WO2015056997A1

WO2015056997A1 - Apparatus and method for purifying fine particle material

Info

Publication number: WO2015056997A1
Application number: PCT/KR2014/009740
Authority: WO
Inventors: 이희영
Original assignee: 메디칸(주)
Priority date: 2013-10-18
Filing date: 2014-10-16
Publication date: 2015-04-23
Also published as: KR20150045376A; KR101709770B1

Abstract

The present invention provides an apparatus and a method for purifying a fine particle material, the apparatus being an apparatus for increasing the purity of a biochemical material in the form of a plurality of fine particles and comprising: an external container having a predetermined volume; a first member having a through hole through which a complex material including a biochemical material can pass, the first member being formed from a filter material having a plurality of pores of a size smaller than the biochemical material; and a transfer means using positive pressure at one end and/or negative pressure at the other end so that the complex material can pass through the first member, wherein, while the complex material is passing through the first member, gas, liquid, and particles smaller than the particles of the biochemical material can be discharged into the external container by means of gravity and/or pressure gradation.

Description

Apparatus and Purification Method of Particle Particles

The present invention is a purification method for removing the impurity component from the particle-type fraction of the material mixed with liquid and derived from a living body such as fat, blood, etc., while removing impurities from the biological material of the particulate form, minimizing the loss of the biological material The present invention relates to a refining apparatus and a method for purifying small particle materials.

Stem cells have the ability to self-replicate and have the ability to differentiate into two or more cells. Recently, research has been conducted to obtain stem cells from adipose tissue in addition to bone marrow. Adipose-derived stem cells isolated from adipose tissue are multipotent stem cells with the ability to differentiate into various types of cells such as fat cells, muscle cells, chondrocytes, and bone cells.

On the other hand, the biological material collected in the fresh state always contains a liquid. In particular, fragmented adipose tissue obtained by liposuction contains a large amount of free lipids, chemical liquids, blood, etc., and when left for a long time, separation becomes difficult, and therefore, liquid components are degraded during processing into biomaterials. Often it is necessary to remove them early.

In the conventional method of separating and culturing lipo-separated cells, the inhaled or excised fat is crushed again to disintegrate tissue into collagenase, and then centrifuged to precipitate and separate stromal vascular fraction (SVF). In general, methods for culturing cells have been proposed.

On the other hand, the process of collecting materials from living organisms must maintain a sterile sterile environment and adhere to the principles of pollution prevention, which is cumbersome to use existing industrial means during the collection process and at the same time greatly reduce the value of the materials due to the possibility of contamination. There are many.

In the past, this problem was solved by using complicated and expensive expensive equipment such as centrifuge.However, the method of use is complicated and time-consuming, and the biological particles are damaged by the external shock such as centrifugation. There was a problem.

Meanwhile, in WO2005 / 042730, A) obtaining an aspirate by liposuction; B) transferring to a centrifuge to obtain a cell fraction of liposuction; C) obtaining a cell fraction by centrifugation according to specific gravity; D) A method for producing a collagen-free stem cell, which consists of collecting a cell layer having a lower specific gravity than the erythrocyte layer, has been proposed. However, the patent has a problem that the separation process is complicated and the possibility of receiving an external shock through a process called centrifugation increases.

In addition, side effects due to secondary infection or contamination due to the safety issues to the human body, such as the risk of microbial contamination while the cells are exposed to the outside during the acquisition, isolation, extraction of active ingredients, culture, and re-injection of stem cells This may occur, the active ingredient inside the container at the moment of opening the stopper connected to the container can be contaminated by contact with a large amount of air. Therefore, in the conventional clinic, the cost and time for the stability, such as the separation of stem cells in a clean room of class 1000 or more was required. In addition, in the case of removing the separated substances, such as the separation of the layers separated by the eye clinician there was a problem such as loss of the active ingredient.

Therefore, it has been desired to develop a purification device and a purification method for biological materials that can prevent damage by minimizing secondary contamination and minimizing external impact of stem cells.

An object of the present invention to solve the above problems is to provide a purification apparatus and purification method that can be purified while minimizing damage to stem cells.

It is another object of the present invention to provide a purification tool device and a purification method which are simple in separating stem cells and are not impacted from the outside.

In order to achieve the above object, the present invention provides a device for increasing the purity of a plurality of small particle-type biochemical material, the through-hole is formed so that the outer container having a predetermined volume, the composite material containing the biochemical material can pass through, 송 1 member made of a filter material having a plurality of pores formed in a smaller size than the biochemical material, and transport using one or more of positive pressure or other sound pressure of one end to allow the composite material to pass through the first member Means and a particulate matter purifying apparatus configured to allow particles smaller than the biochemical particles, liquids, gases, etc., to flow into the outer container by one or more of gravity, pressure gradation while the composite material penetrates. do.

In addition, the conveying means of the present invention provides a small particle material purification apparatus configured to be introduced by pressing the composite material at a positive pressure at one end of the first member.

In addition, the conveying means of the present invention provides a small particle material purification device configured to suck the composite material into the negative pressure at one end of the first member.

In addition, a liquid is filled between the outer container outer wall of the present invention and the first liquid member to attract impurities, which are liquid components, from the composite material by a diffusion phenomenon in the liquid, and the filling liquid is introduced into the first liquid member. It provides a small particle material purification apparatus characterized in that the composite material is configured to reduce the amount of material having a smaller size than the filter pore or a liquid component.

In addition, a capillary phenomenon-inducing substance having small pores is filled between the outer container outer wall of the present invention and the first-first member, so as to suck only the liquid component from the composite material by a capillary phenomenon. Provided is a small particle material purification device, characterized in that configured to reduce the amount of phosphorus material.

In addition, the outer container of the present invention provides an apparatus for purifying particulate matter, characterized in that n (n ≧ 2) outer containers are sequentially penetrated and connected.

In another aspect, the present invention provides a device for refining small particle material, characterized in that the n outer container is continuously connected to each other so as to penetrate through the n outer container, the first member is connected to one.

In another aspect, the present invention provides a device for refining particulate matter, characterized in that the first member is sealed to the outside by a connecting tube between the outer container and the outer container.

In addition, each of the outer container of the present invention is filled with a liquid having a different material solubility to provide a small particle material purification apparatus, characterized in that different materials are dissolved and diffused in each container.

In addition, the composite material of the present invention provides an apparatus for purifying particulate matter, characterized in that it comprises one or more impurities of enzymes, buffers, and pharmaceutical agents mixed with cells.

In addition, the composite material of the present invention provides a device for refining the small particle material is configured in such a way that the pre-oil outflow to the outer container by applying a positive pressure in the first member as a fatty tissue.

In addition, the composite material of the present invention provides an apparatus for purifying particulate matter, characterized in that the mixture is a mixture of polymer microbeads, surfactant, and distilled water.

In addition, the material accumulated in the through hole of the first member of the present invention provides a device for refining small particle material, characterized in that for pushing by using a pushing means.

In addition, one side of the outer container of the present invention is movable, it provides a small particle material refining apparatus, characterized in that the filling liquid filled in the outer container is injected or discharged through the passageway connected to one side.

In addition, the present invention is a method for increasing the purity of a plurality of small particle-type biochemical material, the through-hole is formed to pass through the outer container having a certain volume, the composite material containing the biochemical material, impurities in the composite material A first means (1) comprising a filter material having a plurality of pores formed in a smaller size than the particles, and a moving means coupling step of coupling the transport means to allow the composite material to pass through the first member; Negative or positive pressure forming step for allowing the composite material to penetrate; A movement step in which particles smaller than the biochemical particles, liquid, gas, and the like move into the outer container by one or more of gravity, pressure gradation; And an obtaining step obtained by allowing the biochemical material to flow out to the other end after the migration.

In another aspect, the present invention provides a method for purifying particulate matter material is configured such that the conveying means is introduced by pressurizing the composite material at a positive pressure at one end of the '1' member.

In another aspect, the present invention provides a method for purifying particulate matter material is configured to suck the composite material into the negative pressure at one end of the '1' member.

In addition, a liquid is filled between the outer container outer wall of the present invention and the first liquid member to attract impurities, which are liquid components, from the composite material by a diffusion phenomenon in the liquid, and the filling liquid is introduced into the first liquid member. The present invention provides a method for purifying particulate matter, characterized in that the composite material has a particle size smaller than that of the filter pore or is configured to reduce the amount of the liquid component material.

In addition, the present invention is filled with a capillary phenomenon-inducing material having small pores between the outer container outer wall and the first 1 member to suck only the liquid component from the composite material by the capillary phenomenon to the liquid component in the composite material It provides a method for purifying small particle material, characterized in that configured to reduce the amount of phosphorus material.

In addition, the outer container of the present invention provides a method for purifying small particle materials, characterized in that n (n≥2) outer containers are sequentially penetrated and connected.

In another aspect, the present invention provides a method for purifying small particle material, characterized in that the n outer container is connected to each other in succession so as to penetrate through the n outer container, the first member is connected to one.

In another aspect, the present invention provides a method for purifying small particle material, characterized in that the tube is sealed between the outer container and the outer container.

In addition, the present invention is characterized in that when the n (n ≥ 2) outer containers are connected in sequence, the filling liquid of each outer container is filled with a liquid with a different material solubility so that different materials are dissolved and spread in each container It provides a method for purifying small particle materials.

In another aspect, the present invention provides a method for purifying small particle material comprising the step of collecting by lyophilizing the material accumulated in the outer container.

In addition, the material accumulated in the through hole of the first member of the present invention provides a method for refining small particle material, characterized in that for pushing by using a pushing means.

Apparatus and a method for purifying small particle materials according to the present invention are related to a method for purifying a biomaterial which can remove impurities from a biological material and minimize the loss of the biological material.

In addition, the apparatus for purifying particulate matter and the purifying method according to the present invention may purify the biomaterial using liquid transfer means other than the pressure difference in order to reduce the phenomenon of adhesion between the filter and the adhesive biomaterial.

In addition, the apparatus for refining small particles and purifying method according to the present invention has an advantage of a simple method and easy use.

In addition, the small particle material purification device and purification method according to the present invention has the effect of minimizing the purification process time, while being easy to use by the biological operator to reduce the device manufacturing cost and maintain a disinfected environment.

1 is a cross-sectional view of a small particle material purification tool according to an embodiment of the present invention.

Figure 2 shows a cross-sectional view of a small particle material purification tool according to another embodiment of the present invention.

Figure 3 shows a cross-sectional view of a small particle material purification tool according to another embodiment of the present invention.

Figure 4 shows a cross-sectional plan view of a small particle material purification tool according to another embodiment of the present invention.

Figure 5 shows a cross-sectional view of a small particle material purification apparatus according to another embodiment of the present invention.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings. First, it should be noted that in the drawings, the same components or parts denote the same reference numerals as much as possible. In describing the present invention, detailed descriptions of related well-known functions or configurations are omitted in order not to obscure the subject matter of the present invention.

As used herein, the terms "about", "substantially", and the like, are used at, or in close proximity to, numerical values as are indicative of the manufacturing and material tolerances inherent in the meanings mentioned, Absolute figures are used to prevent unfair use by unscrupulous infringers.

The present invention provides a device for increasing the purity of a plurality of small-particle-type biochemical material, the outer container having a predetermined volume, and the through-hole is formed to pass through the material is smaller than the material particles, attached through the container A first conveying means made of a filter material having one or more pores of a size, and a first conveying means using one or more of positive pressure or other negative pressure to pass through the composite material containing the material through the first member; During the passage of the composite material, particles smaller than the material particles, liquids, gases, etc. are configured to flow into the vessel by one or more of gravity, pressure gradation.

The present invention invents a method for separating stem cells by applying a liquid transfer means other than a pressure difference in order to reduce the adhesion of the filter and the adhesive material when purified to increase the purity of biochemical materials such as biological material biological results It was.

Previous stem cell separation has been used to increase the purity and concentration, washing and centrifugation, or evaporation, filtering for concentration after washing. In particular, in the stem cell area, which has recently received much attention, it is inevitable to centrifuge to separate stem cells, and to separate a plurality of centrifuges and to add a clean liquid (solvent) for washing foreign substances. This causes a lot of time and stem cell loss that occurs frequently in the process of separating the stem cells. To remedy this problem, a method of automating washing concentrations together using multistage filtering has been introduced and used (Cellution, Cytori ^™ ). However, since the result obtained by separating the stem cells is in the range of about 0.01cc ~ 3cc, if the pores of the filter is slightly large, or if the washing solution is mixed a lot, there is an inconvenience to be concentrated by centrifugation again.

Manual cell-operated stem cell purification, which is now used most of the time, is inevitable after washing, and filtering small particles, such as cells, is intended to discard larger particles trapped in the filter.

If filtering is performed while leaving particles of 10 ~ 20㎛ size like a cell, it can be concentrated due to the pressure difference between the filter and the small substance moves. Since it is not easy to collect them again, it is rare to use a filter for concentration and mainly centrifugation. Therefore, the washing process is concentrated to centrifugation after dilution of the biological material with a washing solution, etc. became an essential process. However, dilution and centrifugation takes a long time, resulting in a drop in viable cell yield, difficult automation control, and inevitable phenomenon of centrifugal damage to cells.

1 is a cross-sectional view of a small particle material purification apparatus according to an embodiment of the present invention, Figure 2 is a cross-sectional view of a small particle material purification apparatus according to another embodiment of the present invention, Figures 3 and 4 Cross-sectional and planar cross-sectional views of an apparatus for purifying particulate matter according to another embodiment of the present invention are also shown.

The present invention consists of an outer container 110 having a predetermined volume and a first member 120 made of a through hole through the outer container, wherein the first member 120 may be a filter. In other words, in order to minimize the loss and damage of the biological material in the particulate state while using a readily available filter, not only the pressure gradient flow but also the diffusion of the liquid (Df) or the capillary phenomenon (Cf) through the pores of the filter. A new method of use and apparatus for use.

Referring to FIG. 1, a through hole is formed in the outer container so that a composite material including a biochemical material may pass therethrough, and the first member 120 of the filter material having a plurality of pores having a smaller size than the biochemical material particles is formed. While the first member 120 moves inside, particles smaller than the pores of the composite material to be washed pass through the pores, and biochemical material particles larger than the pores exit the outer container 110. It is done.

A biochemical material is injected into the first member 120 having a plurality of pores present in the outer container 110 by injecting a composite material containing a biochemical material such as a biomaterial from one side of the through hole. Impurities may be purified while moving out of the first member 120 that is the filter material.

The filter to be purified from the composite material containing biochemicals and impurities has a method in which the liquid is flowed out of the filter by creating a pressure gradient to allow the liquid to flow out of the filter inside the filter having a pore size smaller than the particles. This can be seen as a pressure gradient flow. However, in the biomaterial treatment area, which is the target material to be obtained by the particles caught in the filter, even a small amount of loss should be minimized as much as possible. Since the biomaterial is mostly sticky, the rate of loss of the material attached to the filter is high, and the pressure gradient flow method is limitedly used. .

The composite material containing the biochemical material used in the present invention is a sticky material having a particle shape of 3 μm to 3 mm or less in which an enzyme, a buffer, a drug, and the like are combined with a protein formed of a solid cell, a collagen tissue, and a polysaccharide phospholipid. Can be targeted.

In addition, the composite material may be an adipose tissue containing pre-oil, and may be a mixture of polymer microbeads, surfactant, and distilled water.

Separation using a filter is the easiest and most common method for separating biomaterials without centrifugation. However, when the total amount of particles to be obtained, such as stem cells, is less than about 0.1 cc, the filter is used because the loss due to the pores of the filter or rarely through the filter can cause a relatively large loss. A different method from the pressure gradient flow using the phosphorus pressure difference is applied. That is, by passing through the filter formed long in the shape of the through-hole can be minimized that the micromaterials of the stem cells remaining in the pores of the filter is lost.

The shape of the first member 120 to be used is a tube-shaped through hole that can quickly move the composite material, the shape of the tube wall is preferably composed of a filter formed with fine pores. Unlike other industrial areas, the biomaterial is a filter, such as an o-ring, configured to move between enclosed spaces to minimize the possibility of infection and to prevent loss, unlike other industrial areas. It is preferable that all the materials pass through the inside of the filter using the contact member means.

As a means of meeting these conditions, a piston syringe medical sap bag may be used, and a luer-lock may be used to connect them with the ends of the through holes.

The conveying means may be formed to allow the composite material to pass through the first member 120. The composite material may be conveyed to a conveying means using one or more of a positive pressure or a negative sound pressure.

More specifically, the composite material may be introduced by pressurizing the composite material with positive pressure at one end of the first member 120 or by sucking the composite material with negative pressure at one end of the first member 120. Can be transferred.

Further, in the present invention, in purifying small particle materials such as biochemical materials, the available principle may utilize diffusion or capillary phenomenon. This is a method of utilizing the inherent energy transfer by bringing together materials that are well ordered as homogeneous materials, as in the theory of entropy law. For example, there is a diffusion phenomenon caused by the action of intermolecular attraction, and the other is that when the liquid is in contact with a small and small void, the surface tension with the solid surface is stronger than the surface tension between the liquid, so that the liquid There is a capillary phenomenon that permeates the gas. They are powerful enough to move in the opposite direction of gravity, which can be useful when targeting small particle materials.

Diffusion is a phenomenon in which molecules of higher concentrations are spread to smaller portions when substances having different concentrations are mixed. In the present invention, when the liquid is filled in the outer container 110, the liquid impurities in the composite material These pores lead to diffusion. That is, due to the diffusion phenomenon, particles or liquid components having a smaller size than the filter pores in the composite material are reduced to reduce the amount in the composite material.

In purifying stem cells, in order to purify small particle materials, such as biochemicals containing substances smaller than the pores of the liquid and the filter by diffusion, both the inside of the filter and the outside of the filter are liquid (solvent). It should be included, the concentration of the liquid to be removed should be lower than the concentration in the outer side, which is large enough to the size of the filter pores do not cause the osmosis membrane phenomenon, it is possible to purify the impurities by the diffusion phenomenon.

Meanwhile, in the present invention, a liquid is filled between the outer wall of the outer container 110 and the first liquid member to attract impurities, which are liquid components, from the composite material by a diffusion phenomenon in the liquid, and the filling liquid is introduced into the first liquid member. Inflow can be configured to reduce the amount of material having a smaller size or liquid component than the filter pore in the composite material. The filling liquid is used for washing by drawing in impurities from the composite material.

The capillary phenomenon is a phenomenon in which the surface of the liquid inside the tube becomes higher or lower than the outer surface when a narrow and long tube is inserted into the liquid, which is caused by the cohesion of the liquid and the adhesion between the tube and the liquid. The absorption of nutrients and water can be explained by the capillary phenomenon. In the present invention, the capillary phenomenon may occur when the pores are formed into empty spaces and the composite material, which occupies a large portion of the liquid, is injected into the first member 120. The liquid component of the composite material penetrates into the filter and moves inside the first member 120 while capillary phenomenon occurs.

Accordingly, in the present invention, in purifying a composite material including small particle materials such as biochemical materials, the first member 120 is formed in the outer container 110 to move through the small particle material and the first member is moved. 120 may be made of a filter, which forms pores to allow liquid and small particles of small particle material to move through the pores.

On the other hand, the both ends of the through-hole is formed with a connecting tube 130, the cross section of the connecting tube 130 is larger than the distal end of the injection vessel is injected, the outer periphery of the end of the injection vessel connecting tube 130 ) To match the inner circumference of the By doing so, it is possible to cause diffusion at a rapid rate due to the pressure difference when the object to be washed flows into the through hole.

In addition, the outer container 110 of the present invention may be configured integrally with the through hole. That is, the outer container 110 itself is formed as a filter having a plurality of pores such as the through hole is characterized in that it is formed into the through hole.

In addition, the outer container 110 is preferably directly connected to an injector such as a syringe containing a composite material. In order to prevent the biological material from being contaminated by minimizing exposure to the outside due to the direct connection of an injector such as a syringe to the outer container 110. That is, after directly collecting a biochemical material from a human body, such as a syringe, it is directly connected to the outer container 110 of the present invention and passed through, so that contact with external air is blocked in the process of purification to minimize contamination. do.

In the first member 120, the pore size of the filter is 100nm ~ 10㎛, characterized in that the average diameter is 1 ~ 10mm.

As the pore size of the filter is 100nm ~ 10㎛ to prevent the solid particles of the biological material from going out of the filter. In addition, the diameter of the filter is preferably configured to match the size of the injector, such as a syringe containing a composite containing a biochemical material.

Referring to FIG. 2, it can be seen that the composite material, such as a biochemical material, is washed by capillary action. A filter having a predetermined size having a plurality of pores formed in the outer container 110 is provided. Is composed of a through hole of a predetermined size penetrating the outer container 110, the composite material moves through the through hole of the first bonsai, when the particles smaller than the pores in the composite material is absorbed into the pores by the capillary phenomenon Only the biomaterial particles larger than the pores may be purified by passing through the first member 120.

The first member 120 in the outer container 110 is formed as an empty space, and when purified using only capillary action, the liquid may not be filled in the outer container 110. That is, no fill liquid may be present. When the composite material is injected into the first member 120, a capillary phenomenon may occur. Particles smaller than the liquid and pores of the composite material in the first member 120 penetrate the pores, and the capillary phenomenon occurs. The member 120 may move inside. In more detail, when injected into the first member 120, the molecular force around the first member 120 made of through holes is greater than the surface tension of the liquid in the composite material. The biomaterial particles having a solid property larger than the pores while naturally moving can be moved without moving to the pores in the first member 120 (through hole).

When a predetermined pressure is applied to the injection unit for injecting the cleaning object from the first member 120, adhesion between the particles and the filter is strongly induced by intermolecular attraction, thereby allowing the composite material to be purified through the outer container 110. .

In addition, the purification apparatus of the present invention may be formed with two or more of the outer container 110 in order to make the cleaning more systematic and efficient.

That is, the outer container 110 may be connected to the n (n ≥ 2) outer container 110 is sequentially passed through. In addition, the n outer containers 110 may be continuously connected to each other so as to penetrate through the n outer containers 110, and the first member 120 may be connected to form one.

Referring to FIG. 3, the outer container 110 for cleaning is formed such that three (n = 3) pieces are connected and the first member 120 is formed as one, and the filling liquid in each outer container 110 is To block movement to another outer container 110 and to connect the first member 120 between the outer container 110 and the outer container 110 or to prevent the first member 120 from being exposed to the outside It may be made of a tube (130). The connection pipe 130 may or may not include a first member made of a filter, and may be sealed to the outside. The connecting tube 130 may be a tube for medical instruments.

In addition, when n (n≥2) outer containers 110 are sequentially connected, the filling liquid of each outer container 110 is filled with a liquid having a different material solubility so that different materials melt and diffuse in each container. can do.

As such, when there are a plurality of outer containers 110, a small amount of impurity liquid and pores in the composite material passing through the first member 120 pass through each of the outer containers 110, and a large amount is first washed. Subsequently, washing is performed sequentially, so that the particulate matter can be purified more cleanly.

On the other hand, the material accumulated in the through hole of the first member 120 can be pushed out using a pushing means, and examples of the pushing means are not particularly limited, and any means that can pass through the straight through hole is limited. Can be applied without.

In addition, the present invention may include a process of freeze-drying and collecting the material accumulated in the outer container (110).

Referring to Figure 4, it shows a planar cross-sectional view of the small particle material purification apparatus of the present invention, a plurality of connected to each other can be made in a certain shape and overlapping each other, a plurality of outer container 110 is connected to each other overlapping, When the object to be washed is injected from one side by connecting the tube, it can be washed through a plurality of outer containers 110 can be washed more clean and efficient.

As described above, the apparatus for purifying small particles and purifying method according to the present invention can minimize the loss ratio of the filter type purification of biochemicals including biological particles, reduce the cost of the filter device, and maintain the disinfected environment. Easy to use directly by the operator, there is an effect that can minimize the overall purification treatment time.

Referring to FIG. 5, one side 111 of the outer container 110 may be movable.

One side 111 of the outer container 110 is movable, and the filling liquid filled in the outer container 110 may be injected or discharged through the access pipe 112 connected to the one side 111. .

The one side 111 may function as a piston of the syringe, which is sealed so that the filling liquid does not go out through one side, and moves from one side of the outer container 110 to the outer container 110. The volume within can be varied. The filling liquid may be injected or flowed out through the access pipe 112 connected to the one side 111.

More specifically, when one side is located outside, as shown in (a) of FIG. 5, the volume of the outer container 110 is the largest state. In this case, the filling liquid may be injected to the maximum. Injection of the filling liquid may be performed by using a syringe or the like through the access pipe 112. As shown in (b) of FIG. 5, the filling liquid mixed with impurities may be discharged to the outside by moving the one side 111 inward.

In addition, when the volume of the outer container 110 is changed from the state of FIG. 5 (b) to FIG. 5 (a) without additional injection of the filling liquid, the pressure in the outer container 110 is lowered. Impurities in the material may be more effectively moved out of the first member 120.

The present invention described above is not limited to the above-described embodiment and the accompanying drawings, and various substitutions, modifications, and changes are possible within the scope without departing from the technical spirit of the present invention. It will be evident to those who have knowledge of.

Claims

A device for increasing the purity of biochemicals in the form of small particles,

An external container of constant volume,

Through holes are formed to pass through the composite material containing the biochemical material, the first member is made of a filter material formed of a plurality of pores of a smaller size than the biochemical material;

Transfer means for using at least one of positive pressure at one end or negative pressure at the other end to allow the composite material to pass through the first member;

And small particles, liquids, gases, and the like, which are smaller than the biochemical material particles, are discharged into the outer container by one or more of gravity, pressure gradation while the composite material is penetrating.
The method of claim 1,

The conveying means is a small particle material purification device configured to be introduced by pressing the composite material at a positive pressure at one end of the first member.
The method of claim 1,

The transport means is a small particle material purification device configured to suck the composite material at negative pressure from one end of the first member.
The method according to any one of claims 1 to 3,

The liquid is filled between the outer wall of the outer container and the first member, drawing impurities in the liquid component from the composite material by the diffusion phenomenon in the liquid, and introducing the filling liquid into the first member. A particulate matter purifying apparatus, characterized in that it is configured to reduce the amount of a substance having a particle size smaller than a filter pore or a liquid component.
The method according to any one of claims 1 to 3,

The amount of material that is a liquid component in the composite material is filled by capillary phenomenon causing material having small pores between the outer wall of the outer container and the first member, so as to suck only the liquid component from the composite material by capillary phenomenon. Particle material purification apparatus, characterized in that configured to reduce.
The method according to any one of claims 1 to 3,

The outer container is a small particle material purification apparatus characterized in that the n (n≥2) outer container is sequentially penetrated and connected.
The method according to any one of claims 1 to 3,

and n outer containers are continuously connected to each other so as to penetrate through the n outer containers, and wherein the first members are connected as one.
The method of claim 7, wherein

The small particle material refining apparatus, characterized in that the first member between the outer container and the outer container is sealed to the outside by a connecting tube.
The method of claim 7, wherein

Each outer container is filled with a liquid having a different material solubility so that different materials are dissolved and diffused in each container.
The method according to any one of claims 1 to 3,

The complex material is a small particle material purification device characterized in that it contains at least one impurity of enzymes, buffers, drugs 혼합 mixed with the cells.
The method according to any one of claims 1 to 3,

The composite material is a small-particle material purification apparatus is configured in such a way that the pre-oil outflow to the outer container by applying a positive pressure in the first member as fatty tissue.
The method according to any one of claims 1 to 3,

The composite material is a particulate material purification device, characterized in that the mixture of polymer microbeads and surfactant, distilled water.
The method according to any one of claims 1 to 3,

The material accumulated in the through-holes of the first member is purified by using a pushing means.
The method according to any one of claims 1 to 3,

One side of the outer container is movable, small particle material purification apparatus characterized in that the filling liquid is filled in or out of the outer container through the access pipe connected to one side.
As a method for increasing the purity of biochemicals in the form of many small particles,

A through hole is formed so that the outer container having a predetermined volume and the composite material containing the biochemical material can pass therethrough, and the first member is made of a filter material having a plurality of pores having a smaller size than the impurity particles in the composite material; A moving means coupling step of coupling a transfer means to allow the composite material to pass through the first first member;

Negative or positive pressure forming step for allowing the composite material to penetrate;

A movement step in which particles smaller than the biochemical particles, liquid, gas, and the like move into the outer container by one or more of gravity, pressure gradation; And

A method for purifying particulate matter comprising the obtaining step obtained by allowing the biochemical material to flow out to the other end after migration.
The method of claim 15,

The conveying means is a small particle material purification method configured to flow by pressing the composite material at a positive pressure at one end of the '1' member.
The method of claim 15,

And the conveying means is configured to suck the composite material into negative pressure at one end of the '1' member.
The method according to any one of claims 15 to 17,

The liquid is filled between the outer wall of the outer container and the first member, drawing impurities in the liquid component from the composite material by the diffusion phenomenon in the liquid, and introducing the filling liquid into the first member. A method for purifying particulate matter, characterized in that it is configured to reduce the amount of material having a particle size smaller than that of the filter pore or liquid component.
The method according to any one of claims 15 to 17,

The amount of material that is a liquid component in the composite material is filled by capillary phenomenon causing material having small pores between the outer wall of the outer container and the first member, so as to suck only the liquid component from the composite material by capillary phenomenon. Particle material purification method, characterized in that configured to reduce.
The method according to any one of claims 15 to 17,

The outer container is a small particle material purification method characterized in that the n (n≥2) outer container is sequentially penetrated and connected.
The method according to any one of claims 15 to 17,

n outer containers are continuously connected to each other so as to penetrate through the n outer containers, wherein the first member is connected to one.
The method of claim 21,

A method for purifying small particle materials, characterized in that the connection is sealed between the outer container and the outer container.
The method of claim 21,

When n (n≥2) 2 outer containers are connected in sequence, the filling liquid of each outer container is filled with a liquid having a different material solubility so that different materials are dissolved and diffused in each container. Way.
The method according to any one of claims 15 to 17,

And freeze-drying and collecting the material accumulated in the outer container.
The method according to any one of claims 15 to 17,

The material accumulated in the through-holes of the first member is pushed out by using a pushing means.