WO2015018376A1 - Protéine zmwak présentant une résistance élevée au charbon des inflorescences, gène la codant et son utilisation - Google Patents

Protéine zmwak présentant une résistance élevée au charbon des inflorescences, gène la codant et son utilisation Download PDF

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WO2015018376A1
WO2015018376A1 PCT/CN2014/084125 CN2014084125W WO2015018376A1 WO 2015018376 A1 WO2015018376 A1 WO 2015018376A1 CN 2014084125 W CN2014084125 W CN 2014084125W WO 2015018376 A1 WO2015018376 A1 WO 2015018376A1
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plant
seq
nucleotide sequence
polynucleotide
protein
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PCT/CN2014/084125
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English (en)
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Mingliang Xu
Weiliang ZUO
Guoqing TAN
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China Agricultural University
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

Definitions

  • the present invention relates to gene engineering field. Specifically, it relates to a protein ZmWAK with high resistance to head smut, encoding gene and use thereof.
  • Head smut is a soil borne disease, which severely impairs the production of maize and broomcorn. Head smut was first reported in 1914, and became a major disease of various main maize-producing regions throughout the world. In China, head smut mostly occurs in spring maize-producing regions of North-eastern regions, as well as North-western regions such as, Inner Mongolia and the like. In early 1990s, due to the planting of susceptible varieties, the incidence of head smut increased year by year, resulting in up to about three hundred thousand tons of production loss annually. In 2002, a large-scale outbreak of head smut occurred in maize-producing regions in Northeast China, with the infected area being above one million hm and representing about 20 % of total planting area, resulting in 10-15% of yield loss of maize.
  • Head smut is a fungal disease caused by Sporisorium reilianum.
  • the teliospores on the gall of the infected plant overwinter in soil and become the main source of infection in the coming year.
  • the teliospore of Sphacelotheca reiliana can be viable in soil for 3-5 years, and the spore germination does not need physiological dormancy for maturity.
  • the teliospores in soil having different compatible mating types mate to form a diploid infection hyphae, which in turn infects maize seedling.
  • the optimal temperature for infection of teliospore is 23-30°C under lower or moderate soil moisture content.
  • tassels and ears of diseased plants have also shown abnormal phenotypes, such as teratogeny, being monoecious, ear being leaf-like, and the like. Since head smut eventually destroys the floral organs of maize, it is a disease which causes total loss of production. Once head smut is broken out, it will severely affect the yield.
  • transgenic plant which has an enhanced resistance to head smut, comprising a gene encoding a ZmWAK protein, wherein: said gene encoding said ZmWAK protein is selected from the group consisting of:
  • a polynucleotide comprising a nucleotide sequence of positions 70-2,259 of SEQ ID NO: l of the sequence listing;
  • a polynucleotide comprising a nucleotide sequence encoding the protein of SEQ ID NO:2 of the sequence listing;
  • a polynucleotide comprising a nucleotide sequence of positions 4,001 -10,000 of SEQ ID NO:3 of the sequence listing;
  • a polynucleotide comprising a nucleotide sequence capable of being hybridized with the nucleotide sequence of positions 70-2,259 of SEQ ID NO: l or the nucleotide sequence of positions 4,001 - 10,000 of SEQ ID NO:3 of the sequence listing under high stringent hybridization conditions; and e) A DNA sequence having homology of more than 90%, more than 95%, more than 96%, more than 97%, more than 98%, more than 99% with those defined in a), b), c) or d). That is to say, the disclosure provides a transgenic plant which is transformed with a gene encoding said ZmWAK protein and said ZmWAK protein is expressed in said transgenic plant.
  • the plant is a dicotyledonous or monocotyledonous plant.
  • the monocotyledonous plant is a maize plant.
  • the protein with high resistance to head smut is named as ZmWAK and is derived from maize (Zea mays).
  • the protein comprises an amino acid sequence selected from the group consisting of:
  • amino acid sequence shown in SEQ ID NO:2 of the sequence listing consists of 730 amino acid residues.
  • the ZmWAK protein of 1) or 2) can be artificially synthesized.
  • an encoding gene can be synthesized first, and then the protein can be obtained by biological expression.
  • the protein can also be derived by deletion and/or mutation and/or addition of one or more codons of the DNA sequence of positions 70-2,259 of SEQ ID NO: 1 of the sequence listing.
  • Nucleic acid molecule encoding the ZmWAK protein is within the protection scope of the invention.
  • the nucleic acid molecule can be DNA, such as cDNA, genomic DNA or recombinant DNA; the nucleic acid molecule can also be RNA, such as mRNA, hnRNA or tRNA.
  • Gene encoding the protein is within the protection scope of the invention.
  • the gene encoding the protein comprises one of the following nucleotide sequences: a) A polynucleotide comprising a nucleotide sequence of positions 70-2,259 of SEQ ID NO: 1 of the sequence listing;
  • a polynucleotide comprising a nucleotide sequence encoding the protein of SEQ ID NO: 2 of the sequence listing;
  • a polynucleotide comprising a nucleotide sequence of positions 4,001 -10,000 of SEQ ID NO: 3 of the sequence listing;
  • a polynucleotide comprising a nucleotide sequence capable of being hybridized with the nucleotide sequence of positions 70-2,259 of SEQ ID NO: l or the nucleotide sequence of positions 4,001 -10,000 of SEQ ID NO:3 of the sequence listing under high stringent hybridization conditions; and e) A DNA sequence having homology of more than 90%, more than 95%, more than 96%, more than 97%, more than 98%, more than 99% with those defined in a), b), c) or d).
  • the high stringent hybridization conditions can be hybridization under 65 °C with a solution of 6xSSC, 0.5% SDS, and then the film is washed in 2xSSC, 0.1 % SDS and l SSC, 0.1 % SDS, respectively.
  • SEQ ID NO: l of the sequence listing consists of 2,517 nucleotides with the open reading frame thereof being at nucleotide positions 70-2,259 from 5' end, which codes for the protein shown in SEQ ID NO:2 of the sequence listing, i.e., the ZmWAK protein of the invention.
  • Recombinant vector, expression cassette, transgenic cell line or host bacteria comprising the encoding gene above are also within the protection scope of the invention.
  • the recombinant vector refers to recombinant expression vector or recombinant cloning vector.
  • the recombinant expression vector can be constructed by available expression vector.
  • the recombinant expression vector can also contain a 3 ' untranslated region of an exogenous gene, i.e., the polyadenylation signals, and any other DNA fragments involved in mRNA processing or gene expression.
  • the recombinant expression vector can contain an enhanced promoter, a constitutive promoter, a tissue-specific promoter or an inducible promoter, alone or in combination with other promoters. Additionally, the recombinant expression vector can also contain an enhancer such as a translational enhancer or a transcriptional enhancer.
  • the plant expression vector can also contain one or more genes in order to identify and screen/select for transgenic cells or plants, such as genes capable of generating colour-changeable enzyme or luminous compound upon expression in the plant (GUS gene, GFP gene, fluorescein enzyme gene, and the like), antibiotic resistant selectable marker genes (gentamycin marker, kanamycin marker, and the like), or anti-chemical reagent marker genes (such as anti-herbicide genes).
  • genes capable of generating colour-changeable enzyme or luminous compound upon expression in the plant GUS gene, GFP gene, fluorescein enzyme gene, and the like
  • antibiotic resistant selectable marker genes gentamycin marker, kanamycin marker, and the like
  • anti-chemical reagent marker genes such as anti-herbicide genes.
  • transgenic plants can be screened directly for stress tolerance without the use of any marker gene.
  • the recombinant expression vector is a plasmid obtained by inserting nucleic acid sequence shown in SEQ ID NO: 3 into polycloning sites of starting vector pCAMBIA 1300.
  • Another aspect of the disclosure provides use of the protein, the encoding gene or the recombinant expression vector, expression cassette, transgenic cell line or host bacteria for enhancing resistance to head smut of a plant.
  • the plant is a dicotyledonous or monocotyledonous plant.
  • the monocotyledonous plant is a maize plant.
  • Still another aspect of the disclosure provides use of the protein, the encoding gene or the recombinant vector, expression cassette, transgenic cell line or host bacteria in producing a transgenic plant.
  • the transgenic plant has an enhanced resistance to head smut compared with a control plant that does not comprise the encoding gene, or the recombinant expression vector, or the expression cassette.
  • the plant is a dicotyledonous or monocotyledonous plant; in another embodiment, the monocotyledonous plant is a maize plant or broomcorn plant.
  • Still another aspect of the disclosure provides a method for producing a transgenic plant, comprising introducing the encoding gene or the recombinant vector, or the expression cassette into a target plant; and obtaining the transgenic plant therefrom.
  • the transgenic plant has an enhanced resistance to head smut compared with a control plant that does not comprise the encoding gene, or the recombinant vector, or the expression cassette.
  • the plant is a dicotyledonous or monocotyledonous plant; in another embodiment, the monocotyledonous plant is a maize plant or broomcorn plant.
  • the disclosure provides a progeny of any generation of the transgenic plant of the invention, which comprises a ZmWAK protein or a gene encoding said ZmWAK protein as defined above. Said progenies have resistance to head smut like their parent plant.
  • the disclosure provides a seed of any generation of the transgenic plant of the invention, which comprises a ZmWAK protein or a gene encoding said ZmWAK protein as defined above. Said seed is harvested from a parent transgenic plant or a progeny thereof.
  • Primer pairs used to amplify the full length of the encoding gene or any fragments thereof is within the protection scope of the invention.
  • the primer pairs are selected from the group consisting of:
  • a first primer comprising a polynucleotide having the nucleotide sequence shown in SEQ ID NO:4 of the sequence listing; and a second primer comprising a polynucleotide having the nucleotide sequence shown in SEQ ID NO:5 of the sequence listing;
  • a first primer comprising a polynucleotide having the reverse complementary sequence of SEQ ID NO:4 of the sequence listing; and a second primer primer comprising a polynucleotide having the reverse complementary sequence of SEQ ID NO: 5 of the sequence listing.
  • Another aspect of the disclosure provides a use of the molecular markers in identifying the encoding gene and/or in detecting whether the plant to be detected has resistance to head smut.
  • the plant is a dicotyledonous or monocotyledonous plant; in another embodiment, the monocotyledonous plant is a maize plant or broomcorn plant.
  • FIGURES Fig. l is a graph showing the identification of Zm WAK-t nsge ic maize by a molecular marker genoWAK2.
  • Fig.2 is a graph showing phenotypic comparison between Zm WAK-tmns genie maize and non-transgenic maize.
  • Fig.3 is a graph showing statistical result of disease resistance between Zm i3 ⁇ 44ii-transgenic maize and non-transgenic maize.
  • Maize Hill used in the following Examples can be obtained from China Agricultural University, and was described in Armstrong C, Green C, Phillips R. Development and availability of germplasm with high Type II culture formation response. Maize Genetics Cooperation Newsletter 1991.
  • Gall of diseased plant from the previous year was collected, stored in a cool and dry place away from light, and used as a seeding source in the coming year. After the gall was initially screened using a sieve of 100 mesh to remove the residual host plant vascular tissue, the smut spores were collected for seeding. Inbred Jil 037 with high resistance to head smut was inoculated with Sporisorium reilianum. After 48 h, total RNA of root tissue of the infected Jil 037 was extracted using TriZol reagent provided by Invitrogen Corporation.
  • First chain cDNA was synthesized with BD SMARTTM RACE cDNA Amplification Kit, and the primer sequences for RACE are:
  • WAK-3'RACE 5'- AACTACACCTTCAAGGCATCCGACC -3 '
  • WAK-5'RACE 5'- GACTTCGAACTGGAACCTGATCTCG -3 '
  • the above 5 '- and 3 ' -RACE products were cloned into the pEASY-Tl vector (from Beijing QuanShiJin Biotechnology Co., Ltd), and the positive clone was selected for sequencing and splicing to obtain sequence of the full length cDNA of ZmWAK gene, as shown in SEQ ID NO: l of the sequence listing, which has 2,517 bp, with a coding region of 2, 190 bp, corresponding to nucleotide positions 70-2,259 of SEQ ID NO: l of the sequence listing.
  • the Zm WAK gene encodes a protein of 730 amino acids, with the amino acid sequence shown in SEQ ID NO:2 of the sequence listing.
  • cDNA of Ji l 037 was amplified using the following primers to obtain the full-length cDNA transcript of Zm WAK coding region.
  • flcWAKL 5 '- ATGTCATCACTCCTGTTGCGAG -3 '
  • flcWAKR 5'- ATGTGCCGACCGACCATTC -3 '
  • Nucleic acid fragment amplified by the above primer pair was sequenced to confirm it contained the nucleic acid sequence shown at nucleotide positions 70-2,350 of SEQ ID NO: l of the sequence listing, containing nucleic acid sequence of the Zm WAK gene coding region.
  • a positive clone was selected for sequencing, showing that the sequence of the insert in the vector had the nucleic acid sequence shown in SEQ ID NO: 3 of the sequence listing, which contains the genome sequence of the Zm WAK gene.
  • the vector pCAMBIA1300 with the NO:3 insert at BamEl site was named as pl 300-WAK.
  • the fragment having nucleotide sequence of positions 4,001- 10,000 of SEQ ID NO:3 of the sequence listing was named as ZmWAK.
  • Maize material Hill is the Fi hybrid of parents A and B, and both A and B were confirmed to be sensitive to maize head smut.
  • the recombinant expression vector pl 300-WAK obtained above was transformed into Agrobacterium EHA105.
  • the expression vector was transformed into immature embryos of maize material Hill sensitive to maize head smut using Agrobacterium-mediated method.
  • Transformed callus was selected by hygromycin resistance, and was regenerated to obtain transgenic positive plant.
  • a blank vector was used as a control at the same time.
  • a molecular marker genoWAK2 was used to identify the transgenic progeny.
  • genoWAk2R 5 '- GCGTGAGCCTATCTAGCGAC -3 ' .
  • the nucleic acid sequence of the above genoWAK2F is shown in SEQ ID NO:4 of the sequence listing; and the nucleic acid sequence of the above genoWAK2R is shown in SEQ ID NO:5 of the sequence listing.
  • the primer pair consisting of genoWAK2F and genoWAK2R was designed according to the reverse complementary strand of Zm WAK gene coding strand.
  • the amplification product was loaded on an agarose gel for electrophoresis detection.
  • the detection result showed a band of about 446 bp present in plants of experimental group, and positive control group of expression vector plasmid, while the corresponding DNA fragment was detected in neither ddH 2 0 blank control nor maize material Hill of the negative control group not transfected with the expression vector.
  • Zm WAK gene was incorporated to the genome of maize material Hill of the above experimental group.
  • T 0 transgenic plants identified as positive were selfed to obtain Tj. Positive Ti plants were crossed with Huangzao4 which is highly susceptible to head smut, to obtain T]Fi progeny. T]Fi plants were selfed to obtain T]F 2 . T ⁇ i plants were back-crossed to Huangzao4 to obtain BQTiFi.
  • Fig. 1 Part of the identification results by the molecular marker genoWAK2 is shown in Fig. 1.
  • M represents the 2-kb molecular marker.
  • Lane 1 is expression vector plasmid; lane 2 is genome DNA of parent A; lane 3 is genome DNA of parent B; lane 4 is ddH 2 0 blank control, and lanes 5-16 are # 17-2 transgenic event Tj positive plants.
  • the progeny were divided into two classes according to the genotype identification result:
  • the plant with the detected446-bp band was defined as a progeny with the insertion of Zm WAK-transgene. According to the detection result, this type of plants included: the T]F] positive segregant plants of both #17-2 and #9-21 , the T]F 2 positive segregant plants, and the positive segregant plants of #17-2.
  • the plant without the detected 446 bp band was defined as a progeny without the insertion of Zm WAK-transgemc fragment. According to the detection result, this type of plants included: the TiFi negative segregant plants of both #17-2 and #9-21 , the T]F 2 negative segregant plants, and the negative segregant plants of #17-2.
  • the two classes of progeny were assessed for their phenotypes.
  • the diagnosis for maize head smut is as follows: the maize plant whose tassels and ears contained smut spore or that showed anomalous growth was defined as sensitive to maize head smut, while normal plants were defined as resistant to maize head smut.
  • Statistical analysis was performed to determine whether there was a significant difference in disease response between the above two types of progeny that could be attributed to the function of the gene. A blank vector was used as a control at the same time.
  • Phenotypic evaluation the smut spores from the gall of diseased plant of the previous year were collected, stored in a ventilated place away from light, and used as a seeding source for the following year. Maize seeds were germinated in a tray covered with soil containing ⁇ %o smut spores and seedlings were grown in a tray for one month. Young seedlings were transplanted in the field for continued growth and development. The plants were evaluated for disease resistance during milky maturity period. The evaluation included observation on whether there was typical symptom in tassels and ears of the maize. The bracteal leaf of ear was peeled to observe whether there was smut gall internally when necessary.
  • Fig. 2 shows in the BC]T]Fi segregating progeny of #17-2, plants with the inserted transgene (positive segregants, designated as plant with transgene in Fig. 2) showed normal ear, and extruded silk and pollinated successfully. However, the ear of plants without the inserted transgene (negative segregants, designated as non-transgene in Fig. 2) was replaced by smut spore of pathogen. Silk did not extrude and the plants could not be pollinated normally. The phenotype of the blank vector control was identical to that of plant which is designated as non-transgenic plant in Fig. 2.
  • Fig. 3 The results of statistical analysis on disease resistance are shown in Fig. 3.
  • the results of Fig. 3 show that, the plants with Zm WAK-transg e (positive segregants)from the segregating progeny from the cross between the T] transgenic positive plants of the two transgenic events #17-2 and 9-21 and Huangzao4 (Fig. 3 A, wherein ** means p ⁇ 0.01); as well as the plants with Zm f3 ⁇ 44Jf-transgene from the BQTjF] segregating progeny from the cross between transgenic positive plants of T]Fi of #17-2 and Huangzao4 (Fig.

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Abstract

L'invention concerne une protéine Zm WAK de maïs, le gène la codant et son utilisation. La protéine a l'une des caractéristiques suivantes : 1) une protéine constituée de la séquence d'acides aminés indiquée dans SEQ ID NO: 2；2) une protéine dérivée de la séquence d'acides aminés indiquée dans SEQ ID NO: 2 par substitution et/ou délétion et/ou addition d'un ou plusieurs résidus d'acide aminé, et associée à une résistance au charbon des inflorescences. La protéine et le gène codant la protéine peuvent être utilisés pour produire une plante transgénique présentant une résistance élevée au charbon des inflorescences, par une approche par reproduction et/ou transgénique. La présente invention concerne également les plantes transgéniques ainsi obtenues.
PCT/CN2014/084125 2013-08-09 2014-08-11 Protéine zmwak présentant une résistance élevée au charbon des inflorescences, gène la codant et son utilisation WO2015018376A1 (fr)

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CN201310346549.1A CN104341494B (zh) 2013-08-09 2013-08-09 一种高抗丝黑穗病的蛋白ZmWAK及其编码基因和其应用

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CN109705200A (zh) * 2019-02-26 2019-05-03 中国农业大学 灰斑病抗性相关蛋白ZmWAK-RLK及其编码基因和应用
CN117069816A (zh) * 2023-09-28 2023-11-17 广东省农业科学院作物研究所 一种玉米ZmBAG1基因及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010022328A2 (fr) * 2008-08-21 2010-02-25 E. I. Du Pont De Nemours And Company Locus génétiques associés à la résistance du charbon de l'épi dans le maïs
CN101824479A (zh) * 2010-05-07 2010-09-08 沈阳师范大学 高粱抗丝黑穗病菌3号生理小种的scar标记
CN102533748A (zh) * 2012-02-13 2012-07-04 东北农业大学 与玉米抗丝黑穗病基因连锁的SNP位点、基于该位点的分子标记LSdCAP2及其应用
CN102559668A (zh) * 2012-02-13 2012-07-11 东北农业大学 与玉米抗丝黑穗病基因连锁的SNP位点、基于该位点的分子标记LSdCAP3及其应用

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CN101575644B (zh) * 2009-05-08 2012-02-01 吉林大学 玉米丝黑穗病菌pcr检测试剂盒及其制备方法
CN101773040B (zh) * 2010-02-01 2012-05-30 吉林省农业科学院 一种抗丝黑穗病玉米的辅助育种方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010022328A2 (fr) * 2008-08-21 2010-02-25 E. I. Du Pont De Nemours And Company Locus génétiques associés à la résistance du charbon de l'épi dans le maïs
CN101824479A (zh) * 2010-05-07 2010-09-08 沈阳师范大学 高粱抗丝黑穗病菌3号生理小种的scar标记
CN102533748A (zh) * 2012-02-13 2012-07-04 东北农业大学 与玉米抗丝黑穗病基因连锁的SNP位点、基于该位点的分子标记LSdCAP2及其应用
CN102559668A (zh) * 2012-02-13 2012-07-11 东北农业大学 与玉米抗丝黑穗病基因连锁的SNP位点、基于该位点的分子标记LSdCAP3及其应用

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