화한 프로폴리스를 제조함으로써 기능성 식품이나 의약의 조성물로 유용하게 사용할 수 있다.The present invention is eco-friendly by using a fructose contained in honey to solubilize propolis, so that other chemical additives are not used in addition to natural L-arginine, and in particular, no waste water or pollutants are discharged during the manufacturing process, Taste and aroma are improved, and palatability is improved, and it is well dissolved in water by L-arginine, so it can be eaten by diluting with water when ingested, and the color is bright and transparent. By producing a propolis, it can be usefully used as a composition for functional foods and pharmaceuticals.
Claims (2)
1단계. 천연 벌꿀의 이성질화처리와 알칼리화처리 단계;Stage 1. Isomerization and alkalizing step of natural honey;
천연꿀과 에탄올을 5~7:5~3 중량비로 교반탱크에 넣고 30℃ 이하에서 500~1000rpm 조건으로 3~5시간 교반하는 제1단계;Putting the natural honey and ethanol in a stirring tank at a weight ratio of 5 to 7: 5 to 3, the first step of stirring for 3 to 5 hours at 500 ~ 1000rpm conditions at 30 ℃ or less;
상기의 1차 교반물에 이성질화효소인 이온화인산 0.5 ~ 1.0 중량%와 L-아르기닌 1.0 ~ 5.0 중량%를 투입하고 벌꿀의 과당 함량이 60 중량% 이상이 되도록 20~25℃에서 500~1000rpm 조건으로 12시간 교반한 후 100메쉬(Mesh)의 체로 여과하는 제2단계;0.5 to 1.0% by weight of isomerization phosphoric acid and 1.0 to 5.0% by weight of L-arginine are added to the first stirring mixture, and 500 ~ 1000rpm condition at 20-25 ° C. to make the fructose content of honey more than 60% by weight. After stirring for 12 hours with a second step of filtering through a 100 mesh (Mesh) sieve;
프로폴리스와 에탄올을 6~8:4~2 중량비로 교반탱크에 투입하여 40~60℃에서 1,000~2000rpm 조건으로 가열하면서 7~8시간 교반한 후 급속 냉각한 후 12 내지 24시간 정치시킨 뒤 상층부를 걷어내고 다시 40~60℃ 1,000~2000rpm 조건으로 가열하면서 12~24시간 교반한 후 150 메쉬(Mesh) 이하의 체로 여과하는 제3단계;Propolis and ethanol were added to a stirring tank at a weight ratio of 6-8: 4-2, stirred for 7-8 hours while heating at 40-60 ° C at 1,000-2000 rpm, and then rapidly cooled, and then left for 12-24 hours. The third step of removing the filtered and stirred for 12 to 24 hours while heating to 40 ~ 60 ℃ 1,000 ~ 2000rpm conditions again and filtered through a sieve of less than 150 mesh (Mesh);
제2단계 결과물과 제3단계 결과물을 5~20 : 95~80 중량비로 교반탱크에 투입하고 다시 생수와 L- 아르기닌를 투입한 후 40~60℃에서 1,000~2000rpm 조건으로 가열하면서 12~24시간 교반하는 제4단계; 이루어지는 것을 특징으로 하는 무알콜 수용성 프로폴리스의 제조방법.The resultant of step 2 and the resultant of step 3 were added to the stirring tank at a weight ratio of 5 to 20: 95 to 80, and bottled water and L-arginine were added again. Fourth step to do; Method for producing an alcohol-free water-soluble propolis, characterized in that made.
제1항에 있어서,The method of claim 1,
제4단계에서 L- 아르기닌의 함량은 제2단계 및 제3단계 결과물의 중량대비 1.0 - 10중량%인 것을 특징으로 하는 무알콜 수용성 프로폴리스의 제조방법.In the fourth step, the content of L-arginine is 1.0 to 10% by weight based on the weight of the results of the second and third steps, the method for producing an alcohol-free water-soluble propolis.
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KR20010104132A (en) * | 2000-05-13 | 2001-11-24 | 박종성 | Pr0cess for the preparation of the drinking products thereof containing of a propolis abstracts with honey |
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