WO2016195304A1 - Composition for improvement of liver function containing natural extract - Google Patents

Abstract

The present invention relates to a composition for the improvement of liver function containing a natural extract and, more specifically, to a composition for the improvement of liver function which exhibits effects of the improvement of a fatty liver, the alleviation of stress, and the alleviation of heavy metal poisoning by containing any two or more of chitooligosaccharide, a post-fermented tea extract, and a milk thistle extract. Using the composition for the improvement of liver function according to the present invention improves a fatty liver, and alleviates the effects of heavy metal poisoning by promoting the detoxification function of a liver and promoting an excellent effect of alleviating stress and fatigue by improving liver functions.

Description

Composition for improving liver function containing natural extract

The present invention relates to a composition for improving liver function containing a natural extract, and more specifically, by containing any two or more of chitooligosaccharide, post-fermented tea extract, and milk thistle extract to improve fatty liver, relieve stress, and alleviate heavy metal poisoning. It relates to a composition for improving liver function.

The liver is the largest organ in the human body, and the main function of the liver is to process various substances introduced or produced in the body and to synthesize and supply important substances. Blood also contains several proteins that play an important role in our bodies, of which about 90% are made in the liver. In addition, the liver acts as a detoxification function and immune organs, and various drugs and harmful substances that enter our body are converted into less harmful substances in the liver and excreted through urine or bile. In addition, the liver has an immune cell called Cooper cells, which eat bacteria and toxins or foreign substances coming from outside the body and break it down and release it out of the body. In addition, the liver has a strong regeneration, and even after 75% ablation, the size and function is restored after 4-6 months.

As such, since the liver performs various important functions, various problems occur when the liver function decreases. In particular, fatty liver, which is easy to develop in modern people who consume excessive alcohol, is one of the representative liver diseases. Most of the fat accumulated in fatty liver is triglyceride, and fatty liver can be divided into alcoholic fatty liver due to heavy drinking and non-alcoholic fatty liver due to obesity, diabetes, hyperlipidemia or drugs. Alcoholic fatty liver is caused by excessive intake of alcohol, which promotes fat synthesis in the liver and does not undergo normal energy metabolism.

Fatty liver is one of the most serious liver diseases, considering that fat goes beyond simply accumulation of fat in the liver, and that 50% of patients found to be alcoholic fatty liver and 30% of patients found to be nonalcoholic fatty liver develop liver cirrhosis. You should see. In particular, alcoholic fatty liver may progress to chronic liver disease. Among patients with alcoholic fatty liver, 10-35% are reported to progress to hepatitis, 8-20% to cirrhosis, and alcoholic fatty liver patients compared to nonalcoholic fatty liver patients. Alcoholic fatty liver is a more serious problem because they have more cirrhosis and are more likely to develop hepatitis.

In addition, modern people cannot avoid accumulating toxicity in the human body due to air pollution, contamination with pesticides or heavy metals, and overdose of antibiotics. Excessive influence In addition, modern people are in an environment where they are easily exposed to stress, and their immune system is reduced and their liver function is prone to deterioration. Therefore, preventing the deterioration of liver function and improving liver function are very important for maintaining the general health of modern people.

Therefore, much effort has been made in developing liver function improving foods or drugs containing natural extracts. Post fermented tea extract is a natural extract that can promote liver function improvement. Green tea is largely divided into non-fermented tea and fermented tea, and fermented tea is classified into weakly fermented tea, semi-fermented tea, and post-fermented tea according to the degree of fermentation. A representative example of the post-fermented tea is ivory tea derived from Boi County, China. Boi tea is a post-fermented tea obtained from a broad-leaved tea tree, which destroys the enzymes of green tea leaves and then deposits green tea leaves to induce the reproduction of microorganisms in the air, causing fermentation to occur again. In Korean Patent Application No. 10-2009-0052982, the effect of reducing the blood alcohol concentration of post-fermented tea obtained by fermentation of microorganisms has been disclosed.

Chito-oligosaccharides have 2 to 10 sugars by enzymatically treating chitosan obtained by deacetylating the shells of shellfish (crabs, shrimp, etc.) and the bones of molluscs (squids, cuttlefish, etc.), deproteinizing, and desalting chitin. It has been found that, like chitosan, LDL-cholesterol in the body is significantly reduced and HDL-cholesterol is significantly increased. In Korean Patent Application No. 10-2009-0116467, it has been disclosed that the use of post-fermented tea extract and chitooligosaccharide in a suitable ratio may improve the hangover by promoting the activity of acetaldehyde dehydrogenase.

In addition, silymarin in milk thistle extract, like other flavonoids, is a potent antioxidant, promotes hepatocyte regeneration by activating protein synthesis in hepatocytes, and removes free radicals to prevent liver damage. It is known to be effective in strengthening liver function. Recently, it has been reported that inhibition of expression of inflammation-related factors by inhibition of the activity of kupper cells, a type of macrophages present in liver tissues, is associated with the protective activity of milk thistle. Korean Patent Application No. 10-2011-0076975 has been disclosed that there is an antioxidant effect when using the silymarin and glutathione together.

However, the effects of improving fatty liver, stress relief, and heavy metal poisoning of post-fermented tea extract, chitooligosaccharide, and milk thistle extract are not known. It has not been disclosed in the prior art that the effects are enhanced by synergy when any two or more of the extracts are mixed.

Accordingly, the present inventors have made efforts to develop a composition containing a natural extract to improve the liver function, in particular, the effect of improving the fatty liver, heavy metal poisoning effect and stress relief effect by improving liver function, post-fermented tea extract, It was found that the composition containing any two or more of chitooligosaccharide and milk thistle extract has an excellent liver function improving effect, and has completed the present invention.

Accordingly, an object of the present invention is a composition for improving liver function containing any two or more natural extracts selected from the group consisting of post-fermented tea extract, chitooligosaccharide, and milk thistle extract as an active ingredient, liver function comprising the composition To provide an improved dietary supplement and pharmaceutical composition, and the use of the composition.

In order to achieve the above object, the present invention provides a composition for improving liver function containing any two or more natural extracts selected from the group consisting of post-fermented tea extract, chitooligosaccharide, and milk thistle extract as an active ingredient.

In addition, the present invention provides a health functional food for improving liver function, including the composition and food-acceptable additives.

In addition, the present invention provides a pharmaceutical composition for improving liver function, including the composition and a pharmaceutically acceptable additive.

The present invention also provides the use of the composition for the manufacture of health functional foods to improve liver function.

The present invention also provides the use of the composition for the preparation of a pharmaceutical composition for improving liver function.

When the composition for improving liver function according to the present invention is used, fatty liver is improved, and heavy metal poisoning is alleviated by promoting liver detoxification function, and stress and fatigue relief effect due to improvement of liver function is excellent.

1 is a graph showing the change in content of triglyceride (TG) accumulated in the liver (mg / dl).

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is well known and commonly used in the art.

In the present specification, "post-fermented tea" refers to tea fermented and aged using specific microorganisms under conditions such as proper moisture and temperature after deactivating enzymes in green tea by applying heat, and using enzymes inside tea leaves. It is distinguished from fermented tea.

The microorganism used in the preparation of the post-fermented tea is an edible non-pathogenic microorganism, and may be Saccharomyces sp . , Bacillus sp . Or Aspergillus sp. It may be a strain derived from enteric meat. The strain derived from soy sauce may be a strain derived from soybean paste, red pepper paste or soy sauce prepared using meju, or a strain derived from soybean paste prepared using soybean. For example, Bacillus subtilis , Bacillus licheniformis , Bacillus megateriums , Bacillus natto , Bacillus citreus , Bacillus citreus One or more strains selected from the group consisting of Bacillus circulans , Bacillus mesentricus and Bacillus pumilus . More preferably, the enteric strains may be Bacillus subtilis, also called Bacillus subtilis . Bacillus subtilis is used in the production of amylases, proteases, amino acids, antibacterial compounds or surfactants. In particular, since it is not toxic in humans, animals, plants and the like, it can be widely used as a food microorganism.

The post-fermented tea used in the present invention may be prepared by the following method, but is not limited thereto. First, cultured strains of enteric intestine are inoculated into fermentation broth. The fermentation broth acts as a source of water and energy for the microorganism, and for example, may be prepared by mixing 2 to 5 wt% of sugar or fructose and the like based on the total weight of the fermentation broth and adding soy flour. Thereafter, in order to facilitate fermentation metabolism of the inoculated strain, the fermentation broth inoculated with the strain may be subjected to a process of culturing in an incubator. Then, the fermentation broth inoculated with green tea leaves and strains is mixed and then fermented. Fermentation broth to mix may be appropriate 10 to 80% by weight based on the weight of green tea leaves. Fermentation can be carried out in a constant temperature fermenter at 20 ~ 70 ℃ 24 hours to 28 days. If the fermentation temperature exceeds 40 ℃, there is an effect that the growth of the remaining bacteria except for enteric strains, for example Bacillus subtilis bacteria is inhibited or killed. However, if the fermentation temperature is too high, the growth of enteric strains may also be inhibited. The fermentation process as described above may be referred to as post-fermentation, and may be further subjected to drying through hot air drying after the fermentation is completed.

In addition, in the present invention, the post-fermented tea may be used in the form of an extract extracted with water or an organic solvent. The organic solvent used in the present invention may be selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate, chloroform and a mixed solvent of these organic solvents and water, for example, 70% or more of ethanol may be used. At this time, the extraction temperature may be 10 ~ 80 ℃, for example, the extraction time may be extracted for 6 to 24 hours, for example. If the extraction temperature and extraction time is out of the extraction efficiency may decrease or change of components may occur.

The chitooligosaccharide used by this invention can use the thing of the molecular weight of a various range. Preferably, it may have a molecular weight of 3,000 or less, more preferably, may have a molecular weight of 700 to 2,000, even more preferably, may have a molecular weight of 700 to 1,000.

In addition, the chitooligosaccharide used in the present invention may be, for example, the ratio of the chitooligosaccharide of 3 to 7 sugars based on the total weight of the chitooligosaccharide may be 30% by weight or more.

The method for producing chitooligosaccharide as described above is not particularly limited. For example, first, the crab or shrimp shell is separated and purified by chitin by crushing, desalting, protein removal, and impurity removal processes, and then deacetylated to produce chitosan. Then, chitooligosaccharides may be prepared from the chitosan by chemical decomposition using an acid such as an inorganic acid or an organic acid or by using an enzyme. It is also possible to use commercially available chitooligosaccharide products.

In the case of preparing chitooligosaccharide through the enzymatic digestion method, the enzyme that can be used is not particularly limited, but for example, cellulase may be used. As such, the method of producing chitooligosaccharide by enzymatically decomposing chitosan is not particularly limited. For example, after adding purified water to chitosan, 2 to 3% of lactic acid is added and stirred at a temperature of 40 to 60 ° C to prepare a chitosan dispersion containing lactic acid having a solid content of 5 to 10%. The pH of the completely dissolved chitosan dispersion liquid is adjusted to 4-6, and cellulase which is a chitosan degrading enzyme is dissolved in purified water and added. Then, after hydrolysis at 40 ~ 60 ℃ for 14 to 20 hours, heat treatment at 80 ℃ 30 minutes to inactivate the enzyme, and then through the filtration drying process can be prepared chitooligosaccharides.

Milk thistle ( Silybum) used in the present invention marianum is an asteraceae, also known as thistle, a long, shiny green plant with milky leafy veins, and a long, thin plant that divides into wavy, spiny root-leaves at the edges. It is mainly found in embankment fences and abandoned lands. Useful parts of the plant are whole herbs, roots, leaves, seeds, and husks, and thistle seeds contain flavonoid compounds known as silymarin. Thistle seed extract contains 70-80% of silymarin, which promotes metabolism of hepatocytes and prevents damage to hepatocytes from toxicity.

In the present invention, the method of extracting milk thistle extract may be used by appropriately selecting a method commonly known in the art to which the present invention pertains, and may be commercialized and used as a commercially available product.

In an embodiment of the present invention, after ingesting a composition containing any two or more of the after-fermented tea extract, chitooligosaccharide, milk thistle extract triglyceride content measurement accumulated in the liver, blood index measurement, liver cadmium content measurement, As a result of the stress index measurement, it was found that the liver function could be effectively improved by confirming the excellent fatty liver improvement effect, the heavy metal poisoning effect, and the stress relief effect. In addition, when ingesting a composition containing a mixture of the three components in a specific ratio, it was found that the synergistic effect is more excellent liver function improvement effect.

Accordingly, the present invention relates to a composition for improving liver function containing any two or more natural extracts selected from the group consisting of after-fermented tea extract, chitooligosaccharide, and milk thistle extract as an active ingredient.

In the present invention, the composition may be characterized by exhibiting a fatty liver improvement effect. In particular, the triglyceride content accumulated in the fatty liver is reduced, and the triglyceride content of the alcoholic fatty liver and the non-alcoholic fatty liver can be reduced, but it is preferable to reduce the triglyceride content of the alcoholic fatty liver.

In addition, in the present invention, the composition may be characterized by exhibiting a stress relief effect and a heavy metal poisoning effect. If liver function is improved, accumulated fatigue is alleviated and the mind and body are stabilized. Thus, the function of sympathetic and parasympathetic nerves is balanced and stress is improved. In addition, the heavy metal poisoning mitigation effect means to inhibit the accumulation of heavy metals in the liver detoxification function, or to increase the emission of heavy metals. The heavy metal may be preferably cadmium, but is not limited thereto.

In addition, in the present invention, when the post-fermented tea extract, chitooligosaccharide, and milk thistle extract are all contained as an active ingredient, the mixing ratio may be 1 to 3: 1 to 3: 1 by weight, preferably 2: 2: 1.

The composition for improving liver function according to the present invention may contain 10 to 90% by weight of any two or more mixtures selected from the group consisting of the post-fermented tea extract, chitooligosaccharide, and milk thistle extract based on the total weight of the composition. The mixture may be prepared in a formulation containing various content ranges during the preparation process. For example, 10 to 60% by weight is suitable for preparing tablets and soft capsules, and 10 to 90% by weight for preparing hard capsules.

In addition, the composition for improving liver function according to the present invention is easily selected by the person skilled in the art in addition to the post-fermented tea extract, chitooligosaccharide, milk thistle extract, etc. It may be prepared as a food or pharmaceutical composition. In particular, it may be formulated in the form of a hepatic protective agent or a fatigue recovery agent, but is not limited thereto.

In another aspect, the present invention, the use of a mixture of any two or more natural extracts selected from the group consisting of post-fermented tea extract, chitooligosaccharide, and milk thistle extract for the production of health functional foods to improve liver function And it relates to the use of a composition for improving liver function containing the mixture as an active ingredient.

In another aspect, the present invention is the use of any two or more natural extracts selected from the group consisting of post-fermented tea extract, chitooligosaccharide, and milk thistle extract for the production of a pharmaceutical composition for improving liver function and the above It relates to the use of the composition for improving liver function containing the mixture as an active ingredient.

Hereinafter, the present invention will be described in more detail with reference to Examples and Test Examples. These examples and test examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

[ Reference Example  1] Preparation of Post Fermented Tea Extract

<Production of Bacillus Post-fermented Tea>

A strain with an active medium primarily in a centrifuge to recover at 30 ℃ using the vibration incubator for 72 hours culture of the strain Bacillus subtilis (Bacillus Subtilis) it was isolated. The strain was washed three times using 1.0% physiological saline, and then fermented broth was supplied for proper metabolism of microorganisms. The fermentation broth is prepared by mixing 2.5% by weight of sugar with respect to the total weight of the fermentation broth. Then, autoclave sterilization for 15 minutes at a temperature of 27 psi (pounds per square inch), 120 ℃. After sterilization was completed, the mixture was cooled to 25 ° C at room temperature. In order to facilitate the fermentation metabolism of the damaged strain in the washing process, the process was stabilized in the fermentation broth. Specifically, the strain washed three times with saline was mixed with 300 ml of the fermentation broth before adding soybean powder, and stabilized while incubated in the incubator for 24 hours.

Within a sterile reaction tank, the green tea composition prepared by each small packaging units, the fermentation broth and mixed with the Bacillus subtilis Suga The inoculated fermentation broth, the fermentation was adjusted to the number of the strain so that the ^{10 3 ~ 10 8 CFU / ml} . The ratio of fermented broth to the weight of green tea leaves was mixed to be 30-60 wt%. In addition, even after mixing the fermentation broth solution so that the strain is not damaged due to the rapid temperature increase, the tea leaves were continuously stirred to prevent the temperature inside the tea from rising rapidly. After 20 minutes, the reaction was completed and the temperature drop of the green tea fermentation broth mixture blocked the inlet to prevent the inflow of outside air and went through a fermentation process. The fermentation step was carried out in a constant temperature fermenter at 60 ° C. for 7 days. After the fermentation process was completed, the fermented tea was manufactured by hot-air drying at 80-120 degreeC for 5 hours.

After fermentation tea prepared by the above method, pathogenic microorganisms were not detected, and a product with a specification range of 10 ² CFU / g or less in a total microbial account in the final product was obtained.

<Preparation of post-fermented tea extract>

1 kg of the above-fermented tea prepared above was immersed in 5 L of 70% ethanol solution, refluxed at 80 ° C. for 3 hours, and then extracted at room temperature for 12 hours. The extract was filtered, concentrated under reduced pressure, and freeze-dried to prepare a post-fermented tea extract in powder form. Yield was 15-20%, and the prepared powder was stored at 4 ° C. until use.

[ Reference Example  2] Quitooligosaccharides  Produce

After adding purified water to chitosan, lactic acid was added 2 to 3%, stirred at a temperature of 40 to 60 ℃, to prepare a chitosan dispersion containing lactic acid of 5 to 10% solids. The pH of the completely dissolved chitosan dispersion liquid was adjusted to 4-6, and the cellulase which is a chitosan decomposing enzyme was melt | dissolved in purified water, and it injected | threw-in. Then, after 16 hours of hydrolysis at 50 ℃, heat treatment at 80 ℃ 30 minutes to inactivate the enzyme, and then filtered through the drying process to prepare the oligosaccharides.

[ Reference Example  3] Milk Thistle  Preparation of Extract

6 kg of alcohol 6 times in weight ratio was added to 1 kg of dried milk thistle, and refluxed three times, followed by immersion at 45 ° C. for 3 days. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure and purified to obtain a milk thistle extract.

[ Example  1-5 and Comparative example  Preparation of 1-4]

After fermented tea extract prepared by the method of Reference Example 1, chitooligosaccharide prepared by the method of Reference Example 2, milk thistle extract prepared by the method of Reference Example 3 at least two selected by mixing 2: 2: 1 While maintaining the ratio, Example 1-5 mixed so that the concentration of the active ingredient is 258 mg / kg (mg per kg or mpk; treatment capacity per animal unit weight), Comparative Example 1, containing no active ingredient at all Comparative Example 2-4 containing three active ingredients alone at 258 mg / kg was prepared as shown in Table 1 below.

	Example 1	Example 2	Example 3	Example 4	Example 5	Comparative Example 1	Comparative Example 2	Comparative Example 3	Comparative Example 4
Post Fermented Tea Extract (mg / kg)	100	129	172	-	103.2	-	258	-	-
Chitooligosaccharide (mg / kg)	106	129	-	172	103.2	-	-	258	-
Milk Thistle Extract (mg / kg)	52	-	86	86	51.6	-	-	-	258
Total active ingredient (mg / kg)	258	258	258	258	258	0	258	258	258

[ Test Example  1] Fatty liver improvement effect test

Animal experiment was conducted to see if the composition of the present invention has an alcoholic fatty liver improvement effect. First, 80 male rats (Spraque Dawley) of 5 weeks of age were purchased and adjusted to (Samtako Korea Co.) general solid feed for a week, and then divided into a negative control group and a non-alcoholic group. In addition, the alcohol-administered group was classified into an active ingredient-free group (Comparative Example 1), a single active ingredient-containing group (Comparative Example 2-4), and a test group (Example 1-5) according to the composition of the experimental diet. Ten diets each were fed for two weeks. Then, blood samples were collected to analyze blood indices (Table 2), and liver tissue was extracted and then the change in the amount of triglyceride (TG) accumulated in the liver was measured (FIG. 1). At this time, the unit of triglyceride (TG) content was mg / dl (weight of triglyceride contained in the blood 1 deciliter).

As a result, as shown in Figure 1, after the administration of each of Examples 1 to 5 containing the extract of the present invention after the administration of alcohol, the liver TG content was reduced to the level of the negative control group not administered alcohol Could know. In addition, as shown in Table 2, when Examples 1 to 5 were fed to the experimental diet, respectively, it was found that the blood indices related to liver function were all lower than the active ingredient-added group.

	GOT (IU / L)	GPT (IU / L)	BUN (mg / dl)	Creatine (mg / dl)
Negative Control	91.8 ± 17.1	29.4 ± 3.5	12.4 ± 0.8	2.5 ± 0.8
Comparative Example 1 (No additive group)	120.1 ± 11.4	60.3 ± 15.1	17.1 ± 2.5	3.1 ± 1.1
Comparative Example 2	106.7 ± 8.8	55.6 ± 2.1	14.3 ± 2.1	2.3 ± 1.5
Comparative Example 3	108.7 ± 11.8	54.6 ± 9.1	15.3 ± 4.1	2.9 ± 0.5
Comparative Example 4	104.4 ± 3.8	50.1 ± 9.1	14.3 ± 4.1	2.2 ± 0.5
Example 1	94.1 ± 15.2	48.6 ± 5.1	13.8 ± 1.5	2.2 ± 0.4
Example 2	97.1 ± 3.2	51.6 ± 2.1	14.8 ± 1.0	2.2 ± 0.7
Example 3	96.1 ± 7.0	50.6 ± 5.0	15.1 ± 2.5	2.4 ± 2.4
Example 4	99.1 ± 15.1	49.6 ± 3.1	14.8 ± 1.1	2.0 ± 0.4
Example 5	93.1 ± 6.3	49.6 ± 3.2	13.9 ± 0.5	2.3 ± 0.4

In particular, when Example 1 and Example 5 were mixed with all three components, the TG content and blood index accumulated in the liver were much lower than those when Examples 2 to 4 were mixed. In the case of using all of the mixture, post-fermented tea extract, chitooligosaccharide, and milk thistle extract, it was found that the synergistic effect was very excellent in the improvement of fatty liver.

[ Test Example  2] heavy metal poisoning effect test

Animal experiments were conducted to find out whether the composition of the present invention promotes detoxification of the liver and has an effect of relieving heavy metal poisoning. 80 male white rats (Spraque Dawley) of 5 weeks of age were purchased and adapted to (Samtako Korea Co.) general solid feed for a week, and then divided into a negative control group without cadmium and a group receiving cadmium. The cadmium-administered group was further classified into an active ingredient-free group (Comparative Example 1), a single active ingredient-containing group (Comparative Example 2-4), and a test group (Example 1-5), respectively, according to the composition of the experimental diet. The diet was divided into 10 animals for 4 weeks. After 4 weeks, the liver tissue was removed and the cadmium concentration was measured.

As a result, as shown in Table 3, when Examples 1 to 5 were fed to the experimental diet, respectively, the cadmium content accumulated in the liver was significantly reduced compared to the active ingredient-free group. In particular, when Example 1 and Example 5 where all three components were mixed were supplied, the concentration of heavy metals accumulated in the liver was much lower than that when Examples 2 to 4 where only two components were mixed, respectively. When the tea extract, chitooligosaccharide, and milk thistle extract were mixed and used, the synergistic effect of each active ingredient inhibited the accumulation of heavy metals in the liver and the heavy metal poisoning effect of releasing heavy metals was very excellent.

	Liver (ppm)
Negative Control	0.5 ± 0.02
Comparative Example 1 (No additive group)	30.8 ± 4.8
Comparative Example 2	25.3 ± 1.2
Comparative Example 3	26.3 ± 1.2
Comparative Example 4	27.1 ± 1.2
Example 1	18.5 ± 1.9
Example 2	25.3 ± 0.2
Example 3	22.3 ± 1.1
Example 4	21.3 ± 1.8
Example 5	19.9 ± 2.2

[ Example  6-10 and Comparative example  5, manufacturing]

After fermented tea extract prepared by the method of Reference Example 1, chitooligosaccharide prepared by the method of Reference Example 2, milk thistle extract prepared by the method of Reference Example 3 at least two selected by mixing 2: 2: 1 While maintaining the ratio, Example 6-10 mixed with the total weight of the active ingredient to 1,292mg (5 times of Example 1-5) and Comparative Example 5 containing no active ingredient at all as shown in Table 4 below. Prepared.

	Example 6	Example 7	Example 8	Example 9	Example 10	Comparative Example 5
Post Fermented Tea Extract (mg)	500	646	861.33	-	516.8	-
Chitooligosaccharide (mg)	530	646	-	861.33	516.8	-
Milk Thistle Extract (mg)	260	-	430.66	430.66	258.4	-
Total active ingredient (mg)	1,292	1,292	1,292	1,292	1,292	0

[ Test Example  3] stress relief effect test

Human experiment was conducted to see if the composition of the present invention has an effect of relieving stress and fatigue by improving liver function. First, 50 men and women aged 25-45 years were selected to be ingested for 4 weeks at the doses corresponding to the control group (Comparative Example 5) and Example 6-10 through a double-blind experiment. The index was measured and compared.

As a result, as shown in Table 5, when ingesting Examples 6 to 10, respectively, it was confirmed that the physical stress index and the mental stress index was reduced compared to the control group (Comparative Example 5). In particular, in the case of Example 6 and Example 10 in which all of the after-fermented tea extract, chitooligosaccharide, and milk thistle extract were mixed, it was found that the synergistic effect of each active ingredient is very excellent in stress and fatigue relief effect.

	Physical stress index	Mental stress index
Control group (Comparative Example 5)	81 ± 4.8	95 ± 4.8
Example 6	75 ± 2.8	75 ± 3.8
Example 7	79 ± 0.9	78 ± 1.1
Example 8	80 ± 1.1	84 ± 1.9
Example 9	80 ± 1.9	81 ± 1.3
Example 10	76 ± 1.8	77 ± 1.8

The specific parts of the present invention have been described in detail, and it is apparent to those skilled in the art that such specific descriptions are merely preferred embodiments, and thus the scope of the present invention is not limited thereto. something to do. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

[ Formulation example  1] Health food

Post Fermented Tea Extract ............... 500 mg

Quitooligosaccharides .................. 500 mg

Milk Thistle Extract ... 250 mg

Vitamin mixtures

Vitamin A Acetate ......................... 70 μg

Vitamin E ... 1.0 mg

Vitamin B1 ......................................... 0.13 Mg

Vitamin B2 ......................................... 0.15 mg

Vitamin B6 ... 0.5 mg

Vitamin B12 ......................................... 0.2 Μg

Vitamin C ... ..10 mg

Biotin ... ... 10 μg

Nicotinamide ... 1.7 mg

Folic Acid ... ..... 50 ㎍

Calcium Pantothenate ......................................... 0.5 mg

Mineral mixture

Ferrous Sulfate ............. .1.75 mg

Zinc Oxide ... 0.82 mg

Magnesium Carbonate ......................................... 25.3 mg

Potassium monophosphate ........................ .15 mg

Dibasic calcium phosphate ..... .55 mg

Potassium Citrate ... 90 mg

Calcium Carbonate ... .100 mg

Magnesium Chloride ......................................... 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a component suitable for a health food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. The granules may be prepared and used for preparing a health food composition according to a conventional method.

[ Formulation example  2] health drink

Post Fermented Tea Extract ............... 500 mg

Quitooligosaccharides .................. 500 mg

Milk Thistle Extract ... 250 mg

Citric Acid ... .1000 mg

oligosaccharide................................................. .100 g

Plum concentrate ..................... ..2 g

Taurine ... ..... 1 g

Purified water is added to the whole ........... 900 ㎖

After mixing the above components according to the conventional healthy beverage production method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and refrigerated and stored in the health of the present invention Used to prepare beverage compositions.

Although the composition ratio is a composition that is relatively suitable for the preferred beverage in the preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and use purpose.

[ Formulation example  3] Soft capsule

Post-fermented tea extract 500mg, chitooligosaccharide 500mg, milk thistle extract 250mg, soybean extract 50mg, soybean oil 180mg, red ginseng extract 50mg, palm oil 2mg, palm hardened oil 8mg, lead 4mg and lecithin 6mg according to the usual method and 400 per capsule A soft capsule was prepared by filling mg.

[ Formulation example  4] tablets

After fermented tea extract 500mg, chitooligosaccharide 500mg, milk thistle extract 250mg, soybean extract 50mg, glucose 100mg, red ginseng extract 50mg, starch 96mg and magnesium stearate 4mg were added and 30% ethanol was added to form granules, 60 It was dried at 占 폚 and compressed into tablets using a tablet press.

[ Formulation example  5] granules

After fermented tea extract 500mg, chitooligosaccharide 500mg, milk thistle extract 250mg, soybean extract 50mg, glucose 100mg, red ginseng extract 50mg and starch 600mg were mixed, 30% ethanol was added to form a granule, dried at 60 ℃ granules Formed and then filled into fabrics. The final weight of the content was 1 g.

Although the food and pharmaceutical composition formulation examples of the composition for improving liver function according to the present invention have been described in more detail, the food and pharmaceutical compositions are applicable in various formulations, which are not specifically intended to limit the present invention. To explain.

Claims (10)

1. After fermented tea extract, chitooligosaccharide, and milk thistle extract composition for improving liver function containing any two or more natural extracts selected from the group consisting of.
2. The composition for improving liver function according to claim 1, wherein the composition shows a fatty liver improving effect.
3. The composition for improving liver function according to claim 1, wherein the composition exhibits a stress relieving effect.
4. The composition for improving liver function according to claim 1, wherein the composition exhibits a heavy metal poisoning alleviation effect.
5. According to claim 1, wherein the post-fermented tea extract, chitooligosaccharide, and milk thistle extract is all contained as an active ingredient, the mixing ratio is 1-3 by weight ratio, liver function improvement, characterized in that Composition.
6. The composition for improving liver function according to claim 5, wherein the mixing ratio is 2: 2: 1 by weight.
7. A health functional food for improving liver function, comprising the composition of any one of claims 1 to 6 and a food-acceptable additive.
8. A pharmaceutical composition for improving liver function, comprising the composition of any one of claims 1 to 6 and a pharmaceutically acceptable additive.
9. Use of the composition of any one of claims 1 to 6 for the manufacture of a dietary supplement for improving liver function.
10. Use of the composition of any one of claims 1 to 6 for the preparation of a pharmaceutical composition for improving liver function.

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