WO2016159423A1 - Additive composition for livestock feed, containing pozzolan, and use thereof - Google Patents

Abstract

The present invention relates to an additive composition for livestock feed, containing pozzolan, and specifically, the present invention provide a novel use of an additive composition for livestock feed, the composition containing, as an active ingredient, pozzolan, which has an excellent antibiotic effect to substitute for a conventional antibiotic agent, enhances the immune function, and removes bad smells, and thus is very suitable for livestock feed.

Description

Additives composition for livestock feed comprising pozzolan and use thereof

The present invention relates to an additive composition for livestock feed and its use comprising Pozzolan as an active ingredient.

Organic livestock refers to livestock that has not been fertilized or genetically modified for livestock production without chemical fertilizers, pesticides, and genetically modified feeds. In addition, antibiotics, growth hormone, and animal origin It is not an intensive factory-type breeding that does not use artificial synthetic additives such as by-product feeds or animal medicines, but it is a natural method of breeding, processing, Means the distribution, evaluation, and labeling of livestock and their livestock products.

The certification of organic livestock includes the production and distribution of organic livestock as well as the final product, the livestock food. The most difficult part of implementing organic livestock is the coping and prevention of diseases when breeding without antibiotics. This requires the administration of feed additives with immunity and antimicrobial / antiviral activity.

In 2010, the global organic food market grew by 12.4% year-on-year to US $ 59,341 million, and in 2015, it is expected to reach US $ 88,069.3 million, an increase of 48.4% from 2010. The global organic food market is dominated by Europe (Germany, UK), North America, Australia, etc. In particular, the United States is the largest market in the world, surpassing Europe, accounting for 49% of total global organic food revenue in 2009. . The demand for organic food is concentrated in Western countries such as Europe and America. In particular, with the rise of eco-friendly organic livestock, the EU has stipulated that since 2005, 100% organic feed should be used for feed used for organic livestock production and antibiotics are no longer allowed (Commission Regulation EC 2277, 2003). It has been in effect since March (European Union Commission, 2005). In Germany, the word bio can be used only for products that are certified organic, and the word organic cannot be used because some certified additives, such as sweet water, salt and yeast, are not recognized as agricultural products. In Germany, more than 95% of the raw materials must be obtained from organic farming and ban on the use of genetically modified ingredients.

As a result, developed countries have made great efforts to develop and implement effective measures for implementing organic livestock. However, while the demands of consumer organizations and NGOs for organic livestock are very high, especially in the US and the EU, the implementation of organic livestock is still small and lagging behind the international standardization of food.

In the case of Korea, the Korean Food Standard for Organic Livestock, which is appropriate for our situation, is applied based on the Codex's Organic Livestock Standard, and the most economical scale and breeding system for organic livestock is developed in Korea. It is at the time of implementation.

In Korea, the scale of implementation of organic livestock is very small, and there is a lack of international standardization of food. Therefore, based on CODEX's Organic Livestock Code, Korea has enacted the Korean-style organic livestock regulation, which is suitable for our situation, as the enforcement rule of the Eco-Friendly Agriculture Promotion Act in 2001. It is at this point.

The certification criteria for organic livestock raising in Korea is the transition period (12 months after stocking of cattle, 6 months after pigs). Use only acceptable substances and do not use antibiotics or growth accelerators. According to the Ministry of Agriculture, Forestry and Forestry Notice No. 2004-72, it was limited to 18 species (9 anticoccidial agents, 9 growth promoters) from December 2007. In 2012, the use of antibiotics as a growth accelerator for livestock is completely prohibited.

In particular, the abuse of antibiotics in humans and livestock has led to the emergence of potent super bacteria that show selective resistance to antibiotics in vivo. Horizontal migration of antibiotic resistance genes in the environment (Shakibaie et al., 2009), fish (Matyar et al., 2004) and livestock food (Toroglu et al., 2009; Jones et al., 2002) The emergence of Methicillin-resistant Staphylococcus aureus (MRSA), Vancomycin-resistant enterococcus (VRE) in resistant bacteria and hospitalized patients (Cosgrove et al., 2005) has become a serious social issue (Toroglu et al., 2005; Perl). , 1999). In plant-intensive livestock farming, there is an urgent need to develop new antibiotic substitutes that can replace antibiotics to reduce the damage and loss of feed antibiotics and to ensure continued animal productivity (Dibner and Richards, 2005). Without the concern about the emergence of antibiotic resistant strains for humans and animals, the development of antimicrobial growth promoters from natural products that can replace antibiotics for livestock is actively underway.

Currently, the use of antibiotics and antimicrobials in livestock is becoming more regulated due to the safety issues of livestock products. In particular, the use of antibiotics added to formulated feeds will be tightened in the future. Avoid long-term use of antibiotics. In addition, antibiotic-resistant pathogens appear due to the abuse of antibiotics, which requires the development of alternative antimicrobial substances.

Therefore, there is a need for the development of new materials that can exert anti-inflammatory, anti-inflammatory, growth and antimicrobial / antiviral effects that can prevent or treat livestock diseases while excluding antibiotics.

The inventors of the present invention can replace antibiotics and provide an antimicrobial composition for feed, and in particular, it is possible to suppress harmful bacteria and improve immune function for livestock feed, and to improve feed demand and feed for livestock feed. It is an object to provide an antimicrobial composition of a new material. It is also an object of the present invention to provide a feed additive, a livestock feed comprising the same, and a novel immune enhancing method.

 According to one embodiment of the present invention, it provides an antimicrobial composition for livestock feed comprising pozzolan as an active ingredient. The pozzolanic may be a combination of any one or more of silica, aluminum, aluminum oxide (Al 2 O 3), iron, (Fe), ferric trioxide (Fe 2 O 3), and germanium (Ge). Preferably the pozzolane is a composition having a silica content of at least 70% by weight. The pozzolane is a composition in which the content ratio of silicon and silicon dioxide (SiO 2) in silica is in the range of 1: 2 to 1: 3. In addition, the pozzolan may be 80% by weight or more of silica and aluminum oxide. It is preferred to be in the powder phase in the pozzolane. The pozzolanic is preferably mixed in a ratio of 0.01 to 0.70% by weight based on the total weight of the feed. The livestock may be any one of cows, chickens, pigs, horses, goats, ducks, geese, dogs, cats, and rabbits.

According to another aspect of the present invention, there is provided a livestock feed additive containing the antimicrobial composition as an active ingredient. The pozzolanic is preferably mixed in a ratio of 0.01 to 0.70% by weight based on the total weight of the feed, the livestock may be any one of cow, chicken, pig, horse, goat, duck, goose, dog, cat, rabbit. have.

According to another aspect of the present invention, there is provided a livestock feed comprising the livestock feed additive.

According to another aspect of the present invention, there is provided a method for enhancing immunity of a livestock, wherein the antimicrobial composition or the livestock feed additive is administered to a livestock.

Pozolan for livestock feed according to the present invention is a very suitable material for feed of organic livestock, such as odor removal while enhancing the immune function and have an antimicrobial / antiviral effect. It improves the absorption and utilization of nutrients and has a weight gain effect, which in turn can improve meat quality and promote growth. Livestock It can be applied to a variety of livestock feed, and can replace antibiotics with various side effects, so it is expected to satisfy the strict specifications of countries around the world including Korea.

1 to 4 are photographs showing the growth inhibitory or promoting effect of each of the strains Salmonella typhimurium, Escherichia coli, Clostridium butyricum, Lactobacillus casei.

5 is a graph comparing the growth of the strain according to the medium. Control group (medium 1), SP extract (medium 4), SP3% (medium 2), SP5% (medium 3) is shown.

6 and 7 are photographs showing growth inhibition of strains MRSA (Staphylococcus aureus) and VRE (Enterococcus), respectively.

Figure 8 is a graph comparing the growth of the strains (MRSA and VRE). Control group (medium 1), SP extract (medium 4), SP3% (medium 2), SP5% (medium 3) is shown.

 9 is a graph comparing the 35-day shipping weight of the broiler of Example 3. FIG.

Figure 10 is a graph comparing the weight gain rate by growth period of the broiler of Example 3.

Figure 11 is a graph comparing the feed efficiency of the broiler of Example 3.

Control group (T1), SP 0.3% (T2), SP 0.5% (T3), SP 0.7% (T4) are shown.

12 is a graph comparing the conductor ratio of the broiler of Example 3. FIG.

FIG. 13 is a graph comparing tracheal weight at week 3 of the broiler of Example 3. FIG.

14 is a graph comparing tracheal weight at week 5 of the broiler of Example 3. FIG.

FIG. 15 is a graph comparing the weight of immune organs of the broiler of Example 3 at week 3. FIG.

16 is a graph comparing the weight of immune organs of the broiler of Example 3 at week 5. FIG.

17 is a graph comparing blood IgG content of broilers of Example 3. FIG.

18 is a graph comparing the concentration of powdered microorganisms in a broiler of Example 3. FIG.

19 and 20 are graphs comparing the contents of cecum organic acid of the broiler of Example 3.

Control group (T1), SP 0.3% (T2), SP 0.5% (T3), SP 0.7% (T4) are shown.

21 is a graph comparing fatty acid content of broiler of Example 3.

22 is a graph comparing the ammonia content of broilers of Example 3.

Figure 23 is a graph comparing the hydrogen sulfide content of the broiler of Example 3.

24 is a graph comparing end weights of piglets of Example 4. FIG.

Control (T1), antibiotics 0.2% (T2), pozzolane 0.3% (T3), pozzolane 0.5% (T4), pozzolane 0.7% (T5).

25 is a graph comparing the daily weight gain of piglets of Example 4.

Figure 26 is a graph comparing the daily feed intake of piglets of Example 4.

Figure 27 is a graph comparing the feed demand rate of piglets of Example 4.

28 is a graph comparing blood analysis results of piglets of Example 4. FIG.

29 is a graph comparing the concentration of powdered microorganisms in piglets of Example 4. FIG.

30 and 31 are graphs comparing the contents of cecum organic acid in piglets of Example 4.

Control (T1), antibiotics 0.2% (T2), SP 0.3% (T3), SP 0.5% (T4), SP 0.7% (T5).

 The present invention provides an antimicrobial composition for livestock feed comprising pozzolan as an active ingredient.

The pozzolan mineral is composed of volcanic ashes and is known as a type of feldspar. It is known as an orphan created during the Cretaceous period, and its main mountain is a mineral derived from Pojori village near Bari Island in Italy, and the famous Colosseum Stadium, which was already famous in Roman times around 2nd century AD, and Pandeon. It has been found by scholars to use pozzolanic, which has been used in place of cement in buildings and has been preserved in its original form for years without cracking and corrosion. Other distribution areas are produced only in extremely limited regional countries such as the United States, the Mountain Region, Malaysia, and India, and the unusual ones are the fact that there is no germanium or far-infrared emission effect other than Italy and Korea. The same vein does not exist at all.

In the past, pozzolan was added to the base material of concrete manufacturing and used as a mixed material to give special performance to concrete or to improve its properties.Inorganic solidifying material for solidifying waste is usually cement-reactive, so that inorganic sludge of appropriate moisture content Suitable for solidifying (containing heavy metals). In addition, pozzolan (Pozzolan) having a good affinity with cement is most commonly used to control the sludge moisture content and cement replacement solidification aid, and the cost is relatively low.

Pozzolanic is at least one selected from the group consisting of volcanic ash, tuff, siliceous silica, diatomaceous earth and natural rock, and is preferably in powder form. In addition, the main component may be silica-alumina or silica, and preferably pozzolan is a combination of any one or more of silica, aluminum, aluminum oxide (Al 2 O 3), iron, (Fe), ferric trioxide (Fe 2 O 3), and germanium (Ge). Composition. Also preferably, pozzolanic is a composition having a silica content of at least 70% by weight. The pozzolane is a composition in which the content ratio of silicon and silicon dioxide (SiO 2) in silica is in the range of 1: 2 to 1: 3. The pozzolane preferably has a content of silica and aluminum oxide of 80% by weight or more. It is preferred to be in the powder phase in the pozzolane.

The term “comprising” of the present invention is not limited to including, and does not exclude, for example, other additives, components, indices or steps.

By “antimicrobial” is meant the ability to reduce, prevent, inhibit or eliminate the growth or survival of microorganisms at any concentration. The antimicrobial composition may mean the same as an antibiotic, which is a generic term for an antimicrobial agent, and may mean the same as an antimicrobial agent, a fungicide, a preservative, a preservative or an antimicrobial agent, preferably Gram-positive bacteria, Gram-negative bacteria, fungi (yeast and mold) Means a substance capable of inhibiting or inhibiting the development and life functions of at least one microorganism selected from the group consisting of, that is, antibacterial and antifungal substances.

The pozzolanic may be added to the feed without content limitation, but is preferably mixed in a ratio of 0.01 to 0.70% by weight based on the total weight of the feed. More preferably, it is 0.3 to 0.7 weight%, More preferably, it is 0.5 weight%.

The livestock may be cattle, chickens, pigs, horses, goats, ducks, geese, dogs, cats or rabbits, preferably chicken or pigs, but is not limited thereto.

According to another aspect of the present invention, there is provided a livestock feed additive containing the antimicrobial composition as an active ingredient. The livestock feed additive may be used as it is or may be added to known carriers, stabilizers, etc., such as grains and by-products allowed for livestock, and organic acids such as citric acid, fumaric acid, adipic acid, lactic acid, and malic acid, if necessary. Phosphates such as sodium phosphate, potassium phosphate, acid pyrophosphate, and polyphosphate (polyphosphate), or natural substances such as polyphenols, catechins, alpha-tocopherols, rosemary extracts, vitamin C, green tea extracts, licorice extracts, chitosan, tannic acid, and phytic acid. Antioxidants, antibiotics, antibacterial agents and other additives may be added, and the shape thereof may be in a suitable state such as powder, granules, pellets, suspensions, etc. It can be mixed and supplied to.

The pozzolanic is preferably mixed in a ratio of 0.01 to 0.70% by weight based on the total weight of the feed, the livestock may be any one of cow, chicken, pig, horse, goat, duck, goose, dog, cat, rabbit. have.

According to another aspect of the present invention, there is provided a livestock feed comprising the livestock feed additive. In the case of producing livestock feed in the present invention, the additives may be cereals, for example crushed or ground wheat, oats, barley, corn and rice; Vegetable protein sources based on rapeseed, soybean and sunflower seeds; Animal protein sources; molasses; And dry ingredients consisting of milk products such as various milk powders and whey powders. After mixing with all the dry ingredients, the liquid ingredients and the ingredients which become liquid after heating can be added. The liquid component may consist of lipids, such as fats, for example vegetable fats, and / or carboxylic acids, for example fatty acids, optionally liquefied by heating. After complete mixing, a powder or particulate concentration is obtained depending on the degree of polishing of the components. In order to prevent segregation during storage, water should preferably be added to the animal feed, which is subsequently processed by conventional pelletization, extension or extrusion processes. Any additional water can be removed by drying. If desired, the resulting particulate animal feed can be ground to smaller particle sizes.

According to another aspect of the present invention, there is provided a method for enhancing immunity of a livestock, wherein the antimicrobial composition or the livestock feed additive is administered to a livestock. Preferably the pozzolane is mixed at a ratio of 0.01 to 0.70% by weight based on the total weight of the feed, more preferably 0.3 to 0.7% by weight, even more preferably 0.5% by weight. The livestock may be cattle, chickens, pigs, horses, goats, ducks, geese, dogs, cats or rabbits, preferably pigs, but is not limited thereto.

Hereinafter, the operation and effects of the invention will be described in more detail with reference to specific examples of the invention. However, these embodiments are only presented as an example of the invention, whereby the scope of the invention is not determined.

Example 1 Preparation of Pozolan and Identification of Ingredients

 Pozzolane was supplied with powdered products from Morningstar Co., Ltd. and analyzed by ingredients of KIST's analysis institute, and as a result, various Si and Ge components were identified as shown in Tables 1 and 2 below. It became.

In order to test the antimicrobial and growth effects of the various livestock of the pozzolan, a sample was prepared by mixing various amounts of pozzolanic or pozzolanic extract in a raw material medium or feed.

<Example 2>

In order to test the growth promoting or inhibitory effect on various beneficial or harmful bacteria, the growth of various strains was measured through the following experimental design.

2-1. Experimental strain

Intestinal strains received four general strains from Korea Food Research Institute (KFRI), and antibiotic resistant strains received two resistant strains from Seoul Women's University resistant strain bank (KNRRC). Table 3 below lists the scientific name and strain accession number of the strain.

2-2. Culture method and medium

Distilled water medium 1, which is a control group, was sterilized after adding distilled water to the raw material of the medium without adding pozzolan, diluted with strain 10 ⁻⁷ , and then inoculated with 100 uL. Distilled water medium 2 and 3 of the experimental group were mixed with 3.0 and 5.0% of distilled water and pozzolane, respectively, and sterilized in the raw material, and diluted with strain 10 ⁻⁷ , respectively, and then inoculated with 100 uL. Medium 4 with pozzolanic extract to a different experimental groups was mixed and filtered through filter paper (No.1) 2-sheet after 9 hours at 115 ℃ overnight political the pozzolan and 100g distilled water 500mL sterilization the medium was filtered and diluted with strain ^10-6 100 μL was then inoculated. The medium of the control group and the experimental group is shown in Table 4 below.

After incubating at 37 ℃ for 24 hours, the number of bacteria was measured. However, Lactobacillus casei was incubated for 48 hours at 37 ℃. Table 5 below summarizes the characteristics of the strains and the medium used for each strain.

2-3. Culture result of strain

The growth results of the culture according to the strain according to the culture method are shown in Table 6 below. Single unit 1-4 strain is a ^-7 Log10 cfu, single strain 5-6 is Log10 cfu ^-6. CFU (Colony Forming Unit) is a microbial colony forming unit, which is a unit of colonies (colony = mass, colony) that grows to a size that is visible to each microbe by growing microorganisms that are hard to be seen under the proper conditions.

Photos showing growth inhibition or promotion according to the media of the respective strains are shown in FIGS. 1 to 4 and 6 to 7, respectively. Comparison graphs between the strains are shown in FIGS. 5 and 8. As can be seen from the results, the growth of Lactobacillus casei promoted the growth of beneficial bacteria ( Salmonella typhimurium, Escherichia coli, MRSA, VRE) in the medium added with pozzolanic or pozzolanic extract. The effect was confirmed.

<Example 3>

In order to test the antimicrobial growth promoting effect on broiler broiler (broiler) was selected and various effects were measured through the experimental design as follows.

3-1. Experimental Animal and Experimental Design

Incubated male broiler 1 day-old male broilers subjected to sexual identification of Ross system (Ross 308) were randomly placed in four treatments X 4 repetitions (25 per repetition). Experimental treatments were divided into T1 (control), T2 (pozolan 0.3%), T3 (pozolan 0.5%), T4 (pozolan 0.7%).

3-2. Experiment feed and specification management

Experimental feeds were formulated mainly to corn or soybean meal to meet or exceed the nutrient requirements of broilers as set forth in the US NRC specification standard (1994), and the formulated feed production according to the addition level of pozzolan reduced the amount of crude protein and metabolic energy. The content was adjusted to the same level.

3-3. Specification Performance and Conductor Characteristics

The growth capacity of each stage according to the growth of broiler, that is, feed intake, weight gain and feed efficiency, were measured, respectively, and the results are shown in Table 7 below. Feed efficiency was expressed as feed intake divided by weight gain in a given period. The dressing percent was calculated as the ratio of carcass weight (weight excluding feathers, blood, head, legs and viscera) to live weight at day 35. The results are shown in Table 8 below. The weights of liver, proximal and immune organs (thymus, spleen, F sac) were measured at 3 weeks and 5 weeks, respectively. Table 10 is the analysis of lipids, blood sugar, AST, ALT, and the like through blood analysis.

9 is a graph comparing the 35-day shipping body weight according to the pozzolanic content showed the largest increase rate in the case of 0.5% content of pozzolanic T3. Figure 10 is a comparison of the weight gain rate by growth period, the highest increase rate of pozzolanic 0.5% content at each time period. FIG. 11 shows a comparison of feed efficiency, and showed similar results for pozolan 0.3% and 0.7%, and slightly increased for 0.5% T3. FIG. 12 shows a somewhat higher increase in T3, comparing the conductor rates. FIG. 13 is a graph comparing the increase in tracheal weight at 3 weeks, the largest increase in T3 followed by the highest increase in the order of T2 and T4. Figure 14 is a graph comparing the increase in tracheal weight at 5 weeks, which also showed a slightly higher increase in T3, and the other T2 and T3 were similar to the control group T1. Figure 15 shows the weight of the immune system at 3 weeks, showing a significant increase in T3 indirectly showing that the duration of immunity is enhanced. Figure 16 shows the weight of the immune organs at week 5, which also showed a markedly high increase in T3.

3-4. Blood immune substances

Blood immunoassay was determined by ELISA (enzyme-linked immunosorbent assay, Bethyl laboratories., Inc., USA) (Constantinoiu et al., 2007). The results are shown in Table 11.

Figure 17 is a graph comparing the content of IgG as a blood immune material showed the largest increase in T3 and in order of T4 and T2 confirmed that the pozzolanic can increase the secretion of immune material.

3-5. Minute microorganism

Three minutes before the end of the experiment, the mixture was collected and mixed with phosphate buffered saline (popozolan horus buffered saline) and diluted 10-fold (w / v). Samples diluted to 10 ² -10 ⁸ concentrations were dispensed into sterile plate media. After 48 hours of incubation at 37 ° C, the number of colonies was examined as a microbial counter, and a commercial log was taken as the number of colonies per log (log ₁₀ CFU, colony forming unit / g of feces) and the results are shown in Table 12.

Fig. 18 is a graph comparing the change patterns of the microorganisms, and the beneficial bacteria showed the highest concentration in T3 for Lactobacillus , and the high inhibitory effect for the harmful bacterium E. Coli, Salmonella .

3-6. Cecum organic acid

The cecals were collected from the sacrificed chickens and the concentrations of acetate, propionate, butyrate, isobutyrate, valerate and isovalerate were measured by a gas chromatographic system (model GC-15A, Shimadzu Corp., Kyoto, Japan). It was.

Figures 19 and 20 compare the cecum organic acid, although the content of some organic acids decreased, but the overall short-chain organic acid (SCFA, Sohrt chain Fatty acid) showed the largest increase rate in T3 and increased in the treatment group containing pozzolanic Showed.

3-7. Chicken Fatty Acid

Lipids were extracted from chicken leg meat and injected into a gas chromatographic system (model GC-15A, Shimadzu Corp., Kyoto, Japan) to analyze fatty acids. The results are shown in Table 14.

21 is a comparison of chicken fatty acids, saturated fatty acid (SFA) was decreased in the treatment group of pozzolanic, unsaturated fatty acid (UFA) was increased in the treatment group of pozzolanic. Unsaturated fatty acid / saturated fatty acid (UFA / SFA) levels were the highest in T3 and overall increased in the pozzolan-treated group. Eventually, it was confirmed that pozolan increases the content of unsaturated fatty acids, which can increase the nutritional efficacy of broilers and improve their quality.

3-8. Stepping odor

Ammonia and hydrogen sulfide concentrations were measured with a gas indicator (Gas Indicator AP-20, Axis Sensitive Co. Ltd, Japan). After inhaling the gas according to the manufacturer's manual, the displayed value was recorded and compared with the control, and presented as shown in Table 15 below.

22 and 23 are graphs comparing the contents of ammonia and hydrogen sulfide, showing a tendency to decrease overall in the treatment group containing pozzolanic, in particular, the largest decrease in T4.

<Example 4>

In order to test the antimicrobial growth promoting effect on weaning piglets, we selected hybrid weaning piglets and measured various effects through experimental design as follows.

4-1. Experimental Animal and Experimental Design

Under standard environmental conditions, 300 rats of three-way hybrid (Landrace * Yorkshire) * Duroc weaning pigs (average body weight 121.20 kg) were used and randomly placed in a total of 20 treatments per 5 treatments 3 replicates. The experimental groups were divided into T1 (no additive group), T2 (antibiotic lincomycin 0.2%), T3 (pozolan 0.3%), T4 (pozolan 0.5%), T5 (pozolan 0.7%).

4-2. Experiment feed and specification management

Experimental feeds were formulated mainly on corn and soybean meal to meet or exceed the nutrient requirements of pigs suggested by the US NRC specification (1994). Crude protein and metabolic energy content were adjusted to the same level.

4-3. Growth capacity and diarrhea frequency

Initiation and 30 days per piglet to investigate the growth capacity of weaners, ie, average daily feed intake (ADFI), average daily weight gain (ADWG) and feed conversion ratio (FCR). Weight and feed intake were measured and the frequency of diarrhea in weaned piglets was investigated. Feed demand was expressed as weight gain divided by feed intake. Identify the number of individuals with evidence of benign diarrhea around the anus twice a day for all piglets in each piglet. Diarrhea frequency was measured by the 9-point method and diarrhea frequency was recorded from 9 points to 0 points when the anus periphery of all individuals showed signs of benign diarrhea. The results are shown in Table 16 below. Table 17 shows the analysis of lipids and blood glucose through blood analysis.

24 to 27 are graphs comparing end weight, daily weight gain, daily intake, and feed demand rate, respectively, showing a high increase rate in the treatment group containing pozzolanic, especially the highest value in the group containing 0.5% pozzolanic. Showed.

FIG. 28 is a graph comparing the levels of lipids and blood glucose as a result of blood analysis, showing an increase in the treatment group containing pozzolanic. This is higher than the antibiotic treatment group, demonstrating the replacement effect of antibiotics.

4-4. Blood immune substances

Blood immunoassay was measured by ELISA (enzyme-linked immunosorbent assay, Bethyl laboratories., Inc., USA) (Constantinoiu et al., 2007) and the results are shown in Table 18.

In the graph comparing the immune material in Figure 28 it was confirmed that in the T4 treated group showed a higher content of the immune substance than the T3 group treated with antibiotics, it was possible to enhance the immune function without administering antibiotics.

4-5. Minute microorganism

Three minutes before the end of the experiment, the mixture was collected and mixed with phosphate buffered saline (popozolan horus buffered saline) and diluted 10-fold (w / v). Samples diluted to 10 ² -10 ⁸ concentrations were dispensed into sterile plate media. After 48 hours of incubation at 37 ° C, the number of colonies was examined as a microbial counter, and the commercial log was taken as the number of colonies per log (log ₁₀ CFU, colony forming unit / g of feces) and the results are shown in Table 19 below.

Fig. 29 is a graph comparing the microorganisms with beneficial bacteria showed the highest concentration in T3 in the case of Lactobacillus , and showed higher inhibitory effect than the antibiotics in the case of harmful bacterium E. Coli, Salmonella .

4-6. Cecum organic acid

The cecals were collected from sacrificial weaners and the concentrations of acetate, propionate, butyrate, isobutyrate, valerate and isovalerate were measured by a gas chromatographic system (model GC-15A, Shimadzu Corp., Kyoto, Japan) and the results are shown in Table 20. .

30 and 31 are graphs comparing the contents of organic acids. The T4 group containing 0.5% of the total short chain organic acid (SCFA) containing pozzolanic showed a higher increase rate than the T2 group treated with antibiotics, and contained pozzolanic as a whole. The treatment group showed a tendency to increase. Thus, it was confirmed that pozolan induced an increase in the organic acid of the cecum.

Claims (15)

1. Animal feed additive composition comprising a pozzolan as an active ingredient.
2. The method of claim 1,

   The pozzolanic is a composition characterized in that the combination of any one or more of silica, aluminum, aluminum oxide (Al2O3), iron, (Fe), ferric trioxide (Fe2O3), germanium (Ge).
3. The method of claim 1,

   The pozzolanic composition is characterized in that the silica content of more than 70% by weight.
4. The method of claim 1,

   The pozzolanic is a composition, characterized in that the content ratio of silicon and silicon dioxide (SiO 2) in silica is in the range of 1: 2 to 1: 3.
5. The method of claim 1,

   The pozzolanic composition is characterized in that the content of silica and aluminum oxide of more than 80% by weight.
6. The method of claim 1,

   The pozzolanic composition is characterized in that the powder phase (powder phase).
7. The method of claim 1,

   The pozzolanic is characterized in that the composition is mixed in a ratio of 0.01 to 0.70% by weight based on the total weight of the feed.
8. The method of claim 1,

   The livestock is a composition, characterized in that any one of a cow, chicken, pig, horse, goat, duck, goose, dog, cat, rabbit.
9. Animal feed additive containing the composition of claim 1 as an active ingredient.
10. The method of claim 9,

    Pozzolan is a livestock feed additive, characterized in that mixed with 0.01 to 0.70% by weight based on the total weight of the feed.
11. The method of claim 9,

    The livestock feed additives, characterized in that any one of cows, chickens, pigs, horses, goats, ducks, geese, dogs, cats, rabbits.
12. Animal feed comprising the animal feed additive of claim 9.
13. The method of claim 1, comprising administering the composition of claim 1 or the animal feed additive of claim 9 to the animal.
14. The method of claim 13,

    The pozzolanic is characterized in that the mixture of 0.01 to 0.70% by weight based on the total weight of the feed.
15. The method of claim 13,

    The livestock is any one of a cow, chicken, pig, horse, goat, duck, goose, dog, cat, rabbit.

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