WO2016153199A1

WO2016153199A1 - Apparatus for removing fat and apparatus for separating cell

Info

Publication number: WO2016153199A1
Application number: PCT/KR2016/002448
Authority: WO
Inventors: 김지환; 이성형
Original assignee: 김지환; 이성형
Priority date: 2015-03-26
Filing date: 2016-03-11
Publication date: 2016-09-29

Abstract

Disclosed are various embodiments related to an electronic apparatus for removing fat using ultrasound. According to an embodiment, the apparatus for removing fat may comprise: a body for removing fat; a handpiece having an ultrasound generator electrically connected to the body for removing fat; a tip which is provided on the handpiece; a temperature sensor which is provided in the tip and senses the temperature of the tip; a peristaltic pump which is provided in the body for removing fat, and is connected to the handpiece and transports cooling water to the tip; and a controller which receives a signal sensed by the temperature sensor and drives the peristaltic pump at a preset temperature. Various other embodiments are also possible.

Description

Fat removal device and cell separation device

Various embodiments of the present invention relate to a fat removal device for removing subcutaneous fat using ultrasound and a cell separation device for separating cells from fat cells.

In general, liposuction or liposuction is a plastic surgery to remove the fat layer accumulated excessively in the human body. In particular, the fat removal surgery can easily remove the fat of the area difficult to remove by exercise, diet and the like as needed. In other words, since exercise, dietary methods, etc., only reduce the size of fat cells, it is difficult to prevent thickening of the fat layer later, while liposuction reduces the number of fat cells.

These liposuctions are largely divided into mechanical devices and electronic devices, and mechanical devices are divided into dry plastic surgery and wet plastic surgery. First, a mechanical device was developed in the early 1980's, in which a cannula having a rough surface is inserted into a fat layer and then moved back and forth to separate and destroy fat cells to remove and remove fat.

However, this method suffers from a lot of pain and bleeding of the patient, the operator is also required a lot of labor and there is a problem that the correct procedure is difficult.

Wet plastic surgery has emerged as a method to solve this problem.

In the wet plastic surgery, a physiological saline solution containing a local anesthetic, a vasoconstrictive agent, etc. is infused into a desired fat layer to inflate the fat layer to several times its original thickness, and then, using the cannula, the fat is removed in the same manner as dry plastic surgery. That's the way it is.

Here, the cannula includes a tip.

The wet plastic surgery, because the thickness of the fat layer to be treated is inflated up to several times the original thickness, it is possible to increase the precision of the procedure, compared to dry plastic surgery, such as reducing the amount of bleeding during surgery by reducing capillaries by the vasoconstrictor It has many advantages. However, the wet plastic surgery, like dry plastic surgery, has to move the tip of the cannula on the rough surface back and forth to separate and destroy the fat cells, and thus cannot solve problems such as pain of the patient and excessive labor of the operator.

Therefore, to solve this problem, an electronic fat removal apparatus using ultrasonic waves has been developed. Such ultrasonic fat removal apparatus uses a device that converts ultrasonic electron energy into mechanical energy called vibration. First, the tip of the cannula, which generates ultrasonic vibration using piezoelectric crystals, which vibrates when an alternating current is provided, is inserted into the fat layer and vibrated. Bubbles are generated in the fat layer by ultrasonic vibration of the tip, and fat is separated by cavitation, micromechanical destruction, thermal effect, etc. by the generation of the bubbles, and by removing such fat Will be removed. At this time, the thermal effect serves to dissolve fat.

However, the conventional ultrasonic fat removal device has a disadvantage in that a cannula that dissolves fat using ultrasonic waves to remove subcutaneous fat generates a lot of heat at the tip portion of the cannula for thermal effects, causing burns to the skin and muscles of the user. Not only this. After the fat removal procedure, swelling occurred due to pain and burn inflammation of the patient.

As a result, there is a need for a cooling device to cool the heat generated at the tip of the cannula to overcome this disadvantage.

Therefore, in various embodiments of the present invention, the tip portion of the hand piece is configured to cool the heat generated during the vibration by ultrasonic waves, thereby preventing burns due to the heat generation of the tip on the skin layer and the muscle layer of the patient. The present invention provides a fat removing device that can prevent swelling in pain and cosmetic inflammation of a patient after the procedure.

In general, stem cell-related isolation methods that help treat cells by separating cells from human fat or other tissues are a rapidly growing field. In particular, the method of separating stem cells from human fat cells has previously used a method of dissolving cells and extracting byproducts by using enzymes (collagenase). However, unlike the acidic or alkaline solutions, enzymes (collagens) are not neutralized and have the same toxicity as the enzymes themselves. Unneutralized enzymes can dissolve other proteins and fats in the human body, and conventional stem cell separation equipment has a disadvantage in that it takes a lot of time to remove the remaining enzymes by washing these enzymes (collagenase) several times.

In other words, the cell separation method using an existing enzyme (collagenase) contains fat and cells collected from the human body in a container of a certain size and adds the enzyme (collagenase) to the temperature between 38 degrees and 39 degrees, similar to the human body. Dissolve cells for 40 minutes at. After that, the dissolved cells are centrifuged to separate stem cells and other cells.

Here, in order to neutralize the remaining enzyme (collagenase), it is washed with normal cell line several times to collect stem cells again.

As described above, the conventional cell separation method dissolves rotten fat cells and cells with enzymes (collagenase) for 40 minutes after the enzyme (collagenase) is neutralized with normal cells. At this time, the time required may take 20 minutes and 1 hour or more. This is the time that should be used because of the enzyme.

Therefore, in various embodiments of the present invention, by separating the cells by vibrating the tip of the hand piece by ultrasonic waves instead of the enzymes (collagenase), ultrasonic cells are designed to reduce the time to neutralize and neutralize the existing enzyme (collagenase). To provide a separation device.

According to various embodiments of the present disclosure, an electronic fat removing apparatus using ultrasonic waves may include: a fat removing body part; A hand piece having an ultrasonic generator electrically connected to the fat removal main body; A tip part provided on the hand piece; A temperature sensor unit provided at the tip unit to sense a temperature of the tip unit; A peristaltic pump part provided in the fat removing body part and connected to the hand piece and transferring coolant to the tip part; And a controller configured to receive the signal sensed by the temperature sensor unit and to drive the peristaltic pump unit at a preset temperature.

According to various embodiments of the present disclosure, an electronic fat removing apparatus using ultrasonic waves may include: a fat removing body part; A hand piece having an ultrasonic generator electrically connected to the fat removal main body; A tip part provided on the hand piece to receive and vibrate ultrasonic vibration of the ultrasonic generator; A temperature sensor unit provided at the tip unit to sense a rising temperature of the tip unit according to ultrasonic vibration; A peristaltic pump unit provided in the fat removing body unit and connected to the hand piece and transferring a coolant to the tip unit to cool the elevated temperature of the tip unit; A cooling unit connected to the peristaltic pump unit to supply the cooling water; And a controller configured to receive the signal sensed by the temperature sensor unit and to drive the peristaltic pump unit at a preset temperature.

According to various embodiments of the present disclosure, a cell separation device may include: a hand piece having an ultrasonic wave generator; A tip part provided on the hand piece; And a storage cap containing fat cells and cells, wherein the storage cap is coupled to the hand piece and inserts the tip into the storage cap to dissolve and separate the fat cells and the cells by ultrasonic waves of the ultrasonic generator. Can be.

According to various embodiments of the present invention,

By transmitting the ultrasonic vibration of the ultrasonic generator to the tip of the hand piece is configured to cool the heat generated during the vibration using a coolant to prevent the generation of heat of the tip inserted into the fat layer of the patient, thereby the skin layer and muscle layer of the patient It is possible to prevent burns on the skin, as well as to prevent pain of the patient after the procedure, and to prevent swelling caused by cosmetic inflammation.

That is, it is possible to remove subcutaneous fat quickly and efficiently without burns to the skin and muscle layers of the patient.

In addition, by separating the cells by ultrasonic waves without enzymes (collagenase) it can save a lot of time required to melt or neutralize the existing enzyme (collagenase) by only a few minutes. Therefore, it is possible to efficiently separate the cells from the fat cells.

1 is a view showing the configuration of a fat removal apparatus according to various embodiments of the present invention.

2 is a view illustrating an operating state of a fat removing device according to various embodiments of the present disclosure.

Figure 3 is a side cross-sectional view showing an operating state of the fat removal device according to various embodiments of the present invention.

4 is an enlarged side cross-sectional view of portion A of FIG.

Figure 5 is a side cross-sectional view showing a storage cap of the cell separation apparatus according to various embodiments of the present invention.

Figure 6 is a side cross-sectional view showing a coupling state of the tip portion provided in the storage cap and the hand piece of the cell separation apparatus according to various embodiments of the present invention.

Figure 7a is a view showing a state before inserting the tip of the hand piece in a storage cap containing 25cc fat cells and cells according to various embodiments of the present invention.

7B is a view illustrating a process of melting a cell after inserting a tip of a hand piece into a storage cap containing 25 cc of fat cells and cells according to various embodiments of the present disclosure.

8 is a view showing a state in which cells are separated using a centrifuge for dissolved cells according to various embodiments of the present disclosure.

9 is a diagram illustrating values measured by a cell counter for isolated cells according to various embodiments of the present disclosure.

Terms used in various embodiments of the present invention will be briefly described, and various embodiments of the present invention will be described in detail.

The terminology used in the various embodiments of the present invention selected general terms widely used as possible in consideration of the functions in the various embodiments of the present invention, but this is the intention or precedent of the person skilled in the art, the emergence of new technology And so on. In addition, in certain cases, there is a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of various embodiments of the present invention. Therefore, terms used in various embodiments of the present disclosure should be defined based on the meanings of the terms and the contents throughout the various embodiments of the present disclosure, rather than merely names of the terms.

In addition, terms including ordinal numbers such as first and second may be used to describe various components, but the components are not limited by the terms. The terms are used only for the purpose of distinguishing one component from another. For example, without departing from the scope of the present invention, the first component may be referred to as the second component, and similarly, the second component may also be referred to as the first component.

The fat removal device and the cell separation device according to various embodiments of the present invention may be one or a combination of the above-described various devices. In addition, it will be apparent to those skilled in the art that the fat removing apparatus and the cell separation apparatus according to various embodiments of the present invention are not limited to the apparatus used for liposuction and cell separation. For example, the fat removal device and the cell separation device may be used in various dental treatments, orthopedic procedures, such as osteotomy and embryogenesis when used for medical purposes.

1 is a view showing the configuration of a fat removal device 10 according to various embodiments of the present invention, Figure 2 is a view showing the operating state of the fat removal device 10 according to various embodiments of the present invention.

1 and 2, the configuration of the electronic fat removal apparatus 10 using ultrasonic waves will be described. The fat removing device 10 includes a fat removing body 20, a hand piece 30, a tip 40, a temperature sensor 50, a peristaltic pump 60, and a cooling unit 70. ) And the controller 80.

The fat removal body 20 is electrically connected to the hand piece 30, which will be described later, and generates an ultrasonic electric signal and transmits it to the hand piece 30.

The hand piece 30 is electrically connected to the fat removing body portion 20 so as to receive ultrasonic electric signals from the fat removing body portion 20 to generate ultrasonic vibrations and to transmit the ultrasonic vibration to the tip portion 40 to be described later. It is.

The hand piece 30 is provided with an ultrasonic generator 30a (shown in FIG. 3) to generate the ultrasonic vibration.

The ultrasonic generator 30a (shown in FIG. 3) is made of a piezo electric so as to generate ultrasonic vibration in a piezoelectric manner.

In the present embodiment, the ultrasonic generator is described as an example of a piezoelectric device, but is not limited thereto. That is, the ultrasonic generator 30a (shown in FIG. 3) may also be applied to a device for generating ultrasonic waves in a manner other than piezoelectric.

The tip portion 40 is provided on the hand piece 30 to receive and vibrate the ultrasonic vibration.

The temperature sensor part 50 is provided in the tip part 40 to sense the rising temperature of the tip part 40 according to the ultrasonic vibration.

The peristaltic pump unit 60 is connected by the hand piece 30 and the cooling hose 90 and at the same time transfers the coolant A1 of the cooling unit 70 to the tip unit 40 to increase the temperature. The fat removal main body 20 is provided to cool the tip 40.

The cooling unit 70 is connected by the peristaltic pump unit 60 and the cooling hose 90 and is configured to supply the cooling water A1.

The control unit 80 is configured to drive the peristaltic pump unit 60 at a preset temperature by receiving a signal sensed by the temperature sensor unit 50.

The predetermined temperature may be 80 or more. That is, the temperature sensor unit 50 detects the temperature of the tip unit 40, applies a monitored signal to the control unit 80, and the control unit 80 has a temperature of 80 or more of the tip unit 40. The peristaltic pump part 60 is operated, and the peristaltic pump part 60 supplies the cooling water A1 to the hand piece 30. The hand piece 30 may transfer the cooling water A1 to the tip part 40 to cool the temperature of the tip part 40, thereby preventing burns on the skin and muscle layers of the patient.

In addition, one end of the hand piece 30 is provided with a tip coupling portion 31 coupled to the tip portion 40, the tile end of the hand piece 30 to be connected to the peristaltic pump portion 60 The lock pump connection 32 is provided.

That is, the tip coupling portion 31 is coupled to the mounting portion 41 formed on the tip portion 40 to receive the coolant A1 supplied to the hand piece 30. The pump connection part 32 is connected to the peristaltic pump part 60 to supply the coolant A1 of the cooling part 70 into the hand piece 30.

Inside the hand piece 30, the tip coupling part 31 and the pump connection part 32 are connected to each other, and at the same time, the coolant feed path 33 is able to transfer the coolant A1 to the tip part 40. ) Is provided.

In addition, one end of the tip portion 40 is provided with a mounting portion 41 to be mounted to the tip coupling portion 31, the tile end of the tip portion 40 so as to discharge the cooling water (A1) to the outside At least one discharge hole 42 is formed.

That is, the tip part 40 receives and moves the coolant A1 supplied to the hand piece 30 and cools the temperature at the same time, and then cools the coolant A1 through the discharge holes 42. Discharge to the outside of the tip portion (40).

Since the coolant flow path 43 is provided to move the coolant A1 introduced through the hand piece 30 inside the tip part 40, the coolant introduced into the tip part 40 ( A1 is moved through the coolant movement path 43 and at the same time cools the temperature of the tip portion 40 and is discharged to the outside of the tip portion 40 through the discharge hole 42.

The cooling water A1 may be made of saline. In the present embodiment, the cooling water A1 is described by way of example with saline, but is not limited thereto. That is, the cooling water A1 may be variously applied as long as the cooling water A1 is harmful to the human body.

In this state, the assembly process of the fat removal device 10 will be described in more detail. First, as shown in FIGS. 1 and 2, the tip portion 40 is coupled to the hand piece 30. The mounting portion 41 of the tip portion 40 is coupled to the tip coupling portion 31 provided at one end of the hand piece 30. The cooling hose 90 is connected to the pump connection part 32 provided at the tile end of the hand feed, and the cooling hose 90 is connected to the peristaltic pump part 60. The peristaltic pump unit 60 is connected to the cooling unit 70 by using another cooling hose 90.

At this time, the tip portion 40 is provided with a temperature sensor unit 50 for sensing the temperature of the tip portion 40, the temperature sensor unit 50 is electrically connected to the controller 80. The fat removing body portion 20 is electrically connected to apply the ultrasonic signal of the fat removing body portion 20 to the hand piece 30.

In this state, the operation process of the fat removal device 10 will be described in more detail. 3 is a side cross-sectional view illustrating an operating state of the fat removing device 10 according to various embodiments of the present disclosure, and FIG. 4 is an enlarged side cross-sectional view of portion A of FIG. 3.

First, as described above with reference to FIGS. 1 and 2, the skin of a patient who wants to remove fat is slightly cut and then the tip part 40 provided in the hand piece 30 is inserted into the fat layer. Through the tip portion 40 is introduced a drug (not shown) to facilitate the removal of fat. The drug consists of a drug diluted with normal cell line, anesthesia solution and anti-bleeding solution.

That is, in the present embodiment, when the drug is put into the fat of the patient, the damage of blood vessels and tissues is reduced, and the frictional resistance and skin resistance of the tip part 40 can be reduced and the procedure can be performed with less force.

In this state, a current is supplied to the fat removing body part 20, and the fat removing body part 20 generates an ultrasonic electric signal, and the ultrasonic electric signal is generated in the ultrasonic wave provided in the hand piece 30. To portion 30a (shown in FIG. 3). The ultrasonic generator 30a (shown in FIG. 3) generates ultrasonic vibration in a piezoelectric manner, and the ultrasonic vibration is vibrated back and forth while being transmitted to the tip portion 40. Due to the front and rear ultrasonic vibration of the tip portion 40, the fat cells of the fat layer are separated and destroyed by cavitation, mechanical breakdown, thermal effect, etc. due to bubble generation, so that fat is separated. The fat thus separated is removed by the operation of a vacuum pump (not shown) provided in the fat removing body portion 20, and introduced into the tip portion 40 and provided in the fat removing body portion 20. Collected in a local storage container (not shown).

In this case, when the tip portion 40 is inserted into the skin of the patient and the ultrasonic vibrations, the tip portion 40 generates heat due to the front and rear ultrasonic vibrations. The heat of the tip part 40 is detected by the temperature sensor part 50 provided in the tip part 40. If the tip unit 40 generates excessive heat due to ultrasonic vibration, as shown in FIG. 3, the temperature sensor unit 50 detects it and applies it to the controller 80. The controller 80 drives the peristaltic pump unit 60 when the temperature exceeds a predetermined temperature. That is, the controller 80 drives the peristaltic pump unit 60 when the temperature of the tip unit 40 is measured to be about 80 or more. The peristaltic pump unit 60 moves the cooling water A1 of the cooling unit 70 to supply the hand piece 30, and supplies the cooling water transfer path 33 provided inside the hand piece 30. It supplies to the tip portion 40 by using.

Since the tip portion 40 is provided with a coolant movement path 43 to move the coolant A1 introduced through the coolant transfer path 33 of the hand piece 30, the coolant A1. Is moved through the coolant movement path 43 of the tip portion 40 and simultaneously cools the elevated temperature of the tip portion 40.

And, as shown in Figure 4, the cooling water (A1) is discharged to the outside of the tip portion 40 through the discharge hole 42 of the tip portion (40).

The tip portion 40 thus cooled is collected by moving to a storage container (not shown) or a syringe (not shown) of the fat removing body portion 20 by removing the cooling water A1 and fat discharged to the patient's skin. Let's do it.

As such, the conventional ultrasonic fat removal apparatus (not shown) has a disadvantage in that excessive heat is generated when the tip portion (not shown) vibrates before and after the vibration by ultrasonic vibration, thereby causing burns on the skin and muscle layers of the patient.

Therefore, the present invention is provided with a temperature sensor unit 50 for sensing the temperature generated during the ultrasonic vibration of the tip portion 40 and the controller 80 for controlling the same to overcome this disadvantage, the control unit 80 is a tip portion ( By configuring to supply the coolant A1 that cools the elevated temperature of 40, the tip portion 40 can be prevented from burning the skin layer and the muscle layer of the patient due to being cooled by the coolant A1. Due to the fat removal procedure to prevent pain as well as to prevent the occurrence of swelling and pain alone due to cosmetic inflammation.

5 is a side cross-sectional view illustrating a storage cap 100 of a cell separation apparatus according to various embodiments of the present disclosure, and FIG. 6 is a storage cap 100 of a cell separation apparatus according to various embodiments of the present disclosure. It is a side sectional view which shows the engagement state with the tip part provided in the handpiece.

5 and 6, the configuration of the electronic cell separation device 110 using ultrasonic waves will be described. The cell separation device 110, the hand piece 30, the tip portion 40, the storage cap 100, the temperature sensor 50, the peristaltic pump 60, the cooling unit 70 and the control unit 80 It includes.

The hand piece 30 receives the ultrasonic electric signal generated from the main body (not shown) of the cell separation device 110 to generate the ultrasonic vibration and deliver the ultrasonic wave to the tip 40 which will be described later. It is electrically connected to a body portion (not shown) of the device 110.

The hand piece 30 is provided with an ultrasonic generator 30a to generate the ultrasonic vibration.

The ultrasonic generator 30a is formed of a piezo electric to generate ultrasonic vibration in a piezoelectric manner.

In the present embodiment, the ultrasonic generator 30a is described as an example of a piezoelectric device, but is not limited thereto. That is, the ultrasonic generator 30a may be applied to a device for generating ultrasonic waves in a manner other than piezoelectric.

The temperature sensor part 50 (shown in FIG. 2) is provided in the tip part 40 to sense the rising temperature of the tip part 40 according to the ultrasonic vibration.

The peristaltic pump unit 60 (shown in FIG. 2) is connected by the hand piece 30 and the cooling hose 90 and at the same time the coolant A1 of the cooling unit 70 (shown in FIG. 2) It is provided in the body portion (not shown) of the cell separation device 110 to cool the tip portion 40 is transferred to the tip portion 40 to increase the temperature.

The cooling unit 70 (shown in FIG. 2) is connected to the peristaltic pump unit 60 (shown in FIG. 2) by the cooling hose 90 and is configured to supply the cooling water A1.

The control unit 80 (shown in FIG. 2) is configured to drive the peristaltic pump unit 60 at a predetermined temperature by receiving a signal sensed by the temperature sensor unit 50.

The predetermined temperature may be 80 or more. That is, the temperature sensor unit 50 (shown in FIG. 2) senses the temperature of the tip unit 40, applies a monitored signal to the control unit 80, and the control unit 80 (shown in FIG. 2). The peristaltic pump part 60 (shown in FIG. 2) is operated when the temperature of the tip part 40 is 80 or more, and the peristaltic pump part 60 supplies the cooling water A1 to the hand piece 30. Supply. The hand piece 30 may cool the temperature of the tip part 40 by transferring the cooling water A1 to the tip part 40.

In addition, one end of the hand piece 30 is provided with a tip coupling portion 31 coupled to the tip portion 40, the tile end of the hand piece 30 is the peristaltic pump portion 60 (shown in Figure 2) Pump connection 32 is provided.

That is, the tip coupling portion 31 is coupled to the mounting portion 41 formed on the tip portion 40 to receive the coolant A1 supplied to the hand piece 30. The pump connection portion 32 is connected to the peristaltic pump portion 60 (shown in FIG. 2) to supply the coolant A1 of the cooling portion 70 (shown in FIG. 2) into the hand piece 30. .

In addition, the storage cap 100 may contain fat cells and cells, and in this state, the tip is inserted into the storage cap 100 at the same time as the handpiece is coupled to the storage cap 100 by the ultrasonic vibration of the ultrasonic generator. Fat cells and the cells can be dissolved and separated.

As described above with reference to FIG. 5, the storage cap 100 may include a cylindrical body portion 101, an inlet 102, an air passage 103, and an outlet 104.

The cylindrical body portion 101 may contain the fat cells and the cells.

The injection hole 102 may be provided on the side of the cylindrical body portion 101 so as to contain the adipocytes and the cells 25cc injected into the cylindrical body portion 101.

The air passage 103 may be provided on the side of the cylindrical body portion 101 to facilitate the injection and discharge of the fat cells and the cells into the cylindrical body portion 101 by introducing air.

The outlet 104 is provided in the lower portion of the cylindrical body portion 101 to discharge the separated cells to the outside.

Ultrasonic waves of the ultrasonic generator may be made of a wavelength of 18 KHz ~ 27 KHz. The ultrasonic generator having a wavelength of 18 KHz to 27 KHz can separate human tissues into cells by using 34 KHz to 64 KHz while converting to buffer (boost) steps 1 to 10, as well as all cells except fat cells. Cells can be separated from the adipose tissue without damage. In other words, extracorporeal vascular fractions (SVF) can be isolated from adipose tissue without the use of conventional enzymes (collagens).

The present invention is to separate the human body tissues into cells, and to separate cells from adipose tissue, to separate cells except adipose cells from adipose tissue, and to extracellular matrix vascular fraction (SVF) in fat breakfast. It can separate the regenerative cells or stem cells or immature progenitor cells from adipose tissue.

More specifically, adipose tissue is composed of fat cells and extracellular vascular fractions which are systematically fused through collagen as a medium. The cells and collagen connective tissue are separated by cell by using the existing enzyme (collagenase) to dissolve collagen that supports cell adhesion for 30 minutes at 36.5 degrees similar to body temperature. Therefore, the present invention can efficiently obtain extracellular matrix fractions from adipose tissue in minutes through the process of breaking down fat cells and collagen by means of ultrasound and breaking them down into cell units.

In this state, the assembly process of the cell separation device 110 will be described in more detail. First, as shown in FIG. 6, the tip portion 40 is coupled to the hand piece 30. The mounting portion 41 of the tip portion 40 is coupled to the tip coupling portion 31 provided at one end of the hand piece 30. The cooling hose 90 is connected to the pump connection part 32 provided at the tile end of the hand feed, and the cooling hose 90 is connected to the peristaltic pump part 60. The peristaltic pump unit 60 is connected to the cooling unit 70 by using another cooling hose 90.

At this time, the tip portion 40 is provided with a temperature sensor unit 50 for sensing the temperature of the tip portion 40, the temperature sensor unit 50 is electrically connected to the controller 80. The body portion (not shown) of the cell separation device is electrically connected to apply the ultrasonic signal to the hand piece 30.

Figure 7a is a view showing a state before inserting the tip portion 40 of the hand piece 30 in the storage cap 100 containing 25cc fat cells and cells according to various embodiments of the present invention, Figure 7b is a variety of the present invention Figure 25 is a view showing a state of melting the cells after inserting the tip of the hand piece into the storage cap 100 containing 25cc fat cells and cells according to the embodiment. 8 is a view illustrating a state in which cells are separated by using a centrifugal separator in accordance with various embodiments of the present invention.

As shown in FIG. 7A, the adipocytes (G1) and the cells (G2) are injected into the injection hole 102 formed on the side surface of the cylindrical body portion 101 of the storage cap 100.

In this state, a current is supplied to a main body part (not shown) of the cell separation device 110, and the main body part generates an ultrasonic electric signal, and the ultrasonic electric signal is provided in the hand piece 30. To the generator 30a (shown in FIG. 6). The ultrasonic generator 30a (shown in FIG. 6) generates ultrasonic vibration in a piezoelectric manner, and as shown in FIG. 7B, the ultrasonic vibration is transmitted to the tip portion 40 and vibrates back and forth. Due to the front and rear ultrasonic vibration of the tip portion 40, fat cells and cells dissolve.

That is, the fat cells and cells of the storage cap 100 are broken down into cells by destroying fat cells and collagen through ultrasound. In other words, the storage cap 100 dissolves cells in a matter of minutes through ultrasonic waves. Thereafter, the dissolved cells are separated into stem cells and other cells using a Winsim separator as shown in FIG. 8.

And, the cell G2 separated in the lower portion of the storage cap 100 is discharged to the outside through the discharge port 104 provided in the lower portion of the storage cap 100. Cells thus extracted are put into a separation kit (not shown) to measure the number of cells. That is, the number of cells separated by the cell counter (not shown) is measured.

As shown in FIG. 9, since the cell counter measurement value is 589,000 live cells out of 590,000 total cells, the live count is measured as 99.6%. That is, the living cells are measured at 99.6%.

In other words, the cells and collagen connective tissues were separated into cells by reacting and dissolving collagen, which supports the cells to be attached by using existing enzymes (collagenase) for 30 minutes at a temperature similar to body temperature, for 30 minutes. The present invention can efficiently obtain extracorporeal vascular fractions from adipose tissue in a matter of minutes through the process of breaking down fat cells, collagen and breaking down into cell units through ultrasound.

The fat removal device and the cell separation device according to various embodiments of the present invention described above are not limited to the above-described embodiments and drawings, and various substitutions, modifications, and changes are possible within the technical scope of the present invention. It will be apparent to those of ordinary skill in the art.

Claims

In the fat removal device,

Fat removal body portion;

A hand piece having an ultrasonic generator electrically connected to the fat removal main body;

A tip part provided on the hand piece;

A temperature sensor unit provided at the tip unit to sense a temperature of the tip unit;

A peristaltic pump part provided in the fat removing body part and connected to the hand piece and transferring coolant to the tip part; And

And a controller configured to receive the signal sensed by the temperature sensor unit and to drive the peristaltic pump unit at a predetermined temperature.
In the fat removal device,

Fat removal body portion;

A hand piece having an ultrasonic generator electrically connected to the fat removal main body;

A tip part provided on the hand piece to receive and vibrate ultrasonic vibration of the ultrasonic generator;

A temperature sensor unit provided at the tip unit to sense a rising temperature of the tip unit according to ultrasonic vibration;

A peristaltic pump unit provided in the fat removing body unit and connected to the hand piece and transferring a coolant to the tip unit to cool the elevated temperature of the tip unit;

A cooling unit connected to the peristaltic pump unit to supply the cooling water; And

And a controller configured to receive the signal sensed by the temperature sensor unit and to drive the pump unit at a predetermined temperature.
3. The fat removing device of claim 2, wherein one end of the hand piece is provided with a tip coupling part engaged with the tip part, and a tile end is provided with a pump connection part connected with the peristaltic pump part.
3. The fat removal apparatus of claim 2, wherein a cooling water transfer path for connecting the tip coupling part and the pump connection part to each other and transferring the cooling water is provided inside the hand piece.
5. The fat removal apparatus of claim 4, wherein one end of the tip portion includes a mounting portion mounted to the tip coupling portion, and the tile end forms at least one discharge hole for discharging the cooling water to the outside of the tip portion.
The fat removal apparatus of claim 2, wherein a cooling water moving path for moving the cooling water introduced through the hand piece is provided in the tip part.
The fat removal apparatus of Claim 2 in which the said cooling water consists of a saline solution.
The fat removing device of claim 2, wherein the predetermined temperature is 80 or more.
In the cell separation device,

A hand piece having an ultrasonic generator;

A tip part provided on the hand piece; And

A storage cap containing fat cells and cells,

The storage cap is coupled to the hand piece and the cell separation device to insert the tip into the storage cap to dissolve and separate the fat cells and the cells by the ultrasonic wave of the ultrasonic generator.
10. The apparatus of claim 9, wherein the storage cap comprises: a cylindrical body portion including an opening coupled to the hand piece at an upper end thereof;

An injection hole provided at a side of the cylindrical body portion to inject the fat cells and the cells into the cylindrical body portion;

An air passage provided at a side of the cylindrical body portion; And

Cell separation device comprising a discharge port provided in the lower portion of the cylindrical body portion for discharging the separated cells.
The cell separation apparatus of claim 9, wherein the ultrasonic wave of the ultrasonic wave generation unit has a wavelength of 18 KHz to 27 KHz.