WO2016101430A1 - 定量移液管 - Google Patents

定量移液管 Download PDF

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Publication number
WO2016101430A1
WO2016101430A1 PCT/CN2015/075083 CN2015075083W WO2016101430A1 WO 2016101430 A1 WO2016101430 A1 WO 2016101430A1 CN 2015075083 W CN2015075083 W CN 2015075083W WO 2016101430 A1 WO2016101430 A1 WO 2016101430A1
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WIPO (PCT)
Prior art keywords
quantitative
capillary
reservoir
tube
pipette according
Prior art date
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PCT/CN2015/075083
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English (en)
French (fr)
Inventor
马荣华
Original Assignee
东台市科华医疗器械有限公司
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Application filed by 东台市科华医疗器械有限公司 filed Critical 东台市科华医疗器械有限公司
Priority to US14/763,131 priority Critical patent/US20160339421A1/en
Publication of WO2016101430A1 publication Critical patent/WO2016101430A1/zh

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Definitions

  • This invention relates to laboratory equipment, and more particularly to an improved quantitative pipette.
  • Quantitative pipetting is a work that is always done in all physical, chemical and biological laboratories.
  • Large-capacity pipetting uses glass or plastic pipettes at home and abroad. Small or small amounts of pipetting are available at home and abroad.
  • some domestic and foreign users have used self-priming micro-scale blood collection tubes produced by our Dongtai Kehua Medical Devices Co., Ltd. for micropipetting, but for the need for pipetting
  • the amount is large, for example, at 50-1000 ⁇ l, the automatic aspiration can not be completed by the capillary action of the self-priming quantitative micro blood collection tube.
  • pipettes that can easily and accurately complete the removal of large amounts of liquid specimens have not been seen at home and abroad.
  • the technical problem to be solved by the present invention is to provide a quantitative pipette which can easily and accurately complete the removal of small amounts of liquid, has the advantages of easy operation, low cost and no need. Any other auxiliary equipment and work efficiency characteristics, especially in biological experiments can avoid the possibility of cross-contamination.
  • the solution to the technical problem of the present invention is: the quantitative pipette provided by the present invention, which comprises an air bag, an air guiding tube, a reservoir and a quantitative capillary, the air bag, the air guiding tube and the storage
  • the sacs are made of a transparent polymer material and are sequentially connected and communicated with the inner holes.
  • the quantitative capillary is made of a transparent polymer material or a glass material, and the upper end of the quantitative capillary is inserted into the interior of the reservoir and close to the The airway is described, the lower end of the metering capillary extending out of the reservoir, and the volume of the metering capillary is selected according to the capacity of the desired pipetting liquid.
  • the product of the cross-sectional area and length of the quantitative capillary lumen is the volume of the quantitative capillary.
  • the quantitative capillary is a hollow circular tube, and the cross-sectional area of the quantitative capillary lumen is determined by the inner diameter of the quantitative capillary.
  • At least one end of the metering capillary is tapered.
  • the quantitative capillary has a tapered end and a cylindrical shape in the middle.
  • the volume of the quantitative capillary is between 30 and 1000 microliters.
  • the upper portion of the reservoir is ellipsoidal and the lower portion is a hollow circular tube.
  • both ends of the upper portion of the reservoir are tapered, the middle portion of the upper portion of the reservoir is cylindrical, and the lower portion of the reservoir is a hollow circular tube.
  • the outer wall of the lower portion of the metering capillary partially conforms to the inner wall of the lower portion of the reservoir and is secured together by an adhesive.
  • the air bag is flat or ellipsoidal for easy pinching with a finger during operation.
  • the airbag When using the above quantitative pipette provided by the present invention, the airbag is lightly squeezed, the lower end of the quantitative capillary is immersed in the sucked liquid, and the airbag is gently released, and the liquid is sucked into the quantitative capillary, and when it is full, excess liquid It will overflow from the top of the quantitative capillary and flow into the reservoir.
  • the quantitative capillary When the aspiration stops, the quantitative capillary is filled with the absorbed liquid. Its capacity is a preset capacity, and some excess liquid remains in the reservoir.
  • FIG. 1 is a schematic structural view of a quantitative pipette according to an embodiment of the present invention (the quantitative capillary is a hollow circular tube);
  • FIG. 2 is a schematic view showing the structure of a quantitative pipette according to another embodiment of the present invention (the quantitative capillary has a tapered end).
  • the present invention provides a quantitative pipette comprising a balloon 1, an airway tube 2, a reservoir 3 and a quantitative capillary 4, wherein the balloon 1, the airway tube 2 and the reservoir 3 are transparent polymers.
  • the material is made and sequentially connected and the inner holes are communicated.
  • the quantitative capillary 4 is made of a transparent polymer material or a glass material, and the upper end of the quantitative capillary 4 is inserted into the interior of the reservoir 3 and adjacent to the air tube 2, the quantitative capillary The lower end of 4 projects out of said reservoir 3, and the volume of the metering capillary 4 is selected in accordance with the capacity of the desired pipetting liquid.
  • the upper end of the quantitative capillary 4 is inserted into the inside of the reservoir 3 and close to the air tube 2, and the lower end thereof protrudes from the reservoir 3, that is, the upper end of the quantitative capillary 4 is located in the reservoir 3.
  • the inside is close to the above-described air guiding tube 2 and is kept at a certain distance so that excess liquid in the inhalation quantitative capillary 4 overflows from the tip end of the quantitative capillary 4 and flows into the reservoir 3.
  • the product of the cross-sectional area and length of the inner cavity of the quantitative capillary 4 is the volume of the above-described quantitative capillary 4.
  • the above-mentioned quantitative capillary 4 is a hollow circular tube (i.e., the above-described quantitative capillary 4 is cylindrical in shape), and the cross-sectional area of the inner cavity of the quantitative capillary 4 is determined by the inner diameter of the quantitative capillary 4.
  • the quantitative capillary tube 4 has a tapered shape at both ends and a cylindrical shape in the middle.
  • the overall shape of the quantitative capillary tube 4, the shape of one or both ends thereof, and the shape of the middle are not limited to a cylindrical shape or a tapered shape, and may be other shapes existing in the prior art, such as two of them.
  • the end may be wedge-shaped or the like as long as it does not affect the suction and release of the liquid by the capillary tube 4, the upper end thereof is not in contact with the reservoir 3 and the air tube 2, and the outer wall of the lower portion and the lower portion of the reservoir 3 are not affected.
  • the inner wall may be partially bonded and fixed by adhesion.
  • the volume of the above-mentioned quantitative capillary 4 is 30 to 1000 ⁇ l. Therefore, the quantitative pipette provided by the present invention can be made into pipettes of various capacity specifications, such as 30 microliters, 40 microliters, 50 microliters, and 1000 microliters, depending on the needs of the user.
  • the upper portion of the reservoir 3 is ellipsoidal and the lower portion is a hollow circular tube.
  • Both ends of the upper portion of the reservoir 3 are tapered, the middle portion of the upper portion of the reservoir 3 is cylindrical, and the lower portion of the reservoir 3 is a hollow circular tube.
  • the upper portion of the reservoir 3 is ellipsoidal, or the upper ends of the upper portion of the reservoir 3 are tapered in the middle, but the shape of the upper portion of the reservoir 3 is not It is limited to the above two shapes, but it is preferably the above two shapes, which may be any shape existing in the prior art as long as the upper end of the quantitative capillary 4 can be placed in the center of the inside of the reservoir 3, without being in contact with The inner wall of the reservoir 3 does not hinder the flow of excess liquid into the reservoir 3 smoothly when the liquid is sucked.
  • the upper ends of the upper portion of the reservoir 3 are semispherical and have a cylindrical shape.
  • the outer wall of the lower portion of the above-described quantitative capillary 4 is partially adhered to the inner wall of the lower portion of the reservoir 3 and fixed together by an adhesive to prevent excess liquid inside the reservoir 3 from flowing out of the environment.
  • the airbag 1 described above is flat or ellipsoidal in order to be pinched and fixed with a finger during operation.
  • the airbag 1 is flat or ellipsoidal, but the shape of the airbag 1 is not limited to a flat shape or an ellipsoid shape, but is preferably a flat shape or an ellipsoid shape, which may be existing in the prior art. In other shapes, the airbag 1 may be pinched and fixed by a finger as long as it is convenient for the user to operate. For example, the airbag 1 has a spherical shape or a cone shape.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

一种定量移液管，包括气囊（1）、导气管（2）、储液囊（3）和定量毛细管（4），气囊（1）、导气管（2）和储液囊（3）均为透明高分子材料制成且依次连接并内孔相通，定量毛细管（4）为透明高分子材料或玻璃材料制成，定量毛细管（4）的上端插入储液囊（3）的内部且靠近导气管（2），其下端伸出储液囊（3），定量毛细管（4）的容积根据所需吸移液体的容量而选定。

Description

定量移液管 技术领域
本发明涉及实验器材，尤其涉及一种改进的定量移液管。
背景技术
定量移液为所有物理、化学及生物实验室内时时刻刻都在做的工作，大容量的移液国内外都使用玻璃或塑料的移液管，小容量或微量的移液国内外都在使用微量移液器配上一次性的吸嘴使用，近年来部分国内外用户对微量移液也有在使用我们东台市科华医疗器械有限公司生产的自吸定量微量采血管，但是对于需要移液量较大时，如在50-1000μl时则无法通过自吸定量微量采血管的毛细作用完成自动吸样。目前可以轻松、准确完成较大微量液体标本移取的移液管国内外尚未见到。
发明内容
针对现有技术所存在的上述不足，本发明所要解决的技术问题是要提供一种定量移液管，它可以轻松、准确的完成小微量液体的移取，具有易于操作、成本低廉、不需其它任何辅助设备和提高工作效率的特点，特别是在生物实验中可以避免产生交叉污染的可能。
为了解决上述技术问题，本发明解决技术问题的方案是：本发明提供的定量移液管，其包括气囊、导气管、储液囊和定量毛细管，所述气囊、所述导气管和所述储液囊均为透明高分子材料制成且依次连接并内孔相通，所述定量毛细管为透明高分子材料或玻璃材料制成，所述定量毛细管的上端插入所述储液囊的内部且靠近所述导气管，所述定量毛细管的下端伸出所述储液囊，所述定量毛细管的容积根据所需吸移液体的容量而选定。
进一步地，所述定量毛细管内腔的横截面面积和长度的积为所述定量毛细管的容积。
优选地，所述定量毛细管为中空圆管，所述定量毛细管内腔的横截面面积由所述定量毛细管的内径确定。
可选地，所述定量毛细管的至少一端为锥形。
优选地，所述定量毛细管的两端为锥形，中间为圆柱形。
可选地，所述定量毛细管的容积为30-1000微升。
优选地，所述储液囊的上部分为椭球形，其下部分为中空圆管。
优选地，所述储液囊的上部分的两端为锥形，所述储液囊的上部分的中间为圆柱形，所述储液囊的下部分为中空圆管。
进一步地，所述定量毛细管的下部分的外壁与所述储液囊的下部分的内壁部分地贴合且通过粘合剂固定在一起。
可选地，所述气囊为扁平状或椭球状，以便于操作时用手指挤捏固定。
使用本发明提供的上述定量移液管时，轻捏气囊，将定量毛细管的下端浸入被吸的液体中，轻轻松开气囊，液体就会被吸入定量毛细管，当其吸满时，多余的液体就会从定量毛细管顶端溢出并流入到储液囊中，当吸液停止后定量毛细管中充满被吸液体，其容量为预先设定好的容量，储液囊中留有部分多余液体，此时再轻捏气囊就可以将定量毛细管中的液体全部滴出，从而轻松、准确地完成微量液体的定量移取。
附图说明
图1是本发明一实施例提供的定量移液管的结构示意图(其定量毛细管为中空圆管)；
图2是本发明另一实施例提供的定量移液管的结构示意图(其定量毛细管的一端为锥形)。
具体实施方式
参照图1，本发明提供了一种定量移液管，其包括气囊1、导气管2、储液囊3和定量毛细管4，上述气囊1、导气管2和储液囊3均为透明高分子材料制成且依次连接并内孔相通，上述定量毛细管4为透明高分子材料或玻璃材料制成，该定量毛细管4的上端插入上述储液囊3的内部且靠近上述导气管2，该定量毛细管4的下端伸出上述储液囊3，该定量毛细管4的容积根据所需吸移液体的容量而选定。
需要说明的是，上述定量毛细管4的上端插入上述储液囊3的内部且靠近上述导气管2，其下端伸出上述储液囊3，即上述定量毛细管4的上端位于上述储液囊3的内部接近上述导气管2并保留一定的距离，以使吸入定量毛细管4内多余的液体从该定量毛细管4的顶端溢出并流入到上述储液囊3中。
上述定量毛细管4内腔的横截面面积和长度的积为上述定量毛细管4的容积。
参照图1，上述定量毛细管4为中空圆管(即，上述定量毛细管4的形状为圆柱形)，该定量毛细管4内腔的横截面面积由该定量毛细管4的内径确定。
参照图2，上述定量毛细管4的至少一端为锥形。可选地，上述定量毛细管4的一端为锥形。优选地，上述定量毛细管4的下端为锥形(即图2所示)。可选地，上述定量毛细管4的两端为锥形，中间为圆柱形。
需要注意的是，上述定量毛细管4的整体形状、其一端或两端的形状以及中间的形状，均并不局限于圆柱形或锥形，可以是现有技术中已有的其他形状，比如其两端可为楔形等，只要不影响该定量毛细管4吸取和释放液体、其上端不与上述储液囊3和导气管2接触以及不影响其下部分的外壁与上述储液囊3的下部分的内壁部分地贴合、粘合固定即可。
上述定量毛细管4的容积为30-1000微升。因此，本发明提供的定量移液管可以制成各种容量规格的移液管，比如30微升、40微升、50微升……以及1000微升，可根据用户的需求而定。
上述储液囊3的上部分为椭球形，其下部分为中空圆管。
上述储液囊3的上部分的两端为锥形，该储液囊3的上部分的中间为圆柱形，该储液囊3的下部分为中空圆管。
需要注意的是，上述储液囊3的上部分为椭球形，或者上述储液囊3的上部分的两端为锥形中间为圆柱形，但上述储液囊3的上部分的形状并不局限于上述这两种形状，只是优选为上述这两种形状，其可以是现有技术中已有的任何形状，只要能够使得定量毛细管4的上端处于储液囊3的内部中央，不接触到储液囊3的内壁，不阻碍吸液时多余的液体顺利地流入该储液囊3中即可，比如上述储液囊3的上部分的两端为半球形中间为圆柱形等。
上述定量毛细管4的下部分的外壁与上述储液囊3的下部分的内壁部分地贴合且通过粘合剂固定在一起，以防止储液囊3内部的多余液体流出污染环境。
上述气囊1为扁平状或椭球状，以便于操作时用手指挤捏固定。
需要注意的是，上述气囊1为扁平状或椭球状，但上述气囊1的形状并不局限于扁平状或椭球状，只是优选为扁平状或椭球状，其可以是现有技术中已有的其他形状，只要能够方便用户操作时用手指挤捏固定上述气囊1即可，比如上述气囊1为圆球状(形)或锥球状(形)。

Claims (10)

  1. 一种定量移液管，其包括气囊(1)、导气管(2)、储液囊(3)和定量毛细管(4)，所述气囊(1)、所述导气管(2)和所述储液囊(3)均为透明高分子材料制成且依次连接并内孔相通，所述定量毛细管(4)为透明高分子材料或玻璃材料制成，所述定量毛细管(4)的上端插入所述储液囊(3)的内部且靠近所述导气管(2)，所述定量毛细管(4)的下端伸出所述储液囊(3)，所述定量毛细管(4)的容积根据所需吸移液体的容量而选定。
  2. 根据权利要求1所述的定量移液管，其中，所述定量毛细管(4)内腔的横截面面积和长度的积为所述定量毛细管(4)的容积。
  3. 根据权利要求2所述的定量移液管，其中，所述定量毛细管(4)为中空圆管，所述定量毛细管(4)内腔的横截面面积由所述定量毛细管(4)的内径确定。
  4. 根据权利要求3所述的定量移液管，其中，所述定量毛细管(4)的至少一端为锥形。
  5. 根据权利要求4所述的定量移液管，其中，所述定量毛细管(4)的两端为锥形，中间为圆柱形。
  6. 根据权利要求1至5中任一项所述的定量移液管，其中，所述定量毛细管(4)的容积为30-1000微升。
  7. 根据权利要求1所述的定量移液管，其中，所述储液囊(3)的上部分为椭球形，其下部分为中空圆管。
  8. 根据权利要1所述的定量移液管，其中，所述储液囊(3)的上部分的两端为锥形，所述储液囊(3)的上部分的中间为圆柱形，所述储液囊(3)的下部分为中空圆管。
  9. 根据权利要求7或8所述的定量移液管，其中，所述定量毛细管(4)的下部分的外壁与所述储液囊(3)的下部分的内壁部分地贴合且通过粘合剂固定在一起。
  10. 根据权利要求1所述的定量移液管，其中，所述气囊(1)为扁平状或椭球状，以便于操作时用手指挤捏固定。
PCT/CN2015/075083 2014-12-23 2015-03-26 定量移液管 WO2016101430A1 (zh)

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US14/763,131 US20160339421A1 (en) 2014-12-23 2015-03-26 Quantitative pipette

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CN201410820521.1A CN105498877A (zh) 2014-12-23 2014-12-23 定量移液管
CN201410820521.1 2014-12-23

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