WO2017026429A1 - Muscle synthesis promoting agent - Google Patents
Muscle synthesis promoting agent Download PDFInfo
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- WO2017026429A1 WO2017026429A1 PCT/JP2016/073233 JP2016073233W WO2017026429A1 WO 2017026429 A1 WO2017026429 A1 WO 2017026429A1 JP 2016073233 W JP2016073233 W JP 2016073233W WO 2017026429 A1 WO2017026429 A1 WO 2017026429A1
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- protein
- whey protein
- leu
- muscle synthesis
- muscle
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Definitions
- the present invention relates to a muscle synthesis promoter.
- Whey protein contains many BCAAs (branched chain amino acids: valine, leucine, isoleucine), and has a fast rate (absorption rate) from oral intake to digestion absorption (because of the short time), so it has fast-acting muscle synthesis. It is known that muscles can be efficiently strengthened while preventing muscle fatigue by showing a promoting effect and ingesting especially during exercise. On the other hand, the casein protein is not suitable for subjects who need an immediate effect of promoting muscle synthesis because the rate from absorption to digestion (absorption rate) is slower (longer time) than whey protein. It is said that there is (see Patent Document 1). Therefore, whey protein is mainly used as protein powder.
- BCAAs branched chain amino acids: valine, leucine, isoleucine
- whey protein can be made from whey that is discharged after curding raw milk with acid, rennet, and heating in the natural cheese production process. Since it can be reused without being discarded, it is significant to use whey protein in terms of environment.
- whey protein has an immediate effect of promoting muscle synthesis, but it naturally requires ingestion of a predetermined amount of whey protein in order to achieve this effect. Ingestion of such a predetermined amount of whey protein sometimes makes it difficult for infants and the elderly who cannot take a large amount of energy, but even for healthy adults, the intake energy and protein intake There are also concerns about the onset of lifestyle-related diseases such as obesity and the induction of dysfunction of the liver, kidneys, etc., due to an excess of the above. In addition, taking whey protein itself may be a psychological burden regardless of age.
- whey protein has a sufficiently immediate effect of promoting muscle synthesis, and it is surmised that the speed of whey protein absorbability contributes to this.
- the absorption of whey protein into the body is extremely fast compared to other proteins, and so far, it is thought that there is no one that has the same effect of promoting muscle synthesis with less intake (dose) than whey protein. It was done. Therefore, although it is true that there is a situation where the intake of the whey protein is a burden, it is not always a large amount of protein that has the same or higher muscle synthesis promoting effect with a lower intake (administration) amount than the whey protein.
- a muscle synthesis promoter that does not require ingestion has not been known so far. Therefore, it has been a problem to provide a new muscle synthesis promoter that exhibits such effects.
- the present inventors have found a muscle synthesis promoter comprising a whey protein hydrolyzate as an active ingredient, and further, the muscle synthesis promoter is converted into whey protein.
- the present inventors have found that it can be used as a protein source that exhibits the same or higher muscle synthesis promoting effect at a lower intake (administration) amount than whey protein, and does not necessarily need to take a large amount of protein.
- the present inventors have rapidly absorbed the whey protein itself into the body, there is no one that exhibits the same level of muscle synthesis promoting effect with less intake (administration) than the whey protein.
- the present inventors have unexpectedly found that the whey protein hydrolyzate has a more immediate effect of promoting muscle synthesis compared to the whey protein itself.
- the whey protein hydrolyzate of the present invention was comparable to whey protein and was expected to exhibit the same muscle synthesis promoting effect as whey protein. It was unexpectedly found that the whey protein hydrolyzate of the invention has a further immediate effect of promoting muscle synthesis as compared with conventional whey proteins. The above phenomenon was surprising. The present invention is based on these findings.
- a muscle synthesis promoter comprising whey protein hydrolyzate as an active ingredient
- the whey protein hydrolyzate contains a dipeptide, and the dipeptide (1) Ile-Leu and Leu-Ala, and (2) Ala-Leu, Val-Leu, Lys-Ile, Leu-Leu, Ile-Asn, Leu-Glu, Leu-Val, and Ile-Val
- the muscle synthesis promoter according to [1] which is at least one dipeptide selected from the group
- the proteolytic enzyme is one or more selected from Bacillus genus proteolytic enzyme, Aspergillus genus proteolytic enzyme, trypsin, pepsin, and flavorzyme
- a method for producing a muscle synthesis promoter comprising blending an effective amount of a whey protein hydrolyzate.
- a method for promoting muscle synthesis which comprises allowing a subject in need thereof to take an effective amount of a whey protein hydrolyzate, [13]
- the whey protein hydrolyzate contains a dipeptide, and the dipeptide (1) Ile-Leu and Leu-Ala, and (2) Ala-Leu, Val-Leu, Lys-Ile, Leu-Leu, Ile-Asn, Leu-Glu, Leu-Val, and Ile-Val
- the method for promoting muscle synthesis according to [12] which is at least one dipeptide selected from the group, [14]
- a muscle synthesis promoter containing whey protein hydrolyzate as an active ingredient can be provided.
- the muscle synthesis promoter of the present invention can be used as a protein source in place of whey protein, which has the same or higher muscle synthesis promoting effect with less intake (administration) amount than whey protein, and does not necessarily need to consume a large amount of protein. can do. Since the muscle synthesis promoter of the present invention uses whey protein hydrolyzate as an active ingredient, the burden of digestion and degradation on the subject is lighter than that of whey protein.
- the muscle synthesis promoter of the present invention is particularly advantageous in that it can exert an excellent muscle synthesis promoting action while reducing the digestive burden on the subject, in addition to exhibiting an effect with a less intake amount than whey protein. It is. Moreover, whey protein is usually discharged after curding raw material milk with acid, rennet, heating, and the like in the production process of natural cheese, and discarded to become industrial waste. The whey protein hydrolyzate contained as an active ingredient in the muscle synthesis promoter of the present invention can be used as a raw material by reusing the whey that is industrial waste. The significance of the invention is great.
- the muscle synthesis promoter of the present invention is a muscle synthesis promoter containing whey protein hydrolyzate as an active ingredient.
- Active ingredient means an ingredient required for exerting a muscle synthesis promoting effect.
- the “active ingredient” means a component that is required to exert a muscle synthesis promoting effect for a subject that needs immediate muscle synthesis promotion.
- “Promoting muscle synthesis” means promoting the synthesis of muscle by ingesting whey protein hydrolyzate and absorbing it in the body as a nutrient. Therefore, the muscle synthesis promoter of the present invention is used for that and the state resulting therefrom.
- the purpose of promoting muscle synthesis includes muscle gain, muscle growth promotion, muscle tissue repair, muscle recovery, muscle enhancement, muscle strength improvement, health promotion effect and muscle performance improvement associated with muscle synthesis promotion. Health improvement effect and exercise ability improvement accompanying tissue repair, muscle strength improvement, muscle synthesis promotion. Promoting muscle synthesis also includes assimilation of muscle proteins from amino acids.
- “Immediately effective muscle synthesis promotion” means that muscle synthesis is promoted by the immediate absorption of whey protein hydrolyzate.
- the muscle synthesis promotion of the present invention includes muscle synthesis promotion in a healthy person.
- Subjects requiring immediate muscle synthesis promotion means subjects who need to promote muscle protein synthesis in order to restore muscle tissue and promote muscle growth.
- it means a subject to exercise in order to promote muscle synthesis, prevent muscle loss, and promote muscle mass recovery.
- the exercise is preferably strength training (resistance exercise), and more preferably long-term strength training, for example, in that muscle synthesis is promoted by applying a certain load to muscle strength. It is strength training that is continued for 30 minutes to 5 hours at a frequency of 1 to 7 days in a week. Examples of strength training include dumbbell exercise, squat exercise, and machine training.
- Examples of subjects who take the muscle synthesis promoter of the present invention include, for example, athletes, sports enthusiasts (athletes), those who need exercise to improve lifestyle-related diseases, and elderly people for health promotion. Persons who need exercise, infants and / or children who need to build muscle during the development / growth process, or suffer from muscle lowering diseases (eg sarcopenia) and need to promote muscle synthesis through exercise And patients. Further, the target may be an animal other than a human (domestic animals such as horses and cows, pets such as dogs and cats, appreciation animals kept in zoos, etc.).
- the time when the muscle synthesis promoter of the present invention is ingested (administered) by a subject in need of immediate muscle synthesis promotion may be before exercise, after exercise, or during exercise.
- the time of intake is preferably after exercise, more preferably immediately after exercise.
- the intake period is preferably within 3 hours after the end of exercise, immediately after exercise. More preferably, within 2 hours after the end of exercise, more preferably within 1 hour after the end of exercise, and particularly preferably within 30 minutes after the end of exercise (Levenhagenhaet al. Am J Physiol Endocrinol Metab (2001), 280, EE982-E993).
- Immediately effective muscle synthesis promoting action equal to or better than whey protein means an action equal to or higher than the muscle synthesis promoting action observed when orally ingesting (administering) whey protein having the same protein content.
- the muscle synthesis promoting action includes an anabolic action of muscle protein from amino acids.
- the muscle synthesis promoting action can be confirmed, for example, by measuring the triceps surae muscle synthesis rate (FSR) of the upper limb in the test group within 1 hour after administration of the test substance after the rat swimming test. it can.
- FSR triceps surae muscle synthesis rate
- the FSR of the muscle synthesis promoter group of the present invention is comparable or similar to the FSR of the control group that orally ingested the same amount of whey protein, which is said to have an immediate effect of promoting muscle synthesis. In the case of the degree or more, it can be judged that the muscle synthesis promoting action is significant.
- Protein content means the total amount of amino acids contained in various proteins.
- the protein content may be measured and calculated by a conventional method such as Kjeldahl method or Raleigh method. Actually, in the case of the Kjeldahl method, it can be calculated by measuring nitrogen contained in various proteins and multiplying the value by a nitrogen-protein conversion factor (usually 6.25).
- a nitrogen-protein conversion factor usually 6.25.
- the whey protein hydrolyzate of the present invention is also called whey peptide, and there is no particular limitation on the means and / or method as long as all or part of the whey protein as a raw material is hydrolyzed.
- the whey protein used in the present invention may be a whey protein that has been separated or purified, and is a raw material, food or drink that already contains whey protein and casein protein in a specific protein ratio (for example, a milk protein mixture, etc.) ) Can also be used.
- the separated and purified whey proteins include, for example, whey stock solutions (sweet whey, acid whey, etc.), concentrates thereof, dried products (whey powder etc.), frozen products thereof, and reduced solutions thereof. Can be used. Furthermore, desalted whey, whey protein concentrate (WPC), whey protein purified product (WPI), ⁇ -lactalbumin ( ⁇ -La), ⁇ -lactoglobulin ( ⁇ -Lg), immunoglobulin, lactoferrin, etc., and These reducing solutions can be used.
- WPC whey protein concentrate
- WPI whey protein purified product
- ⁇ -La ⁇ -lactalbumin
- ⁇ -Lg ⁇ -lactoglobulin
- immunoglobulin lactoferrin, etc.
- raw materials and foods and drinks containing whey protein include raw milk, pasteurized milk (such as milk), skim milk, component-adjusted milk, processed milk, and dairy products (concentrated milk, powdered milk, condensed milk, fermented milk (yogurt) Etc.), lactic acid bacteria beverages, processed cheeses, ice creams, creams), and milk protein concentrates (MPC), and their concentrates, their dried products, their frozen products, and the like.
- the raw material and food / beverage products containing the said whey protein may acquire and use a commercial item, and may prepare and use them themselves.
- the whey protein hydrolyzate of the present invention can be prepared by hydrolyzing the whey protein as described above once or more.
- the whey protein may be hydrolyzed at least partially during the separation and purification steps, and after such partial hydrolysis, further hydrolysis may be performed.
- the whey protein hydrolyzate of the present invention can be prepared by hydrolyzing the whey protein by a known method, but can be preferably prepared by hydrolysis with a proteolytic enzyme.
- the protein hydrolase is not particularly limited as long as the effect of promoting muscle synthesis of the present invention can be obtained.
- the type and reaction conditions are arbitrarily set with reference to the description of Japanese Patent No. 5645360. it can.
- proteolytic enzymes derived from microorganisms belonging to the genus Bacillus or Aspergillus papain derived from papaya, bromelain derived from pineapple, actinidine derived from kiwi, pancreatin, trypsin
- proteolytic enzymes derived from animals such as chymotrypsin can be used alone or in combination.
- a commercial item can be used for these proteolytic enzymes.
- proteolytic enzymes include, for example, protease M Amano (Amanoenzyme), protease N Amano (Amanoenzyme), protease P Amano (Amanoenzyme), protease A Amano (Amanoenzyme), trypsin (Novo) ), Pepsin (Wako Pure Chemical Industries), Umamizyme (Amanoenzyme), and Flavorzyme (Novo), but are not limited thereto.
- the proteolytic enzyme used to hydrolyze whey protein is selected from the group consisting of Bacillus derived proteolytic enzyme, Aspergillus derived proteolytic enzyme, trypsin, pepsin, and flavorzyme. One kind or two or more kinds.
- the reaction temperature is preferably 20 to 80 ° C., more preferably 25 to 75 ° C., and still more preferably 30 It is ⁇ 70 ° C., more preferably 35 to 65 ° C., further preferably 40 to 60 ° C., particularly preferably 45 to 55 ° C.
- a reaction temperature of 20 ° C. or higher is preferable because whey protein can be efficiently hydrolyzed by a proteolytic enzyme.
- the reaction temperature is 80 ° C. or lower, inactivation due to denaturation of the proteolytic enzyme is suppressed, which is preferable.
- the reaction temperature when hydrolyzing whey protein with a protein hydrolase is one step in view of the optimum temperature of the protein hydrolase used, or Two or more reaction temperatures can be set. When two or more reaction temperatures are set, the order is arbitrary.
- the reaction time is preferably 2 to 48 hours, more preferably 2 to 36 hours, still more preferably 2 -24 hours, more preferably 2-18 hours, more preferably 2-14 hours, more preferably 4-12 hours, still more preferably 6-10 hours, particularly preferably 7-9 hours.
- a reaction time of 2 hours or longer is preferable because much whey protein can be hydrolyzed by a proteolytic enzyme.
- a reaction time of 48 hours or less is preferable because the whey protein can be efficiently hydrolyzed.
- the reaction time is arbitrarily determined in the above reaction time. Two or more reaction temperatures can be set.
- the amount of protein hydrolase added is particularly effective if the muscle synthesis promoting effect of the present invention is achieved.
- it is preferably 0.5 to 10 g, more preferably 0.7 to 5 g, still more preferably 0.8 to 4 g, and particularly preferably 1 to 2 g with respect to 100 g of protein.
- the reaction pH when hydrolyzing the whey protein with a protein hydrolase is preferably near the optimum pH of the protein hydrolase used.
- a proteolytic enzyme derived from the genus Bacillus and a proteolytic enzyme derived from the genus Aspergillus are used in combination, it is preferably pH 5-9, more preferably pH 5.5-8.5, still more preferably pH 6-8, and particularly preferably Has a pH of 6.5 to 7.5.
- reaction pH is one step in view of the optimum pH of the protein hydrolase used, or Two or more stages of reaction pH can be set.
- reaction pH of two or more steps the order is arbitrary.
- the whey protein hydrolyzate of the present invention can also be used as an indicator that a specific dipeptide is contained as an indicator of degradation.
- the specific dipeptide is preferably Ile-Leu, Leu-Ala, Ala-Leu, Val-Leu, Lys-Ile, Leu-Leu, Ile-Asn, Leu-Glu, Leu-Val, and Ile-Val, which includes at least one dipeptide selected from these groups.
- Ile is isoleucine
- Leu is leucine
- Ala is alanine
- Val is valine
- Lys is lysine
- Asn is asparagine
- Glu glutamic acid.
- the dipeptide can be measured by LC / MS, and specifically can be measured by the method described in Test Example 1 described later.
- the specific dipeptides preferably includes a specific dipeptide that satisfies the following (1) and (2).
- (1) Ile-Leu and Leu-Ala, and (2) Ala-Leu, Val-Leu, Lys-Ile, Leu-Leu, Ile-Asn, Leu-Glu, Leu-Val, and Ile-Val At least one dipeptide selected from the group; That is, it is particularly preferred that (1) Ile-Leu and Leu-Ala are each contained in the whey protein hydrolyzate of the present invention, and (2) Ala-Leu, Val-Leu, Lys-Ile. It is particularly preferable that at least one dipeptide among Leu-Leu, Ile-Asn, Leu-Glu, Leu-Val, and Ile-Val is contained in the whey protein hydrolyzate of the present invention.
- the content is preferably the protein content of the whey protein hydrolyzate (before hydrolysis).
- the protein content of whey protein) is 0.5 mg or more per gram, more preferably 0.5 to 10 mg per gram of protein content, still more preferably 0.5 to 8 mg per gram of protein content, and still more preferably 0.5 per gram of protein content. -6 mg, particularly preferably 0.5-4 mg per gram of protein content.
- the muscle synthesis promoting effect of the present invention is more exhibited, which is preferable.
- the content of the dipeptide Ile-Leu is 10 mg or less per gram of protein content of the whey protein hydrolyzate, the whey protein hydrolyzate of the present invention can be efficiently prepared, which is preferable.
- the content thereof is preferably the protein content of whey protein hydrolyzate (before hydrolysis).
- the protein content of whey protein) is 0.5 mg or more per gram, more preferably 0.5 to 10 mg per gram of protein content, still more preferably 0.5 to 8 mg per gram of protein content, and still more preferably 0.5 per gram of protein content. -6 mg, particularly preferably 0.5-4 mg per gram of protein content.
- the muscle synthesis promoting effect of the present invention is more exhibited, which is preferable.
- the content of the dipeptide Leu-Ala is 10 mg or less per gram of protein content of the whey protein hydrolyzate, the whey protein hydrolyzate of the present invention can be efficiently prepared, which is preferable.
- the content is preferably the protein content of whey protein hydrolyzate (before hydrolysis).
- the protein content of whey protein) is 0.2 mg or more per gram, more preferably 0.2 to 10 mg per gram of protein content, still more preferably 0.2 to 8 mg per gram of protein content, still more preferably 0.2 per gram of protein content. -6 mg, particularly preferably 0.2-5 mg per gram of protein content. If the content of the dipeptide Ala-Leu is 0.2 mg or more per gram of protein content of the whey protein hydrolyzate, the muscle synthesis promoting effect of the present invention is more exhibited, which is preferable. Moreover, if the content of the dipeptide Ala-Leu is 10 mg or less per gram of protein content of the whey protein hydrolyzate, the whey protein hydrolyzate of the present invention can be efficiently prepared, which is preferable.
- the content thereof is preferably the protein content of whey protein hydrolyzate (before hydrolysis).
- the protein content of whey protein is 1 mg or more per gram, more preferably 1 to 15 mg per gram of protein content, more preferably 1 to 12 mg per gram of protein content, still more preferably 1 to 10 mg per gram of protein content, particularly preferably the protein content 1-8 mg per gram. If the content of the dipeptide Val-Leu is 1 mg or more per gram of protein content of the whey protein hydrolyzate, the muscle synthesis promoting effect of the present invention is more exhibited, which is preferable. Moreover, if the content of dipeptide Val-Leu is 15 mg or less per gram of protein content of the whey protein hydrolyzate, the whey protein hydrolyzate of the present invention can be efficiently prepared, which is preferable.
- the content is preferably the protein content of the whey protein hydrolyzate (before hydrolysis).
- the protein content of whey protein) is 0.2 mg or more per gram, more preferably 0.2 to 15 mg per gram of protein content, still more preferably 0.2 to 12 mg per gram of protein content, still more preferably 0.2 per gram of protein content. ⁇ 10 mg, particularly preferably 0.2 to 8 mg / g protein content.
- the muscle synthesis promoting effect of the present invention is more exhibited, which is preferable. Further, if the content of the dipeptide Lys-Ile is 15 mg or less per gram of protein content of the whey protein hydrolyzate, the whey protein hydrolyzate of the present invention can be efficiently prepared, which is preferable.
- the content is preferably the protein content of the whey protein hydrolyzate (before hydrolysis).
- the protein content of whey protein) is 0.2 mg or more per gram, more preferably 0.2 to 25 mg per gram of protein content, still more preferably 0.2 to 20 mg per gram of protein content, still more preferably 0.2 per gram of protein content. ⁇ 18 mg, particularly preferably 0.2 to 16 mg / g protein content.
- the muscle synthesis promoting effect of the present invention is more exhibited, which is preferable.
- the content of the dipeptide Leu-Leu is 25 mg or less per gram of protein content of the whey protein hydrolyzate, the whey protein hydrolyzate of the present invention can be efficiently prepared, which is preferable.
- the content is preferably the protein content of the whey protein hydrolyzate (before hydrolysis).
- the protein content of whey protein) is 1 mg or more per gram, more preferably 1 to 10 mg per gram of protein content, more preferably 1 to 8 mg per gram of protein content, more preferably 1 to 6 mg per gram of protein content, particularly preferably the protein content 1 to 5 mg per gram. If the content of the dipeptide Ile-Asn is 1 mg or more per gram of protein content of the whey protein hydrolyzate, the muscle synthesis promoting effect of the present invention is more exhibited, which is preferable. Moreover, if the content of the dipeptide Ile-Asn is 10 mg or less per gram of protein content of the whey protein hydrolyzate, the whey protein hydrolyzate of the present invention can be efficiently prepared, which is preferable.
- the content thereof is preferably the protein content of whey protein hydrolyzate (before hydrolysis).
- the protein content of whey protein) is 0.5 mg or more per gram, more preferably 0.5 to 12 mg per gram of protein content, still more preferably 0.5 to 10 mg per gram of protein content, more preferably 0.5 per gram of protein content. ⁇ 8 mg, particularly preferably 0.5 to 6 mg per g protein content. If the content of the dipeptide Leu-Glu is 0.5 mg or more per gram of protein content of the whey protein hydrolyzate, the muscle synthesis promoting effect of the present invention is more exhibited, which is preferable. Moreover, if the content of the dipeptide Leu-Glu is 12 mg or less per gram of protein content of the whey protein hydrolyzate, the whey protein hydrolyzate of the present invention can be efficiently prepared, which is preferable.
- the whey protein hydrolyzate of the present invention is in a liquid state immediately after preparation, in order to enhance its storage stability, it is cryopreserved, dried (spray-dried, freeze-dried), concentrated, and heat-sterilized (plate-type heat-sterilized, tube -Type heat sterilization, batch-type heat sterilization, current heat sterilization, microwave heat sterilization, indirect heat sterilization such as retort heating, direct heat sterilization such as steam injection and steam infusion), non-heat sterilization (light irradiation sterilization, radiation Irradiation sterilization, high voltage pulse sterilization).
- the form dried (spray drying, freeze-drying) is preferable from the ease of handling.
- the whey protein hydrolyzate of the present invention can enhance the muscle synthesis promoting effect as compared with the whey protein itself, which is the effect of the present invention
- other food ingredients and / or food additive ingredients may be added.
- it can.
- sweeteners sucgars, liquid sugars, fructose, maltose, trithermal sugar, etc.
- sugar alcohols erythritol, etc.
- high-sensitivity sweeteners stevia
- Aspartame, sucralose, acesulfame potassium acidulant, thickening polysaccharide, fragrance, fruit juice, emulsifier, vegetable juice, and the like.
- the muscle synthesis promoter of the present invention can be used for the purpose of imparting additional functions of the muscle synthesis promoter of the present invention.
- minerals calcium, iron, manganese, magnesium, zinc, etc.
- vitamins, functional materials, probiotic lactic acid bacteria, etc. are added,
- the muscle synthesis promoter of the present invention can be used.
- the muscle synthesis promoter of the present invention can be in the form of a powder in a container for the purpose of providing in the market.
- a well-known thing can be used for a container, such as paper, a bag, a box, a can, and a bottle.
- the capacity of the container is not particularly limited, and for example, 1 to 5000 g, 2 to 4000 g, 3 to 3000 g, 4 to 2000 g, 4 to 1000 g, 4 to 500 g, 4 to 250 g, 4 to 200 g, 4 to 100 g, 4 to 100 g, 50 g, 4-30 g.
- a family size for example, 300 to 5000 g, 300 to 4000 g, 300 to 3000 g, 300 to 2000 g, 300 to 1500 g, 300 to 1400 g, 300 to 1300 g, 300 to 1200 g, 300 to 1100 g.
- personal size 1 to 500 g, 2 to 250 g, 3 to 200 g, 4 to 100 g, 4 to 50 g, 4 to 40 g, 4 to 30 g, 5 to 30 g, 10 to 30 g, 15 to 30 g.
- the muscle synthesis promoter of the present invention can be in the form of a beverage in a container (a beverage in which powder is dispersed and dissolved) for the purpose of providing on the market.
- a container such as a paper container, a soft bag, a PET bottle, a can, and a bottle.
- the capacity of the container is not particularly limited, and is, for example, 1 to 5000 g, 2 to 4000 g, 3 to 3000 g, 4 to 2000 g, 5 to 1000 g, 6 to 800 g, 7 to 1300 g, 80 to 1200 g, 90 to 1100 g.
- a family size for example, 300 to 5000 g, 300 to 4000 g, 300 to 3000 g, 300 to 2000 g, 300 to 1500 g, 300 to 1400 g, 300 to 1300 g, 300 to 1200 g, 300 to 1100 g
- it is 10 to 500 g, 20 to 500 g, 30 to 500 g, 40 to 500 g, 50 to 500 g, 60 to 500 g, 70 to 500 g, 80 to 500 g, 90 to 500 g.
- ingestion such that the protein content of the whey protein degradation product is preferably 0.05 g / kg body weight or more per meal. More preferably 0.05 to 0.67 g / kg body weight, still more preferably 0.05 to 0.33 g / kg body weight, more preferably 0.07 to 0.33 g / kg body weight, still more preferably It is ingested (administered) so as to be 0.07 to 0.25 g / kg body weight, particularly preferably 0.08 to 0.25 g / kg body weight.
- the typical weight of the subject is estimated to be about 60 kg.
- one meal means one time in a state that requires immediate effective muscle synthesis promotion.
- a single meal is not taken at a time (at a time), It may be taken in several degrees.
- the muscle synthesis promoting agent of the present invention is ingested (administered) to a subject in need of immediate muscle synthesis promotion, the amino acid concentration in the blood stream can be immediately In terms of improvement, it is preferably within 2 hours after the end of exercise, more preferably within 1 hour after the end of exercise, more preferably within 30 minutes after the end of exercise, and particularly preferably, Within 5 minutes after the end of exercise.
- the method of ingesting (administering) the muscle synthesis promoting agent of the present invention to a subject in need of immediate muscle synthesis promotion is based on oral administration (oral administration), enteral administration, gastric fistula, etc. Can be appropriately selected, but is preferably taken orally.
- the muscle synthesis promoter of the present invention comprises a unit package form per serving, and the whey protein degradation product is contained in the unit as the total intake of the protein.
- the content is preferably adjusted to 1 g or more in terms of content, specifically 1 to 40 g, preferably 2 to 20 g, more preferably 3 to 20 g, further preferably 4 to 15 g, particularly preferably 5 to 15 g. To be prepared.
- ⁇ consisting of a unit packaging form per meal '' is a form in which the intake amount per meal is determined in advance, for example, as a food and drink that can be taken orally in a specific amount, not only general foods, It means forms such as beverages (drinks, etc.), health supplements, health functional foods, and supplements.
- unit packaging form per meal for example, in the case of liquid beverages, gel-like, paste-like, paste-like jelly, powder-like, granule-like, capsule-like, block-like solid foods, etc.
- a specific amount (dose) can be defined in packaging containers such as cans, glass bottles (such as bottles), plastic containers (such as plastic bottles), packs, pouches, film containers, paper boxes, etc., or intake per serving (usage, dose) ) Is displayed on a packaging container, a homepage, etc., and the form which can prescribe
- the muscle synthesis promoter of the present invention has the effect of the present invention even if it is ingested in a small amount, so it is in any form such as a bite-sized block shape or a mini can drink that can be consumed with a single drink.
- intake and swallowing are easy, and convenience for children and elderly people can be improved.
- the muscle synthesis promoter of the present invention is such that the whey protein hydrolyzate is Ile-Leu, Leu-Ala, Ala-Leu, Val-Leu, Lys-Ile, Leu-Leu, Ile. -Includes dipeptides consisting of Asn, Leu-Glu, Leu-Val, and Ile-Val.
- the muscle synthesis promoter of the present invention is such that the whey protein hydrolyzate is Ile-Leu, Leu-Ala, Val-Leu, Lys-Ile, Leu-Leu, Ile-Asn, It includes dipeptides consisting of Leu-Glu, Leu-Val, and Ile-Val.
- the muscle synthesis promoter of the present invention can provide a composition comprising a muscle synthesis promoter. That is, the muscle synthesis promoter of the present invention can be used alone as it is, but as long as the function of promoting muscle synthesis is exerted, various compositions for oral intake (for oral administration) such as foods and drinks and pharmaceuticals.
- the composition can also be included as a raw material (raw material), an additive and the like, and a composition having an immediate effect of promoting muscle synthesis can be obtained. Therefore, according to one embodiment of the present invention, a composition comprising the muscle synthesis promoter of the present invention is provided.
- the muscle synthesis promoter of the present invention can itself be provided in the form of a composition containing a plurality of components. Therefore, according to one embodiment of the present invention, there is provided a composition for promoting muscle synthesis, comprising a whey protein hydrolyzate as an active ingredient.
- a composition for promoting muscle synthesis comprising a whey protein hydrolyzate as an active ingredient.
- the form of these compositions is not particularly limited as long as it exerts an effect of promoting muscle synthesis.
- the composition is solid (powder, granule, capsule, block, etc.), liquid, gel, paste, It can take a form such as a paste, and is preferably a powder.
- Compositions include food and beverage compositions and pharmaceutical compositions.
- a food and drink for promoting muscle synthesis comprising whey protein hydrolyzate as an active ingredient.
- the form of the food or drink is not particularly limited as long as it is a form that can be taken orally, such as a solution, suspension, emulsion, powder, or solid molded product.
- soft drink, fruit juice drink, vegetable drink, soy milk drink, coffee drink, tea Beverages such as beverages, powdered beverages, concentrated beverages, alcoholic beverages
- flour products such as bread, pasta, noodles, cake mixes, bread crumbs
- milk drinks, yogurts, lactic acid bacteria drinks, ice creams, creams How dairy products; agricultural canning, jam, marmalade such, processed agricultural products such as serial; such as frozen food, and the like.
- the food or drink is a dairy product, more preferably
- Foods and drinks include health foods, functional foods, dietary supplements, functional labeling foods, foods for specified health use, foods for the sick, infant formulas, milk powders for pregnant or lactating women, or muscle synthesis promotion
- foods and beverages with a label indicating that they are used are included.
- a pharmaceutical product for promoting muscle synthesis which contains a whey protein hydrolyzate as an active ingredient.
- the pharmaceutical may be prepared as an oral preparation or a parenteral preparation according to a conventional method using additives that are acceptable for formulation.
- oral preparations take the form of solid preparations such as tablets, powders, fine granules, granules, capsules, pills, sustained-release preparations, and liquid preparations such as solutions, suspensions, and emulsions. Can do.
- a parenteral preparation it can take the form of an injection or a suppository. From the viewpoint of simplicity, an oral preparation is preferable.
- Additives that are acceptable for formulation include, for example, excipients, stabilizers, preservatives, wetting agents, emulsifiers, lubricants, sweeteners, colorants, fragrances, buffers, antioxidants, pH Examples thereof include regulators.
- the pharmaceutical agent of the present invention can be used for the treatment or prevention of diseases that require muscle synthesis promotion, specifically, muscle damage diseases (for example, sarcopenia).
- the muscle synthesis promoter of the present invention can be provided by blending an effective amount of whey protein hydrolyzate and any orally acceptable additive. Therefore, according to another aspect of the present invention, there is provided a method for producing a muscle synthesis promoter comprising blending an effective amount of a whey protein hydrolyzate.
- the method for producing a muscle synthesis promoter of the present invention may further include a step of heating and homogenizing a mixture obtained by blending the whey protein hydrolyzate, and the present invention includes such an embodiment.
- a method for promoting muscle synthesis comprising ingesting an effective amount of a whey protein hydrolyzate to a subject in need thereof.
- the method excludes medical practice for humans.
- the “medical action for humans” means an action that requires a doctor or the like to ingest (administer) a medicine to a human.
- the subject is preferably a healthy person.
- the muscle synthesis promotion method of the present invention may be used to improve a disease or condition related to muscle damage in a subject. Therefore, the method for promoting muscle synthesis of the present invention is preferably a method for treating a disease or condition associated with muscle damage, and more preferably a method for improving sarcopair. As used herein, “amelioration” includes not only treating an established disease or condition, but also preventing a disease or condition that may be established in the future. In addition, the muscle synthesis promotion method of this invention can be implemented according to description of the muscle synthesis promoter in this specification.
- ingestion such that the protein content of the whey protein degradation product is preferably 0.05 g / kg body weight or more per meal. More preferably 0.05 to 0.67 g / kg body weight, still more preferably 0.05 to 0.33 g / kg body weight, more preferably 0.07 to 0.33 g / kg body weight, still more preferably 0. 0.07 to 0.25 g / kg body weight, particularly preferably 0.08 to 0.25 g / kg body weight.
- the typical weight of the subject is estimated to be about 60 kg.
- a sufficient effect can be obtained even when the intake dose is small as compared with the case where the whey protein is ingested, so that the burden on the child and the elderly can be reduced.
- the whey protein hydrolyzate in the muscle synthesis promoting method of the present invention, can be ingested so that the protein content is 1 g or more per meal, specifically,
- the ingestion amount can be 1 to 40 g, preferably 2 to 20 g, more preferably 3 to 20 g, still more preferably 4 to 15 g, and particularly preferably 5 to 15 g.
- the use of a whey protein hydrolyzate for promoting muscle synthesis is provided.
- the use of the invention is a non-therapeutic use.
- a whey protein hydrolyzate for the production of a food or drink for promoting muscle synthesis or a pharmaceutical product.
- a whey protein hydrolyzate for promoting muscle synthesis is provided.
- the effect of promoting muscle synthesis can be further enhanced as compared with the unhydrolyzed whey protein itself.
- the muscle synthesis promoter of the present invention is higher in the intake (administration) of a certain amount of protein content than the whey protein not hydrolyzed. It was shown to have an effect.
- the muscle synthesis promoter of the present invention was ingested (administered) at 0.5 g protein / kg body weight, whey protein was 2.0 g protein / kg body weight. Compared to the case of ingestion (administration), the effect as a muscle synthesis promoter was higher than the equivalent.
- the muscle synthesis promoting agent of the present invention has a strong muscle synthesis promoting ability when a certain amount of protein content is ingested (administered) as compared with the whey protein conventionally known for its rapid muscle synthesis promoting effect.
- the effect can be expected.
- the muscle synthesis promoter of the present invention can reduce the protein content to be ingested when obtaining an effect equivalent to the intake of whey protein, and can be expected to be effective in a small amount. It becomes easy to do.
- the whey protein hydrolyzate which is an active ingredient has already been partially decomposed, the digestive burden can be reduced.
- whey protein hydrolyzate of the present invention can exert the effect of promoting muscle synthesis with a smaller amount of intake than whey protein.
- Protein in “mg / g protein” and “g protein / kg body weight” described later means the protein content.
- Example 1 50 g of whey protein was dissolved in 1 L of water.
- the dissolved whey protein solution is adjusted to pH 7 with a pH adjusting agent, and combined with 500 mg of protease derived from Bacillus (Protease M Amano, Amano Enzyme) and 500 mg of protease derived from Aspergillus (Protease N Amano, Amano Enzyme)
- the whey protein was hydrolyzed at 50 ° C. for 8 hours. After the protease was deactivated by heating, the resulting solution was powdered by freeze-drying and used as the muscle synthesis promoter of Example 1.
- Example 1 The muscle synthesis promoter powder of Example 1 was diluted 1000 times (volume ratio) in a 0.1% trifluoroacetic acid aqueous solution, and LC / MS was used under the following conditions to obtain Ile-Leu, Ile-Trp, Ala- Leu, Val-Leu, Asp-Leu, Lys-Ile, Leu-Leu, Ile-Asn, Leu-Ala, and Leu-Glu were quantified (unit: mg / g protein).
- the LC / MS conditions are as follows.
- the muscle synthesis promoter Ile-Leu of Example 1 was 3.82 mg / g protein, Ala-Leu was 1.53 mg / g protein, Val-Leu was 4.70 mg / g protein, and Lys-Ile was 4.82 mg / g.
- the g protein, Leu-Leu was 1.56 mg / g protein, Ile-Asn was 3.98 mg / g protein, Leu-Ala was 0.75 mg / g protein, and Leu-Glu was 4.35 mg / g protein.
- Example 2 The muscle synthesis promoting effect of the muscle synthesis promoter of Example 1 was verified as follows.
- Rat swimming test SD male rats obtained from CLEA Japan, Inc. having an initial weight of 130 to 170 g were used as experimental animals.
- the rats were preliminarily raised for 3 to 5 days using a solid feed (Oriental Yeast Co., Ltd.), and then subjected to a 30-minute swimming exercise (water temperature: 34 ° C.) in order to acclimate to the swimming exercise. After fasting for 16 hours from the next day to the day after the acclimated swimming exercise, the rats were divided into five groups (8 in each test group) so that there was no difference in body weight between the test groups.
- the rats were subjected to a 2-hour swimming exercise.
- 20 mL / kg of water ie 0 g protein / kg body weight
- a muscle synthesis promoter 2.5% or 10% by weight solution or whey protein 2.5% or 10% by weight solution
- Rats were orally ingested at body weight (ie 0.5 g protein / kg body weight or 2.0 g protein / kg body weight).
- a deuterium labeled phenylalanine solution (prepared by dissolving 225 mg of deuterium labeled phenylalanine in 10 mL of physiological saline) was administered to the rat via the tail vein at 2.0 mL / kg body weight.
- Rats were dissected under isoflurane anesthesia 15 minutes after tail vein administration (ie 60 minutes after oral ingestion). After whole blood was collected from the abdominal vena cava, the triceps muscle of the upper limb was removed, washed with physiological saline, and immediately frozen with liquid nitrogen. The time of tail vein administration and the time of cryopreservation of the upper limb triceps were accurately recorded. The upper limb triceps were stored at ⁇ 80 ° C. until analysis.
- apparatus LC / MS / MS (manufactured by Waters), column: ACQUITY UPLC BEH C18 (manufactured by Waters), mobile phase: acetonitrile containing 0.05% TFA water / 0.05% TFA, injection amount 3 ⁇ L, flow rate 0.3 mL / min, UV 215 nm.
- FSR skeletal muscle synthesis rate
- the average value ⁇ standard error of each test group was determined from the obtained numerical values.
- a water intake group (0 g protein / kg body weight) was used as a control group, and a multiple comparison test by the Dunnett method was performed in all groups.
- Student's t-test was performed between the group ingesting the muscle synthesis promoter of Example 1 and the group ingested whey protein at the same protein intake. In all cases, the significance level was less than 5% (p ⁇ 0.05). The result is shown in FIG.
- Example 1 This muscle synthesis promoter significantly increased the rate of skeletal muscle synthesis in rats compared to the case of water ingestion at both 0.5 g protein / kg body weight and 2.0 g protein / kg body weight ( FIG. 1). Furthermore, the rate of skeletal muscle synthesis in rats after ingesting 0.5 g protein / kg body weight of the muscle synthesis promoter of Example 1 was compared with the rate of skeletal muscle synthesis in rats after ingesting 0.5 g protein / kg body weight of whey protein. The value was significantly high (FIG. 1).
- the rate of skeletal muscle synthesis in rats when 2.0 g protein / kg body weight of the muscle synthesis promoter of Example 1 was ingested was the same as that of whey protein having the same protein content. Compared to speed, it was on an upward trend from the equivalent (Fig. 1).
- the skeletal muscle synthesis rate of rats after ingesting 0.5 g protein / kg body weight of the muscle synthesis promoter of Example 1 was also compared with the skeletal muscle synthesis rate of rats after ingesting 2.0 g protein / kg body weight of whey protein. As a result, there was an upward trend from the equivalent (FIG. 1).
- the muscle synthesis promoter of the present invention has a higher muscle synthesis promoting effect than whey protein, and it was confirmed that the difference between these effects becomes remarkable particularly when ingested in small amounts.
- the muscle synthesis promoter of the present invention has been known to have an immediate effect of promoting muscle synthesis, and exerts an equivalent muscle synthesis promoting effect when consumed in a smaller amount than whey protein. Moreover, it has been found that the muscle synthesis promoter of the present invention retains an excellent muscle synthesis promoting action and can reduce the amount of intake even though the hydrolyzate is an active ingredient. From this, it is confirmed that the muscle synthesis promoter of the present invention is advantageous in effectively promoting muscle synthesis while reducing the digestive burden of subjects with reduced visceral functions such as kidney and stomach. It was done.
- Example 2 50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution was adjusted to pH 7 with a pH adjusting agent, 500 mg of protease derived from Bacillus (Protease M Amano, Amano Enzyme) was added to the solution, and whey protein was hydrolyzed at 50 ° C. for 8 hours. After the protease was inactivated by heating, the resulting solution was powdered by freeze-drying and used as the muscle synthesis promoter of Example 2.
- protease derived from Bacillus Protease M Amano, Amano Enzyme
- the muscle synthesis promoter Ile-Leu of Example 2 was 2.39 mg / g protein, Ala-Leu was 0.64 mg / g protein, Val-Leu was 2.35 mg / g protein, and Lys-Ile was 1.97 mg / g.
- the g protein, Leu-Leu was 0.67 mg / g protein, Ile-Asn was 2.73 mg / g protein, Leu-Ala was 1.22 mg / g protein, and Leu-Glu was 1.90 mg / g protein.
- Example 3 50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution was adjusted to pH 7 with a pH adjusting agent, 500 mg of protease derived from Aspergillus (Protease N Amano, Amano Enzyme) was added to the solution, and whey protein was hydrolyzed at 50 ° C. for 8 hours. After the protease was inactivated by heating, the resulting solution was powdered by freeze-drying and used as the muscle synthesis promoter of Example 3.
- protease derived from Aspergillus Protease N Amano, Amano Enzyme
- the muscle synthesis promoter Ile-Leu of Example 3 was 3.74 mg / g protein, Ala-Leu was 1.98 mg / g protein, Val-Leu was 2.13 mg / g protein, and Lys-Ile was 1.07 mg / g.
- the g protein, Leu-Leu was 8.20 mg / g protein, Ile-Asn was 2.40 mg / g protein, Leu-Ala was 1.77 mg / g protein, and Leu-Glu was 1.08 mg / g protein.
- Example 4 50 g of whey protein was dissolved in 1 L of water.
- the dissolved whey protein solution is adjusted to pH 7 with a pH adjusting agent, 500 mg of trypsin (Novo) is added to the solution, and after reacting at 37 ° C. for 4 hours, 500 mg of protease derived from Bacillus (protease M Amano, Amano Enzyme) is added.
- the whey protein was hydrolyzed at 50 ° C. for 4 hours. After the protease was inactivated by heating, the resulting solution was powdered by freeze-drying and used as the muscle synthesis promoter of Example 4.
- the muscle synthesis promoter Ile-Leu of Example 2 was 3.16 mg / g protein, Ala-Leu was 4.77 mg / g protein, Val-Leu was 5.33 mg / g protein, and Lys-Ile was 4.84 mg / g.
- the g protein, Leu-Leu was 1.07 mg / g protein, Ile-Asn was 1.65 mg / g protein, Leu-Ala was 0.83 mg / g protein, and Leu-Glu was 4.82 mg / g protein.
- Example 5 50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution was adjusted to pH 7 with a pH adjusting agent, 500 mg of trypsin (Novo) was added to the solution, reacted at 37 ° C. for 4 hours, and then protease derived from the genus Aspergillus (Protease N Amano, Amano Enzyme), 50 The whey protein was hydrolyzed at 4 ° C. for 4 hours. After the protease was deactivated by heating, the resulting solution was powdered by freeze-drying and used as the muscle synthesis promoter of Example 5.
- trypsin Novo
- the muscle synthesis promoter Ile-Leu of Example 5 is 3.57 mg / g protein, Ala-Leu is 2.04 mg / g protein, Val-Leu is 2.60 mg / g protein, and Lys-Ile is 0.74 mg / g.
- the g protein, Leu-Leu was 4.89 mg / g protein, Ile-Asn was 1.61 mg / g protein, Leu-Ala was 1.96 mg / g protein, and Leu-Glu was 0.71 mg / g protein.
- Example 6 50 g of whey protein was dissolved in 1 L of water.
- the dissolved whey protein solution is adjusted to pH 7 with a pH adjusting agent, 500 mg of pepsin (Wako Pure Chemical Industries, Ltd.) is added to the solution, and after reacting at 37 ° C. for 4 hours, a protease derived from Bacillus (protease M Amano, Amano Enzyme) 500 mg was added to the solution and the whey protein was hydrolyzed at 50 ° C. for 4 hours. After the protease was inactivated by heating, the resulting solution was powdered by freeze-drying and used as the muscle synthesis promoter of Example 6.
- the muscle synthesis promoter Ile-Leu of Example 6 was 1.47 mg / g protein, Ala-Leu was 0.75 mg / g protein, Val-Leu was 4.92 mg / g protein, and Lys-Ile was 0.54 mg / g.
- the g protein, Leu-Leu was 2.21 mg / g protein, Ile-Asn was 1.59 mg / g protein, Leu-Ala was 2.80 mg / g protein, and Leu-Glu was 0.62 mg / g protein.
- Example 7 50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution is adjusted to pH 7 with a pH adjusting agent, 500 mg of pepsin (Wako Pure Chemical Industries, Ltd.) is added to the solution, reacted at 37 ° C. for 4 hours, and then protease derived from the genus Aspergillus (protease N Amano, Amano Enzyme) The whey protein was hydrolyzed at 50 ° C. for 4 hours. After the protease was inactivated by heating, the resulting solution was powdered by freeze-drying and used as the muscle synthesis promoter of Example 7.
- pepsin Wipsin derived from the genus Aspergillus
- the muscle synthesis promoter Ile-Leu of Example 7 was 2.86 mg / g protein, Ala-Leu was 2.28 mg / g protein, Val-Leu was 3.90 mg / g protein, and Lys-Ile was 0.81 mg / g.
- the g protein, Leu-Leu was 13.37 mg / g protein, Ile-Asn was 2.82 mg / g protein, Leu-Ala was 3.08 mg / g protein, and Leu-Glu was 0.97 mg / g protein.
- Example 8 50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution was adjusted to pH 7 with a pH adjuster, and 500 mg of flavorzyme (Novo) was added to the solution, and the whey protein was hydrolyzed at 50 ° C. for 6 hours. After the protease was deactivated by heating, the resulting solution was powdered by freeze-drying and used as the muscle synthesis promoter of Example 8.
- the muscle synthesis promoter Ile-Leu of Example 8 was 0.62 mg / g protein, Ala-Leu was 0.35 mg / g protein, Val-Leu was 1.58 mg / g protein, and Lys-Ile was 1.54 mg / g.
- the g protein, Leu-Leu was 0.20 mg / g protein, Ile-Asn was 1.64 mg / g protein, Leu-Ala was 1.05 mg / g protein, and Leu-Glu was 1.40 mg / g protein.
- Example 9 50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution was adjusted to pH 7 with a pH adjuster, 500 mg of protease derived from the genus Aspergillus (Ummamizyme, Amano Enzyme) was added to the solution, and whey protein was hydrolyzed at 50 ° C. for 6 hours. After the protease was inactivated by heating, the resulting solution was powdered by freeze-drying and used as the muscle synthesis promoter of Example 9.
- protease derived from the genus Aspergillus Ummamizyme, Amano Enzyme
- the muscle synthesis promoter Ile-Leu of Example 3 was 2.78 mg / g protein, Ala-Leu was 1.09 mg / g protein, Val-Leu was 6.25 mg / g protein, and Lys-Ile was 6.45 mg / g.
- the g protein, Leu-Leu was 1.85 mg / g protein, Ile-Asn was 4.93 mg / g protein, Leu-Ala was 3.93 mg / g protein, and Leu-Glu was 5.87 mg / g protein.
- Example 10 50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution was adjusted to pH 7 with a pH adjuster, 500 mg of protease derived from Aspergillus (Protease A Amano, Amano Enzyme) was added to the solution, and whey protein was hydrolyzed at 50 ° C. for 6 hours. After heating and deactivating the protease, the resulting solution was powdered by freeze-drying and used as the muscle synthesis promoter of Example 10.
- protease derived from Aspergillus Protease A Amano, Amano Enzyme
- the muscle synthesis promoter Ile-Leu of Example 8 was 2.17 mg / g protein, Ala-Leu was 0.64 mg / g protein, Val-Leu was 4.23 mg / g protein, and Lys-Ile was 2.72 mg / g.
- the g protein, Leu-Leu was 2.22 mg / g protein, Ile-Asn was 3.23 mg / g protein, Leu-Ala was 2.97 mg / g protein, and Leu-Glu was 2.48 mg / g protein.
- Example 11 50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution was adjusted to pH 7 with a pH adjusting agent, 500 mg of protease derived from the genus Aspergillus (protease P Amano, Amano Enzyme) was added to the solution, and whey protein was hydrolyzed at 50 ° C. for 6 hours. After the protease was inactivated by heating, the resulting solution was powdered by freeze-drying and used as the muscle synthesis promoter of Example 11.
- protease derived from the genus Aspergillus protease derived from the genus Aspergillus
- the muscle synthesis promoter Ile-Leu of Example 3 was 1.85 mg / g protein, Ala-Leu was 0.66 mg / g protein, Val-Leu was 5.19 mg / g protein, and Lys-Ile was 3.83 mg / g.
- the g protein, Leu-Leu was 1.74 mg / g protein, Ile-Asn was 3.70 mg / g protein, Leu-Ala was 3.54 mg / g protein, and Leu-Glu was 3.56 mg / g protein.
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Abstract
Description
[1]ホエイタンパク質加水分解物を有効成分とする筋肉合成促進剤、
[2]ホエイタンパク質加水分解物が、ジペプチドを含み、前記ジペプチドが、
(1)Ile-Leu、及びLeu-Ala、並びに
(2)Ala-Leu、Val-Leu、Lys-Ile、Leu-Leu、Ile-Asn、Leu-Glu、Leu-Val、及びIle-Valよりなる群から選択される少なくとも1種のジペプチドである、[1]に記載の筋肉合成促進剤、
[3]ホエイタンパク質加水分解物が、ホエイタンパク質をタンパク質分解酵素により加水分解することにより得られる、[1]又は[2]に記載の筋肉合成促進剤、
[4]タンパク質分解酵素がバシラス属由来タンパク質分解酵素、アスペルギルス属由来タンパク質分解酵素、トリプシン、ペプシン、フレーバザイムより選ばれる1種又は2種以上である、[3]に記載の筋肉合成促進剤、
[5]タンパク質分解酵素による加水分解が、20~80℃で2~48時間で行われる、[3]又は[4]に記載の筋肉合成促進剤、
[6]一食あたりの単位包装形態からなり、該単位包装形態中に、ホエイタンパク質加水分解物を一食摂取量として総タンパク質含量換算で1~40g含む、[1]~[5]のいずれか1に記載の筋肉合成促進剤、
[7]運動する対象のための、[1]~[6]のいずれか1に記載の筋肉合成促進剤、
[8]運動が筋肉トレーニングである、[7]に記載の筋肉合成促進剤、
[9]筋肉合成促進用組成物である、[1]~[8]のいずれか一項に記載の剤、
[10]飲食品または医薬品である、[1]~[9]のいずれか一項に記載の剤。
[11]ホエイタンパク質加水分解物の有効量を配合すること含む、筋肉合成促進剤の製造方法。
[12]ホエイタンパク質加水分解物の有効量を、それを必要とする対象に摂取させることを含む、筋肉合成促進方法、
[13]ホエイタンパク質加水分解物が、ジペプチドを含み、前記ジペプチドが、
(1)Ile-Leu、及びLeu-Ala、並びに
(2)Ala-Leu、Val-Leu、Lys-Ile、Leu-Leu、Ile-Asn、Leu-Glu、Leu-Val、及びIle-Valよりなる群から選択される少なくとも1種のジペプチドである、[12]に記載の筋肉合成促進方法、
[14]ホエイタンパク質加水分解物が、ホエイタンパク質をタンパク質分解酵素により加水分解することにより得られる、[12]又は[13]に記載の筋肉合成促進方法、
[15]タンパク質分解酵素がバシラス属由来タンパク質分解酵素、アスペルギルス属由来タンパク質分解酵素、トリプシン、ペプシン、フレーバザイムより選ばれる1種又は2種以上である、[14]に記載の筋肉合成促進方法、
[16]タンパク質分解酵素による加水分解を、20~80℃で2~48時間で行う、[14]又は[15]に記載の筋肉合成促進方法、
[17]ホエイタンパク質加水分解物を一食摂取量として総タンパク質含量換算で1~40gで対象に摂取させる、[12]~[15]のいずれか1に記載の筋肉合成促進方法、
[18]対象が運動する対象である、[12]~[17]のいずれか1に記載の筋肉合成促進方法、
[19]運動が筋肉トレーニングである、[18]に記載の筋肉合成促進方法。
[20]筋肉合成促進剤の製造のための、ホエイタンパク質加水分解物の使用。
[21]筋肉合成促進剤としての、ホエイタンパク質加水分解物の使用。
[22]筋肉合成促進のための、ホエイタンパク質加水分解物。 That is, the present invention is as follows.
[1] A muscle synthesis promoter comprising whey protein hydrolyzate as an active ingredient,
[2] The whey protein hydrolyzate contains a dipeptide, and the dipeptide
(1) Ile-Leu and Leu-Ala, and (2) Ala-Leu, Val-Leu, Lys-Ile, Leu-Leu, Ile-Asn, Leu-Glu, Leu-Val, and Ile-Val The muscle synthesis promoter according to [1], which is at least one dipeptide selected from the group,
[3] The muscle synthesis promoter according to [1] or [2], wherein the whey protein hydrolyzate is obtained by hydrolyzing whey protein with a proteolytic enzyme,
[4] The muscle synthesis promoter according to [3], wherein the proteolytic enzyme is one or more selected from Bacillus genus proteolytic enzyme, Aspergillus genus proteolytic enzyme, trypsin, pepsin, and flavorzyme,
[5] The muscle synthesis promoter according to [3] or [4], wherein hydrolysis with a proteolytic enzyme is performed at 20 to 80 ° C. for 2 to 48 hours,
[6] Any one of [1] to [5], comprising a unit packaging form per serving, wherein the unit packaging form contains 1 to 40 g of whey protein hydrolyzate in terms of the total protein content The muscle synthesis promoter according to 1,
[7] The muscle synthesis promoter according to any one of [1] to [6] for a subject to exercise,
[8] The muscle synthesis promoter according to [7], wherein the exercise is muscle training,
[9] The agent according to any one of [1] to [8], which is a composition for promoting muscle synthesis,
[10] The agent according to any one of [1] to [9], which is a food or drink or a pharmaceutical product.
[11] A method for producing a muscle synthesis promoter, comprising blending an effective amount of a whey protein hydrolyzate.
[12] A method for promoting muscle synthesis, which comprises allowing a subject in need thereof to take an effective amount of a whey protein hydrolyzate,
[13] The whey protein hydrolyzate contains a dipeptide, and the dipeptide
(1) Ile-Leu and Leu-Ala, and (2) Ala-Leu, Val-Leu, Lys-Ile, Leu-Leu, Ile-Asn, Leu-Glu, Leu-Val, and Ile-Val The method for promoting muscle synthesis according to [12], which is at least one dipeptide selected from the group,
[14] The method for promoting muscle synthesis according to [12] or [13], wherein the whey protein hydrolyzate is obtained by hydrolyzing whey protein with a proteolytic enzyme,
[15] The method for promoting muscle synthesis according to [14], wherein the proteolytic enzyme is one or more selected from Bacillus-derived proteolytic enzyme, Aspergillus-derived proteolytic enzyme, trypsin, pepsin, and flavorzyme,
[16] The method for promoting muscle synthesis according to [14] or [15], wherein the hydrolysis with a proteolytic enzyme is performed at 20 to 80 ° C. for 2 to 48 hours,
[17] The method for promoting muscle synthesis according to any one of [12] to [15], wherein the subject is ingested with 1 to 40 g of whey protein hydrolyzate as a meal intake in terms of total protein content,
[18] The method for promoting muscle synthesis according to any one of [12] to [17], wherein the subject is a subject to exercise,
[19] The muscle synthesis promotion method according to [18], wherein the exercise is muscle training.
[20] Use of a whey protein hydrolyzate for production of a muscle synthesis promoter.
[21] Use of a whey protein hydrolyzate as a muscle synthesis promoter.
[22] A whey protein hydrolyzate for promoting muscle synthesis.
なお、本発明のホエイタンパク質加水分解物を得るために、タンパク質加水分解酵素によりホエイタンパク質を加水分解する場合の、反応温度は、使用するタンパク質加水分解酵素の至適温度を鑑み、1段階、又は2段階以上の反応温度を設定することができる。2段階以上の反応温度を設定する場合には、その順番は任意である。 In order to obtain the whey protein hydrolyzate of the present invention, when the whey protein is hydrolyzed by a protein hydrolase, the reaction temperature is preferably 20 to 80 ° C., more preferably 25 to 75 ° C., and still more preferably 30 It is ˜70 ° C., more preferably 35 to 65 ° C., further preferably 40 to 60 ° C., particularly preferably 45 to 55 ° C. A reaction temperature of 20 ° C. or higher is preferable because whey protein can be efficiently hydrolyzed by a proteolytic enzyme. Moreover, when the reaction temperature is 80 ° C. or lower, inactivation due to denaturation of the proteolytic enzyme is suppressed, which is preferable.
In addition, in order to obtain the whey protein hydrolyzate of the present invention, the reaction temperature when hydrolyzing whey protein with a protein hydrolase is one step in view of the optimum temperature of the protein hydrolase used, or Two or more reaction temperatures can be set. When two or more reaction temperatures are set, the order is arbitrary.
なお、本発明のホエイタンパク質加水分解物を得るために、タンパク質加水分解酵素によりホエイタンパク質を加水分解する場合の、反応温度を2段階以上で設定する場合には、前記の反応時間において、任意に2段階以上の反応温度を設定することができる。 In order to obtain the whey protein hydrolyzate of the present invention, when the whey protein is hydrolyzed by a protein hydrolase, the reaction time is preferably 2 to 48 hours, more preferably 2 to 36 hours, still more preferably 2 -24 hours, more preferably 2-18 hours, more preferably 2-14 hours, more preferably 4-12 hours, still more preferably 6-10 hours, particularly preferably 7-9 hours. A reaction time of 2 hours or longer is preferable because much whey protein can be hydrolyzed by a proteolytic enzyme. A reaction time of 48 hours or less is preferable because the whey protein can be efficiently hydrolyzed.
In order to obtain the whey protein hydrolyzate of the present invention, when the reaction temperature is set in two or more stages when hydrolyzing the whey protein with a protein hydrolase, the reaction time is arbitrarily determined in the above reaction time. Two or more reaction temperatures can be set.
なお、本発明のホエイタンパク質加水分解物を得るために、タンパク質加水分解酵素によりホエイタンパク質を加水分解する場合の、反応pHは、使用するタンパク質加水分解酵素の至適pHを鑑み、1段階、又は2段階以上の反応pHを設定することができる。2段階以上の反応pHを設定する場合には、その順番は任意である。 In order to obtain the whey protein hydrolyzate of the present invention, the reaction pH when hydrolyzing the whey protein with a protein hydrolase is preferably near the optimum pH of the protein hydrolase used. For example, when a proteolytic enzyme derived from the genus Bacillus and a proteolytic enzyme derived from the genus Aspergillus are used in combination, it is preferably pH 5-9, more preferably pH 5.5-8.5, still more preferably pH 6-8, and particularly preferably Has a pH of 6.5 to 7.5.
In addition, in order to obtain the whey protein hydrolyzate of the present invention, when the whey protein is hydrolyzed by a protein hydrolase, the reaction pH is one step in view of the optimum pH of the protein hydrolase used, or Two or more stages of reaction pH can be set. When setting reaction pH of two or more steps, the order is arbitrary.
(1)Ile-Leu、及びLeu-Ala、並びに
(2)Ala-Leu、Val-Leu、Lys-Ile、Leu-Leu、Ile-Asn、Leu-Glu、Leu-Val、及びIle-Valよりなる群から選択される少なくとも1種のジペプチド。
すなわち、(1)のIle-Leu、及びLeu-Alaはそれぞれ、本発明のホエイタンパク質加水分解物に含有されていることが特に好ましく、(2)のAla-Leu、Val-Leu、Lys-Ile、Leu-Leu、Ile-Asn、Leu-Glu、Leu-Val、及びIle-Valの中でも最低1種のジペプチドが、本発明のホエイタンパク質加水分解物に含有されていることが特に好ましい。 Among the above-mentioned specific dipeptides, it preferably includes a specific dipeptide that satisfies the following (1) and (2).
(1) Ile-Leu and Leu-Ala, and (2) Ala-Leu, Val-Leu, Lys-Ile, Leu-Leu, Ile-Asn, Leu-Glu, Leu-Val, and Ile-Val At least one dipeptide selected from the group;
That is, it is particularly preferred that (1) Ile-Leu and Leu-Ala are each contained in the whey protein hydrolyzate of the present invention, and (2) Ala-Leu, Val-Leu, Lys-Ile. It is particularly preferable that at least one dipeptide among Leu-Leu, Ile-Asn, Leu-Glu, Leu-Val, and Ile-Val is contained in the whey protein hydrolyzate of the present invention.
ホエイタンパク質50gを水1Lに溶解させた。溶解したホエイタンパク質溶液をpH調整剤でpH7に調整し、バシラス属由来のプロテアーゼ(プロテアーゼMアマノ、アマノエンザイム社)500mg、及びアスペルギルス属由来のプロテアーゼ(プロテアーゼNアマノ、アマノエンザイム社)500mgを組み合わせて溶液に加え、50℃で8時間、ホエイタンパク質を加水分解した。プロテアーゼを加熱失活後、得られた溶液を凍結乾燥により粉末にしたものを実施例1の筋肉合成促進剤とした。 [Example 1]
50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution is adjusted to
実施例1の筋肉合成促進剤の粉末を0.1%トリフルオロ酢酸水溶液に1000倍(体積比)に希釈し、以下の条件によりLC/MSを用い、Ile-Leu、Ile-Trp、Ala-Leu、Val-Leu、Asp-Leu、Lys-Ile、Leu-Leu、Ile-Asn、Leu-Ala、及びLeu-Gluを定量(単位はmg/gタンパク質)した。
なお、LC/MSの条件は以下の通りである。
分析条件
カラム:Develosil ODS-HG-3(15mm×2mm)
移動相:A液:0.05% TFA水溶液
B液:0.05% TFAアセトニトリル溶液
移動相の通液開始から0minに3体積%B液、40min後に20体積%B液(いずれもA液及びB液の合計に対する割合)となるように、グラジエントをかけた。
カラム温度:35℃
流速:0.2mL/min
MS条件
Ionization:API-ES positive
SIM ion:m/z 245
Drying gas:10L/min at 350℃
Nebulizer:25psig
Fragmentor:30V
EM gain:1 [Test Example 1]
The muscle synthesis promoter powder of Example 1 was diluted 1000 times (volume ratio) in a 0.1% trifluoroacetic acid aqueous solution, and LC / MS was used under the following conditions to obtain Ile-Leu, Ile-Trp, Ala- Leu, Val-Leu, Asp-Leu, Lys-Ile, Leu-Leu, Ile-Asn, Leu-Ala, and Leu-Glu were quantified (unit: mg / g protein).
The LC / MS conditions are as follows.
Analysis condition column: Develosil ODS-HG-3 (15 mm × 2 mm)
Mobile phase: Liquid A: 0.05% TFA aqueous solution Liquid B: 0.05%
Column temperature: 35 ° C
Flow rate: 0.2 mL / min
MS condition Ionization: API-ES positive
SIM ion: m / z 245
Drying gas: 10 L / min at 350 ° C.
Nebulizer: 25 psig
Fragmentor: 30V
EM gain: 1
以下の通り、実施例1の筋肉合成促進剤による筋肉合成促進効果を検証した。
(1)ラットの水泳試験
実験動物には、初期体重が130~170gのSD系雄性ラット(日本クレア株式会社より入手した)を用いた。
固形飼料(オリエンタル酵母工業株式会社製)を用いて、ラットを3~5日間予備飼育した後、水泳運動に馴化させるために、ラットを30分間の水泳運動(水温34℃)に供した。馴化水泳運動実施日の翌日から翌々日にかけて16時間絶食させた後、ラットを試験群ごとで体重差が生じないように5つに分けた(各試験群8尾ずつ)。その後、ラットを2時間の水泳運動に供した。水泳運動終了直後に、水(すなわち、0gタンパク質/kg体重)又は筋肉合成促進剤2.5重量%又は10重量%の溶液又はホエイタンパク質2.5重量%又は10重量%の溶液を20mL/kg体重(すなわち、0.5gタンパク質/kg体重又は2.0gタンパク質/kg体重)でラットに経口摂取させた。経口摂取から45分後に、重水素ラベルフェニルアラニン溶液(重水素ラベルフェニルアラニン225mgを生理食塩水10mLに溶解して調製した)を2.0mL/kg体重でラットに尾静脈投与した。尾静脈投与から15分後に(すなわち経口摂取から60分後に)、イソフルラン麻酔下でラットを解剖した。腹部大静脈より全採血した後に、上肢三頭筋を摘出し、生理食塩水で洗浄後、直ちに液体窒素で凍結した。尾静脈投与の時刻及び上肢三頭筋の凍結保存の時刻は正確に記録した。上肢三頭筋は分析時まで-80℃に保存した。 [Test Example 2]
The muscle synthesis promoting effect of the muscle synthesis promoter of Example 1 was verified as follows.
(1) Rat swimming test SD male rats (obtained from CLEA Japan, Inc.) having an initial weight of 130 to 170 g were used as experimental animals.
The rats were preliminarily raised for 3 to 5 days using a solid feed (Oriental Yeast Co., Ltd.), and then subjected to a 30-minute swimming exercise (water temperature: 34 ° C.) in order to acclimate to the swimming exercise. After fasting for 16 hours from the next day to the day after the acclimated swimming exercise, the rats were divided into five groups (8 in each test group) so that there was no difference in body weight between the test groups. Thereafter, the rats were subjected to a 2-hour swimming exercise. Immediately after the end of the swimming exercise, 20 mL / kg of water (ie 0 g protein / kg body weight) or a muscle synthesis promoter 2.5% or 10% by weight solution or whey protein 2.5% or 10% by weight solution Rats were orally ingested at body weight (ie 0.5 g protein / kg body weight or 2.0 g protein / kg body weight). 45 minutes after oral ingestion, a deuterium labeled phenylalanine solution (prepared by dissolving 225 mg of deuterium labeled phenylalanine in 10 mL of physiological saline) was administered to the rat via the tail vein at 2.0 mL / kg body weight. Rats were dissected under isoflurane anesthesia 15 minutes after tail vein administration (ie 60 minutes after oral ingestion). After whole blood was collected from the abdominal vena cava, the triceps muscle of the upper limb was removed, washed with physiological saline, and immediately frozen with liquid nitrogen. The time of tail vein administration and the time of cryopreservation of the upper limb triceps were accurately recorded. The upper limb triceps were stored at −80 ° C. until analysis.
先行文献(A. Kanda et al., Br J Nutr, Sep. 28, 981-987, 2013)の記載に基づいて、摘出、保存した上肢三頭筋についての骨格筋合成速度(FSR)を測定した。具体的には、骨格筋細胞のタンパク質に同化して存在する、あるいは骨格筋細胞中に遊離状態で存在する、フェニルアラニン(Phe)及び重水素ラベルフェニルアラニン(Phe(Ring-D5))量を、LC/MS/MS(Waters社製)を用いてそれぞれ定量した。この測定条件は以下の通りである。すなわち、装置:LC/MS/MS(Waters社製)、カラム:ACQUITY UPLC BEH C18(Waters社製)、移動相:0.05%TFA水/0.05%TFA含有アセトニトリル、注入量 3μL、流速 0.3mL/min、UV 215nmである。 (2) Measurement of skeletal muscle synthesis rate (FSR) The triceps muscle of the extremity extracted and stored based on the description in the previous literature (A. Kanda et al., Br J Nutr, Sep. 28, 981-987, 2013) The skeletal muscle synthesis rate (FSR) for was measured. Specifically, the amounts of phenylalanine (Phe) and deuterium-labeled phenylalanine (Phe (Ring-D5)), which are assimilated to the protein of skeletal muscle cells or are present in the skeletal muscle cells in a free state, are calculated using LC Quantification was performed using / MS / MS (manufactured by Waters). The measurement conditions are as follows. That is, apparatus: LC / MS / MS (manufactured by Waters), column: ACQUITY UPLC BEH C18 (manufactured by Waters), mobile phase: acetonitrile containing 0.05% TFA water / 0.05% TFA,
FSR(%/day)=(Eb×100)/(Ea×t)
Ea:エンリッチメント(骨格筋細胞中に遊離状態で存在)
Eb:エンリッチメント(骨格筋細胞のタンパク質に同化して存在)
t:尾静脈投与から、上肢三頭筋を凍結保存するまでの時間(単位:日)
エンリッチメント=Phe(Ring-D5)/(Phe+Phe(Ring-D5)) Here, the skeletal muscle synthesis rate (FSR) was calculated by the following equation.
FSR (% / day) = (Eb × 100) / (Ea × t)
Ea: enrichment (present in free state in skeletal muscle cells)
Eb: enrichment (present assimilated to skeletal muscle cell protein)
t: Time from administration of tail vein to cryopreservation of triceps surae (unit: days)
Enrichment = Phe (Ring−D5) / (Phe + Phe (Ring−D5))
ホエイタンパク質50gを水1Lに溶解させた。溶解したホエイタンパク質溶液をpH調整剤でpH7に調整し、バシラス属由来のプロテアーゼ(プロテアーゼMアマノ、アマノエンザイム社)500mgを溶液に加え、50℃で8時間、ホエイタンパク質を加水分解した。プロテアーゼを加熱失活後、得られた溶液を凍結乾燥により粉末にしたものを実施例2の筋肉合成促進剤とした。 [Example 2]
50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution was adjusted to
ホエイタンパク質50gを水1Lに溶解させた。溶解したホエイタンパク質溶液をpH調整剤でpH7に調整し、アスペルギルス属由来のプロテアーゼ(プロテアーゼNアマノ、アマノエンザイム社)500mgを溶液に加え、50℃で8時間、ホエイタンパク質を加水分解した。プロテアーゼを加熱失活後、得られた溶液を凍結乾燥により粉末にしたものを実施例3の筋肉合成促進剤とした。 [Example 3]
50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution was adjusted to
ホエイタンパク質50gを水1Lに溶解させた。溶解したホエイタンパク質溶液をpH調整剤でpH7に調整し、トリプシン(ノボ社)500mgを溶液に加え、37℃で4時間反応後、バシラス属由来のプロテアーゼ(プロテアーゼMアマノ、アマノエンザイム社)500mgを溶液に加え、50℃で4時間、ホエイタンパク質を加水分解した。プロテアーゼを加熱失活後、得られた溶液を凍結乾燥により粉末にしたものを実施例4の筋肉合成促進剤とした。 [Example 4]
50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution is adjusted to
ホエイタンパク質50gを水1Lに溶解させた。溶解したホエイタンパク質溶液をpH調整剤でpH7に調整し、トリプシン(ノボ社)500mgを溶液に加え、37℃で4時間反応後、アスペルギルス属由来のプロテアーゼ(プロテアーゼNアマノ、アマノエンザイム社)、50℃で4時間、ホエイタンパク質を加水分解した。プロテアーゼを加熱失活後、得られた溶液を凍結乾燥により粉末にしたものを実施例5の筋肉合成促進剤とした。 [Example 5]
50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution was adjusted to
ホエイタンパク質50gを水1Lに溶解させた。溶解したホエイタンパク質溶液をpH調整剤でpH7に調整し、ペプシン(和光純薬社)500mgを溶液に加え、37℃で4時間反応後、バシラス属由来のプロテアーゼ(プロテアーゼMアマノ、アマノエンザイム社)500mgを溶液に加え、50℃で4時間、ホエイタンパク質を加水分解した。プロテアーゼを加熱失活後、得られた溶液を凍結乾燥により粉末にしたものを実施例6の筋肉合成促進剤とした。 [Example 6]
50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution is adjusted to
ホエイタンパク質50gを水1Lに溶解させた。溶解したホエイタンパク質溶液をpH調整剤でpH7に調整し、ペプシン(和光純薬社)500mgを溶液に加え、37℃で4時間反応後、アスペルギルス属由来のプロテアーゼ(プロテアーゼNアマノ、アマノエンザイム社)、50℃で4時間、ホエイタンパク質を加水分解した。プロテアーゼを加熱失活後、得られた溶液を凍結乾燥により粉末にしたものを実施例7の筋肉合成促進剤とした。 [Example 7]
50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution is adjusted to
ホエイタンパク質50gを水1Lに溶解させた。溶解したホエイタンパク質溶液をpH調整剤でpH7に調整し、フレーバザイム(ノボ社)500mgを溶液に加え、50℃で6時間、ホエイタンパク質を加水分解した。プロテアーゼを加熱失活後、得られた溶液を凍結乾燥により粉末にしたものを実施例8の筋肉合成促進剤とした。 [Example 8]
50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution was adjusted to
ホエイタンパク質50gを水1Lに溶解させた。溶解したホエイタンパク質溶液をpH調整剤でpH7に調整し、アスペルギルス属由来のプロテアーゼ(ウマミザイム、アマノエンザイム社)500mgを溶液に加え、50℃で6時間、ホエイタンパク質を加水分解した。プロテアーゼを加熱失活後、得られた溶液を凍結乾燥により粉末にしたものを実施例9の筋肉合成促進剤とした。 [Example 9]
50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution was adjusted to
ホエイタンパク質50gを水1Lに溶解させた。溶解したホエイタンパク質溶液をpH調整剤でpH7に調整し、アスペルギルス属由来のプロテアーゼ(プロテアーゼAアマノ、アマノエンザイム社)500mgを溶液に加え、50℃で6時間、ホエイタンパク質を加水分解した。プロテアーゼを加熱失活後、得られた溶液を凍結乾燥により粉末にしたものを実施例10の筋肉合成促進剤とした。 [Example 10]
50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution was adjusted to
ホエイタンパク質50gを水1Lに溶解させた。溶解したホエイタンパク質溶液をpH調整剤でpH7に調整し、アスペルギルス属由来のプロテアーゼ(プロテアーゼPアマノ、アマノエンザイム社)500mgを溶液に加え、50℃で6時間、ホエイタンパク質を加水分解した。プロテアーゼを加熱失活後、得られた溶液を凍結乾燥により粉末にしたものを実施例11の筋肉合成促進剤とした。 [Example 11]
50 g of whey protein was dissolved in 1 L of water. The dissolved whey protein solution was adjusted to
Claims (15)
- ホエイタンパク質加水分解物を有効成分とする筋肉合成促進剤。 A muscle synthesis promoter containing whey protein hydrolyzate as an active ingredient.
- ホエイタンパク質加水分解物が、ジペプチドを含み、前記ジペプチドが、
(1)Ile-Leu、及びLeu-Ala、並びに
(2)Ala-Leu、Val-Leu、Lys-Ile、Leu-Leu、Ile-Asn、Leu-Glu、Leu-Val、及びIle-Valよりなる群から選択される少なくとも1種のジペプチドである、請求項1に記載の筋肉合成促進剤。 The whey protein hydrolyzate contains a dipeptide, and the dipeptide
(1) Ile-Leu and Leu-Ala, and (2) Ala-Leu, Val-Leu, Lys-Ile, Leu-Leu, Ile-Asn, Leu-Glu, Leu-Val, and Ile-Val The muscle synthesis promoter according to claim 1, which is at least one dipeptide selected from the group. - ホエイタンパク質加水分解物が、ホエイタンパク質をタンパク質分解酵素により加水分解することにより得られる、請求項1又は請求項2に記載の筋肉合成促進剤。 The muscle synthesis promoter according to claim 1 or 2, wherein the whey protein hydrolyzate is obtained by hydrolyzing whey protein with a proteolytic enzyme.
- タンパク質分解酵素がバシラス属由来タンパク質分解酵素、アスペルギルス属由来タンパク質分解酵素、トリプシン、ペプシン、フレーバザイムより選ばれる1種又は2種以上である、請求項3に記載の筋肉合成促進剤。 The muscle synthesis promoter according to claim 3, wherein the proteolytic enzyme is one or more selected from Bacillus genus proteolytic enzyme, Aspergillus genus proteolytic enzyme, trypsin, pepsin, and flavorzyme.
- タンパク質分解酵素による加水分解が、20~80℃で2~48時間で行われる、請求項3又は請求項4に記載の筋肉合成促進剤。 The muscle synthesis promoter according to claim 3 or 4, wherein hydrolysis with a proteolytic enzyme is performed at 20 to 80 ° C for 2 to 48 hours.
- 一食あたりの単位包装形態からなり、該単位包装形態中に、ホエイタンパク質加水分解物を一食摂取量として総タンパク質含量換算で1~40g含む、請求項1~請求項5のいずれか1項に記載の筋肉合成促進剤。 The unit packaging form according to any one of claims 1 to 5, wherein the unit packaging form contains 1 to 40 g of whey protein hydrolyzate in terms of a single meal intake in terms of total protein content. The muscle synthesis promoter as described.
- 運動する対象のための、請求項1~請求項6のいずれか1項に記載の筋肉合成促進剤。 The muscle synthesis promoter according to any one of claims 1 to 6, for a subject to exercise.
- 運動が筋肉トレーニングである、請求項7に記載の筋肉合成促進剤。 The muscle synthesis promoter according to claim 7, wherein the exercise is muscle training.
- 筋肉合成促進用組成物である、請求項1~請求項8のいずれか1項に記載の剤。 The agent according to any one of claims 1 to 8, which is a composition for promoting muscle synthesis.
- 飲食品または医薬品である、請求項1~請求項9のいずれか1項に記載の剤。 The agent according to any one of claims 1 to 9, which is a food or drink or a pharmaceutical product.
- ホエイタンパク質加水分解物の有効量を配合すること含む、筋肉合成促進剤の製造方法。 A method for producing a muscle synthesis promoter comprising blending an effective amount of a whey protein hydrolyzate.
- ホエイタンパク質加水分解物の有効量を、それを必要とする対象に摂取させることを含む、筋肉合成促進方法。 A method for promoting muscle synthesis, which comprises allowing a subject in need thereof to take an effective amount of a whey protein hydrolyzate.
- 筋肉合成促進剤の製造のための、ホエイタンパク質加水分解物の使用。 Use of whey protein hydrolyzate for production of muscle synthesis promoter.
- 筋肉合成促進剤としての、ホエイタンパク質加水分解物の使用。 Use of whey protein hydrolyzate as a muscle synthesis promoter.
- 筋肉合成促進のための、ホエイタンパク質加水分解物。
A whey protein hydrolyzate to promote muscle synthesis.
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201680041243.7A CN108025032A (en) | 2015-08-10 | 2016-08-08 | Muscle synthesis accelerant |
SG11201710681XA SG11201710681XA (en) | 2015-08-10 | 2016-08-08 | Muscle synthesis-stimulating agent |
JP2017534432A JPWO2017026429A1 (en) | 2015-08-10 | 2016-08-08 | Muscle synthesis promoter |
HK18113381.0A HK1254238A1 (en) | 2015-08-10 | 2018-10-18 | Muscle synthesis promoting agent |
Applications Claiming Priority (2)
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JP2015-158295 | 2015-08-10 | ||
JP2015158295 | 2015-08-10 |
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WO2017026429A1 true WO2017026429A1 (en) | 2017-02-16 |
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PCT/JP2016/073233 WO2017026429A1 (en) | 2015-08-10 | 2016-08-08 | Muscle synthesis promoting agent |
Country Status (5)
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JP (1) | JPWO2017026429A1 (en) |
CN (1) | CN108025032A (en) |
HK (1) | HK1254238A1 (en) |
SG (1) | SG11201710681XA (en) |
WO (1) | WO2017026429A1 (en) |
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WO2018164254A1 (en) * | 2017-03-10 | 2018-09-13 | 株式会社明治 | Composition for promoting increase in bdnf level in body |
WO2018221526A1 (en) * | 2017-06-01 | 2018-12-06 | 株式会社明治 | Nutritional composition |
JP2020176100A (en) * | 2019-04-22 | 2020-10-29 | 株式会社明治 | Compositions for suppressing exercise-induced muscle damage |
WO2021060927A1 (en) * | 2019-09-25 | 2021-04-01 | 매일유업 주식회사 | Composition for alleviating, preventing or treating sarcopenia, containing whey protein hydrolysate as active ingredient |
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2016
- 2016-08-08 SG SG11201710681XA patent/SG11201710681XA/en unknown
- 2016-08-08 WO PCT/JP2016/073233 patent/WO2017026429A1/en active Application Filing
- 2016-08-08 CN CN201680041243.7A patent/CN108025032A/en active Pending
- 2016-08-08 JP JP2017534432A patent/JPWO2017026429A1/en active Pending
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2018
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Cited By (8)
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WO2018164254A1 (en) * | 2017-03-10 | 2018-09-13 | 株式会社明治 | Composition for promoting increase in bdnf level in body |
JP6435079B1 (en) * | 2017-03-10 | 2018-12-05 | 株式会社明治 | Composition for promoting an increase in the amount of BDNF in the body |
CN110381965A (en) * | 2017-03-10 | 2019-10-25 | 株式会社明治 | For promoting the internal increased composition of BDNF amount |
WO2018221526A1 (en) * | 2017-06-01 | 2018-12-06 | 株式会社明治 | Nutritional composition |
JPWO2018221526A1 (en) * | 2017-06-01 | 2020-04-02 | 株式会社明治 | Nutrition composition |
JP7198749B2 (en) | 2017-06-01 | 2023-01-04 | 株式会社明治 | nutritional composition |
JP2020176100A (en) * | 2019-04-22 | 2020-10-29 | 株式会社明治 | Compositions for suppressing exercise-induced muscle damage |
WO2021060927A1 (en) * | 2019-09-25 | 2021-04-01 | 매일유업 주식회사 | Composition for alleviating, preventing or treating sarcopenia, containing whey protein hydrolysate as active ingredient |
Also Published As
Publication number | Publication date |
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CN108025032A (en) | 2018-05-11 |
JPWO2017026429A1 (en) | 2018-05-31 |
SG11201710681XA (en) | 2018-01-30 |
HK1254238A1 (en) | 2019-07-12 |
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