KR19990026532A - Prevention and improvement agent of liver function disorder caused by chitooligosaccharide - Google Patents

Abstract

The present invention isolates and purifies chitin and chitosan from chitin, which is known as a functional new material of food, and decomposes chitin and chitosan by enzymatic or chemical methods from chitin and chitosan as N-acetyl-D-glucosamine or D-glucosamine. The present invention relates to an agent for preventing and improving liver function disorders in powders, tablets, and liquids containing a specific chitooligosaccharide obtained by preparing a chitooligosaccharide having a degree of polymerization of 2 or more, as a mixture of chitooligosaccharides, or through a separate purification process.

Description

Prevention and improvement agent of liver function disorder caused by chitooligosaccharide

1 is a chemical structure of chitin, according to the present invention,

2 is a process for separating and purifying chitin in accordance with the present invention;

3 is a manufacturing process diagram of the chitooligosaccharide according to the present invention,

Figure 4 is a micrograph of the liver tissue according to the present invention.

[Field of Invention]

The present invention relates to a liver function protection improving agent capable of preventing and treating various liver diseases that may occur due to a decrease in liver function by protecting the liver from factors causing various liver diseases and improving impaired liver function.

[Background of invention]

Soy is the organ that plays the most important role in the metabolism of all animals as well as humans. It has the function of helping to smooth metabolism by controlling nutrients necessary to maintain health and detoxifying metabolites such as free radicals.

The onset of hepatic disease due to the improvement of living standards and the generalization of instant eating has already reached a serious level, and the treatment of various liver diseases is also overflowing. In particular, increased consumption of alcoholic products, which are known to cause serious damage to liver function, and abuse of various drugs and nutrients are known to be a major cause of decreased liver function.

For example, alcohol intake causes various changes in metabolic pathways and pathological changes in the liver and other tissues. Continuous and excessive alcohol consumption causes fat accumulation in liver cells and is characterized by alcoholic necrosis and inflammation. It causes hepatitis. This leads to cirrhosis and eventually leads to death.

Lipid peroxidation, which is considered as one of the basic causes of cell damage and necrosis, is increased due to oxygen free radical or hydrogen peroxide, which occurs naturally during aerobic metabolism in mammalian cells. It is said that the reaction is further accelerated when alcohol is administered.

In order to prevent cellular damage by peroxides, there are protective systems such as glutathione in vivo.

Conventional hepatoprotectants or ameliorators are specific drugs that focus on a treatment system that removes fat accumulated in the liver or thaws damaged hepatocytes and nourishes the hepatocytes. The diet should be combined to restore liver function. Treatment for damage to other body organs due to liver failure requires separate therapeutic drugs or systems.

Recently, as research on various mechanisms causing liver disease progresses, functional foods and drugs that can improve liver function by activating a protective system such as glutathione present in living cells and treat various diseases accompanied by liver function deterioration at the same time Although research is being actively conducted, such effectiveness is not being achieved.

[Purpose of invention]

The present invention is to solve the problems of the conventional liver function protector or improver described above to normalize the damaged metabolism by using functional foods and to activate the self-protection system of the living liver damage that can be caused by alcohol or various drugs It aims to develop functional foods that can prevent and maintain healthy cells by activating damaged liver cells.

[Summary of invention]

In order to achieve the above object, chitin, known as a functional new material of food, is separated and purified into chitin and chitosan, and chitooligosaccharide is prepared from chitin and chitosan as a degradation product of chitin and chitosan by enzymatic or chemical methods. It is characterized by making powders, tablets and liquid liver function protection and improver to contain.

[Description of Specific Embodiment of the Invention]

Hereinafter, embodiments of the present invention will be described in detail.

Chitin is a polysaccharide composed of N-acetyl-D-glucosamine or D-glucosamine as shown in FIG. 1 and is a major component constituting the cell wall and shell skeleton of shellfish, insects, shellfish, and fungi, and is an annual component in the biological system. It is said to be abundant resources on earth after the fiber that produces 100 billion tons.

The safety of chitin has already been proved along with its degradation product, chitooligosaccharide, and it has medical anticancer activity, lowering blood cholesterol level, suppressing hypertension, controlling blood sugar, and immune activating effect. It is also effective in many fields.

Chitooligosaccharides are oligosaccharides that decompose chitin or chitosan by two to ten degrees of polymerization of acetylglucosamine or glucosamine by chemical or enzymatic methods. Unlike chitin and chitosan, which are polymers, they are absorbed into the body and show various functionalities. In particular, the results of animal experiments have been found to be excellent in the effect of protecting and improving the liver function has come to the present invention.

The liver function protective improvement agent of this invention consists of the manufacturing process of a chitooligosaccharide, and the preparation process of a chitooligosaccharide containing agent.

Chitooligosaccharide manufacturing process is a chitin, chitosan isolated and purified as shown in Figure 2, chitin, chitosan or chitosan degrading enzyme of microbial origin from chitin, chitosan isolated and purified as shown in FIG. Decomposition using to make insoluble chitin, chitosan insoluble in distilled water to make a soluble chitooligosaccharide.

The preparation of chitooligosaccharide-containing preparations can be prepared as a functional food or a medicine by combining the chitooligosaccharide complex with 0.01% or more, and combining the additional ingredients in consideration of the palatability and nutritional properties of the formulation.

In the above-described preparation of chitooligosaccharides, chitin is prepared in the state of 5% to 10% colloidal aqueous solution, and the enzyme is reacted after addition of the enzyme at 37 ° C. Heat treatment and filter for inactivation. Chitosan is dissolved in 1% to 2% of acetic acid, lactic acid, citric acid, malic acid, hydrochloric acid, ascorbic acid, etc. to 5% to 10%, or made into a colloidal aqueous solution to decompose chitosan by adding an enzyme at 37 ° C. Made by heat treatment and filtration.

EXAMPLE

1) Preparation of water-soluble low molecular weight and oligosaccharides by chemical treatment of chitin and chitosan.

Chitin and chitosan were hydrolyzed with hydrochloric acid according to the acid decomposition method to remove insoluble matters after purification, and the water-soluble low-molecular weight and oligosaccharides were neutralized.

2) Preparation of water-soluble low molecules and oligosaccharides from chitin by degrading enzymes.

The purified chitin was pulverized to a suspension by a pulverizer, and decomposed for at least 24 hours at 37 ° C.-50 ° C. using purified degrading enzymes to obtain water-soluble low molecules and oligosaccharides, and the insolubles were filtered out. Remove

3) Preparation of water-soluble low molecules and oligosaccharides from chitosan by degrading enzymes.

One or two or more organic acids such as acetic acid, lactic acid, citric acid, ascorbic acid and malic acid are added to the purified chitosan to make a suspension, and the mixture is stirred and dissolved. As shown in FIG. Insoluble matters are removed after filtration of the water-soluble low-molecules and oligosaccharides produced by decomposition at 37 ° C.-50 ° C. for at least 24 hours.

4) Protection and improvement of liver function

In order to examine the effect of chitooligosaccharide on the liver, experimental mice having a body weight of 20 to 30 g at the start of the experiment were placed in each group and used as experimental animals.

The purpose of this study was to observe the cytological changes of mouse liver tissues following the long-term administration of alcohol. The feed was freely supplied with commercial experimental animal feed, divided into three groups, normal drinking water in group A, and ethanol concentration in group B by 10%. Drinking water was prepared so that the C group was tested by freely supplying drinking water dissolved in the ethanol 10% concentration of drinking water so that the chitooligosaccharide concentration of 0.25%.

In alcohol, the ethanol concentration was gradually increased at 1% intervals for the adaptation of the mouse, and histological examination was performed after dissection for 6 weeks (42 days) at 10% concentration.

According to the biopsy results, to recover the liver function of group B, the experimental mouse of group B was dissolved in chitooligosaccharides in 0.25% of normal drinking water and fed freely for 4 weeks (28 days), and then dissected. Classified.

As a result, in group C, there was no significant difference in growth status, activity, feed intake, etc. in the visual observation compared with group A, and there was no obstacle by alcohol. However, the C group showed brown color difference. In the examination of liver tissues, as shown in photographs A and C, no damage sites such as fatty liver phenomenon, inflammation, or necrotic cells appeared, and the health status of liver was similar to that of group A.

In group B, there was a severe decrease in growth status, activity, feed intake, and diarrhea. Liver biopsy results showed typical fatty liver phenomena and inflammation, cell necrosis and cell contraction, as shown in Photo B.

In group D, which is an experiment for restoring liver function of group B, the alcohol supply was stopped and chitooligosaccharide was supplied. As a result, visual observation showed that activity and feed intake were the same as group A. Similar. As a result of liver biopsy after 4 weeks, inflammation and necrotic tissues were markedly reduced and fatty liver phenomenon disappeared as shown in picture D.

[Effects of the Invention]

First, chitooligosaccharides are functional foods, unlike conventional liver function improvers, which are easy to consume and have no side effects by high intake or long term intake.

Second, the improvement of the reduced liver function as well as the suppression and prevention of liver function deterioration by alcohol or various drugs is excellent.

Third, even when mixed with various drugs, the effect of the chitooligosaccharide is not reduced, and the effect of increasing the effect of the mixed drug and the side effect of the drug can be additionally obtained.

Fourth, since the chitooligosaccharide has a bacteriostatic effect, there is no fear of lowering intestinal function, loss of appetite, or indigestion, which are problems of oral medications.

Claims (2)

Functional foods, pharmaceuticals, and mixtures thereof for the purpose of preventing and improving liver function disorders, comprising chitin and oligosaccharides that are degradation products of chitosan. The method of claim 1, wherein a mixture of water-soluble chitooligosaccharides having a degree of polymerization of two or more sugars obtained by preparing chitooligosaccharides from chitin and chitosan as an enzymatic or chemical method is separated and purified into a single oligosaccharide having a specific degree of polymerization to prevent liver function disorders and It includes the manufacture or addition of a functional food or medicament for improvement.