WO2016149886A1 - 多极微动能饮用水在制备用于降血尿素的饮料、保健品或药物中的用途 - Google Patents

多极微动能饮用水在制备用于降血尿素的饮料、保健品或药物中的用途 Download PDF

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WO2016149886A1
WO2016149886A1 PCT/CN2015/074765 CN2015074765W WO2016149886A1 WO 2016149886 A1 WO2016149886 A1 WO 2016149886A1 CN 2015074765 W CN2015074765 W CN 2015074765W WO 2016149886 A1 WO2016149886 A1 WO 2016149886A1
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frequency electromagnetic
electromagnetic wave
water
drinking water
kinetic energy
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PCT/CN2015/074765
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English (en)
French (fr)
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王卫星
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欢乐海（北京）水业科技有限公司
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Priority to AU2015387926A priority Critical patent/AU2015387926A1/en
Priority to PCT/CN2015/074765 priority patent/WO2016149886A1/zh
Publication of WO2016149886A1 publication Critical patent/WO2016149886A1/zh

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38—Other non-alcoholic beverages
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/48—Treatment of water, waste water, or sewage with magnetic or electric fields

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  • the invention belongs to the technical field of water treatment, and particularly relates to the use of multi-pole micro-kinetic energy drinking water in preparing beverages, health care products or medicines for reducing blood urea.
  • Water is an inorganic substance composed of two elements of hydrogen and oxygen, and is a colorless and odorless transparent liquid under normal temperature and normal pressure. Water is one of the most common substances, an important resource for all life, including human beings, and the most important component of living things. Water plays an important role in the evolution of life. Humans have long begun to understand water, and water is regarded as a basic component in the ancient and simple material view of the East and West.
  • the water molecule itself has its special structural shape, it is composed of two hydrogen and one oxygen ion combined at an angle of 104.5. It is an ionization system with an electronic "magnetic polarity". Water molecules exhibit solid crystals below zero and below zero, which we call ice. However, water is not completely liquid at a temperature of from zero to 60 ° C, but exists in the form of a liquid crystal. However, if the temperature is higher than 60 ° C, the liquid crystal molecules will be destroyed.
  • the form of the diluted solute molecules can be contained in its liquid crystals, which is a generally understood dissolution process.
  • the new discovery is that when a solute molecule is separated from a water molecule by some means (eg, through multiple dilutions and oscillations), the water molecule can still maintain a "shape" memory of the solute molecule. When the body cells are exposed to such water molecules, the "shape" memory of the solute molecule is treated as a true solute molecule, which is the cornerstone of homeopathic therapy with more than 200 years of history.
  • Water is composed of two elements of hydrogen and oxygen, but does not exist in nature as a single water molecule. Instead, a group of water molecules (H 2 O) n is present in nature (ie, the above liquid state). Crystallization), the group of water molecules may be chain, ring, agglomerate or grape-like, with at least five water molecules, and more than a dozen, tens to hundreds.
  • Electromagnetic waves have been used to treat water molecules to increase the energy level of water molecules or to disinfect or prevent and remove dirt.
  • these treatment methods do not control the effect of the treatment well, and the effect of the treated water is mostly limited to drinking, and there is no other additional advantageous effect.
  • the present invention provides a multi-pole micro-kinetic energy drinking water obtained by electromagnetic wave non-contact treatment, a preparation method thereof and use thereof.
  • the present invention provides a multi-pole micro-kinetic energy drinking water prepared by non-contact treatment of drinking water raw water by electromagnetic waves, and the ultraviolet absorption peak is short-waved compared with the raw water of drinking water.
  • the direction shift is 25 nm to 40 nm, preferably 28 nm to 30 nm in the short-wave direction, and most preferably 30 nm or 28 nm in the short-wave direction.
  • the spectral intensity of the fluorescent radiation of the multi-pole micro-kinetic energy drinking water of the invention is greatly improved in the spectrum of 300 nm to 400 nm, and repeated experiments on the same sample show that the experiment has good repeatability. .
  • the drinking water raw water is ordinary tap water or various kinds of mineral water or pure water, such as Evian natural mineral water, Cambodia glacier mineral water, Nongfu Spring, Wahaha, and the like.
  • the multi-pole micro-kinetic energy drinking water of the present invention is best consumed or used within 72 hours after preparation.
  • the present invention provides a method for preparing the above-mentioned multi-pole micro-kinetic energy drinking water, which comprises non-contact treatment of drinking water raw water by using electromagnetic waves of low, medium and high frequencies, wherein the frequency of low-frequency electromagnetic waves
  • the range is 30-100 kHz
  • the frequency range of the intermediate frequency electromagnetic wave is 550-720 kHz
  • the frequency range of the high frequency electromagnetic wave is 300-725 MHz.
  • the drinking water raw water is ordinary tap water or various kinds of mineral water or pure water, such as Evian natural mineral water, Cambodia glacier mineral water, Nongfu Spring, Wahaha, and the like.
  • the low frequency electromagnetic wave has a frequency in the range of 45-75 kHz.
  • the intermediate frequency electromagnetic wave has a frequency range of 600-720 kHz.
  • the high frequency electromagnetic wave has a frequency in the range of 300-425 MHz.
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are a sine wave, a square wave, a sharp wave, a sawtooth wave or a trapezoidal wave.
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave waveform may be the same or different, preferably the same.
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave propagate in the same direction.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • the low-frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed by the electromagnetic wave non-contact treatment of the drinking water raw water treatment by using three different frequencies of low, medium and high frequencies, and then the intermediate frequency electromagnetic wave and the high frequency electromagnetic wave are simultaneously processed.
  • the time for simultaneous treatment using the low frequency electromagnetic wave and the high frequency electromagnetic wave is 10-30 minutes, preferably 10-20 minutes; the time for simultaneous treatment using the intermediate frequency electromagnetic wave and the high frequency electromagnetic wave is 10-30 minutes, preferably 10-20 minutes. .
  • the prepared ultraviolet light absorption peak of the multi-pole micro-kinetic energy drinking water is shifted to the short-wave direction by 25 nm to 40 nm, preferably to the short-wave direction by 28 nm to 30 nm, and most preferably to the short-wave direction. 30 nm or 28 nm.
  • the spectral intensity of the fluorescent radiation of the multi-pole micro-kinetic energy drinking water of the invention is greatly improved in the spectrum of 300 nm to 400 nm, and repeated experiments on the same sample show that the experiment has good repeatability. .
  • a method and apparatus for generating electromagnetic waves are conventional techniques in the art.
  • the present invention provides the use of the above-described multi-pole micro-kinetic energy drinking water in the preparation of various mineral water, purified water, beverages, health care products or medicines.
  • the beverage is a functional beverage and can be used for anti-fatigue, weight loss, laxative, blood pressure lowering, blood sugar lowering, blood uric acid and blood urea.
  • the health care product or drug can be used for anti-fatigue, weight loss, laxative, blood pressure lowering, blood sugar lowering, blood uric acid and blood urea.
  • the invention adopts electromagnetic wave non-contact type treatment water, without adding any additives, is simple to manufacture, has high production efficiency and low cost.
  • the multi-pole micro-kinetic energy drinking water of the present invention is more than the drinking water before treatment.
  • the original water produced a significant shift in the ultraviolet absorption peak to the short-wave direction.
  • the spectral intensity of the fluorescent radiation was greatly improved in the 300-400 nm spectrum, and more extranuclear electrons were in the high-energy non-radiative energy level.
  • Animal experiments have proven to have anti-fatigue, weight loss, laxative, blood pressure lowering, blood sugar lowering, blood uric acid and blood urea reduction.
  • FIG 1 shows the effect of water in different treatment groups on peroxide-induced DNA damage
  • FIG. 1 shows the effect of differently treated media on cells
  • 3 is an absorption spectrum distribution of the ordinary drinking water raw water and the multi-pole micro kinetic energy of the present invention obtained by using the ordinary drinking water raw water;
  • Figure 6 is a fluorescence steady state transient test result of ordinary drinking water (raw water) and its multi-pole micro-kinetic energy drinking water;
  • Figure 7 is a fluorescence steady state transient test result of Evian natural mineral water (raw water) and multi-pole micro-kinetic energy drinking water made therewith;
  • Figure 8 shows the fluorescence steady-state transient test results of Vietnamese glacial mineral water (raw water) and multi-pole micro-kinetic energy drinking water made therewith;
  • Figure 9 shows the results of repeatability test of three samples of multi-pole micro-kinetic energy drinking water made from raw water of Georgia glacial mineral water;
  • Figure 10 shows the effect of kinetic energy on uric acid (UA) in hyperuricemia rats
  • Figure 11 shows the effect of kinetic energy on urea (UREA) in hyperuricemia rats
  • Figure 12 shows the effect of kinetic energy on creatinine (CREA) in hyperuricemia rats.
  • Embodiment 1 Method for preparing multi-pole micro-kinetic energy drinking water of the present invention
  • electromagnetic waves of three different frequencies of low, medium and high are applied in a non-contact manner, wherein the frequency of the low frequency electromagnetic wave is 30 kHz, the frequency range of the intermediate frequency electromagnetic wave is 550 kHz, and the frequency range of the high frequency electromagnetic wave is 300 MHz. .
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are both sinusoidal waves, and the electromagnetic wave propagation directions of the two are the same.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • non-contact electromagnetic waves of low, medium and high frequencies are applied, wherein the frequency of the low frequency electromagnetic waves is 100 kHz, the frequency range of the intermediate frequency electromagnetic waves is 720 kHz, and the frequency range of the high frequency electromagnetic waves is 725 MHz. .
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are both square waves, and the electromagnetic wave propagation directions of the two are the same.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • electromagnetic waves of low, medium and high frequencies are applied non-contactly, wherein the frequency of the low frequency electromagnetic waves is 45 kHz, the frequency range of the intermediate frequency electromagnetic waves is 600 kHz, and the frequency range of the high frequency electromagnetic waves is 300 MHz. .
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are both square waves, and the electromagnetic wave propagation directions of the two are the same.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • Embodiment 4 Method for preparing multi-pole micro-kinetic energy drinking water of the present invention
  • non-contact electromagnetic waves of low, medium and high frequencies are applied, wherein the frequency of the low frequency electromagnetic waves is 75 kHz, the frequency range of the intermediate frequency electromagnetic waves is 720 kHz, and the frequency range of the high frequency electromagnetic waves is 425 MHz. .
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are sharp waves, and the electromagnetic wave propagation directions of the two are the same.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • the multi-pole micro-kinetic energy drinking water prepared in Examples 1-4 was tested according to the Standard Test Method for Drinking Water (GB/T 5750-2006), and the test results were basically the same. The results are shown in Table 1.
  • the indicators of the micro-kinetic energy drinking water of the present invention all meet the requirements of the Sanitary Standard for Drinking Water (GB5749-2006).
  • Test substance Micro-kinetic energy drinking water prepared in Example 3
  • Animals Kunming species of white mice were provided by the Laboratory Animal Resources Research Institute of China Food and Drug Control Research Institute (Qualification No.: SCXK (Beijing) 2009-0017), SPF Grade, body weight 18.0 ⁇ 22.0g, before the test, the animals fasted overnight, free to drink water.
  • Dosage grouping The test substance is set at a dose of 20,000 mg/kg body weight, and each of the female and male animals is 10. Grouped by sex. The dose was calculated by oral gavage at 0.1 ml/10 g body weight.
  • the oral LD 50 of the test article for both female and male mice is greater than 10000 mg/kg body weight, which is an actual non-toxic grade.
  • Plasmid DNA pET28a plasmid was amplified and extracted in DH5a E. coli (plasmid extraction kit was purchased from Promega);
  • EDTA is provided by Sinopharm Chemical Reagent Co., Ltd.;
  • Electrophoresis Instrument Liuyi Instrument Factory
  • Test substance Micro-kinetic energy drinking water prepared in Examples 3 and 4
  • Group I untreated group, adding untreated sterile water to the reaction system;
  • Group II the kinetic energy drinking water prepared in Example 3;
  • Group III The kinetic energy drinking water prepared in Example 4.
  • reaction materials were added in the following order, and the final volume of the reaction system was 10 ⁇ L:
  • reaction was terminated by adding 1 ⁇ L of 5 mM EDTA and 2.2 ⁇ L of loading buffer at 0.5 hour and 1 hour, respectively.
  • the redox reaction between vitamin C and divalent copper ions is accompanied by the formation of peroxide, which causes damage to the plasmid DNA, which is reflected in the weakening of the electrophoresis band.
  • the results of this experiment are shown in Figure 1.
  • the experiments were divided into three groups. The grouping method is described in the scheme. The two time points of each group were: 0.5 hours and 1 hour, and the brightness of DNA represented the DNA content.
  • the rate of degradation of plasmid DNA by redox reaction was basically the same in group II and group III compared with untreated group I, and there was no effect of preventing or accelerating DNA damage and cleavage due to water treatment.
  • the redox reaction between vitamin C and divalent copper ions is accompanied by the formation of peroxide, which causes damage to the plasmid DNA, which is reflected in the weakening of the electrophoresis band.
  • the results of this experiment showed that the rate of degradation of plasmid DNA by redox reaction was basically the same in group II and group III compared with untreated group I, and there was no industrial effect of preventing or accelerating DNA damage and cleavage due to water treatment. It is indicated that the multipolar micro kinetic energy of the present invention has no effect on peroxide-induced DNA damage.
  • L.1 human liver cancer cells HepG2;
  • FAM-aptamer purchased from Shanghai Shenggong Bioengineering Co., Ltd.;
  • the cell culture medium adherently cultured in a 96-well plate was changed to medium A and medium B containing FAM-C6-8 aptamer, cultured at 37 ° C for 30 min, washed once with PBS, and observed under a fluorescence microscope.
  • Fig. 2 There is no difference in cell morphology between the treated group and the untreated group.
  • the cells are white-fluorescent, the cell membrane is intact, and no obvious FAM-labeled C6-8 aptamer green fluorescence is observed in the cells.
  • the FAM-labeled C6-8 aptamer can enter the cell and bind to the protein in the cell, and emit green fluorescence after excitation at 530 wavelength.
  • the results in the figure show that there is no difference between the cells in the treated group and the untreated group, all of which are autofluorescence, indicating that the cell membrane is intact, and the multipolar microkinetic energy technology of the present invention has no effect on cell morphology and cell membrane integrity.
  • Glacier Mineral Water Purchase at Carrefour supermarket;
  • ultrapure water made by the German Sartorius ultrapure water preparation system
  • Example 3 The ordinary drinking water raw water used in Example 3 and the micro-kinetic energy drinking water prepared in Example 3 were placed in an experimental vessel, and the absorption of the spectral light spectrum of the standard light source of the sample of 200 nm to 800 nm was scanned, and the experimental results were recorded.
  • Example 3 The micro-kinetic energy prepared in Example 3 is placed in an ultraviolet intensity detecting system, the ultraviolet light source illuminates the water to be tested, and the photodetector Thorlab DET10A/M is read by an oscilloscope MS04104 to read ultraviolet rays that have not passed the water to be tested. Intensity value, read the UV passing through the water to be tested Line strength value, continuous uninterrupted test for 72 hours, the amount of change in UV intensity during recording.
  • Example 3 The drinking water made from the Vietnamese glacial mineral water in the same manner as in Example 3 is placed in the ultraviolet intensity detecting system, and the step (2) is repeated to record the amount of change in the ultraviolet intensity during the recording.
  • Multi-pole micro-kinetic energy drinking water made from ordinary water
  • Multi-pole micro-kinetic energy drinking water made from Evian natural mineral water
  • Multi-pole micro-kinetic energy drinking water made from Vietnamese glacial mineral water
  • the absorption spectrum absorption peak of the multi-pole micro-kinetic energy drinking water of the present invention is shifted to a short wave by about 30 nm;
  • Glacier Mineral Water Purchase at Carrefour supermarket;
  • ultrapure water made by the German Sartorius ultrapure water preparation system
  • Test environment temperature 22 ° C, humidity 37%.
  • Example 3 The micro-kinetic energy prepared in Example 3 is placed in a sample cell of a fiber optic spectrometer, and an excitation pulse is given to the sample by a 266 nm pulsed ultraviolet laser, and the spectral spectrum of the sample is used to measure the emission spectrum of the sample. Fluorescence emission spectrum of the sample.
  • Example 3 The ordinary drinking water raw water used in Example 3 was placed in the fiber optic spectrometer sample cell, and step 2 was repeated to record the experimental data.
  • the fluorescence steady-state transient test results of ordinary drinking water and multi-pole micro-kinetic drinking water made by it are shown in Fig. 6.
  • the fluorescence steady-state transient test results of Evian natural mineral water and its multi-pole micro-kinetic drinking water are shown in Figure 7.
  • the fluorescence steady-state transient test results of Vietnamese glacial mineral water and multi-pole micro-kinetic drinking water made by it are shown in Fig. 8.
  • the glacial mineral water in Moscow was tested for repeatability by taking three samples of multi-polar micro-kinetic energy drinking water made of raw water. The results are shown in Figure 9.
  • Example 9 Multi-polar micro-kinetic energy drinking water of the present invention is high in potassium oxonate hyperuricemia SD Mouse influence
  • the multi-pole micro-kinetic energy drinking water (kinetic energy water) prepared in Example 3 is a colorless and odorless transparent aqueous liquid, which is stored at room temperature and used within 72 hours after preparation;
  • Feeding conditions Experimental animal facilities continue to maintain barrier environmental standards.
  • the control range of the main environmental indicators room temperature 20 ⁇ 26 ° C, the daily temperature difference ⁇ 4 ° C.
  • the relative humidity is 40 to 70%.
  • Minimum air exchange times 15 times / hour, light illumination: dark 12h: 12h.
  • the animals are housed in a polypropylene rat group box.
  • the cage specifications are: 545*395*200mm 3 , 5 per box, and the feeding space meets the minimum space required for experimental animals in the national standard GB14925-2010 of the People's Republic of China. Provisions. All animals are managed by trained personnel. Replace the litter and cage once a week. Rat-specific feed is added daily for animal consumption, and the animal's diet is kept free during the whole feeding process.
  • Baoding appliances should be of reasonable structure, appropriate specifications, sturdy and durable, environmentally friendly and easy to operate. The mandatory restrictions on the animal's body should be reduced to a minimum without affecting the experiment.
  • Sample collection The operation of collecting samples for experimental animals in a safe and humane manner (minimizing the tension and discomfort caused to animals).
  • Test indicators animal general observation, weight, growth status, blood uric acid, urea, creatinine.
  • Animal grouping and dose design Animals were quarantined for 2 weeks after receiving. At the end of quarantine, 70 animals were randomly divided into groups according to the results of blood uric acid determination, which were divided into 6 groups, 10 in each group. Ten animals were selected as the normal control group, and 50 animals were orally administered with 0.70 g/kg potassium oxonate (0.10 g/ml) per day to prepare a hyperuricemia model.
  • the blank control group drank normal drinking water daily; the model control group drank normal drinking water daily; the kinetic energy low dose group drank kinetic energy daily (drinking bottle filling kinetic energy, supplemented once a day); kinetic energy high dose group
  • the kinetic energy was administered once a day at a dose of 1.5 mL/100 g.
  • the positive drug was administered once daily at a dose of 10 mg/kg and the dose was 1.5 mL. /100g (Benzbroma
  • the dosage of adult tablets is 50mg per day, and the body weight is 0.833mg/kg according to 60kg.
  • the first day of administration (D15), the 34th day of administration (D34), and the 43rd day of administration (D43), blood uric acid, serum creatinine, and urea were measured four times. The first 3 times were collected by eyelids, and the last time the blood was collected from the abdominal aorta.
  • the weight results were processed using SPSS statistical software and compared between the drug-administered group and the control group. According to the following method: Kolmogorov-Smirnov method should be used for normal test, Levene median method for homogeneity test of variance, if P>0.05, one-way ANOVA method, if normal and variance The homogeneity test failed (P ⁇ 0.05), then a non-parametric Mann-Whitney test was required.
  • the effect of the uric acid function of the benzbromarone tablets was measured simultaneously with the determination of urea and creatinine levels, and the efficacy criteria of the drug-administered group were the same as above.

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Abstract

一种多极微动能饮用水在制备用于降血尿素的饮料、保健品或药物中的用途，该多极微动能饮用水由饮用水原水通过电磁波非接触式制备而成，与饮用水原水相比，紫外吸收峰向短波方向偏移25nm-40nm，优选偏移28nm-30nm，最优选偏移30nm或28nm。

Description

多极微动能饮用水在制备用于降血尿素的饮料、保健品或药物中的用途 技术领域
本发明属于水处理技术领域，具体涉及多极微动能饮用水在制备用于降血尿素的饮料、保健品或药物中的用途。
背景技术
水(H2O)是由氢、氧两种元素组成的无机物，在常温常压下为无色无味的透明液体。水是最常见的物质之一，是包括人类在内所有生命生存的重要资源，也是生物体最重要的组成部分。水在生命演化中起到了重要的作用。人类很早就开始对水产生了认识，东西方古代朴素的物质观中都把水视为一种基本的组成元素。
现代人以科学的角度对水有如下认识：
水分子具有记忆力：当科学家将个别具有不同形状和重量的雪花晶体分别溶化而后再冻结起来时，发现重新冻结的雪花晶体的形状和重量与溶化前完全一样，得出水本身有“记忆力”的结论。
由于水分子本身有其特殊的结构形状，它是由两个氢和一个氧离子以104.5的角度结合而成。它是一种带有电子“磁极性”的离子化系统。水分子在零度及零度以下呈现固态的结晶体，我们称之为冰。然而，水在零至60℃的温度下并不是完全呈现液状，而是以一种液态晶体的形式存在。但如果温度高于60℃，这液态晶体分子便会遭受破坏。
由于水分子的特殊夹角方式，可以将稀释过的溶质分子的形体包含在它的液态晶体中，这就是一般所理解的溶解过程。现在的新发现是，当用某种办法使溶质分子与水分子分离时(如经由多次的稀释与震荡)，水分子仍旧能够保持对溶质分子的“形状”记忆。当机体细胞接触到这样的水分子时，会把这个溶质分子的“形状”记忆当作真的溶质分子一样对待，这是有200多年历史的顺势疗法的基石。
水由氢与氧两种元素结合而成，但不以单个的水分子存在于自然界，而是由许多个水分子集聚成水分子的集团(H2O)n存在于自然界(即上述的液态结晶)，水分子的集团可能呈链状、环状、团状或葡萄状，最少五个水分子，多则十几、数十至数百个。
目前已经有采用电磁波来处理水分子，以提高水分子的能量等级或者消毒或者预防和清除污垢的方法。但是这些处理方法均不能很好地控制处理的效果，且处理后的水的效果大多仅限于饮用，没有其他的附加的有利效果。
发明内容
为了克服现有技术的缺陷，本发明提供一种采用电磁波非接触式处理得到的多极微动能饮用水及其制备方法和用途。
本发明的一个目的是提供一种多极微动能饮用水。本发明的另一个目的是提供一种上述多极微动能饮用水的制备方法。本发明的又一目的是提供一种上述多极微动能饮用水的用途。
本发明的上述目的是采用如下技术方案来实现的。
一方面，本发明提供一种多极微动能饮用水，该多极微动能饮用水由饮用水原水通过电磁波非接触式处理制备而成，与饮用水原水相比，紫外吸收峰向短波方向偏移25nm-40nm，优选向短波方向偏移28nm-30nm，最优选向短波方向偏移30nm或28nm。
与饮用水原水相比，本发明的多极微动能饮用水的荧光辐射光谱强度在300nm-400nm谱段均有大幅度提升，对同种样品重复实验表明，该实验有很好的重复性。
实验表明，本发明的多极微动能饮用水具有更多的核外电子处于高能级非辐射能级态。
优选地，所述饮用水原水为普通自来水或者各种矿泉水或纯净水，例如依云天然矿泉水、西藏冰川矿泉水、农夫山泉、娃哈哈等等。
本发明的多极微动能饮用水在制备好后72小时内饮用或者使用效果最佳。
另一方面，本发明提供一种上述多极微动能饮用水的制备方法，该制备方法包括采用低、中、高三种不同频率的电磁波非接触式处理饮用水原水，其中，低频电磁波的频率范围为30-100kHz，中频电磁波的频率范围为550-720kHz，高频电磁波的频率范围为300-725MHz。
优选地，所述饮用水原水为普通自来水或者各种矿泉水或纯净水，例如依云天然矿泉水、西藏冰川矿泉水、农夫山泉、娃哈哈等等。
优选地，所述低频电磁波的频率范围为45-75kHz。
优选地，所述中频电磁波的频率范围为600-720kHz。
优选地，所述高频电磁波的频率范围为300-425MHz。
优选地，所述低频电磁波和中频电磁波为正弦波、方波、尖波、锯齿波或梯形波。
优选地，所述低频电磁波和中频电磁波波形可以相同或者不同，优选相同。
优选地，所述低频电磁波和中频电磁波传播方向一致。
优选地，所述高频电磁波为三角波，且与所述低频电磁波和中频电磁波的传播方向垂直。
优选地，采用低、中、高三种不同频率的电磁波非接触式处理饮用水原水处理时，先使用低频电磁波和高频电磁波同时处理，然后再使用中频电磁波和高频电磁波同时处理。
优选地，使用低频电磁波和高频电磁波同时处理的时间为10-30分钟，优选为10-20分钟；使用中频电磁波和高频电磁波同时处理的时间为10-30分钟，优选为10-20分钟。
优选地，与饮用水原水相比，制备得到的多极微动能饮用水的紫外吸收峰向短波方向偏移25nm-40nm，优选向短波方向偏移28nm-30nm，最优选向短波方向偏移30nm或28nm。
与饮用水原水相比，本发明的多极微动能饮用水的荧光辐射光谱强度在300nm-400nm谱段均有大幅度提升，对同种样品重复实验表明，该实验有很好的重复性。
实验表明，本发明的多极微动能饮用水具有更多的核外电子处于高能级非辐射能级态。
在本发明中，产生电磁波的方法及装置是本领域的常规技术手段。
又一方面，本发明提供上述多极微动能饮用水在制备各种矿泉水、纯净水、饮料、保健品或药物中的用途。
优选地，所述饮料为功能饮料，可以用于抗疲劳、减肥、通便、降血压、降血糖、降血尿酸和血尿素。
优选地，所述保健品或药物可以用于抗疲劳、减肥、通便、降血压、降血糖、降血尿酸和血尿素。
与现有技术相比，本发明采用电磁波非接触式处理水，没有添加任何添加剂，制作简单，生产效率高，成本低。
与已有的报道相比较，本发明的多极微动能饮用水较处理前的饮用水 原水在紫外吸收峰向短波方向产生了明显的位移，荧光辐射光谱强度在300nm-400nm谱段均有大幅度提升，更多的核外电子处于高能级非辐射能级态。动物实验已经证明具有抗疲劳、减肥、通便、降血压、降血糖、降血尿酸和降血尿素作用。
附图说明
以下，结合附图来详细说明本发明的实施方案，其中：
图1为不同处理组的水对过氧化物致DNA损伤的影响；
图2为不同处理的培养基对细胞的影响；
图3为普通饮用水原水和使用该普通饮用水原水得到的本发明的多极微动能水的吸收光谱分布；
图4为依云天然矿泉水(原水)和使用该依云天然矿泉水得到的本发明的多极微动能水的吸收光谱分布；
图5为西藏冰川矿泉水(原水)和使用该西藏冰川矿泉水得到的本发明的多极微动能水的吸收光谱分布；
图6为普通饮用水(原水)与其制成的多极微动能饮用水的荧光稳态瞬态测试结果；
图7为依云天然矿泉水(原水)和与其制成的多极微动能饮用水的荧光稳态瞬态测试结果；
图8为西藏冰川矿泉水(原水)和与其制成的多极微动能饮用水的荧光稳态瞬态测试结果；
图9为西藏冰川矿泉水为原水制成的多极微动能饮用水分别取三个样品进行重复性测试结果；
图10为动能水对高尿酸血症大鼠尿酸(UA)的影响；
图11为动能水对高尿酸血症大鼠尿素(UREA)的影响；
图12为动能水对高尿酸血症大鼠肌酐(CREA)的影响。
具体实施方式
下面结合具体实施方式对本发明进行进一步的详细描述，给出的实施例仅为了阐明本发明，而不是为了限制本发明的范围。
下述实施例中的实验方法，如无特殊说明，均为常规方法。下述实施例中所用的药材原料、试剂材料等，如无特殊说明，均为市售购买产品。
实施例1 本发明的多极微动能饮用水的制备方法
在普通饮用水原水管外，非接触式施加低、中、高三种不同频率的电磁波进行处理，其中，低频电磁波的频率为30kHz，中频电磁波的频率范围为550kHz，高频电磁波的频率范围为300MHz。
其中，所述低频电磁波和中频电磁波均为正弦波，且二者电磁波传播方向一致。所述高频电磁波为三角波，且与所述低频电磁波和中频电磁波的传播方向垂直。处理时，先使用低频电磁波和高频电磁波同时处理20分钟，然后再使用中频电磁波和高频电磁波同时处理20分钟。处理完成后，得到本发明的多极微动能饮用水。
实施例2 本发明的多极微动能饮用水的制备方法
在普通饮用水原水管外，非接触式施加低、中、高三种不同频率的电磁波进行处理，其中，低频电磁波的频率为100kHz，中频电磁波的频率范围为720kHz，高频电磁波的频率范围为725MHz。
其中，所述低频电磁波和中频电磁波均为方波，且二者电磁波传播方向一致。所述高频电磁波为三角波，且与所述低频电磁波和中频电磁波的传播方向垂直。处理时，先使用低频电磁波和高频电磁波同时处理10分钟，然后再使用中频电磁波和高频电磁波同时处理10分钟。处理完成后，得到本发明的多极微动能饮用水。
实施例3 本发明的多极微动能饮用水的制备方法
在普通饮用水原水管外，非接触式施加低、中、高三种不同频率的电磁波进行处理，其中，低频电磁波的频率为45kHz，中频电磁波的频率范围为600kHz，高频电磁波的频率范围为300MHz。
其中，所述低频电磁波和中频电磁波均为方波，且二者电磁波传播方向一致。所述高频电磁波为三角波，且与所述低频电磁波和中频电磁波的传播方向垂直。处理时，先使用低频电磁波和高频电磁波同时处理15分钟，然后再使用中频电磁波和高频电磁波同时处理15分钟。处理完成后，得到本发明的多极微动能饮用水。
实施例4 本发明的多极微动能饮用水的制备方法
在普通饮用水原水管外，非接触式施加低、中、高三种不同频率的电磁波进行处理，其中，低频电磁波的频率为75kHz，中频电磁波的频率范围为720kHz，高频电磁波的频率范围为425MHz。
其中，所述低频电磁波和中频电磁波均为尖波，且二者电磁波传播方向一致。所述高频电磁波为三角波，且与所述低频电磁波和中频电磁波的传播方向垂直。处理时，先使用低频电磁波和高频电磁波同时处理20分钟，然后再使用中频电磁波和高频电磁波同时处理20分钟。处理完成后，得到本发明的多极微动能饮用水。
实施例5 本发明的多极微动能饮用水的理化指标检测
按照《生活饮用水标准检验方法》(GB/T 5750-2006)对实施例1-4制备的多极微动能饮用水进行检测，检测结果基本一致，结果见表1所示。
表1本发明的多极微动能饮用水的理化指标检测结果
Figure PCTCN2015074765-appb-000001
Figure PCTCN2015074765-appb-000002
根据上表可知，本发明的微动能饮用水的指标均符合《生活饮用水卫生标准》(GB5749-2006)的要求。
实施例6 本发明的多极微动能饮用水的毒理实验
一、急性经口毒性试验
1、材料和方法
1.1受试物：实施例3制备的微动能饮用水1.2动物：由中国食品药品检定研究院实验动物资源研究所提供昆明种小白鼠(合格证号：SCXK(京)2009-0017)，SPF级，体重18.0～22.0g，试验前，动物禁食过夜，自由饮水。
1.3剂量分组：受试物设剂量为20000mg/kg体重，雌、雄性动物各10只。按性别分笼群饲。采用一次经口灌胃方式，按0.1ml/10g体重计算染毒量。
1.4观察指标：染毒后，观察动物的一般状况、中毒体征和死亡情况，观察期限两周。试验结束时所有动物进行解剖，记录动物的大体病理改变。根据急性毒性分级标准进行急性毒性分级。
2、试验结果
受试物对小鼠急性经口毒性试验结果
Figure PCTCN2015074765-appb-000003
3、结论
染毒后，动物无任何中毒症状及死亡情况出现，未见任何大体病理改变，动物体重呈增加趋势。因此，该受试物对雌、雄小白鼠经口LD50均大于10000mg/kg体重，属于实际无毒级。
二、对过氧化物致DNA损伤影响
1、实验材料与设备
1.1 5mM维生素C溶液：溶于三蒸水，现用现配，维生素C粉末由Sigma-Aldrich公司提供；
1.2 5mM CuCl2溶液：溶于三蒸水，现用现配，CuCl2粉末由国药集团化学试剂有限公司提供；
1.3质粒DNA：pET28a质粒在DH5a大肠杆菌中扩增提取(质粒提取试剂盒购自Promega公司)；
1.4 5mMEDTA溶液pH8.0：EDTA由国药集团化学试剂有限公司提供；
1.5 10×PBS缓冲液：80gNaCl、2gKCl、14.4gNa2HPO4、2.4gKH2PO4溶解于蒸馏水，调pH值至7.4并定容至1L高压灭菌，所需试剂均购自国药集团化学试剂有限公司；
1.6琼脂糖：由Biowest公司提供；
1.7 Goldview核酸染料由Biotium公司提供；
1.8凝聚成像仪：由赛智创业科技有限公司提供；
1.9电泳仪：六一仪器厂；
1.10受试物：实施例3和4制备的微动能饮用水
2、实验方法
2.1实验分组
I组：未处理组，采用未处理无菌水加入反应体系；
II组：实施例3制备的微动能饮用水；
III组：实施例4制备的微动能饮用水。
按照下列顺序加入各反应物质，反应体系终体积为10μL：
10×PBS缓冲液1μL；
质粒DNA(2μg)2μL；
5mM维生素C 1μL；
5mM CuC12 1μL；
用未处理水或处理水补齐至10μL。
2.2分别于0.5小时和l小时，加入lμL 5mM EDTA和2.2μL上样缓冲液终止反应；
2.3 0.8％琼脂糠凝胶(含有Goldview染料)电泳lh；
2.4凝胶成像。
3、实验结果
维生素C与二价铜离子发生氧化还原反应，伴随着过氧化物的生成，过氧化物会对质粒DNA造成损伤断裂，体现在电泳条带的减弱。本实验结果如图1所示，实验分为3组，分组方法见方案中描述，每组反应两个时间点:0.5小时和1小时，DNA的亮度代表DNA含量。根据实验结果显示，II组、III组与未处理I组相比较，质粒DNA受氧化还原反应降解的速率基本一致，并没有因为水的处理而产生阻止或者加速DNA损伤断裂的效应。
4、结果分析
维生素C与二价铜离子发生氧化还原反应，伴随着过氧化物的生成，过氧化物会对质粒DNA造成损伤断裂，体现在电泳条带的减弱。本实验结果显示，II组、III组与未处理I组相比较，质粒DNA受氧化还原反应降解的速率基本一致，并没有因为水的处理而产业阻止或者加速DNA损伤断裂的效应。说明本发明的多极微动能水对过氧化物致DNA损伤无影响。
三、对体外培养HepG2细胞膜完整性影响
1、实验材料与设备
l.1人肝癌细胞：HepG2；
1.2细胞培养基：DMEM培养液+10％FBS；
1.3 FAM-aptamer：购自上海生工生物工程有限公司；
1.4荧光EZ微镜：OLYMPUS公司；
1.5 96孔细胞培养板；
2、实验方法
2.1将对数生长期的细胞，加入适量胰酶消化。调整细胞浓度，以4000个细胞/孔的量接种于96孔培养板，过夜培养；
2.2将5mL含10％FBS的DMEM培养基(培养基A)采用实施例3的方法处理40分钟(处理组)；
2.3将5mL含10％FBS的DMEM培养基(均养基B)远离实施例3的电磁波源2米以上处理40分钟(未处理组)；
2.4分别将4μg FAM标记的C6-8 aptamer加入到100μL培养基A和培养基B中混匀；
2.5将96孔板中贴壁培养的细胞液更换为含有FAM-C6-8 aptamer的培养基A和培养基B，37℃培养30min后，用PBS洗一次，荧光显微镜下观察。
3、实验结果
结果如图2，处理组和未处理组中的细胞形态没有什么区别，都是细胞白发荧光，细胞胞膜完整，细胞内未观察到明显的FAM标记的C6-8 aptamer绿色荧光。
4、结果与分析
如果细胞存在打孔则FAM标记的C6-8 aptamer可以进入细胞并与细胞内的蛋白结合，经530波长激发后发绿色荧光。而图中结果看到处理组和未处理组中的细胞没有什么区别，都是细胞自发荧光，说明细胞胞膜完整，本发明的多极微动能技术对细胞形态及细胞膜完整性无影响。
实施例7 本发明的多极微动能饮用水的宽光谱吸收率与紫外吸收率 变化
1、实验材料与设备
1.1实施例3制备的微动能饮用水；
1.2实施例3中使用的普通饮用水原水；
1.3采用实施例3的相同的方法处理依云天然矿泉水制成的饮用水；
1.4依云天然矿泉水：在家乐福超市购买；
1.5采用实施例3的相同的方法处理西藏冰川矿泉水制成的饮用水；
1.6西藏冰川矿泉水：在家乐福超市购买；
1.7超纯水：由德国Sartorius超纯水制备系统制得；
1.8光纤光谱仪Ava-Spec3648，测量范围200-1100nm，不确定度/准确度0.5nm；
1.9示波器MS04104，设备出厂编号：C001163，测量精度：1GHz；
1.10光电探测器Thorlab DET10A/M，设备出厂编号：JGZX-ZXZC-012，测量精度：200nm-1100nm；
1.11测试条件：温度21℃，湿度：38％。
2、实验方法
2.1宽光谱的吸收率
(1)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标稳定。
(2)将实施例3中使用的普通饮用水原水和实施例3制备的微动能饮用水置于实验器皿中，扫描200nm～800nm样品标准光源光谱分光的吸收，记录实验结果。
(3)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。
(4)将依云天然矿泉水和采用实施例3的相同的方法处理依云天然矿泉水制成的饮用水置于实验器皿中，重复步骤(2)，记录期间紫外线强度变化量。
(5)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。
(6)将西藏冰川矿泉水和采用采用实施例3的相同的方法处理西藏冰川矿泉水制成的饮用水置于实验器皿中，重复步骤(2)，记录期间紫外线强度变化量。
(7)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。
2.2紫外吸收率测定
(1)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标稳定。
(2)将实施例3制备的微动能饮用水置于紫外线强度探测系统中，紫外光源照射待测水，通过示波器MS04104读取光电探测器Thorlab DET10A/M读取未经过待测水的紫外线强度数值，读取经过待测水的紫外 线强度数值，连续不间断测试72小时，记录期间紫外线强度变化量。
(3)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。
(4)将采用实施例3的相同的方法处理依云天然矿泉水制成的饮用水置于紫外线强度探测系统中，重复步骤(2)，记录期间紫外线强度变化量。
(5)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。
(6)将采用实施例3的相同的方法处理西藏冰川矿泉水制成的饮用水置于紫外线强度探测系统中，重复步骤(2)，记录期间紫外线强度变化量。
(7)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。
3、实验结果
3.1宽光谱吸收率测试
结果见图3-5。
3.2紫外吸收率测定
普通水制成的多极微动能饮用水：
紫外吸收率：0.9135->0.9037
依云天然矿泉水制成的多极微动能饮用水：
紫外吸收率：0.9244->0.9188
西藏冰川矿泉水制成的多极微动能饮用水：
紫外吸收率：0.9157->0.9079
4、实验结论与分析
通过上述实验，得到如下结论：
(1)与原水相比较，本发明的多极微动能饮用水的吸收光谱吸收峰向短波偏移30nm左右；
(2)在72小时的测试下，本发明的多极微动能饮用水的吸收率下降1％。
实施例8 本发明的多极微动能饮用水的受激荧光光谱变化
1、实验材料与设备
1.1实施例3制备的微动能饮用水；
1.2实施例3中使用的普通饮用水原水；
1.3采用实施例3的相同的方法处理依云天然矿泉水制成的饮用水；
1.4依云天然矿泉水：在家乐福超市购买；
1.5采用实施例3的相同的方法处理西藏冰川矿泉水制成的饮用水；
1.6西藏冰川矿泉水：在家乐福超市购买；
1.7超纯水：由德国Sartorius超纯水制备系统制得；
1.8光纤光谱仪Ava-Spec3648，测量范围200-1100nm，不确定度/准确度0.5nm；
1.9测试环境：温度22℃，湿度37％。
2、实验方法
(1)使用约200mL超纯水冲洗实验用器皿至各项物理化学指标稳定。
(2)将实施例3制备的微动能饮用水置于光纤光谱仪样品池中，用266nm脉冲紫外激光器给样品一个激发脉冲，使用光纤光谱仪光谱扫描功能，以对样品的发射光谱进行测量，记录样品的荧光发射光谱。
(3)使用约200mL超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。
(4)将实施例3中使用的普通饮用水原水置于光纤光谱仪样品池中，重复步骤2，记录实验数据。
(5)使用约200mL超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。
(6)将采用采用实施例3的相同的方法处理依云天然矿泉水制成的饮用水置于光谱仪样品池中，重复步骤2，3，记录实验数据。
(7)在将依云天然矿泉水置于光纤光谱仪样品池中，重复步骤2，3记录实验数据。
(8)将采用采用实施例3的相同的方法处理西藏冰川矿泉水制成的饮用水置光纤光谱仪样品池中，重复步骤2，3记录实验数据。
(9)将西藏冰川矿泉水置于光纤光谱仪样品池中，重复步骤2，3记录实验数据。
(10)将采用采用实施例3的相同的方法处理西藏冰川矿泉水制成的饮用水取三个样品分别置于光纤光谱仪样品池中，重复步骤2，3记录实验数据。
3、实验结果
3.1荧光稳态瞬态测试
普通饮用水与其制成的多极微动能饮用水的荧光稳态瞬态测试结果见图6。依云天然矿泉水和与其制成的多极微动能饮用水的荧光稳态瞬态测试结果见图7。西藏冰川矿泉水和与其制成的多极微动能饮用水的荧光稳态瞬态测试结果见图8。西藏冰川矿泉水为原水制成的多极微动能饮用水分别取三个样品进行重复性测试，结果见图9。
4、实验结论与分析
由上述实验结果可以看出，与原水相比较，本发明的多极微动能饮用水的三种不同样品的荧光辐射光谱强度在300nm-400nm谱段均有大幅度提升，对同种样品重复实验表明，该实验有很好的重复性。
实验表明，本发明的多极微动能水饮用水具有更多的核外电子处于高能级非辐射能级态。
实施例9 本发明的多极微动能饮用水对氧嗪酸钾高尿酸血症SD大 鼠的影响
1.材料和方法
1.1样品：
实施例3制备的多极微动能饮用水(动能水)，无色无味透明水状液体，常温保存，制备好后72h内使用；
阳性药苯溴马隆，宜昌长江药业有限公司，批号0061402004。
1.2实验动物：SD大鼠，购自北京华阜康生物技术有限公司，生产许可证号：SCXK(京)2007-0001。70只，雄性，订购体重160～180g。经筛选后选择60只大鼠用于正式试验。
饲养条件：实验动物设施持续保持屏障环境标准。主要环境指标的控制范围：室温20～26℃，日温差≤4℃。相对湿度40～70％。最小换气次数15次/小时，光照明：暗＝12h：12h。动物饲养于聚丙烯大鼠群养盒中，笼具规格为：545*395*200mm3，每盒5只，其饲养空间符合中华人民共和国国家标准GB14925-2010中关于实验动物所需最小空间的规定。所有动物均由培训合格的人员进行饲养管理。每周更换1次垫料和笼具。每日添加鼠专用饲料供动物食用，整个饲养过程中保持动物饮食活动自由。
动物福利：本试验所用动物及相关处置应符合动物福利的要求，实验开展要经过中国医学科学院北京协和医学院新药安全评价研究中心动物 管理和使用委员会(IACUC)的审查批准。具体注意事项如下：
(1)实验过程中，应将动物的惊恐和疼痛减少到最低程度。
(2)在对实验动物进行手术、解剖时，必须进行有效的麻醉。术后恢复期应根据实际情况，进行镇痛和有针对性的护理及饮食调理。
(3)保定实验动物时，应避免引起动物的不安、惊恐、疼痛和损伤。保定器具应结构合理、规格适宜、坚固耐用、环保卫生、便于操作。在不影响实验的前提下，对动物身体的强制性限制宜减少到最低程度。
(4)样本采集：以安全人道的方式(尽量减少对动物造成的紧张与不适)对实验动物进行采集样本的操作。
(5)对于濒死动物、患病动物以及毒性反应严重的动物，及时报告给兽医及专题负责人，给予及时治疗或实施安乐死。在不影响实验结果判定的情况下，选择“仁慈终点”，避免延长动物承受痛苦的时间。处死实验动物时，按照人道主义原则实施安死术。处死现场，无其他动物在场。确认动物死亡后，妥善处置尸体。
(6)职业安全方面：实验过程中，注意采取个人防护措施，如手套、隔离衣、面罩、眼罩、耳塞等。
1.4主要仪器与试剂：AU480全自动生化分析仪，血尿酸、尿素、肌酐测定试剂盒，中生试剂公司。
1.5试验方法：
1.5.1遵循的标准：杨桂梅等，大鼠高尿酸血症模型的建立，实验动物科学，2011年；刘淑芬等，高尿酸血症动物模型研究进展，基础医学与临床，2011年。
1.5.2检测指标：动物一般情况观察、体重、生长状况、血尿酸、尿素、肌酐。
1.5.3动物分组与剂量设计：动物接收后适应检疫2周，检疫结束时选取70只动物根据血尿酸测定结果进行随机分层分组，共分为6组，每组10只。选取10只动物作为正常对照组，50只动物每天灌胃给予0.70g/kg氧嗪酸钾(0.10g/ml)制备高尿酸血症模型。空白对照组每日饮用正常饮用水；模型对照组每日饮用正常饮用水；动能水低剂量组每日饮用动能水(饮水瓶灌装动能水，每日补充1次)；动能水高剂量组除每日基础饮用动能水外，每天灌胃动能水一次，剂量为1.5mL/100g；阳性药苯溴马隆组每日灌胃一次，给药剂量为10mg/kg，给药体积为1.5mL/100g(苯溴马 隆片成人每日用量50mg，人体重按照60kg计算，为0.833mg/kg，以人用量的10倍计算，折合10mg/kg,给药体积为1.5ml/kg，药物配制浓度为0.67mg/ml)；另设立动能水高剂量+苯溴马隆片组，用动能水配置苯溴马隆片，苯溴马隆片剂量也为10mg/kg，给药体积为1.5mL/100g。
表2动能水对氧嗪酸钾高尿酸血症SD大鼠尿酸、尿素、肌酐水平的影响的实验设计
Figure PCTCN2015074765-appb-000004
1.5.4给药时间：连续给药6周。
1.5.5测定时间：
给药前，给药第15天(D15)，给药第34天(D34)，给药第43天(D43)，共测定4次血尿酸、血肌酐、尿素。前3次采用眼龇采血，末次采用腹主动脉采血。
1.6试验数据统计：
运用SPSS统计软件处理体重结果并进行给药组与对照组的比较分析。按照以下方法统计：应采用Kolmogorov-Smirnov法进行正态检验，Levene中位数法进行方差齐性检验，若P＞0.05，进行单因素方差分析(One-Way ANOVA)方法，如果正态和方差齐性检验失败(P≤0.05)，那么需要进行非参数Mann-Whitney检验。
1.7结果判定：
结果判定：模型组动物尿酸值高于空白对照组动物或苯溴马隆组动物血尿酸明显低于模型对照组，差异具有显著性，表明高尿酸血症模型动物造模成功；动能水组实验动物血尿酸明显低于模型对照组，差异具有显著性，表明动能水具有降尿酸作用；若苯溴马隆加动能水组的尿酸值低于苯溴马隆组，则可判断动能水具有增强苯溴马隆片降尿酸功能的作用，同时进行尿素、肌酐水平的测定，给药组的效果判定标准同上。
2.结果：
表3动能水对氧嗪酸钾高尿酸血症SD大鼠血尿酸的影响(μmol/L，
Figure PCTCN2015074765-appb-000005
n＝10)
Figure PCTCN2015074765-appb-000006
注：**，与对照组比较，p<0.01；#、##与模型组比较，p<0.05、p<0.01
由表3、图10可见，药前各组别大鼠血尿酸(UA)结果较一致，均未见统计学差异。
连续给予氧嗪酸钾15天，与空白对照组比较，模型对照组动物的尿酸值有一定程度升高，有统计学差异(106.1±14.7**，vs 82.0±17.6，μmol/L，**p<0.01)；连续给予氧嗪酸钾34天，模型组氧嗪酸钾与空白对照组结果较一致，未见统计学差异(95.3±13.1，vs 98.4±19.5，μmol/L，p>0.05)，连续给药氧嗪酸钾43天，与空白对照组比较，模型组大鼠尿酸值明显升高，升高幅度为195.8％，统计学差异显著(216.2±20.1**，vs 73.1±11.0，μmol/L，**p<0.01)，说明氧嗪酸钾造模给药，造模过程中动物机体存在自我调节过程，连续给予氧嗪酸钾43天时，高尿酸血症模型造 模成功。
与模型对照组相比，大鼠连续饮用动能水43天，动能水低、高剂量组血尿酸值有一定程度的降低，降低幅度为8％，但差异虽未见显著性(198.6±19.0、198.7±17.6，vs 216.2±20.1，μmol/L，p>0.05)。苯溴马隆10mg/kg组、苯溴马隆片10mg/kg加动能水组血尿酸明显降低，差异具有显著性(139.9±30.5##、137.2±22.9##，vs 216.2±20.1，μmol/L，p>0.05)。
表4给药结束动能水对氧嗪酸钾高尿酸血症SD大鼠血尿素的影响(mmol/L，
Figure PCTCN2015074765-appb-000007
n＝10)
Figure PCTCN2015074765-appb-000008
注：**，与对照组比较，p<0.01；#、##与模型组比较，p<0.05、p<0.01
由表4、图11可见，连续给予氧嗪酸钾15天、34天，与空白对照组比较，模型对照组动物的尿素值与对照组绝对值较一致，未见统计学差异(D15：4.16±0.75，vs 4.49±0.62；D34：5.47±0.41，vs 5.89±0.78，mmol/L，p>0.05)，连续给药氧嗪酸钾43天，与空白对照组比较，模型组大鼠尿素值明显升高，升高幅度为67.9％，统计学差异显著(7.91±0.93**，vs 4.71±0.74，mmol/L，**p<0.01)，说明氧嗪酸钾造模给药，连续给予氧嗪酸钾43天时，高尿酸血症模型造模成功的同时，大鼠血尿素值也明显升高。
与模型对照组相比，大鼠连续饮用动能水43天，动能水低、高剂量组血尿素值明显降低，降低幅度分别为20.8％和35.7％，差异有显著性(6.26±1.33##、5.09±1.20##，vs 7.91±0.93，mmol/L，##p<0.01)。苯溴马隆 10mg/kg组、苯溴马隆片10mg/kg加动能水组血尿素值也明显降低，差异具有显著性(2.90±0.66##、2.53±0.29##，vs 7.91±0.93，mmol/L，##p<0.01)。
表5给药结束动能水对氧嗪酸钾高尿酸血症SD大鼠血肌酐的影响(μmol/L，
Figure PCTCN2015074765-appb-000009
n＝10)
Figure PCTCN2015074765-appb-000010
注：**，与对照组比较，*p<0.05；#、##与模型组比较，p<0.05、p<0.01
由表5、图12可见，连续给予氧嗪酸钾15天、34天，与空白对照组比较，模型对照组动物的血肌酐值与对照组绝对值较一致，未见统计学差异(D15：35.3±5.9，vs 35.4±5.3；D34：38.1±5.8，vs 40.1±5.7，μmol/L，p>0.05)，连续给药氧嗪酸钾43天，与空白对照组比较，模型组大鼠血肌酐值略有增加，增加幅度为10.2％，有统计学差异(38.4±4.3*，vs 34.9±1.5，mmol/L，*p<0.05)，说明氧嗪酸钾造模给药，连续给予氧嗪酸钾43天时，高尿酸血症模型造模成功的同时，大鼠血肌酐略有升高。
与模型对照组相比，大鼠连续饮用动能水43天，动能水低、高剂量组血肌酐值未见明显变化，差异未见显著性(41.0±1.9、40.1±2.1，vs 38.4±4.3，μmol/L，p>0.05)。苯溴马隆10mg/kg组、苯溴马隆片10mg/kg加动能水组血肌酐值未见明显变化，差异未见显著性(41.0±2.2、37.8±2.3，vs 38.4±4.3，μmol/L，p>0.05)。
3.小结
综合上述结果，在本实验条件下，连续饮用本发明的多极微动能饮用水43天，可轻一定程度降低氧嗪酸钾高尿酸SD大鼠的血尿酸值，可以明 显降低氧嗪酸钾高尿酸SD大鼠的血尿素值，对肌酐值没有影响。
说明动能水对氧嗪酸钾高尿酸SD大鼠升高的血尿酸具有一定程度的降低作用，同时可以明显降低氧嗪酸钾高尿酸SD大鼠升高的血尿素。

Claims (10)

  1. 多极微动能饮用水在制备用于降血尿素的饮料、保健品或药物中的用途。
  2. 根据权利要求1所述的用途，其特征在于，所述多极微动能饮用水采用低、中、高三种不同频率的电磁波非接触式处理饮用水原水制备而成，其中，低频电磁波的频率范围为30-100kHz，中频电磁波的频率范围为550-720kHz，高频电磁波的频率范围为300-725MHz。
  3. 根据权利要求1或2所述的用途，其特征在于，所述饮用水原水为普通自来水或者各种矿泉水或纯净水，例如依云天然矿泉水、西藏冰川矿泉水、农夫山泉、娃哈哈等等。
  4. 根据权利要求1至3中任一项所述的用途，其特征在于，所述低频电磁波的频率范围为45-75kHz；
    优选地，所述中频电磁波的频率范围为600-720kHz；
    优选地，所述高频电磁波的频率范围为300-425MHz。
  5. 根据权利要求1至4中任一项所述的用途，其特征在于，所述低频电磁波和中频电磁波为正弦波、方波、尖波、锯齿波或梯形波；
    优选地，所述低频电磁波和中频电磁波波形相同或者不同；
    更优选地，所述低频电磁波和中频电磁波波形优选相同。
  6. 根据权利要求1至5中任一项所述的用途，其特征在于，所述低频电磁波和中频电磁波传播方向一致。
  7. 根据权利要求1至6中任一项所述的用途，所述高频电磁波为三角波，且与所述低频电磁波和中频电磁波的传播方向垂直。
  8. 根据权利要求1至7中任一项所述的用途，其特征在于，采用低、中、高三种不同频率的电磁波非接触式处理饮用水原水处理时，先使用低频电磁波和高频电磁波同时处理，然后再使用中频电磁波和高频电磁波同时处理；
    优选地，使用低频电磁波和高频电磁波同时处理的时间为10-30分钟，优选为10-20分钟；
    更优选地，使用中频电磁波和高频电磁波同时处理的时间为10-30分钟，优选为10-20分钟。
  9. 根据权利要求1至8中任一项所述的用途，其特征在于，与饮用 水原水相比，所述多极微动能饮用水的紫外吸收峰向短波方向偏移25nm-40nm，优选向短波方向偏移28nm-30nm，最优选向短波方向偏移30nm或28nm。
  10. 根据权利要求1至9中任一项所述的用途，其特征在于，所述饮料为功能饮料，可以用于降血尿素；
    优选地，所述保健品或药物可以用于降血尿素。
PCT/CN2015/074765 2015-03-20 2015-03-20 多极微动能饮用水在制备用于降血尿素的饮料、保健品或药物中的用途 WO2016149886A1 (zh)

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Citations (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02131186A (ja) * 1988-11-10 1990-05-18 Fuji Keiki:Kk 簡易型水処理装置
CN1086497A (zh) * 1993-06-17 1994-05-11 高汝云 一种水的灭菌、提高品质及祛除水垢的方法
CN2758237Y (zh) * 2004-05-31 2006-02-15 周林 频谱水装置
CN1748856A (zh) * 2004-09-16 2006-03-22 刘粤荣 一种液体能量激发装置
CN101456642A (zh) * 2009-01-09 2009-06-17 廖怀德 一种高磁活化水的生产方法
CN101466643A (zh) * 2006-05-29 2009-06-24 株式会社志贺机能水研究所 水的电磁场处理方法及电磁场处理装置
CN201722187U (zh) * 2010-03-12 2011-01-26 郑士丰 创生水器结构
CN103058430A (zh) * 2012-12-18 2013-04-24 华南理工大学 一种高能小分子水的制备方法
CN104720058A (zh) * 2015-03-20 2015-06-24 欢乐海（北京）水业科技有限公司 多极微动能饮用水在制备用于通便的饮料、保健品或药物中的用途
CN104757673A (zh) * 2015-03-20 2015-07-08 欢乐海（北京）水业科技有限公司 多极微动能饮用水在制备用于降血压的饮料、保健品或药物中的用途
CN104757675A (zh) * 2015-03-20 2015-07-08 欢乐海（北京）水业科技有限公司 多极微动能饮用水在制备用于降血尿酸的饮料、保健品或药物中的用途
CN104757674A (zh) * 2015-03-20 2015-07-08 欢乐海（北京）水业科技有限公司 多极微动能饮用水在制备用于抗疲劳的饮料、保健品或药物中的用途
CN104757672A (zh) * 2015-03-20 2015-07-08 欢乐海（北京）水业科技有限公司 多极微动能饮用水在制备用于减肥的饮料、保健品或药物中的用途
CN104784207A (zh) * 2015-03-20 2015-07-22 欢乐海（北京）水业科技有限公司 多极微动能饮用水在制备用于降血尿素的饮料、保健品或药物中的用途
CN104803440A (zh) * 2015-03-20 2015-07-29 欢乐海（北京）水业科技有限公司 一种多极微动能饮用水及其制备方法和用途

Patent Citations (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02131186A (ja) * 1988-11-10 1990-05-18 Fuji Keiki:Kk 簡易型水処理装置
CN1086497A (zh) * 1993-06-17 1994-05-11 高汝云 一种水的灭菌、提高品质及祛除水垢的方法
CN2758237Y (zh) * 2004-05-31 2006-02-15 周林 频谱水装置
CN1748856A (zh) * 2004-09-16 2006-03-22 刘粤荣 一种液体能量激发装置
CN101466643A (zh) * 2006-05-29 2009-06-24 株式会社志贺机能水研究所 水的电磁场处理方法及电磁场处理装置
CN101456642A (zh) * 2009-01-09 2009-06-17 廖怀德 一种高磁活化水的生产方法
CN201722187U (zh) * 2010-03-12 2011-01-26 郑士丰 创生水器结构
CN103058430A (zh) * 2012-12-18 2013-04-24 华南理工大学 一种高能小分子水的制备方法
CN104720058A (zh) * 2015-03-20 2015-06-24 欢乐海（北京）水业科技有限公司 多极微动能饮用水在制备用于通便的饮料、保健品或药物中的用途
CN104757673A (zh) * 2015-03-20 2015-07-08 欢乐海（北京）水业科技有限公司 多极微动能饮用水在制备用于降血压的饮料、保健品或药物中的用途
CN104757675A (zh) * 2015-03-20 2015-07-08 欢乐海（北京）水业科技有限公司 多极微动能饮用水在制备用于降血尿酸的饮料、保健品或药物中的用途
CN104757674A (zh) * 2015-03-20 2015-07-08 欢乐海（北京）水业科技有限公司 多极微动能饮用水在制备用于抗疲劳的饮料、保健品或药物中的用途
CN104757672A (zh) * 2015-03-20 2015-07-08 欢乐海（北京）水业科技有限公司 多极微动能饮用水在制备用于减肥的饮料、保健品或药物中的用途
CN104784207A (zh) * 2015-03-20 2015-07-22 欢乐海（北京）水业科技有限公司 多极微动能饮用水在制备用于降血尿素的饮料、保健品或药物中的用途
CN104803440A (zh) * 2015-03-20 2015-07-29 欢乐海（北京）水业科技有限公司 一种多极微动能饮用水及其制备方法和用途

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ZHANG, JIANPING ET AL.: "Structural Change of Water Clusters and the Corresponding Biological Effects", CHEMISTRY, vol. 67, no. 4, 18 April 2004 (2004-04-18), ISSN: 0441-3776 *

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