WO2016175618A8 - Method for converting mesenchymal stem cells into endothelial cells by using specific transcription factors - Google Patents

Method for converting mesenchymal stem cells into endothelial cells by using specific transcription factors Download PDF

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Publication number
WO2016175618A8
WO2016175618A8 PCT/KR2016/004557 KR2016004557W WO2016175618A8 WO 2016175618 A8 WO2016175618 A8 WO 2016175618A8 KR 2016004557 W KR2016004557 W KR 2016004557W WO 2016175618 A8 WO2016175618 A8 WO 2016175618A8
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WO
WIPO (PCT)
Prior art keywords
stem cells
mesenchymal stem
transcription factors
cells
endothelial cells
Prior art date
Application number
PCT/KR2016/004557
Other languages
French (fr)
Korean (ko)
Other versions
WO2016175618A1 (en
Inventor
조영애
Original Assignee
가톨릭대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 가톨릭대학교 산학협력단 filed Critical 가톨릭대학교 산학협력단
Priority to US15/570,619 priority Critical patent/US10428307B2/en
Priority claimed from KR1020160053144A external-priority patent/KR101789417B1/en
Publication of WO2016175618A1 publication Critical patent/WO2016175618A1/en
Publication of WO2016175618A8 publication Critical patent/WO2016175618A8/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/44—Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/867—Retroviral vectors

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Vascular Medicine (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Plant Pathology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)

Abstract

The present invention relates to a method for converting mesenchymal stem cells into endothelial cells by using specific transcription factors and, more specifically, a method for converting mesenchymal stem cells into endothelial cells by using Oct4, Nanog, Tal1, and LMO2, which are specific transcription factors. According to the present invention, the method for converting adult cells or mesenchymal stem cells, which are adult stem cells, into endothelial cells was developed by selecting two types of genes, which are less directly related to cancer induction, among cell reprogramming factors and two types of transcription factors, which are not expressed or expressed at a low level in mesenchymal stem cells, among transcription factors related to vascular development, and combining all four. The method can be applied in the production of endothelial cells for forming regenerative tissue in tissue engineering and ischemic disease therapy.
PCT/KR2016/004557 2015-04-29 2016-04-29 Method for converting mesenchymal stem cells into endothelial cells by using specific transcription factors WO2016175618A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US15/570,619 US10428307B2 (en) 2015-04-29 2016-04-29 Method for converting mesenchymal stem cells into endothelial cells by using specific transcription factors

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2015-0060259 2015-04-29
KR20150060259 2015-04-29
KR1020160053144A KR101789417B1 (en) 2015-04-29 2016-04-29 Direct conversion method of mesenchymal stromal stem cells to endothelial cells by defined transcription factors
KR10-2016-0053144 2016-04-29

Publications (2)

Publication Number Publication Date
WO2016175618A1 WO2016175618A1 (en) 2016-11-03
WO2016175618A8 true WO2016175618A8 (en) 2017-11-30

Family

ID=57198874

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2016/004557 WO2016175618A1 (en) 2015-04-29 2016-04-29 Method for converting mesenchymal stem cells into endothelial cells by using specific transcription factors

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WO (1) WO2016175618A1 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1546656A (en) * 2003-12-16 2004-11-17 滨 冯 Method for adult mesenchymal stem cells in vitro directional induction and differentiation to endothelial cell of blood vessel
JP5897002B2 (en) * 2010-07-07 2016-04-13 セルラー ダイナミクス インターナショナル， インコーポレイテッド Endothelial cell production by programming
CN102776150A (en) * 2011-05-13 2012-11-14 上海交通大学医学院附属第九人民医院 Method for inducing umbilical cord mesenchymal stem cells to differentiate into endothelial cells

Also Published As

Publication number Publication date
WO2016175618A1 (en) 2016-11-03

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