WO2016099073A1 - Nouvelles communautés microbiennes de granules pour la production d'hydrogène et leur procédé de préparation - Google Patents

Nouvelles communautés microbiennes de granules pour la production d'hydrogène et leur procédé de préparation Download PDF

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Publication number
WO2016099073A1
WO2016099073A1 PCT/KR2015/013489 KR2015013489W WO2016099073A1 WO 2016099073 A1 WO2016099073 A1 WO 2016099073A1 KR 2015013489 W KR2015013489 W KR 2015013489W WO 2016099073 A1 WO2016099073 A1 WO 2016099073A1
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Prior art keywords
hydrogen
hydrogen production
injecting
granular microbial
producing
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PCT/KR2015/013489
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English (en)
Korean (ko)
Inventor
김상현
쿠말고팔라크리쉬난
박정훈
페리야사미시바구루나탄
박종훈
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대구대학교 산학협력단
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Publication of WO2016099073A1 publication Critical patent/WO2016099073A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P3/00—Preparation of elements or inorganic compounds except carbon dioxide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Definitions

  • the present invention relates to a novel granular microbial colony for hydrogen production and a method for manufacturing the same, and more particularly, a granular microbial colony that maintains stable and high production rate without loss of microorganisms for a short residence time and a short time after the colony collapses. It provides a method for granulating hydrogen-producing microorganisms having a feature that granulation between microorganisms can proceed.
  • biomass as an alternative energy source is important because it can obtain chemicals such as carbon, hydrogen and oxygen from waste resources as well as plants such as surrounding grass and trees.
  • the photo-fermentation method has a high theoretical hydrogen conversion rate, but it is difficult to use complex organic materials, whereas the anaerobic fermentation method does not require light energy and controls pH, hydraulic retention time, etc. Inhibits the production of the final by-product methane and produces hydrogen.
  • the anaerobic fermentation method has a lower hydrogen conversion rate than photofermentation, but can produce hydrogen more stably by using organic materials directly (FR Hawkes, et al., Sustainable fermentative hydrogen production: challenges for process optimization, Int. J. of Hydrogen Energy, Vol . 27, p. 1339-1347, 2002).
  • the UASB (upflow anaerobic sludge blanket) reactor has been used to treat a wide range of wastewater from low concentration wastewater, such as beer wastewater, to high concentration wastewater, such as alcoholic wastewater, due to high sludge retention due to granulation formation (Harada, H., et al., Interaction between sulfate-reducing bacteria and methane-producing bacteria in UASB reactors fed with low strength wastes containing different levels of sulfate, Wat.
  • Upflow Bacterial cells in the stream aggregate into dense particles called granular sludge, resulting in less microbial outflow in the reactor, densifying cells and improving the transfer of metabolites to the wastewater.
  • Mainly used (Guiot, SR, Y. Arcand and C. Chavarie Advantages of fluidization on granule size and activity development in Upflow Sludge Bed activity development in Upflow Sludge Bed reactors.Wat.Sci.Tech., 26 (3-4), 897-906, 1992).
  • Continuous anaerobic hydrogen fermentation process and a method for producing hydrogen using the same for the treatment of organic waste in the prior art of the present invention has been known in the Republic of Korea Patent No. 10-0553231, but it is organic waste by continuously circulating the organic waste at a double temperature
  • the present invention relates to a process for producing a new hydrogen fermentation process and a method for producing hydrogen using the same for generating hydrogen in the treatment process.
  • a biological hydrogen production method for increasing hydrogen production by using a trickling bed reactor has been known in Korea Patent No. 10-0813151, but this is to find the optimum operating conditions of the trickling bed reactor to increase the concentration of hydrogen producing microorganisms.
  • the present invention relates to a method for increasing hydrogen production.
  • an apparatus and method for producing bio-hydrogen gas from organic waste using methane producing microbial activity inhibitor and gas purging under low pH conditions are known from Korean Patent Registration No. 10-0990167, which merely inhibits the activity of methane producing microorganisms.
  • injection of an active inhibitor minimizes methane conversion of hydrogen by methane-producing microorganisms and lowers the partial pressure of hydrogen by intermittent gas purging to increase the activity of hydrogen-producing microorganisms.
  • a high concentration hydrogen production system and a method for producing hydrogen using waste glycerol as a by-product of biodiesel production are known from Korean Patent No. 10-1037512, but it is a superlative waste waste glycerol that is generated as a by-product during biodiesel production. It relates to a hydrogen production system and a hydrogen production method for producing a synthesis gas containing a high concentration of hydrogen by gasification in the coefficient.
  • Granular microbial community for hydrogen production of the present invention comprises the steps of dissolving by injecting alginic acid and distilled water for the attachment of microorganisms; Dissolving again by injecting silicon dioxide into the solution obtained in the above step; Injecting the activated carbon and the sludge collected in the anaerobic reactor in the sewage treatment plant into the dissolved solution obtained in the above step to dissolve again to generate activated carbon-attached microorganisms; Injecting chitosan into the dissolved solution of the activated carbon-attached microorganisms obtained in the above step to dissolve and inducing calcium microorganism by injecting calcium chloride; After injecting the granular microbial colony obtained in the above step into the culture tank was achieved by operating through a stirrer at a rate of 150 ⁇ 200 RPM through the step of culturing the granular microbial colony.
  • the present invention has the effect of stable hydrogen production without the loss of microorganisms even in a short hydraulic residence time, which is advantageous for hydrogen production in the production of biological hydrogen, and the granulation between the microorganisms within a short time even after the collapse of the granular microorganisms generated There is an excellent effect of providing a method for producing a hydrogen-producing granular microbial community.
  • 1 shows a process for producing a microbial community for hydrogen production according to the present invention
  • 1 is sodium alginate
  • 2 is silicon dioxide
  • 3 is activated carbon
  • 4 is chitosan
  • 5 is calcium chloride
  • 6 is a granular microorganism culture tank
  • 7 Is a diagram showing a stirrer.
  • FIG. 2 is a graph showing the hydrogen fraction according to the hydraulic retention time change according to the present invention.
  • Figure 3 is a graph showing the hydrogen production yield after the collapse of the particulate microbial community according to the present invention.
  • the method for producing a microbial population for hydrogen production is composed of sodium alginate, silicon dioxide, activated carbon, chitosan, calcium chloride, granular microorganism culture tank, agitator and the sodium alginate
  • silicon dioxide serves to improve the physical strength of the hydrogen-producing granular microbial community outside, it is most preferable to mix the w / w ratio of sodium alginate 2%, the w / w ratio of silicon dioxide 1%.
  • the activated carbon serves to provide voids in the hydrogen-producing particulate microbial population in the colony, preferably in the form of a powder, and most preferably, the w / w ratio of the activated carbon is mixed at 2%.
  • the chitosan maintains the lattice shape at the outermost of the colony to maintain the shape of the microbial colony, to prevent the destruction and leakage of the colony, and the w / w ratio of the chitosan is most preferably mixed at 1%.
  • the mixed solution remains in a gel form, which is dropwise added to the calcium chloride in a bead form using a pipet or a syringe.
  • sodium alginate and calcium chloride react to form a converted and cured form of calcium alginate, and the w / w ratio of calcium chloride is most preferably mixed at 2%.
  • the granular microorganism culture tank follows the form of a complete mixed reactor (STR) and can be used in the form of a continuous complete mixed reactor (CSTR) as needed.
  • CSTR continuous complete mixed reactor
  • the outlet of the nutrients should be located in the nutrient system, and in the case of hydrogen producing microbial populations, anaerobic microorganisms are mainly used. It must be kept closed except in the form.
  • the microbial nutrients and the hydrogen-producing microbial community coexist in the granular microbial culture tank to provide the optimum conditions for the hydrogen-producing microbial community to cultivate.
  • the ratio is most preferably adjusted to 59: 35: 6.
  • the pH of the granular microorganism culture tank is preferably maintained at 5.5, and the pH sensor and the alkali injection tank therefor may be further installed under conditions capable of maintaining anaerobic conditions.
  • the pH sensor and alkali can use the finished product and it is most preferable to use 1M NaOH for alkali.
  • the stirrer is most preferably positioned at the lower end of the granular microorganism culture tank to minimize the four sections and maintain the rotational speed at 150 to 200 RPM to smoothly mix.
  • Alginic acid, distilled water and silicon dioxide for the attachment of microorganisms according to the present invention is 2: 97: 1 by weight, but the injection sequence is stirred at 50 until all the alginic acid is dissolved in distilled water, and then silicon dioxide is injected and dissolved.
  • 2% (w / w) of activated charcoal and 5% (w / w) of dried sludge at 105 were collected from the anaerobic reactor in the sewage treatment plant. Chitosan is injected into the solution in which the activated carbon-attached microorganism is produced in 1% (w / w) to dissolve, and then calcium chloride is injected into 2% (w / w) to induce granular microbial encapsulation.
  • the stirrer was operated at a speed of 150 ⁇ 200 RPM to incubate the granular microbial colony up to a size of 5-6 mm.
  • the present invention has the effect that the microorganisms can be stably produced without loss of microorganisms even in a short hydraulic residence time, which is advantageous for the hydrogen production in the production of biological hydrogen, and granules between microorganisms within a short time even after the collapse of the generated granular microbial community. It is a very useful invention in the environmental energy industry because it has an excellent effect of providing a method for producing a hydrogen-producing granular microbial population that proceeds the oxidation.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé pour induire la croissance, et la culture, de communautés microbiennes de granules pour la production d'hydrogène, ayant pour effet de permettre la production stable d'hydrogène sans perdre de microbes même avec un court temps de rétention hydraulique, ce qui est avantageux pour la productivité d'hydrogène dans le cadre d'une production biologique d'hydrogène, et un excellent effet d'obtention d'un procédé de préparation de communautés microbiennes de granules pour la production d'hydrogène, le procédé procédant à la granulation parmi les microbes en un court laps de temps même après effondrement des communautés microbiennes de granules.
PCT/KR2015/013489 2014-12-16 2015-12-10 Nouvelles communautés microbiennes de granules pour la production d'hydrogène et leur procédé de préparation WO2016099073A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2014-0181832 2014-12-16
KR1020140181832A KR20160073508A (ko) 2014-12-16 2014-12-16 신규한 수소생산용 입상 미생물 군집체 및 그 제조방법

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20060110394A (ko) * 2005-04-19 2006-10-25 한국에너지기술연구원 혐기 미생물 복합체를 이용한 수소의 제조방법
KR100813151B1 (ko) * 2006-12-29 2008-03-17 한국과학기술연구원 트리클링 베드 반응기를 사용하여 수소 생산량을 증대하기위한 생물학적 수소 생산방법
KR20080063960A (ko) * 2007-01-03 2008-07-08 연세대학교 산학협력단 수소 제조용 미생물 담지 복합막 반응기 및 장치

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20060110394A (ko) * 2005-04-19 2006-10-25 한국에너지기술연구원 혐기 미생물 복합체를 이용한 수소의 제조방법
KR100813151B1 (ko) * 2006-12-29 2008-03-17 한국과학기술연구원 트리클링 베드 반응기를 사용하여 수소 생산량을 증대하기위한 생물학적 수소 생산방법
KR20080063960A (ko) * 2007-01-03 2008-07-08 연세대학교 산학협력단 수소 제조용 미생물 담지 복합막 반응기 및 장치

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KUMAR, GOPALAKRISHNAN ET AL.: "Fabrication of Hybrid Immobilized Cells and Their Microbial Community Response to Various Processes Disturbances in a High-rate Hydrogen Producing Reactor Fed with Galactose", INTERNATIONAL CONFERENCE ON EMERGING TRENDS IN BIOTECHNOLOGY (ICETB 2014), November 2014 (2014-11-01), New Delhi *
LEE, JUNG - YEOL ET AL.: "Biological Hydrogen Production from Mixed Waste in a Polyuretbane Foam-sequencing Batch Reactor", KOREAN JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, vol. 42, no. 3, September 2014 (2014-09-01), pages 307 - 311 *
SLY AGURUNATHAN, PERIYASAMY ET AL.: "Development of a Novel Hybrid Immobilization Material (HY-IM) for Fermentative Biohydrogen Production from Beverage Wastewater", JOURNAL OF THE CHINESE CHEMICAL SOCIETY, vol. 61, no. 7, April 2014 (2014-04-01), pages 827 - 830 *

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