WO2015068896A1 - Novel micractinium inermum nlp-f014 strain and use thereof - Google Patents

Abstract

The present invention relates to novel Micractinium inermum capable of producing biofuel and a use thereof. Novel Micractinium inermum NLP-F014 KCTC 12491BP microalgae according to the present invention can be very useful as a biomass fuel since the microalgae have a higher biomass concentration and total lipid content than other conventional microalgae and C16-C18 fatty acids occupy most of the measured lipid.

Description

Novel Micronium Inummum NLP-F014 Strains and Uses thereof

The present invention relates to novel microphone to produce biofuels below Tiny moth inner stop (Micractinium inermum) NLP-F014 KCTC 12491BP relates to microalgae and their use.

In addition to rising costs of fossil fuels as well as increased carbon dioxide as a cause of global warming, bioenergy has been spotlighted as an energy source to replace fossil fuels.

Accordingly, researches on bioenergy are being actively conducted all over the world, and microalgae have recently been spotlighted as the main target materials for biofuel research.

Microalgae are photosynthetic organisms that synthesize oxygen and produce oxygen using carbon dioxide, water, and solar energy.

Such microalgae may immobilize carbon dioxide and have high protein and lipids, and thus may be utilized as biomass for food, feed or fuel production. Specifically, lipids, sugars or proteins as primary metabolites of microalgae may be used as a source of various biofuels such as biodiesel, bioethanol or biogas, and vitamins, carotenoids, and secondary metabolites of microalgae, High value-added substances such as polysaccharides can be used as functional foods, natural pigments, medicinal substances, animal feeds, aquaculture feeds and the like.

In this regard, microalgae are recognized as the best materials for bioenergy unlike land plants because of their excellent carbon dioxide reduction effect, no political and social problems related to food resources, and high production yields. It is true.

Accordingly, the present inventors completed the present invention by first separating and identifying MICractinium inermum NLP-F014 KCTC 12491BP, which is a new microalgae rich in biomass and suitable for use as a biofuel.

Accordingly, an object of the present invention is to provide a micromass rich Micractinium inermum NLP-F014 KCTC 12491BP microalgae.

Another object of the present invention is to provide a raw material for a biomass fuel including Micractinium inermum NLP-F014 KCTC 12491BP microalgae or a culture solution thereof according to the present invention.

In order to achieve the object of the present invention as described above, the present invention provides a biomass-rich Micractinium inermum NLP-F014 KCTC 12491BP microalgae.

In one embodiment of the present invention, the microalgae biomass concentration of 4 ~ 6 g / L, biomass productivity of 0.5 ~ 1 g / L / day, specific growth rate is 1.0 ~ 1.4 / day and / or Lipid productivity may be between 0.1 and 0.5 g / L / day.

In one embodiment of the present invention, the microalgae may have a total lipid content of 35 to 45%.

In addition, the present invention provides a biomass fuel comprising the Micractinium inermum NLP-F014 KCTC 12491BP microalgae or a culture thereof according to the present invention.

In addition, the present invention provides a method for producing biofuel using Micractinium inermu m NLP-F014 KCTC 12491BP microalgae or a culture thereof according to the present invention.

The novel Micractinium inermum NLP-F014 KCTC 12491BP microalgae according to the present invention has a higher biomass concentration and total lipid content than other conventional microalgae, and most of the components in the measured lipids are C16. Since fatty acids in the ~ C18 region occupy, there is an effect that can be very usefully used as a biomass fuel.

1 is a Micractinium inermum This shows the ribosome DNA sequence of NLP-F014.

Figure 2 is a result showing the molecular lineage of the microorganism Micractinium inermum ( Micractinium inermum ) NLP-F014 of the present invention.

Figure 3 is a result showing the biomass concentration change of the new strain of the present invention.

Figure 4 is a result showing the total nitrogen concentration change of the new strain of the present invention.

5 is a result showing the total phosphorus concentration change of the new strain of the present invention.

Figure 6 shows the lipid content of the new strain of the present invention.

The present invention is characterized by providing a novel Micractinium inermum NLP-F014 KCTC 12491BP microalgae rich in biomass and suitable for use as a biofuel.

Fossil fuel-based industries are now facing a major crisis. This is due to the fact that fossil fuels eventually run out, resulting in supply instability and increased atmospheric CO2 concentrations. Therefore, there is a need for alternative, renewable energy that is carbon neutral, which should be an environmentally and economically sustainable alternative. Among new renewable energy, biodiesel is receiving great attention as a transportation fuel. However, biodiesel, which is generally produced from oil and fat plants, currently replaces only a small portion of transportation fuels, and uses microalgae, a marine biomass that has various advantages as a raw material for biodiesel, to meet the demand for transportation fuels worldwide. It is an alternative that many people are interested in lately. However, in order to put this into practical use, more efforts are needed to increase productivity, and more intensive research is still required.

In the present invention, in order to discover the microalgae rich in biomass content, after collecting the microalgae in the separation from the river water sample, and isolated and identified a new microalgae rich in biomass content and active growth, and analyzed the base sequence As a result of examining the systematic classification, it was confirmed that the microalgae having the same nucleotide sequence did not exist and belonged to the Micractinium inermum systematically .

Therefore, the present inventors deposited a new microalgae isolated and identified in the present invention to the microbial resource center of the Korea Research Institute of Bioscience and Biotechnology on September 24 and was given the deposit number KCTC 12491BP and received the “ Micractinium inermum” . NLP-F014 KCTC 12491BP microalgae ”.

The Micractinium inermum NLP-F014 KCTC 12491BP microalgae isolated and identified in the present invention have higher biomass concentration and total lipid content than other strains, and most of the components in the measured lipids are C16. Fatty acids in the ~ C18 region occupy the effect, which can be used very usefully as a myomass fuel.

Therefore, the present invention can provide a biofuel comprising a novel Micractinium inermum NLP-F014 KCTC 12491BP microalgae or a culture thereof according to the present invention.

The active ingredient of the biomass fuel may include a novel Micractinium inermum NLP-F014 KCTC 12491BP microalgae or a culture thereof, and the culture of the microalgae may include a culture medium including the microalgae. Or, it may include a concentrated cell obtained by centrifugation or filtration to remove the culture medium in the culture and to recover only the concentrated cells, the concentrated cells are frozen or lyophilized according to a conventional method ( lyophilized) to preserve its activity. Preferably, the microalgae and the culture medium of the microalgae may use living microalgae.

Furthermore, the present invention may provide a method of preparing biofuel using Micractinium inermum NLP-F014 microalgae or a culture thereof according to the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. The following examples are merely to illustrate the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited to these examples.

<Example 1>

Microalgae Strains and Culture Conditions

Microalgae Micractinium inermum NLP-F014 KCTC 12491BP isolated from river water samples was used for the discovery of new microalgae capable of high density growth for biodiesel production.

In order to separate the microalgae, the inventors collected a sample sampled along the tributary of the Nakdong River into a 6-well plate, and then, in a plant growth incubator maintained at a temperature of 25 ° C., a humidity of 60% and a brightness of 50 μmol / m ² / s for 5 days It was stored for 10 days. Samples of wells in which microalgal cells were identified in the samples were inoculated on an agar plate. The agar plate was administered to modified Bold's basal medium medium so that the amount of agar and agarose was 2% (w / v), respectively, and the medium was sterilized at 121 ° C. for 15 minutes and then cooled to 60 ° C. Was added to a petri dish (20 mL) and the lid was hardened for 30 minutes, then turned over and dried for one day to completely solidify the medium. Inoculated agar plates were stored until the microalgal colonies were seen in the plant growth incubator. Colonies were isolated and inoculated in 50 mL volume of modified Bold's basal medium and incubated in plant growth incubator for 7 to 15 days. The cultured microalgae culture was used as a seed garden for the following experiment. The following experiments identified Micractinium inermum NLP-F014 KCTC 12491BP capable of high density growth.

In addition, the growth medium was modified Bold's basal medium, the medium was 250 mg / L K ₂ HPO ₄ , MgSO ₄ · 7H ₂ O 251 mg / L, CaCl ₂ · 2H ₂ O 25 mg / L, KH ₂ PO ₄ 250 mg / L, NaCl 25 mg / L, H ₃ BO ₃ 28 mg / L, MnCl ₂ 4H ₂ O 3.60 mg / L, ZnSO ₄ 7H ₂ O 22 mg / L, CuSO ₄ 5H ₂ O 3.92 mg / L, Na ₂ MoO ₄ 2H ₂ O 1.92 × 10 ¹ mg / L, Na ₂ EDTA 2H ₂ O 49.9 mg / L, KOH 31 mg / L, FeSO ₄ 7H ₂ O 12.4 mg / L, H _{2 SO 4 1.86 mg / L, CoCl} 2 · 6H 2 O 7.99 × 10 1 mg / L, NaNO 3 and containing 600 mg / L.

The reactor used for microalgae cultivation is a tubular photoreactor with an internal diameter of 50 mm and a height of 550 mm, with an effective volume of 800 mL. Inside the reactor, a long rod-shaped diffuser was inserted to inject CO ₂ mixed gas (2% CO ₂ + 98% air). Mixed gas was continuously injected at an acid rate of 0.8 L / min, and daylight fluorescent lamps were installed at the front and the rear of the reactor as a light source, and the intensity was 200 μmol / m ² / s. Reactor internal temperature was maintained at 25 ℃, microalgae initial seeding was applied to OD ₇₃₀ 0.2 (about 60 mg / L).

<Example 2>

Genomic DNA Extraction

In order to extract genomic DNA from Micractinium inermum NLP-F014 KCTC 12491BP, we put microalgae in a 1.5 mL tube and centrifuged for 5 minutes at 8000 rpm. The supernatant was removed and only the microalgal pellets were left, followed by extraction solution [(0.8 M LiCl, 0.6% SDS, 10 mM EDTA, 0.2% PVPP; pH 9.0), 0.2 mL β-mercaptoethanol] (Hong et al. 2005) 500 and Proteinase K (Bioneer, Korea) (20 mg / mL) 100 was added. After reacting at 55 ° C. for 10 minutes (mixing every 2-3 minutes), the reaction was performed at 4 ° C. for 1 hour at regular intervals.

The same volume of PCI solution (Phenol: Chloroform: Isoamyl alcohol = 25: 24: 1) (Bioneer, Korea) was added thereto, stirred for 10 minutes, centrifuged at 12,000 rpm for 10 minutes, and the supernatant was transferred to a new tube. Genomic DNA was precipitated by adding 0.1-fold 3 M sodium acetate (pH 5.2) and 2-fold 100% ethanol at 12,000 rpm for 10 minutes. The supernatant was removed, mixed with 700 mL of 70% ethanol, mixed well, centrifuged at 12,000 rpm for 5 minutes, and dried at room temperature for 10 minutes. 50-200 TE buffer (10 mM Tris-Cl, 1 mM EDTA; pH 8.0) and RNase (Promega, USA) (4 mg / mL) 2.5 were added and reacted at 37 ° C. for 30 minutes. The extracted genomic DNA 1 was mixed well with loading dye 1-2 and electrophoresed at 50 V for 60 minutes in 1% agarose. NannoDrop 2000 (Thermo Scientific, USA) was used to confirm the quantity and quality of the extracted genomic DNA. The extracted genomic DNA was stored at 20 ° C. until the next experiment.

<Example 3>

MIClatinum inner ( Micractinium inermum ) Identification of NLP-F014

<3-1> Sequence analysis through PCR analysis

The present inventors conducted the experiment by the following method for molecular biological identification of the Micractinium inermum NLP-F014 KCTC 12491BP strain isolated in the above example.

First, the genomic DNA of the extracted Micractinium inermum NLP-F014 KCTC 12491BP was diluted to 50 ng / 18S rDNA, ITS1, 5.8S rDNA, of ribosomal DNA of microalgae In order to PCR amplify the ITS2 and 28S rDNA regions, 20 doses of AccuPower PCR PreMix (Bioneer, Korea) stored at -20 ° C were used for genomic DNA 1 and PCR primers (5 pmol) each 1 [Euk-A (5'-). AACCTGGTTGATCCTGCCAGT-3 ') (Countway et al. 2005), LSU-D3B (5'-TCGGAAGGAACCAGCTACTA3' (Nunn et al. 1996)) and sterile distilled water 17 were added in turn. A PCR machine (T100 ^™ Thermal Cycler, Bio-Rad, USA) PCR conditions were set as shown in Table 1 below.

After the PCR reaction was completed, electrophoresis was performed on a 1% agarose gel to determine whether the PCR band was present, the amount of PCR product amplified, and the size of the amplified product, which was determined using GelDoc Image System (Bio-Rad, USA). The PCR product was determined using the AccuPrep PCR Purification Kit (Bioneer).

The base sequence of the NLP-F014 strain isolated by PCR method was analyzed, and the base sequence is shown in SEQ ID NO: 1.

<3-2> Generating tree

For analysis of the completed sequencing information, trimming unnecessary nucleotide sequences using BioEdit (Hall 1999) and two fragments of DNA sequences analyzed forward and reverse using BioEdit were made into one full length nucleotide sequence.

The edited sequence was searched by NCBI's BLAST (http://blast.ncbi.nlm.nih.gov/) to confirm that the analyzed sequence information was correct, and the NCBI (http: //www.ncbi.nlm.nih .gov / nucleotide /) downloaded the sequence information of the species to be analyzed and the related species, and sequence alignment was performed using multiple sequence alignment in BioEdit.

As a result, the ribosomal DNA of the NLP-F014 strain of the present invention was found to show a high degree of similarity with Micractinium inermum strain registered in the GenBank.

In addition, the phylogenetic tree was prepared using the neighboring-joining method of MEGA (Molecular Evolutionary Genetics Analysis; ver. 5.1b) (Tamura et al. 2007).

As a result, NLP-F014 of the present invention is a microphone below Tiny moth inner stop (Micractinium inermum) NIES: 2171 and is supported by the high degree of similarity boot's reuraep value (bootstrap value) appears to be a branch with (see Fig. 2).

Therefore, the present inventors named the NLP-F014 strain isolated in the above example as " Micractinium inermum NLP-F014", dated September 24, 2013 to the Korea Research Institute of Bioscience and Biotechnology The deposit was given to the accession number KCTC 12491BP.

<Example 4>

Analysis of Biomass Productivity of New Strains According to the Present Invention

<4-1> Determination of the concentration of biomass produced by the new strain of the present invention

The present inventors used a dry weight method to measure the concentration (g / L) of the biomass produced by the new microalgae NLP-F014 identified in the present invention. The dry cell weight was filtered by suction through a pre-weighed dry 1.2 GF / C and microalgal culture, followed by drying for 4 hours at 105 ℃. After drying, the GF / C margin was weighed, and the microalgal biomass concentration was calculated by measuring the GF / C weight before filtration.

Microalgae Micractinium inermum NLP-F014 KCTC 12491BP isolated from river water samples was cultured under photosynthetic autotrophic conditions, resulting in Micractinium inermum NLP-F014 It can be seen that the logarithmic multiplier is shown from day 1 to day 3 through the induction period and reaches the stationary state from day 6. After 7 days of culture, the biomass concentration of Micractinium inermum NLP-F014 KCTC 12491BP showed 5.36 g / L (see FIG. 3).

<4-2> Determination of Total Nitrogen and Total Phosphorus Concentration by New Bacteria of the Present Invention

Total nitrogen (TN) and total phosphorus (TP) concentrations in the culture were determined by the absorbance method using the Humas kit.

During the 7-day incubation period, the concentration changes of TN and TP used by microalgae Micractinium inermum NLP-F014 KCTC 12491BP showed that TN and TP concentrations were not decreased until the first day. Did. TN concentration was absorbed by microalgae Micractinium inermum NLP-F014 KCTC 12491BP in the second day and began to be used for growth. On the third day, the residual nitrogen concentration was almost constant. Was maintained. The TP concentration was injected at the beginning of the cultivation in excess of 100 mg / L to prevent the TP from becoming a limiting reactant and to serve as a buffer to maintain the pH of the culture in the neutral region. The TP concentration gradually decreased between Day 1 and Day 5, and after Day 5, it was found to show a constant residual concentration (see FIGS. 4 to 5).

Therefore, the novel microalgae Micractinium inermum NLP-F014 KCTC 12491BP showed the characteristics of rapidly absorbing nutrients within the first two days in growth conditions under the photosynthetic autotrophic culture conditions applied in the present invention and utilizing them for growth. It can be seen that the mass concentration can be obtained.

<4-3> Measurement of Lipid Content in Mycobacterium strains According to the Invention

We used a modified direct trans-esterification method to analyze lipid content. Micractinium inermum NLP-F014 KCTC 12491BP was analyzed using gas chromatography (Agilent 7890 GC, USA) to analyze the total fatty acid content. (lipid) Add about 10 mg to a test tube, add 2 mL of chloroform: MeOH (2: 1 v / v), mix for 10 minutes with a vortex mixer, and use heptadecanoic acid as an internal standard. 98%, Sigma-Aldrich, Japan) 1 mL, 1 mL of MeOH and 0.3 mL of sulfuric acid were added, mixed with a vortex mixer for 10 minutes, and allowed to react at room temperature for 10 minutes. Cooled enough. Add 1 mL of distilled water to a sufficiently cooled test tube, mix for 5 minutes with a vortex mixer, and centrifuge in a centrifuge (4,000 rpm, 10min). After filtration with a disposable filter, 1 mL was added to the vial and subjected to gas chromatography analysis. Standards used for FAME analysis were performed using Mix RM3, Mix RM5, GLC50, GLC70 (Supelco, USA).

As a result, the total lipid content of Micractinium inermum NLP-F014 KCTC 12491BP was 40.6%, and it was found that most of the components of the measured lipids occupy fatty acids in the C16 ~ C18 region ( 6). This is similar to the fatty acid composition found in biodiesel components.

<4-4> Determination of Biomass and Lipid Productivity of New Strains According to the Present Invention

Biomass productivity was calculated by dividing the date of sampling time by the biomass concentration at the time of sampling. The specific growth rate was calculated by the following equation using the biomass concentration at the induction phase and the induction phase after the induction phase. Here, m means biomass concentration (g / L), and t means time (day).

Lipid productivity was calculated by multiplying the lipid content expressed in mg fatty acids per g cell by the biomass concentration and dividing the date at the time of sampling.

Biomass and lipid productivity, specific growth rate of microalgae Micractinium inermum NLP-F014 KCTC 12491BP at 7 days of culture are shown in Table 2 below. As a result, the culture 7 in the primary point of microalgae microphone below Tiny moth inner stop (Micractinium inermum) biomass productivity of the NLP-F014 KCTC 12491BP is 0.76 g / L / day, the specific growth rate is 1.19 / day, lipid productivity is 0.31 g / L / day showed a high level of microalgae photosynthetic autotrophic growth (see Table 2).

Accordingly, the present invention of novel strain of microphones below Tiny moth inner stop (Micractinium inermum) NLP-F014 KCTC 12491BP has an effect that can be seen with high biomass concentration, as C16 ~ C18 area biofuels the fatty acid content is very high composition of .

So far I looked at the center of the preferred embodiment for the present invention. Those skilled in the art will appreciate that the present invention can be implemented in a modified form without departing from the essential features of the present invention. Therefore, the disclosed embodiments should be considered in descriptive sense only and not for purposes of limitation. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope will be construed as being included in the present invention.

<120> Novel Micractinium inermum NLP-F014 and use

<211> 2979

<212> DNA

<213> Artificial Sequence

<220>

<223> Micractinium inermum NLP-F014 ribosome DNA sequence

<400> 1

gtataaactg ctttatactg tgaaactgcg aatggctcat taaatcagtt atagtttatt 60

tgatggtacc tactactcgg atacccgtag taaatctaga gctaatacgt gcgtaaatcc 120

cgacttctgg aagggacgta tttattagat aaaaggccga ccgggctctg cccgactcgc 180

ggtgaatcat gataacttca cgaatcgcat ggccttgtgc cggcgatgtt tcattcaaat 240

ttctgcccta tcaactttcg atggtaggat agaggcctac catggtggta acgggtgacg 300

gaggattagg gttcgattcc ggagagggag cctgagaaac ggctaccaca tccaaggaag 360

gcagcaggcg cgcaaattac ccaatcctga cacagggagg tagtgacaat aaataacaat 420

actgggcctt ttcaggtctg gtaattggaa tgagtacaat ctaaacccct taacgaggat 480

caattggagg gcaagtctgg tgccagcagc cgcggtaatt ccagctccaa tagcgtatat 540

ttaagttgct gcagttaaaa agctcgtagt tggatttcgg gtggggcctg ccggtccgcc 600

gtttcggtgt gcactggcag ggcccacctt gttgtcgggg acgggctcct gggcttcact 660

gtccgggact cggagtcgac gctgttactt tgagtaaatt agagtgttca aagcaggcct 720

acgctctgaa tacattagca tggaataaca cgataggact ctggcctatc ctgttggtct 780

gtaggaccgg agtaatgatt aagagggaca gtcgggggca ttcgtatttc attgtcagag 840

gtgaaattct tggatttatg aaagacgaac tactgcgaaa gcatttgcca aggatgtttt 900

cattaatcaa gaacgaaagt tgggggctcg aagacgatta gataccgtcc tagtctcaac 960

cataaacgat gccgactagg gatcggcgga tgtttcttcg atgactccgc cggcacctta 1020

tgagaaatca aagtttttgg gttccggggg gagtatggtc gcaaggctga aacttaaagg 1080

aattgacgga agggcaccac caggcgtgga gcctgcggct taatttgact caacacggga 1140

aaacttacca ggtccagaca tagtgaggat tgacagattg agagctcttt cttgattcta 1200

tgggtggtgg tgcatggccg ttcttagttg gtgggttgcc ttgtcaggtt gattccggta 1260

acgaacgaga cctcagcctg ctaaatagtc acgattggct cgccagtcgg cggacttctt 1320

agagggacta ttggcgacta gccaatggaa gcatgaggca ataacaggtc tgtgatgccc 1380

ttagatgttc tgggccgcac gcgcgctaca ctgatgcatt caacgagctt agccttggcc 1440

gagaggcccg ggtaatcttt gaaactgcat cgtgatgggg atagattatt gcaattatta 1500

atcttcaacg aggaatgcct agtaagcgca agtcatcagc ttgcgttgat tacgtccctg 1560

ccctttgtac acaccgcccg tcgctcctac cgattgggtg tgctggtgaa gtgttcggat 1620

tggcgactgg gggcggtctc cgctctcagc cgccgaaaag ttcattaaac cctcccacct 1680

agaggaagga gaagtcgtaa caaggtttcc gtaggtgaac ctgcggaagg atcattgaat 1740

cgatcgaatc cactctggta accaaccgtc ccctcgccct ggtgcgagcg tcggtcccct 1800

gtctggggtc ttctgaccgc agttcaggtc cggcgggcct tacccccacg ggtgtcccct 1860

cggggccccc tgggctgtag ggtcggtaat tatattcaac tcaacccacc ccaaacctaa 1920

acttaaacta aagctgtcta gtgtgtgcat ctcggtgccc cactctaacc aaagacaact 1980

ctcaacaacg gatatcttgg ctcccgtatc gatgaagaac gcagcgaaat gcgatacgta 2040

gtgtgaattg cagaattccg tgaaccatcg aatctttgaa cgcaaattgc gcccgaggct 2100

tcggccgagg gcatgtctgc ctcagcgtcg gcttacaccc tcgctctccc tctcctttgg 2160

agtggataga acggacctgg ccttcccggc tcctcttcga ttcatcgatg agtccgggtc 2220

ggctgaagtg tagaggcttg agcatggacc ccgtttgcag ggcaatgact tggtaggtag 2280

catcgctaca cagcctgtcg ttgtccgagg ggactttgct ggcggcctag caggaattcg 2340

gggtggttct gccaccccga atgtctcaca ctttcgacct gagctcaggc aagactaccc 2400

gctgaactta agcatatcaa taagcggagg aaaagaaact aactaggatg cccttagtaa 2460

cggcgagcga accgggcaaa gcccaacttg aaaatctcca gcctccggct ggcgaattgt 2520

agtctataga agtgctctct gcctcagtcc ggccccaagt cccctggaaa ggggcgtcag 2580

agagggtgag aaccccgttg ggactggatc ctgaggctcc acgagacgct ttcgaagagt 2640

cgggttgttt gggaatgcag cccaaagcag gtggtaaatc ccatctaagg ctaaatacag 2700

acgggagacc gatagcgaac aagtaccgtg agggaaagat gaaaagaact ttgaaaagag 2760

agttaaaaag tgcttgaaat tgttgagagg gaagtgatta gatcctacgg gtgcgcccag 2820

gcacacgtct gcctaacggt tggctgaatg cgctgggtgc tggtcagcat gggttgaccg 2880

ggcgggacaa accccggggg ttgttacctc ggctatgtcg cctggtcgat cgaggaaaga 2940

atggcgctct ctgagtcctt cgggaactgc gtcatcaag 2979

Claims (5)

1. New Micractinium inermum NLP-F014 KCTC 12491BP microalgae capable of producing biofuels.
2. The method of claim 1,

   The microalgae may produce biomass at a concentration of 4 to 6 g / L, biomass productivity is 0.5 to 1 g / L / day, and lipid productivity is 0.1 to 0.5 g / L / day. Micractinium inermum NLP-F014 KCTC 12491BP Microalgae.
3. The method of claim 1,

   The microalgae are micro-algae microalgae ( Micractinium inermum ) NLP-F014 KCTC 12491BP microalgae, characterized in that the total lipid content based on the weight of the lipid relative to the dry weight of the cell.
4. Claim 1 Micractinium inermum NLP-F014 Accession No .: KCTC 12491BP Bioalgae comprising microalgae or its culture as an active ingredient.
5. Claim 1 Micractinium inermum NLP-F014 Accession No .: KCTC 12491BP Bioalgae using microalgae or a culture thereof.

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