WO2014184340A1

WO2014184340A1 - Composition of ilex aquifolium extracts and use thereof

Info

Publication number: WO2014184340A1
Application number: PCT/EP2014/060070
Authority: WO
Inventors: Daniel JENNEPIN
Original assignee: Biolis; Jennepin
Priority date: 2013-05-17
Filing date: 2014-05-16
Publication date: 2014-11-20
Also published as: FR3005578B1; FR3005578A1

Abstract

The present invention concerns the use of plant extracts or mixtures of plant extracts, in particular an extract of a plant of the ilex genus, preferably an extract of ilex aquifolium. These plant extracts or mixtures of plant extracts are particularly advantageous for the treatment of conditions, in particular dermatological conditions, or indeed for the properties of same with regard to soothing or cleansing the skin of animals, in particular livestock. The invention also concerns a concentrated composition and a formulation suitable for these uses.

Description

ILEX AQUIFOLIUM EXTRACT COMPOSITION AND USE THEREOF

DESCRIPTION

The present invention relates to the use of plant extracts or mixtures of plant extracts, in particular an extract of a plant of the genus // ex, preferably an extract of // ex aquifolium. These plant extracts or plant extract mixtures are particularly advantageous for the treatment of diseases, especially dermatological conditions, or for their soothing or cleansing properties of the epidermis of animals, especially animals of breeding. The invention also relates to a concentrated composition and a formulation adapted to these uses.

Some plants are known for their particular properties, especially for their vulnerable or resolving qualities, for example for anesthetic properties,

Soothing, healing, disinfecting or emollient.

For example, Sahayaraj et al. have described the use of Pteridium aquilinum extracts obtained by the action of chloroform, for the fight against rust and septoria of peanut plants (Sahayaraj et al., Antifungal activity of three fern extracts on causative agents of groundnut early leaf spot and rust diseases, 2009).

WO-2005/063266 relates to a dermatological composition comprising extracts of Hypericum perforatum and Calendula officinalis. The more specific treatment of psoriasis is aimed at.

In addition, Erdemoglu et al. described the screening of extracts of dry leaves of ex-aquifolium (Erdemoglu et al., Antibacterial, antifungal and antimycobacterial activity of llexacifolium, 2009).

However, these plants are not often used effectively for the treatment of dermatological conditions, particularly because of difficulties in selecting these plants.

Another difficulty relates to the choice of the plants to be associated with each other in order to improve the properties thereof.

Other difficulties relate to methods for preparing plant extracts and their use in concentrated compositions or formulations that can be used directly during application. Other known difficulties in the field of treatments based on plant extracts are the need to go through a dilution step prior to their application, especially on animals or animal breeding sites.

Furthermore, there is a disadvantage or a risk when using extracts of 5 plants which concerns their toxicity which may be prohibitive to their simple and effective use.

Thus, the present invention provides a solution to all or part of these problems.

Other advantages of the invention will become apparent in its detailed description, in particular by the examples of implementations of this invention.

The present invention therefore relates to a concentrated composition comprising at least one extract of a plant of the genus llex in a concentration ranging from 5 to 80 g / l.

15

The present invention also relates to a concentrated composition comprising at least one extract of a plant of the genus llex in a concentration ranging from 5 to 80 g / l, excluding a fruit extract of a plant of the genus llex.

Preferably, the concentrated composition according to the invention comprises at least one extract of fresh leaves or fresh frozen leaves of a plant of the genus llex in a concentration ranging from 5 to 80 g / l.

Advantageously, the plant extract within the composition according to the invention is derived from a plant selected from llex aquifolium, llex aquiliformis, llex guayusa, llex paraguariensis, llex vomitoria, or from a selected plant. among llex aquifolium, llex aquiliformis, llex guayusa, llex vomitoria, preferably it is an extract of Hex aquifolium.

Within the composition according to the invention, the concentration of llex plant extract can range from 15 to 60 g / l or from 25 to 45 g / l. Advantageously, it can be Preferably, the concentrated composition according to the invention also comprises at least one extract of a plant of the genus Pteridium or at least one extract of a plant of the genus Hypericum. More preferably, the concentrated composition according to the invention also comprises at least one extract of a plant of the genus Pteridium and at least one extract of a plant of the genus Hypericum.

Particularly advantageously, within the composition according to the invention, the plant extract of the genus Pteridium is derived from Pteridium aquilifolium, Pteridium aquiliformis or Pteridium aquilinum and the plant extract of the genus Hypericum is derived from Hypericum perforatum or of Hypericum androseanum.

A concentrated composition according to the preferred invention comprises extracts of Hex aquifolium, Pteridium aquilinum and Hypericum perforatum.

Another preferred concentrated composition according to the invention comprises extracts of Hex aquifolium, Pteridium aquilinum and Hypericum androseanum.

Advantageously, the concentrated composition according to the invention comprises

An extract of a plant of the genus ex in a concentration ranging from 5 to 80 g / l or from 15 to 60 g / l or from 25 to 45 g / l or even 30 g / l;

A plant extract of the genus Pteridium in a concentration ranging from 1 to 30 g / l or ranging from 2 to 20 g / l or ranging from 3 to 10 g / l or even 4 g / l and

"A plant extract of the genus Hypericum in a concentration of from 1 to 30 g / l or from 2 to 20 g / l or from 3 to 10 g / l or of 4 g / l.

In a particularly advantageous manner, the concentrated composition according to the invention comprises

An extract of ex aquifolium in a concentration ranging from 25 to 45 g / l;

An extract of Pteridium aquilinum in a concentration of from 3 to 10 g / l;

• an extract of Hypericum perforatum in a concentration ranging from 3 to 10 g / l; or

An extract of ex aquifolium in a concentration ranging from 25 to 45 g / l;

• an extract of Pteridium aquilinum in a concentration ranging from 3 to 10 g / l;

"An extract of Hypericum androseanum in a concentration ranging from 3 to 10 g / l.

The concentrated composition according to the invention may also comprise a support chosen from o thick paraffin oils or paraffin oils having a density at 20 ° C of between 0.860 and 0.890 g / ml and a viscosity at 20 ° C of between 100 mPa.s and 250 mPa.s or oils paraffin wax having a density at 20 ° C of 0.862 to 0.875 g / ml and a viscosity at 20 ° C of 140 mPa.s to 185 mPa.s;

light paraffin oils or paraffin oils whose density at 20 ° C is between 0.810 and 0.855 g / ml and whose viscosity at 20 ° C is between 10 mPa.s and 90 mPa.s or oils paraffin whose density at 20 ° C is between 0.850 and 0.853 g / ml and whose viscosity at 20 ° C is between 25 mPa.s and 35 mPa.s;

the compounds of formula (I)

in which

R represents a group CC ₂₀ alkyl, preferably a C ₈ group - C15 alkyl, in particular a group Ci ₃ alkyl, preferably an n-tridecanyl;

R ² represents a CC pack ₂₀ alkyl, preferably a group Ci - This alkyl, especially a C ₃ alkyl, preferably an isopropyl group;

in particular isopropyl myristate or propan-2-yl tetradecanoate; glycerol or propane-1,2,3-triol;

one or other of these supports and an alcohol chosen from methanol, ethanol, n-propanol, isopropanol, n-butanol and isobutanol, preferably ethanol;

an alcohol chosen from methanol, ethanol, n-propanol, isopropanol, n-butanol and isobutanol, preferably ethanol.

The invention also relates to processes for preparing a concentrated composition according to the invention.

The processes for preparing the concentrated composition according to the invention comprise grinding the leaves of plants used. Preferably, the grinding is carried out with fresh leaves, in particular to make it possible to increase the quantities of substances extracted. However, the preparation process can also be carried out by grinding frozen leaves. Freezing or freezing, especially under conditions limiting or preventing any evaporation of the substances to be extracted, then makes it possible to preserve the leaves between their harvest and the preparation of the concentrated composition according to the invention.

The plants useful for carrying out the processes for preparing the concentrated composition according to the invention are the plants included in this composition.

Thus, the preparation methods according to the invention are implemented from an extract of a plant of the genus Ilex. Advantageously, the plant extract is derived from a plant selected from Ilex aquifolium, Ilex aquiliformis, Ilex guayusa, Ilex paraguariensis, Ilex vomitoria, or is derived from a plant selected from Ilex aquifolium, Ilex aquiliformis, Ilex

Guayusa, Ilex vomitoria, preferably it is an extract of Ilex aquifolium.

Preferably, the processes for preparing a concentrated composition according to the invention also use at least one extract of a plant of the genus Pteridium, in particular Pteridium aquilifolium, Pteridium aquiliformis or Pteridium aquilinum, or at least one extract of a plant of the genus Hypericum, in particular

Hypericum androseanum or Hypericum perforatum.

More preferably, the processes for preparing a concentrated composition according to the invention also use at least one extract of a plant of the genus Pteridium and at least one extract of a plant of the genus Hypericum.

Most preferably, these preparation methods use extracts

Ilex aquifolium, Pteridium aquilinum and Hypericum perforatum or extracts of Ilex aquifolium, Pteridium aquilinum and Hypericum androseanum.

Thus, the invention provides a method (P1) for preparing a concentrated composition comprising

"Grinding fresh leaves or fresh frozen leaves of at least one plant defined for the concentrated composition of the invention;

• the maceration of the ground material in a support selected from paraffin oils or paraffin oils whose density at 20 ° C is between 0.860 and 0.890 g / ml and whose viscosity at 20 ° C is between 100 mPa.s and 250 mPa.s or the oils of paraffin whose density at 20 ° C is between 0.862 and 0.875 g / ml and whose viscosity at 20 ° C is between 140 mPa.s and 185 mPa.s;

light paraffinic oils or paraffin oils having a density at 20 ° C of between 0.810 and 0.855 g / ml and a viscosity at 20 ° C of between 10 mPa.s and 90 mPa.s or oils of paraffin whose density at 20 ° C is between 0.850 and 0.853 g / ml and whose viscosity at 20 ° C is between 25 mPa.s and 35 mPa.s;

the compounds of formula (I)

(L)

in which

an alcohol chosen from methanol, ethanol, n-propanol, isopropanol, n-butanol and isobutanol, preferably ethanol;

• the decantation then the filtration of the product of the maceration.

Advantageously for the process (P1) for preparing a concentrated composition according to the invention, the crushing of the sheets is also carried out in the maceration support.

Also advantageously, this process (P1) according to the invention combines totally or partially the following characteristics:

• the grinding is carried out within three days following the harvest of the leaves or is carried out for a period ranging from 5 to 45 min or is carried out for a period ranging from 10 to 25 min;

• the maceration is carried out for a period ranging from 6 to 36 hours or is carried out for a period of 18 to 30 hours;

• decantation is carried out for a period ranging from 30 minutes to 24 hours or is carried out for a period ranging from 4 to 8 hours.

In a preferred manner, this method (P1) combines the following characteristics:

• the grinding is done within three days after the harvest of the leaves and for a period ranging from 5 to 45 min;

• The maceration is carried out for a period ranging from 6 to 36 hours;

• decantation is carried out for a period ranging from 30 minutes to 24 hours.

More preferably, this method (P1) combines the following characteristics:

• the grinding is carried out within three days following the harvest of the leaves and for a duration ranging from 10 to 25 min;

• the maceration is carried out for a period ranging from 18 to 30 hours;

• decantation is carried out for a period ranging from 4 to 8 hours.

Advantageously, the preparation method (P1) can also combine the grinding of the leaves and the stirring.

The invention also provides a process (P2) for preparing a concentrated composition comprising

Grinding fresh leaves or fresh frozen leaves of at least one plant defined for the concentrated composition according to the invention;

• heating the ground material in an alcohol selected from methanol, ethanol, n-propanol, isopropanol, n-butanol and isobutanol, preferably ethanol;

• centrifugation of the heating product;

• the separation of the supernatant.

Advantageously for the process (P2) for preparing a concentrated composition according to the invention, the grinding of the sheets is also carried out in the alcohol. Also advantageously, this process (P2) according to the invention completely or partially combines the following characteristics: • the grinding is carried out within three days following the harvest of the leaves or is carried out for a period ranging from 5 to 45 min or is carried out for a period ranging from 10 to 25 min;

Heating is carried out at the reflux temperature of the alcohol or is carried out for a period ranging from 5 to 45 min or from 10 to 30 min, for example a duration of 15 min;

Centrifugation of the heating product is carried out for 10 min or 5000 rpm;

• the separated supernatant is conditioned.

In a preferred manner, this process (P2) combines the following characteristics:

Heating is carried out at the reflux temperature of the alcohol and for a period ranging from 5 to 45 min;

Centrifugation of the heating product is carried out for 10 minutes and at 5000 rpm;

• the separated supernatant is conditioned.

More preferably, this process (P2) combines the following characteristics:

Heating is carried out at the reflux temperature of the alcohol and for a period ranging from 10 to 30 min, for example a duration of 15 min;

• the separated supernatant is conditioned.

Advantageously, the process (P2) can also combine the crushing of the sheets with the agitation of the medium.

The present invention also relates to a formulation comprising a concentrated composition according to the invention combined with an oily type support.

The invention also provides a formulation comprising a concentrated composition according to the invention combined with isopropyl myristate (propan-2-yl tetradecanoate) or glycerol (propane-1,2,3-triol). As an oily support, the formulation according to the invention preferably comprises a support chosen from light paraffin oils or paraffin oils whose density at 20 ° C is between 0.845 and 0.855 g / fnl and whose viscosity at 20 ° C is between 10 mPa.s and 90 mPa.s or paraffin oils whose density at 20 ° C is between 0.850 and 0.853 g / ml and whose viscosity at 20 ° C is between 25 mPa. s and 35 mPa.s.

The formulation according to the invention may also comprise a surfactant or emollient or propylene glycol diester of fatty acids of plant origin, saturated and chain lengths of C ₈ -C _0.

Advantageously, the formulation according to the invention comprises from 5 to 30% of concentrated composition according to the invention and from 70 to 95% of support.

Also advantageously, the formulation according to the invention comprises from 5 to 30% of concentrated composition according to the invention and from 70 to 95% of support and an emollient or propylene glycol diester of fatty acids of origin vegetable, saturated and with chain lengths in C ₈ -Ci ₀ .

Particularly advantageously, the formulation according to the invention comprises 10% of concentrated composition according to the invention and 90% of support or 90% of support and an emollient or propylene glycol diester of original fatty acids. vegetable, saturated and with chain lengths in C ₈ -Ci ₀ .

The formulation according to the invention may comprise the different variants of the concentrated composition according to the invention. It may therefore comprise an extract of a plant of the genus Ex or an extract of a plant of the genus ex, excluding a fruit extract. Advantageously, the plant extract is derived from a plant selected from ex aquifolium, ex aquiliformis, ex guayusa, ex paraguariensis, ex vomitoria, preferably it is an extract of ex aquifolium.

Preferably, the formulation according to the invention also comprises at least one extract of a plant of the genus Pteridium, in particular of Pteridium aquilifolium, Pteridium aquiliformis or Pteridium aquilinum, or at least one extract of a plant of the genus Hypericum, in particular Hypericum androseanum or Hypericum perforatum. More preferably, the formulation according to the invention also comprises at least one extract of a plant of the genus Pteridium and at least one extract of a plant of the genus Hypericum.

More preferably, the formulation according to the invention comprises extracts of Ilex aquifolium, Pteridium aquilinum and Hypericum perforatum.

Also preferably, the formulation according to the invention comprises extracts of Ilex aquifolium, Pteridium aquilinum and Hypericum androseanum.

Within the formulation according to the invention, the respective proportions of concentrated composition and support can vary quite widely. However, very good results can be obtained during the implementation of the formulation according to the invention for which the mass ratio composition / support ranges from 2/98 to 15/85 or ranges from 5/95 to 12/88. or is equal to 10/90.

The invention also relates to a process for preparing a formulation according to the invention comprising

The preparation of a concentrated composition according to the invention, in particular according to the process (P1) or according to the process (P2);

The dilution of the concentrated composition in a support chosen from light paraffin oils or paraffin oils whose density at 20 ° C. is between 0.845 and 0.855 g / ml and whose viscosity at 20 ° C. is between 10 ° C. and 10 ° C. mPa.s and 90 mPa.s or paraffin oils whose density at 20 ° C is between 0.850 and 0.853 g / ml and whose viscosity at 20 ° C is between 25 mPa.s and 35 mPa.s; isopropyl myristate or propan-2-yl tetradecanoate; glycerol or propane-1,2,3-triol.

When carrying out the process for preparing a formulation according to the invention, the composition / support mass ratio is generally from 2/98 to 15/85 or preferably from 5/95 to 12/88 or is equal to at 10/90.

The concentrated composition and the formulation according to the invention can be used for the treatment of numerous conditions, in particular dermatological conditions, or for their soothing or cleansing properties of the epidermis of animals, including farm animals.

The composition and the formulation according to the invention can also be used to improve the skin's suppleness, to calm the irritation, to promote healing, to eliminate the dander and to accelerate the regrowth of the hair, in particular of farm animals.

5

Thus, the invention also relates to a concentrated composition according to the invention or a formulation according to the invention for its use to fight against mycosis or human or animal dermatoses or to fight against fungal infections or dermatoses, in particular mycozoonoses, cattle, equines, sheep, goats, pigs, poultry, rodents, insectivores, birds, rabbits, dogs, cats or to control Trichophyton including Trichophyton equinum, Trichophyton erinacei, Trichophyton Trichophyton mentagrophytes, Trichophyton verrucosum, Trichophyton ochraceum, Trichophyton quinckeanum Trichophyton rubrum; Microsporum including Microsporum audouini, Microsporum

Canis, Microsporum equinum, Microsporum gypseum, Microsporum nanum, Microsporum persicolor; Dermatitis including Dermatitis verrucosa with Cladosporium or Phialophora; dermatophilosis with Dermatophilus congolensis; or to soothe or clean the epidermis of animals, especially farm animals; to improve skin suppleness, soothe irritation, promote healing, eliminate scales and accelerate

20 regrowth of the hair, especially farm animals.

The invention also relates to a method of treatment for combating mycosis or human or animal dermatoses or for combating fungal infections or dermatoses, in particular mycozoonoses, cattle, equines, sheep, sheep and goats.

Goats, pigs, poultry, rodents, insectivores, birds, rabbits, dogs, cats or to control Trichophyton including Trichophyton equinum, Trichophyton erinacei, Trichophyton gallinae, Trichophyton mentagrophytes, Trichophyton verrucosum, Trichophyton ochraceum, Trichophyton quinckeanum Trichophyton rubrum; Microsporum including Microsporum audouini, Microsporum

Canis, Microsporum equinum, Microsporum gypseum, Microsporum nanum, Microsporum persicolor; Dermatitis including Dermatitis verrucosa with Cladosporium or Phialophora; dermatophilosis with Dermatophilus congolensis; to soothe or cleanse the epidermis of animals, especially farm animals; to improve flexibility cutaneous irritation, promote healing, eliminate scales and accelerate regrowth of hair, especially livestock; comprising the application of a concentrated composition according to the invention or the application of a formulation according to the invention.

In a particularly advantageous manner, the method according to the invention comprises the application made by spraying a formulation according to the invention or by the spray application of a concentrated composition according to the invention.

The various aspects of the invention will now be described by particular embodiments by means of the following examples.

Example 1 Composition (CD according to the invention

It is a composition comprising plant extracts obtained from fresh leaves of Ilex aquifolium, Pteridium aquilinum and Hypericum androseanum set

15 maceration in oil: 15 grams of fresh leaves of Hex aquifolium, 2 grams of fresh leaves of Pteridium aquilinum and 2 grams of fresh leaves of Hypericum androseanum are ground for 15 minutes in 600ml of paraffin oil (Cooper of Exxon Mobil, density at 20 ° C: 0.865 g / ml and viscosity at 20 ° C: 176 mPa.s). After maceration for 24 hours and decantation for 3 hours, the supernatant is filtered.

500 ml of a concentrated composition (C1) according to the invention are obtained.

Example 2 Composition (C2) According to the Invention

This is a composition comprising plant extracts obtained from fresh leaves of Ilex aquifolium, Pteridium aquilinum and Hypericum androseanum:

25 grams of fresh leaves of Ilex aquifolium, 2 grams of fresh leaves of Pteridium aquilinum and 2 grams of fresh leaves of Hypericum androseanum are milled for 15 minutes. 100 ml of ethanol are added to this mill. The mixture is refluxed for one hour at 70 ° C. and then centrifuged for 10 minutes at 5000 rpm. Centrifugation is repeated under the same conditions. The supernatant is transferred to

A 200 ml flask is made up with ethanol (qs).

200 ml of a concentrated composition (C2) according to the invention are obtained.

Example 3 Composition (C3) According to the Invention It is a composition comprising plant extracts obtained from fresh leaves of Ilex aquifolium, Pteridium aquilinum and Hypericum androseanum macerated in oil and ethanol: 15 grams of leaves fresh Hex aquifolium, 2 grams of fresh leaves of Pteridium aquilinum and 2 grams of fresh leaves of Hypericum androseanum are milled for 15 minutes in 600ml of paraffin oil (Cooper of Exxon Mobil, density at 20 ° C: Q865g / ml and viscosity at 20 ° C: 176 mPa.s) to which 3% by weight of ethanol is added. After maceration for 24 hours and decantation for 3 hours, the supernatant is filtered.

500 ml of a concentrated composition (C3) according to the invention are obtained.

Example 4 Composition (C4) According to the Invention

It is a composition comprising plant extracts obtained from fresh leaves of Hex aquifolium. 15 g of leaves are crushed for 15 min in 600 ml of paraffin oil (density at 20 ° C: 0.865 g / ml and viscosity at 20 ° C: 176 mPa.s) then macerated for 24 hours before settling during 3 hours. The supernatant is then filtered and makes it possible to obtain 500 ml of concentrated composition (C4) according to the invention.

Example 5 Composition (C5) According to the Invention

It is a composition comprising plant extracts obtained from fresh leaves of Hex aquifolium: 15 grams of fresh leaves of Hex aquifolium, are milled for 15 minutes. 100 ml of ethanol are added to this mill. The mixture is refluxed for one hour at 70 ° C. and then centrifuged for 10 minutes at 5000 rpm. Centrifugation is repeated under the same conditions. The supernatant is transferred to a 200 ml flask and made up with ethanol (qs).

200 ml of a concentrated composition (C5) according to the invention are obtained

Example 6 Composition (C6) According to the Invention

It is a composition comprising plant extracts obtained from fresh leaves of Hex aquifolium macerated in oil and ethanol: 15 grams of fresh leaves of Hex aquifolium are milled for 15 minutes. minutes in 600 ml of paraffin oil (Cooper Exxon Mobil, density at 20 ° C: Q865g / ml and viscosity at 20 ° C: 176 mPa.s) to which is added 3% by weight of ethanol. After maceration for 24 hours and decantation for 3 hours, the supernatant is filtered. 500 ml of a concentrated composition (C6) according to the invention are obtained. Example 7 Composition (C7) According to the Invention

It is a composition comprising plant extracts obtained from fresh leaves of Ilex aquifolium, Pteridium aquilinum and Hypericum androseanum macerated in an ester: 15 grams of fresh leaves of Ilex aquifolium, 2 grams of fresh leaves of Pteridium aquilinum and 2 grams of fresh leaves of Hypericum androseanum are milled for 15 minutes in 600ml of isopropyl myristate (Product Aroma Zone, France). After maceration for 24 hours and decantation for 3 hours, the supernatant is filtered.

500 ml of a concentrated composition (C7) according to the invention are obtained.

Example 8 Composition (C8) According to the Invention

This is a composition comprising plant extracts obtained from fresh leaves of Ilex aquifolium, Pteridium aquilinum and Hypericum androseanum macerated in an ester and ethanol: 15 grams of fresh leaves of Ilex aquifolium, 2 grams of fresh leaves of Pteridium aquilinum and 2 grams of fresh leaves of Hypericum androseanum are milled for 15 minutes in 600ml of isopropyl myristate (Product Aroma Zone, France), to which is added 3% by weight ethanol. After maceration for 24 hours and decantation for 3 hours, the supernatant is filtered.

500 ml of a concentrated composition (C8) according to the invention are obtained.

Example 9 Composition (C9) According to the Invention

This is a composition comprising plant extracts obtained from fresh leaves of Ilex aquifolium. 15 g of leaves are crushed for 15 min in 600 ml of isopropyl myristate (Product Aroma Zone, France) then macerated for 24 hours before settling for 3 hours. The supernatant is then filtered and makes it possible to obtain 500 ml of concentrated composition (C9) according to the invention.

30

Example 10 Composition (C10) According to the Invention

It is a composition comprising plant extracts obtained from fresh leaves of Ilex aquifolium macerated in an ester and ethanol: 15 grams of Fresh leaves of Hex aquifolium are milled for 15 minutes in 600 ml of isopropyl myristate (Product Aroma Zone, France) to which 3% by weight of ethanol is added. After maceration for 24 hours and decantation for 3 hours, the supernatant is filtered.

500 ml of a concentrated composition (C6) according to the invention are obtained.

Example 1 1: Formulation (F1) According to the Invention

The concentrated composition obtained in Example 1 (500 ml) is diluted in light paraffin oil (Mosselman oleochemicals, density at 20 ° C: 0.852 g / ml and viscosity at 20 ° C: 29 mPa.s ) until you obtain 5 liters of formula (F1) ready for use.

Example 12: Formulation (F2) According to the Invention

The concentrated composition obtained in Example 2 (200 ml) is diluted in light paraffin oil (Mosselman oleochemicals, density at 20 ° C: 0.852 g / ml and viscosity at 20 ° C: 29 mPa.s) until you obtain 5 liters of formulation (F2) ready for use.

Example 13 Formulation (F3) According to the Invention

The concentrated composition obtained in Example 3 (500 ml) is diluted in light paraffin oil (Mosselman oleochemicals, density at 20 ° C: 0.852 g / ml and viscosity at 20 ° C: 29 mPa.s) until you obtain 5 liters of formulation (F3) ready for use.

EXAMPLE 14 Formulation (F4) According to the Invention

The concentrated composition obtained in Example 5 (200 ml) is diluted in light paraffin oil (Mosselman oleochemicals, density at 20 ° C: 0.852 g / ml and viscosity at 20 ° C: 29 mPa.s) until you obtain 5 liters of formulation (F4) ready for use. An emulsifying agent is added until a homogeneous and stable mixture is obtained.

Example 15 Formulation (F5) According to the Invention

The concentrated composition obtained in Example 6 (500 ml) is diluted in light paraffin oil (Mosselman oleochemicals, density at 20 ° C: 0.852 g / ml and viscosity at 20 ° C: 29 mPa.s) until you obtain 5 liters of formulation (F5) ready for use.

Example 16: Formulation (F6) According to the Invention Of the concentrated composition obtained in Example 1 (500 ml) and 250 ml of Mygliol 840 (propylene glycol diester of vegetable fatty acids, saturated and with chain lengths C ₈ -Ci ₀ whose viscosity at 20 ° C is between 9 and 12 mPa.s) are diluted in light paraffin oil (density at 20 ° C: 0.852g / ml and viscosity at 20 ° C: 29 mPa.s) to obtain 5 liters formulation formulation (F6) ready for use.

Example 17 Formulation (F7) According to the Invention

The concentrated composition obtained in Example 4 (500 ml) is diluted in paraffin oil (density at 20 ° C: 0.865 g / ml and viscosity at 20 ° C: 176 mPa.s) to obtain 5 liters of formulation (F7) ready for use.

Example 18: Formulation (F8) According to the Invention

The concentrated composition obtained in Example 9 (500 ml) is diluted in light paraffin oil (Mosselman oleochemicals, density at 20 ° C: 0.852 g / ml and viscosity at 20 ° C: 29 mPa.s) to obtain 5 liters of formulation (F8) ready for use.

Example 19 Effectiveness Against Ringworm in Cattle

This is a preliminary efficacy test of formulation (F7) of Example 17 for the treatment of Holstein breeding cows.

The cows have round-shaped plates characteristic of ringworm (Trichophyon verrucosum).

The ringlets are covered with the formulation. The treatment is renewed three days after the first application.

The formulation eliminates all the ringworm plates.

Example 20: Efficacy against ringworm affecting a person

This is a preliminary efficacy test of formulation (F7) of Example 17 for the treatment of a pet groomer in frequent contact with dogs and cats.

The groomer presents on the forearm round-shaped plates characteristic of ringworm (Microsporum canis).

The ringlets are covered with the formulation. The treatment is renewed three days after the first application. The formulation causes no skin reaction and eliminates all ringworm. No plaque is visible one week after the start of treatment.

Example 21 Effectiveness Against Ringworm and Horse Mange Mite

This is another preliminary test of the effectiveness of formulation (F7) of Example 17 for the treatment of stable horses (En Gautier in Montbrun-Lauragais, France).

The five treated horses have problems with mud mange (Dermatitis verrucosa with Cladosporium or Phialophora, dermatophilosis with Dermatophilus congolensis) and ringworm (Trichophyton equinus).

The pasterns of the horses affected by the mud scab are treated and the ring-worm plates are covered with the formulation according to the invention. The treatment is renewed three days after the first application. Horses have no skin reaction. As a result of the treatment, the traces of mud mange and ringworm have completely disappeared. These results were obtained in 10 days after the start of

Treatment.

The formulation thus makes it possible to rapidly eliminate all the traces of mud mange as well as the ring-worm plates.

Example 22: Effectiveness against Trichophyton verrucosum on cattle

20 This is an experiment involving 30 young cattle of Montbeliarde, Holstein or cross-breed Charollais / Montbeliarde breeds with moths (Trichophyton verrucosum).

A first batch of 10 cattle was treated with a formulation (F1) according to Example 1 1, a second batch of 10 cattle was treated with a formulation (F2) according to Example 12 and a batch of 10 cattle was treated with a veterinary reference product. This veterinary reference product for the treatment of Trichophyton verrucosum comprises enilconazole (Imaveral commercial product) and has been used according to the recommended conditions.

Protocol:

The treatments were carried out by spraying the animals (on the lesions and on an area of 3 cm around the lesions).

Formulations (F1) and (F2) were applied twice 4 days apart.

The reference product containing enilconazole was applied 4 times at 4 days intervals respecting the recommended dilution rates. Results:

The follow-up of this trial was carried out by an independent veterinarian in charge of monitoring the breeding. She gave an individual rating of each animal according to 3 criteria: alopecia, crusts and scales on days 1, 5, 9, 13, 22 and 30.

A score of 0 corresponds to an absence of a visual sign of impairment, a score of 1 corresponds to a weak impairment, a score of 2 corresponds to a moderate impairment and a score of 3 corresponds to a severe impairment (alopecia). The score awarded corresponds to an overall score of the three criteria.

The results obtained after these evaluations were collated in Table 1 in the form of mean scores.

Conclusion:

All treated animals were cured. Healing was faster for the batches treated following two applications of formulations (F1) or (F2) according to the invention. Comparatively, the batch of cattle treated with 4 applications of enilconazole did not show better results.

Thus, no significant difference was found over the entire duration of the trial between the 2 batches of cattle treated according to the method of the invention and the reference veterinary treatment.

In addition, no problem of intolerance was noted for the treatments using the formulations according to the invention.

Finally, it was noted the superiority of the formulations according to the invention as to their more practical use: time and number of applications reduced by 50% for both formulations according to the invention implemented, ready-to-use product rather than a reference product requiring dilution prior to application, better binding to the lesions of the formulations according to the invention.

Claims

1. Concentrated composition comprising at least one extract of a plant of the genus //ex in a concentration ranging from 5 to 80 g/l.

2. Composition according to claim 1 comprising at least one extract of a plant of the genus //ex in a concentration ranging from 5 to 80 g/l, excluding an extract of fruits of a plant of the genus / /ex.

3. Composition according to claims 1 and 2 comprising at least one extract of fresh leaves or fresh frozen leaves of a plant of the genus //ex in a concentration ranging from 5 to 80 g/l.

4. Composition according to claims 1 to 3 for which the plant is chosen from //ex aquifolium, //ex aquiliformis, //ex guayusa, //ex paraguariensis, //ex vomitoria or for which the plant extract is present in a concentration ranging from 15 to 60 g/l or from 25 to 45 g/l or is 30 g/l.

5. Composition according to claims 1 to 4 also comprising at least one extract of a plant of the genus Pteridium or at least one extract of a plant of the genus Hypericum or also comprising at least one extract of a plant of the genus Pteridium and least one extract of a plant of the genus Hypericum.

6. Composition according to claims 1 to 5 also comprising at least one extract of a plant chosen from Pteridium aquilifolium, Pteridium aquiliformis, Pteridium aquilinum, or at least one extract of a plant chosen from Hypericum androseanum, Hypericum perforatum.

7. Composition according to claims 1 to 6 comprising

• an extract of //ex aquifolium in a concentration ranging from 25 to 45 g/l;

• an extract of Pteridium aquilinum in a concentration ranging from 3 to 10 g/l;

• an extract of Hypericum perforatum in a concentration ranging from 3 to 10 g/l;

or including • an extract of Ilex aquifolium in a concentration ranging from 25 to 45 g/l;

• an extract of Hypericum androseanum in a concentration ranging from 3 to 10 g/l.

8. Composition according to claims 1 to 7 also comprising a support chosen from

• thick paraffin oils or paraffin oils whose density at 20°C is between 0.860 and 0.890 g/ml and whose viscosity at 20°C is between 100 mPa.s and 250 mPa.s or oils paraffin whose density at 20°C is between 0.862 and 0.875 g/ml and whose viscosity at 20°C is between 140 mPa.s and 185 mPa.s;

• light paraffin oils or paraffin oils whose density at 20°C is between 0.810 and 0.855 g/ml and whose viscosity at 20°C is between 10 mPa.s and 90 mPa.s or oils paraffin whose density at 20°C is between 0.850 and 0.853 g/ml and whose viscosity at 20°C is between 25 mPa.s and 35 mPa.s;

• the compounds of formula (I)

in which

R represents a CC ₂₀ alkyl group, preferably a C ₈ -Ci ₅ alkyl group, in particular a Ci ₃ alkyl group, preferably an n-tridecanyl group;

R ² represents a CC ₂₀ alkyl group, preferably a CC ₆ alkyl group, in particular a C ₃ alkyl group, preferably an isopropyl group;

in particular isopropyl myristate or propan-2-yl tetradecanoate;

• glycerol or propane-1,2,3-triol;

• one or other of these supports and an alcohol chosen from methanol, ethanol, n-propanol, isopropanol, n-butanol and isobutanol, preferably ethanol;

• an alcohol chosen from methanol, ethanol, n-propanol, isopropanol, n-butanol and isobutanol, preferably ethanol.

9. Formulation comprising a composition according to claims 1 to 8 and a support chosen from light paraffin oils or paraffin oils whose density at 20°C is between 0.845 and 0.855 g/ml and whose viscosity at 20°C C is between 10 mPa.s and 90 mPa.s or paraffin oils whose density at 20°C is between 0.850 and 0.853 g/ml and whose viscosity at 20°C is between 25 mPa.s and 35 mPa.s; isopropyl myristate or propan-2-yl tetradecanoate; glycerol or propane-1,2,3-triol. î o

10. Formulation according to claim 9 comprising from 5 to 30% of composition according to claims 1 to 8 and from 70 to 95% of support or from 70 to 95% of support and an emollient agent or propylene glycol diester of fatty acids of plant origin, saturated and with C ₈ -Ci ₀ chain lengths.

15 1 1 . Process (P1) for preparing a concentrated composition comprising

• crushing, for example within three days following harvest or for a period ranging from 5 to 45 min or for a period ranging from 10 to 25 min, of fresh leaves or fresh frozen leaves of at least one plant defined according to claims 1 to 8;

20 "maceration of the ground material, for example for a period ranging from 6 to 36 hours or for a period ranging from 18 to 30 hours, in a support chosen from o thick paraffin oils or paraffin oils whose density at

20°C is between 0.860 and 0.890 g/ml and whose viscosity at 20°C is between 100 mPa.s and 250 mPa.s or paraffin oils whose density at 20°C is between 0.862 and 0.875 g/fml and whose viscosity at

20°C is between 140 mPa.s and 185 mPa.s;

o light paraffin oils or paraffin oils whose density at 20°C is between 0.810 and 0.855 g/ml and whose viscosity at 20°C is between 10 mPa.s and 90 mPa.s or oils paraffin whose density at 20°C is between 0.850 and 0.853 g/ml and whose viscosity at 20°C is between 25 mPa.s and 35 mPa.s;

o the compounds of formula (I)

in which

R ¹ represents a CC ₂₀ alkyl group, preferably a C ₈ - C15 alkyl group, in particular a Ci ₃ alkyl group, preferably an n-tridecanyl group;

R ² represents a CC ₂₀ alkyl group, preferably a Ci - Ce alkyl group, in particular a C ₃ alkyl group, preferably an isopropyl group;

in particular isopropyl myristate or propan-2-yl tetradecanoate; o glycerol or propane-1,2,3-triol;

o one or other of these supports and an alcohol chosen from methanol, ethanol, n-propanol, isopropanol, n-butanol and isobutanol, preferably ethanol;

o an alcohol chosen from methanol, ethanol, n-propanol, isopropanol, n-butanol and isobutanol, preferably ethanol;

• decantation, for example for a period ranging from 30 min to 24 hours or for a period ranging from 4 to 8 hours;

• then filtration of the maceration product.

12. Process (P2) for preparing a concentrated composition comprising

• grinding fresh leaves or fresh frozen leaves of at least one plant defined according to claims 1 to 8;

• heating the ground material in an alcohol chosen from methanol, ethanol, n-propanol, isopropanol, n-butanol and isobutanol, preferably ethanol;

• centrifugation of the heating product;

• separation of the supernatant.