WO2015093566A1 - Deodorant and method for producing same - Google Patents

Abstract

[Problem] To provide a safe deodorant that maintains a high deodorizing effect over an extended period of time and is nontoxic to plants and animals, and a method for producing the same. [Solution] Rice bran is fermented by effective microorganisms, and a deodorant is made from a liquid collected from the fermented rice bran. The effective microorganisms are at least one among bacteria of the genus Pediococcus, yeast cells, microorganisms of the genus Bacillus, streptococci, and staphylococci. The above-mentioned liquid contains a component having deodorizing activity against the odor of amines. This component is at least one among lactic acid, acetic acid, propionic acid, butyric acid, and succinic acid. The above-mentioned liquid also contains a component having deodorizing activity against the odor of acids. This component is at least one among spermidine, spermine, and putrescine. The above-mentioned liquid also contains a component having deodorizing activity against the odor of aldehydes.

Description

Deodorant and production method thereof

The present invention relates to a deodorant made from a product obtained by fermenting rice bran and a method for producing the deodorant.

Conventionally, efforts have been made to reduce unpleasant odors caused by dust, septic, sweat, manure, livestock, poultry, pets, sewage, sludge, etc. A strong fragrance derived from a natural product or artificially synthesized makes it difficult to feel an unpleasant odor. In addition, a method in which odor is adsorbed by a solid substance such as activated carbon or a polymer gel is also used.

On the other hand, as a method for more positively removing odors, a method for reducing bad odors using microorganisms has been proposed, and deodorizing raw garbage and activated sludge using microorganisms such as Bacillus and Enterobacter. Deodorant (for example, Patent Document 5, Patent Document 5, Patent Document 5, Patent Document 5, Patent Document 5, Patent Document 5, Patent Document 5, Patent Document 5, Patent Document 5, Patent Document 5, Patent Document 1, 2, 3, 4) , A method for reducing malodor such as garbage using microorganisms belonging to the genus Isachenchia or Pichia (Patent Document 6), a deodorizing liquid such as a toilet containing a group of useful microorganisms and their metabolites from livestock urine (Patent Document 7) In addition, deodorizers using lactic acid bacteria (Patent Documents 8, 9, and 10) have been proposed.

As for enzyme utilization techniques, practical utilization methods have been proposed, such as utilizing the activity of enzymes extracted from natural products such as papain and pepsin for peptide synthesis (Non-Patent Documents 1 to 3). ).

Japanese Unexamined Patent Publication No. 7-222789 Japanese Patent Laid-Open No. 7-222790 JP 7-222791 A JP-A-8-59418 JP-A-11-104222 Japanese Patent Application Laid-Open No. 11-346761 JP 2001-187126 A JP 2005-512591 A JP 2010-111782 A JP 2011-30737 A

However, a method that suppresses unpleasant odors with strong fragrances cannot be said to be a fundamental solution because odors exist as they are, and there are individual differences in preferences regarding fragrances, so strong fragrances themselves can cause discomfort. There are drawbacks. In addition, with regard to the application of activated carbon, polymer gel, or microcapsule technology often found in commercially available deodorants, it only traps odors and does not decompose and remove odors. There is a limit.

In addition, with regard to the deodorizing effect using microorganisms, it seems that odor-causing molecules are decomposed by the enzyme activity of microorganisms, but most of the methods use microorganisms as they are, or after culturing, or both. In such a state, since most of the enzyme produced by the microorganism is retained in the microbial cell and efficient contact with the causative molecule as a substrate is limited, such an enzyme exerts a sufficient effect. I don't want to be. In addition, garbage collection and treatment plants including raw garbage, livestock,
At present, no effective and long-lasting deodorant has been found for odors such as poultry manure.

The present invention has been made in view of the above problems of the background art, and provides a safe deodorant that maintains a high deodorizing effect over a long period of time and has no toxicity to animals and plants, and a method for producing the same. The purpose is to do.

The present invention is a deodorant made of a liquid obtained by fermenting rice bran with useful microorganisms and collecting it from the fermented rice bran. The useful microorganism group is at least one of Pediococcus bacteria, yeast cells, Bacillus microorganisms, streptococci and staphylococci. The rice bran may be defatted rice bran.

The liquid contains a component having a deodorizing activity with respect to the odor of amines, and is, for example, at least one acid of lactic acid, acetic acid, propionic acid, butyric acid, succinic acid and the like. The amines that generate odor are trimethylamine, ammonia and the like.

Further, the liquid contains a component having a deodorizing activity against an acid odor, and is, for example, a polyamine which is a base derived from rice bran, which is at least one of spermidine, spermine, putrescine and the like. The acid generating odor is formic acid, acetic acid, isovaleric acid and the like.

The liquid contains a component having a deodorizing activity against the odor of aldehyde. For example, at least one base of spermidine, spermine, putrescine, and / or lactic acid, acetic acid, propionic acid, butyric acid, succinic acid It is at least one acid among acids. The aldehyde generating odor is formaldehyde, acetaldehyde or the like.

The liquid contains an enzyme, and the enzyme is at least one of lipase, amylase, protease, peptidase and sucrase produced by, for example, a Bacillus microorganism. Alternatively, the liquid is at least one of cellulase, hercellulase, xylanase, serbase, amylase, pectinase, lignase, arabinase, etc. produced by yeast.

The deodorant is prepared by adjusting the liquid obtained from the fermented rice bran to pH 3.0 to pH 6.0.

In the present invention, a useful microorganism group containing at least one of Pediococcus bacteria, yeast cells, Bacillus microorganisms, streptococci, and staphylococci is mixed with rice bran and heated at 40 to 60 ° C. for 8 to 15 hours. Fermented, then fermented at a temperature lower than the temperature and longer than the time, removed the liquid from the fermented rice bran, recovered the removed liquid, treated the liquid, the deodorant Is a method for producing a deodorant. The means for reducing the moisture content may be a vacuum dryer.

The deodorant is prepared by subjecting the liquid obtained from the fermented rice bran to a treatment for removing unnecessary substances. The treatment for removing unnecessary substances is, for example, dialysis, filtration, centrifugation and the like. In addition, you may sterilize by an autoclave.

The deodorant may be produced by subjecting the components removed in the drying process of the fermented rice bran to a liquid.

The deodorant of the present invention and the method for producing the deodorant can produce a useful microorganism group and rice bran, which is an inexpensive natural component, as a material by a simple production method, have a high deodorizing effect, and effectively reduce the odor component. It can be continuously removed, decomposed or modified. Moreover, the deodorant of the present invention is not toxic to plants and animals and can be used safely in various applications.

It is process drawing which shows the manufacturing method of the deodorizer of one Embodiment of this invention.

Hereinafter, embodiments of the present invention will be described. The deodorant 10 of this embodiment is made using a liquid obtained by collecting rice bran adjusted in water with a useful microorganism group and collecting the fermented rice bran. As the rice bran, defatted rice bran is used. Useful microorganism groups are Pediococcus bacteria, yeast cells, Bacillus microorganisms, streptococci and staphylococci.

The deodorant 10 includes a component having a deodorizing activity against the odor of amines such as ammonia and trimethylamine. For example, lactic acid, acetic acid, propionic acid, butyric acid, succinic acid, etc. having polar substance adsorption ability are used. It is an acidic substance. Further, the deodorant 10 includes a component having deodorizing activity against odors of organic acids such as acetic acid, isovaleric acid, formic acid, lactic acid, propionic acid, butyric acid, and succinic acid. For example, spermidine derived from rice bran, Basic substances such as spermine and putrescine. In addition, since the deodorizer 10 contains a basic substance and an organic acid, it has a high deodorizing activity against odors of aldehydes such as formaldehyde and acetaldehyde.

Further, the deodorant 10 contains an enzyme having a deodorizing activity against odors generated by fats and proteins. For example, lipases, amylases, proteases, peptidases, sucrases, etc. produced by Bacillus microorganisms, and cellulases, hercellulases, xylanases, serbiases, amylases, pectinases, ligases, arabinases, etc. produced by yeasts.

The deodorant 10 is adjusted to pH 3.0 to pH 6.0. In addition, even if it contacts or is ingested by a human body, a pet, livestock, a plant, it does not show toxicity.

Next, the manufacturing method of the deodorant 10 of this embodiment is demonstrated based on FIG. First, an appropriate amount of water is added to defatted rice bran and mixed to adjust it to an appropriate water content (30 to 40% by mass, for example, about 35% by mass) (s1). Cells, Bacillus microorganisms, streptococci and staphylococci) are mixed and further mixed (s2).

In this state, it is fermented for a certain time at a predetermined room temperature. For example, fermentation is performed at 40 ° C. to 60 ° C. for 8 hours to 15 hours, and thereafter, fermentation is performed at a temperature lower than the above temperature for a time longer than the above time. Preferably, it is fermented at 50 ° C. for 12 hours and then fermented at 35 ° C. for 48 hours (s3).

Next, the liquid is removed from the fermented rice bran with a vacuum dryer, for example, a conical dryer, until the water content becomes several mass%, for example, 6 mass% (s4). The components removed here are returned to the liquid and collected to make the deodorant 10, and the dried rice bran is used as the feed 12 or fertilizer.

Deodorant 10 obtained by treating fermented rice bran may be subjected to dialysis or sterilization filtration, high-temperature sterilization, etc. as necessary to remove odors and nutrients derived from the medium. The deodorant 10 may be used without being diluted as it is, but it is preferably used after being diluted with water or a buffer solution for keeping the pH constant. The dilution is preferably performed up to 2000 times, more preferably up to 1000 times. The pH is not particularly limited, but is preferably pH 3.0 to pH 6.0, more preferably pH 4.5 to pH 5.5, in order to suppress contamination and growth of various bacteria. If there is an insoluble matter, it may be removed by dialysis, filtration with a membrane such as filter paper or ultrafiltration membrane, centrifugation or the like. Further, it may be used after being sterilized by an autoclave or the like. As long as the deodorizing effect is not inhibited, various functional compounds such as antibacterial agents and fungicides, aromatic compounds and the like may be used in combination.

The deodorizing mechanism such as decomposition or adsorption of the deodorant component in the deodorant 10 of this embodiment is as follows. Pediococcus microorganisms included in the group of useful microorganisms produce bacteriocin, which is a proteinous antibacterial substance, and have a function of suppressing Salmonella, Escherichia coli Staphylococcus aureus, and the like. In addition, Pichia microorganisms are a kind of yeast having strong bactericidal properties, and their action suppresses the growth of microorganisms and suppresses malodors generated by the growth of microorganisms. In addition, by the enzymes produced by these useful microorganisms, fats and proteins are decomposed to decompose odorous substances or their original substances into odorless substances such as amino acids and glycerin or to alter them.

Furthermore, since the deodorizer 10 contains both an acidic substance and a basic substance, both acidic and basic substances are captured by acid-base interaction. Aldehydes are supplemented by interactions such as the formation of Schiff bases with basic substances, the substances captured in this way are neutralized, and general polar substances are also added to the water layer by their affinity with water. Deodorized by dissolving in. Due to these functions, the deodorant 10 has a powerful odor removal function and can be used for a wide range of purposes.

Next, how to use the deodorant 10 will be described. The method of using the deodorant 10 is not limited and can be used by various means. For example, it is filled in a spray container or a sprayer such as a trigger type and sprayed in the air containing an odor or an odor source. In particular, when sprayed or sprayed on an odor source, not only the deodorizing effect is shown in a short time but also the effect is sustained. Alternatively, it may be included in a cloth, sponge, etc., and the portion where the odorous material is adhered may be wiped off and deodorized at the same time. As a method for treating odor-containing air on a certain scale, in a processing apparatus for bubbling odor-containing air into the liquid of the deodorant 10 or spraying the odor-containing air in a shower form. It is also possible to treat the deodorizer 10 by making it pass through, for example, by passing it through.

Alternatively, the deodorant 10 may be adsorbed on a zeolite / nonwoven fabric or absorbed in a water-containing gel to carry the deodorant 10 and be contacted with air containing odor to treat the odor. The deodorant 10 may be bonded to the surface of the silica particles, and the silica particles may be contacted with an odor substance in a liquid phase or a gas phase to treat the odor. The place where the deodorant 10 is used is not limited to a sealed space such as a refrigerator, a toilet, sewage, a drainage facility, or a car interior, but may be an open space. Various odors, for example, organic substances generated by human activities, microorganisms such as mold, odors from animals, tobacco, food waste, toilets, cloth products, chemicals, etc., odors from excrement of livestock such as pets, pigs and poultry This is effective.

Since the deodorant 10 of this embodiment is affected by proteolytic enzymes, the deodorant 10 is stored avoiding contamination with microorganisms such as proteolytic enzymes or bacteria that produce and secrete proteolytic enzymes. That is desirable. Moreover, it is confirmed that the deodorizing effect of the deodorant 10 that has passed for two years is maintained and stable by confirmation of alteration by the examples described later, and can be stored at room temperature for a long time. However, it is desirable to store in a cool and dark place to avoid performance degradation and alteration.

According to the deodorant 10 of this embodiment, rice bran, which is an inexpensive natural component, can be made as a material by a simple manufacturing method, has a high deodorizing effect, and decomposes the odor component effectively and continuously. can do. In addition, there are the following effects. For example, a high deodorizing effect can be exhibited by a simple operation of spraying in air containing odor or spraying or spraying directly on an odor source. Moreover, not only the deodorizing effect is shown in a short time, but the effect can be maintained. It is not toxic to plants and animals, has a pH of 3.0 to 6.0, is easy to handle, and can be safely used in various applications by spraying with a general pressure sprayer or trigger sprayer. . As long as the deodorizing effect is not inhibited, it can be used in combination with various functional compounds such as antibacterial agents and fungicides, and fragrances that are aromatic compounds. The deodorant 10 is easy to add a fragrance and has little influence on various deodorant sensors. The deodorant 10 has a stable deodorizing effect over a long period of time and does not deactivate even by heat treatment, and thus can be stored at room temperature for a long time. In addition, because it contains various enzymes, it can decompose food components such as fats and oils, proteins, and sugars, and can be used as an organic compound degrading agent having a deodorizing function in detergents and various other applications. .

In addition to the liquid obtained by fermenting rice bran, the deodorant of the present invention may use a liquid prepared by blending the synthetic components of the above substances at a predetermined mixing ratio. The form is not limited.

Hereinafter, embodiments of the present invention will be described. The method for producing the deodorant 10 according to the present invention includes the following microorganisms: Pediococcus pentosaceus, Pediococcus acidilactici, Picia farinosa, Desella bulas 12 kg of soil containing at least one of Bacilli, Streptococci, Staphylococci, 30 kg of rice bran, 1 kg of soybean powder, and 14 Mix with liter of water. The mixture is then heated at 50 ° C. for 12 hours, after which the temperature is lowered to 35 ° C. and maintained at this temperature for 48 hours, after which the mixture is dried to a moisture content of about 6% by weight to form a stock culture.

Next, 500 kg of rice bran is mixed with 1.4 kg of the above preserved culture. Water is added to raise the water content to 35% by weight, the mixture is spread on a layer of 10 cm to 15 cm, covered with a plastic film to prevent water evaporation and fermented for 8 days. After 8 days, the plastic film is removed, and the fermented rice bran is dried to a moisture content of about 7-9% by weight using a container rotary vacuum dryer (conical dryer, manufactured by Shinko Environmental Solution Co., Ltd.). The evaporated water and the like were recovered by returning to the liquid component. The liquid thus obtained is the deodorant 10.

When the components of the deodorant 10 comprising the liquid obtained in the above process were analyzed, the total amount of nitrogen (TN) was 14 mg / l, the total amount of phosphoric acid (TP) was 12 mg / l, and the total amount of potassium (TK). It was 10 mg / l, copper was less than 0.1 mg / l, zinc was less than 0.1 mg / l, the carbon nitrogen ratio was 54 mg / l, and the pH was 3.8. Furthermore, as components, organic acids such as lactic acid, acetic acid, propionic acid, butyric acid and succinic acid, alcohols such as isoamyl alcohol, and polyamines derived from rice bran such as spermidine, spermine and putrescine were contained.

Next, the deodorant 10 which is the product of the present invention was evaluated for deodorizing power of ammonia, which is an odor component, by the detector tube method. In the test method, the deodorant 10 is diluted 500 times with purified water. Next, 5 ml of diluted deodorant 10 is poured into a petri dish, placed in a 5 liter Tedlar bag, and 3 liters of ammonia with an initial concentration of 100 ppm is added. Thereafter, the ammonia concentration was measured over time with a gas detector tube (detector tube model number: No. 3La ammonia, Gastec Co., Ltd.). The results are shown in Table 1.

According to Table 1, it turned out that the deodorizer 10 of the invention of this application has high ammonia deodorizing power.

Next, with respect to the deodorant 10 which is the product of the present invention, the deodorizing ability of trimethylamine, which is an odor component, was evaluated by the detector tube method. First, the deodorant 10 is diluted 500 times with purified water. Next, 5 ml of the diluted deodorant 10 is poured into a petri dish, placed in a 5 liter Tedlar bag, and 3 liters of trimethylamine having an initial concentration of 28 ppm is added. Thereafter, the concentration of trimethylamine was measured over time with a gas detector tube (detector tube model number: No. 180 amines, Gastec Co., Ltd.). The results are shown in Table 2.

According to Table 2, it turned out that the deodorizer 10 of the invention of this application has high trimethylamine deodorizing power.

Next, for the deodorant 10 which is the product of the present invention, the deodorizing power of acetic acid, which is an odor component, was evaluated by the detector tube method. First, the deodorant 10 is diluted 500 times with purified water. Next, 5 ml of the diluted deodorant 10 is poured into a petri dish, placed in a 5 liter Tedlar bag, and 3 liters of acetic acid having an initial concentration of 50 ppm is added. Thereafter, the concentration of acetic acid was measured over time with a gas detector tube (Gastech). The results are shown in Table 3.

According to Table 3, it turned out that the deodorizer 10 of the invention of this application has a high acetic acid deodorizing power.

Next, about the deodorizer 10 which is the invention of this application, the deodorizing power evaluation of the isovaleric acid which is an odor component was performed by the gas chromatograph method. First, the deodorant 10 is diluted 500 times with purified water. Next, the diluted deodorant 10 is directly injected into a 500 ml Erlenmeyer flask, and an ethanol solution of isovaleric acid is dropped to a prescribed initial concentration of 38 ppm and sealed. After 2 hours, sampling is performed with a syringe and gas chromatograph (GC) measurement is performed. The blank test concentration (a) after 2 hours and the sample test concentration (b) after 2 hours were measured by gas chromatography, and the reduction rate (%) was calculated according to the following formula (1).
Reduction rate (%) = {(ab) / a} × 100 (1)
The results are shown in Table 4.

According to Table 4, it turned out that the deodorizer 10 has high isovaleric acid deodorizing power.

Next, the deodorant 10 which is the product of the present invention was evaluated for deodorizing power of formaldehyde which is an odor component by a detector tube method. First, the deodorant 10 is diluted 500 times with purified water. Next, 5 ml of diluted deodorant 10 is poured into a petri dish, placed in a 5 liter Tedlar bag, and 3 liters of formaldehyde having an initial concentration of 15 ppm is added. Thereafter, the concentration of formaldehyde was measured over time with a gas detector tube (Gastech). The results are shown in Table 5.

According to Table 5, it turned out that the deodorizer 10 has high formaldehyde deodorizing power.

Next, with respect to the deodorant 10 which is the product of the present invention, alteration over time was confirmed by a sensory test. The test method consists of 5 panel members (Tokyo Foods Co., Ltd.) immediately after dilution of the solution obtained by diluting the deodorant 10 500 times with purified water (Target 1) and after 2 years of manufacture (Target 2). A sensory test based on human sensations was conducted by the Tokyo Food Technology Research Laboratories (Hygiene Association) to confirm alteration.

For Subject 1 and Subject 2, all five panel members evaluated that there was no abnormality in both odor and color. Thereby, it turned out that it has high preservability over a long period of time.

Next, a local irritation test (skin) for mice was performed on the deodorant 10 which is the product of the present invention. The test method is as follows. First, 0.1 g of the deodorant 10 extract diluted 500-fold with purified water is suspended in 100 ml of sterilized purified water and allowed to stand at 35 ° C. for 12 hours as a coating sample. Using mice (ddy system, 3 males), the waist and back (about 6 cm ² ) of the mice are shaved 24 hours before application. Using a glass rod on the waist and back of the sample for application, approximately 0.4 ml is applied once. Immediately after the application, 1 hour, 24 hours, 48 hours, and 72 hours later, signs of erythema and edema were observed. As a result, no abnormality (erythema and edema) was observed in the skin of the mouse. Thereby, it turned out that the deodorizer 10 has no toxicity with respect to the skin of a mouse | mouth.

Next, an acute toxicity test (oral) on mice was performed on the deodorant 10 which is the product of the present invention. In the test method, first, a solution obtained by diluting the deodorant 10 500 times with purified water is used as a sample for administration. Mice (ddy system, male, 5 animals) are fasted for 4 hours before administration, and an administration sample is forcibly administered once into the stomach using an oral sonde needle. The dose is equivalent to 30 ml of sample for administration per kg body weight. After administration, the mice were observed for abnormalities for 1 week, but no abnormalities were found in the mice. Thereby, it turned out that the deodorizer 10 does not have the acute toxicity with respect to the oral administration of a mouse | mouth.

10 Deodorant 12 Feed

Claims (10)

1. A deodorant made from a liquid obtained by fermenting rice bran with a useful microorganism group and collected from the fermented rice bran, wherein the useful microorganism group comprises Pediococcus bacteria, yeast cells, Bacillus microorganisms, streptococci and A deodorant characterized by being at least one of grape-like fungi.
2. The said liquid contains the component which has the deodorizing activity with respect to the odor of amines, The said component is at least one of lactic acid, acetic acid, propionic acid, butyric acid, and succinic acid. Odorant.
3. The deodorizer according to claim 1, wherein the liquid contains a component having a deodorizing activity against an acid odor, and the component is at least one of spermidine, spermine and putrescine.
4. The deodorizer according to claim 1, wherein the liquid contains a component having a deodorizing activity with respect to an aldehyde odor, and the component is a base or an acid.
5. 5. The liquid according to claim 1, wherein the liquid contains an enzyme, and the enzyme contains at least one of lipase, amylase, protease, peptidase, and sucrase produced by a Bacillus microorganism. Deodorant.
6. The deodorizer according to any one of claims 1 to 4, wherein the liquid contains at least one of cellulase, hercellulase, xylanase, serbiase, amylase, pectinase, lignase, and arabinase produced by yeast.
7. The deodorant according to claim 5 or 6, wherein the liquid obtained from the fermented rice bran is adjusted to pH 3.0 to pH 6.0.
8. A useful microorganism group containing at least one of Pediococcus bacteria, yeast cells, Bacillus microorganisms, streptococci, and staphylococci is mixed with rice bran and fermented at 40 to 60 ° C. for 8 to 15 hours, and then A deodorant that is fermented at a temperature lower than the temperature and longer than the time, removes the liquid from the fermented rice bran, collects the removed liquid, and treats the liquid to form the deodorant. Manufacturing method.
9. The method for producing a deodorant according to claim 8, wherein the deodorant is produced by subjecting the liquid obtained from the fermented rice bran to a sterilizing filtration.
10. The method for producing a deodorant according to claim 8, wherein the deodorant is produced by subjecting the component removed in the fermented rice bran drying step to a liquid.

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