WO2016168871A2 - Procédé servant à l'obtention, en particulier à la récolte, d'algues et de microorganismes - Google Patents

Procédé servant à l'obtention, en particulier à la récolte, d'algues et de microorganismes Download PDF

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Publication number
WO2016168871A2
WO2016168871A2 PCT/AT2016/000039 AT2016000039W WO2016168871A2 WO 2016168871 A2 WO2016168871 A2 WO 2016168871A2 AT 2016000039 W AT2016000039 W AT 2016000039W WO 2016168871 A2 WO2016168871 A2 WO 2016168871A2
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WO
WIPO (PCT)
Prior art keywords
reaction medium
filter
microorganisms
collecting container
algae
Prior art date
Application number
PCT/AT2016/000039
Other languages
German (de)
English (en)
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WO2016168871A3 (fr
Inventor
Franz Emminger
Original Assignee
Ecoduna Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ecoduna Ag filed Critical Ecoduna Ag
Publication of WO2016168871A2 publication Critical patent/WO2016168871A2/fr
Publication of WO2016168871A3 publication Critical patent/WO2016168871A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/16—Hollow fibers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass

Definitions

  • the invention relates to a method for the production, in particular for harvesting, of algae and / or microorganisms from a reaction medium, for example for harvesting from a microorganism and nutrient solution
  • Microorganisms preferably phototrophic and especially microalgae as sustainable biogenic raw materials for the production of protein, high-quality materials for the pharmaceutical industry, biofuel, biochemicals are not in competition with food production in the field and can close existing and future supply gaps.
  • Industrially produced C02 can be used by their metabolism as well for recovery of valuable material, as well as slurries from fermentation processes or for example
  • Wastewater from aquaculture For the production of high-quality and therefore high-priced products, a number of harvesting methods are already available today, which allow economic operation of algae production as a whole.
  • a number of harvesting methods are already available today, which allow economic operation of algae production as a whole.
  • bioenergy sector requires an overall positive energy balance in the production chain, which is currently practically impossible to achieve.
  • Compressive stress may cause damage to the algae or microorganisms.
  • DE 10 2009 039 554 A describes a method for harvesting algae from an algal suspension in which a concentration of the algae or microorganisms takes place by means of a membrane filter. For filtering, several stages are run through, using different systems. The disadvantage here is that a very high cost of filtering through the use of different systems is required. This also results in high spare parts costs and a high expenditure of training for the personnel, who must be trained for the most different systems.
  • the object of the invention is to provide a method of the type cited above, which on the one hand avoids the above disadvantages and on the other hand, a continuous, efficient and economic growth process
  • reaction medium continuously, especially during a cleaning cycle for the filter system, a
  • Collection container is supplied by a breeding station and
  • the reaction medium more, in particular at least one further filter stage for increasing the concentration of
  • Reaction medium in particular with algae and / or microorganisms by separation of liquid, in particular the nutrient solution from the reaction medium passes.
  • Reaction medium from the breeding station the promotion in the breeding station not is interrupted. If the production in a biosolar system for a photochemical, such as photocatalytic and / or photosynthetic process come to a standstill, so would significant damage to the algae or
  • Microorganisms occur. Thus, it is extremely important that the harvest must take place during the operation of the breeding station. However, since a breeding station preferably consists of many extraction modules, it is necessary to operate the harvesting unit or the harvesting module with the least possible effort and at the same time to increase the yield. This is realized according to the invention for the first time, with a multi-stage, in particular a two-stage harvest unit.
  • the reaction medium is transported from the collecting container into the filter system, to which a high proportion of
  • Liquid, in particular nutrient solution is separated and the emerging from the filter system, highly concentrated, the reaction medium is in turn fed to the collecting container until a defined concentration of the
  • Reaction medium in particular with algae and / or microorganisms, is achieved in the collecting container. This ensures that the concentrate of algae or microorganisms is always concentrated higher.
  • the reaction medium in the collecting container is conveyed into the further filter stage and preferably, a cleaning process for the filter system is started simultaneously.
  • the feed rate in the first stage always corresponds to the flow rate in the breeding station, so that it is continuously in operation and the best possible growth of algae is achieved.
  • a hollow-fiber membrane is used as filter system, preferably in both filter stages, in which in the first filter stage a solids content between 15 l / min and 50 l / min, in particular 30 l / min and in the second filter stage between 0, 3 l / min and 2 l / min, in particular 1, 0 l / min, is excreted.
  • This ensures that the training required for the maintenance work by the same filter stages can be kept low.
  • Another advantage is that this allows experience during operation in one of the two filter stages can be transferred to the other filter stage, so that the best and maximum yield can be achieved.
  • the quality of the algae or microorganisms can be increased or maintained.
  • a pressure preferably a differential pressure, for acting on the filter system
  • a cleaning process in which the Disconnected catchment container from the rest of the pipeline system and at the same time the cleaning process for the filter system is started. This will be a
  • the transfer of the reaction medium takes place from the collecting container of the first filter stage in the
  • Reaction medium is designed for the overfeed period
  • Filter system in particular the hollow fiber membrane, carried out a cleaning purge with air, in particular with compressed air, or a cleaning liquid.
  • Breeding station from which the reaction medium is continuously withdrawn, must not be interrupted. Thus, it is necessary to keep the structure of the filter stages as simple as possible, so that a self-contained automatic cleaning is feasible.
  • the filter system in the cleaning process for the filter system, is separated from the remaining components, in particular from the collecting container.
  • the filter system is separated from the remaining components, in particular from the collecting container.
  • the membrane filtration system designed as a hollow fiber membrane is formed in a housing corresponding to a three-way valve, wherein the reaction medium laden with algae and / or microorganisms is fed to an inlet and at the other end of the hollow-fiber membrane via an outlet, the concentrated reaction medium to be applied, is applied and at the periphery between the two ends of the hollow fiber membrane via a third outlet, the liquid, in particular the nutrient solution, is applied.
  • Reaction medium which is very high at repeated pass through the algae and only little liquid is present. It is essential in the case of the filter that the algae are preferably transported in the direction of flow, since in the case of a lateral escape they would be pressed against a wall and thus would burst. Strong directional changes should be avoided if possible for the algae.
  • the reactor unit formed of a plurality of reactor elements is guided, wherein the meandering guide of the reaction medium is inclined perpendicularly or at an angle, preferably at least once from bottom to top or against the direction of gravity (ascending branch) and from top to bottom or in the direction of gravity (falling branch), is transported and that at least one, preferably a plurality of reactor units are closed together to form a storage module in which the reaction medium is transported in an endless loop, wherein on a reactor unit of the storage module, preferably Preferably via a removal device, preferably a so-called "Desmodrom", a freely selectable defined proportion of
  • a respective growth module is operated in which the missing portion of the reaction medium is filled to the original amount and / or a removal module for the production of algae and / or
  • Microorganisms is operated.
  • a breeding station is used, in which a very rapid growth of algae or microorganisms is achieved, so that the reaction medium already has a breeding station for high percentage of algae or microorganisms.
  • a breeding station for different types of algae or microorganisms can be used.
  • a further object of the invention is to provide a device with which the economic implementation of the above method is ensured.
  • This object of the invention is achieved by a device for harvesting algae and / or microorganisms from a reaction medium, according to the invention, a collecting container for continuously supplying a
  • Reaction medium with a plant in particular a breeding plant, connected and that for harvesting the reaction medium of algae and / or
  • Microorganisms several, in particular two, filter stages are arranged.
  • the supply from the breeding station does not have to be interrupted, that is, that the breeding station, in particular the
  • Reaction medium in the breeding station is operated continuously and constantly taken out of the extraction modules reaction medium at the end of the breeding station and fed to the harvesting module. It is essential in such systems that the flow in the breeding station is not interrupted, since in this case the algae or microorganisms would sink to the ground and they are destroyed by the building up pressure on the lower algae or microorganisms. Thus, it must be ensured that there is always a flow in the breeding system even during the harvesting process. With such a device a very high reliability is guaranteed.
  • Conveyor such as a pump, in particular a paddle wheel, arranged, which is connected to a filter system, in particular a hollow fiber membrane, and an outlet for the algae and / or microorganisms of
  • Filter system is connected to the collecting container for returning the reaction medium and another outlet is on the filter system for the discharge of liquid, in particular the nutrient solution, for reuse in a breeding plant, arranged.
  • FIG. 1 is a schematic representation of a breeding station with associated harvesting modules or harvesting stations
  • FIG. 2 is a schematic representation of the individual filter stages.
  • a plant in particular a cultivation plant 1 and a harvesting module 2 for harvesting algae and / or microorganisms 3 from a reaction medium 4 is shown.
  • the cultivation plant 1 can be constructed as desired from the prior art, but preferably the plant developed by the applicant is used.
  • the breeding plant 1 consists of a biosol solar system for a photochemical, such as photocatalytic and / or photosynthetic process, in particular for a breeding and production or hydroculturing of, preferably phototrophic, algae or microorganisms 3, wherein the
  • Reaction medium 4 for example, one of a suspension consisting of microorganisms and nutrient solution, meander-shaped in a reactor unit 6 formed from a plurality of reactor elements is performed.
  • the meandering guide of the reaction medium 4 is preferably transported vertically or at an angle at least once from bottom to top or against the direction of gravity (ascending branch) and from top to bottom or in the direction of gravity (falling branch).
  • at least one, preferably a plurality of reactor units 5 are closed together to form a storage module 6, in which the reaction medium 4 is transported in an endless loop.
  • a freely selectable defined proportion of the reaction medium 4 is removed and in a, preferably formed from a plurality of closed reactor units in series 5 line module 7 passed. At the same time, the missing proportion of reaction medium 4 is filled up to the original quantity.
  • the reaction medium 4 is again divided at the last reactor unit 5 and optionally then each supplied to a growth module 8.
  • the reaction medium 4 is fed to a removal module 9 for the recovery of the algae or microorganisms 3.
  • the removal module 9 could also already be arranged behind the line module 7.
  • the illustrated breeding plant 1 is intended to illustrate that the reaction medium 4 in the breeding plant 1 is constantly in motion, wherein the reaction medium 4 preferably via gravity and the
  • Influence of gases as growth-promoting agent is kept in motion.
  • a pressureless delivery of the reaction medium 4 is a
  • the harvesting module 2 are connected to all extraction modules 9 via a connecting line 10.
  • the reaction medium 4 is continuously supplied via the connecting line 10 to the harvesting module 2, whereupon in the second step, the reaction medium 4 more, in particular a further filter stages 11, 12, to increase the
  • the first filter stage 1 1 is dimensioned such that so much reaction medium 4 from the
  • Connecting line 10, in particular the attached breeding station 1 can be included, as long as a cleaning process lasts, so that too during a cleaning process in the first filter stage 1 1 the
  • Reaction medium 4 is supplied.
  • the second filter stage 12 is used for the
  • Microorganisms 3 is returned via a return line 13.
  • Reactor units 5 are used.
  • the individual stages in particular the two filter stages 11 and 12, have the same design.
  • the filter stages 1 1 and 12 differ only with respect to the dimensioning of Nährates- or liquid deposition via a filter system 14, 15.
  • the first filter stage 1 1 namely more liquid is discharged from the reaction medium 4, as in the further filter stage 12th This is the reason that in the second
  • Filter stage 12 is already such a high concentration of algae or microorganisms 3, as shown schematically, with very low liquid content is present, whereas in the first filter stage 1 1 continuously fresh not so highly concentrated reaction medium 4 is supplied from the breeding station 1.
  • Essential in the construction and the design of the harvesting module 2 is that in the first step, the reaction medium 4 continuously, in particular during a cleaning cycle for the filter system 14,15, a collecting container 16 is supplied from the breeding station 1.
  • a collecting container 16 is supplied from the breeding station 1.
  • the second step that goes through
  • Reaction medium 4 more, in particular at least one further filter stage 1 1, 12 for increasing the concentration, in particular of the algae and / or
  • Microorganisms 3 by secretion of liquid, in particular the
  • Microorganisms 3 and the shorter the subsequent (not shown) drying process can fail.
  • the reaction medium 4 is transported from the collecting container 16,17 to the filter system 14, 15, at which a high proportion of liquid, in particular the
  • Reaction medium 4 in particular of algae and / or microorganisms 3, in the collecting container 16,17 is achieved, as shown by arrows 18.
  • Paddle wheel pump with the lowest possible pressure. In particular, it is only used with gravity to avoid damage to the algae and / or
  • the pressure for the filter system 14,15 preferably the differential pressure, for acting on the filter system 14,15, in particular a hollow fiber membrane, 0.3 to 2 bar, preferably 0.7 to 1, 5 bar, in particular about 0.7 to 1, 3 bar.
  • the reaction medium 4 is again via a pipe 21, 22 for
  • the separated from the filter system 14, 15 liquid is at a side exit over the Return line 13 passed back to the breeding station 1, so that this liquid, the nutrient solution, can be used again.
  • the pumps 19, 20 and the filter system 14, 15 can be decoupled from the collecting container 16,17 are in the pipes 21, 22 between
  • a valve 23 is arranged.
  • a liquid, in particular cleaning liquid introduced, which subsequently flows through the pump 19, 20 and the filter system 14,15 and to the other Valves 23 exits again.
  • a cleaning circuit is constructed, which can be carried out independently of the collecting container 16,17.
  • the supply of the reaction medium 4 in the collecting container 16 of the first filter stage 1 1 is carried out at a speed corresponding to the speed in the breeding station 1, in particular of 7.2 l / min.
  • a filter system 14,15 is preferably used in two filter stages 1 1, 12, a hollow fiber membrane, in which in the first filter stage 1 1, a liquid content between 15 l / min and 50 l / min, in particular 30 l / min and in the second filter stage 12th between 0.3 l / min and 2 l / min, in particular 1, 0 l / min, is excreted.
  • a smaller amount of reaction medium 4 flows in as liquid is removed from the filter system 14, so that a concentration of the reaction medium 4 takes place.
  • the reaction medium 4 Concentration of the reaction medium 4, in particular of an algae and / or microorganisms 3-component of 30 g / l, the reaction medium 4 is transported from the collecting container 16 into the collecting container 17 in the further filter stage 12, preferably at the same time a cleaning process for the filter system 14th is started.
  • the filter stages Use 1 1, 12 with the same structure or to integrate a special filter stage for special algae or microorganisms 3.
  • the cleaning process can be triggered manually or automatically in a variety of ways.
  • a cleaning process is triggered, for example, when a predefinable inlet pressure of the reaction medium 4 over a defined period of time at the Filterystemi, 15 in particular at the entrance of the filter system 1 1, 12, preferably of 1, 5 applied bar. At this pressure, the collecting container 16 or 17 is disconnected from the rest of the line system via the valves 23 and at the same time the cleaning process for the filter system 14,15 started.
  • the cleaning process can also be triggered when a defined back pressure arises or a predefined concentration of the reaction medium 4 is reached, in which a transfer of the
  • an automatic cleaning process can be initiated, but for this purpose a corresponding warning signal or
  • the transfer of the reaction medium 4 from the collecting container 16 of the first filter stage 1 1 in the collecting container 17 of the second filter stage 12 is carried out at a rate between 20 l / min and 60 l / min, in particular with 25 l / min. The faster the reaction medium 4 is transferred, the less fresh
  • Reaction medium 4 flows from the breeding station 1 into the collecting container 16. This means that with a very rapid transfer, only a very small admixture of fresh, not very highly concentrated, reaction medium 4 takes place from the breeding station 1, so that the share for the transfer can be disregarded. It is essential that during the cleaning process, however
  • Reaction medium 4 from the breeding station 1 continues to flow continuously into the collecting container 16. That is, the discharge from the breeding station 1 and thus the supply to the harvesting module 2 is not interrupted, so that it is always ensured that in the breeding station 1, a corresponding
  • the collecting container 16 is dimensioned such that in the period of time as long as the cleaning process lasts, the reaction medium 4 from the breeding station 1 can be received by the collecting container 16. This means that when starting a cleaning process, the valves 23 are activated, at the same time the reaction medium 4 is transferred from the collecting container 16 of the first filter stage 1 1 in the further filter stage 12 and the collecting container 16 is filled with fresh incoming reaction medium 4 from the breeding station 1 again. In the process of cleaning the filter system 14, 15, the filter system 14, 15 is separated from the remaining components, in particular from the collecting container 16.
  • the reaction medium 4 remains twice to five times as long in the first filter stage 1 1 as in the second filter stage 12.
  • the reaction medium 4 only lingers between 2 and 3 hours. This is sufficient time to perform a cleaning process in the second filter stage 12 before again new reaction medium 4 is passed from the first filter stage 1 1.
  • the algae or microorganisms 3 are emptied and dried. The drying can take place by simple air drying or a drying plant, so that the algae or microorganisms 3 can be subsequently processed.
  • the cleaning process per se can be carried out in such a way that for cleaning the filter system 14,15, in particular the hollow fiber membrane a
  • the filter system 14,15 is constructed such that the
  • Hollow fiber membrane formed membrane filtration system is formed in a housing corresponding to a three-way valve, wherein the loaded with algae and / or microorganisms 3 reaction medium 4 is fed to an inlet and at the other end of the hollow fiber membrane via an outlet, the researcher passportde concentrated reaction medium 4, is conveyed. At the circumference between the two ends of the hollow fiber membrane, the separated nutrient solution is removed via a third outlet.

Abstract

L'invention concerne un procédé servant à l'obtention, en particulier à la récolte, d'algues et/ou de microorganismes (3) à partir d'un milieu réactionnel (4), par exemple servant à la récolte à partir d'une suspension constituée de microorganismes (3) et d'une solution nutritive, en particulier lors d'une culture et d'une production ou d'une culture hydroponique. Une concentration du milieu réactionnel (4) est effectuée par l'intermédiaire d'un système filtrant (14, 15) réalisé sous la forme d'une membrane à base de fibres creuses. Lors de la première étape, le milieu réactionnel (4) est amené en continu, en particulier également au cours d'un cycle de nettoyage pour le système filtrant (14, 15), à un réceptacle (16) d'une station de culture (1). Lors de la deuxième étape, le milieu réactionnel (4) traverse plusieurs étages filtrants (11, 12), en particulier au moins un autre étage filtrant afin d'augmenter la concentration du milieu réactionnel (4) en isolant du milieu réactionnel (4) le liquide, en particulier la solution nutritive.
PCT/AT2016/000039 2015-04-24 2016-04-22 Procédé servant à l'obtention, en particulier à la récolte, d'algues et de microorganismes WO2016168871A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ATA247/2015A AT517188A1 (de) 2015-04-24 2015-04-24 Verfahren zur Gewinnung, insbesondere zum Ernten, von Algen und Mikroorganismen
ATA247/2015 2015-04-24

Publications (2)

Publication Number Publication Date
WO2016168871A2 true WO2016168871A2 (fr) 2016-10-27
WO2016168871A3 WO2016168871A3 (fr) 2017-03-30

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AT (1) AT517188A1 (fr)
WO (1) WO2016168871A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018041859A1 (fr) 2016-08-30 2018-03-08 Ecoduna Ag Photobioréacteur muni d'un dispositif de maintenance mobile
FR3065966A1 (fr) * 2017-05-04 2018-11-09 Sas Alg&You ( Alg And You) Systeme de culture et de recolte de microalgues
CN115245700A (zh) * 2022-06-10 2022-10-28 生态环境部长江流域生态环境监督管理局生态环境监测与科学研究中心 一种浮游生物野外在线自动采集处理装置

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005143379A (ja) * 2003-11-14 2005-06-09 Tadashi Matsunaga 微生物の回収方法及び回収装置
DE202007004118U1 (de) * 2006-03-28 2007-09-20 Sartorius Biotech Gmbh Reaktoranlage zur Kultivierung phototropher Mikroorganismen

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005143379A (ja) * 2003-11-14 2005-06-09 Tadashi Matsunaga 微生物の回収方法及び回収装置
DE202007004118U1 (de) * 2006-03-28 2007-09-20 Sartorius Biotech Gmbh Reaktoranlage zur Kultivierung phototropher Mikroorganismen

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018041859A1 (fr) 2016-08-30 2018-03-08 Ecoduna Ag Photobioréacteur muni d'un dispositif de maintenance mobile
US11098276B2 (en) 2016-08-30 2021-08-24 Beco Invest B.V. Photobioreactor having movable maintenance device
US11976260B2 (en) 2016-08-30 2024-05-07 Ecoduna Ag Photobioreactor and method for the cultivating of microalgae
FR3065966A1 (fr) * 2017-05-04 2018-11-09 Sas Alg&You ( Alg And You) Systeme de culture et de recolte de microalgues
CN115245700A (zh) * 2022-06-10 2022-10-28 生态环境部长江流域生态环境监督管理局生态环境监测与科学研究中心 一种浮游生物野外在线自动采集处理装置
CN115245700B (zh) * 2022-06-10 2023-03-24 生态环境部长江流域生态环境监督管理局生态环境监测与科学研究中心 一种浮游生物野外在线自动采集处理装置

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