WO2015129965A1 - Kit de centrifugation - Google Patents

Kit de centrifugation Download PDF

Info

Publication number
WO2015129965A1
WO2015129965A1 PCT/KR2014/005156 KR2014005156W WO2015129965A1 WO 2015129965 A1 WO2015129965 A1 WO 2015129965A1 KR 2014005156 W KR2014005156 W KR 2014005156W WO 2015129965 A1 WO2015129965 A1 WO 2015129965A1
Authority
WO
WIPO (PCT)
Prior art keywords
mixed solution
centrifugation
container
outlet
chamber
Prior art date
Application number
PCT/KR2014/005156
Other languages
English (en)
Korean (ko)
Inventor
장기영
Original Assignee
(주)어핀텍
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by (주)어핀텍 filed Critical (주)어핀텍
Publication of WO2015129965A1 publication Critical patent/WO2015129965A1/fr

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/08—Rotary bowls
    • B04B7/12—Inserts, e.g. armouring plates
    • B04B7/16—Sieves or filters
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/02—Centrifuges consisting of a plurality of separate bowls rotating round an axis situated between the bowls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes

Definitions

  • the present invention relates to a centrifugation kit, and more particularly, to a centrifugation kit that can easily extract or collect a desired final material through centrifugation by mixing a sample having a biological or chemical substance such as nucleic acid with a buffer. will be.
  • a centrifugation kit in general, includes a mixture containing a sample and a buffer for centrifugation in a state in which a spin column and a collection tube are combined, and the spin column is integrated with the spin column.
  • the vessel-coupled centrifuge kit is loaded into a rotor in the centrifuge so that the desired material in the mixed solution contained in the spin column by the centrifugal force of the centrifuge remains in the filter below the spin column and the remaining material and buffers are out of the spin column. Spilled and collected in an integrated container.
  • centrifugation kits of this kind require pretreatment before mixed materials such as samples and buffers are injected into the spin column.
  • lysis buffer and proteinase K are released to release DNA or RNA trapped in the cell wall.
  • lysis buffer and proteinase K are released to release DNA or RNA trapped in the cell wall.
  • the rotor used generally has an inlet of a 45 ° spin column assembly so that the liquid in the spin column or the centrifugal force after the centrifugal force does not flow down after the centrifugal force is applied, and the angle is used to prevent the liquid from flowing down.
  • Swing bucket rotor with angle rotor or swing bucket with spin column and integrated container to position the spin column and integrated container in the horizontal direction when centrifugal force is applied and to the vertical direction when there is no centrifugal force. (swing bucket rotor) is used.
  • the present inventors have developed a centrifugal kit having a pre-processing container which is directly connected to a spin column and directly inserted into a rotor of a centrifuge or a filter unit inserted with a pre-processing container. Reached.
  • the present invention has been made to solve the above problems, by providing a pre-treatment container or a pre-treatment container is inserted into the filter column is inserted into the rotor of the centrifuge directly connected to the spin column, the biological or chemical material for centrifugation It is an object of the present invention to provide a centrifugal separation kit that facilitates injection of a sample and a buffer, improves workability, and facilitates automation.
  • the present invention provides a chamber containing a mixed solution containing a sample and a buffer having a biological or chemical substance for centrifugation, an injection unit into which the sample and the buffer are injected into the chamber, and a centrifugation.
  • a pretreatment container having an outlet portion through which the mixed solution flows out of the chamber by a centrifugal force generated at the time;
  • An inlet part through which the mixed solution flowing out through the outlet part flows in, a mixed solution accommodating part accommodating the mixed solution introduced from the inlet part, and a discharge part discharging the centrifuged solution centrifuged from the mixed solution; It is provided between the inlet and the outlet provides a centrifugation kit comprising a spin column having a filter unit for filtering the mixed solution is centrifuged.
  • the apparatus may further include an integrated container having a centrifugal liquid accommodating part configured to receive the centrifugal liquid discharged through the filter part by being centrifuged from the spin column.
  • the outlet of the pretreatment container is characterized in that it is screwed, or fitted, or loosely inserted into the inlet of the spin column.
  • the accumulation container is located adjacent to the discharge portion, characterized in that provided inclined with respect to the axis of the discharge portion.
  • the object is a chamber containing a mixed solution containing a sample and a buffer having a biological or chemical substance for centrifugation, an injection unit into which the sample and the buffer is injected into the chamber, and the inlet and 45 °
  • a pretreatment container positioned at an angle of ⁇ 135 ° and having an outlet portion through which the mixed solution in the chamber is centrifuged by centrifugal force generated during centrifugation;
  • a filter unit provided at an outlet of the pretreatment container and filtering the mixed solution to be centrifuged; It is also achieved by a centrifugation kit comprising an integrated container formed with a centrifugation liquid receiving portion for receiving the centrifugal liquid discharged through the filter portion.
  • the integrated container is located adjacent to the outlet portion, characterized in that provided inclined with respect to the axis of the outlet portion.
  • the pretreatment container has four cylindrical side wall portions, an upper plate portion and a lower plate portion for blocking openings at both ends formed by the four side wall portions, and the injection portion does not leak the mixed solution in the chamber by centrifugal force generated during centrifugation. It is provided on the upper plate portion, and the outlet portion is characterized in that it is provided on the upper side of any one of the four side wall portion so that the mixed solution in the chamber smoothly flows out by the centrifugal force generated during centrifugation.
  • the injection portion and the outlet portion is characterized in that the angle of 45 ° to 135 °.
  • the width of the pretreatment container is increased from the injection portion toward the outlet portion.
  • the filter unit may be formed of a membrane or a media filter.
  • the pretreatment container is characterized in that it comprises a jaw to prevent the mixed solution contained in the chamber to flow out to the injection portion.
  • the present invention it is easy to inject the sample and the buffer by injecting the sample and the buffer having the biological or chemical substance for centrifugation through the pretreatment container provided at the front end of the spin column without directly injecting the sample into the spin column. It can improve the performance and facilitate the automation.
  • the filter unit may be embedded inside the outlet of the pretreatment container to simultaneously perform the roles of the pretreatment container and the spin column.
  • FIG. 1 is a perspective view of a centrifugal separation kit according to a first embodiment of the present invention
  • FIG. 2 is a cross-sectional view of FIG.
  • FIG. 3 is a perspective view of the pretreatment container of FIG.
  • FIG. 4 is a cross-sectional view of a centrifugal separation kit according to a second embodiment of the present invention.
  • FIG. 5 is a perspective view of the pretreatment container of FIG. 4, FIG.
  • FIG. 6 is a plan view of the rotor of the centrifuge in which the centrifugation kit according to the first and second embodiments of the present invention is used;
  • FIG. 7 is a longitudinal sectional view of FIG. 6;
  • FIG. 8 is a plan view showing a state in which the centrifuge kit according to the first and second embodiments of the present invention is mounted on the rotor of the centrifuge of FIG. 6;
  • FIG. 9 is a longitudinal sectional view of FIG. 8;
  • FIG. 10 is a cross-sectional view showing the centrifugation kit mounted on the rotor of the centrifuge according to the first and second embodiments of the present invention and centrifuged;
  • FIG. 11 is a perspective view of a centrifugal separation kit according to a third embodiment of the present invention.
  • FIG. 12 is a cross-sectional view of FIG. 11,
  • Figure 13 is a longitudinal cross-sectional view showing a state in which the centrifuge kit according to the third embodiment of the present invention is mounted on the rotor of the centrifuge,
  • FIG. 14 is a perspective view of a centrifugation kit according to a fourth embodiment of the present invention.
  • FIG. 15 is a perspective view of a centrifugal separation kit according to a fifth embodiment of the present invention.
  • FIG. 16 is a cross-sectional view of FIG. 15 schematically illustrating an outflow state of a mixed solution in a pretreatment container during centrifugation;
  • FIG. 17 is a perspective view of the pretreatment container of FIG. 15;
  • FIG. 18 is a front view of the pretreatment container of FIG. 15.
  • FIG. 1 and 2 illustrate a centrifugation kit according to a first embodiment of the present invention.
  • the centrifugation kit 10a includes a pretreatment container 11a and a spin column 31a.
  • the pre-processing container 11a is formed by four cylindrical side wall portions 13a, 13b, 13c, and 13d and four side wall portions 13a, 13b, 13c, and 13d.
  • a rectangular cylindrical shape having an upper plate portion 15 and a lower plate portion 17 for blocking openings formed at both ends is formed, and includes a chamber 21, an injection portion 23, and an outlet portion 25.
  • the chamber 21 is provided inside the pretreatment container 11a to accommodate a sample having a biological or chemical substance for centrifugation and a mixed solution 3 containing a buffer, and the mixture contained in the chamber 21 during centrifugation.
  • the solution 3 is centrifuged by centrifugal force.
  • the injection part 23 is provided in the upper plate part 15, and the sample and the buffer are injected into the chamber 21 through the injection part 23.
  • the injection part 23 is provided in the upper plate part 15 so that the mixed solution 3 in the chamber 21 does not flow out by the centrifugal force generated during centrifugation.
  • the outlet portion 25 is provided in the side wall portion 13a of any one of the four side wall portions 13a, 13b, 13c, and 13d, and the mixed solution 3 in the chamber 21 is caused by centrifugal force generated during centrifugation. Through the outlet part 25, it flows out of the pretreatment container 11a, ie toward the spin column 31a.
  • the outlet part 25 is provided above the side wall part 13a so that the mixed solution 3 in the chamber 21 does not flow out through the outlet part 25 when it is not centrifuged.
  • the outlet portion 25 has a stepped outer circumference of the outlet portion 25 so that the spin column 31a is fitted. Then, the outlet portion 25 forms a right angle with the injection portion 23.
  • the outlet portion 25 and the injection portion 23 may be provided at an angle of 45 ° to 135 °.
  • the pretreatment container 11a has a shape in which the width of the pretreatment container 11a increases from the injection portion 23 toward the outlet 25. This makes it possible to mount as many pretreatment containers 11a as possible in the rotor 101 (see FIG. 6) of the centrifuge.
  • the spin column 31a has a hollow circular tube shape of which both sides are open, and includes a mixed solution containing part 33, an inlet part 35, an outlet part 43, and a filter part 45.
  • the mixed solution accommodating part 33 is formed inside the spin column 31a and accommodates the mixed solution 3 introduced from the inlet part 35.
  • the mixed solution 3 contained in the mixed solution containing portion 33 is centrifuged by the centrifugal force generated during centrifugation.
  • the inlet part 35 is formed at an opening at one end of the spin column 31a to be fitted to the outlet part 25 of the pretreatment container 11a and to be mixed through the outlet part 25 of the pretreatment container 11a.
  • the solution 3 flows into the mixed solution containing portion 33 through the inlet portion 35.
  • the inlet portion 35 is equipped with a cap 39, the cap 39 is connected to the spin column 31a by a connecting string 41.
  • the cap 39 and the connecting strap 41 may be selectively interposed.
  • the discharge part 43 is formed at the other end of the spin column 31a, and the centrifugal liquid 5 (see FIG. 10) centrifuged from the mixed solution 3 is passed through the spin part 31a. ) Is discharged outward, that is, toward the accumulation vessel 51.
  • the discharge part 43 forms a step with the mixed solution accommodating part 33 and has a diameter relatively smaller than that of the injection part 23.
  • a filter portion 45 is provided at the lower end of the mixed solution containing portion 33 of the spin column 31a, that is, between the inlet portion 35 and the discharge portion 43 to filter the mixed solution 3 to be centrifuged. .
  • the filter part 45 selectively filters the mixed solution 3 discharged through the discharge part 43, that is, filters or passes a desired substance from the mixed solution 3 containing the sample and the buffer. do.
  • the filter part 45 may be formed of a membrane or a media filter. Common membrane components include silica, cellulose, nylon, and PTFE (polytetrafluorethylene). Exchange-exchange resins.
  • the centrifugation kit 10a according to the present invention further includes an integration container 51.
  • the accumulation container 51 has a predetermined length and has a hollow circular tube shape with one side opened. Inside the integrated container 51, a centrifugal liquid accommodating part 53 is formed which receives the centrifugal liquid 5 which is centrifuged from the spin column 31a and discharged through the filter part 45.
  • the accumulation vessel 51 is located adjacent to the discharge portion 43 of the spin column 31a and is provided to be inclined downward with respect to the axis of the discharge portion 43.
  • the integrated container 51 may be optionally interposed.
  • Figure 6 and Figure 7 shows the rotor of the centrifuge is centrifuged with a centrifuge kit according to the present invention.
  • the rotor 101 of the centrifuge has a disk shape, and a central hole 103 that can be coupled to a rotating shaft (not shown) of the motor is formed in the center, and the central hole 103 is formed.
  • the plurality of container seating portions 105, the plurality of column seating portions 107, and the plurality of container seating portions 111 are provided at equal intervals along the circumference of the central hole 103.
  • the pretreatment container 11a is seated on the container seating portion 105 so that the injection portion 23 of the pretreatment container 11a is positioned adjacent to the central hole 103.
  • a column support 109 on which the spin column 31a is supported is provided on the column seat 107 of the rotor 101 so that the spin column 31a connected to the pretreatment container 11a is horizontal.
  • the container support 113 is provided in the container mounting part 111 of the rotor 101 so that the integration container 51 may be inclined with respect to the axis of the spin column 31a and the discharge part 43. As shown in FIG.
  • a pair of pretreatment containers 11a, a pair of spin columns 31a, and a pair of integration vessels 51 are symmetrical and centrifuge The method of centrifuging in the state mounted on the rotor 101 is demonstrated.
  • the pretreatment container 11a, the spin column 31a, and the integrated container 51 are seated on the container seating portion 105, the column seating portion 107, and the container seating portion 111 of the rotor 101.
  • the spin column 31a is fitted to the outlet portion 25 of the pretreatment container 11a to connect the pretreatment container 11a and the spin column 31a.
  • a sample and a buffer having a biological or chemical substance such as nucleic acid for centrifugation are injected into the chamber 21 through the injection section 23 of the pretreatment container 11a, respectively.
  • the injection portion 23 of the pretreatment container 11a faces upward of the rotor 101, so that the sample and the buffer can be easily injected into the pretreatment container 11a using a pipette.
  • the mixed solution 3 including the sample and the buffer injected into the chamber 21 does not flow out through the outlet 25.
  • the rotor 101 of the centrifuge is rotated in one direction.
  • the mixed solution 3 contained in the pretreatment container 11a flows out through the outlet 25 by centrifugal force, and spins through the inlet 35 of the spin column 31a. It is accommodated in the mixed solution container 33 of the column 31a.
  • the mixed solution 3 contained in the mixed solution containing part 33 of the spin column 31a is centrifuged while being filtered through the filter part 45 by centrifugal force, followed by centrifuged centrifugal solution 5. Is discharged toward the integrated container 51 through the discharge portion 43 of the spin column 31a.
  • the male screw 27 is formed on the outer circumference of the outlet portion 25 of the pretreatment container 11b, the inlet portion of the spin column 31b ( A centrifugal separation kit 10b according to the second embodiment of the present invention is shown in which an internal thread 37 is formed at an inner circumference of 35 so that the pretreatment container 11b and the spin column 31b are screwed together.
  • the spin columns 31a and 31b are not directly injected into the spin columns 31a and 31b without the sample and the buffer having the biological or chemical substance for centrifugation.
  • the sample and the buffer can be easily injected, workability can be improved, and automation can be facilitated.
  • the outlet portion 25 of the pretreatment containers 11a and 11b and the inlet portion 35 of the spin columns 31a and 31b are screwed or fitted to the pretreatment containers 11a and 11b.
  • the spin columns 31a and 31b are described as being connected, although not shown, the outlet of the pretreatment container is loosely inserted into the inlet of the spin column, so that the mixed solution in the pretreatment container may flow into the spin column.
  • FIGS. 11 and 12 illustrate a centrifugal separation kit according to a third embodiment of the present invention.
  • the centrifugation kit 10c according to the third embodiment of the present invention unlike the above-described embodiments, does not have a spin column interposed between the pretreatment container 11c and the integration vessel 51. not.
  • the centrifugal separation kit 10c includes a pretreatment container 11c, a filter part 45, and an integration container 51.
  • the pretreatment container 11c of the centrifugal separation kit 10c according to the third embodiment of the present invention has the same shape and structure as the pretreatment container 11a according to the first embodiment described above. Omit the description. Meanwhile, the pretreatment container 11c of the centrifuge kit 10c according to the third embodiment of the present invention serves to centrifuge the mixed solution 3 in the chamber 21 by centrifugal force generated during centrifugation.
  • the filter part 45 is provided in the outflow part 25 of the pretreatment container 11c, and filters the mixed solution centrifuged.
  • the integrated container 51 has a hollow circular tube shape with one side opened, and the centrifugal liquid 5 centrifuged from the pretreatment container 11a inside the integrated container 51 and discharged through the filter part 45.
  • the centrifugal liquid accommodating part 53 which accommodates () is formed.
  • the accumulation container 51 is located adjacent to the outlet 25 of the pretreatment container 11a and is provided to be inclined with respect to the axis of the outlet 25.
  • the integrated container 51 has the same shape and structure as the integrated container according to the first embodiment described above, and a description thereof will be omitted below.
  • FIG. 13 is a longitudinal sectional view showing the centrifuge kit 10c according to the third embodiment of the present invention mounted on the rotor 101b of the centrifuge.
  • the rotor 101b of the centrifuge has a disk shape, and has a central hole 103 formed at the center thereof, which can be combined with a rotational axis (not shown) of the motor, and pretreatment in the outer radial direction about the central hole 103.
  • the container seating portion 105 and the container seating portion 111 in which the container 11c, the integrated container 51 are sequentially seated and disposed are formed.
  • the plurality of container seating portions 105 and the plurality of container seating portions 111 are provided at equal intervals along the circumference of the central hole 103.
  • the pretreatment container 11c is seated on the container seating portion 105 such that the injection portion 23 of the pretreatment container 11c is positioned adjacent to the central hole 103.
  • the container support 113 is provided in the container mounting part 111 of the rotor 101b so that the integration container 51 may be inclined with respect to the axis line of the outflow part 25 of the pretreatment container 11c.
  • the pretreatment container 11c and the integrated container 51 are seated on the container seating portion 105 and the container seating portion 111 of the rotor 101b, respectively, and then biological or chemical substances such as nucleic acids for centrifugation are removed.
  • the rotor 101b of the centrifuge is rotated in one direction, and the mixed solution contained in the pretreatment container 11c is carried out.
  • (3) is centrifuged while being filtered through the filter unit 45 by centrifugal force, and then the centrifuged centrifugal liquid 5 passes through the outlet 25 of the pretreatment container 11c. Is discharged towards.
  • the rotation of the rotor 101b of the centrifuge is stopped.
  • the desired centrifugal liquid 5 contained in the centrifugal liquid container 53 of the integrated container 51 can be obtained.
  • the sample and the buffer having the biological or chemical substance for centrifugation are injected through the injection unit 15 formed in the upper plate of the pretreatment container 11c, and the integrated container 51 is disposed at the rear end of the pretreatment container 11c.
  • the sample and the buffer is easy to inject, improve the workability, facilitate the automation as well as to reduce the overall length of the centrifugation kit (10c) compared to the above-described embodiments.
  • FIG. 14 illustrates a centrifugal separation kit 10d having a circular cross-sectional shape of a pretreatment container 11d as a centrifugal separation kit according to a fourth embodiment of the present invention.
  • centrifugal separation kit 10d differs only in shape from the pretreatment container 11c of the third embodiment, other configurations are the same, the description thereof will be omitted below.
  • the pretreatment container may have an elliptical cross-sectional shape in addition to the rectangular and circular cross-sectional shapes described above.
  • the mixed solution 3 contained in the chamber 21 is injected into the injection unit 23 unlike the first embodiment described above. It further comprises a stopper 29 for preventing the leakage.
  • the barrier rib 29 is recessed in a cross-sectional shape of a 'V' shape on the side wall portion 13c, for example, on the side wall portion 13c positioned opposite to the side wall portion 13a on which the outlet portion 25 is formed. It is.
  • the jaw 29 has the inlet 23 and the outlet 25 so that the mixed solution 3 in the chamber 21 flows smoothly toward the outlet 25 by the centrifugal force generated during centrifugation. Located in between.
  • the jaw 29 is described as being recessed in one side wall portion 13c in the present embodiment, the present invention is not limited thereto, and the jaw 29 protrudes in a cross-sectional shape of a 'V' shape inside the pretreatment container 11e. Or it may be formed to protrude in a band shape having a predetermined length and width.
  • the pretreatment container (11e) of the centrifugal separation kit (10e) is mutually with the fixing jaw 115 located below the rotor 101a (see Fig. 7) of the centrifuge during centrifugation. It further includes a protruding rib 27 protruding from the pretreatment container 11e so as to swing the pretreatment container 11e by the action.
  • the protruding ribs 27 in the present embodiment protrude from one side wall portion 13a of the pretreatment container 11e positioned in the direction opposite to the injection portion 23, but the present invention is not limited thereto. It may be formed to protrude from the lower plate portion 17.
  • the pretreatment container 11e is mounted on the rotor 101a of the centrifuge and the pretreatment container 11e rotates during centrifugation, so that the protruding ribs 27 rotate within the rotation radius of the pretreatment container 11e. It passes over a plurality of fixing jaw 115 of the centrifuge located protruded at intervals toward the pretreatment container (11e) at the lower side of the), the pretreatment container (11e) is rocked, the chamber 21 of the pretreatment container (11e) The mixed solution 3 accommodated in is stirred smoothly.
  • the pretreatment container 11e of the centrifugal separation kit 10e further includes a protruding rib 27 and a jaw 29 so that the centrifuge kit As the pretreatment container 11e rotates during separation, the protruding rib 27 passes over the fixing jaw 27 and the pretreatment container 11e swings, so that the mixed solution 3 contained in the chamber 21 of the pretreatment container 11e. This is stirred smoothly.
  • the mixed solution 3 in the chamber 21 does not flow out into the inlet 23 by the jaw 29, but smoothly flows out of the outlet 25.

Landscapes

  • Centrifugal Separators (AREA)

Abstract

La présente invention porte sur un kit de centrifugation, lequel kit comprend un récipient de prétraitement et une colonne de centrifugation, le récipient de prétraitement comprenant : une chambre pour recevoir une solution mélangée qui contient un spécimen ayant un matériau biologique ou chimique pour la centrifugation et un agent tampon ; une partie d'injection à travers laquelle le spécimen et l'agent tampon sont injectés dans la chambre ; et une partie d'évacuation à travers laquelle la solution mélangée dans la chambre est évacuée par une force centrifuge générée pendant la centrifugation, et la colonne de centrifugation comprenant : une partie d'introduction à travers laquelle la solution mélangée évacuée à travers la partie d'évacuation est introduite ; une partie de réception de solution mélangée pour recevoir la solution mélangée introduite à partir de la partie d'introduction ; une partie de drain à travers laquelle une solution de séparation centrifuge, séparée de façon centrifuge à partir de la solution mélangée, est drainée ; et une partie filtre disposée entre la partie d'introduction et la partie de drain pour filtrer la solution mélangée devant être séparée de façon centrifuge. Par conséquent, il est possible de faciliter l'injection d'un spécimen et d'un agent tampon pour la centrifugation, d'améliorer l'aptitude au travail et d'obtenir facilement une automatisation.
PCT/KR2014/005156 2014-02-25 2014-06-12 Kit de centrifugation WO2015129965A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20140021828A KR101481539B1 (ko) 2013-05-15 2014-02-25 원심분리키트
KR10-2014-0021828 2014-02-25

Publications (1)

Publication Number Publication Date
WO2015129965A1 true WO2015129965A1 (fr) 2015-09-03

Family

ID=54010733

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2014/005156 WO2015129965A1 (fr) 2014-02-25 2014-06-12 Kit de centrifugation

Country Status (2)

Country Link
KR (1) KR101481539B1 (fr)
WO (1) WO2015129965A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108367302B (zh) * 2015-12-09 2020-08-25 麦迪康公司 离心分离机及离心分离方法
KR20180041384A (ko) * 2016-10-14 2018-04-24 (주)어핀텍 객담에서 결핵균 분리를 위한 객담 전처리 용기 및 이를 이용한 원심분리방법

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4787971A (en) * 1987-01-23 1988-11-29 Alan Donald Miniaturized column chromatography separation apparatus and method of assaying biomolecules employing the same
JPH0628749B2 (ja) * 1989-10-06 1994-04-20 ベックマン インスツルメンツ インコーポレーテッド 遠心機ロータ、その製造方法および密度勾配遠心分離法
US6103195A (en) * 1997-08-08 2000-08-15 Shukla; Ashok K. Micro-volume spin columns for sample preparation
KR101197974B1 (ko) * 2011-11-01 2012-11-05 박현정 신속한 원심분리가 가능한 원심분리용 용기

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4787971A (en) * 1987-01-23 1988-11-29 Alan Donald Miniaturized column chromatography separation apparatus and method of assaying biomolecules employing the same
JPH0628749B2 (ja) * 1989-10-06 1994-04-20 ベックマン インスツルメンツ インコーポレーテッド 遠心機ロータ、その製造方法および密度勾配遠心分離法
US6103195A (en) * 1997-08-08 2000-08-15 Shukla; Ashok K. Micro-volume spin columns for sample preparation
KR101197974B1 (ko) * 2011-11-01 2012-11-05 박현정 신속한 원심분리가 가능한 원심분리용 용기

Also Published As

Publication number Publication date
KR20140135093A (ko) 2014-11-25
KR101481539B1 (ko) 2015-01-14

Similar Documents

Publication Publication Date Title
US6238330B1 (en) Microcentrifuge
KR101361950B1 (ko) 유체 처리방법 및 유체 처리장치
US5571070A (en) Rotor sleeve for a centrifugal separator
WO2019132406A1 (fr) Méthode d'extraction d'acide nucléique à l'aide d'une cartouche
US5610074A (en) Centrifugal method and apparatus for isolating a substance from a mixture of substances in a sample liquid
JPH04227080A (ja) 特に全血から血漿を分離するのに適している不均一液体試料の２つの相を遠心分離によって分離する装置
WO1997012679A1 (fr) Systeme et procede de separation du sang par rotation axiale
WO2016105069A1 (fr) Appareil d'extraction d'acide nucléique et son procédé d'exploitation
WO2015129965A1 (fr) Kit de centrifugation
JPH07505956A (ja) 制流子付き分離装置
JP5583957B2 (ja) Ｍｗｐ蓋分離
WO2009151198A1 (fr) Analyseur chimique par lots
KR20040044981A (ko) 핵산 추출장치
KR101495631B1 (ko) 원심분리 키트 및 이를 이용한 원심분리 방법
WO2020036435A1 (fr) Ensemble tube destiné à goutter sur une bande de diagnostic et à extraire un fragment génétique
WO2013147515A1 (fr) Kit de séparation centrifuge et procédé de séparation centrifuge l'utilisant
WO2013176400A1 (fr) Récipient de séparation permettant de séparer en continu une particule/couche et procédé permettant de séparer une particule/couche par son utilisation
WO2021066506A1 (fr) Chambre pour centrifugeuse et centrifugeuse la comprenant
WO2011122841A2 (fr) Colonne permettant l'extraction de substances biochimiques d'échantillons biologiques
JPH07501150A (ja) 接合型微量溶液処理システムおよび処理方法
WO2011055873A1 (fr) Appareil pour extraire un materiau biochimique d'un echantillon biologique
WO2021071172A1 (fr) Appareil et méthode de séparation sans biais et continue de cellules tumorales circulantes
WO2017099487A1 (fr) Centrifugeuse et procédé de centrifugation
WO2020166980A2 (fr) Dispositif d'extraction, procédé d'extraction et puce fluidique pour extraire une matière cible
KR101102593B1 (ko) 3상 연속 원심분리기용 로터

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 14884226

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 14884226

Country of ref document: EP

Kind code of ref document: A1