WO2016097857A1 - Compositions et leurs procédés d'utilisation - Google Patents

Compositions et leurs procédés d'utilisation Download PDF

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Publication number
WO2016097857A1
WO2016097857A1 PCT/IB2015/002477 IB2015002477W WO2016097857A1 WO 2016097857 A1 WO2016097857 A1 WO 2016097857A1 IB 2015002477 W IB2015002477 W IB 2015002477W WO 2016097857 A1 WO2016097857 A1 WO 2016097857A1
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composition
alkyl
microorganism
transport system
sulfonic acids
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PCT/IB2015/002477
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English (en)
Inventor
Paul Geoffrey SMYTH
Michael BRALKOWSKI
Graeme George PAINE
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Gfs Corporation Aus Pty Ltd
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Priority claimed from AU2014905067A external-priority patent/AU2014905067A0/en
Application filed by Gfs Corporation Aus Pty Ltd filed Critical Gfs Corporation Aus Pty Ltd
Priority to AU2015365575A priority Critical patent/AU2015365575A1/en
Publication of WO2016097857A1 publication Critical patent/WO2016097857A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
    • C11D1/02—Anionic compounds
    • C11D1/12—Sulfonic acids or sulfuric acid esters; Salts thereof
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
    • C11D1/02—Anionic compounds
    • C11D1/12—Sulfonic acids or sulfuric acid esters; Salts thereof
    • C11D1/22—Sulfonic acids or sulfuric acid esters; Salts thereof derived from aromatic compounds
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
    • C11D1/66—Non-ionic compounds
    • C11D1/83—Mixtures of non-ionic with anionic compounds
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/381—Microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/34—Processes using foam culture
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/02—Odour removal or prevention of malodour
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00—Use of specific compounds during water treatment
    • C02F2305/04—Surfactants, used as part of a formulation or alone
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
    • C11D2111/10—Objects to be cleaned
    • C11D2111/14—Hard surfaces
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
    • C11D2111/10—Objects to be cleaned
    • C11D2111/14—Hard surfaces
    • C11D2111/20—Industrial or commercial equipment, e.g. reactors, tubes or engines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus

Definitions

  • This invention relates to methods of treatment of a sewage transport system, including reducing odour, reducing hydrogen sulfide production, reducing and/or preventing corrosion, reducing and/or preventing biofilm formation and/or adhesion and disrupting biofilms.
  • compositions suitable for use in such methods comprising one or more anionic surfactants selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ester sulfonates or sulfonic acids and salts thereof, preferably with at least one microorganism of the genus Bacillus, are also described.
  • anionic surfactants selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ester sulfonates or sulfonic acids and salts thereof, preferably with at least one microorganism of the genus Bacillus, are also described
  • Septic sewage produces offensive odours such as the odour associated with hydrogen sulfide gas (rotten egg gas) and other malodourous gaseous substances.
  • odours can be toxic at specific concentrations, which presents a significant health risk, particularly for people working in and around the sewage transport system.
  • Hydrogen sulfide gas at 1000 ppm will cause instant death, at 500 ppm carries a significant mortality risk, at 100 ppm causes a loss of a sense of smell and at 10 ppm will react with mercury in teeth fillings and present other health risks.
  • dissolved sulfides such as HS " or H 2 S
  • sulfates such as S0 4 2" or H 2 SO 4
  • High levels of dissolved sulfides also inhibit sewage digestion by aerobic and anaerobic methane-forming bacteria and, therefore, may inhibit a significant component of the sewage decomposition process. Inhibition of the sewage decomposition process may result in high sludge volumes.
  • Hydrogen sulfide gas is a resulting product of the microbial activity of sulfur reducing bacteria in environments such as a sewage transport system (equations 1 and 2). Activity of other microorganisms may also contribute to the production of hydrogen sulfide gas through the reduction of the redox potential of the sewage, thereby creating optimal conditions for sulfate reduction (Neethling et al. 1989, Causes and Control of Concrete Pipe Corrosion ⁇ http://www.seas.ucla.edu/stenstro/r/r29>).
  • the sulfides may then be converted to sulfates, such as sulfuric acid, by another group of bacteria (equation 3).
  • Sulfuric acid causes significant corrosion within the sewage transport system, particularly of concrete and metal. Corrosion of the sewage transport system can be a source of significant cost through the continual maintenance and replacement of infrastructure. It is estimated that the cost of sewage infrastructure corrosion in the United States is US$13.75 billion/year.
  • compositions comprising one or more anionic surfactants selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ester sulfonates or sulfonic acids and salts thereof in an amount in the range of from 0.1% to 2% w/w and at least one microorganism of the genus Bacillus reduces biofilm formation or adhesion, disrupts biofilms, reduces odour and reduces or prevents corrosion in a sewage transport system.
  • anionic surfactants selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ester
  • a method of treatment of a sewage transport system comprising addition of a composition comprising one or more anionic surfactants selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ester sulfonates or sulfonic acids and salts thereof in an amount in the range of from 0.1% to 2% w/w and at least one microorganism of the genus Bacillus to the sewage transport system.
  • anionic surfactants selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ester sulfonates or sulfonic acids
  • composition comprising at least one microorganism of the genus Bacillus in an amount in the range of from 1 x 10 4 cfu/mL to 1 x 10 7 cfu/mL and one or more anionic surfactants selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ether sulfonates or sulfonic acids and salts thereof in an amount in the range of from 0.1 % to 2% w/w.
  • anionic surfactants selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ether s
  • alkyl refers to a straight chain or branched saturated hydrocarbon group having 1 to 20 carbon atoms. Where appropriate, the alkyl group may have a specified number of carbon atoms, for example, Cs-Cis alkyl which includes alkyl groups having 8, 9, 10, 11, 12, 13, 14, 15, 16, 17 or 18 carbon atoms in a linear or branched arrangement.
  • alkyl groups include, but are not limited to, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, t-butyl, octyl, decyl, dodecyl, tetradecyl, hexadecyl and octadecyl.
  • aryl is used herein to refer to any stable monocyclic or bicyclic carbon ring of up to 7 atoms in each ring, wherein at least one ring is aromatic. Examples of such aryl elements include, but are not limited to, phenyl, naphthyl, tetrahydronaphthyl, biphenyl and binaphthyl.
  • biosurfactant refers to a surfactant that is produced by bacteria, yeast or fungi.
  • the biosurfactant may be an antimicrobial peptide such as a cyclic lipopeptide biosurfactant including surfactin, lichenysin, iturin, fengycin, bacillomycin or mycosubtilin.
  • an antimicrobial peptide such as a cyclic lipopeptide biosurfactant including surfactin, lichenysin, iturin, fengycin, bacillomycin or mycosubtilin.
  • sewage transport system refers to the system of sewerage pipeline or sewage mains, man holes, hydraulic systems such as pumps, wet wells, pumping stations and lift stations that transport the sewage from its origin to the sewage treatment plant. This term refers to both gravity transport systems and pressure transport systems.
  • wet well refers to an underground pit for sewage storage, typically at a pumping station.
  • the present invention relates to the use of a composition
  • a composition comprising one or more anionic surfactants selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ester sulfonates or sulfonic acids and salts thereof in an amount in the range of from 0.1% to 2% w/w to reduce odour, reduce hydrogen sulfide production, reduce and/or prevent corrosion, reduce and/or prevent biofilm formation and/or adhesion and/or disrupt existing biofilms in a sewage transport system.
  • the composition useful in the invention further comprises at least one microorganism of the genus Bacillus.
  • a method of treatment of a sewage transport system comprising addition of a composition comprising one or more anionic surfactants selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ester sulfonates or sulfonic acids and salts thereof in an amount in the range of from 0.1% to 2% w/w to the sewage transport system.
  • anionic surfactants selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ester sulfonates or sulfonic acids and salts thereof in an amount in the range of from 0.1% to
  • a method of treatment of a sewage transport system comprising addition of a composition comprising one or more anionic surfactants selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ester sulfonates or sulfonic acids and salts thereof in an amount in the range of from 0.1% to 2% w/w and at least one microorganism of the genus Bacillus to the sewage transport system.
  • anionic surfactants selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ester sulfonates or sulfonic acids
  • the surfactant is an anionic surfactant selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ester sulfonates or sulfonic acids and salts or mixtures thereof.
  • Suitable alkyl sulfates include, but are not limited to, Cs-Cis alkyl sulfates such as sodium dodecyl sulfate, lithium dodecyl sulfate, ammonium dodecyl sulfate, sodium tetradecyl sulfate, sodium 7-ethyl-2-methyl-4-undecyl sulfate and sodium 2-ethylhexyl sulfate, especially sodium dodecyl sulfate.
  • Cs-Cis alkyl sulfates such as sodium dodecyl sulfate, lithium dodecyl sulfate, ammonium dodecyl sulfate, sodium tetradecyl sulfate, sodium 7-ethyl-2-methyl-4-undecyl sulfate and sodium 2-ethylhexyl sulfate, especially sodium dodecyl s
  • Suitable alkyl ether sulfates include, but are not limited to, C 8 -Ci 8 alkyl ether sulfates such as sodium laureth sulfate and sodium myreth sulfate.
  • Suitable alkyl sulfonates or sulfonic acids include, but are not limited to, Cg-Cig alkyl sulfonates such as sodium tetradecyl sulfonate, sodium octadecyl sulfonate, sodium dodecyl sulfonate, sodium hexadecyl sulfonate and the corresponding sulfonic acids.
  • Suitable aryl sulfonates or sulfonic acids optionally substituted with alkyl or aryl substituents include, but are not limited to, Cs-Cis alkylbenzene sulfonates or sulfonic acids such as sodium dodecylbenzene sulfonate, dodecylbenzene sulfonic acid and dodecylbenzene sulfonic acid isopropylamine salt; C4-C1 8 alkylnaphthalene sulfonates or sulfonic acids such as sodium butylnaphthalene sulfonate and sodium hexylnaphthalene sulfonate; and C 6 -Ci 6 alkyl diphenyloxide disulfonates or disulfonic acids such as sodium dodecyl diphenyloxide disulfonate; especially sodium dodecylbenzene sulfonate or dodecylbenz
  • the aryl sulfonate or sulfonic acid may be positioned at any point along the alkyl chain, for example on a primary, secondary or tertiary carbon.
  • Suitable alkyl ester sulfonates or sulfonic acids include, but are not limited to, Cs-Cis alkyl methyl ester sulfonates or sulfonic acids such as methyl ester sulfonate, sodium dodecyl methyl ester a-sulfonate, sodium tetradecyl methyl ester a-sulfonate and sodium hexadecyl methyl ester a-sulfonate.
  • the sulfate, sulfonate or sulfonic acid groups may be positioned at any point along the alkyl chain or aryl ring, for example on a primary, secondary or tertiary carbon.
  • Each of the anionic surfactants may contain a mixture of compounds varying in the length of the alkyl chain.
  • one surfactant is used. In other embodiments, more than one surfactant is used, for example, two or three surfactants may be used together.
  • Suitable optional substituents include, but are not limited to, alkyl groups such as C4-C18 alkyl and aryl groups such as phenyl, naphthyl, tetrahydronaphthyl, biphenyl and binaphthyl.
  • Suitable salts include, but are not limited to, those formed with cations such as sodium, potassium, lithium, ammonium and isopropylamine; especially sodium.
  • the amount of surfactant in the composition may depend on the treatment site and volume of sewage to be treated.
  • the amount of surfactant is optimised to prevent over foaming or loss of activity.
  • the surfactant in the composition is in an amount in the range of from 0.1% to 2% w/w, 0.3% to 1.7% w/w, 0.5% to 1.5% w/w, especially 0.8% to 1.2% w/w, more especially 0.9% to 1.1% w/w, most especially about 1% w/w.
  • the composition for use in the methods of the invention comprises at least one microorganism of the genus Bacillus.
  • the microorganism is in a vegetative state.
  • the microorganism is in a dormant state, for example, an endospore.
  • the microorganism is present in a mixture of dormant and vegetative states.
  • the microorganism of the genus Bacillus may be selected from Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus pumilus, Bacillus popilliae, Bacillus circulans and combinations thereof; especially Bacillus subtilis and Bacillus licheniformis; more especially Bacillus subtilis.
  • the at least one microorganism of the genus Bacillus is a specific strain of Bacillus subtilis, especially Bacillus subtilis NRRL B-3383 (US Department of Agriculture, Agricultural Research Service, ARS Culture Collection NRRL), B. subtilis ATCC 21331, B. subtilis ATCC 21332, B. subtilis SD901 (FERM BP.7666), B. subtilis ATCC 6051, B. subtilis RSA-203 or combinations thereof; especially B. subtilis RSA-203.
  • B. subtilis RSA-203 is a rod-shaped, aerobic, Gram positive, ⁇ -haemolytic microbe capable of forming endospores. Nucleic acid sequence analysis confirms that it is a strain of B. subtilis. A sample of this microorganism was deposited at the ATCC depository, 10801 University Boulevard, Manassas, Virginia 20110-2209, United States of America on 9 January 2013 and has been allocated Accession No. PTA-13451.
  • the composition comprises more than one microorganism from the genus Bacillus.
  • the amount of microorganism of the genus Bacillus is an amount sufficient to achieve an effective population at the site of application.
  • the microorganism of the genus Bacillus is present in the composition in an amount in the range of from 1 x 10 4 cfu/mL to 1 x 10 7 cfu/mL, 5 x 10 4 cfu/mL to 7.5 x 10 6 cfu/mL, 1 x 10 5 cfu/mL to 5 x 10 6 cfu/mL, 5 x 10 5 cfu/mL to 2.5 x 10 6 cfu/mL, especially 7.5 x 10 5 cfu/mL to 2 x 10 6 cfu/mL, most especially 1 x 10 6 cfu/mL or 1.2 x 10 6 cfu/mL.
  • the composition useful in the invention may be applied to a wet well, including, but not limited to, the surface of the sewage in the wet well or to the wet well wall.
  • the composition useful in the invention may be applied as a foam.
  • the composition may be applied directly to the surface of the sewage, may be applied directly to the surface of the sewage in the wet well or may be applied to the wet well wall, especially as a foam.
  • the composition may be added as a bolus.
  • the composition may be added continuously at a rate suitable to maintain contact with the sewage and/or surface of the sewage transport system.
  • the composition is added as a bolus.
  • the sewage is not further mixed after addition of the composition to maintain a high local concentration of the composition.
  • the composition is applied at the commencement of the fill cycle in the wet well.
  • the composition of the invention is applied as a foam.
  • the foam has a long residence time at the surface of the sewage or on surfaces in the sewage transport system.
  • the foam may be present for greater than 15 minutes, especially greater than 30 minutes, most especially greater than one hour.
  • the formulation of the composition as a foam is thought to maintain a high local concentration of the composition at the point of application, for example on the surface of the sewage or on the surfaces of the sewage transport system, particularly on the surfaces of the wet well, such that the composition is added in a phase specific manner.
  • This is thought to enable a prolonged contact time between the composition of the invention and the odour and corrosion causing microorganisms and, therefore, a lower effective dosage rate.
  • a prolonged foam residence time is advantageous for such activity.
  • the foam may be generated prior to application to the sewage transport system or may be generated during the application process.
  • the foam may be generated by spraying, misting, infusing with gas or air, stirring, using a spray or jet head or a foam dispenser.
  • composition of the invention is applied to the sewage transport system by spraying, pouring, hosing, misting or dripping; especially spraying or misting.
  • Sewage has a variable composition which depends on the substances being discharged into the sewage transport system.
  • sewage comprises carbonaceous and nitrogenous waste matter and populations of many different microbes.
  • the flow rate of sewage in the sewage transport system also varies, which determines the retention time of the sewage in the sewage transport system. For example, the flow rate of sewage is slower at night time compared with daytime and is faster in the wet weather compared with the dry weather.
  • Treatment regimes may be established for different sites of application by a person skilled in the art. Optimal concentrations of the composition will be required to maintain activity and maintain sufficient foaming whilst avoiding over foaming.
  • the dosage rate of the composition will be a dose sufficient to maintain a suitable local concentration of the composition at the site of application.
  • the sewage flow rate, the sewage volume to be treated and environmental factors will also be taken into account when determining the dosage rate of the composition.
  • Suitable dosage rates of the compositions of the invention may include 1:1000 to 1:1000000 parts relative to sewage flow, 1:5000 to 1:500000 parts relative to sewage flow, 1:7500 to 1:250000 parts relative to sewage flow, especially 1:10000 to 1:100000 parts relative to sewage flow or 1:25000 to 1:75000 parts relative to sewage flow, most especially 1:50000 parts relative to sewage flow.
  • biofilm formation and/or adhesion on surfaces of the sewage transport system is reduced and/or prevented.
  • biofilms on surfaces of the sewage transport system are disrupted. Said biofilms may have been formed prior to treatment with a composition of the invention.
  • Sulfur and/or sulfate reducing bacteria may be present in biofilms on surfaces in the sewage transport system.
  • the use of compositions of the present invention to prevent or reduce biofilm formation and/or adhesion and/or to disrupt existing biofilms will assist in the reduction of malodourous hydrogen sulfide and other malodourous gases in the sewage transport system.
  • the reduction of hydrogen sulfide in the sewage transport system may also reduce the amount of sulfuric acids and sulfates, therefore reducing the corrosion of surfaces in the sewage transport system.
  • Microbial populations that may be affected by the methods of the invention may include Gram positive and Gram negative bacteria, autotrophic bacteria, heterotrophic bacteria, nitrogen-fixing bacteria and methanogenic archaea that are present in sewage.
  • the methods of the invention may also affect populations of aerobic, facultative anaerobic and anaerobic bacteria, and are particularly useful against strains of bacteria that produce malodourous gas such as hydrogen sulfide gas, and bacteria that convert sulfide to sulfate.
  • the methods of the invention are also useful against ammonia forming, nitrite forming, nitrate forming, denitrifying, methane forming, metal reducing such as iron reducing and acid producing bacteria.
  • the methods of the invention are active against sulfur and/or sulfate reducing bacteria.
  • affected bacteria may include, but is not limited to, sulfur and/or sulfate reducing bacteria such as Desulfovibrio species, particularly Desulfovibrio de ulfuric ans, Desulfovibrio salixigens, Desulfovibrio africanus and Desulfovibrio vulgaris; Desulfotomaculum species particularly Desulfotomaculum nigrificans and Desulfotomaculum halophilum; Desulfosporosinus species such as Desulfosporosinus orientis; Desulfosporomusa species such as Desulfosporomusa polytropa; sulfide oxidising bacteria such as Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans, Thiobacillus concretivorous, Thiobacillus intermedins and Thiobacillus thioparus; bacteria from the Enterobacteriaceae family such as Pantoea agglomerans, Enterobacter cloaca
  • a method of reducing odour in a sewage transport system comprising addition of a composition of the invention to the sewage transport system.
  • odour is reduced through microbial control, mainly through the direct reduction of biological activity and the resultant increase in dissolved oxygen levels, which further suppresses anaerobic microbial activity.
  • hydrogen sulfide production in the sewage transport system is reduced by application of a composition of the invention.
  • a composition of the invention it is thought that hydrogen sulfide production is reduced through the control of sulfur or sulfate reducing bacteria in the sewage transport system.
  • the amount of hydrogen sulfide in the sewage transport system may be reduced to an amount below 10 ppm, 7 ppm, 5 ppm, 3 ppm or 1 ppm.
  • corrosion of surfaces in the sewage transport system is reduced and/or prevented. Any surface in the sewage transport system may be susceptible to corrosion, especially either concrete or metal surfaces. The corrosion may occur at any point in the sewage transport system, particularly in the pipes, wet wells and/or hydraulic sections such as the pumps.
  • the anionic surfactant in the composition may have some foaming activity, in some embodiments, the composition for use in the methods of the invention further comprises a foaming agent.
  • Suitable foaming agents may include non-ionic surfactants, such as alkyl and polyalkyl esters of poly (ethylene oxide), alkyl and polyalkyl ethers of poly(ethylene oxide), alkyl and polyalkyl glycosides or polyglycosides in particular alkyl and polyalkyl glucosides or polyglucosides and alkylalkanolamides; zwitterionic surfactants such as alkylamidopropyl betaines, for example cocamidopropyl betaine and lauramidopropyl betaine; and combinations thereof.
  • non-ionic surfactants such as alkyl and polyalkyl esters of poly (ethylene oxide), alkyl and polyalkyl ethers of poly(ethylene oxide), alkyl and polyalkyl glycosides or polyglycosides in particular alkyl and polyalkyl glucosides or polyglucosides and alkylalkanolamides
  • zwitterionic surfactants such as al
  • the foaming agent is a non-ionic surfactant selected from an alkylmonoethanolamide or an alkyldiethanolamide, for example a Cs-Cis alkylmonoethanolamide such as cocomonoethanolamide or a Cs-Cis alkyldiethanolamide such as cocodiethanolamide; most especially cocomonoethanolamide.
  • each of these foaming agents may contain a mixture of compounds varying in the length of the alkyl chain. The amount of foaming agent in the composition depends on the treatment site and volume of sewage to be treated.
  • the amount of foaming agent is optimised to prevent over foaming and leakage of the foam from the sewage transport system, but still maintain an effective amount of foam at the surface of the application site of the sewage transport system, such as a wet well.
  • the foam formed is a stable foam and may maintain structural integrity for an extended period of time, for example for greater than 15 minutes, especially greater than 30 minutes, most especially greater than one hour.
  • the foaming agent in the composition is in an amount in the range of from 0.1% to 1% w/w, especially about 0.5% w/w.
  • the composition for use in the methods of the invention further comprises a thickening agent.
  • Suitable thickening agents include, but are not limited to, natural gums such as xanthan gum, guar gum, gum arabic, carrageenan, locust bean gum, alginate, karaya gum, tragacanth, curdlan, scleroglucan, gellan gum, gum ghatti, beta- glucans, chicle gum, dammar gum, glucomannan, mastic gum, tara gum and agar; carboxymethyl cellulose; hydroxypropylmethyl cellulose; hydroxyethyl cellulose; hydroxypropyl cellulose; ethyl cellulose; methyl cellulose; carboxymethylhydroxyethyl cellulose; pectin; starch; gelatin; carbomers; fumed silica; hydroxypropyl guar gum; polyethylene glycols; and combinations thereof.
  • the thickening agent may be present in the composition in an amount in the range of from 0.1% to 1% w/w, especially about 0.3% w/w.
  • the composition for use in the methods of the invention further comprises a biosurfactant.
  • Suitable biosurfactants may include, but are not limited to, antimicrobial cyclic lipopeptide biosurfactants such as surfactin, lichenysin, iturin, fengycin, bacillomycin, mycosubtilin and combinations thereof; especially surfactin.
  • Each of these biosurfactants may contain mixtures of compounds, varying in chain length of the fatty acid moiety of the lipopeptide.
  • the biosurfactant is produced by the microorganism of the genus Bacillus that is in the composition.
  • the biosurfactant may be produced by the microorganism during production of the microorganism for inclusion in the composition.
  • the microorganism and biosurfactant are produced together and included in the composition without isolation from one another.
  • the biosurfactant is added to the composition during manufacture.
  • the biosurfactant is not produced by the microorganism of the genus Bacillus that is in the composition.
  • the biosurfactant is added to the composition during manufacture.
  • the biosurfactant may be present in the composition in an amount in the range of from 0.001% to 0.1% w/w, 0.003% to 0.05% w/w, 0.004% to 0.04% w/w, 0.005% to 0.03% w/w, 0.006% to 0.02%, especially about 0.01% w/w or about 0.0067% w/w.
  • the composition does not contain an added biosurfactant.
  • compositions useful in the invention may also comprise additional components produced by the microorganism of the genus Bacillus that is in the composition. These components may be produced by the microorganism during production of the microorganism for inclusion in the composition.
  • the composition may further comprise one or more isocoumarins such as amicoumacin A, B and C, and/or other low molecular weight precursors for biosurfactants (i.e. components with a molecular weight less than 700 Da). These components may be added to the composition without isolation from the microorganism. In other embodiments, these components are not produced by the microorganism of the genus Bacillus that is in the composition and may be added to the composition during manufacture.
  • the composition for use in the methods of the invention comprises a preservative.
  • Suitable preservatives include, but are not limited to, sodium benzoate, benzoic acid, sorbic acid, lactic acid, nitrites such as sodium nitrite, nitrates such as sodium nitrate, propionic acid, sodium propionate, sodium sorbate, ascorbic acid and combinations thereof; especially sodium benzoate.
  • the preservative may be present in the composition in an amount in the range of from 0.03% to 0.6% w/w or 0.1% to 0.4% w/w, especially about 0.3% w/w or about 0.2% w/w.
  • the composition for use in the methods of the invention comprises a liquid medium.
  • suitable liquid mediums include, but are not limited to, aqueous carriers such as water, buffer, culture medium, culture medium used to culture the microorganism and combinations thereof.
  • the composition comprises the culture medium containing the microorganism in a sufficient quantity produced directly from the culture process.
  • Suitable buffers include, but are not limited to, citric acid and phosphate such as monosodium phosphate, monopotassium phosphate, disodium phosphate and dipotassium phosphate.
  • the pH of the composition is in the range of from pH 4 to 10, especially pH 5 to 9, most especially pH 6 to 8.
  • Exemplary culture medium may comprise aqueous monopotassium phosphate, disodium phosphate, magnesium sulfate heptahydrate, calcium chloride hexahydrate, disodium EDTA dehydrate, ferrous sulfate heptahydrate, manganese sulfate monohydrate, ammonium chloride, sodium nitrate, glucose monohydrate and yeast extract.
  • composition comprising at least one microorganism of the genus Bacillus in an amount in the range of from 1 x 10 4 cfu/mL to 1 x 10 7 cfu/mL and one or more anionic surfactants selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ether sulfonates or sulfonic acids and salts thereof in an amount in the range of from 0.1 % to 2% w/w.
  • anionic surfactants selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ether s
  • the amount of microorganism of the genus Bacillus is an amount sufficient to achieve an effective population at the site of application.
  • the microorganism of the genus Bacillus is in an amount in the range of from 1 x 10 4 cfu/mL to 1 x 10 7 cfu/mL, 5 x 10 4 cfu/mL to 7.5 x 10 6 cfu/mL, 1 x 10 5 cfu/mL to 5 x 10 6 cfu/mL, 5 x 10 5 cfu/mL to 2.5 x 10 6 cfu/mL, especially 7.5 x 10 5 cfu/mL to 2 x 10 6 cfu/mL, most especially 1 x 10 6 cfu/mL or 1.2 x 10 6 cfu/mL.
  • the microorganism is in a vegetative state. In other embodiments, the microorganism is in a dormant state, for example, an endospore. In further embodiments, the microorganism is present in a mixture of dormant and vegetative states.
  • the microorganism of the genus Bacillus may be selected from Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus pumilus, Bacillus popilliae, Bacillus circulans and combinations thereof; especially Bacillus subtilis and Bacillus licheniformis; more especially Bacillus subtilis.
  • the at least one microorganism of the genus Bacillus is a specific strain of Bacillus subtilis, especially Bacillus subtilis NRRL B-3383 (US Department of Agriculture, Agricultural Research Service, ARS Culture Collection NRRL), B. subtilis ATCC 21331, B. subtilis ATCC 21332, B. subtilis SD901 (FERM BP.7666), B. subtilis ATCC 6051, B. subtilis RSA-203 or combinations thereof; especially B. subtilis RSA-203.
  • B. subtilis RSA-203 is a rod-shaped, aerobic, Gram positive, ⁇ -haemolytic microbe capable of forming endospores. Nucleic acid sequence analysis confirms that it is a strain of B. subtilis. A sample of this microorganism was deposited at the ATCC depository, 10801 University Boulevard, Manassas, Virginia 20110-2209, United States of America on 9 January 2013 and has been allocated Accession No. PTA-13451.
  • the composition comprises more than one microorganism from the genus Bacillus.
  • the surfactant is an anionic surfactant selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ester sulfonates or sulfonic acids and salts and mixtures thereof.
  • Suitable alkyl sulfates include, but are not limited to, Cs-Cis alkyl sulfates such as sodium dodecyl sulfate, lithium dodecyl sulfate, ammonium dodecyl sulfate, sodium tetradecyl sulfate, sodium 7-ethyl-2-methyl-4-undecyl sulfate and sodium 2-ethylhexyl sulfate, especially sodium dodecyl sulfate.
  • Cs-Cis alkyl sulfates such as sodium dodecyl sulfate, lithium dodecyl sulfate, ammonium dodecyl sulfate, sodium tetradecyl sulfate, sodium 7-ethyl-2-methyl-4-undecyl sulfate and sodium 2-ethylhexyl sulfate, especially sodium dodecyl s
  • Suitable alkyl ether sulfates include, but are not limited to, C 8 -Ci 8 alkyl ether sulfates such as sodium laureth sulfate and sodium myreth sulfate.
  • Suitable alkyl sulfonates or sulfonic acids include, but are not limited to, Cs-Cis alkyl sulfonates such as sodium tetradecyl sulfonate, sodium octadecyl sulfonate, sodium dodecyl sulfonate, sodium hexadecyl sulfonate and the corresponding sulfonic acids.
  • Suitable aryl sulfonates or sulfonic acids optionally substituted with alkyl or aryl substituents include, but are not limited to, Cs-Cis alkylbenzene sulfonates or sulfonic acids such as sodium dodecylbenzene sulfonate, dodecylbenzene sulfonic acid and dodecylbenzene sulfonic acid isopropylamine salt; C4-C1 8 alkylnaphthalene sulfonates or sulfonic acids such as sodium butylnaphthalene sulfonate and sodium hexylnaphthalene sulfonate; and C 6 -Ci 6 alkyl diphenyloxide disulfonates or disulfonic acids such as dodecyl diphenyloxide disulfonate; especially sodium dodecylbenzene sulfonate or dodecylbenzen
  • the aryl sulfonate or sulfonic acid may be positioned at any point along the alkyl chain, for example on a primary, secondary or tertiary carbon.
  • Suitable alkyl ester sulfonates or sulfonic acids include, but are not limited to, Cs-Cis alkyl methyl ester sulfonates or sulfonic acids such as methyl ester sulfonate, sodium dodecyl methyl ester a-sulfonate, sodium tetradecyl methyl ester a-sulfonate and sodium hexadecyl methyl ester a-sulfonate.
  • the sulfate, sulfonate or sulfonic acid groups may be positioned at any point along the alkyl chain or aryl ring, for example on a primary, secondary or tertiary carbon.
  • Each of the anionic surfactants may contain a mixture of compounds varying in the length of the alkyl chain.
  • the composition comprises one surfactant. In other embodiments, the composition comprises more than one surfactant, for example, two or three surfactants.
  • Suitable optional substituents include, but are not limited to, alkyl groups such as C4-C18 alkyl and aryl groups such as phenyl, naphthyl, tetrahydronaphthyl, biphenyl and binaphthyl.
  • Suitable salts include, but are not limited to, those formed with cations such as sodium, potassium, lithium, ammonium and isopropylamine; especially sodium.
  • the amount of surfactant in the composition may depend on the treatment site and volume of sewage to be treated.
  • the surfactant in the composition is in an amount in the range of from 0.1% to 2% w/w, 0.3% to 1.7% w/w, 0.5% to 1.5% w/w, especially 0.8% to 1.2% w/w, more especially 0.9% to 1.1% w/w, most especially about 1% w/w.
  • the anionic surfactant in the composition may have some foaming activity, in some embodiments, the composition further comprises a foaming agent.
  • Suitable foaming agents may include, but are not limited to, non-ionic surfactants, such as alkyl and poly alkyl esters of poly (ethylene oxide), alkyl and poly alkyl ethers of poly (ethylene oxide), alkyl and polyalkyl glycosides or polyglycosides in particular alkyl and polyalkyl glucosides or polyglucosides and alkylalkanolamides; zwitterionic surfactants such as alkylamidopropyl betaines, for example cocamidopropyl betaine and lauramidopropyl betaine; and combinations thereof.
  • non-ionic surfactants such as alkyl and poly alkyl esters of poly (ethylene oxide), alkyl and poly alkyl ethers of poly (ethylene oxide), alkyl and polyalkyl glycosides or polyglycosides in particular alkyl and polyalkyl glucosides or polyglucosides and alkylalkanolamides; zwitterionic
  • the non-ionic surfactant is an alkylmonoethanolamide or an alkyldiethanolamide, for example a Cs-Cis alkylmonoethanolamide such as cocomonoethanolamide or a Cs-Cis alkyldiethanolamide such as cocodiethanolamide; most especially cocomonoethanolamide.
  • each of these foaming agents may contain a mixture of compounds varying in the length of the alkyl chain.
  • the amount of foaming agent in the composition depends on the treatment site and volume of sewage to be treated.
  • the composition forms a stable foam and may maintain structural integrity for an extended period of time, for example for greater than 15 minutes, especially greater than 30 minutes, most especially greater than one hour.
  • the foaming agent in the composition is in an amount in the range of from 0.1% to 1% w/w, especially about 0.5% w/w.
  • the composition further comprises a thickening agent.
  • Suitable thickening agents include, but are not limited to, natural gums such as xanthan gum, guar gum, gum arabic, carrageenan, locust bean gum, alginate, karaya gum, tragacanth, curdlan, scleroglucan, gellan gum, gum ghatti, beta-glucans, chicle gum, dammar gum, glucomannan, mastic gum, tara gum and agar; carboxymethyl cellulose; hydroxypropylmethyl cellulose; hydroxyethyl cellulose; hydroxypropyl cellulose; ethyl cellulose; methyl cellulose; carboxymethylhydroxyethyl cellulose; pectin; starch; gelatin; carbomers; fumed silica; hydroxypropyl guar gum; polyethylene glycols; and combinations thereof.
  • the thickening agent may be present in the composition in an amount in the range of from 0.1% to 1% w/w, especially about 0.3% w/w.
  • the composition further comprises a biosurfactant.
  • Suitable biosurfactants may include, but are not limited to, antimicrobial cyclic lipopeptide biosurfactants such as surfactin, lichenysin, iturin, fengycin, bacillomycin, mycosubtilin and combinations thereof; especially surfactin.
  • Each of these biosurfactants may contain mixtures of compounds, varying in chain length of the fatty acid moiety of the lipopeptide.
  • the biosurfactant is produced by the microorganism of the genus Bacillus that is in the composition.
  • the biosurfactant may be produced by the microorganism during production of the microorganism for inclusion in the composition.
  • the microorganism and biosurfactant are produced together and included in the composition without isolation from one another.
  • the biosurfactant is added to the composition during manufacture.
  • the biosurfactant is not produced by the microorganism of the genus Bacillus that is in the composition. In these embodiments, the biosurfactant is added to the composition during manufacture.
  • the biosurfactant may be present in the composition in an amount in the range of from 0.001% to 0.1% w/w, 0.003% to 0.05% w/w, 0.004% to 0.04% w/w, 0.005% to 0.03% w/w, 0.006% to 0.02%, especially about 0.01% w/w or about 0.0067% w/w.
  • the composition does not contain an added biosurfactant.
  • compositions of the invention may also comprise additional components produced by the microorganism of the genus Bacillus that is in the composition. These components may be produced by the microorganism during production of the microorganism for inclusion in the composition.
  • the composition may further comprise one or more isocoumarins such as amicoumacin A, B and C, and/or other low molecular weight precursors for biosurfactants (i.e. components with a molecular weight less than 700 Da). These components may be added to the composition without isolation from the microorganism. In other embodiments, these components are not produced by the microorganism of the genus Bacillus that is in the composition and may be added to the composition during manufacture. In some embodiments, the composition further comprises a preservative.
  • Suitable preservatives include, but are not limited to, sodium benzoate, benzoic acid, sorbic acid, lactic acid, nitrites such as sodium nitrite, nitrates such as sodium nitrate, propionic acid, sodium propionate, sodium sorbate, ascorbic acid and combinations thereof; especially sodium benzoate.
  • the preservative may be present in the composition in an amount in the range of from 0.03% to 0.6% w/w or 0.1% to 0.4% w/w, especially about 0.3% w/w or about 0.2% w/w.
  • the composition comprises a liquid medium.
  • suitable liquid mediums include, but are not limited to, aqueous carriers such as water, buffer, culture medium, culture medium used to culture the microorganism and combinations thereof.
  • the composition comprises the culture medium containing the microorganism in a sufficient quantity produced directly from the culture process.
  • Suitable buffers include, but are not limited to, citric acid and phosphate such as monosodium phosphate, monopotassium phosphate, disodium phosphate and dipotassium phosphate.
  • the pH of the composition is in the range of from pH 4 to 10, especially pH 5 to 9, most especially pH 6 to 8.
  • Exemplary culture medium may comprise aqueous monopotassium phosphate, disodium phosphate, magnesium sulfate heptahydrate, calcium chloride hexahydrate, disodium ethylenediaminetetraacetic acid (EDTA) dehydrate, ferrous sulfate heptahydrate, manganese sulfate monohydrate, ammonium chloride, sodium nitrate, glucose monohydrate and yeast extract.
  • EDTA disodium ethylenediaminetetraacetic acid
  • the composition may be formulated as a concentrate which may be diluted before use. In other embodiments, the composition does not require dilution before use.
  • the composition is formulated as a foam.
  • the foam may be formed from the diluted or concentrated composition.
  • composition of the invention may be prepared by combining the one or more anionic surfactants selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ester sulfonates or sulfonic acids and salts or mixtures thereof with the liquid medium.
  • Foaming agents, thickening agents, biosurfactants and/or preservatives are also added to the liquid medium, if required.
  • the cultured microorganism of the genus Bacillus is then added to the composition, particularly in endospore form.
  • the composition of the invention may be prepared by combining culture medium comprising the cultured microorganism of the genus Bacillus, particularly in endospore form, with an aqueous carrier comprising one or more anionic surfactants selected from alkyl sulfates, alkyl ether sulfates, alkyl sulfonates or sulfonic acids, aryl sulfonates or sulfonic acids optionally with alkyl or aryl substituents, alkyl ester sulfonates or sulfonic acids and salts thereof, and, if required, foaming agents, thickening agents, biosurfactants and/or preservatives.
  • the culture medium may be the medium used to culture the microorganism of the genus Bacillus.
  • compositions of the Examples are readily available from commercial sources, such as Sigma-Aldrich Co. LLC. Cocomonoethanolamide is available from, for example, Alpha Chemicals Pvt Ltd. Bacterial species are commercially available from commercial sources, such as American Type Culture Collection (ATCC). Pseudomonas baetica is available from, for example, National Collection of Industrial, Food and Marine Bacteria (NCIMB) Culture Collection.
  • ATCC American Type Culture Collection
  • NCIMB National Collection of Industrial, Food and Marine Bacteria
  • a composition of the invention was prepared by combining 1 % w/w (final concentration) sodium dodecylbenzene sulfonate, 0.3% w/w (final concentration) xanthan gum, 0.5% w/w (final concentration) cocomonoethanolamide, 0.01% w/w (final concentration) surfactin and spent culture medium (aqueous monopotassium phosphate, disodium phosphate, magnesium sulfate heptahydrate, calcium chloride hexahydrate, disodium EDTA dehydrate, ferrous sulfate heptahydrate, manganese sulfate monohydrate, ammonium chloride, sodium nitrate and traces of glucose monohydrate and yeast extract) comprising B. subtilis endospores (final concentration of 1 x 10 6 cfu/mL) in a 1000 gallon stainless steel dilution reactor with slight agitation.
  • B. subtilis endospores final concentration of 1 x 10 6 cfu/m
  • a composition of the invention was prepared by combining 1 % w/w (final concentration) sodium dodecylbenzene sulfonate, 0.01% w/w (final concentration) surfactin and spent culture medium (aqueous monopotassium phosphate, disodium phosphate, magnesium sulfate heptahydrate, calcium chloride hexahydrate, disodium EDTA dehydrate, ferrous sulfate heptahydrate, manganese sulfate monohydrate, ammonium chloride, sodium nitrate and traces of glucose monohydrate and yeast extract) comprising B. subtilis endospores (final concentration of 1 x 10 6 cfu/mL) in a 1000 gallon stainless steel dilution reactor with slight agitation.
  • Example 3 Activity of compositions against bacteria
  • compositions of the invention were determined using a bacterial growth inhibition assay.
  • the bacterial species tested are representative of bacterial species typically present in a sewage transport system.
  • Composition 1 (1% w/w dodecylbenzene sulfonic acid, 0.01% surfactin, 1 x 10 6 B. subtilis endospores in spent culture medium, pH 4.5-5.5; prepared by the method of Example 2 using dodecylbenzene sulfonic acid) was added (in duplicate) to cultures containing 1 x 10 5 cells of the test bacterium (Desulfovibrio desulfuricans, Enterobacter cloacae, Rahnella aquatilis, Pseudomonas baetica, Enterobacteriaceae bacterium, Pantoea agglomerans and Shewanella oneidensis) in culture medium.
  • test bacterium Desulfovibrio desulfuricans, Enterobacter cloacae, Rahnella aquatilis, Pseudomonas baetica, Enterobacteriaceae bacterium, Pantoea agglomerans and Shewanella onei
  • the final concentration of Composition 1 in the culture medium was 250 ppm, 500 ppm and 1000 ppm.
  • the cultures were incubated at 30-37° C.
  • the optical density at 600 nm (OD 600 nm) of the cultures were measured to determine the growth of the bacteria ( Figures 1 to 7).
  • Composition 1 inhibited the growth of D. desulfuricans for up to 170 hours at a concentration of 1000 ppm, 78 hours at a concentration of 500 ppm and 46 hours at a concentration of 250 ppm ( Figure 1).
  • Composition 1 inhibited the growth of R. aquatilis for up to 145 hours at all concentrations tested ( Figure 3).
  • Composition 1 inhibited the growth of P. agglomerans for up to 121 hours at a concentration of 1000 ppm. At 500 ppm of Composition 1, growth was inhibited for 50 hours. A slight inhibition of P. agglomerans growth was observed in the presence of 250 ppm of Composition 1 ( Figure 6).
  • Composition 1 inhibits the growth of bacterial species typically present in a sewage transport system and, therefore, Composition 1 is expected to be effective in reducing or preventing biofilm formation, reducing hydrogen sulfide production or reducing corrosion in a sewage transport system.
  • compositions of the invention comprising 1.2 x 10 6 cfu/mL B. subtilis endospores in spent culture medium, 0.0067% w/w surfactin, 1% w/w sodium dodecylbenzene sulfonate and 0.2% w/w sodium benzoate disrupt biofilms and reduce hydrogen sulfide levels in a sewage transport system.
  • composition 2 comprised 1.2 x 10 6 cfu/mL B. subtilis endospores in spent culture medium, 0.0067% w/w surfactin, 1% w/w sodium dodecylbenzene sulfonate and 0.2% w/w sodium benzoate and was produced in accordance with the method of Example 2.
  • Composition 3 comprised aqueous 1% sodium dodecylbenzene sulfonate (pH adjusted to 7 with sodium hydroxide).
  • Table 1 L. monocytogenes growth in the presence of Compositions 2 and 3.
  • composition 2 At their highest concentrations, application of Composition 2 caused a log 3.75 reduction in the number of L. monocytogenes colonies, whereas Composition 3 caused a log 2.20 reduction. It is evident from the data above that the composition comprising sodium dodecylbenzene sulfonate alone (Composition 3) is less effective at inhibiting L. monocytogenes growth than the composition comprising B. subtilis endospores in spent culture medium, surfactin, sodium dodecylbenzene sulfonate and sodium benzoate (Composition 2). Composition 2 effectively inhibited L. monocytogenes growth, particularly at higher concentrations.

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Abstract

La présente invention concerne des procédés de traitement d'un système de transport d'eaux usées, comprenant la réduction des odeurs, la réduction de la production de sulfure d'hydrogène, la réduction et/ou la prévention de la corrosion, la réduction et/ou la prévention de la formation des biofilms et/ou de leur adhérence et la dislocation des biofilms. Des compositions appropriées pour être utilisées dans de tels procédés, comprenant un ou plusieurs tensioactifs anioniques choisis parmi les sulfates d'alkyle, les sulfates d'éther d'alkyle, les acides sulfoniques ou sulfonates d'alkyle, les acides sulfoniques ou sulfonates d'aryle éventuellement avec substituants alkyle ou aryle, les acides sulfoniques ou sulfonates d'ester d'alkyle et leurs sels, de préférence avec au moins un micro-organisme du genre Bacillus, sont également décrites.
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CN109928519A (zh) * 2019-04-20 2019-06-25 无锡吉致电子科技有限公司 一种高效水体净化剂的制备方法
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WO2021062489A1 (fr) * 2019-10-04 2021-04-08 Global Bioprotect Ip Pty Ltd Système et procédé de production d'un biotensioactif et formulations le comprenant
CN112961807A (zh) * 2021-03-30 2021-06-15 中国科学院成都生物研究所 一种微生物组合物及在促进青稞种子发芽和生长上的应用
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