WO2015199976A8 - Microdissection activée par une cible - Google Patents

Microdissection activée par une cible Download PDF

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Publication number
WO2015199976A8
WO2015199976A8 PCT/US2015/034930 US2015034930W WO2015199976A8 WO 2015199976 A8 WO2015199976 A8 WO 2015199976A8 US 2015034930 W US2015034930 W US 2015034930W WO 2015199976 A8 WO2015199976 A8 WO 2015199976A8
Authority
WO
WIPO (PCT)
Prior art keywords
biological sample
transfer film
target
selectively
reagent
Prior art date
Application number
PCT/US2015/034930
Other languages
English (en)
Other versions
WO2015199976A1 (fr
Inventor
Robert F. Bonner
Nicole Y. Morgan
Thomas J. Pohida
Philip G. MCQUEEN
Randall H. PURSLEY
John KAKAREKA
Original Assignee
The United States Of America, As Represented By The Secretary, Department Of Health And Human Services
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The United States Of America, As Represented By The Secretary, Department Of Health And Human Services filed Critical The United States Of America, As Represented By The Secretary, Department Of Health And Human Services
Publication of WO2015199976A1 publication Critical patent/WO2015199976A1/fr
Publication of WO2015199976A8 publication Critical patent/WO2015199976A8/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/08—Devices for withdrawing samples in the solid state, e.g. by cutting involving an extracting tool, e.g. core bit
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G01N2001/2833—Collecting samples on a sticky, tacky, adhesive surface
    • G01N2001/284—Collecting samples on a sticky, tacky, adhesive surface using local activation of adhesive, i.e. Laser Capture Microdissection

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne un procédé de retrait d'une cible à partir d'un échantillon biologique, consistant à : fournir à un échantillon biologique monté sur un substrat un réactif qui agit de manière sélective sur la cible dans l'échantillon biologique, le réactif comprenant un agent qui absorbe la lumière d'une longueur d'onde qui chauffe sélectivement le réactif; placer un film de transfert flexible ayant une surface inférieure et une surface supérieure opposée de telle sorte que la surface inférieure du film de transfert soit adjacente à l'échantillon biologique; appliquer un gradient de pression d'air uniforme sur le film de transfert flexible de façon à maintenir un contact thermique entre la surface inférieure du film de transfert et l'échantillon biologique; exposer l'échantillon biologique à la lumière de la longueur d'onde afin de chauffer sélectivement le réactif et de produire un changement dans le film de transfert, ce qui permet de faire adhérer sélectivement le film de transfert à la cible; et retirer de manière sélective la cible de l'échantillon biologique en retirant de l'échantillon biologique le film de transfert avec la cible mise à adhérer.
PCT/US2015/034930 2014-06-24 2015-06-09 Microdissection activée par une cible WO2015199976A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462016571P 2014-06-24 2014-06-24
US62/016,571 2014-06-24

Publications (2)

Publication Number Publication Date
WO2015199976A1 WO2015199976A1 (fr) 2015-12-30
WO2015199976A8 true WO2015199976A8 (fr) 2016-02-25

Family

ID=53484166

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2015/034930 WO2015199976A1 (fr) 2014-06-24 2015-06-09 Microdissection activée par une cible

Country Status (1)

Country Link
WO (1) WO2015199976A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106053165A (zh) * 2016-05-16 2016-10-26 常州大学 一种观察痰液黏蛋白微观结构的光学新方法
EP3833482A2 (fr) * 2018-08-07 2021-06-16 XMD Diagnostics, Inc Dispositifs et cartouches pour extraction de régions de bio-échantillon et de molécules d'intérêt
AU2019389908A1 (en) * 2018-11-29 2021-06-10 La Trobe University Method of identifying a structure
DE102020119764A1 (de) 2020-07-27 2022-01-27 Rheinisch-Westfälische Technische Hochschule Aachen (RWTH), Körperschaft des öffentlichen Rechts Vorrichtung und Verfahren zur Vorbereitung und Präparation von Gewebeproben

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US4745055A (en) 1985-05-07 1988-05-17 California Biotechnology Inc. Fused protein for enzyme immunoassay system
CA1340456C (fr) 1986-07-07 1999-03-23 Hubert J.P. Schoemaker Immunoglobulines chimeriques de rongeur/homme specifiques pour antigenes associes a des tumeurs
GB8626412D0 (en) 1986-11-05 1986-12-03 Clark M R Antibodies
US4856351A (en) * 1987-10-27 1989-08-15 Amoco Corporation Sample chamber and system for analyzing fluid inclusions
AU622426B2 (en) 1987-12-11 1992-04-09 Abbott Laboratories Assay using template-dependent nucleic acid probe reorganization
EP0425563B1 (fr) 1988-07-20 1996-05-15 David Segev Procede d'amplification et de detection de sequences d'acide nucleique
US5427930A (en) 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
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US5883144A (en) 1994-09-19 1999-03-16 Sentinel Products Corp. Silane-grafted materials for solid and foam applications
AT402203B (de) 1995-06-13 1997-03-25 Himmler Gottfried Dipl Ing Dr Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren
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Also Published As

Publication number Publication date
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