WO2016088061A1 - 一种细胞电转仪 - Google Patents

一种细胞电转仪 Download PDF

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Publication number
WO2016088061A1
WO2016088061A1 PCT/IB2015/059296 IB2015059296W WO2016088061A1 WO 2016088061 A1 WO2016088061 A1 WO 2016088061A1 IB 2015059296 W IB2015059296 W IB 2015059296W WO 2016088061 A1 WO2016088061 A1 WO 2016088061A1
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Prior art keywords
electrode terminal
electric shock
shock tube
cell
control unit
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PCT/IB2015/059296
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English (en)
French (fr)
Inventor
陈剑
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陈剑
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Publication of WO2016088061A1 publication Critical patent/WO2016088061A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control

Definitions

  • the invention belongs to the technical field of biomedical instruments and relates to a cell electrophoresis instrument. Background technique
  • Cell electroporation also known as cell electroporation or cell electroporation
  • Electroporation is a widely used and highly recommended method for cell testing and gene therapy. When a strong electric field is applied, the cell membrane temporarily becomes porous and can pass through foreign materials such as macromolecules. Cell membrane electrical penetration depends on various parameters of the electric field, such as pulse type, pulse voltage, pulse duration, number of pulses, and other experimental conditions.
  • the devices currently used for cell electroporation mainly include cell electrosurgical transducers, electrode cups, and the like.
  • China Patent Publication No.: CN1965079B, Publication Date: 2007-05-16 discloses an electroporation device having a long hollow member, the electroporation device comprising a long hollow member for providing a uniform electric field during electroporation.
  • performing electroporation is by applying an electrical pulse from both ends of the long hollow member by a pair of electrodes after the hollow member is filled with a liquid sample comprising the cells and the substance to be injected into the cells.
  • the applicant designs an annular groove in the wall of the electric shock tube that communicates with the cavity of the electric shock tube.
  • the experimenter can continuously inject a liquid sample into the cavity of the electric shock tube until the surface of the liquid protrudes from the electric shock tube cavity.
  • the upper electrode cover is brought into contact with the liquid convex surface until it is pressed to the upper edge of the electric shock tube cavity to form a seal for the liquid inside the cavity, and a small amount of overflowed liquid flows into the annular groove.
  • This design generally prevents residual air in the electric shock tube from affecting the experiment.
  • the invention provides a cell electro-rotation instrument, and the technical problem to be solved by the invention is: how to cooperate with the electric shock tube to realize stable electrorotation treatment of the cell sample.
  • a cell electro-rotation device comprising: a casing, a power module, and a micro-control unit, wherein the micro-control unit and the power module are electrically coupled, wherein the cell electrical converter is provided with a fixing seat, A socket for fixing the electric shock tube is disposed on the fixing base, and the electrode terminal and the electrode terminal 2 electrically connected to the power module are further disposed in the housing, and the electrode terminal 1 and the electrode terminal 2 are respectively used Supplying power on two electrodes of the electric shock tube, and a spring for pressing the electrode terminal 1 and/or the electrode terminal 2 on the two electrodes of the electric shock tube is further provided in the casing, the micro The control unit is capable of receiving user commands and performing control on the power modules.
  • the electric shock tube is a tubular device capable of holding a liquid sample inside and can be energized, usually having two electrodes; the cell electro-rotation instrument is used together with the electric shock tube, and when the cell electro-rotation instrument is in use, the liquid sample is located in the electric shock tube
  • the electric shock tube is placed in the socket of the fixing base, and the electrode terminal 1 and the electrode terminal 2 respectively abut against the two electrodes of the electric shock tube, the first electrode and the second electrode, and the electrode terminal and the electrode terminal can be made under the action of the spring Second pressing on the two electrodes of the electric shock tube to form an electrical connection
  • the power module can be activated to energize the electrode terminal 1 and the electrode terminal to form an electric field in the electric shock tube, thereby injecting extracellular substances into the cell.
  • the electric shock tube is vertically disposed, and the two electrodes of the electric shock tube are disposed above and below; the electrode terminal 1 and the electrode terminal 2 are
  • a display screen is disposed on the casing, and a sampler for sampling current or voltage values of the two is connected to the electrode terminal 1 and the electrode terminal 2, and the micro control unit is The display screen and the sampler are coupled, and the electrical signal collected by the sampler can be transmitted to the micro control unit and displayed as a waveform on the display screen.
  • the power module can generate electrical pulses required for cell electroporation.
  • the display screen is set on the cabinet to display the instrument usage status and experimental operation data.
  • the micro control unit can control the power module and the display.
  • the micro control unit includes a programmable microcontroller and Other microprocessors, etc., the sampler can collect electrical signals during cell electroporation, including voltage or current signals.
  • the sampler includes electronic components such as resistors.
  • the electrical signals are processed by the micro control unit and displayed on the display in the form of waveforms.
  • the micro control unit can also calculate the current, voltage, total energy and other parameters of the electrical pulse flowing through the cell sample and display these parameters directly on the display.
  • the temperature change of the solution during electroporation can also be reflected in the waveform diagram.
  • the display screen can receive the data signal transmitted by the micro control unit and display an operation interface and experimental state information, the display screen is a touch screen capable of inputting a user instruction, and the micro control unit The user command transmitted by the display screen can be received and the working state of the power module can be controlled according to the instruction.
  • the touch screen operation is convenient and sensitive.
  • the fixing base includes a seat body and a clamping cylinder, the insertion hole is opened in the clamping cylinder, a socket is defined in the seat body, and the clamping cylinder is inserted In the slot and the two are detachably interconnected.
  • Clamping cylinder and slot in the housing The detachable connection can replace the clamping cylinder of the corresponding jack aperture according to the diameter of different electric shock tubes, which can improve the versatility of the cell electrical converter.
  • the holding cylinder is made of a material having a light transmittance of more than 50 scoops, and a bottom of the clamping cylinder abuts against a trough bottom of the slot, the top end of the clamping cylinder There is at least one grip provided.
  • the clamping cylinder is made of a light-transmitting material to facilitate observation of the state of the electric shock tube in the socket; after the handle is designed, it is convenient for the operator to assemble and disassemble the clamping cylinder.
  • a metal electrode extension can be installed under the clamping cylinder to accommodate a shorter electric shock tube.
  • the metal extension is in contact with the electrode terminal, and the shorter electric shock tube is inserted into the clamping cylinder and the first electrode is
  • the electrode terminals are electrically connected by a metal extension to supplement the shortage of the height of the electric shock tube.
  • a sensor for detecting a displacement change of the electric shock tube is disposed in the seat body, and the sensor and the micro control unit are coupled to transmit the access state of the electric shock tube to the micro control unit, and the access state of the electric shock tube Can be displayed on the display.
  • an indicator light is further disposed in the casing, the indicator light is connected to the power module, and the signal of the sensor can be transmitted to the micro control unit for controlling the indicator light, It is convenient for the user to understand the access status of the electric shock tube. Generally, it can be set to be off when the electric shock tube is not connected. When the electric shock tube is connected, the indicator light is on, which is convenient for the user to observe. In addition to indicating the connection state of the electric shock tube, the indicator light can also have an illumination function, and a high-intensity indicator light such as an L E D lamp can be used to illuminate the electric shock tube.
  • the senor may be a mechanical trigger switch, and the pressure bar trigger switch that is linked when the electrode terminal is displaced; or may be a photoelectric switch, which affects the optical path when the electrode terminal is displaced to trigger the photoelectric
  • the switch can also be a Hall switch or the like, and can be triggered when the electric shock tube or the electrode terminal is displaced to control whether the indicator light is illuminated or not.
  • the side wall of the fixing seat is provided with a horizontally penetrating illumination hole
  • the indicator light is installed in the illumination hole
  • the illumination hole leads to the clamping tube
  • the clamping tube is made of a transparent material.
  • the indicator light on the fixed seat can pass through the clamping cylinder
  • the side illuminates the electric shock tube for a clearer visual effect.
  • the number of indicators can be single, two or more, illuminating the electric shock tube from the side of the mount.
  • the indicator light can also be mounted near the jack of the clamping cylinder, and the light illuminates the electric shock tube.
  • the casing is provided with a cover plate for covering the outer end of the jack, and the cover plate may be made of a material having a light transmittance of more than 50 scoops, and the electrode terminal is fixed to the jack.
  • the inner end of the cover is fixed on the cover, the inner end of the cover is hinged to the casing, the outer end of the cover is provided with a buckle, and the casing is further provided with a The hook of the snap-fit card is connected.
  • One end of the cover is hinged to the casing, and the other end is snapped to the casing for convenient closing and opening. After the cover is closed, the card can be connected to ensure good contact between the electrode terminal 2 and the electric shock tube.
  • an inner cavity is further defined in the body of the socket, and a through hole is defined in a groove bottom of the slot, and two ends of the through hole respectively form a cavity with the inner cavity and the clamping cylinder
  • a spring seat and the spring are disposed, an inner end of the spring is fixed on the spring seat, and an outer end of the spring is connected to an inner end of the electrode terminal, the electrode The outer end of the terminal one can pass through the through hole and be inserted into the insertion hole of the clamping cylinder.
  • the spring seat and the spring are designed, when the cover covers the opening of the jack, the first electrode and the second electrode of the two electrodes of the electric shock tube are electrically connected to the electrode terminal 1 and the electrode terminal 2, respectively, when the cover plate presses the electric shock tube
  • the spring is compressed to create a greater spring force such that the electrode terminals form a stable electrical connection against the electric shock tube.
  • the spring force generated by the spring is transmitted to both ends of the electric shock tube, so that the electric shock tube can seal the inner cell sample more reliably, and the electrochemical bubbles generated by the electrode attachment during the cell electric shock are compressed to some extent.
  • the bubble Since the bubble is substantially non-conductive, the current can only pass through the bubble gap, resulting in a large bubble gap current and easy cell death, while the current before and after the bubble (in the direction of the total current) is small and the cell is not easily electroporated.
  • the pressure generated by the spring can be conducted to the liquid sample during electroporation to counter the pressure of the bubble, the influence of the bubble on the current distribution is reduced, and the electroporation effect is improved.
  • only ordinary springs that provide electrical connection contact pressure require less pressure, especially when the voltage is higher at several hundred to several thousand volts, and relatively small pressures such as A pressure of less than 1 N (bovine) is sufficient to provide a good electrical connection.
  • the spring of the cell electrical instrument of the invention not only has the function of providing the pressure of the electrical contact, but also needs to have the function of generating a large pressure against the pressure of the electrolytic bubble, and the spring can generally generate a pressure of more than 1 N, or more than 2N. , or 4N or higher or even higher.
  • the electric shock tube can be ejected from the socket of the clamping tube under the action of the spring, which is convenient for picking up.
  • the casing is provided with a socket, and the socket is inserted with a slidable tray, and the fixing base is mounted on the tray, and the tray can be
  • the electric shock tube inside the mount pushes or pulls out the socket.
  • the electric shock tube can be placed into the jack or taken out from the jack.
  • the first electrode and the second electrode of the electric shock tube can be separated from the electrode terminal 1 and the electrode terminal 2 at least one, and when When the tray is pushed in, the electric shock tube establishes stable contact with the electrode terminal 1 and the electrode terminal 2 so that electroporation can be performed.
  • the drawer tray is used to push or pull the electric shock tube containing the liquid sample from the side to complete the electroporation process, which is convenient.
  • Different clamping cylinders can also be used in the fixing seat to accommodate different sizes of electric shock tubes.
  • the sensor that detects the connection status of the electric shock tube and the status or illumination indicator can also be applied.
  • the spring in the present solution can be similarly disposed in the fixing seat. When the electric shock tube containing the liquid sample is pushed into the casing from the side through the tray, the spring compression can press the electrode terminal 1 and the electrode terminal 2 to the two electrodes of the electric shock tube. on.
  • the spring in the solution may also be one or two springs or spring pieces disposed in the casing. When the tray pushes the electric shock tube into the casing, the spring is compressed so that the electrode terminal 1 and the electrode terminal 2 are pressed against the two sides of the electric shock tube. On the electrode.
  • the present invention has the following advantages:
  • the display screen set on the casing is used to display the instrument usage status and experimental operation data.
  • the actual electrical signal during electroporation is processed by the micro control unit and displayed on the display in the form of a waveform diagram. Easy to observe.
  • the clamping cylinder and the slot in the seat body are detachably connected, and the clamping cylinder of the corresponding jack aperture size can be replaced according to the diameter of the different electric shock tube, thereby improving the versatility of the cell electrical converter.
  • This indicator is equipped with an indicator light to indicate the connection status of the electric shock tube. At the same time, it also has an illumination effect on the electric shock tube, which is helpful for the experimenter to observe and control the experiment.
  • the spring of the electric instrument can press the two electrodes of the electric shock tube to enhance the sealing of the electric shock tube, and compress the bubbles generated by the electrochemical reaction to some extent to improve the electroporation.
  • 1 is a schematic view showing the internal structure of the electric converter in the first embodiment.
  • Figure 2 is a partial enlarged view of Figure 1.
  • FIG. 3 is a schematic view showing the internal three-dimensional structure of the electric converter in the first embodiment.
  • Figure 4 is a flow chart of the control circuit of the present electric instrument.
  • Figure 5 is a schematic perspective view of the electrical converter.
  • Figure 6 is a schematic view showing the structure of the electric converter in the second embodiment.
  • Embodiment 1 The electric shock tube 20 is a tubular device capable of containing a liquid sample and capable of being energized, usually having two electrodes; the cell electrical transducer is used in conjunction with the electric shock tube 20.
  • the cell electrical instrument comprises a casing 1, a power module 6 and a micro control unit 7, the micro control unit 7 and the power module 6 are electrically connected, and the cell electrical converter is provided with a fixing base 2, the machine An electrode terminal 4 and an electrode terminal 2 5 electrically connected to the power module 6 are also fixed in the shell 1, and the electrode terminal 4 and the electrode terminal 2 are respectively used for supplying power to the two electrodes of the electric shock tube 20.
  • the fixing base 2 includes a seat body 21 and a clamping cylinder 22, and a clamping hole 221 for fixing the electric shock tube 20 is defined in the clamping cylinder 22.
  • a socket 21 1 is defined in the base body 21, and the clamping cylinder 22 is inserted into the insertion hole
  • the clamping cylinder 22 is made of a material having a light transmittance of more than 50 scoops, and the bottom of the clamping cylinder 22 abuts against the trough bottom of the slot 21 1 to clamp the cylinder 22
  • the top end is provided with at least one handle 222, and a cavity 23 is defined in the seat body 21.
  • the bottom of the slot 21 1 is provided with a through hole 24, and the two ends of the through hole 24 are respectively connected with the inner cavity 23 and the clamping cylinder 22.
  • the jack 221 is in communication, and the inner chamber 23 is provided with a spring seat 16 and a spring 3, and the inner end of the spring 3 It is fixed to the spring seat 16 , and the outer end of the spring 3 is connected to the inner end of the electrode terminal 4 , and the outer end of the electrode terminal 4 can pass through the through hole 24 and is inserted into the insertion hole 221 of the clamping cylinder 22 .
  • the micro control unit 7 is capable of receiving user commands and performing control on the power module 6.
  • a display screen 8 is disposed on the casing 1, and a sampler 9 for sampling current or voltage values for the two is connected to the electrode terminal 4 and the electrode terminal 2, and the micro control unit 7 and the display screen are connected. 8 and the sampler 9 are connected, the electrical signal collected by the sampler 9 can be transmitted to the micro control unit 7 and displayed on the display screen 8 in the form of a waveform curve, and the display screen 8 can receive the data signal transmitted by the micro control unit 7.
  • the display screen 8 is a touch screen capable of inputting a user instruction
  • the micro control unit 7 is capable of receiving a user instruction transmitted from the display screen 8 and controlling the working state of the power module 6 according to the instruction; the power module 6
  • the electric pulse required for cell electroporation can be generated, and the display screen 8 provided on the casing 1 is used to display the instrument use state and experimental operation data, and the micro control unit 7 can control the power source.
  • the micro control unit 7 includes a programmable single chip microcomputer and other microprocessors, etc.
  • the sampler 9 can collect electrical signals during cell electroporation, including voltage or current signals
  • the sampler 9 includes resistors and the like.
  • the component, the electrical signal is processed by the micro control unit 7, and displayed on the display screen 8 in the form of a waveform diagram for convenient observation.
  • the current, voltage, total energy and the like of the pulse electrical signal can also be displayed on the display screen 8 at the same time.
  • a sensor 1 2 for detecting a change in displacement of the electric shock tube 20 is disposed in the base 21, and the sensor 12 and the micro control unit 7 are coupled, and an indicator light 1 is also disposed in the casing 1. 1 .
  • the side wall of the seat body 21 is provided with an illumination hole 10 extending through the horizontal direction.
  • the indicator light 1 1 is located in the illumination hole 10 , and the indicator light 1 1 is connected to the power module 6 , and the signal of the sensor 12 can be transmitted to the micro control unit 7 .
  • the number of the indicator lights 1 1 is two and respectively located on both sides of the fixed seat 2; the two sides of the fixed seat 2 are provided with a horizontally penetrating illumination hole 1 0 , and the indicator light 1 1 is installed in the illumination In the hole 10, the illumination hole 10 leads to the clamping cylinder 22, and the clamping cylinder 22 is made of a transparent material.
  • the indicator light 1 on the fixing base 2 can illuminate the electric shock tube 20 from the side through the clamping cylinder 22 to form a comparison. Clear visual effect, the indicator light 1 1 uses a higher brightness indicator 1 1 such as an LED light to illuminate the electric shock tube 20.
  • the sensor 12 may be a mechanical trigger switch.
  • the pressure bar trigger switch When the electrode terminal 4 is displaced, the pressure bar trigger switch is connected thereto; or may be a photoelectric switch, and when the electrode terminal 4 is displaced, the light path is affected to trigger the photoelectric switch; It may be a Hall switch or the like, and when the electric shock tube 20 or the electrode terminal 4 is displaced, it can be triggered to control whether the indicator light 1 1 is illuminated or not.
  • the casing 1 is provided with a cover plate 13 for covering the outer end of the socket 221, and the cover plate 13 is made of a material having a light transmittance of more than 50 scoops, and the electrode terminal 4 is fixed to the jack 221
  • the inner end, the electrode terminal 2 is fixed on the cover 13
  • the inner end of the cover 13 is hinged to the casing 1
  • the outer end of the cover 13 is provided with a buckle 14
  • the casing 1 is also provided with a buckle 14-phase snap hook 1 5 .
  • the cover 13 is hinged to the casing 1 and the other end is engaged with the casing 1 to facilitate closing and opening.
  • the card is caught to ensure good contact between the electrode terminal 2 and the electric shock tube 20.
  • the liquid sample is located in the electric shock tube 20, and the electric shock tube 20 is placed in the insertion hole 221 of the fixing base 2, when the cover 13 covers the opening of the insertion hole 221, the electrode terminal 4 and the electrode terminal 2 5 abutting against the two electrodes of the electric shock tube 20 respectively, under the action of the spring 3, the electrode terminal 4 and the electrode terminal 2 can be pressed against the two electrodes of the electric shock tube 20, thereby forming an electrical connection, and the cover 13 is pressed.
  • the spring 3 When the electric shock tube 20 is held, the spring 3 is compressed to generate a large elastic force so that the electrode terminal 4 is tightly pressed against the electric shock tube 20 to form a stable electrical connection.
  • the cover plate 13 When the cover plate 13 is opened after the cell electric shock is completed, the electric shock tube 20 can be ejected from the insertion hole 221 of the clamping cylinder 22 by the spring 3 to facilitate picking; the user command is received by the micro control unit 7 and the power module 6 is implemented.
  • the power module 6 can be activated to energize the electrode terminal 4 and the electrode terminal 2, so that an electric field is formed in the electric shock tube 20, thereby injecting extracellular substances into the cells; the clamping tube 22 and the slot 21 in the housing 21 1 detachable connection, according to the diameter of the different electric shock tube 20, the corresponding clamping hole 22 of the aperture 221 of the corresponding socket 221 is replaced.
  • the handle 222 is designed, the operator can easily disassemble the clamping cylinder 22 and improve the electric rotation of the cell.
  • the holding cylinder 22 is made of a light transmissive material to facilitate observation of the state of the electric shock tube 20 in its insertion hole 221.
  • Embodiment 2 is a diagrammatic representation of Embodiment 1:
  • the embodiment is substantially the same as the first embodiment, except that the casing 1 of the electrical converter is provided with a socket 17, and the socket 17 is slidably inserted into the socket 17, and the fixing base 2 is disposed.
  • the tray 18 can push or pull the electric shock tube 20 in the insertion hole 221 of the fixing base 2 into or out of the socket 17, and push or pull the electric shock tube 20 containing the liquid sample from the side by the drawer type tray 18. It is convenient to make the socket 1 7 to complete the electroporation process.
  • the electric shock tube 20 When the tray 18 is pulled out, the electric shock tube 20 can be placed or removed from the insertion hole 221, and the electric shock tube 20 can be at least one out of contact with the electrode terminal 4 and the electrode terminal 2, and when the tray 18 is At the time of pushing in, the two electrodes of the electric shock tube 20 are in contact with the electrode terminal 4 and the electrode terminal 2 to allow electroporation to proceed.
  • the electrode terminal 4 or the electrode terminal 2 itself has a certain elasticity or uses a spring to make it elastic, which facilitates a good electrical connection with the electric shock tube 20. Touch. Different clamping cylinders 22 can also be used in the mount 2 to accommodate different sizes of the electric shock tube 20.
  • the sensor 12 and the illumination indicator 1 1 for detecting the connection state of the electric shock tube 20 may also be mounted near the base 2.

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Abstract

一种细胞电转仪，属于生物医药仪器设备技术领域。它解决了在对细胞样品电转处理时不方便等技术问题。本细胞电转仪包括机壳（1）、电源模块（6）和微控制单元（7），细胞电转仪内设置有固定座（2），固定座（2）上开设有用于固定电击管（20）的插孔（221），机壳（1）内还设有与电源模块（6）相电连接的电极端子一（4）及电极端子二（5），电极端子一（4）及电极端子二（5）分别用于抵靠在电击管（20）的两个电极上实现供电，机壳（1）内还设有用于将电极端子一（4）和/或电极端子二（5）压紧在电击管（20）的两电极上的弹簧（3），微控制单元（7）能够接收用户指令并对电源模块（6）实行控制。通过机壳（1）上设置的显示屏（8）用来显示仪器使用状态和实验操作数据，便于观察。

Description

一种细胞电转仪 技术领域
本发明属于生物医药仪器设备技术领域， 涉及一种细胞电转 仪。 背景技术
细胞电穿孔（亦称细胞电转染或细胞电转）是利用电脉冲向细 胞内引入无法穿透细胞膜的大分子的技术。 电穿孔是一种广泛采 用并得到强烈推荐的用于细胞试验和基因疗法的方法。 在施加强 电场时 ， 细胞膜暂时变成多孔性质并且可以透过外界材料， 诸如 大分子。 细胞膜电穿透作用取决于电场的各种参数 ， 诸如脉冲类 型、 脉冲电压、 脉冲持续时间、 脉冲数量以及其他实验条件。 目 前用于细胞电穿孔的设备主要有细胞电转仪、 电极杯等。
我国专利 （公开号 ： CN1965079B , 公告日 ： 2007- 05- 16 )公 开了一种具有长中空件的电穿孔装置 ， 该电穿孔装置包括长的中 空件 ， 以便在电穿孔过程中提供均匀的电场 ， 其中特别是 ， 执行 电穿孔是通过在中空件充满包括细胞和将要注入细胞的物质的液 体样本之后 ， 由一对电极从长的中空件两个末端施加电脉冲。
本申请人曾申报过一篇名为 电穿孔的方法和装置"（ ^THODS AND DEVI CES FOR ELECTROPORATI ON ; US 2013/ 005271 1 A1 )的美 国专利 ， 该专利中记载了一种样品容器 ， 在这里以电击管代称 ， 电击管亦即相当于前述美国专利申请中的样品容器 ， 其作用是在 管内加入含有细胞和将要注入细胞的物质的液体样本 ，在电击管 的上、 下两端分别设置上电极和下电极 ， 通过细胞电转仪与上电 极和下电极相连接 ， 从而使电击管内形成电场 ， 进而使细胞外的 物质注入细胞内。 而在将液体样本填注入电击管内后 ，在开始电 击之前 ， 电击管内需要防止有空气残留形成气泡影响电流分布。 在该专利中 ， 申请人于电击管的管壁上设计有一与电击管腔体相 连通的环形凹槽 ， 实验者可持续向电击管腔体中注入液体样本直 至液体表面凸出电击管腔体后 ， 再将上电极盖下与液体凸面接触 直至压到电击管腔体的上沿 ， 形成对腔体里面液体的密封 ， 而少 量溢出的液体流到环形凹槽中。 这种设计一般可以防止电击管内 有残留空气影响实验。
本申请人在不断实践中结合上述现有技术 ， 设计出一款新的 细胞电转仪器 ， 以提高实验者操作的可靠性和便捷性。 发明内容
本发明提供了一种细胞电转仪 ， 本发明所要解决的技术问题 是 ： 如何与电击管配合实现对细胞样品的稳定电转处理。
本发明的目的可通过下列技术方案来实现 ：
一种细胞电转仪 ， 所述细胞电转仪包括机壳、 电源模块和微 控制单元 ， 所述微控制单元和电源模块相电联接 ， 其特征在于 ， 所述细胞电转仪内设置有固定座 ， 所述固定座上开设有用于固定 电击管的插孔 ， 所述机壳内还设有与所述电源模块相电连接的电 极端子一及电极端子二 ， 所述电极端子一及电极端子二分别用于 抵靠在电击管的两个电极上实现供电 ， 所述机壳内还设有用于将 所述电极端子一和 /或电极端子二压紧在电击管的两电极上的弹 簧 ， 所述微控制单元能够接收用户指令并对电源模块实行控制。
其原理如下 ： 电击管是一种内部能够盛装液体样本且可以通 电的管状器件 ， 通常具有两个电极； 本细胞电转仪与电击管配合 使用 ， 本细胞电转仪在使用时 ， 液体样本位于电击管内 ， 电击管 置于固定座的插孔中 ， 电极端子一和电极端子二分别与电击管的 两个电极第一电极和第二电极相抵靠 ，在弹簧的作用下能够使电 极端子一和电极端子二压紧在电击管的两电极上 ， 从而形成电联 接 ， 通过微控制单元接收用户指令并对电源模块实行控制 ， 能够 启动电源模块与电极端子一和电极端子二通电 ， 使电击管内形成 电场 ，进而使细胞外的物质注入细胞内。 作为优选 ，电击管竖直 设置 ， 且电击管的两电极上、 下设置； 电极端子一和电极端子二 相应的设置在电击管的上、 下方。
在上述的细胞电转仪中 ， 所述机壳上设置有一显示屏 ， 所述 电极端子一和电极端子二上联接有用于对两者进行电流或电压值 采样的采样器 ， 所述微控制单元与显示屏及采样器相联接 ， 所述 采样器采集到的电信号能够传到微控制单元并且在显示屏上以波 形曲线的形式显示。电源模块可以产生细胞电穿孔需要的电脉冲 ， 机壳上设置的显示屏用来显示仪器使用状态和实验操作数据 ， 微 控制单元可以控制电源模块和显示屏 ， 微控制单元包括可编程的 单片机和其他微处理器等 ， 采样器可以采集细胞电穿孔过程中的 电信号 ， 包括电压或者电流信号 ， 采样器包括电阻等电子元件 ， 电信号经由微控制单元处理 ， 以波形图的形式显示在显示屏上 ， 便于观察 ，微控制单元也可以计算流过细胞样品的电脉冲的电流、 电压、 总能量等参数并且将这些参数直接在显示屏上显示。 通过 观察波形图 ， 可以检查实验的质量 ，特别是波形图出现异常时 ， 可以及时了解实验中出现的问题。电穿孔过程中的溶液温度变化 ， 在波形图上也可以得到一定的反映。
在上述的细胞电转仪中 ， 所述显示屏能够接收所述微控制单 元传送过来的数据信号并显示操作界面和实验状态信息 ， 所述显 示屏为能够输入用户指令的触摸屏 ， 所述微控制单元能够接收所 述显示屏传送过来的用户指令并根据该指令来控制所述电源模块 的工作状态。 触摸屏操作使用方便且灵敏。
在上述的细胞电转仪中 ， 所述固定座包括座体和夹持筒 ， 所 述夹持筒内开设有所述的插孔 ， 所述座体内开设有一插槽 ， 所述 夹持筒插设于插槽中且两者可拆卸互连。 夹持筒与座体内的插槽 可拆卸连接 ， 则可根据不同电击管的直径大小 ， 更换相应插孔孔 径大小的夹持筒 ， 可提高本细胞电转仪的通用性。
在上述的细胞电转仪中 ， 所述夹持筒由透光率大于 50 勺材 料制成 ， 所述夹持筒的底部与所述插槽的槽底相贴靠 ， 所述夹持 筒的顶端设置有至少一个握柄。 夹持筒由透光材料制成便于观察 其插孔内的电击管状态；设计有握柄后 ，方便操作者装拆夹持筒。
作为其他一般情况，还可以在夹持筒下方安装金属电极延长 件来适应较短的电击管 ， 该金属延长件下方与电极端子一接触 ， 较短的电击管插入夹持筒后第一电极与电极端子一通过金属延长 件实现电连接 ， 这样来补充电击管高度的不足。
在上述的细胞电转仪中 ， 所述座体内设置有用于检测电击管 位移变化的传感器 ， 传感器和微控制单元相联接 ， 使电击管的接 入状态传到微控制单元 ，电击管的接入状态可以在显示屏上显示。
在上述的细胞电转仪中 ， 所述机壳内还设置有指示灯 ， 所述 指示灯与所述电源模块连接 ， 所述传感器的信号能够传到所述微 控制单元用来控制指示灯 ， 更加方便使用者了解电击管的接入状 态。 一般可以设置为当电击管未接入时 ， 指示灯不亮 ， 当电击管 接入时 ，指示灯亮起 ， 方便使用者观察。 所述指示灯除了能够指 示电击管连接状态 ， 同时也可以具有照明作用 ， 可以采用较高亮 度的指示灯比如 L E D灯等来照亮电击管。
在上述的细胞电转仪中 ，传感器可以是机械性触发开关，当电 极端子一发生位移时与其联动的压杆触发开关； 也可以是光电开 关 ， 当电极端子一发生位移时对光路产生影响触发光电开关； 传 感器还可以是霍尔开关等 ， 当电击管或者电极端子一发生位移时 就能触发从而控制指示灯发光与否。
在上述的细胞电转仪中 ， 所述固定座的侧壁开设有横向贯穿 的照明孔 ， 所述指示灯安装在照明孔内 ， 照明孔通向夹持筒 ， 夹 持筒由透明材料制作 ， 所述固定座上的指示灯可以透过夹持筒从 侧面照亮电击管 ， 形成比较清晰的视觉效果。 指示灯的数量可以 为单个 ， 两个或者多个 ， 从所述固定座的侧面照亮电击管。 作为 其他代替情况，指示灯还可以安装在夹持筒的插孔附近 ，灯光就 近照亮电击管。
在上述的细胞电转仪中 ， 所述机壳上设置有用于遮盖插孔外 端的盖板 ， 所述盖板可以由透光率大于 50 勺材料制成 ， 所述电 极端子一固定在插孔的内端 ， 所述电极端子二固定在盖板上 ， 所 述盖板的内端铰接于机壳上 ， 所述盖板的外端设置有卡扣 ， 所述 机壳上还设置有能够与所述卡扣相卡接的卡钩。 盖板一端铰接于 机壳上 ， 另一端与机壳相卡接 ， 方便关闭与打开 ， 盖板关闭后卡 接住可以保证电极端子二与电击管的良好接触。
在上述的细胞电转仪中 ， 所述座体内还开设有一内腔 ， 所述 插槽的槽底开设有通孔 ， 所述通孔的两端分别与所述内腔和夹持 筒的插孔相连通 ， 所述内腔中设置有弹簧座和所述弹簧 ， 所述弹 簧的内端固定于弹簧座上 ， 所述弹簧的外端与所述电极端子一的 内端相连接 ， 所述电极端子一的外端能够穿过所述通孔并插设于 夹持筒的插孔中。 设计有弹簧座和弹簧后 ， 当盖板遮闭插孔开口 时 ， 电击管的两个电极第一电极和第二电极分别与电极端子一和 电极端子二电连接 ， 盖板压住电击管时弹簧被压缩产生较大的弹 力使得电极端子一紧紧抵靠电击管形成稳定的电联接。 弹簧产生 的弹力传到到电击管的两端 ， 使电击管可以更加可靠地密封里面 的细胞样品 ， 也使细胞电击过程中电极附件产生的电化学气泡受 到一定的压缩。 由于气泡基本不导电 ，电流只能从气泡间隙通过 ， 导致气泡间隙电流大而细胞易死亡 ，而气泡前后（按总电流方向） 电流小而细胞不易电穿孔。 而弹簧产生的压力在电穿孔时可以传 导到液体样品上来对抗气泡的压力 ，气泡对电流分布的影响降低 ， 电穿孔效果提高。 一般仅提供电连接触点压力的普通弹簧需要的 压力不大，特别是电压较高在几百到上千伏时，比较小的压力比如 小于 1 N (牛）的压力足以提供良好的电连接。 本发明所述细胞电转 仪的弹簧不仅具有提供电触点压力的功能 ， 同时还需要具有产生 较大压力来对抗电解气泡压力的功能，弹簧压缩后一般能产生 1 N 以上的压力 ， 或者 2N以上 ， 或者 4N以上甚至更高。 细胞电击完 成后盖板打开时 ， 电击管可在弹簧的作用下从夹持筒的插孔中弹 出一部分 ， 方便拾取。
在上述的细胞电转仪中 ， 作为另一种方案 ， 所述机壳上开设 有一个插口 ， 所述插口处插接有可滑动的托盘 ， 所述固定座安装 在托盘上 ， 所述托盘能够将固定座里面的电击管推入或拉出所述 插口。 当所述托盘拉出时 ， 电击管可以放置到插孔或者从插孔取 出 ， 此时电击管的第一电极和第二电极可以与电极端子一和电极 端子二至少有一个脱离接触 ， 而当所述托盘推入时 ， 电击管与电 极端子一和电极端子二建立稳定接触使电穿孔可以进行。 本技术 方案中采用抽屉式托盘从侧面将盛有液体样本的电击管推进或拉 出插口 ， 使之完成电穿孔过程 ， 比较方便。 所述固定座内也可以 采用不同的夹持筒来适应不同大小的电击管。 在这种方案里 ， 探 测电击管连接状态的传感器以及状态或者照明指示灯同样可以应 用。 本方案中的弹簧可以类似地设置在固定座里面 ， 通过托盘从 侧面将盛有液体样本的电击管推进机壳内时弹簧压缩能够使电极 端子一和电极端子二压紧在电击管的两电极上。 本方案中的弹簧 也可以是设置在机壳内的一个或者两个弹簧或者弹簧片 ， 当托盘 将电击管推进机壳内时弹簧压缩使电极端子一和电极端子二压紧 在电击管的两电极上。 与现有技术相比 ， 本发明具有以下优点 ：
1、本电转仪中通过机壳上设置的显示屏用来显示仪器使用状 态和实验操作数据 ， 电穿孔过程中实际的电信号经由微控制单元 处理 ， 以波形图的形式显示在显示屏上 ，便于观察。 2、 本电转仪中夹持筒与座体内的插槽可拆卸连接 ，则可根据 不同电击管的直径大小 ， 更换相应插孔孔径大小的夹持筒 ， 提高 了本细胞电转仪的通用性。
3、 本电转仪中设置有指示灯 ，能够指示电击管连接状态 ，同 时也对电击管具有照明作用 ， 有助于实验者观察与控制实验的进 行。
4、 本电转仪的弹簧可以压紧电击管的两个电极 ，增强电击管 的密封性 ， 在一定程度上压缩电化学反应产生的气泡改善电穿孔
^5 o 附图说明
图 1是实施例一中本电转仪内部结构示意图。
图 2是图 1 中的局部放大图。
图 3是实施例一中本电转仪内部立体结构示意图。
图 4是本电转仪电路控制流程图。
图 5是本电转仪的立体结构示意图。
图 6是实施例二中本电转仪的结构示意图。
图中 ， 1、 机壳； 2、 固定座； 21、 座体； 21 1、 插槽； 22、 夹 持筒 ； 221、 插孔 ； 222、 握柄； 3、 弹簧； 4、 电极端子一 ； 5、 电 极端子二； 6、 电源模块； 7、 微控制单元； 8、 显示屏； 9、 采样 器 ； 10、 照明孔； 1 1、 指示灯 ； 12、 传感器； 13、 盖板； 14、 卡 扣 ； 1 5、 卡钩 ； 1 6、 弹簧座； 17、 插口 ； 18、 托盘； 20、 电击管； 23、 内腔； 24、 通孔。 具体实施方式
以下是本发明的具体实施例并结合附图 ， 对本发明的技术方 案作进一步的描述 ， 但本发明并不限于这些实施例。
实施例一 ： 电击管 20 是一种内部能够盛装液体样本且可以通电的管状 器件 ， 通常具有两个电极； 本细胞电转仪与电击管 20配合使用。
如图 1 至图 5所示 ， 本细胞电转仪包括机壳 1、 电源模块 6 和微控制单元 7 , 微控制单元 7和电源模块 6相电联接 ， 细胞电 转仪内设置有固定座 2 ,机壳 1 内还固设有与电源模块 6相电连 接的电极端子一 4及电极端子二 5 ,电极端子一 4及电极端子二 5 分别用于抵靠在电击管 20的两个电极上实现供电 ，固定座 2包括 座体 21和夹持筒 22 , 夹持筒 22内开设有用于固定电击管 20的 插孔 221 ,座体 21 内开设有一插槽 21 1 , 夹持筒 22插设于插槽 21 1 中且两者可拆卸互连 ， 夹持筒 22由透光率大于 50 勺材料制 成 ， 夹持筒 22的底部与插槽 21 1的槽底相贴靠 ， 夹持筒 22的顶 端设置有至少一个握柄 222 ,座体 21 内还开设有一内腔 23 ,插槽 21 1 的槽底开设有通孔 24 , 通孔 24的两端分别与内腔 23和夹持 筒 22的插孔 221相连通 ， 内腔 23中设置有弹簧座 16和弹簧 3 , 弹簧 3的内端固定于弹簧座 1 6上 ，弹簧 3的外端与电极端子一 4 的内端相连接 ，电极端子一 4的外端能够穿过通孔 24并插设于夹 持筒 22的插孔 221 中 丄述微控制单元 7能够接收用户指令并对 电源模块 6实行控制。
如图 5所示 ，机壳 1 上设置有一显示屏 8 , 电极端子一 4和 电极端子二 5上联接有用于对两者进行电流或电压值采样的采样 器 9 , 微控制单元 7与显示屏 8及采样器 9相联接 ， 采样器 9采 集到的电信号能够传到微控制单元 7并且在显示屏 8上以波形曲 线的形式显示 ， 显示屏 8能够接收微控制单元 7传送过来的数据 信号并显示操作界面和实验状态信息 ， 显示屏 8为能够输入用户 指令的触摸屏 ， 微控制单元 7能够接收显示屏 8传送过来的用户 指令并根据该指令来控制电源模块 6的工作状态； 电源模块 6可 以产生细胞电穿孔需要的电脉冲 ，机壳 1 上设置的显示屏 8用来 显示仪器使用状态和实验操作数据 ， 微控制单元 7可以控制电源 模块 6和显示屏 8 , 微控制单元 7包括可编程的单片机和其他微 处理器等 ， 采样器 9可以采集细胞电穿孔过程中的电信号 ， 包括 电压或者电流信号 ， 采样器 9包括电阻等电子元件 ， 电信号经由 微控制单元 7处理 ，以波形图的形式显示在显示屏 8上便于观察 ， 脉冲电信号的电流、 电压、 总能量等信息也可以同时显示在显示 屏 8上。
如图 2、 图 3和图 4所示 ，座体 21 内设置有用于检测电击管 20位移变化的传感器 1 2 , 传感器 12和微控制单元 7相联接 ， 机 壳 1 内还设置有指示灯 1 1 ,座体 21 的侧壁开设有沿横向贯穿的 照明孔 10 ,指示灯 1 1位于照明孔 10内 ，指示灯 1 1与电源模块 6 连接 ， 传感器 12的信号能够传到微控制单元 7用来控制指示灯 1 1 ,指示灯 1 1的数量为两个且分别位于固定座 2的两侧 ；固定座 2的两侧均开设有横向贯穿的照明孔 1 0 ,指示灯 1 1安装在照明孔 10内 ，照明孔 1 0通向夹持筒 22 ,夹持筒 22由透明材料制作 ，固 定座 2上的指示灯 1 1可以透过夹持筒 22从侧面照亮电击管 20 , 形成比较清晰的视觉效果 ，指示灯 1 1 采用较高亮度的指示灯 1 1 比如 LED灯等来照亮电击管 20。 传感器 12可以是机械性触发开 关，当电极端子一 4发生位移时与其联动的压杆触发开关；也可以 是光电开关 ， 当电极端子一 4发生位移时对光路产生影响触发光 电开关； 传感器 12还可以是霍尔开关等 ， 当电击管 20或者电极 端子一 4发生位移时就能触发从而控制指示灯 1 1发光与否。
如图 1和图 5所示 ，机壳 1上设置有用于遮盖插孔 221外端 的盖板 13 ,盖板 13由透光率大于 50 勺材料制成 ，电极端子一 4 固定在插孔 221 的内端 ， 电极端子二 5固定在盖板 13上 ， 盖板 13的内端铰接于机壳 1上 ， 盖板 13的外端设置有卡扣 14 ,机壳 1 上还设置有能够与卡扣 14相卡接的卡钩 1 5。 盖板 13—端铰接 于机壳 1上 ，另一端与机壳 1相卡接 ，方便关闭与打开 ，盖板 13 关闭后卡接住可以保证电极端子二 5与电击管 20的良好接触。 本细胞电转仪在使用时 ，液体样本位于电击管 20内 ，电击管 20置于固定座 2的插孔 221 中 ，当盖板 13遮闭插孔 221开口时 ， 电极端子一 4和电极端子二 5分别与电击管 20的两个电极相抵 靠 ，在弹簧 3的作用下能够使电极端子一 4和电极端子二 5压紧 在电击管 20的两电极上 ， 从而形成电联接 ， 盖板 13压住电击管 20时弹簧 3被压缩产生较大的弹力使得电极端子一 4紧紧抵靠电 击管 20形成稳定的电联接。 细胞电击完成后盖板 13打开时 ， 电 击管 20可在弹簧 3的作用下从夹持筒 22的插孔 221 中弹出一部 分 ， 方便拾取； 通过微控制单元 7接收用户指令并对电源模块 6 实行控制 ， 能够启动电源模块 6与电极端子一 4和电极端子二 5 通电 ，使电击管 20内形成电场 ，进而使细胞外的物质注入细胞内 ； 夹持筒 22与座体 21 内的插槽 21 1可拆卸连接 ， 则可根据不同电 击管 20的直径大小 ， 更换相应插孔 221 孔径大小的夹持筒 22 , 设计有握柄 222后 ，方便操作者装拆夹持筒 22 ,提高本细胞电转 仪的通用性。夹持筒 22由透光材料制成便于观察其插孔 221 内的 电击管 20状态。
实施例二 ：
如图 6所示 ，本实施例与实施例一大致相同 ，不同之处在于 ， 本电转仪的机壳 1 开设有插口 17 , 插口 17处可滑动的插接有托 盘 1 8 ,固定座 2设置在托盘 18上 ，托盘 18能够将固定座 2的插 孔 221 中的电击管 20推入或拉出插口 17 ,采用抽屉式托盘 1 8从 侧面将盛有液体样本的电击管 20推进或拉出插口 1 7 , 使之完成 电穿孔过程 ， 比较方便。 当托盘 18拉出时 ， 电击管 20可以放置 到插孔 221或者从插孔 221 中取出 ，此时电击管 20可以与电极端 子一 4和电极端子二 5至少有一个脱离接触 ，而当托盘 18推入时 ， 电击管 20的两电极与电极端子一 4和电极端子二 5接触使电穿孔 可以进行。 电极端子一 4或者电极端子二 5本身具有一定的弹性 或者使用弹簧使其具有弹性 ， 方便与电击管 20 形成良好的电接 触。固定座 2内也可以采用不同的夹持筒 22来适应不同大小的电 击管 20。探测电击管 20连接状态的传感器 12以及照明指示灯 1 1 也可以安装在座体 2附近。
文中所描述的具体实施例仅仅是对本发明精神作举例说明。 本发明所属技术领域的技术人员可以对所描述的具体实施例做各 种各样的修改或补充或采用类似的方式替代 ， 但并不会偏离本发 明的精神或者超越所附权利要求书所定义的范围。

Claims

权 利 要 求 书
1、 一种细胞电转仪 ，所述细胞电转仪包括机壳（ 1 λ 电源模 块（ 6 )和微控制单元（ 7 ) ,所述微控制单元（ 7 )和电源模块（ 6 ) 相电联接 ， 其特征在于 ， 所述细胞电转仪内设置有固定座（2), 所述固定座（2)上开设有用于固定电击管（ 20)的插孔（ 221 ), 所述机壳（ 1 )内还具有与所述电源模块（6)相电连接的电极端 子一（4)及电极端子二（5),所述电极端子一（4)及电极端子 二（ 5)分别用于抵靠在电击管（ 20)的两个电极上实现供电 ，所 述机壳（ 1 )内还设有用于将所述电极端子一（4)和 /或电极端子 二（ 5)压紧在电击管（ 20)的两电极上的弹簧（ 3) ,所述微控制 单元（7)能够接收用户指令并对电源模块（6)实行控制。
2、 根据权利要求 1所述的一种细胞电转仪 ，其特征在于 ，所 述机壳（ 1 )上设置有一显示屏（8),所述电极端子一（4)和电 极端子二（5)上联接有用于对两者进行电流或电压值采样的采样 器（ 9 ) ,所述微控制单元（ 7 )与显示屏（ 8 )及采样器（ 9 )相联 接 ， 所述采样器（9)采集到的电信号能够传到微控制单元（7) 并且在显示屏（8)上以波形曲线的形式显示。
3、 根据权利要求 2所述的一种细胞电转仪 ，其特征在于 ，所 述显示屏（8)能够接收所述微控制单元（7)传送过来的数据信 号并显示操作界面和实验状态信息 ，所述显示屏（ 8)为能够输入 用户指令的触摸屏 ，所述微控制单元（7)能够接收所述显示屏（8) 传送过来的用户指令并根据该指令来控制所述电源模块（6)的工 作状态。
4、 根据权利要求 1所述的一种细胞电转仪 ，其特征在于 ，所 述机壳（ 1 )上具有用于遮盖插孔（ 221 )外端的盖板（ 13) ,所述 电极端子一（ 4 )固定在插孔（ 221 )的内端 ，所述电极端子二（ 5 ) 固定在盖板（ 13)上 ，所述盖板（ 13)的内端铰接于机壳（ 1 )上 ， 所述盖板（ 13)的外端设置有卡扣 （ 14),所述机壳（ 1 )上还设 置有能够与所述卡扣 （ 14)相卡接的卡钩（ 15)。
5、 根据权利要求 4所述的一种细胞电转仪 ，其特征在于 ，所 述盖板（ 13) 由透光率大于 50 勺材料制成。
6、 根据权利要求 1所述的一种细胞电转仪 ，其特征在于 ，所 述机壳（ 1 )的侧面开设有插口 （ 17), 所述插口 （ 17)处可滑动 的插接有托盘（ 18) ,所述固定座（ 2 )设置在所述托盘（ 18 )上 ， 所述托盘（ 18)能够将固定座（2)推入或拉出所述插口 （ 17)。
7、根据权利要求 1或 2或 3或 4或 5或 6所述的一种细胞电 转仪 ，其特征在于 ，所述固定座（2)包括座体（21 )和夹持筒（22), 所述夹持筒（ 22) 内开设有所述的插孔（ 221 ), 所述座体（ 21 ) 内开设有一插槽（ 211 ),所述夹持筒（ 22)插设于插槽（ 211 )中 且两者可拆卸互连。
8、 根据权利要求 7所述的一种细胞电转仪 ，其特征在于 ，所 述夹持筒（22)由透光率大于 50 勺材料制成 ，所述夹持筒（22) 的底部与所述插槽（ 211 )的槽底相贴靠 ，所述夹持筒（ 22)的顶 端设置有至少一个握柄（ 222
9、 根据权利要求 7所述的一种细胞电转仪 ，其特征在于 ，所 述座体（21 ) 内设置有用于检测电击管（20)位移变化的传感器
( 12), 传感器（ 12)和微控制单元（7)相联接 ， 传感器探测到 的电击管（20)的连接状态能够显示在显示屏（8)上。
10、 根据权利要求 7所述的一种细胞电转仪 ， 其特征在于 ， 所述座体（21 ) 内设置有用于检测电击管（20)位移变化的传感 器（ 12),传感器（ 12)和微控制单元（ 7)相联接 ，所述机壳（ 1 ) 内还设置有指示灯（ 11 ) ,所述指示灯（ 11 )与所述电源模块（6) 连接 ，所述传感器（ 12 )的信号能够传到所述微控制单元（ 7 )用 来控制指示灯（ 11 )。
11、 根据权利要求 10所述的一种细胞电转仪 ， 其特征在于 ， 所述座体（ 21 )的侧壁开设有沿横向贯穿的照明孔（ 10) ,所述指 示灯（ 11 )位于所述照明孔（ 10) 内 ， 所述指示灯（ 11 )能够从 侧面为电击管（20)提供照明。
12、 根据权利要求 11所述的一种细胞电转仪 ， 其特征在于 ， 所述指示灯（ 11 )的数量为两个且分别位于所述固定座（ 2 )的两
13、 根据权利要求 7所述的一种细胞电转仪 ， 其特征在于 ， 所述座体（ 21 ) 内还开设有一内腔（23),所述插槽（ 211 )的槽 底开设有通孔（ 24) ,所述通孔的两端分别与所述内腔（ 23)和夹 持筒（ 22)的插孔（ 221 )相连通 ，所述内腔（ 23)中设置有弹簧 座（ 16),所述弹簧（3)的内端固定于弹簧座（ 16)上 ， 所述弹 簧（3)的外端与所述电极端子一（4)的内端相连接 ， 所述电极 端子一（4)的外端能够穿过所述通孔（24)并插设于夹持筒（22) 的插孔（ 221 ) 中。
14、 根据权利要求 7所述的一种细胞电转仪 ， 其特征在于 ， 所述座体（21 ) 内设置有用于检测电击管（20)位移变化的传感 器（ 12),传感器（ 12)和微控制单元（7)相联接 ， 传感器探测 到的电击管（20)的连接状态能够显示在显示屏（8)上。
15、 根据权利要求 7所述的一种细胞电转仪 ， 其特征在于 ， 所述座体（21 ) 内设置有用于检测电击管（20)位移变化的传感 器（ 12),传感器（ 12)和微控制单元（ 7)相联接 ，所述机壳（ 1 ) 内还设置有指示灯（ 11 ) ,所述指示灯（ 11 )与所述电源模块（6) 连接 ，所述传感器（ 12 )的信号能够传到所述微控制单元（ 7 )用 来控制指示灯（ 11 )。
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CN104357323B (zh) * 2014-12-02 2016-05-04 陈剑 一种细胞电转仪
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CN110437999A (zh) * 2019-07-15 2019-11-12 广州蛟龙细胞医药科技有限公司 一种电转仪
CN114196533A (zh) * 2021-11-01 2022-03-18 上海盟德生物科技有限公司 一种微通道细胞电转仪
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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102277297A (zh) * 2011-08-16 2011-12-14 中国科学院深圳先进技术研究院 植入式在体电转染装置
US20130052711A1 (en) * 2011-08-25 2013-02-28 Jian Chen Methods and devices for electroporation
CN202945245U (zh) * 2012-11-19 2013-05-22 中国科学技术大学 具有电流和阻抗检测功能的活体单细胞电穿孔装置
CN203382763U (zh) * 2013-07-05 2014-01-08 陶英亮 Cik细胞活化试剂盒
CN103865794A (zh) * 2014-03-18 2014-06-18 苏州文曲生物微系统有限公司 一种手持电穿孔装置
CN104357323A (zh) * 2014-12-02 2015-02-18 陈剑 一种细胞电转仪
CN204311065U (zh) * 2014-12-02 2015-05-06 陈剑 一种细胞电转仪

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA200610508B (en) * 2004-06-12 2009-01-28 Digital Bio Technology Co Ltd Electroporator having an elongated hollow member
CN101020892A (zh) * 2007-03-02 2007-08-22 清华大学 一种用于单细胞电穿孔的纳电极和系统
CN101857836B (zh) * 2009-11-10 2013-06-12 昆山文曲生物微系统有限公司 一种流式电穿孔装置及系统

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102277297A (zh) * 2011-08-16 2011-12-14 中国科学院深圳先进技术研究院 植入式在体电转染装置
US20130052711A1 (en) * 2011-08-25 2013-02-28 Jian Chen Methods and devices for electroporation
CN202945245U (zh) * 2012-11-19 2013-05-22 中国科学技术大学 具有电流和阻抗检测功能的活体单细胞电穿孔装置
CN203382763U (zh) * 2013-07-05 2014-01-08 陶英亮 Cik细胞活化试剂盒
CN103865794A (zh) * 2014-03-18 2014-06-18 苏州文曲生物微系统有限公司 一种手持电穿孔装置
CN104357323A (zh) * 2014-12-02 2015-02-18 陈剑 一种细胞电转仪
CN204311065U (zh) * 2014-12-02 2015-05-06 陈剑 一种细胞电转仪

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