WO2015131830A1 - Dispositif de culture d'organismes photosynthétiques - Google Patents

Dispositif de culture d'organismes photosynthétiques Download PDF

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Publication number
WO2015131830A1
WO2015131830A1 PCT/CN2015/073651 CN2015073651W WO2015131830A1 WO 2015131830 A1 WO2015131830 A1 WO 2015131830A1 CN 2015073651 W CN2015073651 W CN 2015073651W WO 2015131830 A1 WO2015131830 A1 WO 2015131830A1
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WIPO (PCT)
Prior art keywords
carrier
support
photobioculture
support member
liquid
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PCT/CN2015/073651
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English (en)
Chinese (zh)
Inventor
朱振旗
王琳
陈昱
罗少敬
吴洪
陈传红
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新奥科技发展有限公司
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Publication of WO2015131830A1 publication Critical patent/WO2015131830A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices

Definitions

  • the invention relates to a photobioculture device.
  • Photosynthetic microorganisms or plant cells can synthesize organic matter and inorganic substances such as hydrogen and methane using solar energy, water and simple minerals.
  • Microalgae is one of the typical representatives. It is a kind of individual light-energy autotrophic/constrained, single-cell/simple multi-cellular organism with wide distribution, wide variety, high photosynthetic efficiency, fast growth and adaptability. Strong and so on.
  • the annual fixed CO 2 of microalgae accounts for about 40% of the global net photosynthetic yield.
  • it is rich in high-value antioxidant natural pigments such as lipids, hydrocarbons, proteins and soluble polysaccharides. Therefore, microalgae are environmentally friendly, energy and Health care and other fields have received much attention.
  • the existing microalgae cultivation methods are mainly "large water body culture", and the culture system mainly includes an open runway pool and a closed/semi-closed reactor.
  • the runway pool is mostly elliptical or circular shallow pool.
  • the algae liquid in the pool is mostly 20-30cm deep.
  • the algae liquid is continuously stirred by the drum-shaped stirring paddle, so as to realize the circulating flow of the whole algae liquid, so that the algae liquid can be Microalgae cells and nutrients are evenly distributed.
  • the aquaculture production of the runway pool has not been ideal. On the one hand, as the microalgae cells grow, the turbidity of the algae liquid gradually increases.
  • the closed/semi-closed reactors are mostly in the form of tubes, columns and plates with a height of more than 2 meters. The light utilization efficiency of the reactor increases with the increase of its height, but at the same time, the pressure of the liquid (algae liquid) received in the middle and bottom of the reactor also increases, resulting in a substantial increase in reactor manufacturing cost and gas supply energy consumption.
  • the light-receiving surface of the reactor cannot be adjusted correspondingly with the change of the natural light source, and the height of the reactor is restricted by the conditions of the material (especially the light-receiving surface material) and the light transmittance and high strength.
  • the space for improving the efficiency of the reactor light utilization is limited.
  • U.S. Patent No. 2011/0217764 A1 discloses a method of attaching algal cells to a rope a large-body culture method in which the surface of the rope and the rope wound thereon are continuously exposed to the nutrient solution and sunlight to grow algae cells on the rope, which eliminates the conventional liquid-based culture medium.
  • the solid culture method is adopted, that is, the algal cells are mainly grown on the carrier.
  • the above-mentioned culture device has insufficient space utilization, and the "split" effect is limited, and it is not possible to adjust the spectroscopic light (corresponding to cell light receiving) according to the change of the external environment (light intensity) and the different growth stages of the algae cells.
  • Literature Growing microalgae as aquaculture feeds on twin-layers a novel solid-state photobioreactor and Attached cultivation technology of microalgae for efficient biomass feedstock production discloses a "double layer" microalgae immobilized culture system, which is mainly composed of a "resource” layer, The "matrix” layer and the circulatory system are constructed.
  • the “resource” layer and the “matrix” layer can be formed into a thin sheet and arranged vertically.
  • the circulation system continuously supplies nutrient solution to the “resource” layer, and the “matrix” layer is attached with microalgae cells on one side, and the other side is directly attached to “
  • the resources are on both sides of the layer to allow the algae cells attached thereto to obtain nutrients for growth.
  • the "double layer” structure is not necessary, and the algae cells on the “matrix” layer are not really fixed with the “matrix” layer, and the algae cells are easily separated from the "matrix” layer during the breeding process.
  • the system cannot adjust the spectroscopic (equivalent to cell light) according to the changes of the external environment (light intensity) and the different growth stages of algae cells, and the various culture surfaces ("resource” layer and "matrix” layer) in the system block each other.
  • the apparatus disclosed in the document Attached cultivation technology of microalgae for efficient biomass feedstock production is isolated from the outside to grow algae cells in a gas environment containing CO 2 .
  • the light intensity, temperature, and air CO 2 partial pressure controllability are low, and the construction of a glass greenhouse (to provide a closed environment in the literature) has a high cost, so the device is not easy to industrialize and scale up applications.
  • a photobioculture apparatus comprising: at least one support frame; and at least two spaced apart carriers capable of adsorbing photobiocells, nutrient solution and photobioculture liquid, each carrier being suspended from the support frame on.
  • the support frame has a first support member, each carrier being rotatably suspended from the first support member and/or slidably suspended along the first support member in an axis perpendicular to the axis of the first support member.
  • the carrier is a sheet-like member, all of the sheets on the same first support member are connected in series by a guide, and the guide members are spaced apart from the first support member.
  • the carrier is a rod that is slidably suspended along the first support.
  • the carrier is made of one or more of glass fiber, nylon, cotton, hemp, carbon fiber, synthetic fiber, sponge, plastic foam, metal, synthetic plastic.
  • the carrier comprises a base and a cover covering the base, the cover being made of an adsorbent material capable of adsorbing photobiocells, nutrient solution and photobioculture fluid.
  • the carrier is a concave-convex structure or a structure provided with a through hole.
  • a circulation device for providing a nutrient solution or a photo-bio-culture solution for a carrier.
  • a circulation device includes: a liquid supply member disposed on a support frame, the liquid supply member including a liquid inlet port and a liquid outlet port disposed toward the carrier; and an infusion device in fluid communication with the liquid inlet port of the cloth liquid member.
  • the support frame comprises: two support rods spaced apart from each other and arranged parallel to each other; the two ends are respectively movably connected to the first support members of the two support rods, wherein the carrier is suspended from the first support member;
  • the utility model comprises: a driving component that drives the first support to move back and forth along the support rod.
  • a plurality of support frames are provided spaced apart, and the arrangement direction of the plurality of support frames is perpendicular to the arrangement direction of the carriers on the same support frame.
  • the cultivation of photo-organisms on a carrier suspended from a support frame avoids the disadvantage of cultivating the cells in the cell body to cause insufficient light to be exposed to the light, so that the photo-organisms on the carrier are sufficiently exposed to light.
  • the at least two carriers disposed apart from each other can reduce mutual light-shielding between the carriers, and further enable the light organism on the carrier to be sufficiently exposed to light.
  • the photobioculture device of the present invention has a better spectroscopic effect, thereby increasing the yield of photobiomass.
  • the photo-bioculture device of the present invention has a simple structure, low cost, and high standing The body expands the space and the space utilization rate is high, so the device can be used for large-scale farming, thereby increasing the overall yield of photobio.
  • the carrier is suspended from the first support member in a rotatable manner perpendicular to the axis of the first support member of the support frame, so that the carrier can adjust the setting direction of the carrier according to the direction of illumination and the needs of the photon organism itself at different growth stages, ie
  • the angle between the carriers can be adjusted, thereby improving the light splitting effect, further avoiding mutual shading between the carriers, so that more carriers can be cultured while satisfying the illumination requirement.
  • the carrier surface can be uniformly received by rotating the carrier, thereby improving the light splitting effect, thereby improving the photobioproduct yield.
  • the sheet member when the carrier is a sheet member, the sheet member can be rotated by the guiding member connected to the carrier, so that the surface of each sheet member having the largest surface area is always completely received by light (ie, without mutual shielding), thereby The photobiological cells that adsorb the surface can be sufficiently exposed to light.
  • the carrier is slidably suspended on the first support member, so that the carrier can adjust the spacing of the carrier according to the direction of illumination and the needs of the photobio own at different growth stages, that is, the spacing between the carriers can be adjusted, thereby improving the spectroscopic effect. Further mutual shading between the carriers is avoided, and more photobiocells can be cultured while satisfying the illumination requirements.
  • the suspension pitch of the carrier can be adjusted according to, for example, the light intensity, the direction of illumination, and the light demand of different photo organisms, thereby improving the utilization of illumination and cultivating more photobiota while satisfying the requirements.
  • the carrier can be supplemented with a nutrient solution or a new photobiocell can be introduced while replenishing the nutrient solution, thereby illuminating the light Sufficient while ensuring that photobiocells grow faster.
  • the carrier may be partially or completely immersed in a pool body containing a nutrient solution or a photo-bio-culture liquid (a nutrient solution containing photobio cells) under the carrier, thereby serving as a carrier. Introduce new photobiocellular cells while replenishing nutrient solution or replenishing nutrient solution.
  • a nutrient solution or a photo-bio-culture solution is sprayed onto the carrier by a cloth liquid member provided on the support frame, thereby introducing a new photo-bioreactor while replenishing the nutrient solution or replenishing the nutrient solution.
  • Figure 1 is a schematic view of a first embodiment of the photobioculture apparatus of the present invention
  • FIG 2 is a schematic view of the light bio-culture device shown in Figure 1 with a guide member 6;
  • Figure 3 is a schematic view of the carrier in the photobioculture apparatus shown in Figure 1 positioned according to a direction of illumination;
  • Figure 4 is a schematic illustration of the carrier in the photobioculture apparatus shown in Figure 1 positioned according to another direction of illumination;
  • Figure 5 is a schematic view of a second embodiment of the photobioculture apparatus of the present invention.
  • Figure 6 is a schematic view showing the addition of a cloth liquid member and a guide member to the photobioculture device shown in Figure 5;
  • Figure 7 is a schematic view of a third embodiment of the present invention.
  • Figure 8 is a schematic view of a fourth embodiment of the present invention.
  • Figure 9 is a partial schematic view of the first support member and the carrier shown in Figure 8.
  • Figure 10 is a partial schematic view of a fifth embodiment of the present invention.
  • a first embodiment of a photobioculture apparatus of the present invention comprises: at least one support frame 2; and at least two spaced apart carriers 4 capable of adsorbing photobiocells, nutrient solution and photobioculture fluid, Each carrier 4 is suspended from a support frame 2.
  • the carrier 4 is preferably made of a material having high water retention, toughness, and durability, and it is not toxic or slightly toxic to the cultured photobiological cells.
  • the carrier 4 is made of one or more materials of glass fiber, nylon, cotton, hemp, carbon fiber, synthetic fiber, sponge, plastic foam, metal, synthetic plastic to adsorb photobio cells, nutrient solution and photobio Farming fluid.
  • the carrier 4 comprises a base and a covering portion covering the base, the base being made of a material having a certain hardness, and the covering portion being made of an adsorbing material capable of adsorbing photo-biological cells, nutrient solution and photo-aquaculture liquid, in particular
  • the adsorbing material is one or more materials of glass fiber, nylon, cotton, hemp, carbon fiber, synthetic fiber, sponge, plastic foam, metal, synthetic plastic, thereby covering the cover portion on the base to form absorbable light
  • the carrier 4 of the biological cells, the nutrient solution and the photo-bio-culture liquid which has a certain hardness and can be kept flat when suspended.
  • the carrier 4 A rectangular sheet made of white cheesecloth, 1.5 meters long, 0.3 meters wide, and about 0.001 meters thick. The two narrow sides are respectively bound by a plastic rod (not shown) having a length of 0.3 meters and a diameter of about 0.05 meters. To maintain the flat state of the carrier.
  • the photo-organism is cultivated on the carrier 4 suspended on the support frame 2, thereby avoiding the disadvantage that the light culture in the cell body is insufficient to receive light in the lower portion of the cell body, so that the photo-organism located on the carrier 4 is sufficiently exposed to light.
  • the at least two carriers 4 which are spaced apart from each other can reduce mutual light-shielding between the carriers 4, further allowing the light organisms on the carrier 4 to be sufficiently exposed to light.
  • the photobioculture device of the present invention has a better spectroscopic effect, thereby increasing the yield of photobiomass.
  • the photo-bioculture device of the present invention has a simple structure, low cost, and high space utilization rate due to a high dimensional expansion space, so the device is used for large-scale cultivation, thereby improving the overall yield of photobio.
  • each of the support frames 2 is provided with two support rods 7 spaced apart from each other and arranged in parallel with each other, and a first support member 3 which is movably coupled to the two support rods 7, respectively.
  • the carrier 4 is a sheet member, and is suspended from the first support member 3 in a rotatable manner perpendicular to the axis of the first support member 3.
  • the midpoint of one narrow side of the carrier 4 and the first support member 3 are Rotatingly fixed, and the carrier 4 is rotatable along a vertical virtual axis passing through the fixed point, and the distance between two adjacent carriers 4 on the same first support member 3 is 0.5 m.
  • the first support member 3 is coupled to the two support rods 7 and supports the carrier 4 in a manner to suspend the carrier 4.
  • the first support 3 can be a rigid rod or a flexible cord.
  • the support rod 7 has better strength to achieve stable support.
  • the number of the support frames 2 is not limited thereto, and an appropriate number of support frames 2 may be selected in a case where it is ensured that the carrier 4 is sufficiently exposed to light and space is allowed per day.
  • the carrier 4 can be driven to rotate by the addition of the guide member 6.
  • the guide 6 can be a rigid rod or a flexible cord.
  • all the sheets (i.e., the carrier 4) on the same first support member 3 are connected in series by the guide member 6, and the guide member 6 and the first support member 3 are spaced apart from each other. It should be understood that due to the guide 6
  • the drive carrier 4 can be rotated, so that the guide 6 is arranged parallel to the first support 3 and is not in the same vertical plane as the first support 3, which is perpendicular to the horizontal plane.
  • the same side vertex of the narrow sides of the first support member 3 of the two narrow sides of the carrier 3 on the same first support member 3 is connected to the guide member 6 so as to uniformly adjust the angle of the carrier 4. That is, the rotation of the carrier 4 is uniformly controlled, whereby the adjustment of the angle of the carrier 4 (the direction of the light receiving surface) can be achieved by the pulling guide 6.
  • the guide 6 is constructed of about 0.01 meters of twine.
  • the form, connection and position of the guide member 6 and the carrier 1 are not limited thereto, as long as the rotation of the carrier 4 can be controlled by the guide member 6.
  • a plurality of guide members 6 may be provided, so that the rotation of the drive carrier 4 is more convenient and labor-saving.
  • the carrier is suspended from the first support member 3 in a rotatable manner perpendicular to the axis of the first support member 3 of the support frame 2, so that the carrier 4 can be in accordance with the illumination direction (refer to Figs. 3 and 4), the light intensity and the photobio itself.
  • the need to adjust the orientation of the carriers at different stages of growth i.e., to adjust the angle between the carriers), thereby improving the beam splitting effect, and more carriers 4 can be hung to meet the illumination requirements to increase throughput.
  • the carrier surface can be uniformly received by rotating the carrier, thereby improving the light splitting effect, thereby improving the photobioproduct yield.
  • the carrier when the carrier is a sheet member, the sheet member can be rotated by a guiding member connected to the carrier, so that the surface of the sheet member having the largest surface area is sufficiently exposed to sunlight and does not block each other, thereby adsorbing the surface.
  • the photobiocellular cells can obtain a suitable light intensity, thereby increasing overall yield.
  • the embodiment further includes a circulation device for supplying the nutrient solution or the photo-bio-culture liquid (the nutrient solution containing the photo-biological cells) to the carrier 4, and the circulation device includes a driving unit for driving the first support member 3 to reciprocate along the support rod 7.
  • a cell body 1 located below the carrier 4.
  • the pool body 1 is a brick square shallow pool, lined with PVC material, and has a length and a width of 5 meters and a height of 0.6 meters.
  • the pool body 1 contains a nutrient solution or photo-bio-culture liquid rich in nutrients required for the growth of microalgae, and the nutrient solution or photo-bio-culture liquid is 0.1-0.2 m deep.
  • the support rods may be supported on the ground, for example, on the outside of the pool body 1, and may of course be located inside the pool body 1 or on the rim of the pool body 1.
  • the structure of the pool body 1 is not limited to the form shown in FIG. 1, and a plurality of pools corresponding to a single piece or a plurality of carriers on one first support member may be provided.
  • Body 1 and each of the pool bodies is provided with a nutrient solution or a photo-bio-culture solution rich in nutrients for the growth of microalgae and a disinfectant. Therefore, nutrient solution or photo-bio-culture liquid can be supplemented according to the consumption of nutrients and the like in different pool bodies, so as to better control the cultivation of photo-biomass, and improve productivity and benefit.
  • the nutrient solution in each cell body can also be collected through a pipeline and uniformly replenished.
  • the circulation device provides the nutrient solution or the photo-bio-culture solution (containing the nutrient solution and the photo-biological cells) for the carrier 4, which is determined according to the specific kind of the photo-organism and the adsorption performance of the carrier 4, that is, in the embodiment, the cell body
  • the nutrient solution or photobioculture liquid contained in 1 is determined according to the specific type of photobiological organism and the adsorption performance of the carrier 4.
  • the cultured photobiocell can be stably adsorbed on the carrier 4, it will not be detached from the carrier 4 into the cell body 1 after being immersed in the liquid in the cell body 1 (or only a small amount of photobiocellular cells are detached from the carrier 4) ), the nutrient solution is contained in the cell body 1, which is understood to be solid culture. If the cultured photobiological cells are detached from the carrier 4 into the cell body 1 after being immersed in the liquid in the cell body 1 (or a relatively large amount of photobio cells are detached from the carrier 4), the cell body 1 contains the photobioculture liquid.
  • the carrier 4 In order to replenish the photobiological cells flowing into the photobioculture liquid each time the carrier 4 is immersed, and maintaining the photobioculture amount on the carrier 4, it can be understood as liquid culture.
  • the above-mentioned solid culture nutrient solution does not completely contain photobio cells, but the content of photobio cells in the nutrient solution in the solid culture is much less than the content of photobio cells in the liquid culture.
  • the pool body 1 contains a nutrient solution, that is, a solid culture method.
  • the first support member 3 is made of hemp rope having a diameter of about 0.05 m, a total length of 5 m, and both ends are connected to a hemp rope having a diameter of about 0.035 m.
  • the support rod is provided with a slide rail and is provided with a pulley that can slide up and down with respect to the slide rail.
  • the hemp rope is connected to the pulley and slides on the slide rail via the motor drive pulley.
  • the motor drives the first support member 3 to move downward to immerse the carrier 4 in the nutrient solution to supplement the nutrient solution on the carrier, or to immerse the carrier 4 in the photo-bio-culture solution to replenish the nutrient solution and simultaneously introduce new light.
  • the motor drives the first support member 3 to move upward to disengage the carrier 4 from the liquid surface of the nutrient solution or photobio-culture solution to be completely exposed to sunlight.
  • the present embodiment is to remove the carrier 4 from the liquid surface of the nutrient solution;
  • the carrier 4 is removed from the liquid surface of the photo-aquaculture liquid.
  • the first support member 3 may be made of bamboo, wood, synthetic plastic, nylon, hemp, dip metal, and may preferably be made of a water-resistant, anti-corrosive material with good rigidity. Or flexibility. It can be understood that when the first support member 3 is a flexible member, the carrier 4 suspended thereon may cause the first support member 3 to slightly bend. In this case, it can be understood that each carrier 4 is perpendicular to the first support member 3. The axis is rotatably suspended in an axis perpendicular to the first support member 3 when it is not bent, i.e., the axis when each carrier 4 is not suspended.
  • the carrier 4 since the carrier 4 is suspended from the first support member 3, its axis of rotation is parallel to its direction of gravity. In other words, without being limited to this expression, the carrier 4 can be rotated according to the direction in which the sunlight is irradiated during suspension to avoid mutual shading between the carriers.
  • the carrier 4 is immersed in a culture vessel containing Scenedesmus, and a mixed gas of air and CO 2 (in which the CO 2 content is 1-2%) is introduced, and after 4 days of cultivation under natural light conditions, the carrier 4 is removed. And hanging on the first support member 3, the distance between the adjacent two carriers 4 on the same first support member 4 is controlled to be 0.5 meters. Every 4 minutes, the first support member 3 is slowly moved toward the pool body 1 by the action of the motor, so that the upper carrier 4 is immersed in the nutrient solution in the pool body 1. After the carrier 4 is completely submerged, the motor drives the first support member 3 and The upper surface of each of the carriers 4 is placed under the sunlight, so that the upper and lower movements of the first support members 3 are staggered.
  • the guide member 6 is pulled so that the surface of the carrier 4 having the largest area sufficiently sees light, so that the microalgae cells obtain a suitable light intensity.
  • the microalgae cells on the carrier 4 can be harvested every 5-10 days, and the microalgae cells remaining on the carrier 4 after the harvest are used as "seeds" and used directly for a new round of culture.
  • the carbon source (mixture containing CO 2 , pure CO 2 gas, flue gas, carbonate, bicarbonate, etc.) and phosphorus source can be directly added to the pool.
  • Phosphate Phosphate
  • nitrogen source and the like
  • a disinfectant for preventing and controlling pests and diseases, and then the nutrients, disinfectants, and the like which are added are evenly distributed in the cell body 1 by stirring or aeration.
  • the structure of the pool body is not limited to the form shown in FIG. 1 , and the partition body is arranged in the pool body 1 to divide the pool body 1 into a plurality of sub-pool bodies, and the nutrient/disinfectant may be separately supplemented in the sub-pool body, or The nutrient solution in the cell body 1 is collected and uniformly replenished. Or set multiple pool bodies, one pool body corresponding to one or more carrier settings.
  • the moving speed of the first support member 3 and the dwell time in daylight are adjusted and set according to actual weather conditions.
  • a light intensity feedback system can also be provided. The light intensity feedback system detects the direction and intensity of the illumination and automatically controls the movement of the guide member 6 according to the detection result, thereby automatically controlling the rotation angle of the carrier 4.
  • the carrier 4 in this embodiment can also be moved along the axial direction of the first support member 3, thereby adjusting the spacing of the adjacent carriers 4 according to the illumination and the growth of the algae itself, so as to improve the spectroscopic effect and satisfy More photobiocells can be cultured under the premise of illumination requirements.
  • the material, structure, size, number, and the spacing and movement period of the first support member 3 in the cell body 1, the support rod 7, the first support member 3, the carrier 4, the guide member 5, and the circulation device in this embodiment are not limited thereto. . That is to enable the "draw" nutrient solution, so that the microalgae cells can grow photosynthesis on the carrier containing the nutrient, and adjust the carrier angle (similar to the louver) according to the change of light intensity and illumination direction, Any embodiment that allows the microalgae cells to obtain suitable growth conditions is within the scope of protection.
  • the first support member 3 of the present embodiment is made of bamboo raft.
  • the circulation device comprises a cloth liquid member 8 (shown in FIG. 6) and an infusion device which are arranged on the support frame 2.
  • the cloth liquid member 8 comprises a liquid inlet port and a liquid outlet opening provided toward the carrier 4, and the liquid material member 8 is advanced.
  • the liquid port is in communication with the infusion device.
  • the infusion device can pump the nutrient solution or the photo-bio-culture liquid in the cell body 1 into the liquid inlet of the liquid-discharging member 8 (in the embodiment, the nut body liquid is contained in the cell body 1, that is, the solid culture is performed, so the infusion solution
  • the device pumps the nutrient solution in the cell body 1 into the liquid inlet of the liquid discharge member 8.
  • the cloth liquid member 8 is a cloth liquid pipe
  • the infusion device is a water pump.
  • the cloth liquid member 8 is made of a plastic tube having an inner diameter of about 1 cm, The liquid outlet having a diameter of 0.5-1 mm is used, and the nutrient solution or the photo-bio-culture solution is uniformly and slowly dropped on the carrier 4 having the microalgae cells via the liquid outlet of the cloth liquid member 8. It will be understood that the number and position of the liquid outlets are set in accordance with the number of carriers 4 and the spacing between the two carriers 4 to enable supply of sufficient nutrient solution to the photobiocells on the carrier 4.
  • the carrier 4 is made of a 50 mesh nylon mesh, and the cell body 1 is a photo-bio-culture liquid (nutrient solution and microalgae cells), and the photo-bio-culture liquid is evenly dropped on the carrier via a water pump and a cloth liquid piece (The carrier does not need to be pre-cultured for 4 days in the algal solution as shown in Examples 1 and 2, so that the photo-bio-culture solution forms a flowing thin liquid layer on the carrier, so that the photo-bioreactor fully sees light, performs light and action.
  • a photo-bio-culture liquid nutrient solution and microalgae cells
  • the concentration of microalgae cells in the cell body 1 reaches 2-5 g/L
  • the concentration of some microalgae cells in the cell body 1 to the microalgae cells in the pool is 1-2 g/L, and a new round of culture is started.
  • each of the carriers 4 is a rod slidably suspended along the first support member 3, that is, the carrier 4 is movable in the axial direction of the first support member 3.
  • the first support member 3 is made of a coarse rope of 0.035 meters
  • the carrier 4 is made of a white fiber rope (1.5 meters long and 0.02 meters thick) on the same first support member 3.
  • the distance between two adjacent carriers is 0.05 meters, and a total of eight first support members 3 are provided, and the distance between each adjacent two first support members 3 is 0.05 meters.
  • the cultured algae species is Chlorella vulgaris, and the first support member 3 is slowly moved to the cell body 1 by the action of the motor every 4 minutes to make the carrier (the carrier does not need to be precultured for 4 days in the algae liquid as shown in Example 1).
  • the motor drives the first support member 3 and the upper surface of each carrier 4 to be placed in the sunlight, and the photo-biology liquid A flowing thin liquid layer is formed on the carrier 4 so that the microalgae cells sufficiently see the light, perform light and action, and thus reciprocate, and the up and down movement of each of the first supports is staggered.
  • the first support member 3 (made of hemp rope, that is, a flexible member) can be pulled between the carriers 4 on the different first support members 3.
  • the relative position changes, so that the microalgae cells obtain a suitable light intensity.
  • concentration of microalgae cells in the pool reaches 2-5g/L, collect some microalgae cells from the pool to the pool.
  • the medium microalgae cell concentration was 1-2 g/L, and a new round of culture was started.
  • the spacing between adjacent carriers 4 on the first support member 3 of the same root can be adjusted according to the changes in light intensity, illumination direction and microalgal cell growth stage, so that the microalgae cells can obtain suitable growth conditions, thereby improving Light utilization to increase microalgae production
  • the first support member 3 is directly fixed to the support rod 7.
  • the first support member 3 is made of bamboo rafts having a thickness of about 0.03 meters, and the carrier 4 is made of a white coarse cotton rope (1.5 meters long and 0.02 meters thick), and the two carriers 4 on the same first support member 3
  • the pitch is 0.05 m, and a total of 8 first support members 3 are provided, and the distance between each adjacent two first support members 3 is 0.05 m.
  • the cloth liquid member 8 is made of a plastic tube having an inner diameter of about 1 cm, and is provided with a liquid outlet having a diameter of 0.5 to 1 mm, and the nutrient solution flows in the cloth liquid member 8 and is dropped on the carrier 4 via the liquid outlet.
  • the algae species are cultured as freshwater chlorella.
  • the relative position between the carriers on the different first support members can be changed by pulling the first support member, thereby obtaining suitable light intensity for the microalgae cells.
  • the adjustment function of the first support member to the carrier is adjusted and set according to actual weather conditions; the first support member can be automatically adjusted by being connected to the light intensity feedback system.
  • the photobioculture apparatus of the present invention is not limited to the above structure.
  • the carrier 4 is a concave-convex structure or a structure provided with a through hole, that is, the surface of the carrier 4 has depressions and projections, or the carrier 4 is provided with a through hole.
  • Both the concavo-convex structure and the structure provided with the through holes can improve the liquid holding ability of the carrier, that is, the carrier 4 can maintain more of the liquid therein without being separated from the carrier 4 by its own weight when suspended. Thereby, the frequency of use of the circulation device can be reduced to reduce the cost.
  • each carrier 4 can be suspended from the first support member 3 in a rotatable manner perpendicular to the axis of the first support member 3, or can be slidably suspended on the first support member 3 along the first support member 3. Or slidably suspended along the first support member 3 on the first support member 3 while being suspended from the first support member 3 in the above-described manner. Up, that is, simultaneous movement of rotation and sliding.
  • the choice of the type of exercise depends on the type of photobiological culture, the condition of the light, etc., and is not limited to a certain type of exercise, so that the light energy can be effectively utilized and the yield can be improved.
  • a moving mechanism for driving the carriage movement may be further included to better cause the carrier to follow the movement of the sun to form a larger light receiving surface.
  • the moving mechanism can drive the support frame to rotate about the axis of the circular cell body.
  • the setting of the cell body 1 can be based on whether or not it is necessary to recover the nutrient solution dropped from the carrier 4, and whether or not the application is based on the above method of taking the nutrient solution in the cell body 1 in the cell body 1.
  • the device can be built in the original runway pool, natural pit, pool, and lakeside, making its fixed investment low. The device does not need to transport a large amount of water (nutrient solution/photosynthetic microbial culture solution), thereby reducing the water consumption, operating energy consumption, equipment and facility investment in the aquaculture process.
  • the carrier 4 in the device can slowly and vertically enter and leave the nutrient solution, has low resistance, and can utilize the gravitational potential energy and other natural energy sources, so the total energy consumption is low.
  • the relative arrangement of the cell body and the carrier is not limited to the structure described in the above embodiment. Specifically, one pool body can be provided for each carrier. The cell bodies corresponding to the respective carriers may be connected to each other or isolated from each other.
  • the manner of moving the first support member along the support rod is not limited to the above embodiment, for example, a pulley is provided on the support rod, the rope is fixed at both ends of the first support member, and the rope passes through the pulley to utilize the lever
  • the principle moves the first support down or up.
  • the driving member that drives the rope to move or drives the first support member to move along the support rod may be, for example, an electric motor, and the power source is preferably an environmentally friendly energy source such as solar energy or tidal energy.

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Abstract

La présente invention porte sur un dispositif de culture d'organismes photosynthétiques, comprenant au moins un cadre de support (2) et une pluralité de supports (4). Les supports (4) sont espacés et sont capables d'adsorber des cellules d'organismes photosynthétiques, une solution nutritive et une solution de culture d'organismes photosynthétiques. Chaque support (4) est suspendu sur le cadre de support (2), et est utilisé pour cultiver un organisme photosynthétique. L'espacement et les angles des au moins deux supports espacés l'un de l'autre sont réglables, ce qui permet à l'organisme photosynthétique sur les supports de recevoir une lumière suffisante.
PCT/CN2015/073651 2014-03-04 2015-03-04 Dispositif de culture d'organismes photosynthétiques WO2015131830A1 (fr)

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CN104069522A (zh) * 2014-06-18 2014-10-01 新奥科技发展有限公司 一种治理微藻固定化培养中污染的方法
CN104109635A (zh) * 2014-06-18 2014-10-22 新奥科技发展有限公司 一种防治微藻固定化培养中污染的方法
CN104069521A (zh) * 2014-06-18 2014-10-01 新奥科技发展有限公司 一种治理微藻培养过程中污染的方法
CN104195045A (zh) * 2014-08-14 2014-12-10 新奥科技发展有限公司 一种光生物细胞的培养方法
CN105385563B (zh) * 2014-09-03 2017-11-17 新奥科技发展有限公司 一种阵列式光生物反应器的分光方法
CN104328032A (zh) * 2014-10-30 2015-02-04 国家开发投资公司 表面生长式光合微生物培养单元、培养系统及培养方法
CN104328030A (zh) * 2014-10-30 2015-02-04 国家开发投资公司 夹心结构的表面生长式培养板、系统及培养方法
CN104480014B (zh) * 2014-11-20 2017-12-19 新奥科技发展有限公司 微藻固定化培养方法及装置
CN104593222B (zh) * 2015-01-14 2017-01-11 新奥科技发展有限公司 一种微藻的固定化养殖装置和方法
CN106754239B (zh) * 2016-11-16 2019-02-26 新奥科技发展有限公司 一种微藻固体培养系统
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