WO2017047583A1

WO2017047583A1 - Live fish waterless transportation method, live fish transportation container, and method for inducing hypopnea state in live fish

Info

Publication number: WO2017047583A1
Application number: PCT/JP2016/076974
Authority: WO
Inventors: 出島袋; 田島　一雄; 道史君塚; 直起道畑
Original assignee: 積水化成品工業株式会社
Priority date: 2015-09-14
Filing date: 2016-09-13
Publication date: 2017-03-23
Also published as: JP6632630B2; JPWO2017047583A1; KR20180043319A; WO2017047107A1; KR102129257B1

Abstract

Provided is a waterless transportation method for fish, whereby operation is simple and live fish in a hypopnea state suffer no shock and have little likelihood of suffering stress. The present invention also provides a live fish transportation container suitable for waterless transportation of live fish. The present invention also provides a method for inducing a hypopnea state in live fish. This live fish waterless transportation method is characterized by including: a husbandry step in which fish F are bred in water at a temperature within a first temperature range R1; an acclimatization step in which the fish F are acclimatized in the live fish transportation container 1 containing water at a temperature within a second temperature range R2 having a maximum temperature R2U that is lower than a minimum temperature R1L for the first temperature range R1; a water-removal step in which water is removed from the live fish transportation container 1; and a waterless transportation step in which the fish F are kept alive in a waterless environment in the live fish transportation container 1 and transported. This live fish transportation container 100 comprises a container main body 10, a lid 50, and a tray 20 and has separated sections 31, 34 formed therein. A bottom plate 23 of the tray 20 comprises a trunk placement section 21 and a tail placement section 22 and has through-holes formed therein 25, 26. Cooling agent housing sections 32, 33 are formed inside the container main body 10. The method for inducing a hypopnea state in live fish includes the husbandry step and the acclimatization step. The husbandry step includes: a husbandry step 1 performed in water at a temperature T_A; and a husbandry step 2 performed in water at a temperature T_B lower than the temperature T_A.

Description

Anhydrous transport method for live fish, container for transporting live fish, and method for inducing hypopnea state in live fish

The present invention relates to a method for transporting live fish without water (anhydrous transport).

The present invention also relates to a container for transporting live fish.
The present invention also relates to a method for inducing hypopnea in live fish.

As a method for transporting live fish, there is conventionally known a method of transporting live fish while swimming using a vehicle equipped with a tank containing water and supplying air into the tank.

Such a method of transporting live fish in water requires transport of water in addition to live fish, and the transport weight is large. For this reason, the amount of live fish that can be carried with respect to the maximum load capacity of the vehicle is small and the transportation efficiency is poor. In particular, if the transportation cost is high and water leaks during transportation, it is not suitable for transportation by aircraft. It was. In addition, live fish may collide with the inner wall of the tank during transportation, and the fish body may be damaged, reducing the commercial value.

In order to solve the above problems, an anhydrous transport method for transporting live fish in the absence of water has been developed. In the case of fishes that are exothermal animals, body temperature decreases similarly when acclimated in low-temperature water, metabolism such as oxygen consumption is suppressed, and the exercise frequency and heart rate of moths are reduced. (Hereinafter sometimes referred to as “hypopnea”). Low-breathing fish can survive for a certain period of time even under anhydrous conditions where it is difficult to exchange oxygen and carbon dioxide by sputum, and can therefore be transported anhydrously while still alive. Therefore, various methods have been proposed in order to induce a hypopnea state in fish.

Non-Patent Document 1 reports conditions for cold anesthesia (corresponding to a hypopnea state) of natural flounder (Paralichthys olivacens). In Non-Patent Document 1, flounder is acclimatized for about 1 to 2 days in seawater at + 10 ° C, which is the water temperature at the time of landing, and then placed in a water tank containing seawater installed in a large refrigerator, and the temperature is controlled. Cold anesthesia was induced by cooling from + 10 ° C to + 1 ° C or + 5 ° C at a rate of -2.0 ° C / hour. The flounder induced in the low-temperature anesthesia state was taken out from the water tank into the air in the large refrigerator, and kept at a temperature condition of + 10 ° C., + 5 ° C. or + 1 ° C. to evaluate the survival rate. As a result, it was confirmed that flounder kept in air at + 5 ° C. survived for a maximum of 27 hours. In order to implement the method disclosed in Non-Patent Document 1, a large refrigerator that can control the temperature at a set cooling rate is required. Moreover, in order to transport the Japanese flounder that is actually in a low-temperature anesthesia state by applying the method described in Non-Patent Document 1, it is necessary to separately prepare a container for anhydrous transportation and transfer the flounder from the water tank to the container.

In Patent Document 1, the temperature of the seawater is kept constant for each temperature of the lowered stage, while maintaining the marine organisms (such as halibut) in the seawater for a predetermined time, and gradually lowering the temperature of the seawater by a predetermined interval. The time for maintaining the temperature at each of the lowered stages and the temperature of the seawater without deviation of the average oxygen consumption consumed by the marine organisms at a predetermined temperature as a starting point. There is disclosed a method for inducing artificial hibernation of marine organisms, including a step of gradually reducing the time for maintaining the temperature of seawater at each stage temperature in accordance with the temperature of each lowered stage. Specifically, in Example 2 of Patent Document 1, halibut is kept in seawater, the temperature of the seawater is maintained at 13 ° C. for 10 minutes, and the water temperature is lowered at 12 ° C. for 20 minutes and at 11 ° C. for 30 minutes. Increased time to maintain. At a water temperature of 6 ° C., it was maintained for 120 minutes, at 5 ° C. for 180 minutes, and at a water temperature of 4 ° C. where the biological rhythm was stopped, it was maintained for 260 minutes in order to minimize stress. After that, the maintenance time was gradually shortened while lowering the temperature of the seawater, the maintenance time was shortened to 180 minutes at 3 ° C, 120 minutes at 2 ° C, 20 minutes at 1 ° C, and finally at -0.2 ° C. Maintained for 15 minutes. In this way, artificial hibernation was induced in the halibut. In order to carry out the method described in Patent Document 1, a special water tank capable of precise temperature control using a computer program is required. In addition, in order to transport marine organisms that have been cooled in the special water tank described in Patent Document 1 and have been put into an artificial hibernation state, it is necessary to separately prepare a container for anhydrous transport and transfer the marine organisms from the water tank to the container.

Patent Document 2 discloses a method for anhydrous transport of fish. Specifically, the method includes a step of slaughtering fish for a first time in an aquarium maintained at a slaughter temperature, and after the first time, the fish is slaughtered. Quenching the fish by moving into a tank maintained at a cold acclimation temperature lower than the temperature, and acclimating the fish for a second time in the tank maintained at the cold acclimation temperature Inducing the fish into a pseudo-hibernation state and further acclimating, and after the second time has passed, the fish is moved to a transport container, and the interior of the transport container has an oxygen concentration of 60 And transporting the fish in the pseudo-hibernating state in an anhydrous environment by transporting the transport container while maintaining the low acclimation temperature and at least the low acclimation temperature. Is the method. The method of Patent Document 2 is not required to use an expensive refrigerated water tank equipped with a temperature control device required in the methods of Non-Patent Document 1 and Patent Document 1, and aims to induce fish into a pseudo-hibernation state at low cost. It has been developed. However, in the method of Patent Document 2, it is necessary to prepare three tanks, that is, a water tank maintained at a resting culture temperature, a water tank maintained at a low temperature acclimation temperature, and a container for transportation. It is necessary to transfer fish manually between them.

JP 2008-188008 A JP 2014-161239 A

Any of the above conventional methods for anhydrous transport of fish had to be transferred to a container for anhydrous transport prepared separately for fish induced in a hypopnea state in an aquarium. For this reason, there existed a subject that work was very complicated. In addition, when transferring from an aquarium to an anhydrous transport container, impacts are applied to live fish in a low-breathing state, so there is a problem that the low-breathing state of live fish is not stably maintained, and there is a problem that stresses live fish there were.

Further, a container suitable for transporting live fish under anhydrous conditions has been demanded.
In addition, there has been a demand for an efficient method for inducing live fish such as flounder into a low respiratory state.

As a means for solving the above problems, the present inventors have found that the following method is effective, and have completed the present invention.

(1) The method for transporting anhydrous live fish according to the present invention comprises:
A livestock raising process for raising fish in water at a temperature within the first temperature range;
An acclimatization step of acclimatizing fish in a live fish transport member containing water at a temperature in a second temperature range where the upper limit temperature is lower than the lower limit temperature of the first temperature range;
A draining step for draining water from the live fish transporting member;
An anhydrous transporting step of transporting fish while allowing them to survive in the live fish transporting member,
The live fish transport member is a live fish transport container or a live fish transport tray.
In the method of the present invention, the acclimatization step for bringing the fish into a low respiratory state is performed in the live fish transport member, and the water is drained and the anhydrous transport step is performed without being transferred between the members. In addition, the workability is high because there is no need for transfer. According to the method of the present invention, since the impact on fish is small, the survival rate after transportation is high.

(2) The method of the present invention preferably includes a cooling step of cooling the fish in water during or after the livestock raising process until the start or midway of the acclimatization process. More preferably, the cooling step stores fish and water in a heat-insulating container, and the cooling step is placed in a refrigerator whose temperature is set lower than the initial temperature of the stored water. Is done.

(3) In the method (2) of the present invention, more preferably, the fish is moved into a live fish transporting member containing water at a temperature T _{1 in} the _first temperature range after or after the breeding process. And the cooling step is a step of cooling the temperature of the water in the live fish transporting member to the temperature T ₂ in the second temperature range after the moving step is completed. More preferably, the member for transporting live fish is a container for transporting live fish, and the container for transporting live fish is a heat-insulating container, and after the cooling step is completed, the container for transporting live fish is A temperature within the second temperature range, for example, a temperature lower than T ₂ or T ₂ (eg, a temperature below T ₂ and from (T ₂ −0.5) ° C. to (T ₂ −40) ° C., Preferably it is less than T ₂ and a temperature from (T ₂ −0.5) ° C. to (T ₂ −25) ° C., more preferably less than T ₂ and (T ₂ −0.5) ° C. to (T _It is carried out by placing in a refrigerator set to a temperature of 2-15) ° C., more preferably (T ₂ -0.5) ° C. to (T ₂ -5) ° C.).

(4) In the method (3) of the present invention, preferably,
The live fish transport member is a live fish transport container,
The moving step is
A step of placing the water temperature T ₁ of in the live fish transport container,
A step of placing fish in a container for transporting live fish and immersing while discharging excess water.

(5) In the method (2) of the present invention, more preferably, after the end of the animal husbandry process, the temperature is lower than the lower limit temperature of the first temperature range and higher than the temperature T _{2 in the second} temperature range. includes a moving step of moving the fish to the live fish transport member in which accommodates the water temperature T _3, the cooling step cools after completion of the transfer step, the temperature of the water during a live fish transfer member into T ₂ step It is. More preferably, the member for transporting live fish is a container for transporting live fish, and the container for transporting live fish is a heat-insulating container, and after the cooling step is completed, the container for transporting live fish is A temperature in the second temperature range where the temperature is lower than the temperature T ₃ , such as a temperature lower than T ₂ or T ₂ (eg, less than T ₂ and (T ₂ −0.5) ° C. to (T ₂ −40) ) Temperature up to 0 ° C., preferably below T ₂ and (T ₂ −0.5) ° C. to (T ₂ −25) ° C., more preferably below T ₂ and (T ₂ −0. 5) It is carried out by placing in a refrigerator set at a temperature of from 0 ° C. to (T ₂ −15) ° C., more preferably from (T ₂ −0.5) ° C. to (T ₂ −5) ° C.).

(6) The method of (5) of the present invention is preferably
The live fish transport member is a live fish transport container,
The moving step is
A step of placing the water temperature T ₃ in the live fish transport container,
Placing fish in a container for transporting live fish and immersing while discharging excess water;
including.

(7) In the method according to any one of (2) to (6) of the present invention, preferably, the cooling step is performed during the period from the middle or the end of the animal breeding step to the beginning or the middle of the acclimatization step. This is a step of cooling in water by changing the water temperature at a rate of −0.1 to −10 ° C./hour.

(8) In the method according to any one of (2) to (7) of the present invention, preferably, the cooling step is performed during the period from the middle or the end of the animal breeding step to the beginning or the middle of the acclimatization step. This is a step of cooling in water so that the absolute value of the change rate of the water temperature continuously decreases with time.

(9) In any one of the methods (1) to (8) of the present invention, preferably, the upper limit temperature and the lower limit temperature of the first temperature range are in the range of 8 to 30 ° C.

(10) In the method according to any one of (1) to (9) of the present invention, preferably, the livestock raising step raises fish in water at a temperature equal to or lower than a threshold temperature P within the first temperature range. Including
The threshold temperature P is a temperature within a range of 8 ° C. or more and 15 ° C. or less.

(11) The method of (10) of the present invention, preferably, the farming process, a farming step 1 of farmed fish in water of the first temperature region of the temperature T _A, after the farmed step 1, the and a farming step 2 of farmed fish in water at low temperature T _B than the temperature T _a of the first temperature range.

(12) In the method of any one of (1) to (11) of the present invention, preferably, the upper limit temperature and the lower limit temperature of the second temperature range are in the range of −2 to + 7 ° C.

(13) In the method according to any one of (1) to (12) of the present invention, preferably, the first temperature range and / or the second temperature range is set according to a temperature of water at the time of catch of fish. And a water temperature setting step for setting.

(14) In the method according to any one of (1) to (13) of the present invention, preferably, the live fish transporting member is a live fish transporting container, and the live fish transporting container is a heat-insulating plastics material. It is.

(15) In the method of (14) of the present invention, preferably, the plastics material having heat insulation is foamed plastics.

(16) In the method of any one of (1) to (15) of the present invention, preferably, the live fish transporting member is a live fish transporting container, and the anhydrous transporting step keeps the inside of the live fish transporting container cold. It is performed by cooling with an agent.

(17) In any one of the methods (1) to (16) of the present invention, preferably, the anhydrous transport step is performed with an oxygen concentration in the live fish transporting member being 35% or more.

(18) In the method according to any one of (1) to (17) of the present invention, preferably, the fish is a fish belonging to the flounder family.

(19) A container for transporting live fish according to the present invention for containing live fish in an anhydrous manner,
A container body having a bottom wall and a side wall erected from the periphery of the bottom wall; a lid for closing the opening of the container body; a tray having a bottom plate provided in the container body and on which live fish is placed; With
A spacing portion is formed between the tray and the side wall of the container body,
The bottom plate of the tray has a trunk placement portion and a tail placement portion,
A through hole is formed in the bottom plate of the tray,
The container body is characterized in that a cryogen storage portion is formed in the vicinity of the tail placement portion of the tray.
By using the container for transporting live fish of the present invention, the survival rate of live fish in anhydrous transport can be increased.

(20) In the live fish transport container according to (19) of the present invention, preferably, a spacing portion is further formed between the tray and the bottom wall of the container body.

(21) In the live fish transport container according to (19) or (20) of the present invention, preferably, the bottom plate of the tray has a flat plate-like trunk mounting portion having a rectangular shape in plan view, and a short side of the trunk mounting portion. A tail mounting portion extending from one and having a width smaller than that of the body mounting portion, and the through hole is formed in the body mounting portion.

(22) In the live fish transport container according to any one of (19) to (21) of the present invention, preferably, the tray has a side plate erected from a peripheral edge of a bottom plate of the tray, and the side plate Is low.

(23) In the live fish transport container according to any one of (19) to (22) of the present invention, preferably, the bottom plate of the tray is opposite to the tail mounting portion from the center of the trunk mounting portion. A dent is formed near the direction.

(24) In the live fish transporting container according to any one of (19) to (23) of the present invention, preferably, a plurality of the trays are provided, and the trays are stacked with the tail placement portion directed in the same direction. It has been.

(25) In the live fish transport container according to any one of (19) to (24) of the present invention, preferably, a plurality of the trays are provided, and the trays are arranged with the tail placement portions facing in the same direction. It has been.

(26) In the live fish transport container according to any one of (19) to (25) of the present invention, preferably, the container main body includes a portion where a through hole can be formed or an openable / closable drain port.
(27) The method of inducing a hypopnea state in live fish according to the present invention is as follows:
A livestock raising process for raising fish in water at a temperature within the first temperature range;
Acclimating fish in water at a temperature in a second temperature range where the upper limit temperature is lower than the lower limit temperature of the first temperature range,
The farming process, the a farming step 1 of farmed fish in water of the first temperature region of the temperature T _A, after the farmed step 1, the first temperature range, the temperature T is lower than the _A temperature T _B It includes at least a livestock raising process 2 for raising fish in the water.
In this aspect of the present invention, a farming step 1 of farmed fish in water at a temperature T _A as farmed step performed prior to the habituation process, farming step of farmed fish in water at low temperature T _B than the temperature T _A 2 is able to appropriately induce a resting state in which physiological metabolic functions and spontaneous movements are suppressed and vitality is reduced in fish, so that the fish may be stressed in the subsequent acclimation process. It is possible to induce a hypopnea state while reducing. As a result, the stress received by live fish can be reduced, and the possibility that the quality of live fish will be impaired can be reduced.
(28) The method of (27) of the present invention, preferably, the temperature at which the temperature T _A exceeds a threshold temperature P, the temperature T _B is less temperature threshold temperature P, the threshold temperature P is 8 ° C. over 15 It is a temperature within a range of ℃ or less.
(29) In the method of (27) or (28) of the present invention, preferably, the fish is a fish belonging to the family Flounder.
(30) In any of the methods of the present invention (27) to (29), preferably, after the completion of farmed step 1, fish, and starts the farmed step 2 is moved in the water temperature T _B.
(31) In any one of the methods (27) to (30) of the present invention, preferably, the acclimation step is performed while supplying air or oxygen into water.
(32) In the method according to any one of (27) to (31) of the present invention, preferably, the temperature of the fish is continuously changed in water during or after the livestock raising process until the start or middle of the acclimatization process. And a cooling step of cooling while reducing the temperature.
(33) In the method of any one of (27) to (32) of the present invention, preferably, the upper limit temperature and the lower limit temperature of the first temperature range are in the range of 8 to 30 ° C.
(34) In any one of the methods (27) to (33) of the present invention, preferably, the upper limit temperature and the lower limit temperature of the second temperature range are in the range of −2 to + 7 ° C.
(35) In the method according to any one of (27) to (34) of the present invention, preferably, the first temperature range and / or the second temperature range is set according to a temperature of water at the time of catch of fish. And a water temperature setting step for setting.

This specification includes the disclosure of Japanese Patent Application No. 2015-181238 and International Application No. PCT / JP2016 / 050948, which are the basis of the priority of the present application.

In the anhydrous transport method for fish according to the present invention, there is no impact on low-breathing fish, and there is no need to transfer, so workability is high. According to the method of the present invention, since the impact on fish is small, the survival rate of the fish after transportation is high.

The container for transporting live fish of the present invention is suitable for anhydrous transport of live fish.
According to the method for inducing a hypopnea state in live fish according to the present invention, the hypopnea state can be induced while reducing the possibility of stressing the fish, and the possibility that the quality of the fish meat of the live fish is impaired is reduced. be able to.

FIG. 1 shows a container body 10 included in a container 1 for transporting live fish. FIG. 2 shows a tray 20 for placing live fish. FIG. 3 shows a package 100 in which four trays 20 on which live fish F are placed are placed in a live fish transport container 1. FIG. 4 shows the measurement results of the temperature of seawater around fish in the cooling process and the acclimation process in Example 1 and Comparative Example 1. FIG. 5 shows the results of a preliminary test for confirming the relationship between the number of flounder salmon movements and the seawater temperature. In the figure, “May” indicates a measured value of Japanese flounder caught in May, and “Sep” indicates a measured value of Japanese flounder caught in September. The horizontal axis indicates seawater temperature (° C). The vertical axis shows the number of wrinkle exercises per minute. FIG. 6 shows a diagram of the water temperature change in a preferred embodiment of the method of the present invention. FIG. 7 shows a diagram of the water temperature change in another preferred embodiment of the method of the present invention. FIG. 8 shows a diagram of the water temperature change in another preferred embodiment of the method of the present invention. FIG. 9 shows a diagram of water temperature change in another preferred embodiment of the method of the present invention. The photograph of FIG. 10 shows the half of the flounder after the live fish transportation according to Example 2 on the left, and the half of the flounder after the live fish transportation according to Example 3 on the right. The photograph of FIG. 11 shows the half of a flounder after transporting live fish according to Example 4 on the left, and the half of the flounder after transport of live fish according to Comparative Example 2 on the right. FIG. 12 is a perspective view of an embodiment of the live fish transport container 1 containing four trays 20 shown in FIGS. 1 to 3, with the live fish F and the buffer sheet S removed from the perspective view shown in FIG. It is. FIG. 13 is a cross section taken along line XX of FIG. FIG. 14 shows a tray 60 for placing live fish. FIG. 15 is a schematic diagram schematically illustrating a state in which live fish are stored in a live fish transport tray and a livestock raising step, a cooling step, and / or a habituation step are performed.

<Fish>
In the present invention, the fish to be transported by live fish is not limited to a specific species and may be a saltwater fish or a freshwater fish. Examples of the saltwater fish include fish belonging to the flounder family, fish belonging to the flounder family, fish belonging to the Thai family, fish belonging to the grouper, fish belonging to the pufferfish family, and the like. Examples of the fish belonging to the flounder family include flounder, damselfly, scallop, garlic, megalei and the like, and flounder (Paralichthys olivacens) is particularly preferable. Examples of the fish belonging to the flounder family include flounder, black flounder, black flounder, halibut, holly flounder, pine flounder, and the like. Examples of fish belonging to the Thai family include red sea bream and black sea bream. Examples of the fish belonging to the grouper include Yaito Grouper, Que, Ara and the like. Examples of fish belonging to the pufferfish family include trough pufferfish and mahugu. Examples of freshwater fish include carp, crucian carp, eel, loach and catfish.

In the present invention, “water” means water in which the target fish can live. When the target fish is saltwater fish, it indicates seawater, and when the target fish is freshwater fish, freshwater is used. Point to.

<Live fish transportation parts>
The member for transporting live fish used in the method of the present invention is a member capable of accommodating live fish and water and configured to be able to drain and to perform a acclimation process, a draining process, and an anhydrous transport process. If there is no particular limitation. The live fish transport member includes a live fish transport container that can hold live fish and water alone, and a live fish transport tray on which live fish can be placed.

In the present invention, the live fish transport tray may be at least a tray on which live fish can be placed, and need not have a structure capable of holding water alone. When the live fish transport tray is used, the livestock feeding process, the cooling process, or the acclimatization process of the present invention is performed in a state where the live fish transport tray on which live fish is placed is immersed in water contained in another container (for example, an aquarium). be able to. Specifically, as shown in FIG. 15, in this specification, a state in which a live fish transport tray 20 on which live fish F is placed is immersed in water W accommodated in another container 100 is used for transporting live fish. It is said that the water W and the live fish F are “contained” in the tray 20, and the water W around the live fish F in the live fish transport tray 20 in this state is referred to as “water in the live fish transport tray” or “for live fish transport”. It is called “water in the member”. The live fish transport tray is not limited to a shape (dish shape) including a bottom plate and a side plate erected on the periphery of the bottom plate, as in the later-described live fish transport tray 20, and does not have a side plate but only from the bottom plate. The shape of the board which becomes may be sufficient, and the shape of a sheet | seat like the buffer sheet S mentioned later may be sufficient. When the live fish is placed on the live fish transport tray, a sheet such as a buffer sheet may be interposed between the live fish transport tray and the live fish.

In the present invention, a live fish transport container which is an example of a live fish transport member has a structure capable of holding live fish and water alone. For example, a container in which a container body having an opening in which a housing space capable of housing live fish and water is formed and a lid body that can close the opening of the container body is useful as a container for transporting live fish. . In a preferred embodiment of the container, the container main body includes a portion (for example, a thin portion 11 described later) capable of forming a through-hole or an openable / closable drain port, and the through-hole is formed or the drain port is opened at the site. Thus, the storage space of the container communicates with the outside of the container, and the water in the storage space can be discharged.

A preferred embodiment of a container for transporting live fish will be described with reference to FIGS.

As shown in FIG. 3, the live fish transport container 1 includes a container body 10 and a lid 50.

The container body 10 is a bottomed container having a substantially rectangular bottom wall 12 and a side wall 13 erected on the periphery of the bottom wall 12 as shown in FIG. At the upper end of the side wall 13 is formed an upper end ridge 16 that goes around the opening of the container body 10.

A plurality of side wall ridges 14 extending in the height direction are formed on the inner surface of the side wall 13 of the container body 10. The side wall ridges 14 are spaced apart from each other in a direction substantially perpendicular to the extending direction.

A plurality of spacers 15 are provided on the surface of the bottom wall 12 of the container body 10 facing the container. The spacer 15 is a ridge that protrudes from the surface of the bottom wall 12 and extends from an arbitrary side wall 13 toward the side wall 13 opposed to the side wall 13.

The material of the container body 10 is preferably a heat insulating material in terms of heat retention and temperature control in the container. As the heat insulating material, a heat-insulating plastic material (resin material) is preferable, and a foamed plastic material is more preferable. Examples of the foamed plastic material include polystyrene resin foam, polyethylene resin foam, polypropylene resin foam, polyurethane resin foam, and polyester resin foam. The range of the expansion ratio of the foamed plastic material is preferably 10 to 100 times (the density is 0.01 g / cm ³ to 0.1 g / cm ³ ).

The density and the expansion factor are calculated as follows.
Density (g / cm ³ ) = mass of foamed plastic material sample (g) / apparent volume of foamed plastic material sample (cm ³ )
Foaming multiple (times) = 1 / density (g / cm ³ )

In the container body 10, a thin wall portion 11 having a wall surface thinner than other portions is formed near the portion of the side wall 13 that intersects the bottom wall 12. Since the wall portion of the thin wall portion 11 is thinner than other portions of the side wall 13, the thin wall portion 11 can be easily cut out from the outside of the container body 10 using a cutter or the like. Part or all of the cut-out thin portion 11 serves as a through hole that connects the inside and the outside of the housing space (the space surrounded by the bottom wall 12 and the side wall 13) of the container body 10. The water stored in the storage space of the container body 10 until the acclimatization process is completed can be discharged outside the container by cutting out part or all of the thin portion 11 of the container body 10 to form a through hole. It is.

The lid 50 has a shape capable of closing the opening of the container body 10, and preferably has a quadrangular shape in plan view as illustrated. By closing the opening of the container body 10 with the lid 50, the flow of gas and liquid inside and outside the accommodation space through the opening of the container body 10 can be substantially prevented. Further, in the cooling process and the anhydrous transport process, the container body 10 can be covered with the lid 50 to maintain the live fish transport container 1 in a dark state, and the stress applied to the live fish can be reduced.

A lid through hole 51 is formed in the vicinity of an arbitrary corner of the lid 50, and a lid through hole 52 is also formed in the vicinity of the corner on the diagonal side of the corner.

Although not shown in FIG. 3, a concave groove that fits with the upper ridge 16 of the container body 10 is formed on the periphery of the surface of the lid 50 that faces the opening of the container body 10 when the lid 50 is mounted on the container body 10. Is formed. Specifically, as shown in FIG. 13, the upper edge ridge 16 of the container body 10 and the peripheral edge of the surface 50 a that faces the opening of the container body 10 when the cover body 50 is attached to the container body 10. A recessed groove 53 to be fitted is formed.

As the material of the lid 50, the same material as that of the container body 10 can be used.

FIG. 2 shows a tray 20 on which live fish individuals are placed in the live fish transport container 1.

The tray 20 has a bottom plate 23 and side plates 24 erected from the periphery of the bottom plate 23.

The bottom plate 23 is a flat plate-like body placing portion 21 having a rectangular shape in plan view, and a tail placing portion extending from one of the short sides of the body placing portion 21 and having a smaller width than the body placing portion 21. 22. Bottom plate corner through-holes 25 are formed in the vicinity of the four corners of the trunk mounting portion 21, and a bottom plate central through-hole 26 is formed in the vicinity of the center.

As shown in FIG. 2,

notches

24 a ₁ and 24 a ₂ toward the bottom plate 23 are formed on the side plates 24 on both sides in the width direction of the body placing portion 21. That is, the side plates 24 on both sides in the width direction of the body placing portion 21 are partially lowered. Similarly, notches 24b ₁ and 24b ₂ toward the bottom plate 23 are formed in the side plates 24 on both sides in the width direction of the tail placement portion 22, respectively. That is, the side plates 24 on both sides in the width direction of the tail placement portion 22 are partially lowered.

Further, as illustrated in FIG. 2, a recess 28 is formed in the bottom plate 23 from the center of the body mounting portion 21 toward the opposite direction to the tail mounting portion 22. The recessed portion 28 has a substantially rectangular shape in plan view having the same longitudinal direction as that of the body placing portion 21, a stepped portion 27 that is recessed with respect to the periphery of the recessed portion 28, and a tail placing portion that is more than the stepped portion 27. It consists of a bottom plate central through hole 26 formed closer to 22.

The size and shape of the tray 20 can be appropriately set according to the size and shape of the live fish placed on the tray 20. Moreover, although the illustrated tray 20 has a shape in which one live fish is placed, it can be appropriately set to a shape in which two or more live fish can be placed.

FIG. 14 shows a tray 60 on which two live fish are placed in the live fish transport container 1.

The tray 60 has a bottom plate 63 and a side plate 64 erected from the periphery of the bottom plate 63.

The bottom plate 63 is extended along the shape of the live fish from two of the plate-like body placing part 61 in which two horizontally long octagons are connected in plan view and the short side of the body placing part 61. Two tail mounting parts 62 having a smaller width than the body mounting part 61 are provided. Two bottom plate central through-holes 66 are formed in the vicinity of the center of the trunk mounting portion 61.

As shown in FIG. 14, the side plates 64 erected from the peripheral edge of Dono portion 61, _64a 1 notch towards the bottom plate _{_{_{63, 64a 2, 64a 3,}}} 64a 4, 64b 1, 64b 2, 64b 3 , 64c ₁ , 64c ₂ and 64c ₃ are respectively formed. That is, the side plate 64 erected from the peripheral edge of the body placing portion 61 is partially lowered. Similarly, notches 64d ₁ , 64d ₂ , 64d ₃ , and 64d ₄ directed to the bottom plate 63 are formed on the side plate 64 erected from the peripheral edge of the tail placement portion 62, respectively. That is, the side plate 64 of the tail placement portion 62 is partially lowered.

Further, as shown in FIG. 14, the bottom plate 63 is formed with two recessed portions 68 from the center of the body mounting portion 61 toward the opposite direction to the tail mounting portion 62. The recess 68 has a substantially rectangular shape in plan view, a stepped portion 67 that is recessed with respect to the periphery of the recess 68, and a bottom plate center through-hole 66 that is formed closer to the tail placement portion 62 than the stepped portion 67. Consists of.

Further, as shown in FIG. 14, of the periphery of the trunk mounting portion 61, the notches 64 a ₁ and 64 a ₂ , the notches 64 a ₃ and 64 a ₄ , the notches 64 b ₁ and 64 c ₁ , and the notches between 64b ₃ and 64c _3, no side plate 64 is formed. Also, of the peripheral edge of the ax part 62, between the notches 64d ₁ and 64d _2, between the notches 64d ₃ and 64d _4, no side plate 64 is formed.

Although not shown in FIG. 14, one live fish has live fish heads on the sides of the notches 64a ₁ and 64a ₂ and live fish tails on the sides of the notches 64d ₁ and 64d ₂ , The head of the live fish is placed on the side of the notches 64a ₃ and 64a ₄ , and the tail of the live fish is placed on the sides of the notches 64d ₃ and 64d ₄ .

The material of the

trays

20 and 60 may be the same as or different from the material of the container body 10 or the lid 50. Examples of the material different from the material of the container body 10 or the lid 50 include non-foaming resin materials (polystyrene resin, polyethylene resin, polypropylene resin, and the like).

As shown in FIG. 3, a packaging body 100 in which the live fish F is accommodated in the live fish transport container 1 is configured by closing the opening of the container main body 10 that accommodates four trays 20 on which the live fish F is placed, with a lid 50. Is done.

Each of the four trays 20 on which the live fish F are placed is stacked in two stages with the tail placement portion 22 facing the same direction, and is arranged in two rows in the width direction of the tray 20.

Between the tray 20 and the side wall 13 of the container body 10, a side wall separation portion 31 is formed by interposing the side wall ridges 14, and a flow path through which liquid and gas can pass is secured. Although not shown in FIG. 3, similarly, a spacer 15 is interposed between the tray 20 and the bottom wall 12 of the container body 10 to form a bottom wall separation portion, and liquid and gas can pass therethrough. A flow path is secured. Specifically, as shown in FIG. 13, a bottom wall separation portion 34 is formed between the tray 20 and the bottom wall 12 of the container body 10 by the spacer 15 interposed. The means for forming the bottom wall separation portion is not limited to the spacer 15, and for example, a protrusion or a protrusion can be provided on the bottom surface of the tray 20.

The container main body 10 has a space surrounded by the bottom wall 12 and the side wall 13 and the four trays 20 arranged in two rows and two rows (that is, the tail placement portion 22 of the adjacent trays 20 arranged in two rows. A space between the two is formed, and this space serves as the first cold-reserving agent accommodating portion 32.

Further, the container body 10 has a space surrounded by the bottom wall 12 and the side wall 13 and the two trays 20 arranged in two stages (that is, sandwiching the first cryogen storage part 32 and the tail placement part 22). Two spaces in the vertical position) are formed, and this space is used as the second cold storage agent accommodating portion 33.

Further, a gap 35 is formed between the trays 20 stacked in two stages by the

notches

24a ₁ , 24a ₂ , 24b ₁ , 24b ₂ in the side plates 36 on both sides in the width direction of the tray 20, and a flow path is secured. Yes.

When placing the live fish F on the tray 20, it is preferable to place a buffer sheet S on the bottom plate 23 and place the live fish F on the buffer sheet S as shown in FIG.

The buffer sheet S has a shape that matches the trunk placement portion 21 and the tail placement portion 22, and covers the entire surface of the bottom plate 23. By passing the buffer sheet S, while the live fish F is accommodated in the live fish transport container 1, the addition of the own weight applied to the live fish F is further reduced, and the survival rate in the anhydrous transport is further increased. In addition, since the impact applied to the live fish F is eased, the live fish F is hardly damaged.

The thickness of the buffer sheet S is preferably 2 to 20 mm.

The material of the buffer sheet S includes, for example, a resin foam sheet. Examples of the resin foam sheet include a polyurethane resin foam sheet, a polystyrene resin foam sheet, a polyethylene resin foam sheet, and a polypropylene resin foam sheet.

Although not shown, the method of the present invention may be carried out by directly storing the live fish F in the storage space of the container body 10 of the live fish transport container 1 without using the tray 20 and the buffer sheet S.

In the live fish transport container 1 of the present embodiment, a side wall separation portion 31 is formed between the tray 20 and the side wall 14, and the bottom plate corner penetrates the bottom plate 23 of the tray 20 (near the four corners of the trunk mounting portion 21). A hole 25 is formed. Thereby, the cold air or the gas filled with the cold-retaining agent can easily flow through the entire live fish transport container 1. Therefore, the temperature distribution and gas concentration distribution in the live fish transport container 1 become uniform.

In addition, in the vicinity of the tail placement portion 22 of the tray 20, cold

insulation storage portions

32 and 33 are formed. Thereby, the live fish F is gradually cooled from the tail side. For this reason, live fish are less likely to be cooled excessively on the head side where the internal organs (heart, etc.) are located.

Therefore, according to the live fish transport container 1 of the present embodiment, the survival rate of the live fish in anhydrous transport can be further increased.

Further, in the draining step in the method for transporting anhydrous live fish according to the present invention, when the water is discharged from the live fish transport container 1, the side wall separating portion 31 and the bottom plate corner through hole 25 serve as a water flow path, so that water is discharged. Is easy.

In the live fish transport container 1 of the present embodiment, a bottom wall separating portion 34 is further formed between the tray 20 and the bottom wall 12. The bottom wall separation part 34 becomes a flow path for cold air, gas, water, etc. together with the side wall separation part 31 and the bottom plate corner through-hole 25, so that the above effect is more remarkably exhibited.

Further, in the live fish transport container 1 of the present embodiment,

notches

24a ₁ , 24a ₂ , 24b ₁ , 24b ₂ are formed in the side plates 24 on both sides in the width direction of the tray 20, and the side plates 24 are partially lowered. Yes. Also by this, the cool air or the gas to be filled is easily passed through the entire live fish transport container 1. In particular, when the plurality of trays 20 are stacked in the height direction, the side plate 24 of the tray 20 is partially lowered, so that the cooler is filled or cooled between the adjacent trays 20 in the height direction. Gas is easy to flow through. Further, in the water draining process in the method for transporting anhydrous live fish according to the present invention, the

notches

24a ₁ , 24a ₂ , 24b ₁ , 24b ₂ become water flow paths when water is discharged from the live fish transport container 1. Easily drain water.

Further, in the live fish transport container 1 of the present embodiment, a recess 28 is formed in the bottom plate 23 of the tray 20. Thereby, while the live fish F is mounted on the bottom plate 23, the load of the own weight added to the live fish F is reduced, and the survival rate of the live fish F in anhydrous transport is further increased.

Also, in the live fish transport container 1 of the present embodiment, one live fish is placed on one tray 20. Therefore, live fish F do not contact each other. Thereby, the fish surface is not damaged, and in addition, there is no possibility that the fish in a low breathing state (a state where the motion of the gills or the heart rate is lowered) will be awakened by the contact and will die in the anhydrous state.

Although not shown, two trays 60 can be used instead of the four trays 20 used in FIG. That is, the packaging body 100 can be configured by stacking the trays 60 on which two live fish F are placed in two stages with the tail mounting portions 62 facing in the same direction, and the same effect is achieved. Further, the bottom plate corner through-hole 25 in the tray 20 is not formed in the tray 60. However, since the tray 60 has a portion in which the side plate 64 is not formed on the periphery of the bottom plate 63, cold air, gas, water, etc. It becomes a flow path and has the same effect.

Each tray 20 provided in the live fish transport container 1 of the present embodiment can be used alone as a live fish transport tray. In this specification, in particular, the tray 20 when used as a live fish transport tray may be displayed as a “live fish transport tray 20”. When the live fish transport tray 20 is used as the live fish transport member, a sheet such as a buffer sheet may be interposed between the live fish transport tray 20 and the live fish F.

<Livestock raising process>
The livestock raising process is a process of raising fish in water at a temperature within the first temperature range R1.

In this specification, all temperatures are expressed in units of Celsius (° C).

Livestock raising process may be carried out in an aquarium, live fish transportation member, or a section installed by being surrounded by a fence such as a net or fence in the sea, pond, river, etc. Although it may be performed in, it is preferable to perform in a water tank or a member for transporting live fish. Moreover, it is not necessary to carry out the livestock raising process in only one place, but in a plurality of places (for example, in the water tank at the start of the livestock raising process and in the live fish transport member from the middle, the livestock raising process is performed in the multiple water tanks and live fish transport member Etc. in order).

When performing the animal husbandry process in the aquarium, the aquarium to be used is not particularly limited with respect to size, shape, installation location, and the like. For example, the livestock raising process can be performed in water tanks installed in various places such as a ship, a fish market, a farm, a food processing facility, etc., or can be sequentially performed in a plurality of water tanks. The water tank only needs to be able to contain water, and a relatively small size called a container is also included in the water tank.

When the live fish transport member is a live fish transport tray, the livestock raising process is performed in the live fish transport tray. Specifically, the live fish is placed on the live fish transport tray installed in the water, It refers to performing livestock raising processes. Specifically, as shown in FIG. 15, the water W is accommodated in another container (aquarium or the like) 100, and the trays 20 on which the live fish F are placed are stacked in multiple stages and installed in the water W. In this state, the breeding process is performed using the water temperature of the water W as the temperature in the first temperature range R1. In the illustrated embodiment, the tray 20 on which the live fish F is placed is placed on the tray 20 on which the weights 200 are stacked so as not to float in the water W.

The water temperature at the time of the animal husbandry process is not particularly limited as long as the temperature is not less than the lower limit temperature R1L of the first temperature range R1 and not more than the upper limit temperature R1U. The water temperature during the breeding process is not necessarily the same during the period, and may vary continuously or stepwise within the range of R1.

The livestock raising process has the role of resting fish that have been stressed by catching or moving.

It is preferable that the lower limit temperature R1L and the upper limit temperature R1U of the first temperature range R1 are both in the range of 8 to 30 ° C. The lower limit temperature R1L is preferably 9 ° C, more preferably 10 ° C. The upper limit temperature R1U is preferably 25 ° C, more preferably 20 ° C, and more preferably 15 ° C. This temperature range is a temperature range suitable for inducing a resting state in which the physiological metabolic function and spontaneous movement of fish are suppressed and the vitality is reduced, particularly in the fish of the flounder family.

The first temperature range R1 is preferably a temperature range in which a physiological metabolic function and spontaneous movement of fish are suppressed and a resting state in which vitality is reduced can be induced. Such temperature can be set according to the temperature of the water at the time of fish catch (whether natural or aquaculture). As shown in the preliminary test described later, when the water temperature at the time of fishing is high, the fish is in a relatively high water temperature and the state where the movement frequency of the trout is low, that is, a calm state, but the water temperature at the time of fishing is low. In some cases, fish reach a resting state at relatively low water temperatures. Therefore, for each fish, a correlation between the water temperature at the time of fishing and the water temperature for inducing a resting state is obtained in advance, and based on the correlation, the first temperature range is set according to the temperature of the water at the time of fishing of the fish. It is preferable to perform a water temperature setting step for setting the upper limit temperature and the lower limit temperature of R1.

The time for the animal husbandry process is not particularly limited, but is usually 5 hours or more, preferably 10 hours or more, and usually 96 hours or less, preferably 72 hours or less.

In particular, a predetermined threshold temperature P in the first temperature range R1 (where P is a temperature of 8 to 15 ° C., preferably 15 ° C., 14 ° C., 13 ° C., 12 ° C., 11 ° C., 10 ° C., 9 ° C. or 8 ° C., more preferably 13 ° C., 12 ° C. or 11 ° C., particularly preferably 12 ° C.) When fish are bred in water at a temperature below, the physiological metabolism of fish in fish The function and the spontaneous movement can be suppressed, and a resting state in which the vitality is reduced can be particularly appropriately induced, and the subsequent process (for example, the process after the acclimation process or the process after the cooling process when including the cooling process) This reduces the possibility of stressing fish. In order to exhibit this effect, it is preferable to perform the animal husbandry process in water at a temperature equal to or lower than the threshold temperature P at the last stage of the animal breeding process. The animal husbandry process performed in water having a temperature equal to or lower than the threshold temperature P may be referred to as a “rest animal breeding process” in the present invention. As can be seen from a comparison between Example 2 and Example 3 to be described later, when performing a resting feeding process at a temperature equal to or lower than the threshold temperature P in the method of the present invention, the fish meat obtained from live fish after transportation is stressed. (The white fish meat does not have a red color), and as a result, there is little raw odor and a suitable effect that the body is tightened and has a moderately hard texture. The lower limit of the temperature in the resting cultivation process is not particularly limited as long as it is not lower than the lower limit temperature R1L, and specifically, may be 8 ° C. or higher. The water temperature in the rest livestock raising process (livestock raising process 2 to be described later) may be R1L or more and the threshold temperature P or less. Usually, when the temperature P is P (° C), (P-2) ° C or more, P Or less, more preferably (P-1) ° C. or more and P or less, and most preferably P. The time for the resting cultivation process is not particularly limited, but the fish is preferably 5 hours or more, more preferably 10 hours or more, preferably 48 hours or less, more preferably 36 hours or less, more preferably in water having a temperature equal to or lower than the threshold temperature P. When the animal is rested for 24 hours or less, the above-mentioned preferable effects are remarkably exhibited.

The rest breeding process may be performed in a water tank or a live fish transport member. In the resting rearing process, fish may be stressed and mucus may be secreted from the body surface into the surrounding water, but the resting rearing process is performed in the aquarium and the subsequent acclimation process or cooling process contains new water If it is performed in the member for transporting live fish, it is easy to separate the fish from the water containing the mucus produced in the resting breeding process and perform the acclimation process or the cooling process in fresh water. On the other hand, if the fish is cooled in the rest livestock rearing process and the fish is transferred from the aquarium to another aquarium or container, the fish is stressed, but if the rest livestock rearing process is performed in the live fish transportation member, The process can be continued in the same member and is preferable because stress applied to fish is reduced.

The water temperature during the animal husbandry process is not necessarily the same during the period, and may vary continuously or stepwise. For example, the livestock raising process may be performed at a water temperature close to the temperature of water at the time of catching fish (whether natural or aquaculture), and then the rest livestock raising process may be performed. Water temperature close to the water temperature at the time of fishing of fish (for example, when the water temperature at the time of fishing is T _R (° C.), it is within the range of (T _R +2) ° C. to (T _R −2) ° C., preferably (T _R +1) ° C. The livestock raising process performed in the range of (T _R −1) ° C.) may be referred to as “cured livestock raising process”. In the curing and raising process, the stress given to the fish is small because the breeding is carried out at a water temperature close to the water temperature at the time of fish catch. Specifically, in the present invention, the livestock raising process is the first temperature range after the livestock raising process 1 and the livestock raising process 1 in which the fish is stored in water having a temperature exceeding the temperature P in the first temperature range R1. It is preferable to include a livestock raising step 2 for raising fish in water having a temperature equal to or lower than the threshold temperature P in R1. In addition, when the water temperature at the time of fishing of fish is the threshold temperature P or less, it is not necessary to perform a curing livestock raising process separately, and you may perform a rest livestock raising process from the beginning. Advantages of combining the resting and rearing process and the curing and rearing process that is performed as necessary before that are as follows. That is, in general, fish such as flounder are often caught at a water temperature in the range up to 30 ° C., and the resting and raising process is performed at a temperature equal to or lower than the temperature P from the start of the raising process regardless of the water temperature at the time of fishing. It is inevitable that the fish caught at high water temperature exceeding the temperature P will be greatly stressed. Therefore, if the above-mentioned curing and raising process is performed according to the water temperature at the time of fishing, and then the resting and raising process is performed, the resting and raising process is performed while suppressing the stress applied to fish from a wide temperature range. Is possible. It should be noted that the water temperature is not necessarily the same during the period in the curing and breeding process (stock raising process 1), and may vary continuously or stepwise. For example, during the period of the curing and breeding process (stock raising process 1), The water temperature can be lowered continuously or stepwise so as to approach the temperature P over time. The time for the curing and breeding process is not particularly limited, but the fish is preferably cultivated for 5 hours or more, more preferably 10 hours or more, preferably 48 hours or less, more preferably 36 hours or less in water at a temperature exceeding the temperature P. Then, when the above-described resting and raising process is performed, the above-described preferable effect is remarkably exhibited. The water temperature in the curing and raising process (stock raising process 1) is higher than the threshold temperature P and may be equal to or lower than the upper limit temperature R1U of the first temperature range R1, but normally when the temperature P is set to P (° C.), (P + 0.5) ° C. or higher and R1U or lower, more preferably (P + 1) ° C. or higher and R1U or lower, more preferably (P + 2) ° C. or higher and R1U or lower.

In the present invention, farmed process, a farming step 1 of farmed fish in water at a temperature T _A in the first temperature range R1, after farmed step 1, in a first temperature range R1, the temperature T preferably includes a farming step 2 of farmed fish in water lower than _a temperature T _B. Here, the temperature T _A is the temperature above the threshold temperature P, when the temperature T _B is the threshold temperature P below temperature is preferable because the effect of the paragraph are achieved, limited to this temperature range Not. By performing the animal husbandry process while lowering the water temperature in at least two stages, it suppresses the physiological metabolic function and spontaneous movement of the fish in fish, particularly in the flounder family, and induces a resting state with reduced vitality particularly appropriately It becomes easier to reduce the possibility of stressing the fish in subsequent steps. Temperature T _B at the temperature T _A and farming Step 2 in farming Step 1, respectively, not necessarily the same during the period may be varied continuously or stepwise. Livestock raising step 1 can be performed for 5 hours or longer, more preferably 10 hours or longer, preferably 48 hours or shorter, more preferably 36 hours or shorter, and livestock step 2 is preferably 5 hours or longer, more preferably It can be performed for 10 hours or longer, preferably 48 hours or shorter, more preferably 36 hours or shorter, more preferably 24 hours or shorter. When the livestock raising process includes the livestock raising process 1 and the livestock raising process 2, it is preferable to perform them in different places (for example, in different water tanks). When performing farming Step 1 and farmed step 2 and at separate locations, after the completion of farmed step 1, the live fish, it is preferable to start the farming step 2 is moved in the water temperature T _B. In this embodiment, different temperature of water may be prepared two aquarium or live fish transport member containing a, a special device as compared to the embodiment of changing the temperature T _B the temperature from the temperature T _A in a single water tank do not need.

Alternatively, if the farmed step comprises a farming step 1 and farmed step 2 performs respective elsewhere (e.g. in a separate water tank), after the completion of farmed step 1, live fish, and the temperature higher than the temperature T _B moving the water, then the temperature of the water is cooled to a temperature T _B, then, it is also possible to start the farmed step 2. Here, the above high temperature of the water than the temperature T _B is preferably a high and the temperature T _A less water than the temperature T _B, more preferably water having a temperature T _A.

In farming process, it is usually a temperature difference between the temperature T _A and the temperature T _B is 0.5 ℃ higher, for example at 1 ℃ or more.

In farming step in addition to the farming step 2 performed in water at farming Step 1 and the temperature T _B performed in water at a temperature T _A, it may further comprise the step of farmed fish in water at other temperatures, the water in the farming process The number of times of changing the temperature is preferably up to 3, preferably 1 or 2, and particularly preferably 1. When the number of times is 1, the animal husbandry process includes only the animal husbandry process 1 and the animal breeding process 2. The time or cost required for the animal husbandry process can be reduced as the number of times decreases.

<Acclimation process, draining process, anhydrous transport process>
The present invention is characterized in that the acclimatization process, draining process and anhydrous transport process for fish after the livestock breeding process are performed in a state of being housed in a live fish transport member. There is no need to transfer fish between the acclimatization process, draining process and anhydrous transport process. Hereinafter, each step will be described.

<Adaptation process>
The acclimatization step is a step of acclimatizing fish in a live fish transporting member containing water having a temperature within the second temperature range R2.

The temperature is not particularly limited as long as it is a temperature not lower than the lower limit temperature R2L and not higher than the upper limit temperature R2U of the second temperature range R2. The water temperature during the acclimation process is not necessarily the same during the period and may vary continuously or stepwise within the range of R2. The upper limit temperature R2U is set to a temperature lower than the lower limit temperature R1L of the first temperature range R1.

The acclimatization process is a process of inducing hypopnea state by acclimatizing fish in water at a temperature lower than that of the animal breeding process. The hypopnea state refers to a state in which the exercise frequency and heart rate of the heel are reduced. For example, the exercise frequency of the heel is 50% or less, preferably 40% or less, more preferably 30 as compared to the water temperature at the time of fishing. % Or less, more preferably 25% or less, more preferably 20% or less can be referred to as a “hypopnea state”. The lower limit of the exercise frequency of the carp is not particularly limited, but is usually 5% or more, preferably 10% or more, and more preferably 15% or more compared to during the water temperature at the time of fishing. The movement frequency of the fish represents the number of times of fish fish exercise measured using a piezoelectric element or by visual observation as a number of times per unit time (for example, 1 minute). In the case of flounder fish, it is determined that the state of hypopnea is in the state where the exercise frequency of the shark is preferably 2 to 20 times, more preferably 5 times or more, more preferably 15 times or less per minute. Can do. The second temperature range R2 of the water temperature for inducing a hypopnea state can be easily obtained through experiments for each species of fish.

It is preferable that the lower limit temperature R2L and the upper limit temperature R2U of the second temperature range R2 are both in the range of −2 to + 7 ° C. The lower limit temperature R2L is preferably 0 ° C, more preferably 2 ° C. Maximum temperature R2U is preferably + 6 ° C, more preferably + 5 ° C. This temperature range is a suitable temperature range for inducing a hypopnea state, particularly in flounder fish.

The second temperature range R2 can be set according to the temperature of water at the time of fish catching (whether natural or aquaculture). As shown in the preliminary test described later, when the water temperature at the time of fishing is high, the fish reaches a low breathing state at a relatively high water temperature, but when the water temperature at the time of fishing is low, the fish is at a relatively low water temperature. It leads to hypopnea. Therefore, for each fish, a correlation between the water temperature at the time of fishing and the water temperature that induces the hypopnea state is obtained in advance, and based on the correlation, the second temperature is determined according to the temperature of the water at the time of fish fishing. It is preferable to perform a water temperature setting step for setting the upper limit temperature and the lower limit temperature of the region R2.

The time for the acclimation process is not particularly limited, but is usually 1 to 24 hours, preferably 2 hours or more, preferably 18 hours or less.

The acclimatization process is performed in a live fish transporting member containing the fish and water after the livestock raising process, and is preferably performed while supplying air or oxygen into the water in the live fish transporting member during the acclimatization process.

<Draining process>
The water draining step is a step performed after the acclimatization step, and is a step of draining water from the live fish transporting member to make the inside of the live fish transporting member anhydrous.

Means for draining water from the live fish transporting member is not particularly limited, but preferably, when the live fish transporting member is a live fish transporting container as described above, the container is formed from a through hole or a drain provided in the live fish transporting container. Drain the water inside.

It is preferable to close the through hole or the drain port after the water draining step.
The water draining process is not limited to the above-described embodiment, as long as it is a process of separating live fish accommodated in the live fish transporting member from the water while being accommodated in the live fish transporting member. For example, as shown in FIG. 15, a state in which the water W is accommodated in another container (such as an aquarium) 100 and the live fish transport trays 20 on which the live fish F are placed are stacked in multiple stages and immersed in the water W. When performing the acclimatization process of the present invention, lifting the live fish transport tray 20 containing the live fish F from the water W after the acclimatization process is also an aspect of the draining process.

<Anhydrous transport process>
The anhydrous transport step is a step of transporting fish while keeping them live in the live fish transport member.

The environment in the live fish transport member in the transport process is not particularly limited as long as the fish induced in the low breathing state in the acclimatization process can survive anhydrous. Here, the environment in the live fish transport member (the environment specified by various indicators such as the temperature of the atmosphere, the oxygen concentration in the atmosphere, etc.) refers to the inside of the container when the live fish transport member is a live fish transport container. When the member for transporting live fish is a tray for transporting live fish, it indicates the environment around the live fish placed on the tray. When the live fish transport member is a live fish transport tray, in the anhydrous transport process, the live fish transport tray is usually housed in a container for storage or a cool box of a cold car and transported. The environment in the storage container or the cold storage corresponds to the “environment in the live fish transport tray”. The temperature of the atmosphere in the live fish transport member is not particularly limited, but is preferably a temperature within the range of the second temperature range R2 in order to maintain a low respiratory state. There is no problem even if the temperature deviates from the second temperature range R2 during transportation, but the upper limit of the temperature of the atmosphere in the live fish transporting member during transportation may exceed the upper limit temperature R2U of the second temperature range R2 by 5 ° C. Preferably, it is less than (R2U + 5) ° C, more preferably not more than 3 ° C, ie less than (R2U + 3) ° C. Similarly, the lower limit of the temperature of the atmosphere in the live fish transporting member during transportation is not lower than the lower limit temperature R2L of the second temperature range R2 by 5 ° C., that is, higher than (R2L-5) ° C. Preferably, it is not lower than 3 ° C., that is, higher than (R2L-3) ° C.

In the anhydrous transport process, for example, a container having the same structure as the live fish transport container 1 described with reference to FIGS. 1 to 3 can be suitably used as a container for housing the live fish transport tray.

When the live fish transporting member is a live fish transporting container, in order to keep the temperature of the atmosphere in the live fish transporting container during the anhydrous transporting process as low as possible, a cryogen in the housing space of the live fish transporting container Is preferably arranged. The temperature of the cryogen is usually in the range of -5 to 5 ° C. Further, the amount of the cryogen can be appropriately adjusted according to the capacity of the storage space for the live fish transport container, but preferably 0.3 kg or more when the capacity of the storage space for the live fish transport container is 5 to 100 L. , Preferably 2.0 kg or less, more preferably 0.5 kg or more, and more preferably 1.0 kg or less. In the winter season, there may be a case where no cryogen is used. Examples of the cooling agent include ice, dry ice, and water-containing gel of elastic polymer of acrylamide or methacrylamide and its crosslinkable monomer (N, N-methylenebisacrylamide or N, N-methylenebismethacrylamide). It can be configured using.

Further, in the anhydrous transport process, the oxygen concentration in the atmosphere in the live fish transport member is preferably maintained at 35% by volume or more, preferably 40% by volume or more, and 60% by volume through the process. It is more preferably maintained above, more preferably 70% by volume or more, and particularly preferably 80% by volume or more. The oxygen gas concentration in the live fish transport member can be measured by using an oxygen concentration measuring device and inserting the tip of the oxygen detector into the accommodation space. The oxygen gas concentration at the start of the anhydrous transport process in the atmosphere in the live fish transporting member is preferably 35% by volume or more, more preferably 40% by volume or more, more preferably 60% by volume or more, more preferably 70% by volume or more. Particularly preferably, the volume is 80% by volume or more, and when the live fish transporting member is a live fish transporting container, the atmosphere inside the container is not substantially leaked outside the container, and the live fish transporting member is the live fish transporting tray. In the case where the live fish transport tray is housed in a container for storage or a refrigerator in a cold car and transported, if the atmosphere inside the container for storage or the refrigerator is not substantially leaked to the outside, The oxygen concentration in the atmosphere in the live fish transport member can be maintained within the above range throughout the anhydrous transport process, and oxygen necessary for survival is supplied to the fish.

The time for the anhydrous transport step is not particularly limited, but is usually 1 to 48 hours, preferably 5 hours or more, and preferably 30 hours or less.

In addition, when the live fish transport member is a live fish transport container, during the anhydrous transport process, the container body of the live fish transport container is covered with a lid and the inside of the container is kept in a dark state. Can be lowered, which is preferable.

<Operation to move from the animal husbandry process to the acclimatization process>
As described above, the breeding process is a process of raising fish in water at a temperature in the first temperature range R1, and the acclimation process performed thereafter is water at a temperature in the lower temperature range R2. Is a process of acclimatizing fish in a member for transporting live fish.

The operation when shifting from the animal husbandry process to the acclimatization process is not particularly limited.

The simplest operation to move from the nurturing process to the acclimatization process is the operation in the embodiment whose temperature diagram is shown in FIG. In this embodiment, between the time t ₀ to t _1, performs a farming step in water at a temperature T ₀ in the first temperature range R1, then at time t _1, the fish, the water farmed is performed Then, the acclimatization process is started by moving to a member for transporting live fish containing water having a temperature T2 in the _second temperature range R2. Conditioned step is carried out from time _{t 1} to _{t 2.} It performs drainage process at time _{t 2, the} performing anhydrous transportation step from time _{t 2} to _{t 3.} Note the range of the temperature T ₄ in the members of anhydrous transportation step is as described for anhydrous transportation step, may be different may be the same as the T ₂ of the with conditioned process. If T ₂ and T ₄ are different, either may be relatively high.

<Cooling process>
As a more preferable embodiment which shifts to the acclimatization process from an animal husbandry process, it is an embodiment which passes through the cooling process which cools fish in water during the middle or the end of an animal breeding process to the start or middle of an acclimatization process. By performing the cooling process, it is possible to reduce the stress due to temperature changes given to fish. Hereinafter, this cooling process will be described in detail.

The temperature change rate (cooling rate) in the cooling step is not particularly limited, but preferably the fish is in water, the water temperature is preferably −0.1 to −10 ° C./hour, more preferably −0.2 ° C./hour or more. (Indicates that the absolute value of the cooling rate is −0.2 ° C./hour or higher. The same applies hereinafter), more preferably −9 ° C./hour or lower (−9 ° C./hour or lower cooling rate) The cooling is carried out by changing at a rate of −0.3 ° C./hour or more, more preferably −8 ° C./hour or less. The cooling rate here is an average cooling rate obtained by dividing the difference (° C.) obtained by subtracting the water temperature at the start of the cooling process from the water temperature at the end of the cooling process by the time (hours) required for the cooling process. is there. However, the instantaneous cooling rate at each point in the course of the cooling process is more preferably within the above range. When the cooling rate is within the above range, it is possible to further reduce the stress due to temperature changes given to fish.

The cooling rate may be constant from the start to the end of the cooling process, or may change continuously or stepwise. More preferably, the absolute value of the change rate of the water temperature continuously decreases with time. To cool. Such a cooling process is considered to further reduce the stress applied to fish. Such a cooling process can be realized by the following configuration. That is, when the target temperature of the cooling process is the temperature T ₂ (° C.) in the second temperature range R2, heat insulation containing water at a temperature higher than T ₂ (for example, T ₁ or T ₃ described later). The fish is put in a container having a characteristic, and the temperature inside the container is lower than the temperature in the second temperature range R2, for example, T ₂ or T ₂ (for example, less than T ₂ and (T ₂ −0. 5) Temperature from 0 ° C. to (T ₂ −40) ° C., preferably less than T _{2 and} temperature from (T ₂ −0.5) ° C. to (T ₂ −25) ° C., more preferably less than T ₂ And (T ₂ −0.5) ° C. to (T ₂ −15) ° C., more preferably less than T ₂ and (T ₂ −0.5) ° C. to (T ₂ −5) ° C. Leave it in the refrigerator compartment. As time passes, the water temperature in the container gradually approaches T _2, and the water in the container at this time has an absolute value of the change rate of the water temperature continuously with time as can be seen from the result of Example 1 in FIG. It is cooled down to This cooling process uses a normal refrigerator capable of maintaining the inside temperature at a constant temperature and a general heat insulating container (for example, a container made of a plastic material having heat insulating properties, preferably a foamed plastic). This is advantageous because it does not require a generally expensive refrigerator or water tank that can control the rate of temperature change as in Patent Document 1 and Non-Patent Document 1. In this case, the heat insulating container is particularly preferably a heat insulating live fish container.

When performing the cooling process in the live fish transport member, after moving the fish from the place where the livestock breeding process has been performed to the live fish transport member that contains water in the housing space, during or after the livestock breeding process. A cooling process is performed. In the case of this movement, when the live fish transport member is a live fish transport container, it is preferable that the accommodation space of the live fish transport container is filled with water in advance and the fish is immersed while discharging excess water.

When the cooling process is performed in the live fish transporting member, air or oxygen is appropriately added to the water in the live fish transporting member continuously or intermittently at an appropriate time from the start of the cooling process to the end of the acclimatization process. Is preferably supplied.
When the live fish transporting member is a live fish transporting tray, as shown in FIG. 15, in the water W accommodated in another container 100, the live fish transporting tray 20 on which the live fish F is placed is immersed, The cooling step can be performed by cooling the water W with a chiller (not shown) or the like. At this time, air or oxygen can be appropriately supplied to the water W in the live fish transport tray 20 (in the other container 100) continuously or intermittently.

In addition, when the live fish transport member is a live fish transport container and the cooling process is performed in the live fish transport container, the container body is covered with a lid and the inside of the container is kept in a dark state. Since it can reduce, it is preferable.

A preferred embodiment of the cooling step will be described with reference to temperature diagrams shown in FIGS.

<Preferred Embodiment 1 of Cooling Step (FIG. 7)>
In the embodiment shown in FIG. 7 performed in water at a temperature T ₀ of the inside first temperature range R1 from the time t ₀ at the beginning of the farming process until t _1. Followed by a moving step of moving the fish for live fish transport member in accommodating the water temperature T ₁ of in the first temperature range R1 at time t _1. In FIG. 7, for the sake of illustration, T ₀ and T ₁ are depicted as if they are different temperatures, but T ₀ and T ₁ may be the same temperature or different temperatures. . If T ₀ and T ₁ are different, either may be relatively high.

Next, after the end of the moving process (that is, at time t ₁ or later), a cooling process is performed to cool the temperature of the water in the live fish transporting member to the temperature T ₂ in the second temperature range R2. . In the embodiment shown in FIG. 7, from time t ₁ to t _2, after holding the temperature in the member to a temperature T _1, an example of performing a cooling step from the time t ₂ to t _5. After the moving process at time t ₁ , the cooling process may be performed immediately without keeping the water temperature in the member at the temperature T ₁ (in this case, t ₁ = t ₂ ).

Water temperature in a live fish transport member in a cooling process leads to temperature T ₂ gradually decreases. Farming process, because a step of farmed fish in the first temperature range R1, from time t ₀ which began farming process, the time t the water temperature in the cooling step is the lower limit temperature R1L the first temperature range R1 Up to ₃ corresponds to the animal husbandry process.

The acclimatization step is a step of acclimatizing fish in a live fish transporting member containing water having a temperature within the second temperature range R2. Thus, in this embodiment, from the time t ₄ when the water temperature in the cooling step is the upper limit temperature R2U the second temperature range R2, until time t ₆ for performing drainage process, corresponds to the conditioned process.

The cooling step is performed from time _{t 2} to _{t 5.} In the illustrated embodiment, in the cooling step, it becomes substantially zero at the time t ₅ the absolute value of the water temperature change rate is continuously decreased over time. In order to realize this, a heat-insulating live fish transport container and a temperature lower than T ₂ or T ₂ (for example, less than T ₂ and (T ₂ −0.5) ° C. to (T ₂ Temperatures up to −40) ° C., preferably below T _{2 and} temperatures from (T ₂ −0.5) ° C. to (T ₂ −25) ° C., more preferably below T ₂ and (T ₂ − 0.5) ° C. to (T ₂ −15) ° C., more preferably (T ₂ −0.5) ° C. to (T ₂ −5) ° C.). It is as follows. Further, as described above, the change in the cooling rate is not limited to this form.

It performs drainage process at time _{t 6,} performs anhydrous transportation step from time _{t 6} to _{t 7.} Note the range of the temperature T ₄ in the members of anhydrous transportation step is as described for anhydrous transportation step, may be different may be the same as the T ₂ of the with conditioned process.

<Preferred Embodiment 2 of Cooling Step (FIG. 8)>
In the embodiment shown in FIG. 8, the animal husbandry process is performed in water at a temperature T ₀ within the first temperature range R 1 from time t ₀ to t ₁ . Then move the fish during the first live fish transport member containing a water high temperature T ₃ than the temperature T ₂ of the and in the second temperature range R2 lower than the lower limit temperature R1L temperature range R1 at time t ₁ The moving process is performed. In this embodiment, farming process is terminated at time t ₁ is started at time t _0.

Then, (ie in the time t ₁ or time after it than) closes the moving step, the cooling step of cooling the temperature of the water in a live fish transport member to a temperature T _2.

In this cooling step, the water temperature in the live fish transport member gradually decreases and reaches the temperature T ₂ at time t ₃ .

The acclimatization step is a step of acclimatizing fish in a live fish transporting member containing water having a temperature within the second temperature range R2. Thus, in this embodiment, from the time t ₂ when the water temperature is the upper limit temperature R2U the second temperature range R2 in the cooling step, until the time t ₄ when performing drainage process, corresponds to the conditioned process.

Cooling step is carried out from time _{t 1} to _{t 3.} In the illustrated embodiment, in the cooling step, it becomes substantially zero at time t ₃ the absolute value of the water temperature change rate is continuously decreased over time. In order to realize this, a heat-insulating live fish transport container and a temperature lower than T ₂ or T ₂ (for example, less than T ₂ and (T ₂ −0.5) ° C. to (T ₂ Temperatures up to −40) ° C., preferably below T _{2 and} temperatures from (T ₂ −0.5) ° C. to (T ₂ −25) ° C., more preferably below T ₂ and (T ₂ − 0.5) ° C. to (T ₂ −15) ° C., more preferably (T ₂ −0.5) ° C. to (T ₂ −5) ° C.). It is as follows. Further, as described above, the change in the cooling rate is not limited to this form.

It performs drainage process at time _{t 4,} performs anhydrous transportation step from time _{t 4} to _{t 5.} Note the range of the temperature T ₄ of the live fish transport within member anhydrous transportation step is as described for anhydrous transportation step, may be different may be the same as the T ₂ of the with conditioned process. If T ₂ and T ₄ are different, either may be relatively high.

<Embodiment (FIG. 9) which combined a curing livestock breeding process, a rest livestock breeding process, and a cooling process>
The embodiment shown in FIG. 9 is a modified example in which the livestock raising process is performed in two stages including the cured livestock raising process and the rest livestock raising process in the embodiment shown in FIG.

Introduction The farming process from time t ₀ to t ₁ performed in water at a temperature T _A in the first temperature range R1. Here the temperature T _A is the temperature higher than the threshold temperature P above, farming step from time t ₀ to t ₁ corresponds to curing farming process. Followed by a farming subsequently at time t ₁ in water at a temperature T _B in the first temperature range R1. In this case the temperature T _B is the threshold temperature P temperatures below farming process from time t ₁ to t ₂ corresponds to rest farming process. At this time, R1L is preferably 8 ° C. Specific examples of the threshold temperature P are as described above, and when the fish is a fish belonging to the flounder family, it is preferably 12 ° C.

Live fish timing of transport moving step for moving to members in is not particularly limited, and the moving step the rest farmed process later performed at time t ₁ may be carried out in a live fish transport member in, at time t ₂ the subsequent cooling step may be performed in a member for live fish transport performing moving step, all steps from time t ₀ is the start of the curing farmed step may be carried out in a live fish transport member in.

Since the embodiment shown in FIG. 9 is the same as that described in the embodiment shown in FIG.

<Method for inducing hypopnea in live fish>
Another aspect of the invention relates to a method for inducing a hypopnea condition in live fish. The method includes the above farming step comprises at least a habituation process, the farming process, a farming step 1 of farmed fish in water of the first temperature region of the temperature T _A, after the farmed step 1, said first temperature range, characterized in that it comprises at least a farming step 2 of farmed fish in water at low temperature T _B than the temperature T _a.

In this aspect of the present invention, a farming step 1 of farmed fish in water at a temperature T _A as farmed step performed prior to the habituation process, farming step of farmed fish in water at low temperature T _B than the temperature T _A 2 is able to appropriately induce a resting state in which physiological metabolic functions and spontaneous movements are suppressed and vitality is reduced in fish, so that the fish may be stressed in the subsequent acclimation process. It is possible to induce a hypopnea state while reducing. As a result, the stress received by live fish can be reduced, and the possibility that the quality of live fish will be impaired can be reduced.

The farming process, is preferably a temperature at which the temperature T _A exceeds a threshold temperature P, the temperature T _B is less temperature threshold temperature P, temperature threshold temperature P is in the range of 8 ° C. or higher 15 ℃ less . The livestock raising process under this condition is particularly preferable for the purpose of inducing a resting state in which physiological metabolic functions and spontaneous movement are suppressed and vitality is reduced in fish belonging to the family Flounder, particularly in the active fish of flounder. Preferred embodiments of the temperature T _A , the temperature T _B , and the threshold temperature P are as described above.

When the acclimatization step is performed while supplying air or oxygen into water, it is preferable because the survival rate of live fish induced in a low respiratory state is increased.

In this aspect, it is preferable to perform a cooling step in which the fish is cooled in water while the water temperature is continuously reduced during or after the livestock raising step until the start or halfway of the acclimatization step. In the cooling process, by continuously changing the water temperature, it is possible to further reduce the stress applied to the fish as compared with the case where the water temperature is changed step by step.

The livestock raising process and the acclimatization process in this aspect, and the cooling process and the water temperature setting process performed as necessary are the same as the respective processes in the live fish transportation method of the present invention, and thus the description thereof is omitted.

Hereinafter, the present invention will be described in more detail with reference to examples and comparative examples.
In the following examples and comparative examples, the live fish transport container 1 was used as a live fish transport member.

Example 1
<Induction of flounder to hypopnea>
“Hypnea state” refers to a state in which Ella's exercise or heart rate has decreased.

A first water tank (size: length 1 m × width 1 m × height 0.5 m) prepared at a constant seawater temperature at a breeding temperature was prepared.

Further, a live fish transporting container 1 made of a molded polystyrene foam resin body having a container body 10 and a lid body 50 of the following size was prepared in the same manner as shown in FIG. In order to accommodate the live fish F, a tray 20 having the same form as shown in FIG. 2 was used, and the following buffer sheet S was installed on the bottom plate 23 thereof.

Container body 10: width 484 mm × length 525 mm × height 160 mm, side wall 13 thickness 18 mm, bottom wall 12 thickness 20 mm (container dimensions: width 448 mm × length 489 mm × height 140 mm, capacity: 30 L), expansion ratio 50 times (density 0.02 g / cm ³ ).

Lid 50: width 484 mm × length 525 mm × thickness 24 mm, expansion ratio 50 times (density 0.02 g / cm ³ ).

Tray 20: The thickness of the bottom plate 23 is 15 mm, the width of the body placing portion 21 is 219 mm × the length of 363 mm, the width of the tail placement portion 22 is 101 mm × the length of 116 mm, and the expansion ratio is 50 times (density 0.02 g / cm ³ ).

Buffer sheet S: A polyurethane resin foam sheet is used. Thickness 5mm.

The seawater temperature in the first tank was 10 ° C., and 4 flounder (about 1 kg / animal) were bred for 24 hours.

Next, 30 liters of seawater from the first tank was put into the housing space of the container body 10.

Next, as shown in FIG. 3 (however, seawater is not shown in FIG. 3), the four trays 20 on which the flounder F is placed via the buffer sheet S are filled with 10 ° C. seawater. Within the 10 accommodation spaces, the tail placement portions 22 were arranged in two rows and two rows in the same direction and submerged. At this time, surplus seawater overflowed out of the container and was discharged. Next, the opening of the container body 10 was closed with the lid 50. The lid 50 was closed so that the lid 50 did not come off the container body 10 due to the buoyancy of the tray 20.

Live fish transporting the tip of the tube with the spout for introducing air into the seawater in the live fish transport container 1 through the lid through hole 51 of the lid 50 (the lid through hole formed near the tail of the flounder F) The aeration pump was set outside the container at the other end of the tube. While supplying air to the seawater in the live fish transport container 1 using the pump, it was left in a refrigerator set at 3 ° C. for 24 hours.

After 24 hours, the seawater temperature in the container was 4 ° C., and it was confirmed that flounder F was induced in a hypopnea state (a state where the motion of the gills and the heart rate were reduced).

The change in seawater temperature in the container with respect to the elapsed cooling time is shown in FIG. In FIG. 4, the seawater temperature was about 4 ° C. during the cooling elapsed time of 16 to 24 hours, and it was confirmed that the seawater was acclimatized for 8 hours.

Next, the tube that introduced air into the seawater in the live fish transport container 1 was removed, a part of the thin part 11 formed on the side wall 13 of the container body 10 was cut from the outside with a cutter to form a through hole, The stored seawater was drained through the through hole. A part of the cut-out thin portion 11 was taken out of the container body 10 by the pressure of seawater. After draining, the through hole was closed with an adhesive tape. Thereby, air flows into the side wall separation portion 31 between the tray 20 and the side wall 13 of the container body 10 and the separation portion (not shown) between the tray 20 and the bottom wall 12 of the container body 10, In the vicinity of the tail placement portion 22, the cold

insulation storage portions

32 and 33 are formed.

Next, the lid body 50 was removed, 0.5 kg (0 ° C.) of the cryogen was stored in the

cryogen storage units

32 and 33, and the lid body 50 was put on the container body 10 again to close the opening of the container body 10.

Next, oxygen gas was supplied into the live fish transport container 1 from the lid through hole 51, which is a through hole near the tail side of the flounder F in the lid 50.

Next, the through-

holes

51 and 52 were closed with an adhesive tape to seal the inside of the live fish transport container 1 to obtain a package 100 for anhydrous transport. In FIG. 3, the adhesive tape does not depict the through hole formed by cutting out a part of the thin portion 11, the adhesive tape that closes the through hole, and the lid through

holes

51 and 52.

At this time, the temperature inside the live fish transport container 1 was 5 ° C., and the oxygen gas concentration was 80% by volume.

The temperature in the live fish transporting container 1 was measured using a digital temperature recorder “RTW30S” manufactured by ESPECMIC Co., Ltd. The oxygen gas concentration in the container was measured using an oxygen monitor “OXYMAN” manufactured by Taiho Engineering Co., Ltd.

[Evaluation of survival rate]
Thereafter, the package 100 containing the flounder in a low respiratory state (a state where the motion of the gills and the heart rate was reduced) was placed on a live fish transport truck (refrigerated car) and transported at a distance of 700 km over 12 hours. During transportation, the truck cabinet was opened and closed several times, so the temperature in the cabinet changed in the range of 3 to 20 ° C. However, the temperature inside the live fish container 1 during transportation was 7 ± 3 ° C. It was.

After such transport, the temperature inside the live fish transport container 1 was 6 ° C., and the oxygen gas concentration was 45% by volume.

Four flounder were taken out from the live fish transport container 1 and moved to a third water tank (size: 1 m long x 1 m wide x 0.5 m high) with a seawater temperature of 20 ° C. It was confirmed that the survival rate was 100%.

(Comparative Example 1)
<Induction of flounder to hypopnea>
A first water tank (size: length 1 m × width 1 m × height 0.5 m) maintained at a constant seawater temperature at the breeding temperature and a second water tank (size: length) capable of gradually cooling the seawater temperature 1 m × 1 m wide × 0.5 m height) was prepared.

The seawater temperature in the first tank was 10 ° C., and 4 flounder (about 1 kg / animal) were bred for 24 hours. Next, four flounder were moved to a second water tank having a seawater temperature of 10 ° C., and the seawater temperature was cooled to an acclimatization temperature of 4 ° C. over 16 hours at a cooling rate of 0.37 ° C./hr. Then, it acclimatized for 8 hours at the acclimatization temperature of 4 degreeC. In this way, 4 flounder were induced to a hypopnea state.

<Accommodating flounder in transport containers>
The live fish transport container 1 used in Example 1 was used as the live fish transport container. Four flounder F induced in a hypopnea state (a state where the motion of the gills and the heart rate decreased) were placed on the four trays 20 through the buffer sheet S, one by one.

Next, as shown in FIG. 3, the four trays 20 on which the flounder F was placed were arranged in two rows and two rows in the container body 10 with the tail placement portion 22 facing the same direction. In Comparative Example 1, seawater is not stored in the storage space of the container body 10. Spacing portions are formed between the tray 20 and the side wall 13 of the container body 10 and between the tray 20 and the bottom wall 12 of the container body 10, and in addition, a cooling agent is provided in the vicinity of the tail placement portion 22. The

accommodating parts

32 and 33 were formed.

Next, 0.5 kg (0 ° C.) of the cooling agent was stored in the cooling

agent storage portions

32 and 33, and the lid 50 was put on the container body 10.

Next, the inside of the container 1 for transporting live fish was sealed by closing the lid through

holes

51 and 52 of the lid 50 with an adhesive tape.

The temperature in the live fish transporting container 1 was measured using a digital temperature recorder “RTW30S” manufactured by ESPECMIC Co., Ltd. The oxygen gas concentration in the live fish transport container 1 was measured using an oxygen monitor “OXYMAN” manufactured by Taiho Engineering Co., Ltd.

[Evaluation of survival rate]
Then, the live fish transport container 1 containing the flounder F in a state of low breathing (a state where the motion of the gills and the heart rate decreased) was placed on a live fish transport truck (refrigerated car) and transported at a distance of 700 km over 12 hours. . During transportation, the truck cabinet was opened and closed several times, so the temperature in the cabinet changed in the range of 3 to 20 ° C. However, the temperature inside the live fish container 1 during transportation was 7 ± 3 ° C. It was.

4 flounder were taken out from the live fish transport container 1 and moved to a third water tank (size: 1 m in length x 1 m in width x 0.5 m in height), and 3 of the 4 flounder were revived. It was confirmed that the survival rate was 75%.

(Preliminary test)
The Japanese flounder (fishing temperature = about 10 ° C) caught in May near the sea near Japan and the Japanese flounder (fishing temperature = about 20 ° C) caught in September were put into the seawater in the tank. Was cooled by changing the temperature from plus 15 ° C. to around minus 2 ° C. at a rate of −1.0 ° C./hour, and the number of times of shark movement in seawater was measured.

運動 The number of movements of the cocoon was measured by installing a piezoelectric element on the cocoon of the flounder individual. The voltage generated according to the strain of the element was recorded and used as the number of movements of the heel. The average number of exercises per minute was determined every 20 minutes.

The results are shown in FIG. In the figure, “May” indicates a measured value of Japanese flounder caught in May, and “Sep” indicates a measured value of Japanese flounder caught in September.

As the seawater temperature decreases, the number of times the shark exercises decreases almost linearly, but in the case of an individual in May, the temperature range is -1 to + 2 ° C, and in the case of an individual in September, it is in a temperature range of +3 to + 5 ° C. In each case, the number of exercises of the heels became about 10 times / minute, and it became temporarily constant. When the temperature was further lowered, the number of exercises began to decrease again, eventually leading to death. The reason why the number of exercises stops decreasing in a specific temperature range is unknown, but metabolic compensation that keeps the metabolism at a constant level even when the temperature is low worked, so the number of exercises of the sputum simply decreases in the temperature range It is thought that it was kept constant.

On the other hand, it became clear that the minimum survival temperature depends on the seawater temperature at the time of fishing. From April to May, the seawater temperature was low and the low-temperature tolerance was expected to be high throughout the year. As a result of experiments with Japanese flounder caught in May, few individuals died at seawater temperature of 0 ° C, and some individuals survived to around -1.8 ° C when seawater began to freeze. On the other hand, since the seawater temperature was the highest in September, the low temperature tolerance was low and few individuals survived to near 0 ° C. From this result, it is proved that it is effective to set the temperature condition for inducing a fish individual to a low breathing state according to the water temperature at the time of fishing of the individual.

<Confirmation test of effect by resting breeding process>
(Example 2)
Four flounder (about 1 kg / animal) were bred for 24 hours in an aquarium filled with 18 ° C. seawater (aquarium size: length 1 m × width 1 m × height 0.5 m). This process was designated as “Livestock breeding process 1”. Livestock raising process 1 is also referred to as a cured animal raising process.

Next, the four flounder were moved to a container (container dimensions: width 448 mm × length 489 mm × height 140 mm) made of foamed plastics material with an internal volume of 30 L and containing 12 ° C. seawater, and a container with an aeration pump. The temperature was maintained at 12 ° C. for 16 hours while ventilating the inner seawater. This process was designated as “Livestock breeding process 2”. Livestock raising process 2 is also referred to as a resting livestock raising process.

After livestock raising step 2, the same live fish transport container 1 as that used in Example 1 was prepared, and four flounder F were placed on each of four trays 20 through buffer sheets S, one by one. Then, in the housing space of the container main body 10 filled with 12 ° C. seawater, the tail placement portions 22 were arranged in two rows and two rows in the same direction and submerged. At this time, surplus seawater overflowed out of the container and was discharged. Next, the opening of the container body 10 was closed with the lid 50. The lid 50 was closed so that the lid 50 did not come off the container body 10 due to the buoyancy of the tray 20.

While supplying air to the seawater in the live fish transport container 1 by the same method as in Example 1, it was left in a refrigerator set at −20 ° C. for 10 hours to cool the seawater temperature inside the box to 5 ° C. (this The average cooling rate in the cooling step was −0.7 ° C./hour). Assuming that 7 ° C. is the upper limit temperature of the second temperature range of the acclimation step, it can be said that the flounder fish body has been acclimated to the second temperature range of 7 ° C. or less for the last approximately three hours in this cooling step. After the 10-hour cooling step (after the acclimation step of about 3 hours), it was confirmed that flounder F was induced in a hypopnea state (a state in which the motion of the gills and the heart rate were reduced).

Next, the seawater in the live fish transporting container 1 is drained in the same procedure as in Example 1, the lid 50 is removed, the cold insulation agent (0 ° C.) is accommodated in the cold

insulation storage portions

32 and 33, and the lid 50 is again attached. The container main body 10 was covered and the opening of the container main body 10 was closed. After supplying oxygen gas according to the procedure described in Example 1, the inside of the live fish transport container 1 was sealed to obtain a package 100 for anhydrous transport. At this time, the temperature in the live fish transport container 1 was about 4 ° C., and the oxygen gas concentration was 80% by volume.

The live fish transport container 1 containing the flounder F obtained in the above procedure is placed on a live fish transport truck (refrigerated vehicle), and the temperature inside the live fish transport container 1 is kept at about 4 ° C. and transported over 25 hours. did.

After transportation, flounder was judged and the color, smell, and texture were confirmed. The confirmation results are shown in the following table, and a photograph of the half body including the spine is shown on the left of FIG.

(Example 3)
After performing the breeding process 1 of raising four flounder (about 1 kg / animal) in a water tank filled with seawater at 18 ° C. for 24 hours in the same manner as in Example 2, The same live fish transport container 1 as that used was prepared, and a cooling step and a transport step were performed. At this time, the seawater temperature in the live fish transport container 1 at the start of the cooling and acclimatization process is set to 18 ° C., and air is supplied to the seawater in the live fish transport container 1 in a refrigerator set to −20 ° C. It was left for 19 hours, and the seawater temperature inside the box was cooled to 5 ° C. (the average cooling rate in this cooling step was −0.7 ° C./hour). A cooling step and a transport step were performed. Assuming that 7 ° C. is the upper limit temperature of the second temperature range of the acclimation step, it can be said that the flounder fish body has been acclimated to the second temperature range of 7 ° C. or less for the last approximately three hours in this cooling step.

After transportation, flounder was judged and the color, smell, and texture were confirmed. The confirmation results are shown in the following table, and a photograph of the half body including the spine is shown on the right side of FIG.

<Confirmation test of effect by anhydrous transport>
Example 4
The same as that used in Example 1 without passing through the breeding process 2 after performing the breeding process 1 in which four flounder (about 1 kg / mouse) were raised for 24 hours in a water tank filled with seawater at 22 ° C. A container 1 for transporting live fish was prepared, and a cooling process and a transport process were performed. At this time, the seawater temperature in the live fish transporting container 1 at the start of the cooling process is set to 22 ° C., and air is supplied to the seawater in the live fish transporting container 1 while being kept in a refrigerator set at −20 ° C. for 24 hours. The cooling step was performed in the same procedure as in Example 2 except that the seawater temperature inside the box was cooled to 5 ° C (the average cooling rate in this cooling step was -0.7 ° C / hour). And carried out the transportation process. Assuming that 8 ° C is the lower limit temperature of the first temperature range of the animal breeding process and 7 ° C is the upper limit temperature of the second temperature range of the acclimatization process, the sole fish body is 7 ° C or less for the last approximately 3 hours in this cooling process. It can be said that it was adapted to the second temperature range. After the 24-hour cooling step (after the acclimation step of about 3 hours), it was confirmed that flounder F was induced in a hypopnea state (a state in which the motion of the gills and the heart rate were reduced).

insulation storage portions

32 and 33, and the lid 50 is again attached. The container main body 10 was covered and the opening of the container main body 10 was closed. After supplying oxygen gas according to the procedure described in Example 1, the inside of the live fish transport container 1 was sealed to obtain a package 100 for anhydrous transport. At this time, the temperature inside the live fish transport container 1 was about 7 ° C., and the oxygen gas concentration was 80% by volume.

The live fish transport container 1 containing the flounder F obtained in the above procedure is placed on a live fish transport truck (refrigerated car) and transported for 20 hours while maintaining the temperature inside the live fish transport container 1 at about 7 ° C. did.

After transportation, the flounder was handled and the color was confirmed. The white part was normal and the whiteness was normal. A photograph of the half body after the transportation, excluding the spine, is shown on the left of FIG.

(Comparative Example 2)
A container containing flounder is placed on a live fish transport truck (refrigerated car) while supplying air to the seawater without draining the seawater, and the seawater temperature in the container is kept at about 7 ° C and transported over 20 hours. Except for the above points, the livestock raising step and the cooling step were performed in the same procedure as in Example 4.

After transportation, the flounder was judged and the color was confirmed. As a result, many parts that were originally white were reddish due to bleeding. The photograph of the half body after the transportation excluding the spine is shown on the right side of FIG.

<Transport time confirmation test>
(Example 5)
A live fish transporting container 1 made of a foamed polystyrene-based resin molded body having a container body 10 and a lid 50 of the following size was prepared in the same form as shown in FIG. In order to accommodate the live fish F, a tray 60 having the same form as that shown in FIG. 14 was used, and the following buffer sheet S was installed on the bottom plate 63 thereof.

Container body 10: width 440 mm × length 575 mm × height 152 mm, side wall 13 thickness 20 mm, bottom wall 12 thickness 24 mm (container dimensions: width 404 mm × length 535 mm × height 128 mm, capacity: 28 L), expansion ratio 40 times (density 0.025 g / cm ³ ).

Lid 50: width 444 mm × length 575 mm × thickness 24 mm, expansion ratio 40 times (density 0.025 g / cm ³ ).

Tray 60: The thickness of the bottom plate 63 is 20 mm, the width 394 mm of the body placing portion 61 × 340 mm in length, the width 394 mm of the tail placing portion 62 × 190 mm in length, and the expansion ratio 40 times (density 0.025 g / cm ³ ).

Buffer sheet S: A polyurethane resin foam sheet is used. Thickness 5mm.

Two flounder (about 1 kg / animal) were bred for 24 hours in an aquarium filled with seawater at 14 ° C. (aquarium size: length 1 m × width 1 m × height 0.5 m). This process was designated as “Livestock breeding process 1”. Next, the two flounder were transferred to a container (container dimensions: width 300 mm × length 500 mm × height 200 mm) made of a foam plastic material having an internal volume of 30 L and containing 14 ° C. seawater. While ventilating the inner seawater, the mixture was gradually cooled to 12 ° C., and the temperature was maintained for 10 hours. This process was designated as “Livestock breeding process 2”.

After the livestock raising process 2, two flatfish F are placed on one tray 60 via the buffer sheet S in the prepared live fish transport container 1, and the other tray 60 is placed with the bottom plate 63 facing upward. Then, the flounder accommodation space was covered as much as possible and submerged in the accommodation space of the container body 10 filled with seawater at 12 ° C. At this time, surplus seawater overflowed out of the container and was discharged. Next, the opening of the container body 10 was closed with the lid 50. The lid 50 was closed so that the lid 50 did not come off the container body 10 due to the buoyancy of the tray 60.

While supplying air to the seawater in the live fish transport container 1, the container was left in a refrigerator set at −12 ° C. for 13 hours to cool the seawater temperature inside the box to 5 ° C. (the average cooling rate in this cooling step) Was −0.5 ° C./hour). Assuming that 7 ° C. is the upper limit temperature of the second temperature range of the acclimation step, it can be said that the flounder fish body has been acclimated to the second temperature range of 7 ° C. or less for the last approximately 4.5 hours in this cooling step. . After the cooling process for 13 hours (after the acclimation process of about 4.5 hours), it was confirmed that flounder F was induced in a hypopnea state (a state where the motion of the gills and the heart rate were reduced).

insulation storage portions

The live fish transport container 1 containing the flounder F obtained in the above procedure is placed on a live fish transport truck (refrigerated car) and transported over 30 hours while keeping the temperature inside the live fish transport container 1 at about 4 ° C. did.

After transportation, 2 flounder were taken out from the live fish container 1 and moved to a third water tank (size: 1 m long x 1 m wide x 0.5 m high). It was confirmed that the animals were revived (survival rate 100%). The flounder was judged, the color was not abnormal (white), the odor was not abnormal, the texture was moderately hard, and the overall evaluation was very good.

(Example 6)
Two flounder (about 1 kg / animal) were bred for 24 hours in an aquarium filled with 14 ° C. seawater (aquarium size: length 1 m × width 1 m × height 0.5 m). This process was designated as “Livestock breeding process 1”. Next, the two flounder were transferred to a container (container dimensions: width 300 mm × length 500 mm × height 200 mm) made of a foam plastic material having an internal volume of 30 L and containing 14 ° C. seawater. While ventilating the inner seawater, the mixture was gradually cooled to 12 ° C., and the temperature was maintained for 5 hours. This process was designated as “Livestock breeding process 2”.

After the livestock raising step 2, in the same manner as in Example 5, two flatfish F were placed on one tray 60 via the buffer sheet S in the prepared live fish transport container 1, and another tray 60 was covered with the bottom plate 63 facing upward so that the accommodation space for the flounder became as large as possible, and was immersed in the accommodation space of the container body 10 filled with seawater at 12 ° C. At this time, surplus seawater overflowed out of the container and was discharged. Next, the opening of the container body 10 was closed with the lid 50. The lid 50 was closed so that the lid 50 did not come off the container body 10 due to the buoyancy of the tray 60.

While supplying air to the seawater in the live fish transport container 1, the container was left in a refrigerator set at −12 ° C. for 10.5 hours to cool the seawater temperature inside the box to 5 ° C. (average in this cooling step) The cooling rate was -0.6 ° C / hour). Assuming that 7 ° C. is the upper limit temperature of the second temperature range of the acclimation step, it can be said that the flounder fish body has been acclimated to the second temperature range of 7 ° C. or less for the last approximately three hours in this cooling step. After the 10.5 hour cooling step (after the acclimation step of about 3 hours), it was confirmed that flounder F was induced in a hypopnea state (a state in which the motion of the gills and the heart rate were reduced).

Next, the seawater in the live fish transport container 1 is drained in the same procedure as in Example 5, the lid 50 is removed, the cold insulation agent (0 ° C.) is accommodated in the cold

insulation storage portions

The live fish transport container 1 containing the flounder F obtained in the above procedure is placed on a live fish transport truck (refrigerated car) and an airplane (room temperature, cargo), and the temperature inside the live fish transport container 1 is maintained at about 4 ° C. And transported over 12 hours.

1 ... Containers for transporting live fish (members for transporting live fish), 10 ... Container body, 12 bottom wall, 13 side walls, 14 ... ridges on side walls, 15 ... Spacers, 20 （trays Member), 21 body mounting portion, 22 tail mounting portion, 23 bottom plate, 24 ·· side plate, 25 ·· bottom plate corner through hole, 26 ·· bottom plate center through hole, 27 ·· stepped portion, 28 ·· depression , 31 .. Side wall separation part, 32 .. Cold insulation agent storage part, 33 .. Cold insulation agent storage part, 34 .. Bottom wall separation part, 35 .. Clearance, 50 .. Lid body, 51. , 52 .. Cover through-hole, F .. Fish (live fish), S ... Buffer sheet

All publications, patents and patent applications cited in this specification are incorporated herein by reference in their entirety.

Claims

A livestock raising process for raising fish in water at a temperature within the first temperature range;
An acclimatization step of acclimatizing fish in a live fish transport member containing water at a temperature in a second temperature range where the upper limit temperature is lower than the lower limit temperature of the first temperature range;
A draining step for draining water from the live fish transporting member;
An anhydrous transporting process for transporting fish while keeping them live in the live fish transporting member,
Including
The live fish transport member is a live fish transport container or a live fish transport tray,
Live fish anhydrous transport method.
The method for anhydrous transport of live fish according to claim 1, further comprising a cooling step of cooling the fish in water during or after the livestock raising process until the start or middle of the acclimatization process.
In the middle of or after completion of the livestock raising step, including a moving step of moving fish into a live fish transporting member containing water having a temperature T 1 within the first temperature range,
The cooling step is a step of cooling the temperature of water in the live fish transporting member to the temperature T 2 in the second temperature range after the moving step is completed.
The method for transporting anhydrous live fish according to claim 2.
The live fish transport member is a live fish transport container,
The moving step is
A step of placing the water temperature T 1 of in the live fish transport container,
Placing fish in a container for transporting live fish and immersing while discharging excess water;
The method for transporting anhydrous live fish according to claim 3, comprising:
After completion of the farming process, moving said first temperature range fish to live fish transport member in which accommodates the water at a higher temperature T 3 than the temperature T 2 of and the second temperature range lower than the lower limit temperature of the Including a moving process,
The cooling step is a step of cooling the temperature of the water in the live fish transporting member to T 2 after the end of the moving step.
The method for transporting anhydrous live fish according to claim 2.
The live fish transport member is a live fish transport container,
The moving step is
A step of placing the water temperature T 3 in the live fish transport container,
Placing fish in a container for transporting live fish and immersing while discharging excess water;
The method for transporting anhydrous live fish according to claim 5.
The cooling step is performed by changing the fish in water and the water temperature at a rate of -0.1 to -10 ° C / hour during or after the livestock raising step and before or during the acclimatization step. The method for transporting anhydrous live fish according to any one of claims 2 to 6, wherein the method comprises the steps of:
The cooling process cools the fish in water so that the absolute value of the change rate of the water temperature continuously decreases with time during the period from the middle or the end of the breeding process to the beginning or the middle of the acclimatization process. The method for anhydrous transport of live fish according to any one of claims 2 to 7, which is a process.
The method for anhydrous transport of live fish according to any one of claims 1 to 8, wherein an upper limit temperature and a lower limit temperature of the first temperature range are within a range of 8 to 30 ° C.
The breeding process includes breeding fish in water at a temperature equal to or lower than a threshold temperature P in the first temperature range;
The method for anhydrous transport of live fish according to any one of claims 1 to 9, wherein the threshold temperature P is a temperature within a range of 8 ° C or higher and 15 ° C or lower.
The farming process, the a farming step 1 of farmed fish in water of the first temperature region of the temperature T A, after the farmed step 1, the first temperature range, the temperature T is lower than the A temperature T B The method for anhydrous transport of live fish according to any one of claims 1 to 10, further comprising a livestock raising step 2 for raising fish in the water.
The method for anhydrous transport of live fish according to any one of claims 1 to 11, wherein an upper limit temperature and a lower limit temperature of the second temperature range are within a range of -2 to + 7 ° C.
The live fish according to any one of claims 1 to 12, further comprising a water temperature setting step of setting the first temperature range and / or the second temperature range in accordance with a temperature of water at the time of fish catch. Anhydrous transport method.
The live fish transport member is a live fish transport container,
The method for anhydrous transport of live fish according to any one of claims 1 to 13, wherein the container for transporting live fish is a heat-insulating plastics material.
The live fish anhydrous transport method according to claim 14, wherein the heat-insulating plastic material is foamed plastic.
The live fish transport member is a live fish transport container,
The anhydrous transport step comprises:
Performed by cooling the inside of the container for transporting live fish with a cooling agent,
The method for anhydrous transport of live fish according to any one of claims 1 to 15.
The anhydrous transport step comprises:
The oxygen concentration in the live fish transport member is set to 35% by volume or more.
The method for anhydrous transport of live fish according to any one of claims 1 to 16.
The method for anhydrous transport of live fish according to any one of claims 1 to 17, wherein the fish is a fish belonging to the family Floaceae.
In a live fish transport container that contains live fish in anhydrous water,
A container body having a bottom wall and a side wall erected from the periphery of the bottom wall; a lid for closing the opening of the container body; a tray having a bottom plate provided in the container body and on which live fish is placed; With
A spacing portion is formed between the tray and the side wall of the container body,
The bottom plate of the tray has a trunk placement portion and a tail placement portion,
A through hole is formed in the bottom plate of the tray,
A container for transporting live fish, wherein the container main body has a cryogen storage portion formed in the vicinity of the tail placement portion of the tray.
Furthermore, the container for transporting live fish according to claim 19, wherein a separation portion is formed between the tray and the bottom wall of the container body.
The bottom plate of the tray has a rectangular plate-like body placing portion that is rectangular in plan view, and a tail placement that extends from one of the short sides of the body placing portion and has a smaller width than the body placing portion. And
The live fish transport container according to claim 19 or 20, wherein the through hole is formed in the trunk mounting portion.
The tray has a side plate erected from the periphery of the bottom plate of the tray,
The live fish transport container according to any one of claims 19 to 21, wherein the side plate is partially lowered.
The live fish according to any one of claims 19 to 22, wherein a concave portion is formed on the bottom plate of the tray from the center of the trunk mounting portion toward the opposite direction to the tail mounting portion. Shipping container.
A plurality of the trays are provided;
The container for transporting live fish according to any one of claims 19 to 23, wherein the trays are overlapped with the tail mounting portion facing in the same direction.
A plurality of the trays are provided;
The container for transporting live fish according to any one of claims 19 to 24, wherein the trays are arranged with the tail mounting portions arranged in the same direction.
The container for transporting live fish according to any one of claims 19 to 25, wherein the container body is provided with a part capable of forming a through-hole or a drainage port that can be opened and closed.
A livestock raising process for raising fish in water at a temperature within the first temperature range;
Acclimating fish in water at a temperature in a second temperature range where the upper limit temperature is lower than the lower limit temperature of the first temperature range,
The farming process, the a farming step 1 of farmed fish in water of the first temperature region of the temperature T A, after the farmed step 1, the first temperature range, the temperature T is lower than the A temperature T B A method for inducing a hypopnea state in live fish including at least a livestock raising step 2 for raising fish in water.
The temperature at which the temperature T A exceeds a threshold temperature P,
Temperature T B is less temperature threshold temperature P,
28. The method according to claim 27, wherein the threshold temperature P is a temperature within a range of 8 ° C. or higher and 15 ° C. or lower.
The method according to claim 27 or 28, wherein the fish is a fish belonging to the family Floaceae.
After the completion of farmed step 1, the fish starts to farmed step 2 is moved in the water at a temperature T B, the method according to any one of claims 27-29.
The method according to any one of claims 27 to 30, wherein the acclimation step is performed while supplying air or oxygen into water.
The cooling step of cooling the fish while continuously reducing the water temperature in water during or after the livestock raising process until the start or middle of the acclimatization process. the method of.
The method according to any one of claims 27 to 32, wherein an upper limit temperature and a lower limit temperature of the first temperature range are within a range of 8 to 30 ° C.
The method according to any one of claims 27 to 33, wherein an upper limit temperature and a lower limit temperature of the second temperature range are within a range of -2 to + 7 ° C.
The method according to any one of claims 27 to 34, further comprising a water temperature setting step of setting the first temperature range and / or the second temperature range in accordance with a temperature of water at the time of fish catch. .