WO2015179996A1 - Utilisation de la protéine uch677 et du gène codant pour celle-ci à des fins de régulation de la croissance et du développement des plantes - Google Patents

Utilisation de la protéine uch677 et du gène codant pour celle-ci à des fins de régulation de la croissance et du développement des plantes Download PDF

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WO2015179996A1
WO2015179996A1 PCT/CN2014/000534 CN2014000534W WO2015179996A1 WO 2015179996 A1 WO2015179996 A1 WO 2015179996A1 CN 2014000534 W CN2014000534 W CN 2014000534W WO 2015179996 A1 WO2015179996 A1 WO 2015179996A1
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plant
seed
protein
rice
growth
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PCT/CN2014/000534
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Chinese (zh)
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王东辉
白书农
许智宏
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北京大学
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)

Definitions

  • the invention belongs to the technical field of plant molecular biology, and relates to the application of a UCH677 protein and a coding gene thereof for regulating plant growth and development.
  • heterosis is based on a combination of two different parents.
  • male sterility traits widely used in breeding work and production are mostly derived from natural mutations and their trait-transferred strains.
  • the source of male sterility traits is very limited and is a serious constraint to expand screening of hybrid combinations, especially applications.
  • all innovations with potential applications are protected by intellectual property rights. Therefore, the search for new ideas and methods for artificially controlling the size and yield of crop seeds with independent intellectual property rights has become an unavoidable and urgent problem to be solved in countries and regions that want to grasp the initiative of exploring the potential application of heterosis. one.
  • the genetic engineering method has some characteristics compared with the traditional method: the breeding cycle is shortened, the fertility is relatively stable, the environmental impact is small, the genotype is less dependent, and the environmental pollution is less.
  • the object of the present invention is to provide a UCH677 protein and a gene encoding the same for regulating plant growth and development.
  • the UCH677 protein is as follows (a) or (b):
  • the plant growth and development can be embodied in at least one of the following 1) -10):
  • the length of the plant is long.
  • the above application is embodied by: the higher the expression level of the UCH677 protein in the plant, the heavier the seed weight of the plant, and/or the larger the seed volume, and/or the longer the seed granule length, and/or The wider the seed granule width, and/or the thicker the seed granule thickness, and/or the more single ear seed number, and/or the higher the plant height, and/or the longer the plant leaf length, and/or the plant leaf width
  • the width, and/or the longer the plant panicle length; the lower the expression level of the UCH677 protein in the plant, the lighter the seed weight of the plant, and/or the smaller the seed volume, and/or the longer the seed granule length Short, and/or narrower the seed granule width, and/or the thinner the seed granule thickness, and/or the fewer the number of single ear seeds, and/or the shorter the plant height, and/or the shorter the plant leaf length, and/or Or the narrower the leaf width of
  • Vm plant leaf length growth or shortening
  • the selected plant species are reduced in seed weight, and/or reduced in seed volume, and/or reduced in seed length, and/or narrowed in seed width, and/or thinned in seed size, and/or single
  • the UCH677 is required when the number of spiked seeds is reduced, and/or the plant height is shortened, and/or the plant leaf length is shortened, and/or the plant leaf width is narrowed, and/or the plant ear length is shortened. Plants with lower protein expression were crossed as parents.
  • the method for cultivating a transgenic plant provided by the present invention may specifically be as follows (A) or (B):
  • a method of cultivating a transgenic plant having at least one of the following bl)-b lO) traits comprising the steps of: a) introducing a gene encoding the UCH677 protein into a plant of interest to obtain a transgenic plant expressing the gene encoding;
  • step b) obtaining, from the transgenic plant obtained in step a), a transgenic plant having at least one of the following bl) -blO) traits of interest compared to the plant of interest:
  • a method of cultivating a transgenic plant having at least one of the following dl) -dlO) traits comprising the steps of:
  • step c) obtaining, from the transgenic plant obtained in step c), a transgenic plant having at least one of the following dl) -dlO) compared to the plant of interest:
  • the above stringent conditions may be that the solution is mixed with a solution of 6x SSC, 0.5% SDS at 65 ° C, and then washed once with 2 x SSC, 0.1% SDS and P lx SSC , 0.1% SDS.
  • sequence 2 consists of 534 nucleotides, which is the coding sequence (ORF) of the t/CH677 gene; the sequence 2 encodes the protein shown by the sequence 1 in the sequence listing, and the sequence 1 consists of 177 amino acid residues.
  • the gene encoding the UCH677 protein is specifically introduced into the plant of interest by a recombinant expression vector.
  • the recombinant expression vector can be constructed using existing plant expression vectors.
  • the plant expression vector includes a dual Agrobacterium vector and a vector which can be used for plant microprojectile bombardment, and the like, such as pGr een 0029, pCAMBIA3301, pCAMBIA1300, pBI121, pBin1, pCAMBIA2301, P CAMBIA1301 - UbiN or other derivative plant expression vectors.
  • the plant expression vector may further comprise a 3 ' untranslated region of the foreign gene, ie, comprising a polyadenylation signal and any other DNA fragment involved in mRNA processing or gene expression.
  • the polyadenylation signal directs the addition of polyadenylation to the 3' end of the mRNA precursor.
  • any enhanced, constitutive, tissue-specific or inducible promoter such as cauliflower mosaic virus (CAMV) 35 S may be added before the transcription initiation nucleotide.
  • CAMV cauliflower mosaic virus
  • promoter ubiquitin gene Ubiquitin promoter (pUbi), stress-inducible promoter rd29A, etc., which can be used alone or in combination with other plant promoters; in addition, when the recombinant expression vector is constructed using the gene of the present invention, Using enhancers, including translational enhancers or transcriptional enhancers, these enhancer regions may be ATG start codons or contiguous region start codons, etc., but must be identical to the reading frame of the coding sequence to ensure proper translation of the entire sequence. .
  • the sources of the translational control signals and initiation codons are broad and may be natural or synthetic.
  • the translation initiation region can be from a transcription initiation region or a structural gene.
  • the recombinant expression vector used can be processed, such as a gene encoding a color-changing enzyme or luminescent compound that can be expressed in plants, and a resistant antibiotic marker. Or anti-chemical reagents, etc. Transformed plants can also be screened directly by stress without any selectable marker genes.
  • the promoter for initiating transcription of the t/CH677 gene in the recombinant expression vector is specifically an Actin promoter.
  • the recombinant expression vector is a recombinant plasmid obtained by inserting the UCH677 gene into a multiple cloning site of the PCAM23A vector; in one embodiment of the present invention, the multiple cloning site is specifically Xba I and Sa.
  • the expression of the gene encoding the UCH677 protein in the plant of interest may be any method which reduces the expression of the UCH677 gene in the plant of interest.
  • the recombinant expression vector carrying the UC 77 gene or the RNA interference expression vector of the t/CH677 gene is introduced into the plant of interest.
  • the plant cell or tissue can be transformed by using conventional methods such as Ti plasmid, Ri plasmid, plant viral vector, direct DNA transformation, microinjection, conductance, Agrobacterium-mediated transformation, and the transformed plant tissue is cultivated into Plant.
  • the plant may be a monocot or a dicot. Things.
  • the plant is specifically a monocotyledonous rice, such as a rice variety 1 1 .
  • the leaf length and the leaf width are each specifically a leaf length and a leaf width of the flag leaf.
  • Figure 1 shows the results of PCR identification of transgenic rice transformed into the overexpression vector pCAM23A-t/CH677o3 ⁇ 4.
  • lane M is the DNA molecular weight standard, and the bands are 5000, 3000, 2000, 1000, 750, 500, 300, 200 bp in order from large to small; lanes 1-7 are positively identified plants, and 8 are untransgenic wild plants.
  • Figure 2 shows real-time quantitative PCR detection of t/CH677 gene in transgenic rice transformed into recombinant expression vector pCAM23A-t/CH677o3 ⁇ 4.
  • WT indicates that the wild-type rice in the untransformed rice is 1 1; 677ox represents the transgenic rice transformed into the recombinant expression vector pCAM23A-t/CH677o3 ⁇ 4.
  • the expression level of t/CH677 gene in flower 1 of wild-type rice which was not transgenic was 1. * indicates a significant difference compared with WT (P ⁇ 0.05).
  • Figure 3 shows the phenotype of rice plants for each genetic material of t/CH677 gene.
  • WT is expressed as wild-type rice in the untransformed rice flower 11; 677ox indicates that the positive-positive generation is transferred into the over-expressing vector pCAM23 A-UCH677ox transgenic rice plants.
  • Figure 5 shows the rice panicle phenotype of the t/CH677 gene genetic material.
  • WT indicates that the wild-type rice in the non-transgenic rice is 1 1; 677ox represents the transgenic rice plant transformed into the overexpression vector pCAM23A-t/CH677o3 ⁇ 4.
  • Figure 6 shows the number of single-leaf seeds in rice of each genetic material of t/CH677 gene.
  • ⁇ 1 1 indicates the untransformed wild-type rice Zhonghua 11; 677ox expressed the transgenic rice plants into the overexpression vector pCAM23A-t/CH677o3 ⁇ 4, and * indicates significant difference compared with ⁇ 1 1 (P ⁇ 0.05).
  • Figure 7 shows the rice grain phenotype of each genetic material of t/CH677 gene.
  • WT indicates that the wild-type rice in the non-transgenic rice is 1 1; 677 ⁇ represents the transgenic rice plant transformed into the overexpression vector pCAM23A-t/CH677o3 ⁇ 4.
  • Figure 8 shows the statistical results of grain length, grain thickness and grain width of rice grains of t/CH677 gene.
  • A, B and C are grain length, grain width and grain thickness, respectively.
  • WT indicates that the wild-type rice in the non-transgenic rice was 1 1; 677ox expressed the transgenic rice transformed into the recombinant expression vector pCAM23A-t/CH677o3 ⁇ 4, and * indicates a significant difference compared with WT (P ⁇ 0.05).
  • Figure 9 shows the 1000-grain weight of rice seeds of each genetic material of the t/CH677 gene.
  • ⁇ 1 1 indicates that wild-type rice in the non-transgenic rice is in flower 11; 677ox represents the expression into the overexpression vector.
  • the transgenic rice plants of pCAM23A-t/CH677o3 ⁇ 4 showed a significant difference (P ⁇ 0.05) compared with ZH11.
  • Figure 10 shows the seed setting rate of rice seeds of each genetic material of t/CH677 gene.
  • ⁇ 11 indicates that wild-type rice in the non-transgenic rice was flower 11; 677ox expressed the transgenic rice plants into the overexpression vector pCAM23 A-UCH677ox.
  • the following examples are provided to facilitate a better understanding of the invention but are not intended to limit the invention.
  • the experimental methods in the following examples are conventional methods unless otherwise specified.
  • the test materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent stores. In the quantitative tests in the following examples, three replicate experiments were set, and the results were averaged.
  • pCAM23A carrier Beijing Dingguo Changsheng Biotechnology Co., Ltd. It is described in "Chi Zhengchang. Water meiosis gene OsSGOl function research and analysis. Yangzhou University, 2010, Master thesis” in the article.
  • the promoter located upstream of Xba I on the pCAM23 A vector is the Actin promoter.
  • Rice variety Zhonghua No. 11 purchased from the Crop Research Institute of the Chinese Academy of Agricultural Sciences; by the Chinese Academy of Agricultural Sciences, in 1979, Jingfeng No. 5 / Tetepu / Fujin was used for flower cultivation. It is described in "Ni Kuangchong. Rice Flower Culture New Seeds, Zhonghua No.11. Crop Variety Resources, 1989, 04".
  • the medium involved in the process of obtaining transgenic plants in the following examples is as follows:
  • KN0 3 (NH 4 ) 2 S0 4 KH 2 P0 4
  • MgS0 4 -7H 2 0 is dissolved in a beaker with 100 mL of water and then slowly poured into 1 while stirring, and there is no precipitation.
  • CaCl 2 '2H 2 0 is prepared with MgS (V7H 2 0; placed in a brown bottle
  • N6 vitamin pyridoxine hydrochloride VB6 1 mg/L 100 mg/L (lOOx)
  • Inositol 100 mg/L 10 g/L (lOOx)
  • the brown bottle is stored at 4 degrees, preferably 100 ml each time. Inositol is added directly to the medium. In the medium.
  • the amount of AAM is 1/10 of the trace amount of N6. It is only necessary to dilute the N6 trace 10 times.
  • the brown bottle is stored at 4 degrees, preferably 100 ml each time. Inositol is added directly to the medium when the medium is formulated.
  • resistance screening medium 1L poured on a disposable plate about 40-50 water
  • KT Preparation of 5 mg/ml KT: Weigh 100 mg of kinetin Kinetin, dissolve it with a small amount of 1 M KOH, and dilute to 20 ml with water. After filtration and sterilization, it was placed in a sterile vial and stored frozen at -20 °C.
  • Example 1 Acquisition and identification of 1/CH677 overexpressing transgenic rice plants
  • the t/CH (577 gene derived from rice Wryza.mtiva L.) involved in the present example, the cDNA sequence thereof is shown in sequence 2 in the sequence listing, and the sequence 2 is composed of 534 nucleotides;
  • the protein shown in SEQ ID NO: 1 (UCH677 protein), and SEQ ID NO: 1 consists of 177 amino acid residues.
  • PCR amplification was carried out using primers 23A677-F and 23A677-R using sequence 2 in the sequence listing as a template.
  • the PCR product was digested with restriction enzymes 3 ⁇ 4 ⁇ I and ⁇ 3 ⁇ 4 ⁇ ⁇ , and the target fragment was recovered and used in a restricted manner.
  • a large fragment of the pCAM23A vector backbone after Dicer Xba I and Sal I digestion was ligated to obtain a recombinant plasmid.
  • the recombinant plasmid into which the DNA fragment shown in SEQ ID NO: 2 in the sequence listing was inserted between the restriction sites Xba I and Sal I of the pCAM23A vector was designated as pCAM23A-t/CH677o3 ⁇ 4.
  • the promoter for transcription of the DNA fragment shown in SEQ ID NO: 2 in the sequence listing is the Actin promoter.
  • the young ears of the flower variety No. 1 in the rice variety 12-15 days after flowering were threshed, rinsed with water, soaked in 70% ethanol for 1-2 minutes, and then added with 1% (v/v) Tween20.
  • a 1.25% sodium hypochlorite aqueous solution (active chlorine content of 1.25% (w/v)) was immersed for 90 minutes for surface sterilization. (Stir well when sterilizing) Rinse with sterile water for 3-4 times, drain off the water for use. Water was squeezed on a sterile filter paper with tweezers and a scraper.
  • Rice immature embryos were placed on solid induction medium (B minimal medium) and callus was induced by dark culture at 26 °C. After about 5-7 days, the callus was peeled off, transferred to freshly prepared subculture medium (B basic medium), subcultured for about 5 days under the same conditions, and used for co-cultivation.
  • the dehulled rice mature seeds are first soaked in 70% ethanol for 1-2 minutes, then soaked for 30 minutes with 30%-40% sodium hypochlorite aqueous solution (active chlorine content 30%-40% (w/v)) for surface extinction.
  • Bacteria preferably on a shaker
  • Bacteria rinse 3-4 times with sterile water, place the seeds on sterile filter paper and blot them dry, then place them on mature embryo callus induction medium (B basic medium).
  • Dark culture at 26 ° C can be light culture, light culture grows fast).
  • the callus grown from the mature embryo scutellum was peeled off, transferred to mature embryo subculture medium (B basic medium), and subcultured under the same conditions. It will be subcultured every two weeks.
  • the callus cultured for 4-5 days and color yellowish granules was selected for co-cultivation.
  • the E. coli DH5a strain containing the recombinant expression vector pCAM23A-t/CH677o3 ⁇ 4 and pCAM23A empty vector constructed in the first step was inoculated into 5 ml LB (containing kanamycin 50 mg/L) liquid medium, and shaken at 37 ° C, 200 rpm. Cultivate overnight.
  • the recombinant plasmid was extracted according to the plasmid extraction kit of V-GE E.
  • Agrobacterium tumefaciens EHA105 was inoculated into 5 ml of YEP (streptomycin-containing Sm50 mg/L) liquid medium and shaken at 28 °C, 200 rpm overnight until the OD600 value was 0.4.
  • step III Repeat step III three times.
  • the scraped lawn is resuspended in YEB liquid medium and cultured at 28 ° C until the late log growth period; then 0.5 ml is transferred to 100 ml of the same YEB liquid medium for 2-3 h to an OD600 of about 0.5,
  • the cultured recombinant Agrobacterium was centrifuged at 4000 g for 10 minutes, and the pellet was suspended in 100 ml of AAM liquid medium to form a recombinant Agrobacterium suspension.
  • the callus was placed on a screening medium containing 25 mg/L Hygromycin for 14 days and then transferred to a screening medium containing 50 mg/L Hygromycin for further screening. 2 Monday generation. Most of the callus browned about 10 days after screening, and then re-growth of milky white resistant callus on the edge of the browned tissue. The choice generally lasts 6-8 weeks.
  • the milky yellow dense resistant callus was selected and transferred to a differentiation medium containing 50 mg/L hygromycin Hygromycin for 3 days. Then, the cells were cultured under light conditions of 16-20 h/d, light intensity of 100-120 ⁇ m ⁇ 1 ⁇ 2 s" 1 , and further differentiated into seedlings after 30-40 days.
  • the seedlings were transferred to a rooting medium and cultured for about two weeks. Choose a seedling with a height of about 10cm and a well-developed root. Wash the medium with warm water and transplant it in the greenhouse. Earth. The water surface is not submerged in seedlings. If it is fine, it needs to be shaded to survive in the seedlings (subject to spit water).
  • two transgenic vaccines having hygromycin resistance were finally obtained, that is, the rice plants which were transferred to the recombinant expression vector pCAM23A-t/CH677o3 ⁇ 4 and pCAM23A empty vector constructed in the first step).
  • Genomic DNA was extracted from the transgenic rice transformed into the recombinant expression vector pCAM23A-t/CH677 0 ; c, and the control plants transferred to the pCAM23A empty vector, respectively, from the generation obtained in step 1.
  • the primers 23 A677-F and 23 A677-R were used for PCR amplification, and the plants with the size of about 534 bp were identified as transformed into recombinant expression.
  • Step (1) Identification of the positive generation into the recombinant expression vector pCAM23A-t/CH677 0 c transgenic rice, transferred to the pCAM23A empty vector control plant, and the untransgenic rice variety Zhonghua 11, extracted from the leaves respectively RNA, reverse transcription to obtain cDNA, using this cDNA as a template, real-time fluorescent quantitative PCR amplification of cDNA of gene UCH677 with primers 677RT-F and 677RT-R, with GAPDH as internal reference, primers as GAPDH-RT-F and GAPDH -RT-R.
  • 677RT-R 5 '-ATCCTTGGCCTCTGT ATCGC -3 ' (reverse complement of positions 314-333 of sequence 2);
  • GAPDH-RT-F 5 ' - AAGCC AGC ATCCTATGATC AGATT-3 ';
  • GAPDH-RT-R 5'-CGTAACCCAGAATACCCTTGAGTTT-3 O
  • the QPCR experiment was carried out using ABI Prism 7000 real-time PCR system and power SYBR Green I mixing kit.
  • Real-time PCR assay pre-denaturation at 95 °C for 30 s; 95 °C for 5 s, 60 °C for 34 s, 40 cycles.
  • Power (2- ⁇ ) was used to calculate the template ratio between different samples to indicate the relative expression of different genes.
  • the positive expression was transferred to the recombinant expression vector pCAM23A-t/CH677 0 ; c of the transgenic rice transgenic plant, the wild type rice variety Zhonghua No. 1 No. 1, and Example 1
  • the obtained control plants transferred to the pCAM23A empty vector were used as experimental materials.
  • the seeds of each experimental material were sown in a petri dish for germination (80-100 seeds per plant material), and the seedlings after germination were transplanted in pots, and then transferred to the Datian of the suburbs of Beijing for growth. Analyze and identify the following aspects. Ten genetic tests were randomly selected for each genetic material. For quantitative data, the average of 10 strains was obtained.
  • seed length, thickness and width Observe and compare the seed phenotypes of each genetic material in the same period, and quantitatively analyze the seed volume (seed length, thickness and width) and 1000-grain weight.
  • seed volume seed length, thickness and width
  • 1000-grain weight were basically the same as those of the untransgenic wild-type rice variety Zhonghua No.1, and there was no statistical difference. . 4.
  • the seed setting rate of the grain on the rice ears of each genetic material was statistically analyzed.
  • the seed setting rate real grain number / total grain number x l00%
  • the real grain refers to the rice grain
  • the total grain number is the real grain number plus the empty grain number (no rice empty shell).
  • the present invention up-regulates the expression level of UCH677 protein and its coding gene in rice by gene overexpression technology, which can lead to the following phenotype of rice seeds: compared with wild type rice seeds, seeds The weight increases, the volume increases (the grain length, the width, and the thickness increase), and the number of single ear seeds increases.
  • the over-expressed plants have higher plant height and longer leaf length than the wild type. Leaf width and ear length increased.
  • the present invention lays the foundation for finding a simpler idea and method for creating high-yield properties.

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Abstract

L'invention concerne une utilisation d'une protéine UCH677 et d'un gène codant pour celle-ci à des fins de régulation de la croissance et du développement des plantes. Ladite utilisation correspond à une utilisation d'une protéine constituée de la séquence d'acides aminés représentée par la séquence 1 dans le listage des séquences ou d'un gène codant pour celle-ci à des fins de régulation d'au moins l'un des facteurs 1) à 10) suivants de la croissance et du développement des plantes : 1) le poids des semences ; 2) la taille des semences ; 3) la longueur des grains de semence ; 4) la largeur des grains de semence ; 5) l'épaisseur des grains de semence ; 6) le nombre de semences dans un seul épi ; 7) la hauteur de la plante ; 8) la longueur des feuilles de la plante ; 9) la largeur des feuilles de la plante ; et 10) la longueur des épis de la plante. Des expériences ont démontré que, pendant le développement du riz, une régulation à la hausse des niveaux d'expression de la protéine UCH677 dans le riz peut amener les semences de riz à présenter le phénotype suivant : par comparaison avec une semence de riz de type sauvage, le poids de la semence augmente, le volume augmente et, dans le même temps, le nombre de semences dans un seul épi augmente ; et, en outre, en termes de phénotype de la plante, par rapport au type sauvage, la hauteur de la plante présentant une telle surexpression est plus grande. Cela nous permet de poser les bases de la découverte de processus de réflexion menant à des procédés de création plus simples d'un caractère correspondant à un rendement élevé chez les plantes cultivées.
PCT/CN2014/000534 2014-05-27 2014-05-27 Utilisation de la protéine uch677 et du gène codant pour celle-ci à des fins de régulation de la croissance et du développement des plantes WO2015179996A1 (fr)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112795588A (zh) * 2019-10-28 2021-05-14 中国农业科学院生物技术研究所 OsSGD1蛋白在调控水稻籽粒大小中的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006138005A2 (fr) * 2005-05-10 2006-12-28 Monsanto Technology, Llc Genes et leurs utilisations pour l'ameliorations de plantes
CN103003432A (zh) * 2010-07-19 2013-03-27 巴斯夫植物科学有限公司 具有增强的产量相关性状的植物和用于产生该植物的方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006138005A2 (fr) * 2005-05-10 2006-12-28 Monsanto Technology, Llc Genes et leurs utilisations pour l'ameliorations de plantes
CN103003432A (zh) * 2010-07-19 2013-03-27 巴斯夫植物科学有限公司 具有增强的产量相关性状的植物和用于产生该植物的方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE NCBI [O] 8 June 2010 (2010-06-08), TANAKA, T. ET AL.: "Os04g0546400 [Oryza Sativa Japonica Group].", XP055240785, Database accession no. NP_001053472.1 *
MOON, Y.K. ET AL.: "Structure and Expression of OsUBP6, an Ubiquitin-Specific Protease 6 Homolog in Rice (Oryza Sativa L.", MOLECULES AND CELLS, vol. 28, no. 5, 30 November 2009 (2009-11-30), pages 463 - 472 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112795588A (zh) * 2019-10-28 2021-05-14 中国农业科学院生物技术研究所 OsSGD1蛋白在调控水稻籽粒大小中的应用
CN112795588B (zh) * 2019-10-28 2022-06-14 中国农业科学院生物技术研究所 OsSGD1蛋白在调控水稻籽粒大小中的应用

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