WO2016149876A1 - 多极微动能饮用水在制备用于减肥的饮料、保健品或药物中的用途 - Google Patents

多极微动能饮用水在制备用于减肥的饮料、保健品或药物中的用途 Download PDF

Info

Publication number
WO2016149876A1
WO2016149876A1 PCT/CN2015/074748 CN2015074748W WO2016149876A1 WO 2016149876 A1 WO2016149876 A1 WO 2016149876A1 CN 2015074748 W CN2015074748 W CN 2015074748W WO 2016149876 A1 WO2016149876 A1 WO 2016149876A1
Authority
WO
WIPO (PCT)
Prior art keywords
frequency electromagnetic
electromagnetic wave
water
drinking water
kinetic energy
Prior art date
Application number
PCT/CN2015/074748
Other languages
English (en)
French (fr)
Inventor
王卫星
Original Assignee
欢乐海（北京）水业科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 欢乐海（北京）水业科技有限公司 filed Critical 欢乐海（北京）水业科技有限公司
Priority to PCT/CN2015/074748 priority Critical patent/WO2016149876A1/zh
Priority to AU2015387916A priority patent/AU2015387916A1/en
Publication of WO2016149876A1 publication Critical patent/WO2016149876A1/zh

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38—Other non-alcoholic beverages
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/30—Treatment of water, waste water, or sewage by irradiation

Definitions

  • the invention belongs to the technical field of water treatment, and particularly relates to the use of multi-pole micro-kinetic energy drinking water in preparing beverages, health care products or medicines for weight loss.
  • Water is an inorganic substance composed of two elements of hydrogen and oxygen, and is a colorless and odorless transparent liquid under normal temperature and normal pressure. Water is one of the most common substances, an important resource for all life, including human beings, and the most important component of living things. Water plays an important role in the evolution of life. Humans have long begun to understand water, and water is regarded as a basic component in the ancient and simple material view of the East and West.
  • the water molecule itself has its special structural shape, it is composed of two hydrogen and one oxygen ion combined at an angle of 104.5. It is an ionization system with an electronic "magnetic polarity". Water molecules exhibit solid crystals below zero and below zero, which we call ice. However, water is not completely liquid at a temperature of from zero to 60 ° C, but exists in the form of a liquid crystal. However, if the temperature is higher than 60 ° C, the liquid crystal molecules will be destroyed.
  • the form of the diluted solute molecules can be contained in its liquid crystals, which is a generally understood dissolution process.
  • the new discovery is that when a solute molecule is separated from a water molecule by some means (eg, through multiple dilutions and oscillations), the water molecule can still maintain a "shape" memory of the solute molecule. When the body cells are exposed to such water molecules, the "shape" memory of the solute molecule is treated as a true solute molecule, which is the cornerstone of homeopathic therapy with more than 200 years of history.
  • Water is composed of two elements of hydrogen and oxygen, but does not exist in nature as a single water molecule. Instead, a group of water molecules (H 2 O) n is present in nature (ie, the above liquid state). Crystallization), the group of water molecules may be chain, ring, agglomerate or grape-like, with at least five water molecules, and more than a dozen, tens to hundreds.
  • Electromagnetic waves have been used to treat water molecules to increase the energy level of water molecules or to disinfect or prevent and remove dirt.
  • these treatment methods do not control the effect of the treatment well, and the effect of the treated water is mostly limited to drinking, and there is no other additional advantageous effect.
  • the present invention provides a multi-pole micro-kinetic energy drinking water obtained by electromagnetic wave non-contact treatment, a preparation method thereof and use thereof.
  • the present invention provides a multi-pole micro-kinetic energy drinking water prepared by non-contact treatment of drinking water raw water by electromagnetic waves, and the ultraviolet absorption peak is short-waved compared with the raw water of drinking water.
  • the direction shift is 25 nm to 40 nm, preferably 28 nm to 30 nm in the short-wave direction, and most preferably 30 nm or 28 nm in the short-wave direction.
  • the spectral intensity of the fluorescent radiation of the multi-pole micro-kinetic energy drinking water of the invention is greatly improved in the spectrum of 300 nm to 400 nm, and repeated experiments on the same sample show that the experiment has good repeatability. .
  • the drinking water raw water is ordinary tap water or various kinds of mineral water or pure water, such as Evian natural mineral water, Cambodia glacier mineral water, Nongfu Spring, Wahaha, and the like.
  • the multi-pole micro-kinetic energy drinking water of the present invention is best consumed or used within 72 hours after preparation.
  • the present invention provides a method for preparing the above-mentioned multi-pole micro-kinetic energy drinking water, which comprises non-contact treatment of drinking water raw water by using electromagnetic waves of low, medium and high frequencies, wherein the frequency of low-frequency electromagnetic waves
  • the range is 30-100 kHz
  • the frequency range of the intermediate frequency electromagnetic wave is 550-720 kHz
  • the frequency range of the high frequency electromagnetic wave is 300-725 MHz.
  • the drinking water raw water is ordinary tap water or various kinds of mineral water or pure water, such as Evian natural mineral water, Cambodia glacier mineral water, Nongfu Spring, Wahaha, and the like.
  • the low frequency electromagnetic wave has a frequency in the range of 45-75 kHz.
  • the intermediate frequency electromagnetic wave has a frequency range of 600-720 kHz.
  • the high frequency electromagnetic wave has a frequency in the range of 300-425 MHz.
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are a sine wave, a square wave, a sharp wave, a sawtooth wave or a trapezoidal wave.
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave waveform may be the same or different, preferably the same.
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave propagate in the same direction.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • the low-frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed by the electromagnetic wave non-contact treatment of the drinking water raw water treatment by using three different frequencies of low, medium and high frequencies, and then the intermediate frequency electromagnetic wave and the high frequency electromagnetic wave are simultaneously processed.
  • the time for simultaneous treatment using the low frequency electromagnetic wave and the high frequency electromagnetic wave is 10-30 minutes, preferably 10-20 minutes; the time for simultaneous treatment using the intermediate frequency electromagnetic wave and the high frequency electromagnetic wave is 10-30 minutes, preferably 10-20 minutes. .
  • the prepared ultraviolet light absorption peak of the multi-pole micro-kinetic energy drinking water is shifted to the short-wave direction by 25 nm to 40 nm, preferably to the short-wave direction by 28 nm to 30 nm, and most preferably to the short-wave direction. 30 nm or 28 nm.
  • the spectral intensity of the fluorescent radiation of the multi-pole micro-kinetic energy drinking water of the invention is greatly improved in the spectrum of 300 nm to 400 nm, and repeated experiments on the same sample show that the experiment has good repeatability. .
  • a method and apparatus for generating electromagnetic waves are conventional techniques in the art.
  • the present invention provides the use of the above-described multi-pole micro-kinetic energy drinking water in the preparation of various mineral water, purified water, beverages, health care products or medicines.
  • the beverage is a functional beverage and can be used for anti-fatigue, weight loss, laxative, blood pressure lowering, blood sugar lowering, blood uric acid and blood urea.
  • the health care product or drug can be used for anti-fatigue, weight loss, laxative, blood pressure lowering, blood sugar lowering, blood uric acid lowering and blood urea lowering.
  • the invention adopts electromagnetic wave non-contact type treatment water, without adding any additives, is simple to manufacture, has high production efficiency and low cost.
  • the multi-pole micro-kinetic energy drinking water of the present invention is more than the drinking water before treatment.
  • the original water produced a significant shift in the ultraviolet absorption peak to the short-wave direction.
  • the spectral intensity of the fluorescent radiation was greatly improved in the 300-400 nm spectrum, and more extranuclear electrons were in the high-energy non-radiative energy level.
  • Animal experiments have proven to have anti-fatigue, weight loss, laxative, blood pressure lowering, blood sugar lowering, blood uric acid and blood urea.
  • FIG 1 shows the effect of water in different treatment groups on peroxide-induced DNA damage
  • Figure 2 shows the effect of medium in different treatment groups on cells
  • 3 is an absorption spectrum distribution of the ordinary drinking water raw water and the multi-pole micro kinetic energy of the present invention obtained by using the ordinary drinking water raw water;
  • Figure 6 is a fluorescence steady state transient test result of ordinary drinking water (raw water) and its multi-pole micro-kinetic energy drinking water;
  • Figure 7 is a fluorescence steady state transient test result of Evian natural mineral water (raw water) and multi-pole micro-kinetic energy drinking water made therewith;
  • Figure 8 shows the fluorescence steady-state transient test results of Vietnamese glacial mineral water (raw water) and multi-pole micro-kinetic energy drinking water made therewith;
  • Figure 9 shows the results of repeatability test of three samples of multi-pole micro-kinetic energy drinking water made from raw water of Georgia glacial mineral water;
  • Figure 10 is a graph showing the effect of multi-polar micro-kinetic energy drinking water on the food intake of an obese model rat
  • Figure 11 is a graph showing the effect of multi-polar micro-kinetic energy drinking water on the body weight of rats in obesity prevention model
  • Figure 13 is a graph showing the effect of multi-polar micro-kinetic energy drinking water on the ratio of fat to body fat in the kidney and testis of an obese model rat.
  • Embodiment 1 Method for preparing multi-pole micro-kinetic energy drinking water of the present invention
  • electromagnetic waves of three different frequencies of low, medium and high are applied in a non-contact manner, wherein the frequency of the low frequency electromagnetic wave is 30 kHz, the frequency range of the intermediate frequency electromagnetic wave is 550 kHz, and the frequency range of the high frequency electromagnetic wave is 300 MHz. .
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are both sinusoidal waves, and the electromagnetic wave propagation directions of the two are the same.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • non-contact electromagnetic waves of low, medium and high frequencies are applied, wherein the frequency of the low frequency electromagnetic waves is 100 kHz, the frequency range of the intermediate frequency electromagnetic waves is 720 kHz, and the frequency range of the high frequency electromagnetic waves is 725 MHz. .
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are both square waves, and the electromagnetic wave propagation directions of the two are the same.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • electromagnetic waves of low, medium and high frequencies are applied non-contactly, wherein the frequency of the low frequency electromagnetic waves is 45 kHz, the frequency range of the intermediate frequency electromagnetic waves is 600 kHz, and the frequency range of the high frequency electromagnetic waves is 300 MHz. .
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are both square waves, and the electromagnetic wave propagation directions of the two are the same.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • Embodiment 4 Method for preparing multi-pole micro-kinetic energy drinking water of the present invention
  • non-contact electromagnetic waves of low, medium and high frequencies are applied, wherein the frequency of the low frequency electromagnetic waves is 75 kHz, the frequency range of the intermediate frequency electromagnetic waves is 720 kHz, and the frequency range of the high frequency electromagnetic waves is 425 MHz. .
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are sharp waves, and the electromagnetic wave propagation directions of the two are the same.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • the multi-pole micro-kinetic energy drinking water prepared in Examples 1-4 was tested according to the Standard Test Method for Drinking Water (GB/T 5750-2006), and the test results were basically the same. The results are shown in Table 1.
  • the indicators of the micro-kinetic energy drinking water of the present invention all meet the requirements of the Sanitary Standard for Drinking Water (GB5749-2006).
  • test substance was set at a dose of 20000 mg/kg body weight, and 10 female and male animals. Grouped by sex. Drip by 0.1ml/10g body weight by oral gavage Toxic amount.
  • the oral LD 50 of the test article for both female and male mice is greater than 10000 mg/kg body weight, which is an actual non-toxic grade.
  • Plasmid DNA pET28a plasmid was amplified and extracted in DH5a E. coli (plasmid extraction kit was purchased from Promega);
  • EDTA is provided by Sinopharm Chemical Reagent Co., Ltd.;
  • Electrophoresis instrument Liuyi Instrument Factory
  • Test substance Micro-kinetic energy drinking water prepared in Examples 3 and 4
  • Group I untreated group, adding untreated sterile water to the reaction system;
  • Group II the kinetic energy drinking water prepared in Example 3;
  • Group III The kinetic energy drinking water prepared in Example 4.
  • reaction materials were added in the following order, and the final volume of the reaction system was 10 ⁇ L:
  • reaction was stopped by adding 1 ⁇ L of 5 mM EDTA and 2.2 ⁇ L of loading buffer at 0.5 hour and 1 hour, respectively.
  • the redox reaction between vitamin C and divalent copper ions is accompanied by the formation of peroxide, which causes damage to the plasmid DNA, which is reflected in the weakening of the electrophoresis band.
  • the results of this experiment are shown in Figure 1.
  • the experiments were divided into three groups. The grouping method is described in the scheme. The two time points of each group were: 0.5 hours and 1 hour, and the brightness of DNA represented the DNA content.
  • the rate of degradation of plasmid DNA by redox reaction was basically the same in group II and group III compared with untreated group I, and there was no effect of preventing or accelerating DNA damage and cleavage due to water treatment.
  • the redox reaction between vitamin C and divalent copper ions is accompanied by the formation of peroxide, which causes damage to the plasmid DNA, which is reflected in the weakening of the electrophoresis band.
  • the results of this experiment showed that the rate of degradation of plasmid DNA by redox reaction was basically the same in group II and group III compared with untreated group I, and there was no industrial effect of preventing or accelerating DNA damage and cleavage due to water treatment. It is indicated that the multipolar micro kinetic energy of the present invention has no effect on peroxide-induced DNA damage.
  • L.1 human liver cancer cells HepG2;
  • FAM-aptamer purchased from Shanghai Shenggong Bioengineering Co., Ltd.;
  • the cell culture medium adherently cultured in a 96-well plate was changed to medium A and medium B containing FAM-C6-8 aptamer, cultured at 37 ° C for 30 min, washed once with PBS, and observed under a fluorescence microscope.
  • Fig. 2 There is no difference in cell morphology between the treated group and the untreated group.
  • the cells are white-fluorescent, the cell membrane is intact, and no obvious FAM-labeled C6-8aptamer green fluorescence is observed in the cells.
  • the FAM-labeled C6-8 aptamer can enter the cell and bind to the protein in the cell, and emit green fluorescence after excitation at 530 wavelength.
  • the results in the figure show that there is no difference between the cells in the treated group and the untreated group, all of which are autofluorescence, indicating that the cell membrane is intact, and the multipolar microkinetic energy technology of the present invention has no effect on cell morphology and cell membrane integrity.
  • Glacier Mineral Water Purchased at Carrefour Supermarket
  • Ultrapure water made by the German Sartorius ultrapure water preparation system
  • Example 3 The ordinary drinking water raw water used in Example 3 and the micro-kinetic energy drinking water prepared in Example 3 were placed in an experimental vessel, and the absorption of the spectral light spectrum of the standard light source of the sample of 200 nm to 800 nm was scanned, and the experimental results were recorded.
  • Example 3 The micro-kinetic energy prepared in Example 3 is placed in an ultraviolet intensity detecting system, the ultraviolet light source illuminates the water to be tested, and the photodetector Thorlab DET10A/M is read by an oscilloscope MS04104 to read ultraviolet rays that have not passed the water to be tested.
  • the intensity value is read by the value of the ultraviolet intensity of the water to be tested, continuously tested continuously for 72 hours, and the amount of change in ultraviolet intensity during the recording.
  • Example 3 The drinking water made from the Vietnamese glacial mineral water in the same manner as in Example 3 is placed in the ultraviolet intensity detecting system, and the step (2) is repeated to record the amount of change in the ultraviolet intensity during the recording.
  • Multi-pole micro-kinetic energy drinking water made from ordinary water
  • Multi-pole micro-kinetic energy drinking water made from Evian natural mineral water
  • Multi-pole micro-kinetic energy drinking water made from Vietnamese glacial mineral water
  • the absorption spectrum absorption peak of the multi-pole micro-kinetic energy drinking water of the present invention is shifted to a short wave by about 30 nm;
  • Glacier Mineral Water Purchased at Carrefour Supermarket
  • Ultrapure water made by the German Sartorius ultrapure water preparation system
  • Test environment temperature 22 ° C, humidity 37%.
  • Example 3 The micro-kinetic energy prepared in Example 3 is placed in a sample cell of a fiber optic spectrometer, and an excitation pulse is given to the sample by a 266 nm pulsed ultraviolet laser, and the spectral spectrum of the sample is used to measure the emission spectrum of the sample. Fluorescence emission spectrum of the sample.
  • Example 3 The ordinary drinking water raw water used in Example 3 was placed in the fiber optic spectrometer sample cell, and step 2 was repeated to record the experimental data.
  • Example 10 The same method as in Example 3 will be used to treat the Vietnamese glacial mineral water. Three samples of drinking water were placed in the fiber optic spectrometer sample cell, and the experimental data was recorded in steps 2 and 3.
  • the fluorescence steady-state transient test results of ordinary drinking water and multi-pole micro-kinetic drinking water made by it are shown in Fig. 6.
  • the fluorescence steady-state transient test results of Evian natural mineral water and its multi-pole micro-kinetic drinking water are shown in Figure 7.
  • the fluorescence steady-state transient test results of Vietnamese glacial mineral water and multi-pole micro-kinetic drinking water made by it are shown in Fig. 8.
  • the glacial mineral water in Moscow was tested for repeatability by taking three samples of multi-polar micro-kinetic energy drinking water made of raw water. The results are shown in Figure 9.
  • Example 9 Multi-polar kinetic energy drinking water of the present invention for preventing obesity model rat body weight, The effect of body fat content
  • the multi-pole micro-kinetic energy drinking water (kinetic energy water) prepared in Example 3 is a colorless and odorless transparent aqueous liquid, which is stored at room temperature and used within 72 hours after preparation;
  • Feeding conditions Experimental animal facilities continue to maintain barrier environmental standards.
  • the control range of the main environmental indicators room temperature 20 ⁇ 26 ° C, the daily temperature difference ⁇ 4 ° C.
  • the relative humidity is 40 to 70%.
  • Minimum air exchange times 15 times / hour, light illumination: dark 12h: 12h.
  • the animals are housed in a polypropylene rat group cage.
  • the cage specifications are: 545*395*200mm 3 , 3 to 5 per box, and the feeding space meets the minimum required for experimental animals in the national standard GB14925-2010 of the People's Republic of China. Space regulations. All animals are managed by trained personnel. Replace the litter and cage once a week. Rat-specific feed is added daily for animal consumption, and the animal's diet is kept free during the whole feeding process.
  • Baoding appliances should be of reasonable structure, appropriate specifications, sturdy and durable, environmentally friendly and easy to operate. The mandatory restrictions on the animal's body should be reduced to a minimum without affecting the experiment.
  • Sample collection The operation of collecting samples for experimental animals in a safe and humane manner (minimizing the tension and discomfort caused to animals).
  • the body weight was randomly divided into 2 groups, 10 rats were given normal animal feed as a blank control group, and 40 rats were given high-calorie feed as a model group. The amount of food and the amount of food left were recorded weekly, and the body weight was weighed once. After 2 weeks of feeding, rats given high-calorie diet were sorted by weight gain.
  • model control group received normal drinking water daily (normal daily drinking water 1.5mL/100g body weight); kinetic energy low dose group daily drinking kinetic energy water (drinking bottle) Filling kinetic energy, change once a day); kinetic energy high-dose group, in addition to daily basic kinetic energy, daily kinetic energy, the dose is 1.5mL/100g body weight; Bishengyuan slimming tea group dose is 1.0g /kg (Beishengyuan slimming tea clinical person daily dosage 10g, calculated according to 60kg, 0.167g / kg, calculated according to 10 times dose, equivalent to 1.67g / kg), the dosage volume is 1.5mL / 100g body weight, the preparation concentration is 0.111g /mL. There were no fasting and water prohibition requirements during the experiment. Each test sample was continuously administered for 30 days.
  • the body weight was weighed, 1% sodium pentobarbital (0.5 ml/100 g body weight) was anesthetized, the fat around the kidney, the fat around the testicles were dissected, and weighed to calculate the fat/body ratio.
  • the weight results were processed using SPSS statistical software and compared between the drug-administered group and the control group. According to the following method: the Kolmogorov-Smirnov method is used for the normal test, and the Levene median method is used to test the homogeneity of variance. If P>0.05, the one-way ANOVA method is used, if the normal and the variance are homogeneous. The sex test failed (P ⁇ 0.05) and a non-parametric Mann-Whitney test was performed.
  • the weight gain or weight gain of the experimental group was lower than that of the model control group.
  • the body fat weight or lipid/body ratio was lower than that of the model control group, and the difference was significant.
  • the food intake was not significantly lower than the model control group, and the test sample could be determined.
  • Animal weight loss function test results were positive. Or analyze the results according to the actual conditions of the test object.
  • the peripheral fat weight and liposome ratio of the model group at the end of the experiment (3.43 ⁇ 0.34**, vs 2.51 ⁇ 0.64, g, **p ⁇ 0.01; 0.780 ⁇ 0.065** , vs 0.567 ⁇ 0.154, %, ** p ⁇ 0.01), testicular fat weight and fat body ratio (8.49 ⁇ 1.21**, Vs 6.49 ⁇ 1.14, g, **p ⁇ 0.01; 1.929 ⁇ 0.220**, vs 1.457 ⁇ 0.216,%, **p ⁇ 0.01,) significantly increased with the blank control group, indicating that eating high-calorie feed resulted in rat kidney, The weight of the testicles and the ratio of fat to body increased.
  • the weight of the peri-renal fat and the ratio of fat to body in the low- and high-dose kinetic energy water (2.99 ⁇ 0.67, 2.77 ⁇ 0.45*, vs 3.43 ⁇ 0.34, g; 0.657 ⁇ 0.142*, 0.622 ⁇ 0.085**, vs 0.780 ⁇ 0.065,%;*p ⁇ 0.05, **p ⁇ 0.01) was significantly reduced, and had a dose-effect relationship; compared with the model group, the weight of the testicular fat and the ratio of fat to body in the high-dose group of kinetic energy (7.59 ⁇ 1.16, Vs 8.49 ⁇ 1.21, g; 1.707 ⁇ 0.222 *, vs 1.929 ⁇ 0.220, %; * p ⁇ 0.05) decreased, suggesting that kinetic energy has a role in reducing the fat weight and fat ratio of specific parts of the body (kidney and testis).
  • the periplasmic fat weight and liposome ratio of the Bishengyuan slimming tea group (2.37 ⁇ 0.58**, vs 3.43 ⁇ 0.34, g; 0.551 ⁇ 0.118**, vs 0.780 ⁇ 0.065,%; **p ⁇ 0.01)
  • the periplasmic fat weight and fat body ratio of Besunyen slimming tea group (7.21 ⁇ 1.61, vs 8.49 ⁇ 1.21, g; 1.675 ⁇ 0.296*, vs 1.929 ⁇ 0.220,%; *p ⁇ 0.05) decreased
  • Besunyen slimming tea has the effect of reducing the fat weight and fat ratio of specific parts (kidney and testis) in rats.
  • the multi-polar micro-kinetic energy drinking water of the present invention has the effect of lowering the fat weight and the fat ratio of specific parts (kidney and testis) in the body.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Hydrology & Water Resources (AREA)
  • Organic Chemistry (AREA)
  • Water Supply & Treatment (AREA)
  • Environmental & Geological Engineering (AREA)
  • Toxicology (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

多极微动能饮用水在制备用于减肥的饮料、保健品或药物中的用途，所述多极微动能饮用水是采用低、中、高三种不同频率的电磁波非接触式处理饮用水原水制备得到的。

Description

多极微动能饮用水在制备用于减肥的饮料、保健品或药物中的用途 技术领域
本发明属于水处理技术领域，具体涉及一种多极微动能饮用水在制备用于减肥的饮料、保健品或药物中的用途。
背景技术
水(H2O)是由氢、氧两种元素组成的无机物，在常温常压下为无色无味的透明液体。水是最常见的物质之一，是包括人类在内所有生命生存的重要资源，也是生物体最重要的组成部分。水在生命演化中起到了重要的作用。人类很早就开始对水产生了认识，东西方古代朴素的物质观中都把水视为一种基本的组成元素。
现代人以科学的角度对水有如下认识：
水分子具有记忆力：当科学家将个别具有不同形状和重量的雪花晶体分别溶化而后再冻结起来时，发现重新冻结的雪花晶体的形状和重量与溶化前完全一样，得出水本身有“记忆力”的结论。
由于水分子本身有其特殊的结构形状，它是由两个氢和一个氧离子以104.5的角度结合而成。它是一种带有电子“磁极性”的离子化系统。水分子在零度及零度以下呈现固态的结晶体，我们称之为冰。然而，水在零至60℃的温度下并不是完全呈现液状，而是以一种液态晶体的形式存在。但如果温度高于60℃，这液态晶体分子便会遭受破坏。
由于水分子的特殊夹角方式，可以将稀释过的溶质分子的形体包含在它的液态晶体中，这就是一般所理解的溶解过程。现在的新发现是，当用某种办法使溶质分子与水分子分离时(如经由多次的稀释与震荡)，水分子仍旧能够保持对溶质分子的“形状”记忆。当机体细胞接触到这样的水分子时，会把这个溶质分子的“形状”记忆当作真的溶质分子一样对待，这是有200多年历史的顺势疗法的基石。
水由氢与氧两种元素结合而成，但不以单个的水分子存在于自然界，而是由许多个水分子集聚成水分子的集团(H2O)n存在于自然界(即上述的液态结晶)，水分子的集团可能呈链状、环状、团状或葡萄状，最少五个水分子，多则十几、数十至数百个。
目前已经有采用电磁波来处理水分子，以提高水分子的能量等级或者消毒或者预防和清除污垢的方法。但是这些处理方法均不能很好地控制处理的效果，且处理后的水的效果大多仅限于饮用，没有其他的附加的有利效果。
发明内容
为了克服现有技术的缺陷，本发明提供一种采用电磁波非接触式处理得到的多极微动能饮用水及其制备方法和用途。
本发明的一个目的是提供一种多极微动能饮用水。本发明的另一个目的是提供一种上述多极微动能饮用水的制备方法。本发明的又一目的是提供一种上述多极微动能饮用水的用途。
本发明的上述目的是采用如下技术方案来实现的。
一方面，本发明提供一种多极微动能饮用水，该多极微动能饮用水由饮用水原水通过电磁波非接触式处理制备而成，与饮用水原水相比，紫外吸收峰向短波方向偏移25nm-40nm，优选向短波方向偏移28nm-30nm，最优选向短波方向偏移30nm或28nm。
与饮用水原水相比，本发明的多极微动能饮用水的荧光辐射光谱强度在300nm-400nm谱段均有大幅度提升，对同种样品重复实验表明，该实验有很好的重复性。
实验表明，本发明的多极微动能饮用水具有更多的核外电子处于高能级非辐射能级态。
优选地，所述饮用水原水为普通自来水或者各种矿泉水或纯净水，例如依云天然矿泉水、西藏冰川矿泉水、农夫山泉、娃哈哈等等。
本发明的多极微动能饮用水在制备好后72小时内饮用或者使用效果最佳。
另一方面，本发明提供一种上述多极微动能饮用水的制备方法，该制备方法包括采用低、中、高三种不同频率的电磁波非接触式处理饮用水原水，其中，低频电磁波的频率范围为30-100kHz，中频电磁波的频率范围为550-720kHz，高频电磁波的频率范围为300-725MHz。
优选地，所述饮用水原水为普通自来水或者各种矿泉水或纯净水，例如依云天然矿泉水、西藏冰川矿泉水、农夫山泉、娃哈哈等等。
优选地，所述低频电磁波的频率范围为45-75kHz。
优选地，所述中频电磁波的频率范围为600-720kHz。
优选地，所述高频电磁波的频率范围为300-425MHz。
优选地，所述低频电磁波和中频电磁波为正弦波、方波、尖波、锯齿波或梯形波。
优选地，所述低频电磁波和中频电磁波波形可以相同或者不同，优选相同。
优选地，所述低频电磁波和中频电磁波传播方向一致。
优选地，所述高频电磁波为三角波，且与所述低频电磁波和中频电磁波的传播方向垂直。
优选地，采用低、中、高三种不同频率的电磁波非接触式处理饮用水原水处理时，先使用低频电磁波和高频电磁波同时处理，然后再使用中频电磁波和高频电磁波同时处理。
优选地，使用低频电磁波和高频电磁波同时处理的时间为10-30分钟，优选为10-20分钟；使用中频电磁波和高频电磁波同时处理的时间为10-30分钟，优选为10-20分钟。
优选地，与饮用水原水相比，制备得到的多极微动能饮用水的紫外吸收峰向短波方向偏移25nm-40nm，优选向短波方向偏移28nm-30nm，最优选向短波方向偏移30nm或28nm。
与饮用水原水相比，本发明的多极微动能饮用水的荧光辐射光谱强度在300nm-400nm谱段均有大幅度提升，对同种样品重复实验表明，该实验有很好的重复性。
实验表明，本发明的多极微动能饮用水具有更多的核外电子处于高能级非辐射能级态。
在本发明中，产生电磁波的方法及装置是本领域的常规技术手段。
又一方面，本发明提供上述多极微动能饮用水在制备各种矿泉水、纯净水、饮料、保健品或药物中的用途。
优选地，所述饮料为功能饮料，可以用于抗疲劳、减肥、通便、降血压、降血糖、降血尿酸和血尿素。
优选地，所述保健品或药物可以用于抗疲劳、减肥、通便、降血压、降血糖、降血尿酸和降血尿素。
与现有技术相比，本发明采用电磁波非接触式处理水，没有添加任何添加剂，制作简单，生产效率高，成本低。
与已有的报道相比较，本发明的多极微动能饮用水较处理前的饮用水 原水在紫外吸收峰向短波方向产生了明显的位移，荧光辐射光谱强度在300nm-400nm谱段均有大幅度提升，更多的核外电子处于高能级非辐射能级态。动物实验已经证明具有抗疲劳、减肥、通便、降血压、降血糖、降血尿酸和血尿素的作用。
附图说明
以下，结合附图来详细说明本发明的实施方案，其中：
图1为不同处理组的水对过氧化物致DNA损伤的影响；
图2为不同处理组的培养基对细胞的影响；
图3为普通饮用水原水和使用该普通饮用水原水得到的本发明的多极微动能水的吸收光谱分布；
图4为依云天然矿泉水(原水)和使用该依云天然矿泉水得到的本发明的多极微动能水的吸收光谱分布；
图5为西藏冰川矿泉水(原水)和使用该西藏冰川矿泉水得到的本发明的多极微动能水的吸收光谱分布；
图6为普通饮用水(原水)与其制成的多极微动能饮用水的荧光稳态瞬态测试结果；
图7为依云天然矿泉水(原水)和与其制成的多极微动能饮用水的荧光稳态瞬态测试结果；
图8为西藏冰川矿泉水(原水)和与其制成的多极微动能饮用水的荧光稳态瞬态测试结果；
图9为西藏冰川矿泉水为原水制成的多极微动能饮用水分别取三个样品进行重复性测试结果；
图10为本发明的多极微动能饮用水对预防肥胖模型大鼠进食量的影响；
图11为本发明的多极微动能饮用水对预防肥胖模型大鼠体重的影响；
图12为本发明的多极微动能饮用水对预防肥胖模型大鼠肾脏、睾丸周围脂肪重量的影响；
图13为本发明的多极微动能饮用水对预防肥胖模型大鼠肾脏、睾丸周围脂肪脂体比的影响。
具体实施方式
下面结合具体实施方式对本发明进行进一步的详细描述，给出的实施例仅为了阐明本发明，而不是为了限制本发明的范围。
下述实施例中的实验方法，如无特殊说明，均为常规方法。下述实施例中所用的药材原料、试剂材料等，如无特殊说明，均为市售购买产品。
实施例1 本发明的多极微动能饮用水的制备方法
在普通饮用水原水管外，非接触式施加低、中、高三种不同频率的电磁波进行处理，其中，低频电磁波的频率为30kHz，中频电磁波的频率范围为550kHz，高频电磁波的频率范围为300MHz。
其中，所述低频电磁波和中频电磁波均为正弦波，且二者电磁波传播方向一致。所述高频电磁波为三角波，且与所述低频电磁波和中频电磁波的传播方向垂直。处理时，先使用低频电磁波和高频电磁波同时处理20分钟，然后再使用中频电磁波和高频电磁波同时处理20分钟。处理完成后，得到本发明的多极微动能饮用水。
实施例2 本发明的多极微动能饮用水的制备方法
在普通饮用水原水管外，非接触式施加低、中、高三种不同频率的电磁波进行处理，其中，低频电磁波的频率为100kHz，中频电磁波的频率范围为720kHz，高频电磁波的频率范围为725MHz。
其中，所述低频电磁波和中频电磁波均为方波，且二者电磁波传播方向一致。所述高频电磁波为三角波，且与所述低频电磁波和中频电磁波的传播方向垂直。处理时，先使用低频电磁波和高频电磁波同时处理10分钟，然后再使用中频电磁波和高频电磁波同时处理10分钟。处理完成后，得到本发明的多极微动能饮用水。
实施例3 本发明的多极微动能饮用水的制备方法
在普通饮用水原水管外，非接触式施加低、中、高三种不同频率的电磁波进行处理，其中，低频电磁波的频率为45kHz，中频电磁波的频率范围为600kHz，高频电磁波的频率范围为300MHz。
其中，所述低频电磁波和中频电磁波均为方波，且二者电磁波传播方向一致。所述高频电磁波为三角波，且与所述低频电磁波和中频电磁波的传播方向垂直。处理时，先使用低频电磁波和高频电磁波同时处理15分 钟，然后再使用中频电磁波和高频电磁波同时处理15分钟。处理完成后，得到本发明的多极微动能饮用水。
实施例4 本发明的多极微动能饮用水的制备方法
在普通饮用水原水管外，非接触式施加低、中、高三种不同频率的电磁波进行处理，其中，低频电磁波的频率为75kHz，中频电磁波的频率范围为720kHz，高频电磁波的频率范围为425MHz。
其中，所述低频电磁波和中频电磁波均为尖波，且二者电磁波传播方向一致。所述高频电磁波为三角波，且与所述低频电磁波和中频电磁波的传播方向垂直。处理时，先使用低频电磁波和高频电磁波同时处理20分钟，然后再使用中频电磁波和高频电磁波同时处理20分钟。处理完成后，得到本发明的多极微动能饮用水。
实施例5 本发明的多极微动能饮用水的理化指标检测
按照《生活饮用水标准检验方法》(GB/T 5750-2006)对实施例1-4制备的多极微动能饮用水进行检测，检测结果基本一致，结果见表1所示。
表1 本发明的多极微动能饮用水的理化指标检测结果
Figure PCTCN2015074748-appb-000001
Figure PCTCN2015074748-appb-000002
根据上表可知，本发明的微动能饮用水的指标均符合《生活饮用水卫生标准》(GB5749-2006)的要求。
实施例6 本发明的多极微动能饮用水的毒理实验
一、急性经口毒性试验
1、材料和方法
1.1 受试物：实施例3制备的微动能饮用水
1.2 动物：由中国食品药品检定研究院实验动物资源研究所提供昆明种小白鼠(合格证号：SCXK(京)2009-0017)，SPF级，体重18.0～22.0g，试验前，动物禁食过夜，自由饮水。
1.3 剂量分组：受试物设剂量为20000mg/kg体重，雌、雄性动物各10只。按性别分笼群饲。采用一次经口灌胃方式，按0.1ml/10g体重计算染 毒量。
1.4 观察指标：染毒后，观察动物的一般状况、中毒体征和死亡情况，观察期限两周。试验结束时所有动物进行解剖，记录动物的大体病理改变。根据急性毒性分级标准进行急性毒性分级。
2、试验结果
受试物对小鼠急性经口毒性试验结果
Figure PCTCN2015074748-appb-000003
3、结论
染毒后，动物无任何中毒症状及死亡情况出现，未见任何大体病理改变，动物体重呈增加趋势。因此，该受试物对雌、雄小白鼠经口LD50均大于10000mg/kg体重，属于实际无毒级。
二、对过氧化物致DNA损伤影响
1、实验材料与设备
1.1 5mM维生素C溶液：溶于三蒸水，现用现配，维生素C粉末由Sigma-Aldrich公司提供；
1.2 5mM CuCl2溶液：溶于三蒸水，现用现配，CuCl2粉末由国药集团化学试剂有限公司提供；
1.3 质粒DNA：pET28a质粒在DH5a大肠杆菌中扩增提取(质粒提取试剂盒购自Promega公司)；
1.4 5mMEDTA溶液pH8.0：EDTA由国药集团化学试剂有限公司提供；
1.5 10×PBS缓冲液：80gNaCl、2gKCl、14.4gNa2HPO4、2.4gKH2PO4溶解于蒸馏水，调pH值至7.4并定容至1L高压灭菌，所需试剂均购自国药集团化学试剂有限公司；
1.6 琼脂糖：由Biowest公司提供；
1.7 Goldview核酸染料由Biotium公司提供；
1.8 凝聚成像仪：由赛智创业科技有限公司提供；
1.9 电泳仪：六一仪器厂；
1.10 受试物：实施例3和4制备的微动能饮用水
2、实验方法
2.1 实验分组
I组：未处理组，采用未处理无菌水加入反应体系；
II组：实施例3制备的微动能饮用水；
III组：实施例4制备的微动能饮用水。
按照下列顺序加入各反应物质，反应体系终体积为10μL：
10×PBS缓冲液1μL；
质粒DNA(2μg)2μL；
5mM维生素C 1μL；
5mM CuC12 1μL；
用未处理水或处理水补齐至10μL。
2.2 分别于0.5小时和l小时，加入lμL 5mM EDTA和2.2μL上样缓冲液终止反应；
2.3 0.8％琼脂糠凝胶(含有Goldview染料)电泳l h；
2.4 凝胶成像。
3、实验结果
维生素C与二价铜离子发生氧化还原反应，伴随着过氧化物的生成，过氧化物会对质粒DNA造成损伤断裂，体现在电泳条带的减弱。本实验结果如图1所示，实验分为3组，分组方法见方案中描述，每组反应两个时间点:0.5小时和1小时，DNA的亮度代表DNA含量。根据实验结果显示，II组、III组与未处理I组相比较，质粒DNA受氧化还原反应降解的速率基本一致，并没有因为水的处理而产生阻止或者加速DNA损伤断裂的效应。
4、结果分析
维生素C与二价铜离子发生氧化还原反应，伴随着过氧化物的生成，过氧化物会对质粒DNA造成损伤断裂，体现在电泳条带的减弱。本实验结果显示，II组、III组与未处理I组相比较，质粒DNA受氧化还原反应降解的速率基本一致，并没有因为水的处理而产业阻止或者加速DNA损伤断裂的效应。说明本发明的多极微动能水对过氧化物致DNA损伤无影响。
三、对体外培养HepG2细胞膜完整性影响
1、实验材料与设备
l.1 人肝癌细胞：HepG2；
1.2 细胞培养基：DMEM培养液+10％FBS；
1.3 FAM-aptamer：购自上海生工生物工程有限公司；
1.4 荧光EZ微镜：OLYMPUS公司；
1.5 96孔细胞培养板；
2、实验方法
2.1 将对数生长期的细胞，加入适量胰酶消化。调整细胞浓度，以4000个细胞/孔的量接种于96孔培养板，过夜培养；
2.2 将5mL含10％FBS的DMEM培养基(培养基A)采用实施例3的方法处理40分钟(处理组)；
2.3 将5mL含10％FBS的DMEM培养基(均养基B)远离实施例3的电磁波源2米以上处理40分钟(未处理组)；
2.4 分别将4μg FAM标记的C6-8aptamer加入到100μL培养基A和培养基B中混匀；
2.5 将96孔板中贴壁培养的细胞液更换为含有FAM-C6-8aptamer的培养基A和培养基B，37℃培养30min后，用PBS洗一次，荧光显微镜下观察。
3、实验结果
结果如图2，处理组和未处理组中的细胞形态没有什么区别，都是细胞白发荧光，细胞胞膜完整，细胞内未观察到明显的FAM标记的C6-8aptamer绿色荧光。
4、结果与分析
如果细胞存在打孔则FAM标记的C6-8 aptamer可以进入细胞并与细胞内的蛋白结合，经530波长激发后发绿色荧光。而图中结果看到处理组和未处理组中的细胞没有什么区别，都是细胞自发荧光，说明细胞胞膜完整，本发明的多极微动能技术对细胞形态及细胞膜完整性无影响。
实施例7 本发明的多极微动能饮用水的宽光谱吸收率与紫外吸收率 变化
1、实验材料与设备
1.1 实施例3制备的微动能饮用水；
1.2 实施例3中使用的普通饮用水原水；
1.3 采用实施例3的相同的方法处理依云天然矿泉水制成的饮用水；
1.4 依云天然矿泉水：在家乐福超市购买；
1.5 采用实施例3的相同的方法处理西藏冰川矿泉水制成的饮用水；
1.6 西藏冰川矿泉水：在家乐福超市购买；
1.7 超纯水：由德国Sartorius超纯水制备系统制得；
1.8 光纤光谱仪Ava-Spec3648，测量范围200-1100nm，不确定度/准确度0.5nm；
1.9 示波器MS04104，设备出厂编号：C001163，测量精度：1GHz；
1.10 光电探测器Thorlab DET10A/M，设备出厂编号：JGZX-ZXZC-012，测量精度：200nm-1100nm；
1.11 测试条件：温度21℃，湿度：38％。
2、实验方法
2.1 宽光谱的吸收率
(1)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标稳定。
(2)将实施例3中使用的普通饮用水原水和实施例3制备的微动能饮用水置于实验器皿中，扫描200nm～800nm样品标准光源光谱分光的吸收，记录实验结果。
(3)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。
(4)将依云天然矿泉水和采用实施例3的相同的方法处理依云天然矿泉水制成的饮用水置于实验器皿中，重复步骤(2)，记录期间紫外线强度变化量。
(5)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。
(6)将西藏冰川矿泉水和采用采用实施例3的相同的方法处理西藏冰川矿泉水制成的饮用水置于实验器皿中，重复步骤(2)，记录期间紫外线强度变化量。
(7)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。
2.2 紫外吸收率测定
(1)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标稳定。
(2)将实施例3制备的微动能饮用水置于紫外线强度探测系统中，紫外光源照射待测水，通过示波器MS04104读取光电探测器Thorlab DET10A/M读取未经过待测水的紫外线强度数值，读取经过待测水的紫外线强度数值，连续不间断测试72小时，记录期间紫外线强度变化量。
(3)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。
(4)将采用实施例3的相同的方法处理依云天然矿泉水制成的饮用水置于紫外线强度探测系统中，重复步骤(2)，记录期间紫外线强度变化量。
(5)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。
(6)将采用实施例3的相同的方法处理西藏冰川矿泉水制成的饮用水置于紫外线强度探测系统中，重复步骤(2)，记录期间紫外线强度变化量。
(7)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。
3、实验结果
3.1 宽光谱吸收率测试
结果见图3-5。
3.2 紫外吸收率测定
普通水制成的多极微动能饮用水：
紫外吸收率：0.9135->0.9037
依云天然矿泉水制成的多极微动能饮用水：
紫外吸收率：0.9244->0.9188
西藏冰川矿泉水制成的多极微动能饮用水：
紫外吸收率：0.9157->0.9079
4、实验结论与分析
通过上述实验，得到如下结论：
(1)与原水相比较，本发明的多极微动能饮用水的吸收光谱吸收峰向短波偏移30nm左右；
(2)在72小时的测试下，本发明的多极微动能饮用水的吸收率下降1％。
实施例8 本发明的多极微动能饮用水的受激荧光光谱变化
1、实验材料与设备
1.1 实施例3制备的微动能饮用水；
1.2 实施例3中使用的普通饮用水原水；
1.3 采用实施例3的相同的方法处理依云天然矿泉水制成的饮用水；
1.4 依云天然矿泉水：在家乐福超市购买；
1.5 采用实施例3的相同的方法处理西藏冰川矿泉水制成的饮用水；
1.6 西藏冰川矿泉水：在家乐福超市购买；
1.7 超纯水：由德国Sartorius超纯水制备系统制得；
1.8 光纤光谱仪Ava-Spec3648，测量范围200-1100nm，不确定度/准确度0.5nm；
1.9 测试环境：温度22℃，湿度37％。
2、实验方法
(1)使用约200mL超纯水冲洗实验用器皿至各项物理化学指标稳定。
(2)将实施例3制备的微动能饮用水置于光纤光谱仪样品池中，用266nm脉冲紫外激光器给样品一个激发脉冲，使用光纤光谱仪光谱扫描功能，以对样品的发射光谱进行测量，记录样品的荧光发射光谱。
(3)使用约200mL超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。
(4)将实施例3中使用的普通饮用水原水置于光纤光谱仪样品池中，重复步骤2，记录实验数据。
(5)使用约200mL超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。
(6)将采用采用实施例3的相同的方法处理依云天然矿泉水制成的饮用水置于光谱仪样品池中，重复步骤2，3，记录实验数据。
(7)在将依云天然矿泉水置于光纤光谱仪样品池中，重复步骤2，3记录实验数据。
(8)将采用采用实施例3的相同的方法处理西藏冰川矿泉水制成的饮用水置光纤光谱仪样品池中，重复步骤2，3记录实验数据。
(9)将西藏冰川矿泉水置于光纤光谱仪样品池中，重复步骤2，3记录实验数据。
(10)将采用采用实施例3的相同的方法处理西藏冰川矿泉水制成的 饮用水取三个样品分别置于光纤光谱仪样品池中，重复步骤2，3记录实验数据。
3、实验结果
3.1 荧光稳态瞬态测试
普通饮用水与其制成的多极微动能饮用水的荧光稳态瞬态测试结果见图6。依云天然矿泉水和与其制成的多极微动能饮用水的荧光稳态瞬态测试结果见图7。西藏冰川矿泉水和与其制成的多极微动能饮用水的荧光稳态瞬态测试结果见图8。西藏冰川矿泉水为原水制成的多极微动能饮用水分别取三个样品进行重复性测试，结果见图9。
4、实验结论与分析
由上述实验结果可以看出，与原水相比较，本发明的多极微动能饮用水的三种不同样品的荧光辐射光谱强度在300nm-400nm谱段均有大幅度提升，对同种样品重复实验表明，该实验有很好的重复性。
实验表明，本发明的多极微动能水饮用水具有更多的核外电子处于高能级非辐射能级态。
实施例9 本发明的多极微动能饮用水对预防肥胖模型法大鼠体重、 体内脂肪含量的影响
1.材料和方法
1.1 样品：
实施例3制备的多极微动能饮用水(动能水)，无色无味透明水状液体，常温保存，制备好后72h内使用；
碧生源减肥茶，由北京澳特舒尔保健品开发有限公司生产，批号04131204。
1.2 实验动物：SD大鼠，购自北京华阜康生物科技股份有限公司，生产许可证号：SCXK(京)2009-0004，实验动物质量合格证号：11401300009312。50只，雄性，每组10只共5组，订购体重160-180g。
饲养条件：实验动物设施持续保持屏障环境标准。主要环境指标的控制范围：室温20～26℃，日温差≤4℃。相对湿度40～70％。最小换气次数15次/小时，光照明：暗＝12h：12h。动物饲养于聚丙烯大鼠群养盒中，笼具规格为：545*395*200mm3，每盒3～5只，其饲养空间符合中华人民共和国国家标准GB14925-2010中关于实验动物所需最小空间的规定。所有 动物均由培训合格的人员进行饲养管理。每周更换1次垫料和笼具。每日添加鼠专用饲料供动物食用，整个饲养过程中保持动物饮食活动自由。
动物福利：本试验所用动物及相关处置应符合动物福利的要求，实验开展前经过中国医学科学院北京协和医学院新药安全评价研究中心动物管理和使用委员会(IACUC)的审查批准。具体注意事项如下：
(1)实验过程中，应将动物的惊恐和疼痛减少到最低程度。
(2)在对实验动物进行手术、解剖时，必须进行有效的麻醉。术后恢复期应根据实际情况，进行镇痛和有针对性的护理及饮食调理。
(3)保定实验动物时，应避免引起动物的不安、惊恐、疼痛和损伤。保定器具应结构合理、规格适宜、坚固耐用、环保卫生、便于操作。在不影响实验的前提下，对动物身体的强制性限制宜减少到最低程度。
(4)样本采集：以安全人道的方式(尽量减少对动物造成的紧张与不适)对实验动物进行采集样本的操作。
(5)对于濒死动物、患病动物以及毒性反应严重的动物，应及时报告给兽医及专题负责人，给予及时治疗或实施安乐死。在不影响实验结果判定的情况下，选择“仁慈终点”，避免延长动物承受痛苦的时间。处死实验动物时，按照人道主义原则实施安死术。处死现场，无其他动物在场。确认动物死亡后，方可妥善处置尸体。
(6)职业安全方面：实验过程中，注意采取个人防护措施，如手套、隔离衣、面罩、眼罩、耳塞等。
1.3 剂量选择与受试物给予方式：
适应期结束后按体重随机分成2组，10只大鼠给予正常动物饲料作为空白对照组，40只给予高热量饲料作为模型组。每周记录给食量、剩食量，称量体重1次。喂养2周后，给予高热量饲料的大鼠按体重增重排序分组。
将40只肥胖模型大鼠按体重随机分成4组：模型对照组每日饮用正常饮用水(每天灌胃正常饮用水1.5mL/100g体重)；动能水低剂量组每日饮用动能水(饮水瓶灌装动能水，每天更换1次)；动能水高剂量组除每日基础饮用动能水外，每天灌胃动能水一次，剂量为1.5mL/100g体重；碧生源减肥茶组给药剂量为1.0g/kg(碧生源减肥茶临床人每天用量10g，按照60kg计算，为0.167g/kg，按照10倍剂量计算，折合1.67g/kg)，给药体积为1.5mL/100g体重，配制浓度为0.111g/mL。实验期间无禁食禁水要求。各受试样品连续给药30天。
1.4 主要仪器与试剂：电子天平，赛多利斯产品。
1.5 试验方法：
每周记录给食量、撒食量、剩食量，计算摄食量，称量体重1次。
试验结束后，称体重，1％戊巴比妥钠(0.5ml/100g体重)麻醉，解剖取肾周围脂肪、睾丸周围脂肪，并称重，计算脂/体比。
1.6 试验数据统计：
运用SPSS统计软件处理体重结果并进行给药组与对照组的比较分析。按照以下方法统计：采用Kolmogorov-Smirnov法进行正态检验，Levene中位数法进行方差齐性检验，若P＞0.05，进行单因素方差分析(One-Way ANOVA)方法，如果正态和方差齐性检验失败(P≤0.05)，进行非参数Mann-Whitney检验。
1.7 结果判定：
实验组的体重或体重增重低于模型对照组，体内脂肪重量或脂/体比低于模型对照组，差异有显著性，摄食量不显著低于模型对照组，可判定该受试样品动物减肥功能实验结果阳性。或者根据受试物实际情况进行结果分析。
2.结果
2.1 动能水对预防肥胖模型大鼠进食量的影响
表2 动能水对预防肥胖模型大鼠摄食量的影响(g/只/天)
Figure PCTCN2015074748-appb-000004
由表2和图10可见，动能水和阳性药碧生源减肥茶对模型大鼠的摄食量未见明显影响。
2.2 动能水对预防肥胖模型大鼠体重的影响
表3 动能水对预防肥胖模型大鼠体重的影响(g，
Figure PCTCN2015074748-appb-000005
n＝10)
Figure PCTCN2015074748-appb-000006
Figure PCTCN2015074748-appb-000007
由表3和图11可见，动能水和阳性药碧生源减肥茶对模型大鼠的体重未见明显影响。
2.3 动能水对预防肥胖模型大鼠肾脏、睾丸周围脂肪及脂体比的影响
表4、动能水对预防肥胖模型大鼠肾脏、睾丸周围脂肪重量的影响(g，
Figure PCTCN2015074748-appb-000008
n＝10)
Figure PCTCN2015074748-appb-000009
注：##，与空白对照比较，p<0.01；**，与模型对照比较，p<0.01
表5 动能水对预防肥胖模型大鼠肾脏、睾丸周围脂肪脂体比的影响(g，
Figure PCTCN2015074748-appb-000010
n＝10)
Figure PCTCN2015074748-appb-000011
注：##，与空白对照比较，p<0.01；*、**，与模型对照比较，p<0.05、p<0.01
由表4、5和图12、13可见，实验结束时模型组肾周围脂肪重量及脂体比(3.43±0.34**，vs 2.51±0.64，g，**p<0.01；0.780±0.065**，vs0.567±0.154，％，**p<0.01)、睾丸周围脂肪重量及脂体比(8.49±1.21**， vs 6.49±1.14，g，**p<0.01；1.929±0.220**，vs 1.457±0.216，％，**p<0.01，)与空白对照组显著增加，表明进食高热量饲料造成大鼠肾、睾丸周围脂肪重量及脂体比增加。
与模型组比较，体重动能水低、高剂量组肾周围脂肪重量及脂体比(2.99±0.67、2.77±0.45*，vs 3.43±0.34，g；0.657±0.142*、0.622±0.085**，vs 0.780±0.065，％；*p<0.05、**p<0.01)明显降低，且具有一定量效关系；与模型组比较，动能水高剂量组睾丸周围脂肪重量及脂体比(7.59±1.16，vs 8.49±1.21，g；1.707±0.222*，vs 1.929±0.220，％；*p<0.05)降低，提示动能水有降低大鼠体内特定部位(肾脏及睾丸)脂肪重量和脂体比的作用。
与模型组比较，碧生源减肥茶组肾周围脂肪重量及脂体比(2.37±0.58**，vs 3.43±0.34，g；0.551±0.118**，vs 0.780±0.065，％；**p<0.01)显著降低；与模型组比较，碧生源减肥茶组睾丸周围脂肪重量及脂体比(7.21±1.61，vs 8.49±1.21，g；1.675±0.296*，vs 1.929±0.220，％；*p<0.05)降低，提示碧生源减肥茶有降低大鼠体内特定部位(肾脏及睾丸)脂肪重量和脂体比的作用。
3.小结
在本实验条件下，SD大鼠连续饮用动能水30天，结果表明本发明的多极微动能饮用水有降低体内特定部位(肾脏及睾丸)脂肪重量和脂体比的作用。

Claims (10)

  1. 多极微动能饮用水在制备用于减肥的饮料、保健品或药物中的用途。
  2. 根据权利要求1所述的用途，其特征在于，所述多极微动能饮用水采用低、中、高三种不同频率的电磁波非接触式处理饮用水原水制备而成，其中，低频电磁波的频率范围为30-100kHz，中频电磁波的频率范围为550-720kHz，高频电磁波的频率范围为300-725MHz。
  3. 根据权利要求1或2所述的用途，其特征在于，所述饮用水原水为普通自来水或者各种矿泉水或纯净水，例如依云天然矿泉水、西藏冰川矿泉水、农夫山泉、娃哈哈等等。
  4. 根据权利要求1至3中任一项所述的用途，其特征在于，所述低频电磁波的频率范围为45-75kHz；
    优选地，所述中频电磁波的频率范围为600-720kHz；
    优选地，所述高频电磁波的频率范围为300-425MHz。
  5. 根据权利要求1至4中任一项所述的用途，其特征在于，所述低频电磁波和中频电磁波为正弦波、方波、尖波、锯齿波或梯形波；
    优选地，所述低频电磁波和中频电磁波波形相同或者不同；
    更优选地，所述低频电磁波和中频电磁波波形优选相同。
  6. 根据权利要求1至5中任一项所述的用途，其特征在于，所述低频电磁波和中频电磁波传播方向一致。
  7. 根据权利要求1至6中任一项所述的用途，所述高频电磁波为三角波，且与所述低频电磁波和中频电磁波的传播方向垂直。
  8. 根据权利要求1至7中任一项所述的用途，其特征在于，采用低、中、高三种不同频率的电磁波非接触式处理饮用水原水处理时，先使用低频电磁波和高频电磁波同时处理，然后再使用中频电磁波和高频电磁波同时处理；
    优选地，使用低频电磁波和高频电磁波同时处理的时间为10-30分钟，优选为10-20分钟；
    更优选地，使用中频电磁波和高频电磁波同时处理的时间为10-30分钟，优选为10-20分钟。
  9. 根据权利要求1至8中任一项所述的用途，其特征在于，与饮用 水原水相比，所述多极微动能饮用水的紫外吸收峰向短波方向偏移25nm-40nm，优选向短波方向偏移28nm-30nm，最优选向短波方向偏移30nm或28nm。
  10. 根据权利要求1至9中任一项所述的用途，其特征在于，所述饮料为功能饮料，可以用于减肥；
    优选地，所述保健品或药物可以用于减肥。
PCT/CN2015/074748 2015-03-20 2015-03-20 多极微动能饮用水在制备用于减肥的饮料、保健品或药物中的用途 WO2016149876A1 (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PCT/CN2015/074748 WO2016149876A1 (zh) 2015-03-20 2015-03-20 多极微动能饮用水在制备用于减肥的饮料、保健品或药物中的用途
AU2015387916A AU2015387916A1 (en) 2015-03-20 2015-03-20 Uses of multipolar microkinetic drinking water in preparing drink, healthcare product or medicament used for weight reduction

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2015/074748 WO2016149876A1 (zh) 2015-03-20 2015-03-20 多极微动能饮用水在制备用于减肥的饮料、保健品或药物中的用途

Publications (1)

Publication Number Publication Date
WO2016149876A1 true WO2016149876A1 (zh) 2016-09-29

Family

ID=56977846

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2015/074748 WO2016149876A1 (zh) 2015-03-20 2015-03-20 多极微动能饮用水在制备用于减肥的饮料、保健品或药物中的用途

Country Status (2)

Country Link
AU (1) AU2015387916A1 (zh)
WO (1) WO2016149876A1 (zh)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004057964A (ja) * 2002-07-30 2004-02-26 Shunzo Sumii 油性高溶解度を有する健康飲料水の製造方法
CN101456642A (zh) * 2009-01-09 2009-06-17 廖怀德 一种高磁活化水的生产方法
CN101466643A (zh) * 2006-05-29 2009-06-24 株式会社志贺机能水研究所 水的电磁场处理方法及电磁场处理装置
CN103058430A (zh) * 2012-12-18 2013-04-24 华南理工大学 一种高能小分子水的制备方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004057964A (ja) * 2002-07-30 2004-02-26 Shunzo Sumii 油性高溶解度を有する健康飲料水の製造方法
CN101466643A (zh) * 2006-05-29 2009-06-24 株式会社志贺机能水研究所 水的电磁场处理方法及电磁场处理装置
CN101456642A (zh) * 2009-01-09 2009-06-17 廖怀德 一种高磁活化水的生产方法
CN103058430A (zh) * 2012-12-18 2013-04-24 华南理工大学 一种高能小分子水的制备方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ZHANG, JIANPING ET AL.: "Progress in changing the structure of water clusters and the corresponding biological effects''.", JOURNAL OF TIANJIN INSTITUTE OF TECHNOLOGY., vol. 19, no. 4, 31 December 2003 (2003-12-31), pages 8 - 12 *

Also Published As

Publication number Publication date
AU2015387916A1 (en) 2017-11-09

Similar Documents

Publication Publication Date Title
Li et al. Mitochondrial transfer from bone marrow mesenchymal stem cells to motor neurons in spinal cord injury rats via gap junction
Zhang et al. Dietary iron oxide nanoparticles delay aging and ameliorate neurodegeneration in drosophila
Jiang et al. Sodium hydrosulfide attenuates cerebral ischemia/reperfusion injury by suppressing overactivated autophagy in rats
Chen et al. Melatonin protects against MPTP/MPP+‐induced mitochondrial DNA oxidative damage in vivo and in vitro
Xiang et al. Effect of silver nanoparticles on gill membranes of common carp: Modification of fatty acid profile, lipid peroxidation and membrane fluidity
Siedlecka-Kroplewska et al. Pterostilbene induces accumulation of autophagic vacuoles followed by cell death in HL60 human leukemia cells
Zhang et al. Near‐Infrared‐Enpowered Nanomotor‐Mediated Targeted Chemotherapy and Mitochondrial Phototherapy to Boost Systematic Antitumor Immunity
Li et al. Maslinic acid attenuates ischemia/reperfusion injury-induced myocardial inflammation and apoptosis by regulating HMGB1-TLR4 axis
Sandes et al. Evidence for the involvement of TNF-α, IL-1β and IL-10 in the antinociceptive and anti-inflammatory effects of indole-3-guanylhydrazone hydrochloride, an aromatic aminoguanidine, in rodents
Chen et al. Hydrogenated germanene nanosheets as an antioxidative defense agent for acute kidney injury treatment
Pilankar et al. A pro-oxidant combination of resveratrol and copper down-regulates hallmarks of cancer and immune checkpoints in patients with advanced oral cancer: Results of an exploratory study (RESCU 004)
Lien et al. Therapeutic potential of nanoceria pretreatment in preventing the development of urological chronic pelvic pain syndrome: Immunomodulation via reactive oxygen species scavenging and SerpinB2 downregulation
Yu et al. Protective effects of cynaroside on oxidative stress in retinal pigment epithelial cells
WO2016149876A1 (zh) 多极微动能饮用水在制备用于减肥的饮料、保健品或药物中的用途
Cheng et al. Splenocyte apoptotic pathway in mice following oral exposure to cerium trichloride
Xu et al. Alpha-mangostin attenuates oxidative stress and inflammation in adjuvant-induced arthritic rats
Guimarães et al. Mitochondrial pyruvate carrier as a key regulator of fever and neuroinflammation
WO2016149885A1 (zh) 多极微动能饮用水在制备用于降血尿酸的饮料、保健品或药物中的用途
WO2016149886A1 (zh) 多极微动能饮用水在制备用于降血尿素的饮料、保健品或药物中的用途
WO2016149879A1 (zh) 多极微动能饮用水在制备用于抗疲劳的饮料、保健品或药物中的用途
WO2016149883A1 (zh) 多极微动能饮用水在制备用于降血压的饮料、保健品或药物中的用途
WO2016149872A1 (zh) 一种多极微动能饮用水及其制备方法和用途
WO2016149882A1 (zh) 一种电磁波处理的饮用水在制备用于通便的饮料、保健品或药品中的用途
AU2015101911A4 (en) Uses of multipolar microkinetic drinking water in preparing drink, healthcare product or medicament used for weight reduction
AU2015101916A4 (en) Uses of multipolar microkinetic drinking water in preparing drink, healthcare product or medicament for use in reducing blood urea

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 15885815

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2015387916

Country of ref document: AU

Date of ref document: 20150320

Kind code of ref document: A

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC (EPO FORM 1205A DATED 25.01.2018)

122 Ep: pct application non-entry in european phase

Ref document number: 15885815

Country of ref document: EP

Kind code of ref document: A1