WO2016117725A1 - Cartouche de diagnostic moléculaire - Google Patents

Cartouche de diagnostic moléculaire Download PDF

Info

Publication number
WO2016117725A1
WO2016117725A1 PCT/KR2015/000693 KR2015000693W WO2016117725A1 WO 2016117725 A1 WO2016117725 A1 WO 2016117725A1 KR 2015000693 W KR2015000693 W KR 2015000693W WO 2016117725 A1 WO2016117725 A1 WO 2016117725A1
Authority
WO
WIPO (PCT)
Prior art keywords
chamber
housing
reagent
molecular diagnostic
diagnostic cartridge
Prior art date
Application number
PCT/KR2015/000693
Other languages
English (en)
Inventor
Byeong Woo Bae
In Kyoung Hwang
Seong Min Park
Eun Ja Kim
Original Assignee
Infopia Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Infopia Co., Ltd. filed Critical Infopia Co., Ltd.
Priority to PCT/KR2015/000693 priority Critical patent/WO2016117725A1/fr
Publication of WO2016117725A1 publication Critical patent/WO2016117725A1/fr

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0644—Valves, specific forms thereof with moving parts rotary valves

Definitions

  • the present invention relates to a molecular diagnostic cartridge, and more particularly, to a molecular diagnostic cartridge which is selectively connected to a fluid according to movement of a lower housing.
  • a molecular diagnostic device is a device which is used to process a sample from a mixture, to measure the processed sample and thus to detect a disease, a virus or the like.
  • the molecular diagnostic device may be generally divided into a unit which processes the sample from the mixture, and a unit which detects the disease or the virus from the sample, and a series of operations thereof may be performed in one device.
  • Most of the molecular diagnostic devices use a cartridge which accommodates a sample or a reagent.
  • a conventional cartridge has a cylindrical shape, a flat shape or the like so as to perform a molecular diagnostic operation, and employs a method in which only a fluid path is changed to provide various fluid routes.
  • a means for changing the fluid path should be provided in the cartridge, it is difficult to provide the cartridge having an efficient structure and size due to restriction on a location thereof.
  • the present invention is directed to providing a molecular diagnostic cartridge which is selectively connected to a fluid according to movement of a lower housing.
  • One aspect of the present invention provides a molecular diagnostic cartridge including a first housing including processing chambers continuously connected to process a sample, a washing chamber for washing and a discharging chamber connected to the processing chambers and the washing chamber and having a discharging port through which a solution is discharged; and a second housing disposed under the first housing, and including a waste chamber configured to accommodate a waste solution and a target chamber configured to accommodate a target solution, and movably provided so that one of the waste chamber and the target chamber is selectively connected with the discharging port.
  • the cartridge since the fluid can be selectively connected according to the movement of the lower housing, the cartridge can have a small size.
  • FIG. 1 is a perspective view of a molecular diagnostic cartridge according to an exemplary embodiment of the present invention.
  • FIG. 2 is an exploded view of the molecular diagnostic cartridge according to the exemplary embodiment of the present invention.
  • FIG. 3 is a plan view of a cover part according to the exemplary embodiment of the present invention.
  • FIG. 4 is a plan view of a first housing according to the exemplary embodiment of the present invention.
  • FIG. 5 is a plan view of a second housing according to the exemplary embodiment of the present invention.
  • FIG. 6 is a cross-sectional view illustrating an operation of the molecular diagnostic cartridge according to the exemplary embodiment of the present invention.
  • FIG. 7 is a cross-sectional view illustrating another operation of the molecular diagnostic cartridge according to the exemplary embodiment of the present invention.
  • FIG. 8 is a plan view of a molecular diagnostic cartridge according to another exemplary embodiment of the present invention.
  • One aspect of the present invention provides a molecular diagnostic cartridge including a first housing including processing chambers continuously connected to process a sample, a washing chamber for washing and a discharging chamber connected to the processing chambers and the washing chamber and having a discharging port through which a solution is discharged; and a second housing disposed under the first housing, and including a waste chamber configured to accommodate a waste solution and a target chamber configured to accommodate a target solution, and movably provided so that one of the waste chamber and the target chamber is selectively connected with the discharging port.
  • the molecular diagnostic cartridge may include a first housing including processing a plurality of chambers continuously connected to process a sample, a washing chamber for washing and a discharging chamber connected to at least one of the plurality of processing chambers and the washing chamber and having a discharging port through which a solution is discharged; and a second housing disposed under the first housing, and including a waste chamber configured to accommodate a waste solution and a target chamber configured to accommodate a target solution, and movably provided so that one of the waste chamber and the target chamber is selectively connected with the discharging port.
  • a fluid path configured to connect the chambers of the first housing may be formed at the first housing, and the molecular diagnostic cartridge may further include a cover part provided on the first housing and included a pressure hole configured to receive a pressure to move a fluid through the fluid path.
  • a valve configured to allow movement of the fluid at a predetermined pressure or more may be provided in the fluid path.
  • a barrier configured to be broken at a predetermined pressure or more may be provided in the fluid path.
  • a fluid path configured to connect the chambers of the first housing may be formed at the first housing, and the molecular diagnostic cartridge further comprises a one-way valve configured to prevent a backflow of the fluid may be provided in the fluid path.
  • the adjacent chambers of the first housing may have bottom surfaces at different heights from each other.
  • the second housing may be configured so that one of the waste chamber and the target chamber is selectively connected with the discharging port by a rotational movement thereof.
  • the second housing may be configured so that one of the waste chamber and the target chamber is selectively connected with the discharging port by a sliding movement thereof.
  • the first housing may further include an elution chamber configured to accommodate a dissolving solution and connected to the discharging chamber.
  • the plurality of processing chambers may include a first chamber configured to accommodate a first reagent, a second chamber configured to receive and process the first reagent, a third chamber configured to accommodate a mixed solution including the sample, to receive the processed first reagent and to process the mixed solution, and a fourth chamber configured to accommodate a second reagent and to process the processed mixed solution with the second reagent, and the first chamber, the second chamber, the third chamber and the fourth chamber may be connected sequentially through a fluid path, and bottom surfaces thereof are at heights that are gradually lowered sequentially.
  • the plurality of processing chambers may further include a fifth chamber configured to accommodate a third reagent and connected to the fourth chamber, and the fifth chamber may have a higher height of a bottom surface than that of the bottom surface of the fourth chamber.
  • At least one of the plurality of processing chambers may include a stirring member configured to facilitate the sample processing.
  • the washing chamber may include a plurality of chambers of which of bottom surfaces are at heights that are gradually lowered in connection order.
  • At least a part of the target chamber may be formed of a transparent material.
  • the first housing may further include a reagent chamber configured to accommodate a reagent to be used in a reaction of the target solution, and connected to the target chamber by movement of the second housing.
  • the molecular diagnostic cartridge according to the present invention should be interpreted as a cartridge used in various measuring devices for detecting diseases, viruses, or the like, as well as a cartridge which accommodates a reagent used in the molecular diagnostic cartridge.
  • FIG. 1 is a perspective view of a molecular diagnostic cartridge 1000 according to an exemplary embodiment of the present invention.
  • the molecular diagnostic cartridge 1000 may include a cover part 1200, a first housing 1400 and a second housing 1600.
  • the cover part 1200 has a pressure hole 1220 through which a pressure necessary to move a fluid through a fluid path C is received
  • the first housing 1400 may include processing chambers 1410, a washing chamber 1430 and a discharging chamber 1420
  • the second housing 1600 may include a waste chamber 1620 and a target chamber 1640.
  • the cover part 1200 may be located on the first housing 1400, and may provide an upper surface of each chamber included in the first housing 1400.
  • the cover part 1200 may include at least one pressure hole 1220 and a sample inlet port 1240.
  • the pressure hole 1220 may receive the pressure necessary to move the fluid from the device in which the cartridge 1000 is installed, and the sample inlet part 1240 may be a passage through which a sample is received.
  • the pressure hole 1220 may be connected with a pressure providing means included in the device.
  • the device may have a pressure generating means for generating a pressure and a pressure transmitting means for transmitting the generated pressure to the cartridge, and the pressure hole 1220 may be connected with the pressure transmitting means to receive the pressure generated from the pressure generating means.
  • the pressure may be an air pressure.
  • the sample inlet port 1240 is a port through which the sample is introduced, and may pass through the cover part 1200 and may be connected with the chamber into which the sample will be introduced.
  • the sample may be blood, saliva, cells or the like.
  • a position of the sample inlet port 1240 may be changed according a position of the chamber into which the sample will be introduced.
  • cover part 1200 The detailed description of the cover part 1200 will be described with reference to FIG. 3.
  • the first housing 1400 may include the plurality of chambers and the fluid path C which connects the plurality of chambers.
  • the plurality of chambers may be the processing chambers 1410, the washing chamber 1430 and the discharging chamber 1420.
  • the second housing 1600 may include the waste chamber 1620 which accommodates the waste solution and the target chamber 1640 which accommodates the target solution to be measured, and may be moved to be selectively connected with a discharging port formed at the discharging chamber 1420.
  • FIG. 2 is an exploded view of the molecular diagnostic cartridge 1000 according to the exemplary embodiment of the present invention.
  • the molecular diagnostic cartridge 1000 may include the cover part 1200, the first housing 1400 and the second housing 1600, and the cover part 1200, the first housing 1400 and the second housing 1600 may be stacked sequentially.
  • the cover part 1200 may be located at the uppermost portion of the molecular diagnostic cartridge 1000, may be connected with the pressure generating means, and may provide a covering function which covers upper surfaces of the chambers included in the first housing 1400.
  • the first housing 1400 may be located under the cover 1200 to receive the pressure from the pressure hole 1220 and to receive the sample through the sample inlet port 1240.
  • the second housing 1600 may be coupled to a lower portion of the first housing 1400.
  • the second housing 1600 may be coupled to the first housing 1400 to be rotated or slid by an external force.
  • FIG. 3 is a plan view of the cover part 1200 according to the exemplary embodiment of the present invention.
  • the cover part 1200 may include the pressure hole 1220 and the sample inlet port 1240.
  • the pressure hole 1220 may be connected with the pressure transmitting means provided at the device so as to transmit the pressure to at least one of the chambers included in the first housing 1400.
  • the pressure hole 1220 may be provided to pass through the cover part 1200 and thus, to transmit the pressure received from the pressure transmitting means to the connected chamber.
  • the pressure hole 1220 may be formed in a circular shape, but is not limited thereto, and may have various shapes.
  • the pressure hole 1220 may be formed in a polygonal shape such as a triangular shape and a quadrangular shape or a modified circular shape such as an elliptical shape.
  • the pressure hole 1220 may be formed in a hole shape into which the pressure transmitting means is inserted and connected therewith, or may protrude so as to be inserted into the pressure transmitting means.
  • the pressure transmitting means When the pressure transmitting means is inserted into the pressure hole 1220 to provide the pressure, the pressure transmitting means may include a protruding portion which is inserted into the pressure hole 1220.
  • a diameter of the pressure hole 1220 may be provided larger than a diameter of the protruding portion of the pressure transmitting means, such that the protruding portion of the pressure transmitting means is inserted into the pressure hole 1220.
  • the pressure hole 1220 may be provided to have a sufficient area to insert the protruding portion of the pressure transmitting means therein.
  • a protruding portion of the pressure hole 1220 may be formed in a prismatic shape such as a triangular prism and a square pillar, or may be formed in a cylindrical shape.
  • the cover part 1200 may include a plurality of pressure holes 1220.
  • the plurality of pressure holes 1220 may be provided, and each of the plurality of the pressure holes 1220 may be located above each chamber to which the pressure is to be transmitted, and may be connected thereto.
  • the sample inlet port 1240 is a port through which the sample is introduced into a predetermined chamber, and may be provided to pass through the cover part 1200 located at a position corresponding to the predetermined chamber.
  • the sample inlet port 1240 may be open, when the sample is introduced, and then may be closed by a separate cap so as to prevent contamination from an outside, when the sample is not introduced.
  • FIG. 4 is a plan view of the first housing 1400 according to the exemplary embodiment of the present invention.
  • the first housing 1400 may include the processing chambers 1410, the washing chamber 1430, the discharging chamber 1420, an elution chamber 1440, a reagent buffer chamber 1451 and a reagent chamber 1452.
  • the processing chambers 1410 are chambers in which a process of processing the sample and extracting the target solution to be measured is performed, and a plurality of chambers may be provided according to a sample processing process.
  • the processing chambers 1410 may accommodate a reagent for processing the sample and may include a chamber for accommodating the sample.
  • the processing chambers 1410 may include a first chamber 1411, a second chamber 1412, a third chamber 1413 and a fourth chamber 1414. Also, the processing chambers 1410 may further a fifth chamber 1415.
  • the first chamber 1411 may be connected with the second chamber 1412 through the fluid path C, and a fluid accommodated in the first chamber 1411 may be moved to the second chamber 1412 through the fluid path C by the pressure supplied from the pressure hole 1220 to the first chamber 1411.
  • the fluid path which connects the first chamber 1411 and the second chamber 1412 may connect a lower end of a side surface of the first chamber 1411 and an upper end of a side surface of the second chamber 1412.
  • a bottom surface of the second chamber 1412 may be relatively located lower than that of the first chamber 1411.
  • the first chamber 1411 may be connected with the pressure hole 1220 to receive the pressure from the pressure hole 1220, and then may transmit the received pressure to the second chamber 1412.
  • the second chamber 1412 may be connected with the third chamber 1413 through the fluid path C, and the fluid accommodated therein may be moved to the third chamber 1413 through the fluid path C.
  • the fluid path C which connects the second chamber 1412 and the third chamber 1413 may connect a lower end of a side surface of the second chamber 1412 and an upper end of a side surface of the third chamber 1413.
  • a bottom surface of the third chamber 1413 may be relatively located lower than that of the second chamber 1412.
  • the second chamber 1412 may receive the pressure from the first chamber 1411, and then may transmit the received pressure to the third chamber 1413.
  • the third chamber 1413 may be the chamber for accommodating the sample, and may be connected with the sample inlet port 1240 to receive the sample.
  • the third chamber 1413 be connected with the fourth chamber 1414 through the fluid path C, and the fluid accommodated in the third chamber 1413 may be moved to the fourth chamber 1414 through the fluid path C by the pressure received from the second chamber 1412.
  • the fluid path C which connects the third chamber 1413 and the fourth chamber 1414 may connect a lower end of a side surface of the third chamber 1413 and an upper end of a side surface of the fourth chamber 1414.
  • a bottom surface of the fourth chamber 1414 may be relatively located lower than that of the third chamber 1413.
  • the fourth chamber 1414 may be connected with the fifth chamber 1415 and the discharging chamber 1420 through the fluid path C.
  • the fluid path which connects the fourth chamber 1414 and the fifth chamber 1415 may connect a lower end of a side surface of the fifth chamber 1415 and the upper end of the side surface of the fourth chamber 1414. Also, to allow the fluid to flow smoothly, the bottom surface of the fourth chamber 1414 may be relatively located lower than that of the fifth chamber 1415.
  • the fluid path which connects the fourth chamber 1414 and the discharging chamber 1420 may connect a lower end of a side surface of the fourth chamber 1414 and an upper end of the side surface of the discharging chamber 1420.
  • the fourth chamber 1414 may include a stirring member which facilitates the sample processing.
  • the stirring member is provided at an inner side of the fourth chamber 1414 to receive a rotating force from the device and thus to facilitate the sample processing performed in the fourth chamber 1414.
  • an impeller shaft may be provided as the stirring member to receive the rotating force from the device and to stir the sample.
  • the discharging chamber 1420 may be connected with the fourth chamber 1414, the washing chamber 1430 and the elution chamber 1440 through the fluid path C.
  • the fluid path which connects the discharging chamber 1420 and the washing chamber 1430 may connect a lower end of a side surface of the washing chamber 1430 and an upper end of a side surface of the discharging chamber 1420.
  • a bottom surface of the discharging chamber 1420 may be relatively located lower than that of the washing chamber 1430.
  • the fluid path which connects the discharging chamber 1420 and the elution chamber 1440 may connect a lower end of a side surface of the elution chamber 1440 and the upper end of the side surface of the discharging chamber 1420. Also, to allow the fluid to flow smoothly, the bottom surface of the discharging chamber 1420 may be relatively located lower than that of the elution chamber 1440.
  • the discharging port through which the fluid is discharged may be formed at a lower portion of the discharging chamber 1420.
  • the discharging port may be a passage through which the fluid accommodated in the discharging chamber 1420 is discharged to an outside of the first housing 1400.
  • the discharging chamber 1420 may include a filter which temporarily collects a target object.
  • the discharging chamber 1420 may include a membrane filter which collects DNA.
  • the filter may be located at the lower portion of the discharging chamber 1420, and may be located at an upper portion of the discharging port to collect the target object from the fluid discharged through the discharging port.
  • the first housing 140 included only one washing chamber 1430.
  • the first housing 1400 may include a plurality of washing chambers 1430.
  • the washing chambers 1430 may be connected with each other through the fluid path C, and one of the plurality of washing chambers 1430 may be connected with the discharging chamber 1420.
  • the first housing 1400 may include a first washing chamber 1431, a second washing chamber 1432 and a third washing chamber 1433, and the first washing chamber 1431, the second washing chamber 1432 and the third washing chamber 1433 may be connected with each other through the fluid path C.
  • the first washing chamber 1431, the second washing chamber 1432 and the third washing chamber 1433 may be formed to have bottom surfaces at different heights from each other.
  • the bottom surfaces of the plurality of washing chambers 1430 may be formed to be gradually lower in order of the first washing chamber 1431, the second washing chamber 1432 and the third washing chamber 1433.
  • the reagent chamber 1452 may be connected with the reagent buffer chamber 1451 through the fluid path.
  • the fluid path which connects the reagent chamber 1452 and the reagent buffer chamber 1451 may connect a lower end of a side surface of the reagent buffer chamber 1451 and an upper end of a side surface of the reagent chamber 1452. Also, to allow the fluid to flow smoothly, a bottom surface of the reagent chamber 1452 may be relatively located lower than that of the reagent buffer chamber 1451.
  • the fluid path C which discharges the fluid to the outside of the first housing 1400 may be formed at a lower portion of the reagent chamber 1452 to discharge the fluid accommodated in the reagent chamber 1452 to the outside of the first housing 1400.
  • the fluid path C may serve to connect the chambers of the first housing 1400 or to provide a passage through which the pressure is transmitted.
  • a control member which controls the fluid or the pressure may be provided in the fluid path C.
  • the control member may be a one-way valve which prevents a backflow of the fluid, may be a valve which allows movement of the fluid at a predetermined pressure or more, or may be a barrier which is broken at the predetermined pressure or more.
  • the control member may be provided at each fluid path C, and may be selectively provided in the fluid path C which is required to be controlled. Further, the control member may be provided at an end of the fluid path C to be substantially in contact with an inner surface of the chamber.
  • FIG. 5 is a plan view of the second housing 1600 according to the exemplary embodiment of the present invention.
  • the second housing 1600 may include the waste chamber 1620 and the target chamber 1640.
  • the waste chamber 1620 may be selectively connected with the discharging port of the first housing 1400 by movement of the second housing 1600.
  • the target chamber 1640 may be selectively connected with the discharging port of the first housing 1400 and the fluid path C provided under the reagent chamber 1452 by the movement of the second housing 1600.
  • one of the waste chamber 1620 and the target chamber 1640 may be connected with the discharging port of the first housing 1400 by the movement of the second housing 1600.
  • the waste chamber 1620 and the target chamber 1640 may be provided to be separated from each other, such that the fluid is not moved to each other, and at least a part of the target chamber 1640 may be formed of a transparent material.
  • FIG. 6 is a cross-sectional view illustrating an operation of the molecular diagnostic cartridge 1000 according to the exemplary embodiment of the present invention.
  • FIG. 6 is a cross-sectional view taken along a line A-A’ of FIG. 4.
  • the cover part 1200 may include the pressure hole 1220 and the sample inlet port 1240
  • the first housing 1400 may include the first chamber 1411, the second chamber 1412, the third chamber 1413, the fourth chamber 1414, the fifth chamber 1415, the discharging chamber 1420, the first washing chamber 1431, the second washing chamber 1432 and the third washing chamber 1433
  • the second housing 1600 may include the waste chamber 1620 and the target chamber 1640.
  • the first chamber 1411 may be a chamber which accommodates a first reagent, and may be a place in which an initial pressure is provided and movement of the fluid is started.
  • the first reagent may be a proteinase K (PK) buffer which dissolves a proteinase K (PK) reagent.
  • the first chamber 1411 may be connected with the second chamber 1412 through the fluid path C, and the first reagent accommodated in the first chamber 1411 may be moved to the second chamber 1412 through the fluid path C by the pressure received from the pressure hole 1220 to the first chamber 1411.
  • the first chamber 1411 is connected with the pressure hole 1220 to receive the pressure from the pressure hole 1220, and to transmit the received pressure to the second chamber 1412.
  • the second chamber 1412 may accommodate the PK reagent which dissolves the sample, and the first reagent may be introduced therein from the first chamber.
  • the first reagent introduced from the second chamber 1412 is mixed with the PK reagent accommodated in the second chamber 1412, and the PK reagent and the first reagent may be moved to the third chamber 1413 through the fluid path C by the pressure received from the first chamber 1411.
  • the second chamber 1412 may receive the pressure from the first chamber 1411, and may transmit the received pressure to the third chamber 1413.
  • the third chamber 1413 may a chamber which accommodates the sample, and may be connected with the sample inlet port 1240 to receive the sample.
  • the third chamber 1413 may receive the first reagent and the PK reagent through the second chamber 1412, and a mixed solution in which the first reagent, the PK reagent and the sample are mixed may be generated.
  • the third chamber 1413 may be connected with the fourth chamber 1414 through the fluid path C, and the mixed solution may be moved to the fourth chamber 1414 through the fluid path C by the pressure received from the second chamber 1412.
  • the fourth chamber 1414 may be a chamber which accommodates a second reagent and into which the mixed solution of the first reagent, the PK reagent and the sample is introduced and treated by the second reagent.
  • the second reagent may be various well-known dissolving reagents which dissolve the sample.
  • the second reagent may include a buffer salt such as guanidinium salt.
  • the second reagent may include a chaotropic agent such as guanidinium thiocyanate, a chelating agent such as ethylenediaminetetraacetic acid (EDTA), and a buffer salt such as tris(hidroxymethyl)aminomethane(Tris-HCl).
  • the second reagent may include a non-ionic surfactant.
  • a non-ionic surfactant preferably Triton X-100, Tween (an ethylene oxide adduct of a sorbitan ester), or 2-mercaptoethanol, most preferably Triton X-100 may be used as the non-ionic surfactant.
  • the second reagent may have a non-acidic property, e.g., a neutral property or an alkaline property.
  • the mixed solution of the first reagent, the PK reagent and the sample may react with the second reagent, and a dissolving reaction which dissolves the sample to extract the target object from the sample mixed in the mixed solution may be performed.
  • a third reagent may be introduced from the fifth chamber 1415 by the pressure received from the pressure hole 1220 into the fifth chamber 1415.
  • the target object may be DNA, RNA or nucleic acid.
  • the third reagent may be a reagent which separates the target object from the sample, and a separation reaction in which the target object is separated from the sample according to the introduction of the third reagent may be performed.
  • a substantial sample processing may be performed in the fourth chamber 1414, and the target object and other fluids may be provided as sample processed products.
  • the stirring member included in the fourth chamber 1414 may be operated by the device.
  • the target object and the other fluids generated when the sample processing is finished through the dissolving reaction and the separation reaction in the fourth chamber 1414 may be introduced into the discharging chamber 1420.
  • the target object and the other fluids introduced into the discharging chamber 1420 may pass through the filter provided in the discharging chamber 1420, and then may be discharged to the outside of the first housing. At this time, the target object may not pass through the filter, and may be collected by the filter, and only the other fluids may pass through the filter.
  • the discharging port may be connected with the waste chamber 1620 of the second housing 1600, and the discharged other fluids may be accommodated in the waste chamber 1620.
  • the discharging port and the waste chamber 1620 may be connected with each other in advance. However, in the case in which the discharging port and the waste chamber 1620 are not connected with each other, the discharging port and the waste chamber 1620 may be connected with each other by a rotation of the second housing 1600.
  • a washing solution may be introduced from the washing chamber 1430 into the discharging chamber 1420.
  • the washing solution is a solution which removes impurities mixed with the collected target object, and a component thereof is not limited, as long as the component may selectively wash and remove the impurities other than the nucleic acid.
  • the washing solution may include ethanol of 90 to 100 % strength, isopropanol or the like.
  • the washing solution introduced from the discharging chamber 1420 may be accommodated with the impurities in the waste chamber 1620 through the discharging port. Therefore, the waste solution including the other fluids, the impurities and the washing solution may be accommodated in the waste chamber 1620.
  • FIG. 7 is a cross-sectional view illustrating another operation of the molecular diagnostic cartridge 1000 according to the exemplary embodiment of the present invention.
  • FIG. 7 is a cross-sectional view taken along a line B-B’ of FIG. 4.
  • the first housing 1400 may include the discharging chamber 1420, the elution chamber 1440, the reagent chamber 1452 and the reagent buffer chamber 1451, and the elution chamber 1440 and the reagent buffer chamber 1451 may be connected with the pressure hole 1220. Also, the discharging port connected with the discharging chamber 1420 and the fluid path C connected with the reagent chamber 1452 may be connected with the target chamber 1640.
  • the second housing is rotated by the device, and the discharging port and the target chamber 1640 may be connected with each other. At this time, the discharging port and the waste chamber 1620 may be separated from each other, and thus may not be connected with each other.
  • the dissolving solution introduced into the discharging chamber 1420 may dissolve the target object collected by the filter and may separate the target object from the filter.
  • a target solution in which the dissolving solution and the target object separated from the filter are mixed may be accommodated into the target chamber 1640 through the discharging port.
  • a reagent may be introduced into the target chamber 1640.
  • the reagent may be introduced from the reagent chamber 1452.
  • a reagent buffer may be introduced from the reagent buffer chamber 1451 into the reagent chamber 1452, and may be mixed with the reagent.
  • the reagent buffer may be introduced into the reagent chamber 1452 by the pressure provided to the reagent buffer chamber 1451.
  • the reagent mixed with the reagent buffer may be moved to the target chamber 1640 by the pressure obtained by transmitting the pressure provided to the reagent buffer chamber 1451 to the reagent chamber 1452.
  • the second housing 1600 is rotated by the device, and the discharging port and the target chamber 1640 are connected with each other at the same time.
  • the discharging port and the target chamber 1640 may be connected with each other by the rotation of the second housing 1600, and then may be connected with the fluid path C connected with the reagent chamber 1452 by another rotation of the second housing 1600.
  • the waste chamber 1620 and the target chamber 1640 were selectively connected with the discharging port by the rotation of the second housing 1600.
  • the waste chamber 1620 and the target chamber 1640 may be selectively connected with the discharging port by a sliding movement of the second housing 1600.
  • the sample processing and washing process is the same as each other, and there is a difference in a structure in which the waste chamber 1620 and the target chamber 1640 are selectively connected with the discharging port.
  • FIG. 8 is a plan view of the molecular diagnostic cartridge 1000 according to another exemplary embodiment of the present invention.
  • the second housing 1600 may be slid by the device, and the discharging port and the target chamber 1640 may be connected with each other. At this time, the discharging port and the waste chamber 1620 may be separated from each other and may not be connected with each other.
  • the sliding movement may mean that the second housing 1600 is moved on a plane located under the first housing 1400, and the second housing 1600 and the first housing 1400 may not be separated from each other and may be moved while parts thereof are coupled with each other.
  • the dissolving solution introduced into the discharging chamber 1420 may dissolve the target object collected by the filter and then may separate the target object from the filter, and the target solution in which the dissolving solution and the target object separated from the filter are mixed may be accommodated into the target chamber 1640 through the discharging port.
  • the target chamber 1640 and the fluid path provided at the reagent chamber 1452 may be connected with each other by the sliding movement of the second housing 1600.
  • the reagent may be introduced into the target chamber 1640.
  • the reagent may be from the reagent chamber 1452.
  • the reagent buffer may be introduced from the reagent buffer chamber 1451 into the reagent chamber 1452, and may be mixed with the reagent.
  • the reagent buffer may be introduced into the reagent chamber 1452 by the pressure provided to the reagent buffer chamber 1451.
  • the reagent mixed with the reagent buffer may be moved to the target chamber 1640 by the pressure obtained by transmitting the pressure provided to the reagent buffer chamber 1451 to the reagent chamber 1452.

Abstract

L'invention concerne une cartouche de diagnostic moléculaire. La cartouche de diagnostic moléculaire selon un aspect de la présente invention comprend un premier boîtier comprenant une pluralité de chambres de traitement raccordées de façon continue pour traiter un échantillon, une chambre de lavage pour le lavage et une chambre d'évacuation raccordée à au moins une chambre de traitement parmi la pluralité de chambres de traitement et la chambre de lavage et ayant un orifice d'évacuation par lequel une solution est évacuée ; et un second boîtier disposé sous le premier boîtier et comprenant une chambre de rejet conçue pour recevoir une solution de rejet et une chambre de cible conçue pour recevoir une solution cible et disposée de manière mobile de sorte que l'une de la chambre de rejet et la chambre de cible est raccordée sélectivement à l'orifice d'évacuation.
PCT/KR2015/000693 2015-01-23 2015-01-23 Cartouche de diagnostic moléculaire WO2016117725A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/KR2015/000693 WO2016117725A1 (fr) 2015-01-23 2015-01-23 Cartouche de diagnostic moléculaire

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/KR2015/000693 WO2016117725A1 (fr) 2015-01-23 2015-01-23 Cartouche de diagnostic moléculaire

Publications (1)

Publication Number Publication Date
WO2016117725A1 true WO2016117725A1 (fr) 2016-07-28

Family

ID=56417250

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2015/000693 WO2016117725A1 (fr) 2015-01-23 2015-01-23 Cartouche de diagnostic moléculaire

Country Status (1)

Country Link
WO (1) WO2016117725A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6729196B2 (en) * 1999-03-10 2004-05-04 Mesosystems Technology, Inc. Biological individual sampler
US20110275058A1 (en) * 2010-02-23 2011-11-10 Rheonix, Inc. Self-contained biological assay apparatus, methods, and applications
US20120329142A1 (en) * 2006-03-15 2012-12-27 Micronics, Inc. Integrated nucleic acid assays
US8562918B2 (en) * 2009-06-05 2013-10-22 Integenx Inc. Universal sample preparation system and use in an integrated analysis system
US8894946B2 (en) * 2011-10-21 2014-11-25 Integenx Inc. Sample preparation, processing and analysis systems

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6729196B2 (en) * 1999-03-10 2004-05-04 Mesosystems Technology, Inc. Biological individual sampler
US20120329142A1 (en) * 2006-03-15 2012-12-27 Micronics, Inc. Integrated nucleic acid assays
US8562918B2 (en) * 2009-06-05 2013-10-22 Integenx Inc. Universal sample preparation system and use in an integrated analysis system
US20110275058A1 (en) * 2010-02-23 2011-11-10 Rheonix, Inc. Self-contained biological assay apparatus, methods, and applications
US8894946B2 (en) * 2011-10-21 2014-11-25 Integenx Inc. Sample preparation, processing and analysis systems

Similar Documents

Publication Publication Date Title
WO2014058251A1 (fr) Appareil de traitement d'échantillon et appareil d'analyse automatique comprenant celui-ci
WO2011112023A2 (fr) Puce pour la séparation de cellules sanguines
WO2009125971A2 (fr) Appareil de raffinage automatique, ensemble plaque à puits multiples et procédé d'extraction de l'hexane d'échantillons biologiques
WO2023000654A1 (fr) Kit de test génétique et appareil de test génétique
WO2015130001A1 (fr) Système d'épuration d'air et procédé de commande de celui-ci
WO2016167515A1 (fr) Dispositif de traitement d'étalement d'échantillon liquide
WO2015060664A1 (fr) Appareil de surveillance à multiples orifices d'échantillonnage pour mesurer le niveau de pollution et procédé de surveillance l'utilisant
WO2018124462A1 (fr) Unité de chambre tampon pour équipement de traitement de tranche
WO2009151198A1 (fr) Analyseur chimique par lots
EP3111198A1 (fr) Appareil et procédé de mesure de microbes aériens
WO2020111462A1 (fr) Micro-dispositif intégré pour analyse génétique rotative
WO2016208889A2 (fr) Purificateur d'air
WO2020036435A1 (fr) Ensemble tube destiné à goutter sur une bande de diagnostic et à extraire un fragment génétique
WO2016117725A1 (fr) Cartouche de diagnostic moléculaire
WO2019039911A9 (fr) Puce d'analyse d'échantillon, dispositif d'analyse d'échantillon contenant celle-ci et cartouche montée sur une puce d'analyse d'échantillon
WO2020060080A1 (fr) Système automatisé d'extraction d'acide nucléique
EP3077829A1 (fr) Appareil de test de biomatériau et son procédé de commande
WO2012086994A2 (fr) Système microfluidique
WO2023018109A1 (fr) Système de traitement de fluide et procédé de centrifugation l'utilisant
WO2023018193A1 (fr) Appareil d'extraction d'acide nucléique dans lequel des réactifs sont distribués en vrac
WO2011055873A1 (fr) Appareil pour extraire un materiau biochimique d'un echantillon biologique
WO2016208891A1 (fr) Purificateur d'air
WO2017204512A1 (fr) Module pcr
WO2013118990A1 (fr) Appareil d'extraction d'acides nucléiques à ultra-haute vitesse et procédé d'extraction d'acides nucléiques l'utilisant
WO2015129965A1 (fr) Kit de centrifugation

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 15878980

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 15878980

Country of ref document: EP

Kind code of ref document: A1