WO2015002919A3 - Regulation of production pathways in host cells - Google Patents

Regulation of production pathways in host cells Download PDF

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Publication number
WO2015002919A3
WO2015002919A3 PCT/US2014/044996 US2014044996W WO2015002919A3 WO 2015002919 A3 WO2015002919 A3 WO 2015002919A3 US 2014044996 W US2014044996 W US 2014044996W WO 2015002919 A3 WO2015002919 A3 WO 2015002919A3
Authority
WO
WIPO (PCT)
Prior art keywords
host cells
regulation
production pathways
polypeptides
controlled
Prior art date
Application number
PCT/US2014/044996
Other languages
French (fr)
Other versions
WO2015002919A2 (en
Inventor
Michael Dauner
Mehmedalija Jahic
Daniel P. O'keefe
Jahnavi Chandra PRASAD
Original Assignee
Butamax Advanced Biofuels Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Butamax Advanced Biofuels Llc filed Critical Butamax Advanced Biofuels Llc
Publication of WO2015002919A2 publication Critical patent/WO2015002919A2/en
Publication of WO2015002919A3 publication Critical patent/WO2015002919A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C12P7/26—Ketones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01006—Acetolactate synthase (2.2.1.6)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

The invention relates to recombinant host cells that comprise controlled biocatalyst polypeptides and methods for producing fermentation products employing the same. In some embodiments, various controlled polypeptides and control cues are employed during the propagation and production phases of a fermentation process.
PCT/US2014/044996 2013-07-03 2014-07-01 Regulation of production pathways in host cells WO2015002919A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361842881P 2013-07-03 2013-07-03
US61/842,881 2013-07-03

Publications (2)

Publication Number Publication Date
WO2015002919A2 WO2015002919A2 (en) 2015-01-08
WO2015002919A3 true WO2015002919A3 (en) 2015-06-18

Family

ID=52144268

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2014/044996 WO2015002919A2 (en) 2013-07-03 2014-07-01 Regulation of production pathways in host cells

Country Status (1)

Country Link
WO (1) WO2015002919A2 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100330637A1 (en) * 2008-02-23 2010-12-30 James Weifu Lee Designer Organisms for Photobiological Butanol Production from Carbon Dioxide and Water
US20110312043A1 (en) * 2010-06-18 2011-12-22 Butamax(Tm) Advanced Biofuels Llc Extraction solvents derived from oil for alcohol removal in extractive fermentation
US20120231515A1 (en) * 2008-04-28 2012-09-13 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium achromobacter xylosoxidans
WO2013102147A2 (en) * 2011-12-30 2013-07-04 Butamax (Tm) Advanced Biofuels Llc Genetic switches for butanol production
US20140186911A1 (en) * 2012-12-28 2014-07-03 Butamax Advanced Biofuels Llc Recombinant host cells and methods for producing butanol

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100330637A1 (en) * 2008-02-23 2010-12-30 James Weifu Lee Designer Organisms for Photobiological Butanol Production from Carbon Dioxide and Water
US20120231515A1 (en) * 2008-04-28 2012-09-13 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium achromobacter xylosoxidans
US20110312043A1 (en) * 2010-06-18 2011-12-22 Butamax(Tm) Advanced Biofuels Llc Extraction solvents derived from oil for alcohol removal in extractive fermentation
WO2013102147A2 (en) * 2011-12-30 2013-07-04 Butamax (Tm) Advanced Biofuels Llc Genetic switches for butanol production
US20140186911A1 (en) * 2012-12-28 2014-07-03 Butamax Advanced Biofuels Llc Recombinant host cells and methods for producing butanol

Also Published As

Publication number Publication date
WO2015002919A2 (en) 2015-01-08

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