WO2016153199A1 - Appareil pour retirer de la graisse et appareil pour séparer des cellules - Google Patents

Appareil pour retirer de la graisse et appareil pour séparer des cellules Download PDF

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Publication number
WO2016153199A1
WO2016153199A1 PCT/KR2016/002448 KR2016002448W WO2016153199A1 WO 2016153199 A1 WO2016153199 A1 WO 2016153199A1 KR 2016002448 W KR2016002448 W KR 2016002448W WO 2016153199 A1 WO2016153199 A1 WO 2016153199A1
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WIPO (PCT)
Prior art keywords
tip
fat
hand piece
cells
unit
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Application number
PCT/KR2016/002448
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English (en)
Korean (ko)
Inventor
김지환
이성형
Original Assignee
김지환
이성형
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020150130446A external-priority patent/KR20160115656A/ko
Application filed by 김지환, 이성형 filed Critical 김지환
Publication of WO2016153199A1 publication Critical patent/WO2016153199A1/fr
Priority to US15/714,073 priority Critical patent/US20180021599A1/en
Priority to US17/970,833 priority patent/US20230039796A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B18/00—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N7/00—Ultrasound therapy

Definitions

  • Various embodiments of the present invention relate to a fat removal device for removing subcutaneous fat using ultrasound and a cell separation device for separating cells from fat cells.
  • liposuction or liposuction is a plastic surgery to remove the fat layer accumulated excessively in the human body.
  • the fat removal surgery can easily remove the fat of the area difficult to remove by exercise, diet and the like as needed.
  • exercise, dietary methods, etc. only reduce the size of fat cells, it is difficult to prevent thickening of the fat layer later, while liposuction reduces the number of fat cells.
  • a physiological saline solution containing a local anesthetic, a vasoconstrictive agent, etc. is infused into a desired fat layer to inflate the fat layer to several times its original thickness, and then, using the cannula, the fat is removed in the same manner as dry plastic surgery. That's the way it is.
  • the cannula includes a tip.
  • the wet plastic surgery because the thickness of the fat layer to be treated is inflated up to several times the original thickness, it is possible to increase the precision of the procedure, compared to dry plastic surgery, such as reducing the amount of bleeding during surgery by reducing capillaries by the vasoconstrictor It has many advantages.
  • the wet plastic surgery like dry plastic surgery, has to move the tip of the cannula on the rough surface back and forth to separate and destroy the fat cells, and thus cannot solve problems such as pain of the patient and excessive labor of the operator.
  • Such ultrasonic fat removal apparatus uses a device that converts ultrasonic electron energy into mechanical energy called vibration.
  • the tip of the cannula which generates ultrasonic vibration using piezoelectric crystals, which vibrates when an alternating current is provided, is inserted into the fat layer and vibrated. Bubbles are generated in the fat layer by ultrasonic vibration of the tip, and fat is separated by cavitation, micromechanical destruction, thermal effect, etc. by the generation of the bubbles, and by removing such fat Will be removed.
  • the thermal effect serves to dissolve fat.
  • the conventional ultrasonic fat removal device has a disadvantage in that a cannula that dissolves fat using ultrasonic waves to remove subcutaneous fat generates a lot of heat at the tip portion of the cannula for thermal effects, causing burns to the skin and muscles of the user. Not only this. After the fat removal procedure, swelling occurred due to pain and burn inflammation of the patient.
  • the tip portion of the hand piece is configured to cool the heat generated during the vibration by ultrasonic waves, thereby preventing burns due to the heat generation of the tip on the skin layer and the muscle layer of the patient.
  • the present invention provides a fat removing device that can prevent swelling in pain and cosmetic inflammation of a patient after the procedure.
  • stem cell-related isolation methods that help treat cells by separating cells from human fat or other tissues are a rapidly growing field.
  • the method of separating stem cells from human fat cells has previously used a method of dissolving cells and extracting byproducts by using enzymes (collagenase).
  • enzymes unlike the acidic or alkaline solutions, enzymes (collagens) are not neutralized and have the same toxicity as the enzymes themselves. Unneutralized enzymes can dissolve other proteins and fats in the human body, and conventional stem cell separation equipment has a disadvantage in that it takes a lot of time to remove the remaining enzymes by washing these enzymes (collagenase) several times.
  • the cell separation method using an existing enzyme contains fat and cells collected from the human body in a container of a certain size and adds the enzyme (collagenase) to the temperature between 38 degrees and 39 degrees, similar to the human body. Dissolve cells for 40 minutes at. After that, the dissolved cells are centrifuged to separate stem cells and other cells.
  • the conventional cell separation method dissolves rotten fat cells and cells with enzymes (collagenase) for 40 minutes after the enzyme (collagenase) is neutralized with normal cells. At this time, the time required may take 20 minutes and 1 hour or more. This is the time that should be used because of the enzyme.
  • ultrasonic cells are designed to reduce the time to neutralize and neutralize the existing enzyme (collagenase).
  • an electronic fat removing apparatus using ultrasonic waves may include: a fat removing body part; A hand piece having an ultrasonic generator electrically connected to the fat removal main body; A tip part provided on the hand piece; A temperature sensor unit provided at the tip unit to sense a temperature of the tip unit; A peristaltic pump part provided in the fat removing body part and connected to the hand piece and transferring coolant to the tip part; And a controller configured to receive the signal sensed by the temperature sensor unit and to drive the peristaltic pump unit at a preset temperature.
  • an electronic fat removing apparatus using ultrasonic waves may include: a fat removing body part; A hand piece having an ultrasonic generator electrically connected to the fat removal main body; A tip part provided on the hand piece to receive and vibrate ultrasonic vibration of the ultrasonic generator; A temperature sensor unit provided at the tip unit to sense a rising temperature of the tip unit according to ultrasonic vibration; A peristaltic pump unit provided in the fat removing body unit and connected to the hand piece and transferring a coolant to the tip unit to cool the elevated temperature of the tip unit; A cooling unit connected to the peristaltic pump unit to supply the cooling water; And a controller configured to receive the signal sensed by the temperature sensor unit and to drive the peristaltic pump unit at a preset temperature.
  • a cell separation device may include: a hand piece having an ultrasonic wave generator; A tip part provided on the hand piece; And a storage cap containing fat cells and cells, wherein the storage cap is coupled to the hand piece and inserts the tip into the storage cap to dissolve and separate the fat cells and the cells by ultrasonic waves of the ultrasonic generator.
  • a hand piece having an ultrasonic wave generator A tip part provided on the hand piece
  • a storage cap containing fat cells and cells wherein the storage cap is coupled to the hand piece and inserts the tip into the storage cap to dissolve and separate the fat cells and the cells by ultrasonic waves of the ultrasonic generator.
  • FIG. 1 is a view showing the configuration of a fat removal apparatus according to various embodiments of the present invention.
  • FIG. 2 is a view illustrating an operating state of a fat removing device according to various embodiments of the present disclosure.
  • Figure 3 is a side cross-sectional view showing an operating state of the fat removal device according to various embodiments of the present invention.
  • FIG. 4 is an enlarged side cross-sectional view of portion A of FIG.
  • Figure 5 is a side cross-sectional view showing a storage cap of the cell separation apparatus according to various embodiments of the present invention.
  • Figure 6 is a side cross-sectional view showing a coupling state of the tip portion provided in the storage cap and the hand piece of the cell separation apparatus according to various embodiments of the present invention.
  • Figure 7a is a view showing a state before inserting the tip of the hand piece in a storage cap containing 25cc fat cells and cells according to various embodiments of the present invention.
  • FIG. 7B is a view illustrating a process of melting a cell after inserting a tip of a hand piece into a storage cap containing 25 cc of fat cells and cells according to various embodiments of the present disclosure.
  • FIG. 8 is a view showing a state in which cells are separated using a centrifuge for dissolved cells according to various embodiments of the present disclosure.
  • FIG. 9 is a diagram illustrating values measured by a cell counter for isolated cells according to various embodiments of the present disclosure.
  • first and second may be used to describe various components, but the components are not limited by the terms. The terms are used only for the purpose of distinguishing one component from another.
  • first component may be referred to as the second component, and similarly, the second component may also be referred to as the first component.
  • the fat removal device and the cell separation device according to various embodiments of the present invention may be one or a combination of the above-described various devices.
  • the fat removing apparatus and the cell separation apparatus according to various embodiments of the present invention are not limited to the apparatus used for liposuction and cell separation.
  • the fat removal device and the cell separation device may be used in various dental treatments, orthopedic procedures, such as osteotomy and embryogenesis when used for medical purposes.
  • FIG. 1 is a view showing the configuration of a fat removal device 10 according to various embodiments of the present invention
  • Figure 2 is a view showing the operating state of the fat removal device 10 according to various embodiments of the present invention.
  • the fat removing device 10 includes a fat removing body 20, a hand piece 30, a tip 40, a temperature sensor 50, a peristaltic pump 60, and a cooling unit 70. ) And the controller 80.
  • the fat removal body 20 is electrically connected to the hand piece 30, which will be described later, and generates an ultrasonic electric signal and transmits it to the hand piece 30.
  • the hand piece 30 is electrically connected to the fat removing body portion 20 so as to receive ultrasonic electric signals from the fat removing body portion 20 to generate ultrasonic vibrations and to transmit the ultrasonic vibration to the tip portion 40 to be described later. It is.
  • the hand piece 30 is provided with an ultrasonic generator 30a (shown in FIG. 3) to generate the ultrasonic vibration.
  • the ultrasonic generator 30a (shown in FIG. 3) is made of a piezo electric so as to generate ultrasonic vibration in a piezoelectric manner.
  • the ultrasonic generator is described as an example of a piezoelectric device, but is not limited thereto. That is, the ultrasonic generator 30a (shown in FIG. 3) may also be applied to a device for generating ultrasonic waves in a manner other than piezoelectric.
  • the tip portion 40 is provided on the hand piece 30 to receive and vibrate the ultrasonic vibration.
  • the temperature sensor part 50 is provided in the tip part 40 to sense the rising temperature of the tip part 40 according to the ultrasonic vibration.
  • the peristaltic pump unit 60 is connected by the hand piece 30 and the cooling hose 90 and at the same time transfers the coolant A1 of the cooling unit 70 to the tip unit 40 to increase the temperature.
  • the fat removal main body 20 is provided to cool the tip 40.
  • the cooling unit 70 is connected by the peristaltic pump unit 60 and the cooling hose 90 and is configured to supply the cooling water A1.
  • the control unit 80 is configured to drive the peristaltic pump unit 60 at a preset temperature by receiving a signal sensed by the temperature sensor unit 50.
  • the predetermined temperature may be 80 or more. That is, the temperature sensor unit 50 detects the temperature of the tip unit 40, applies a monitored signal to the control unit 80, and the control unit 80 has a temperature of 80 or more of the tip unit 40.
  • the peristaltic pump part 60 is operated, and the peristaltic pump part 60 supplies the cooling water A1 to the hand piece 30.
  • the hand piece 30 may transfer the cooling water A1 to the tip part 40 to cool the temperature of the tip part 40, thereby preventing burns on the skin and muscle layers of the patient.
  • one end of the hand piece 30 is provided with a tip coupling portion 31 coupled to the tip portion 40, the tile end of the hand piece 30 to be connected to the peristaltic pump portion 60
  • the lock pump connection 32 is provided.
  • the tip coupling portion 31 is coupled to the mounting portion 41 formed on the tip portion 40 to receive the coolant A1 supplied to the hand piece 30.
  • the pump connection part 32 is connected to the peristaltic pump part 60 to supply the coolant A1 of the cooling part 70 into the hand piece 30.
  • the tip coupling part 31 and the pump connection part 32 are connected to each other, and at the same time, the coolant feed path 33 is able to transfer the coolant A1 to the tip part 40. ) Is provided.
  • one end of the tip portion 40 is provided with a mounting portion 41 to be mounted to the tip coupling portion 31, the tile end of the tip portion 40 so as to discharge the cooling water (A1) to the outside At least one discharge hole 42 is formed.
  • the tip part 40 receives and moves the coolant A1 supplied to the hand piece 30 and cools the temperature at the same time, and then cools the coolant A1 through the discharge holes 42. Discharge to the outside of the tip portion (40).
  • the coolant flow path 43 is provided to move the coolant A1 introduced through the hand piece 30 inside the tip part 40, the coolant introduced into the tip part 40 ( A1 is moved through the coolant movement path 43 and at the same time cools the temperature of the tip portion 40 and is discharged to the outside of the tip portion 40 through the discharge hole 42.
  • the cooling water A1 may be made of saline.
  • the cooling water A1 is described by way of example with saline, but is not limited thereto. That is, the cooling water A1 may be variously applied as long as the cooling water A1 is harmful to the human body.
  • the tip portion 40 is coupled to the hand piece 30.
  • the mounting portion 41 of the tip portion 40 is coupled to the tip coupling portion 31 provided at one end of the hand piece 30.
  • the cooling hose 90 is connected to the pump connection part 32 provided at the tile end of the hand feed, and the cooling hose 90 is connected to the peristaltic pump part 60.
  • the peristaltic pump unit 60 is connected to the cooling unit 70 by using another cooling hose 90.
  • the tip portion 40 is provided with a temperature sensor unit 50 for sensing the temperature of the tip portion 40, the temperature sensor unit 50 is electrically connected to the controller 80.
  • the fat removing body portion 20 is electrically connected to apply the ultrasonic signal of the fat removing body portion 20 to the hand piece 30.
  • FIG. 3 is a side cross-sectional view illustrating an operating state of the fat removing device 10 according to various embodiments of the present disclosure
  • FIG. 4 is an enlarged side cross-sectional view of portion A of FIG. 3.
  • the skin of a patient who wants to remove fat is slightly cut and then the tip part 40 provided in the hand piece 30 is inserted into the fat layer. Through the tip portion 40 is introduced a drug (not shown) to facilitate the removal of fat.
  • the drug consists of a drug diluted with normal cell line, anesthesia solution and anti-bleeding solution.
  • the damage of blood vessels and tissues is reduced, and the frictional resistance and skin resistance of the tip part 40 can be reduced and the procedure can be performed with less force.
  • a current is supplied to the fat removing body part 20, and the fat removing body part 20 generates an ultrasonic electric signal, and the ultrasonic electric signal is generated in the ultrasonic wave provided in the hand piece 30.
  • the ultrasonic generator 30a (shown in FIG. 3) generates ultrasonic vibration in a piezoelectric manner, and the ultrasonic vibration is vibrated back and forth while being transmitted to the tip portion 40. Due to the front and rear ultrasonic vibration of the tip portion 40, the fat cells of the fat layer are separated and destroyed by cavitation, mechanical breakdown, thermal effect, etc. due to bubble generation, so that fat is separated.
  • the fat thus separated is removed by the operation of a vacuum pump (not shown) provided in the fat removing body portion 20, and introduced into the tip portion 40 and provided in the fat removing body portion 20. Collected in a local storage container (not shown).
  • the tip portion 40 when the tip portion 40 is inserted into the skin of the patient and the ultrasonic vibrations, the tip portion 40 generates heat due to the front and rear ultrasonic vibrations.
  • the heat of the tip part 40 is detected by the temperature sensor part 50 provided in the tip part 40. If the tip unit 40 generates excessive heat due to ultrasonic vibration, as shown in FIG. 3, the temperature sensor unit 50 detects it and applies it to the controller 80.
  • the controller 80 drives the peristaltic pump unit 60 when the temperature exceeds a predetermined temperature. That is, the controller 80 drives the peristaltic pump unit 60 when the temperature of the tip unit 40 is measured to be about 80 or more.
  • the peristaltic pump unit 60 moves the cooling water A1 of the cooling unit 70 to supply the hand piece 30, and supplies the cooling water transfer path 33 provided inside the hand piece 30. It supplies to the tip portion 40 by using.
  • the tip portion 40 is provided with a coolant movement path 43 to move the coolant A1 introduced through the coolant transfer path 33 of the hand piece 30, the coolant A1. Is moved through the coolant movement path 43 of the tip portion 40 and simultaneously cools the elevated temperature of the tip portion 40.
  • the cooling water (A1) is discharged to the outside of the tip portion 40 through the discharge hole 42 of the tip portion (40).
  • the tip portion 40 thus cooled is collected by moving to a storage container (not shown) or a syringe (not shown) of the fat removing body portion 20 by removing the cooling water A1 and fat discharged to the patient's skin. Let's do it.
  • the conventional ultrasonic fat removal apparatus (not shown) has a disadvantage in that excessive heat is generated when the tip portion (not shown) vibrates before and after the vibration by ultrasonic vibration, thereby causing burns on the skin and muscle layers of the patient.
  • the present invention is provided with a temperature sensor unit 50 for sensing the temperature generated during the ultrasonic vibration of the tip portion 40 and the controller 80 for controlling the same to overcome this disadvantage, the control unit 80 is a tip portion ( By configuring to supply the coolant A1 that cools the elevated temperature of 40, the tip portion 40 can be prevented from burning the skin layer and the muscle layer of the patient due to being cooled by the coolant A1. Due to the fat removal procedure to prevent pain as well as to prevent the occurrence of swelling and pain alone due to cosmetic inflammation.
  • FIG. 5 is a side cross-sectional view illustrating a storage cap 100 of a cell separation apparatus according to various embodiments of the present disclosure
  • FIG. 6 is a storage cap 100 of a cell separation apparatus according to various embodiments of the present disclosure. It is a side sectional view which shows the engagement state with the tip part provided in the handpiece.
  • the cell separation device 110 the hand piece 30, the tip portion 40, the storage cap 100, the temperature sensor 50, the peristaltic pump 60, the cooling unit 70 and the control unit 80 It includes.
  • the hand piece 30 receives the ultrasonic electric signal generated from the main body (not shown) of the cell separation device 110 to generate the ultrasonic vibration and deliver the ultrasonic wave to the tip 40 which will be described later. It is electrically connected to a body portion (not shown) of the device 110.
  • the hand piece 30 is provided with an ultrasonic generator 30a to generate the ultrasonic vibration.
  • the ultrasonic generator 30a is formed of a piezo electric to generate ultrasonic vibration in a piezoelectric manner.
  • the ultrasonic generator 30a is described as an example of a piezoelectric device, but is not limited thereto. That is, the ultrasonic generator 30a may be applied to a device for generating ultrasonic waves in a manner other than piezoelectric.
  • the tip portion 40 is provided on the hand piece 30 to receive and vibrate the ultrasonic vibration.
  • the temperature sensor part 50 (shown in FIG. 2) is provided in the tip part 40 to sense the rising temperature of the tip part 40 according to the ultrasonic vibration.
  • the peristaltic pump unit 60 (shown in FIG. 2) is connected by the hand piece 30 and the cooling hose 90 and at the same time the coolant A1 of the cooling unit 70 (shown in FIG. 2) It is provided in the body portion (not shown) of the cell separation device 110 to cool the tip portion 40 is transferred to the tip portion 40 to increase the temperature.
  • the cooling unit 70 (shown in FIG. 2) is connected to the peristaltic pump unit 60 (shown in FIG. 2) by the cooling hose 90 and is configured to supply the cooling water A1.
  • the control unit 80 (shown in FIG. 2) is configured to drive the peristaltic pump unit 60 at a predetermined temperature by receiving a signal sensed by the temperature sensor unit 50.
  • the predetermined temperature may be 80 or more. That is, the temperature sensor unit 50 (shown in FIG. 2) senses the temperature of the tip unit 40, applies a monitored signal to the control unit 80, and the control unit 80 (shown in FIG. 2).
  • the peristaltic pump part 60 (shown in FIG. 2) is operated when the temperature of the tip part 40 is 80 or more, and the peristaltic pump part 60 supplies the cooling water A1 to the hand piece 30. Supply.
  • the hand piece 30 may cool the temperature of the tip part 40 by transferring the cooling water A1 to the tip part 40.
  • one end of the hand piece 30 is provided with a tip coupling portion 31 coupled to the tip portion 40, the tile end of the hand piece 30 is the peristaltic pump portion 60 (shown in Figure 2) Pump connection 32 is provided.
  • the tip coupling portion 31 is coupled to the mounting portion 41 formed on the tip portion 40 to receive the coolant A1 supplied to the hand piece 30.
  • the pump connection portion 32 is connected to the peristaltic pump portion 60 (shown in FIG. 2) to supply the coolant A1 of the cooling portion 70 (shown in FIG. 2) into the hand piece 30. .
  • the tip coupling part 31 and the pump connection part 32 are connected to each other, and at the same time, the coolant feed path 33 is able to transfer the coolant A1 to the tip part 40. ) Is provided.
  • one end of the tip portion 40 is provided with a mounting portion 41 to be mounted to the tip coupling portion 31, the tile end of the tip portion 40 so as to discharge the cooling water (A1) to the outside At least one discharge hole 42 is formed.
  • the tip part 40 receives and moves the coolant A1 supplied to the hand piece 30 and cools the temperature at the same time, and then cools the coolant A1 through the discharge holes 42. Discharge to the outside of the tip portion (40).
  • the coolant flow path 43 is provided to move the coolant A1 introduced through the hand piece 30 inside the tip part 40, the coolant introduced into the tip part 40 ( A1 is moved through the coolant movement path 43 and at the same time cools the temperature of the tip portion 40 and is discharged to the outside of the tip portion 40 through the discharge hole 42.
  • the cooling water A1 may be made of saline.
  • the cooling water A1 is described by way of example with saline, but is not limited thereto. That is, the cooling water A1 may be variously applied as long as the cooling water A1 is harmful to the human body.
  • the storage cap 100 may contain fat cells and cells, and in this state, the tip is inserted into the storage cap 100 at the same time as the handpiece is coupled to the storage cap 100 by the ultrasonic vibration of the ultrasonic generator. Fat cells and the cells can be dissolved and separated.
  • the storage cap 100 may include a cylindrical body portion 101, an inlet 102, an air passage 103, and an outlet 104.
  • the cylindrical body portion 101 may contain the fat cells and the cells.
  • the injection hole 102 may be provided on the side of the cylindrical body portion 101 so as to contain the adipocytes and the cells 25cc injected into the cylindrical body portion 101.
  • the air passage 103 may be provided on the side of the cylindrical body portion 101 to facilitate the injection and discharge of the fat cells and the cells into the cylindrical body portion 101 by introducing air.
  • the outlet 104 is provided in the lower portion of the cylindrical body portion 101 to discharge the separated cells to the outside.
  • Ultrasonic waves of the ultrasonic generator may be made of a wavelength of 18 KHz ⁇ 27 KHz.
  • the ultrasonic generator having a wavelength of 18 KHz to 27 KHz can separate human tissues into cells by using 34 KHz to 64 KHz while converting to buffer (boost) steps 1 to 10, as well as all cells except fat cells. Cells can be separated from the adipose tissue without damage. In other words, extracorporeal vascular fractions (SVF) can be isolated from adipose tissue without the use of conventional enzymes (collagens).
  • SVF extracorporeal vascular fractions
  • the present invention is to separate the human body tissues into cells, and to separate cells from adipose tissue, to separate cells except adipose cells from adipose tissue, and to extracellular matrix vascular fraction (SVF) in fat breakfast. It can separate the regenerative cells or stem cells or immature progenitor cells from adipose tissue.
  • SVF extracellular matrix vascular fraction
  • adipose tissue is composed of fat cells and extracellular vascular fractions which are systematically fused through collagen as a medium.
  • the cells and collagen connective tissue are separated by cell by using the existing enzyme (collagenase) to dissolve collagen that supports cell adhesion for 30 minutes at 36.5 degrees similar to body temperature. Therefore, the present invention can efficiently obtain extracellular matrix fractions from adipose tissue in minutes through the process of breaking down fat cells and collagen by means of ultrasound and breaking them down into cell units.
  • the tip portion 40 is coupled to the hand piece 30.
  • the mounting portion 41 of the tip portion 40 is coupled to the tip coupling portion 31 provided at one end of the hand piece 30.
  • the cooling hose 90 is connected to the pump connection part 32 provided at the tile end of the hand feed, and the cooling hose 90 is connected to the peristaltic pump part 60.
  • the peristaltic pump unit 60 is connected to the cooling unit 70 by using another cooling hose 90.
  • the tip portion 40 is provided with a temperature sensor unit 50 for sensing the temperature of the tip portion 40, the temperature sensor unit 50 is electrically connected to the controller 80.
  • the body portion (not shown) of the cell separation device is electrically connected to apply the ultrasonic signal to the hand piece 30.
  • Figure 7a is a view showing a state before inserting the tip portion 40 of the hand piece 30 in the storage cap 100 containing 25cc fat cells and cells according to various embodiments of the present invention
  • Figure 7b is a variety of the present invention
  • Figure 25 is a view showing a state of melting the cells after inserting the tip of the hand piece into the storage cap 100 containing 25cc fat cells and cells according to the embodiment
  • 8 is a view illustrating a state in which cells are separated by using a centrifugal separator in accordance with various embodiments of the present invention.
  • the adipocytes (G1) and the cells (G2) are injected into the injection hole 102 formed on the side surface of the cylindrical body portion 101 of the storage cap 100.
  • a current is supplied to a main body part (not shown) of the cell separation device 110, and the main body part generates an ultrasonic electric signal, and the ultrasonic electric signal is provided in the hand piece 30.
  • the ultrasonic generator 30a (shown in FIG. 6) generates ultrasonic vibration in a piezoelectric manner, and as shown in FIG. 7B, the ultrasonic vibration is transmitted to the tip portion 40 and vibrates back and forth. Due to the front and rear ultrasonic vibration of the tip portion 40, fat cells and cells dissolve.
  • the fat cells and cells of the storage cap 100 are broken down into cells by destroying fat cells and collagen through ultrasound.
  • the storage cap 100 dissolves cells in a matter of minutes through ultrasonic waves. Thereafter, the dissolved cells are separated into stem cells and other cells using a Winsim separator as shown in FIG. 8.
  • the cell G2 separated in the lower portion of the storage cap 100 is discharged to the outside through the discharge port 104 provided in the lower portion of the storage cap 100.
  • Cells thus extracted are put into a separation kit (not shown) to measure the number of cells. That is, the number of cells separated by the cell counter (not shown) is measured.
  • the cell counter measurement value is 589,000 live cells out of 590,000 total cells
  • the live count is measured as 99.6%. That is, the living cells are measured at 99.6%.
  • the cells and collagen connective tissues were separated into cells by reacting and dissolving collagen, which supports the cells to be attached by using existing enzymes (collagenase) for 30 minutes at a temperature similar to body temperature, for 30 minutes.
  • the present invention can efficiently obtain extracorporeal vascular fractions from adipose tissue in a matter of minutes through the process of breaking down fat cells, collagen and breaking down into cell units through ultrasound.

Abstract

L'invention concerne divers modes de réalisation associés à un appareil électronique pour retirer de la graisse en utilisant des ultrasons. Selon un mode de réalisation, l'appareil pour retirer de la graisse peut comprendre : un corps pour retirer de la graisse; une pièce à main ayant un générateur d'ultrasons relié électriquement au corps pour retirer de la graisse; une pointe qui est prévue sur la pièce à main; un capteur de température qui est prévu dans la pointe et détecte la température de la pointe; une pompe péristaltique qui est prévue dans le corps pour retirer de la graisse, et est reliée à la pièce à main et transporte de l'eau de refroidissement à la pointe; et un dispositif de commande qui reçoit un signal détecté par le capteur de température et entraîne la pompe péristaltique à une température prédéfinie. Divers autres modes de réalisation sont aussi possibles.
PCT/KR2016/002448 2015-03-26 2016-03-11 Appareil pour retirer de la graisse et appareil pour séparer des cellules WO2016153199A1 (fr)

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US15/714,073 US20180021599A1 (en) 2015-03-26 2017-09-25 Apparatus for removing fat and apparatus for separating cell
US17/970,833 US20230039796A1 (en) 2015-03-26 2022-10-21 Apparatus for removing fat and apparatus for separating cell

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20150042595 2015-03-26
KR10-2015-0042595 2015-03-26
KR1020150130446A KR20160115656A (ko) 2015-03-26 2015-09-15 지방 제거 장치 및 세포 분리 장치
KR10-2015-0130446 2015-09-15

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