WO2017030455A1 - Puce microfluidique comprenant un système de régulation de pression et de débit - Google Patents

Puce microfluidique comprenant un système de régulation de pression et de débit Download PDF

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Publication number
WO2017030455A1
WO2017030455A1 PCT/PL2016/000087 PL2016000087W WO2017030455A1 WO 2017030455 A1 WO2017030455 A1 WO 2017030455A1 PL 2016000087 W PL2016000087 W PL 2016000087W WO 2017030455 A1 WO2017030455 A1 WO 2017030455A1
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WO
WIPO (PCT)
Prior art keywords
piston
fabricated
plate
lead screw
channel
Prior art date
Application number
PCT/PL2016/000087
Other languages
English (en)
Inventor
Krzysztof MELLEM
Paweł ZAWADZKI
Kamil Robert GEWARTOWSKI
Original Assignee
Curiosity Diagnostics Sp. Z O.O.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Curiosity Diagnostics Sp. Z O.O. filed Critical Curiosity Diagnostics Sp. Z O.O.
Publication of WO2017030455A1 publication Critical patent/WO2017030455A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883—Serpentine channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons

Definitions

  • the invention provides a microfluidic chip with pressure and flow control system that may be used for rapid molecular tests, in particular in micro-analytical testing, clinical diagnostics, forensics, biochemistry and molecular biology, allowing for detection and identification of pathogens, analysis of genetic material, blood diagnostics, and also detection and identification of various chemicals and/or biomaterials in samples.
  • microfluidic systems with chips fabricated from polymer or glass plates with a size comparable to credit card or less.
  • Microfluidic systems for molecular tests are usually combined with optical or optoelectronic devices that allow for testing and carrying out measurements, and also with devices used to control the analytical process in the chip and to process and record data.
  • Inside the chip there is a system of microfluidic channels guiding and mixing liquids, with junctions connected to separation channels, measurement cells and an outlet channel.
  • the flow of fluids in microchannels is controlled using a pressure method by means of a pressure and flow control system attached to the chip.
  • Such a system includes fluid reservoirs, micropumps, pressure regulators and valves. External devices for precise fluid dispensing, such as syringe pumps, are also used.
  • microfluidic chips integrated with a membrane microvalve aligned perpendicularly to the plate comprising the microfluidic system, which controls pumping of a liquid and/or gas in microchannels. It requires, however, that a microvalve is connected to a precise actuator with a driver controlling the opening or closing time of the valve, while simultaneously controlling the movement of the fluid.
  • a pneumatic microvalve designed for generation of liquid droplets and gas bubbles in dispersed liquid is disclosed in the PL 213900 patent description.
  • Such a microvalve is not suitable for forcing a laminar flow of a liquid that makes up the continuous phase.
  • the microfluidic chip reported in the above paper comprises pistons located in chambers with reagents, over the channels connecting the measurement cells. After insertion of the chip into a device used to carry out the reaction, the pistons are activated with electronically controlled pneumatic valves, which results in pumping of the reagents to channels. In addition, the pistons make the microfluidic system tight and separated from the environment within the chip, thus minimising the risk of reagent contamination.
  • the purpose of the present invention is to integrate the pressure and flow control system with a microfluidic chip designed for performing single tests, allowing for running the analytical process under pressurised conditions while minimising the requirements related to specialised laboratory equipment.
  • the invention provides a microfluidic chip comprising a pressure and flow control system, making up a plate together with a microfluidic system connected inside the plate with at least two chambers filled with fluids used in the analytical process, each of the said chambers comprising an inlet channel fabricated in the plate, with a piston placed within the said channel and sealing the microfluidic system, characterised in that each inlet channel is furnished with an internal self-locking thread, fabricated from the side of the inlet opening, in which said channel a lead screw connected to the piston is set.
  • the thread of the lead screw fits the internal thread with no clearance.
  • the lead screw is a bolt screw with a screw drive formed on the external end.
  • the lead screw is furnished with a measurement indicator at the external end.
  • the piston is a plunger in the form of a cylindrical end of the lead screw set slidingly in a gland fixed in the inlet channel.
  • the inlet channel is terminated on the side of the inlet opening with a holder fixed to the plate, and the internal thread, in which the lead screw is set, is fabricated in the holder.
  • the holder is separably fixed to the plate.
  • the inlet channel and the axis of rotation of the lead screw with the piston of either chamber are situated in parallel to the surface of the plate.
  • the inlet channel comprises, in the wall of the cylindrical section, where the piston is moving, a side inlet minichannel fabricated in the plate.
  • the piston is a disc piston, whereas the height of the said piston at the contact with the wall of the cylindrical section of the inlet channel, where the piston is moving, is greater than the diameter of the microchannel fabricated in the said wall, connecting the chamber with the microfluidic system.
  • the piston is a final section of the lead screw with the same thread pitch fitting with no clearance the internal thread of the inlet channel, fixed to the lead screw from the chamber side.
  • the microfluidic chip with a pressure and flow control system making up a plate together with a microfluidic system connected inside the plate with at least two chambers filled with fluids used in the analytical process, each of the said chambers comprising an inlet channel fabricated in the plate together with a piston placed within the channel and sealing the microfluidic system, is characterised in that each inlet channel is furnished with an internal self-locking thread fabricated from the side of the inlet opening, in which said channel a lead screw connected to the piston is set, whereas the microfluidic system fabricated in the plate is composed of a network of channels filled with fluids, connecting the chambers with an analytical cavity, and the analytical cavity with the measurement zone and the separation zone, wherein a reservoir is fabricated.
  • the first chip variant is distinguished by that the analytical cavity is connected to the measurement zone and the separation zone through a junction with two minichannels furnished with closing valves, whereas one rmnichannel is connected with a reservoir, and the second with a system of measurement cells fabricated in the measurement zone.
  • the second chip variant is distinguished by that a measurement channel is fabricated in the measurement zone and connects the analytical cavity with the separation zone through a junction with two minichannels furnished with closing valves, whereas one minichannel is connected to a reservoir, and the second to a drain well, and a third chamber furnished with a screw mechanism comprising a lead screw connected to the piston is attached to the channel network connecting the chambers with the analytical cavity.
  • the chip according to the present invention is particularly suitable for use in reactions requiring generation of a high overpressure, i.e., in the range 1-10000 bar, which require application of a large force, in the range 10-100000 N per piston. Because of the use of a screw connection, these forces are distributed over the thread surface. Due to the fact, it is not necessary to apply a so large force per piston, and consequently it allows for simplification of the design of an adapter for the plate, as opposed to microfluidic systems with an external control system. The use of a self-locking screw allows to maintain a high pressure in the channel without continuous application of the torque.
  • Such a chip assures that the tightness and a stationary position of the piston are maintained after filling of the microfluidic system, and during its storage and transport.
  • the use of the screw because of its mechanical advantage, allows for dispensing fluids with high precision. For example, for a screw pitch of 1 mm and resolution of rotation of about 5 degrees, the accuracy of the piston positioning is about 14 ⁇ . Because of that, with a scale and an indicator on the screw, reactions in a microfluidic system using the present invention may be carried out manually.
  • the screw mechanism allows also for generation of underpressure in the chambers, which may be used for pre-fdling or refilling of fluids.
  • Fig. la shows a microfluidic chip used for the PCR reaction, in a longitudinal section in the plane parallel to the plate
  • Fig. lb shows a microfluidic chip used in the HPLC analysis, in a longitudinal section in the plane parallel to the plate
  • Fig. 2 shows a section of the chip comprising the screw mechanism positioned perpendicularly to the plate, in a cross section
  • Fig. 3 shows a section of the chip comprising the screw mechanism positioned
  • Fig. 4 shows a section of the chip fabricated from a uniform material, comprising the screw mechanism positioned parallel to the plate, in a cross section
  • Fig. 5a shows a section of the chip comprising the screw mechanism positioned perpendicularly to the plate, with two microchannels connected to a chamber, in partial cross section
  • Fig. 5 b shows a section of the chip presented in Fig. 5 a, with microchannels closed by the piston, in partial cross section
  • Fig. 6 shows a section of the chip comprising the screw mechanism positioned perpendicularly to the plate, with external holder on the extension of the inlet channel and the holder fixation, in a cross section
  • FIG. 7 shows a section of the chip comprising the screw mechanism positioned perpendicularly to the plate, with plunger, in a cross section
  • Fig. 8 shows a section of the chip comprising the screw mechanism positioned parallel to the plate, with a side inlet minichannel fabricated in the plate, in a cross section
  • Fig. 9 shows a cross section of a section of the chip comprising the screw mechanism positioned parallel to the plate, where the piston makes up the terminal section of the lead screw with the same thread pitch.
  • the microfluidic chip with a pressure and flow control system makes up a plate 10 with microfluidic system connected inside the plate 10 with two chambers 1 , 2, filled with reagents used in the analytical process.
  • Each chamber comprises an inlet channel 3 fabricated in the plate 10 with a piston 5 placed within the channel and sealing the microfluidic system.
  • Each inlet channel 3 is furnished with an internal self- locking thread, fabricated from the side of the inlet opening, in which said channel the lead screw 4 connected to the piston 5 is set.
  • the lead screw 4 set in the internal thread makes up a screw mechanism, shown in Fig. 2-8, allowing to position the piston 5 in the inlet channel 3.
  • the lead screw is a bolt screw with a screw drive formed on its external end.
  • the thread of the lead screw 4 fits the internal thread with no clearance. Fitting with no clearance means a tight fitting, wherein the clearance has a negative value, or a sliding fitting, wherein the clearance assumes a zero value. Such a fitting increases the precision of piston 5 positioning, and functions as a sealing.
  • the lead screw 4 comprises a measurement indicator at the external end. When a fixed scale is used to read out the change in the angular position of the indicator following rotation of the screw around the axis, a precise determination of the position of the piston 5 is possible. At the same time, the screw mechanism inducing the movement of the piston 5 allows for generation of an appropriate pressure in the microfiuidic system fabricated in the plate 10.
  • Coordinated operation of screw mechanisms allows for pressure regulation and controlling the flow within the microfiuidic system and its junctions inside the plate 10.
  • the inlet channel 3 and the axis of rotation of the lead screw 4 with the piston 5 of either chamber 1, 2 may be oriented perpendicularly or parallel to the surface of the plate 10. It does not exclude a design, wherein the channel and the screw mechanism occupy an intermediate position.
  • the microfiuidic system of the chip presented in Fig. la comprises a densely packed channel network filled with fluids, which may be separated from each other by a layer of a hydrophobic fluid.
  • Chambers 1 , 2 may be filled with a hydrophobic fluid, for example a mineral oil, which mediates pumping of the reagents.
  • the channel network connects the outlet minichannels.
  • Systems are also used wherein each fluid is comprised in different chamber 1, 2, whereas the outlet channels of the said chambers join before the analytical cavity 11, which may be filled with an agent interacting with the tested sample, for example with a silica bed or suitable reagents.
  • the analytical cavity 11 is connected to the measurement zone and the separation zone via a junction comprising two minichannels fiirnished with the closing valves 15.
  • the channels deliver the pumped fluid to the analytical cavity 11, and then to the measurement zone comprising a system of distributed radially measurement cells 16 or to a separating minichannel connected to a tight waste reservoir 12.
  • the measurement cells 16 may be also distributed in multiple rows or otherwise.
  • the closing valve 15 may be fabricated in the form of a screw mechanism as presented in Fig. 5 a, 5b, or as a conventional membrane valve.
  • the closing valves 15 allow for separation of pumped fluids, for example the washing liquids guided to the reservoir from the eluate pumped to the measurement cells 16.
  • the microfiuidic system of the chip presented in Fig. lb comprises also chambers 1, 2 connected to a densely packed channel network, and a third chamber, whereas the outlet channel of the third chamber is connected to the outlet channel of the second chamber 2. All the chambers are furnished with a screw mechanism with the lead screw 4 connected to the piston 5. Each of these chambers may be filled with different fluid used in the analytical process.
  • the channels deliver the pumped fluid to the analytical cavity 11, and then to the measurement zone comprising the measurement channel 13.
  • the measurement channel 13 is connected to the separation zone via a junction with two minichannels. Both minichannels are furnished with the closing valves 15.
  • the first minichannel is connected with the waste reservoir 12, and the second with the drain well 14.
  • the screw mechanism may be accomplished in accordance with the essence of the invention in different embodiments shown in Fig. 2-8.
  • the internal thread is fabricated in the upper layer of the plate 10, and the lead screw 4 is set perpendicularly to the surface of the plate 10.
  • the screw mechanism allows for an easy delivery of the sample or the reagent to the microfluidic system by loosening the lead screw 4 and puling it out together with the piston 5 from the inlet channel 3 to the extent enabling the transferring of the fluid to the chamber 1, 2 with known methods, for example by pippeting. Following sample delivery, the system can be sealed again, and the screw drive can be used to control further flow of the fluid.
  • the internal thread used to set the lead screw 4 is fabricated in the holder 6 fixed separably to the plate 10 at the end of the inlet channel 3 positioned parallel to the surface of the plate 10.
  • the holder 6 may be separated together with the lead screw 4 and the piston 5 from the chip, thus allowing for faster delivery of the sample or the reagent to chamber 1,2.
  • the system can be sealed again, and the screw drive can be used to control further flow of the fluid.
  • the internal thread is fabricated in the side of the plate 10, and the diameter of the area, where the piston 5 is moving, is less than that of the threaded section.
  • the system can be sealed again, and the screw drive can be used to control further flow of the fluid.
  • the internal thread used to set the lead screw 4 is fabricated in the holder 6 fixed separably to the plate 10, and the inlet channel 3 is positioned perpendicularly to the surface of the plate 10.
  • the holder 6 may be separated together with the lead screw 4 and the piston 5 from the chip.
  • the inlet channel 3 comprises in the cylindrical section, where the piston 5 is moving, a microchannel connecting the chamber 1, 2, filled with a fluid used in the analytical process, with the microfluidic system.
  • the piston 5 is a disc piston, whereas the height of the said piston at the contact with the wall of the cylindrical section of the inlet channel 3, where the piston is moving, is greater than the diameter of the microchannel 8 fabricated in that wall, connecting the chamber 1, 2 with the microfluidic system. As shown in Fig. 5b, it allows for closing the fluid flow in this area of the plate 10.
  • a microvaive may be placed in front of the zone comprising the measurement cells and the zone with the buffers and the waste reservoir.
  • the microchannel 8 may be fabricated at the base of the chamber or in another zone of the inlet channel 3, where the piston 5 is moving.
  • piston microvalves can replace conventional valves closing the flow to selected areas of the microfluidic system.
  • the internal thread used to set the lead screw 4 is fabricated in the holder 6 fixed separably to the plate 10 at the end of the inlet channel 3 positioned perpendicularly to the surface of the plate 10.
  • the lower section of the holder is set in an anchorage fabricated in the plate 10, and is protected by the plug 9, set in the channel fabricated in the side of the plate 10. From the side, the holder 6 may be separated together with the lead screw 4 and the piston 5 from the chip.
  • the sample or a reagent can be delivered after the holder 6 is separated and the screw removed together with the piston 5.
  • the piston is a plunger in the form of a cylindrical end of the lead screw 4 set slidingly in the gland 5' fixed in the inlet channel 3.
  • a minichannel connecting the space over and under the piston can be fabricated in the screw. It can be used for delivering the sample or a reagent and/or for installation of a sensor measuring the process parameters.
  • the plunger provides a more effective sealing than a disc piston.
  • the inlet channel 3 comprises, in the cylindrical section, where the piston 5 is moving, a side inlet minichannel 7 fabricated in the plate 10. It allows for delivering the sample or a reagent to the chamber 1, 2 without removing the piston 5, after the said piston is displaced to the upper position with the screw mechanism, above the edge of the side inlet minichannel 7.
  • the process can be carried out using the underpressure generated by the piston.
  • the minichannel may be also used for venting a filled microfluidic system. After displacing the piston to the lower position, the minichannel is cut off from the content of the chamber 1, 2 and does not affect the regular operation of the system.
  • the inlet channel 3 is threaded along its entire length, and the .piston 5 is a final section of the lead screw 4 of the same thread pitch, fitting with no clearance the internal thread of the inlet channel 3, fixed to the lead screw 4 from the side of the chamber 1, 2.
  • the piston is moved together with the screw by a rotating-sliding motion.
  • the disclosed embodiment variants of the screw mechanism allow for an easy filling of the chip with the sample or a reagent so as to allow for immediate starting of the reaction.
  • the chambers 1, 2 may also be filled with a hydrophobic fluid, for example a mineral oil, which mediates pumping of the reagents.
  • a hydrophobic fluid for example a mineral oil, which mediates pumping of the reagents.
  • the fluids involved in the reaction may be separated from each other by a layer of a hydrophobic fluid.
  • Systems are also in use where each of the fluids is contained in different chamber 1, 2, whereas the outlet channels of the chambers are joined in front of the bed. An additional valve may be placed at the outlet of each chamber 1, 2.
  • the screw mechanism with the piston 5 is an integral part of a single use chip, the invention allows for elimination of problems with contamination of external pumps, which, unlike the chip, are used repeatedly.
  • the lead screw may be furnished with a typical head or a terminal allowing for its rotation.
  • the use of the lead screw 4 with appropriate screw drive on its external end enables fabrication of microfluidic systems which are resistant to accidental or intentional tampering. In such a case, a special key would have to be used to enable a displacement of fluids comprised inside the chip.
  • One of the typical applications of the chips disclosed herein is to use them in PCR (polymerase chain reaction-based) reactions carried out under pressurised conditions and used for fast detection of pathogens. Because of the sensitivity of the method, which is able to detect even a single DNA fragment, a major problem is the possibility of contaminating the chip or a device where the chip is located. Such contamination may lead to erroneous results. Therefore it is desired to use a chip that is entirely closed or isolated from its surrounding to the greatest possible extent.
  • the use of a high pressure source in the microfluidic chip allows for elirnination of drains that are usually required for filling of the measurement cells, which significantly increases the system resistance to contamination.
  • Application of the present invention allows for filling the measurement cells by
  • the compression ratio depends on the ratio of the initial to final pressure, and therefore precise fillings at any level can be achieved.
  • the filling of the measurement cells is uniform. It provides also a dependable method for verifying if all cells are correctly filled, consisting in a measurement of the final pressure in the system.
  • the pressure can be measured with optical method by measuring the diameter of air bubbles in the measurement cells and comparing the initial and final volumes of the air filling the system.
  • One can also measure the force acting on the piston by measuring the torque exerted by the drive that screws in the screw.
  • the chips used to carry out the PCR reactions comprise the lower layer made of a 0.1 mm thick foil, and after filling under high pressure the foil under the measurement cells becomes bumpy. It is of major importance for PCR, as the cells are in continuous thermal contact with a heating unit, to which they are pressed.
  • the correct temperature control of the sample requires that the thermal conductivity, and in particular the thermal contact conductance, is precisely controlled.
  • a deformation of the foil over the cells levels the microunevenness between the surface of the heating unit and the chip, which significantly increases the thermal conductivity and the reaction reproducibility, it should be stressed that carrying out the reaction under pressurised conditions eliminates the problems with air bubbles, which are usually formed in the course of PCR.
  • the bubbles are formed as a result of the change of gas solubility in liquid and they increase their size with increasing temperature, which may lead to a change of temperature conditions of the reaction, or even to squeezing out the liquid from the measurement cell.
  • the use of high pressure does not allow for formation and expansion of air bubbles, which significantly increases the reliability of the entire system.
  • Another application of the disclosed chips is to use them for DNA isolation.
  • one of the screw mechanisms is used for delivering the solution comprising the sample taken from the patient and pushing it through a DNA binding bed.
  • the second screw mechanism is used for pushing through the bed the washing solutions and elution solutions, which were placed before reaction in appropriate order in a snakelike channel and separated from each other by layers of a hydrophobic fluid.
  • Valve-controlled junctions allowing for separation of wastes generated by the isolation process from the elution may be provided at the outlet of the channel comprising a bed capable of DNA binding.
  • the use of the screw mechanism allows for generation of a high pressure that is required for pushing the fluids through the bed that may be densely packed. It provides also an excellent control over the isolation process, and in particular allows to determine the moment of flow of the target eluate to be separated from the remaining buffers used in the process.
  • the system for isolation and the system for PCR are connected so that the eluate, after leaving the system for isolation, under pressurised conditions fills the wells on the PCR chip, which may comprise lyophilised reagents required to carry out the reaction.
  • the solution according to the present invention can also be used in HPLC.
  • one of the screw mechanisms is used to deliver the tested sample and to guide it to the bed.
  • Another screw mechanism is used to push through the bed the carrier liquid, which initially filled its chamber.
  • the analysis can be performed using the gradient method.
  • the components of the analysed sample could be detected in the measurement channel 13 using a known method, for example by measuring absorbance in UV.
  • the use of the closing valves 15 allows to guide selected components to the drain well 14, where they can be collected and delivered for further analysis.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

L'invention concerne une puce microfluidique comprenant un système de régulation de pression et de débit constituant une plaque conjointement avec un système microfluidique raccordé à l'intérieur de la plaque (10) à au moins deux chambres (1, 2) remplies de fluides utilisés dans le processus analytique, chacune desdites chambres comprenant un canal d'entrée (3) fabriqué dans la plaque (10), un piston (5) étant placé à l'intérieur dudit canal et assurant l'étanchéité du système microfluidique. Chaque canal d'entrée (3) est doté d'un filetage autobloquant interne fabriqué à partir du côté de l'ouverture d'entrée, une vis-mère (4) raccordée au piston (5) étant définie dans ledit canal. Le système microfluidique fabriqué dans la plaque (10) comprend de préférence un réseau de canaux remplis de fluides raccordant les chambres (1, 2) à une cavité analytique (11), la cavité analytique (11) comprenant la zone de mesure et la zone de séparation, un réservoir (12) étant fabriqué.
PCT/PL2016/000087 2015-08-18 2016-08-14 Puce microfluidique comprenant un système de régulation de pression et de débit WO2017030455A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PLP.413589 2015-08-18
PL413589A PL413589A1 (pl) 2015-08-18 2015-08-18 Chip mikrofluidyczny z układem sterowania ciśnieniem i przepływem

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107542973A (zh) * 2017-08-02 2018-01-05 南京岚煜生物科技有限公司 具有闭锁阀门的微流控芯片及其使用方法
CN108686724A (zh) * 2017-04-10 2018-10-23 苏州含光微纳科技有限公司 一种微流控时控阀门
RU2703776C1 (ru) * 2019-01-25 2019-10-22 Российская Федерация в лице Министерста здравоохранения Одноразовый чип для проведения пцр анализа
CN112808333A (zh) * 2020-12-29 2021-05-18 厦门大学 微流控芯片夹具
CN113275044A (zh) * 2020-02-20 2021-08-20 北京京东方健康科技有限公司 检测芯片及其使用方法、检测装置
WO2024012370A1 (fr) * 2022-07-13 2024-01-18 清华大学 Capteur de pression microfluidique, procédé et dispositif de mesure de pression, et support de stockage associé

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL213900A1 (fr) 1979-03-05 1980-12-15 Os Bad Rozwojowy Urzadzen Info
US6645758B1 (en) * 1989-02-03 2003-11-11 Johnson & Johnson Clinical Diagnostics, Inc. Containment cuvette for PCR and method of use
WO2014149277A2 (fr) * 2013-03-16 2014-09-25 Roberts Leslie Don Cartouche d'analyse modulaire et autonome et système programmable de distribution de réactif
US20150099291A1 (en) * 2005-05-09 2015-04-09 Biofire Diagnostics, Llc Self-contained biological analysis

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL213900A1 (fr) 1979-03-05 1980-12-15 Os Bad Rozwojowy Urzadzen Info
US6645758B1 (en) * 1989-02-03 2003-11-11 Johnson & Johnson Clinical Diagnostics, Inc. Containment cuvette for PCR and method of use
US20150099291A1 (en) * 2005-05-09 2015-04-09 Biofire Diagnostics, Llc Self-contained biological analysis
WO2014149277A2 (fr) * 2013-03-16 2014-09-25 Roberts Leslie Don Cartouche d'analyse modulaire et autonome et système programmable de distribution de réactif

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CN108686724A (zh) * 2017-04-10 2018-10-23 苏州含光微纳科技有限公司 一种微流控时控阀门
CN107542973A (zh) * 2017-08-02 2018-01-05 南京岚煜生物科技有限公司 具有闭锁阀门的微流控芯片及其使用方法
CN107542973B (zh) * 2017-08-02 2023-11-28 南京岚煜生物科技有限公司 具有闭锁阀门的微流控芯片及其使用方法
RU2703776C1 (ru) * 2019-01-25 2019-10-22 Российская Федерация в лице Министерста здравоохранения Одноразовый чип для проведения пцр анализа
RU2703776C9 (ru) * 2019-01-25 2020-02-18 Российская Федерация в лице Министерства здравоохранения Одноразовый чип для проведения пцр анализа
CN113275044A (zh) * 2020-02-20 2021-08-20 北京京东方健康科技有限公司 检测芯片及其使用方法、检测装置
CN113275044B (zh) * 2020-02-20 2022-07-12 北京京东方健康科技有限公司 检测芯片及其使用方法、检测装置
CN112808333A (zh) * 2020-12-29 2021-05-18 厦门大学 微流控芯片夹具
WO2024012370A1 (fr) * 2022-07-13 2024-01-18 清华大学 Capteur de pression microfluidique, procédé et dispositif de mesure de pression, et support de stockage associé

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