WO2016015457A1

WO2016015457A1 - Trace liquid analysis detection head capable of eliminating stray light interference

Info

Publication number: WO2016015457A1
Application number: PCT/CN2015/070585
Authority: WO
Inventors: 朱哲华; 刘景会
Original assignee: 北京普析通用仪器有限责任公司
Priority date: 2014-07-28
Filing date: 2015-04-02
Publication date: 2016-02-04
Also published as: CN105319162B; CN105319162A

Abstract

A trace liquid analysis detection head capable of eliminating stray light interference. An angle of inclination is set for a bearing tray (3), so that on the premise that to-be-detected trace liquid (6) on a round transparent area of the bearing tray (3) does not fall off, stray light generated by the bearing tray (3) is deflected away from a light receiving area of a receiving optical fiber (5), and all light received by the receiving optical fiber (5) is light that passes through the to-be-detected trace liquid (6) then is reflected by a reflection plate (7), and the stray light reflected by the bearing tray (3) is prevented from entering an optical analysis system through the receiving optical fiber, and interference of the stray light to optical analysis on a trace liquid medium is eliminated, thereby greatly improving the analysis accuracy of a related optical analysis system to the trace liquid medium.

Description

Detection head for micro liquid analysis capable of eliminating stray light interference

Technical field

The invention relates to the field of optical analysis of trace liquids, in particular to a detection head for micro liquid analysis which can eliminate stray light interference.

Background technique

At present, an apparatus for optical analysis or absorption measurement of a small amount of a liquid medium, such as a drop of liquid medium, by means of light has been developed. The operation principle of such a device is that the liquid medium to be tested is placed on the carrier tray of the detection head, The detection head light is guided through the medium and reflected by the reflector, and then the detection head is conducted by the receiving optical fiber, and the detection head is connected with a certain optical analysis instrument to detect or analyze the luminosity, spectrophotometry, fluorescence or spectral fluorescence of the liquid medium to be detected. .

However, when the incident light passes through the lens carrier disk at an angle, due to the thickness of the carrier disk and the surface reflection, a certain amount of stray light is generated to be projected into the receiving area of the receiving fiber, and enters the analyzing instrument through the receiving fiber to interfere with the liquid to be detected. Data analysis of the media, which affects the accuracy of the analysis.

Summary of the invention

The object of the present invention is to provide a detection head for a small amount of liquid analysis capable of eliminating stray light interference, and to provide a certain tilt angle to the carrier tray. Under the premise that the trace liquid does not slip, the stray light generated by the reflection of the carrier disk is deflected out of the light receiving region of the receiving fiber, and the stray light generated by the carrier disk is prevented from entering the optical analysis system through the receiving fiber, thereby eliminating stray light to trace The interference caused by the analysis of the liquid medium improves the accuracy of the analysis of the trace liquid medium.

The technical solution of the present invention is:

A detection head for micro liquid analysis capable of eliminating stray light interference, comprising:

a housing, which is a vertically arranged hollow cylindrical structure;

a carrier disk covering the top opening of the casing and disposed at a certain inclination angle along a cross-sectional direction of the casing to form an inclined table top for closing the top end of the casing, the carrier plate being provided with Placing a transparent area of the trace liquid to be tested;

a concentrating device, which is a light-transmitting body capable of collecting light, the concentrating device is movably connected to the inside of the casing in an adjustable distance from the carrier tray and separates the interior of the casing into two independent space;

a reflective sheet disposed above the carrier tray.

Preferably, in the detecting head for analyzing a small amount of liquid which can eliminate stray light interference, a portion of the carrying surface of the carrying tray is coated with a hydrophobic layer to limit the amount of liquid to be detected to a circular transparent region to form a hemisphere. Shaped droplet

In addition, the transparent area of the carrier tray is set to a circle having a diameter of 1.2-2 mm, and the transparent area of the carrier disk has a thickness of 0.5-2 mm.

Preferably, in the detecting head for micro liquid analysis capable of eliminating stray light interference, the center of the circular transparent area of the carrying tray is combined with the center of the carrying tray and the center of the collecting device is disposed in the same vertical direction. On the central axis.

Preferably, in the detecting head for micro liquid analysis capable of eliminating stray light interference, the reflecting sheet is fixedly connected with a height adjustable bracket and the distance between the reflecting sheet and the carrying tray is related to the liquid to be detected. The height of the formed hemispherical droplets corresponds.

Preferably, in the detection head for micro liquid analysis capable of eliminating stray light interference, the distance between the concentrating device and the carrier tray can be from the circular shape of the carrier tray after the incident light passes through the concentrating device. The center of the transparent zone passes through and passes through the liquid to be detected and is focused on the reflective sheet.

Preferably, in the detecting head for micro liquid analysis capable of eliminating stray light interference, the bottom end of the housing is provided with a sealing plug for closing the bottom end of the housing and matching the inner diameter of the housing. The sealing plug is movably coupled to the housing by being embedded in the housing in a positionally adjustable manner.

Preferably, in the detecting head for micro liquid analysis capable of eliminating stray light interference, the illuminating device is movably connected to the housing in such a manner that the side wall of the sealing plug is along the housing The inner wall extends inwardly from the connecting device, and the concentrating device is fixedly connected with the connecting device and interlocked with the sealing plug to adjust the distance between the concentrating device and the carrying tray.

Preferably, in the detecting head for micro liquid analysis capable of eliminating stray light interference, the sealing plug is respectively provided with an incident optical fiber and a receiving optical fiber penetrating through the sealing plug, wherein the incident optical fiber and the receiving optical fiber are respectively disposed at a position of the sealing plug on both sides of the central axis, the receiving fiber a first end of the sealing plug is disposed as a light receiving area;

The micro liquid analysis detecting head for eliminating stray light interference is connected to the receiving optical fiber and the corresponding optical analysis device through the incident optical fiber.

Preferably, in the detection head for micro liquid analysis capable of eliminating stray light interference, the carrier disk is inclined at an angle of a cross section of the housing to deflect light reflected by the carrier disk out of the receiving fiber. The light receiving area and the micro liquid to be detected on the transparent area are not slipped, and the inclination angle of the carrier disk in the cross-sectional direction of the housing is less than or equal to 20 degrees.

Preferably, in the detection head for micro liquid analysis capable of eliminating stray light interference, when the thickness of the carrier disk is 0.9-1.1 mm, and the distance between the incident fiber and the receiving fiber is 0.3-0.5 mm, the bearing The optimal tilt angle of the disc is 2-3 degrees.

The invention has the following beneficial effects: a detecting head for analyzing a small amount of liquid which can eliminate stray light interference according to the present invention, and setting a certain inclination angle of the carrying tray to make a small amount to be detected on a circular transparent area of the carrying tray Under the premise that the liquid does not slip, the stray light generated by the reflection of the carrier disk is deflected out of the light receiving area of the receiving fiber, so that the light received by the receiving fiber is all reflected by the reflecting sheet after the trace liquid to be detected. The stray light of the carrier disk is prevented from entering the optical analysis system through the receiving fiber, thereby eliminating the interference caused by the stray light of the carrier disk to analyze the micro-liquid medium, and the accuracy of the analysis of the micro-liquid medium is greatly improved, compared with the general micro-analysis. The accuracy of the present invention is improved by more than 5% in the detection head for optical analysis of liquid.

DRAWINGS

1 is a schematic view showing the structure of a detection head for analyzing a trace liquid which can eliminate stray light interference according to the present invention;

2 is a schematic view showing an optical path of a detecting head for analyzing a small amount of liquid which can eliminate stray light interference according to the present invention;

Fig. 3 is a schematic view showing the optical path of a conventional detecting head device for performing light analysis on a trace amount of liquid.

detailed description

The present invention will be described in detail below with reference to the accompanying drawings in which: FIG.

As shown in FIG. 1 , a detection head for micro liquid analysis capable of eliminating stray light interference includes: a housing 1 which is a vertically arranged hollow cylindrical structure, and a bottom end is respectively provided with an incident optical fiber 4 and a receiving optical fiber. a carrying tray 3 covering the top end opening of the casing 1 and disposed at a certain inclination angle along a cross-sectional direction of the casing 1 to form an inclined table surface for closing the top end of the casing 1 The carrying tray 3 is provided with a transparent area for placing a small amount of liquid to be detected, the carrying tray 3 can be provided with a transparent quartz plate, and the trace liquid 6 to be detected is placed on the transparent area of the carrying tray 3; the collecting device 2 is a light that can be used A light-transmitting body that is gathered, for example, a convex lens or a sphere of quartz material or crystal material, the concentrating device 2 is movably connected to the inside of the casing 1 in an adjustable manner from the carrier tray 3 and the inside of the casing 1 Separated into two independent spaces; a reflective sheet 7 disposed on an upper portion of the carrier 3 of the optical path system and disposed at a distance from the carrier 3, the reflective sheet 3 and the reflective sheet The cross section of the housing 1 is parallel .

As shown in FIG. 2 or FIG. 3, when the incident light passes through the incident optical fiber 4 and passes through the concentrating device 2 at an angle, the focal length is adjusted by adjusting the distance between the concentrating device 2 and the carrier 3 to make the incident light pass through. The transparent region of the carrier 3 passes through the liquid to be detected 6 and is focused on the reflective sheet 7. The reflected light generated by the reflective sheet 7 passes through the transparent region of the carrier 3 in the opposite direction and the concentrating device 2 illuminates the receiving optical fiber 5. The light receiving area 9 completes the returning optical path of the light, and the incident optical fiber 4 and the receiving optical fiber 5 are connected by a monochromator and a corresponding optical detecting device, and the optical signal is transmitted into the detecting device to accurately analyze the medium of the micro liquid 6. However, in a general device, the carrier 3 is horizontally disposed. As shown in FIG. 3, when the incident light passes through the transparent region of the carrier 3, the transparent region of the carrier 3 has a certain thickness and the surface of the transparent region has a certain surface. The reflection function generates a certain amount of reflected light that does not pass through the trace liquid 6 to be detected, which is stray light 10, and such stray light 10 is directly reflected by the carrier disk 3 and reaches the receiving fiber 5 without passing through the trace liquid 6 to be detected. Zone 9 enters the analysis system and affects the accuracy of the analytical measurements. The invention adopts a certain inclination angle of the carrier tray 3, as shown in FIG. 2, the angle needs to ensure that the trace liquid 6 to be detected remains in the original position without being slipped, and does not affect the condition that the light passes through the transparent region normally. The generated stray light 10 is deflected out of the light receiving region 9 of the receiving fiber 5, thereby preventing the stray light 10 from entering the analysis system, eliminating the interference caused by the analysis and measurement of the stray light 10 to detect the trace liquid 6, and ensuring the accuracy of the analysis and measurement. Sex.

The detection head for micro liquid analysis capable of eliminating stray light interference, wherein the carrier tray 3 is transparent The bright region is set to a circular shape with a diameter of 1.2-2 mm. In the present invention, a circular transparent region having a diameter of 1.6 mm is disposed, and a portion other than the circular transparent region is coated with a hydrophobic substance to form a hydrophobic layer to limit the amount of the liquid to be detected 6 A hemispherical droplet is formed in a circular transparent region. Further, the carrier tray 3 is a transparent quartz plate, and a portion other than the circular transparent region is plated with an opaque film, so that the carrier disk 3 has a circular transparent region at the center and a ring-shaped portion having an opaque portion at the periphery. The transparent area of the carrier 3 and the carrier 3 has a thickness of 0.5-2 mm.

In addition, the inclination angle of the carrier 3 and the thickness of the carrier 3 and the distance between the incident optical fiber 4 and the receiving optical fiber 5 are directly related. The thickness of the carrier 3 in the present invention is 0.9-1.1 mm, which is the most Preferably, the distance between the incident optical fiber 4 and the receiving optical fiber 5 is 0.3-0.5 mm, and most preferably 0.4 mm, the optimal tilting angle of the carrying tray 3 is 2-3 degrees, and the angle generally does not exceed 20 degree. By performing simulation optical path analysis and prototype test verification, the stray light 10 generated by the reflection of the carrier disk 3 has a set energy value of 100% when the incident light energy reaches 100%, and the energy value of the reflected stray light 10 is less than 0.2. %, the present invention can eliminate interference of at least 99.8% of stray light 10, and can greatly improve the accuracy of measurement.

In the detection head for micro liquid analysis capable of eliminating stray light interference, the center of the circular transparent region of the carrier tray 3 is combined with the center of the carrier tray 3 and the center of the concentrating device 2 is disposed in the same vertical direction. On the central axis.

In the detection head for micro liquid analysis capable of eliminating stray light interference, the reflection sheet 7 is fixedly connected to a height adjustable bracket and the distance between the reflection sheet 7 and the carrier tray 2 and the trace liquid to be detected 6 The height of the formed hemispherical droplets corresponds. The reflective sheet 7 is a smooth planar lens that reflects light, and is mounted on the bottom of a certain reflecting device and corresponds to a circular transparent area of the carrying tray 3, and the reflecting surface faces the circular transparent area by adjusting the bracket. The height and position are such that the reflective sheet 7 just reaches the top of the hemispherical droplet formed by the trace liquid 6 to be detected, and does not destroy the structure of the hemispherical droplet, and reflects the light passing through the hemispherical droplet.

In the detection head for micro liquid analysis capable of eliminating stray light interference, in use, focus adjustment is performed by adjusting the distance between the concentrating device 2 and the carrier tray 3 as needed, the concentrating device 2 and the carrier tray The distance between the three passes through the concentrating device 2 after the incident light passes through the central position of the circular transparent region of the carrier disk 3 and passes through the liquid to be detected 6 to be focused on the reflection sheet 7.

In the detecting head for micro liquid analysis capable of eliminating stray light interference, the bottom end of the casing 1 is provided with a sealing plug 8 for closing the bottom end of the casing 1 and matching the inner diameter of the casing 1. The sealing plug 8 is inserted into the housing 1 in a positionally adjustable manner in operative connection with the housing 1 .

In the detecting head for analyzing a small amount of liquid which can eliminate stray light interference, the concentrating device 2 is fixedly connected to the casing 1 by fixed connection with the sealing plug 8, and the position of the sealing plug 8 can be conveniently adjusted. The position of the concentrating device 2 is adjusted. The illuminating device 2 is movably connected to the housing 1 in such a manner that the side wall of the sealing plug 8 extends inwardly along the inner wall of the housing 1 to connect the device, the concentrating device 2 and the The connecting device is fixedly coupled and interlocked with the sealing plug 8 to adjust the distance between the collecting device 2 and the carrying tray 3. The connecting device may be a plurality of connecting rods extending from the side wall of the sealing plug 8, or may be a hollow tube, and the concentrating device 2 may be fixed to the connecting rod or the hollow tube by bonding, thereby sealing the plug 8 follow.

In the detection head for micro liquid analysis capable of eliminating stray light interference, the sealing plug 8 is respectively provided with an incident optical fiber 4 and a receiving optical fiber 5 penetrating through the sealing plug 8, wherein the incident optical fiber 4 and the receiving optical fiber 5 are respectively Arranged at opposite positions on both sides of the central axis of the sealing plug 8, the first end of the receiving optical fiber 5 passing through the sealing plug 8 is provided as a light receiving region 9. The light is incident through the incident light 4, and after the reflection is completed, it reaches the light receiving region 9 of the receiving fiber 5, thereby completing the optical path process in the present invention. The micro liquid analysis detecting head for eliminating stray light 10 interference is connected to the receiving optical fiber 5 through the incident optical fiber 4 and a corresponding optical analysis device, such as visible light, spectrophotometric, fluorescent or spectral fluorescence detecting or analyzing equipment.

In the detecting head for micro liquid analysis capable of eliminating stray light interference, the angle of inclination of the carrier tray 3 with respect to the cross section of the housing 1 is such that the light reflected by the carrier tray 3 is deflected out of the light of the receiving optical fiber 5. The area 9 is received and the micro-liquid 6 to be detected on the transparent area is not slipped.

Although the embodiments of the present invention have been disclosed as above, they are not limited to the applications listed in the specification and the embodiments, and are fully applicable to various fields suitable for the present invention, and are easily accessible to those skilled in the art. The invention is not limited to the specific details and the details shown and described herein, without departing from the scope of the appended claims.

Claims

A detection head for micro liquid analysis capable of eliminating stray light interference, characterized in that it comprises:

a housing, which is a vertically arranged hollow cylindrical structure;

a carrier disk covering the top opening of the casing and disposed at a certain inclination angle along a cross-sectional direction of the casing to form an inclined table top for closing the top end of the casing, the carrier plate being provided with Placing a transparent area of the trace liquid to be tested;

a concentrating device, which is a light-transmitting body capable of collecting light, the concentrating device is movably connected to the inside of the casing in an adjustable distance from the carrier tray and separates the interior of the casing into two independent space;

a reflective sheet disposed above the carrier tray.
The detection head for micro liquid analysis capable of eliminating stray light interference according to claim 1, wherein a portion of the carrier plate outside the transparent region is coated with a hydrophobic layer to limit the liquid to be detected to a circular transparent The region forms a hemispherical droplet;

In addition, the transparent area of the carrier tray is set to a circle having a diameter of 1.2-2 mm, and the transparent area of the carrier disk has a thickness of 0.5-2 mm.
The detection head for micro liquid analysis capable of eliminating stray light interference according to claim 2, wherein a center of the circular transparent region of the carrier tray is overlapped with a center of the carrier tray and a center of the concentrating device is disposed at On the same vertical central axis.
The detection head for micro liquid analysis capable of eliminating stray light interference according to claim 1, wherein the reflection sheet is fixedly connected to a height adjustable bracket and the distance between the reflection sheet and the carrier tray is to be The height of the hemispherical droplets formed by the trace liquid is detected to correspond.
The detection head for micro liquid analysis capable of eliminating stray light interference according to claim 3 or 4, wherein a distance between the concentrating device and the carrier tray is such that the incident light passes through the concentrating device The central position of the circular transparent zone of the carrier disk passes through and passes through the liquid to be detected and is focused on the reflective sheet.
The detection head for micro liquid analysis capable of eliminating stray light interference according to claim 1, wherein a bottom end of the casing is provided for closing the bottom end of the casing and matching the inner diameter of the casing. A sealing plug is movably coupled to the housing by being embedded in the housing in a positionally adjustable manner.
The detection head for micro liquid analysis capable of eliminating stray light interference according to claim 6, wherein the concentrating device is movably connected to the housing in such a manner that the side wall of the sealing plug is along The inner wall of the housing extends inwardly from the connecting device, and the concentrating device is fixedly connected with the connecting device and interlocked with the sealing plug to adjust the distance between the concentrating device and the carrying tray.
The detection head for micro liquid analysis capable of eliminating stray light interference according to claim 7, wherein the sealing plug is respectively provided with an incident fiber and a receiving fiber penetrating through the sealing plug, wherein the incident fiber and the receiving fiber are respectively provided. Arranging respectively at opposite positions on both sides of the central axis of the sealing plug, the first end of the receiving fiber passing through the sealing plug is arranged as a light receiving area;

The micro liquid analysis detecting head for eliminating stray light interference is connected to the receiving optical fiber and the corresponding optical analysis device through the incident optical fiber.
The detection head for micro liquid analysis capable of eliminating stray light interference according to claim 2 or 8, wherein the carrier disk is inclined at an oblique angle of the cross-sectional direction of the casing to deflect light reflected by the carrier disk. The light receiving area of the receiving fiber is out and the micro liquid to be detected on the transparent area is not slipped, and the inclined angle of the carrying disk along the cross-sectional direction of the housing is less than or equal to 20 degrees.
The detection head for micro liquid analysis capable of eliminating stray light interference according to claim 2 or 8, wherein when the thickness of the carrier disk is 0.9-1.1 mm, the distance between the incident fiber and the receiving fiber is 0.3- At 0.5 mm, the optimal tilt angle of the carrier is 2-3 degrees.