WO2014115924A1 - Module de photobioréacteur de type panneau plat et système de culture photobiologique l'utilisant - Google Patents

Module de photobioréacteur de type panneau plat et système de culture photobiologique l'utilisant Download PDF

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Publication number
WO2014115924A1
WO2014115924A1 PCT/KR2013/001451 KR2013001451W WO2014115924A1 WO 2014115924 A1 WO2014115924 A1 WO 2014115924A1 KR 2013001451 W KR2013001451 W KR 2013001451W WO 2014115924 A1 WO2014115924 A1 WO 2014115924A1
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Prior art keywords
culture
plate
supply pipe
light
gas supply
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PCT/KR2013/001451
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English (en)
Korean (ko)
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정상화
박종락
안동규
김종태
김광호
Original Assignee
조선대학교산학협력단
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Publication of WO2014115924A1 publication Critical patent/WO2014115924A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/02—Tissue, human, animal or plant cell, or virus culture apparatus with means providing suspensions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor

Definitions

  • the present invention relates to a flat panel bioreactor module and a photobiological culture system using the same, and more particularly, to a flat biofilm and an improved light irradiation structure for irradiating light to the photobiological culture vessel. It relates to a reactor module and a photobiological culture system using the same.
  • Microalgae can play a role in the treatment of wastewater, immobilization of carbon dioxide, etc., due to their various capabilities, and have been used for the production of useful substances such as fuels, cosmetics, feed, food coloring and pharmaceutical raw materials. In the meantime, useful and high value added substances are constantly being discovered and expanding their applications.
  • a device for culturing photosynthetic microalgae for the purpose of immobilizing carbon dioxide can be largely divided into a mass culture (open system) and a photobioreactor (closed system) outdoors.
  • Outdoor mass cultivation apparatus including pond type has been mainly used in the form of reaction facilities such as lakes or large ponds and is commercially available in some countries.
  • this type of cultivation facility has the advantages of low initial investment and easy maintenance, but it is difficult to contaminate, isolate and purify, low cell concentration, high mass (especially nitrogen source), high water quality and quantity demand, and irregular climate. Due to problems such as conditions, expensive labor costs, the installation is extremely limited. In particular, the effective growth of light is not achieved inside the culture apparatus, the growth rate of the cells is low, the growth yield of the cells is low, and a large installation space is required to remove a large amount of carbon dioxide.
  • the light applied while the microalgae is incubated becomes more and more bulky as the microalgae grows, so that the microalgae on the surface of the reactor can continue to receive light, but the microalgae inside the reactor are microalgae on the surface. Because of the shadow effect will not be able to receive enough light to grow.
  • most of the microalgae photobioreactors designed to date do not overcome this point, and thus the production efficiency thereof is lower than that of other microbial bioreactors. To overcome this and to transmit light efficiently.
  • photobioreactors using internal light sources have been studied. Widely used photobioreactors include tubular photoreactors and vertical columnar photobioreactors that use sunlight as an external light source.
  • the reactor has a structure in which the narrow and long rectangular or cylindrical pipes are densely adhered to circulate the culture in order to maximize the irradiation area exposed to sunlight and to shorten the light transmission distance into the culture.
  • Such photobioreactors have advantages and disadvantages in their respective forms.
  • the reactor using the fiber as the internal light source has a good light efficiency, but there is a problem that the cell adheres to the optical fiber surface.
  • an artificial light source such as a fluorescent lamp
  • Korean Patent No. 0933741 discloses a photobioreactor for culturing microalgae.
  • the published reactor uses LEDs and flexible LEDs and has a structure in which the light source is directly in contact with the culture solution.
  • micro algae are attached to the surface of the light source, thereby reducing light efficiency.
  • Republic of Korea Patent Publication No. 2009-0055170 discloses a cylindrical photobioreactor
  • Republic of Korea Patent No. 0897019 discloses a photobiological reactor for high efficiency microalgae culture.
  • This reactor has a structure in which sunlight is irradiated to the photobioreactor using a reflective collector and an optical fiber.
  • US Patent Application No. 2009/0211150 discloses a technical configuration for producing biomass and biodiesel by culturing microalgae gloella at high concentration using a tubular photobioreactor, and US Patent Application No. 2005/0255584.
  • a tubular photobioreactor having a surface area increased while using a partition of a transparent material is disclosed.
  • U.S. Patent No. 595876 discloses a tubular photobioreactor using a complex parabolic concentrator to improve the light collection efficiency of sunlight.
  • a bioreactor system for culturing published photosynthetic organisms includes a container having an outer surface and an inner surface, an inner surface defining an isolated space configured to hold a plurality of photosynthetic organisms and culture medium, and one or more contained in the isolated space of the container. Equipped with a lighting system that includes a light emitting substrate.
  • the present invention is to solve the above problems, and the planar photobioreactor module that can prevent the light irradiated from the light source to be transmitted to the photobiotic organisms by irradiating the surface light source from the outside of the photobiological culture vessel and
  • the purpose is to provide a photobiological culture system using the same.
  • Another object of the present invention is to provide a flat-type photobioreactor module and a photobiological culture system using the same, which enables continuous culture by maintaining the culture environment of the photobiotic organism in an optimal state.
  • Another object of the present invention is to increase the degree of freedom of design of the flat-type photobiological culture vessel and the light for irradiating the light, and to maximize the light irradiation efficiency and the uniformity of light distribution, the flat photo-organism can increase the productivity of the photobioorganisms
  • the present invention provides a reactor module and a photobiological culture system using the same.
  • the plate-type photobioreactor module of the present invention for achieving the above object is provided with a culture space is coupled to each other to be cultured by injecting the photobioorganism therein, the first surface is provided with support ribs at regular intervals on the outer surface; 2 culture panel bodies, a gas supply pipe installed to penetrate the culture space formed by the first and second culture panel bodies in the transverse direction to discharge the gas introduced therein, and inside the first and second culture panel bodies.
  • a flat type photobiological culture vessel extending transversely to the wall and including at least one vortex forming partition wall formed in an arc shape so as to cause vortices with respect to the ascending air flow of the gas supplied through the gas supply pipe;
  • It is characterized in that it is provided on at least one side of the plate-shaped photobiological culture vessel provided with a surface light source unit for irradiating light for cultivation of the photobioorganism.
  • the culture panel body constituting the plate-shaped photobiological culture vessel is made of a synthetic resin and the support ribs formed in the culture panel bodies narrow the gap from the top to the bottom, and by reflecting the external light in multiple It is possible to increase the amount of light inflow of the plurality of horizontal high rigidity portion corresponding to the pressure of the culture medium, and consists of a vertical high rigidity portion formed in the same cross-section with the horizontal high rigidity portion.
  • the horizontal high rigidity part and the vertical high rigidity part are integrally formed with the culture panel body so that the cross-section of the culture panel body has one of a semi-circular, trapezoidal or polygonal shape.
  • the gas supply pipe has a plurality of discharge ports whose surface is expanded at a set pressure or more.
  • the culture panel main body is formed by fusion of the edges or coupled to both sides of the main body of the rectangular frame shape to form a culture space.
  • the vortex forming partition wall extends in the transverse direction from the inner wall of the main body at a position spaced upwardly from the gas supply pipe, the first lower end of the position that can be in close contact with the second main panel body is located on the front surface
  • the first vortex is formed in an arc shape having a shorter distance from the gas supply pipe than the first upper end facing the main panel main body, and spaced apart from the inner surface of the first main panel main body in which the first upper end is located in front.
  • a second main panel body extending laterally from an inner wall of the main body at a position spaced upwardly from the gas supply pipe, and having a second lower end of a position where the first main panel body can be in close contact with the first main panel body;
  • the second upper end is formed in an arc shape having a shorter distance from the gas supply pipe than the second upper end, and the second upper end is provided with a second vortex forming partition spaced apart from the inner surface of the second main panel main body located at the rear.
  • the first and second vortex forming partitions are provided with at least one independent vortex generating wing to cause vortices in a predetermined direction.
  • a plate inlet tube for injecting a medium for culturing the microalgae of the microalgae into the inside of the culture space in the plate fluorescent bio-culture vessel, and the upper side of the plate-type photobiological culture vessel.
  • a microalgae discharge tube for discharging the photobioorganism cultured from the inside of the culture space is installed, the gas discharge hole for discharging the gas rising in the culture space to the outside is formed on the upper end side of the flat fluorescent culture culture vessel.
  • the planar light source unit for irradiating light onto the flat panel optical biological culture container includes a light guide plate and a lamp module installed at an edge of the light guide plate, and the light emitted from the lamp module is provided on the rear surface of the light guide plate.
  • a light reflection pattern is formed to uniformly irradiate the reaction vessel.
  • the optical organism culture system of the present invention for achieving the above object is provided with a culture space is coupled to each other to be cultured by injecting the photobioorganism therein, the outer surface is provided with a support rib at a predetermined interval, the first, Two culture panel bodies, a gas supply pipe installed to penetrate the culture space formed by the first and second culture panel bodies in a transverse direction and discharging the gas introduced therein, and inside the first and second culture panel bodies
  • a flat type photobiological culture vessel extending transversely to the wall and including at least one vortex forming partition wall formed in an arc shape so as to cause vortices with respect to the ascending air flow of the gas supplied through the gas supply pipe;
  • a plate-type photobioreactor module installed on at least one side of the plate-type photobiological culture vessel and having a surface light source unit for irradiating light for culturing the photobioorganism;
  • the plate fluorescent bio-culture vessel is connected to a medium supply pipe having a lower side connected to a medium supplying pipe equipped with a pump for injecting microalgae into the culture space, and a medium supply tank for storing the medium, and connected to an upper end side of the plate fluorescent bio-culture vessel.
  • An optical biomass storage tank for storing the optical bioorganism cultured in connection with the microalgae discharge tube, a pH measuring sensor for measuring the pH value of the microalgal culture medium, which is installed and cultured in the flat optical culture vessel, oxygen and Dissolved amount measuring sensor for measuring the dissolved amount of carbon dioxide, biomass concentration measuring sensor, illuminance measuring sensor for measuring the illuminance of the light irradiated from the surface light source unit to the plate-type photobioreactor module,
  • control unit for controlling the continuous production of optical bio-organic on the basis of the signal detected from the pump, illuminance measuring sensor, PH measuring sensor, dissolved volume measuring sensor, biomass concentration measuring sensor.
  • the plate-type photobiological culture vessel further comprises a temperature measuring sensor for measuring the temperature of the cultured photobiological culture.
  • the plate type photobioreactor module and the photobiological culture system using the same can prevent the plate type photobiological reaction vessel from being deformed by the pressure of the culture solution stored therein during the culture of the photobiological organism, and the surface light source. Since light can be irradiated from the outside to the inside by the unit, the heat generated from the surface light source unit can be prevented from being transferred directly to the photobioreactor, thereby preventing necrosis of the photo organism.
  • the plate-type photobioreactor module of the present invention and the photobiological culture system using the same are capable of continual production of photobioorganisms by optimizing the cultivation conditions of the photobiotic organism and further improving the productivity.
  • FIG. 1 is a perspective view showing a plate-type photobioreactor module and a photobiological culture system using the same according to the present invention
  • FIG. 2 is an exploded perspective view of the flat photoreactor module shown in FIG. 1;
  • FIG. 3 is a cross-sectional view of the plate-type photobiological reaction vessel shown in FIG.
  • FIG. 4 is a view showing another embodiment of a flat panel photobioreactor module according to the present invention.
  • FIG. 5 is a cross-sectional view of the flat plate type photobiological culture vessel shown in FIG. 4;
  • FIG. 6 is a perspective view showing a surface light source device according to the present invention.
  • 7, 10, 11, 12 are plan views conceptually showing the surface light source device
  • FIG 8 and 9 are views showing another embodiment of the photobiological culture system.
  • the photobiological culture system using the plate-type photobioreactor module according to the present invention is capable of continuously culturing photobioorganisms, and an embodiment thereof is illustrated in FIGS. 1 to 4.
  • the plate-type photobioreactor module and the photobiological culture system 10 using the same is a plate-type photobiological culture vessel having a culture space that can be cultured by injecting photobiological organisms therein (20) ),
  • a gas supply pipe (71) installed at the lower side of the culture space of the plate-type photobiological culture vessel 20, and at least one side of the plate-type photobiological culture vessel (20) (20) includes a flat type photobioreactor module including a surface light source unit 80 for irradiating light for culturing photobioorganisms. It is provided with a gas supply unit 70 for supplying oxygen and carbon dioxide to the culture space in which the photobiotic organisms cultured through the gas supply pipe 71 is stored.
  • a medium inlet tube 92 having a pump 91 for injecting microalgae and / or a medium into the lower side of the plate-type photobiological culture vessel 20 and the inside of the culture space, and incubating the microalgae.
  • Culture medium supply unit (90) comprising a medium storage tank (93) for storing a medium for storing the light and the cultured in connection with the microalgae discharge pipe (101) connected to the upper end side of the flat optical culture vessel (20)
  • the optical bioorganic storage tank 100 for storing the biological organisms is installed in the optical bioorganic storage unit 110 and the flat optical culture vessel, and the PH for measuring the pH value of the cultured microalgal culture liquid Measuring sensor 120, dissolved amount measuring sensor 130 for measuring the dissolved amount of oxygen and carbon dioxide, and for measuring the illuminance of the light irradiated to the planar photobioreactor module from the surface light source unit 80 Illuminance measuring sensor 140, Control to control the continuous production of the photobioorganic gas based on the signal detected from the ion mass concentration sensor 145, the pump, roughness sensor, PH measurement sensor, dissolved volume measurement sensor, biomass concentration measurement sensor
  • the unit 150 is provided.
  • the plate-type photobiological culture vessel 20 constituting the plate-type photobioreactor module 10 is cultured by being coupled to each other so that the photobioorganism is injected and cultured therein as shown in FIGS. 1 and 3.
  • First and second cultured panel bodies 22 and 23 forming 21 are provided, and the first and second cultured panel bodies 22 and 23 are made of a transparent material, glass or synthetic resin material.
  • the first and second culture panel bodies 22 and 23 are coupled to both sides of the main body 24 having a lattice shape (square frame) by the fastening member 25 to form a culture space 21.
  • the first and second culture panel bodies 22 and 23 are supported by support ribs 30 supported on the main body 24 to be installed on or in contact with the surface.
  • the support ribs 30 are supported at both ends by brackets 31 provided at mutually opposite sides of the main body 24.
  • the support rib is composed of a plate-like member (32).
  • the support rib 30 made of a plate-shaped member is installed to be perpendicular to the surface of the first or second culture panel bodies 22 and 23.
  • the support ribs 30 are the first and second culture panel main body 22, 23 and the main body 24 by the water pressure generated by the culture medium in the culture space of the plate-shaped photobiological culture vessel 20 Deformation is prevented.
  • the vortex forming partition 40 increases the dissolved capacity of oxygen or carbon dioxide supplied from the gas supply pipe 71 and circulates the culture solution. Is formed.
  • the vortex forming partition 40 is formed with a first vortex forming partition 41 extending laterally from an inner wall of the main body 24 at a position spaced upwardly from the gas supply pipe 71.
  • Vortex forming partition 41 is the gas supply pipe than the first upper end (41b) of the first lower end 41a of the position where the first culture panel body can be in close contact with the second culture panel body located on the front side
  • a spaced distance from 71 is formed in a short arc shape.
  • a second vortex forming partition 42 is formed on an upper side of the first vortex forming partition 41, wherein the second vortex forming partition 42 is spaced upwardly from the gas supply pipe 71.
  • a second lower end 42a extending laterally from the inner wall of the upper surface 24 and in close contact with the second main panel main body of the front surface is opposed to the second upper end 42b facing the first culture panel main body.
  • the separation distance from the gas supply pipe 71 is formed in a short arc shape.
  • At least one independent vortex generating wing 43 is formed in the first and second vortex forming partitions 41 and 42 to cause vortices in a predetermined direction.
  • the first and second vortex forming partitions 41 and 42 may be provided in plural and spaced apart from the gas supply pipe 71 by a predetermined interval above the culture space.
  • the first and second vortex forming partitions are not limited to the above-described embodiments, and a plurality of guide vanes (wings) are installed at predetermined intervals on the inner surface of the first and second culture panel bodies 22 and 23 forming a culture space. It may be made of).
  • the plate fluorescent biological reaction container 50 is made by combining the first and second case members 51, 55 vacuum-molded by a synthetic resin material.
  • the first case member 51 constituting the plate-type photobiological reaction vessel 50 is formed by vacuum injection molding, and is perpendicular to the first plane portion 52 and the edge of the first plane portion 52, respectively. And a first skirt portion 53 bent in a direction and a first connecting portion 54 extending radially from the skirt portion 53.
  • the second case member 55 like the first case member 51, is perpendicular to the second flat portion 56 corresponding to the first flat portion 52 and an edge of the second flat portion 56.
  • the second skirt portion 57 bent in a direction and a second connecting portion 58 extending radially from the second skirt portion 57 and engaged with the first connecting portion 54.
  • the first connection portion 54 and the second connection portion 58 are ultrasonically fused or vibration-fused. Bonding of the first and second connectors 54 and 58 is not limited to the above-described embodiment, and may be coupled by a coupling member such as a bolt, a nut, and a clip in a state of bonding or packing.
  • the first and second flat portions 52 and 56 of the first and second case members 51 and 55 are formed with horizontal high rigidity portions 61 which gradually become narrower from the upper end side to the lower end side.
  • the horizontal high rigidity portion 61 may increase the amount of light flowing into the inside by multi-reflecting the external light, and can improve the structural strength of the first and second case members 51 and 55.
  • the planar portions 52 and 56 may be formed parallel to each other in a direction perpendicular to the longitudinal direction, or may be formed in an inclined direction in consideration of an irradiation area or structural strength of light.
  • the horizontal high rigidity portion 61 may be formed by combining a horizontal direction and an inclined direction in the first and second flat portions 52 and 56 or a combination of the horizontal direction and the curved direction.
  • the pressure acting on the first and second case members 51 and 55 by the load of the culture solution filled therein increases from the upper side to the lower side. Accordingly, the spacing of the horizontal high rigidity portion 61 is preferably formed closer to the lower side.
  • the first and second case members 51 and 55 may have a vertically rigid portion 66 formed in the longitudinal direction of the first and second planar portions 52 and 56.
  • the vertical high rigidity portion 62 is formed to intersect the horizontal high rigidity portion 61, but is not limited thereto.
  • the horizontal high rigidity portion 61 and the vertical high rigidity portion 62 respectively formed on the first and second flat portions 52 and 56 of the first and second case members 51 and 55 are external light introduced from the outside.
  • the first and second flat portions 52 and 56 may have a cross-sectional shape in one of semi-circular, trapezoidal, or polygonal shapes so as to multi-reflect light emitted from sunlight or a lamp. It is formed by drawing in from the surfaces of the two flat portions 52 and 56.
  • a lens portion having a negative power or a lens portion having a positive power is formed on the bottom and both sides of the horizontal high rigidity portion 61 and the vertical high rigidity portion 62 having the first and second flat portions 52 and 56 introduced therein. Can be.
  • the inner surface of the first and second flat parts 52, 56, the plurality of vortex generators 63 (64) on the inner surfaces facing each other so as to interfere with the flow of the culture medium in which the microalgae are cultured. ) Are formed, protruding from the inner surface of the first and second cases to the inside of the culture space and formed alternately.
  • the surface light source unit 80 is installed on both sides of the flat photobiological reaction vessel 20 for irradiating light to the flat photobiological reaction vessel 20
  • a light guide plate 81 made of a transparent plate-like member and lamp modules 85 as light sources are installed at edges of the light guide plate 81 to irradiate light regardless of the weather.
  • a diffusion pattern 82 is formed on at least one side of the light guide plate 81 to scatter or reflect the optical fiber or the light emitted from the lamp module 85 to the front side.
  • the diffusion pattern 82 may be formed of a reflective pattern formed on the rear surface of the light guide plate 81 using ink or may be formed on the rear surface of the light guide plate 81 by laser beam or mechanical processing.
  • the diffusion pattern 82 may be formed by forming a groove (eg, a V-shaped groove) on a rear surface of the light guide plate 81 in a predetermined pattern.
  • the groove forming pattern may include a scroll shape, a grid shape, a shape of an overlapping polygon, and horizontal or vertical grooves having different pitches.
  • the diffusion pattern 82 formed on the light guide plate 81 is formed in a lattice form from an edge formed in a lattice shape and a central portion of the light guide plate 81 to an edge, that is, an edge at which the lamp module 85 is installed.
  • the light guide plate 81 may be sparsely formed from the center in the horizontal and vertical directions toward the most up and down direction and both sides.
  • a reflector plate 89 may be installed on the rear surface of the light guide plate 81 so as to reflect the light irradiated from the optical fiber and the lamp module 85 onto the rear surface of the light guide plate 81, and the front surface of the light guide plate 81.
  • a diffusion plate (not shown) may be attached.
  • the light guide plate may be made of transparent synthetic resin, glass, quartz, and the like.
  • the lamp module 85 is installed on the edge of the light guide plate 81 is installed on both sides of the light guide plate 81 as shown in Figure 6, the first lamp module (installed on the side of the light guide plate 81 ( 86 and a second lamp module 87 installed at an edge or a lower surface thereof.
  • the first and second lamp modules 86 and 87 have substantially the same structure, and each of the circuit boards 86a and 87a having the same width as that of the side or bottom surface of the light guide plate 81, and Light emitting diodes 86b and 87b are provided on the circuit boards 86a and 87a at predetermined intervals to irradiate light from the edge of the light guide plate 81.
  • inlet grooves 81a are formed in the side and bottom surfaces of the light guide plate 81 corresponding to the light emitting diodes 86b and 87b, so that the light emitting diodes 86b and 87b are inserted into the inlet grooves 82a. It is preferable to prevent the light from being scattered around the edge of the light guide plate, and it is preferable to form irregularities for scattering the light on the inner surface of the drawing groove 82a.
  • the lamp module for irradiating light to the light guide plate 81 is not limited to the above-described embodiment and may use cold cathode fluorescent lamps (CCFL).
  • CCFL cold cathode fluorescent lamps
  • a cold cathode fluorescent lamp in close contact with the edge of the light guide plate 81 and a reflective member may be provided to surround the edge of the light guide plate to prevent light emitted from the cold cathode fluorescent lamp from being irradiated to a region other than the light guide plate.
  • the surface light source unit is not limited to the above-described embodiment, and a plurality of light emitting diodes may be installed in the flat member.
  • the surface light source unit collects sunlight using the condenser lens 160 and then irradiates the edge of the light guide plate 81 to irradiate the sunlight into a flat optical culture vessel. .
  • the condensed light is irradiated to the edge of the light guide plate 81 using the optical fiber 161. Sunlight can be irradiated to the plate-type photobiological culture vessel 20 through.
  • the irradiation of sunlight irradiates light onto the flat type photobiological culture vessel 20 using sunlight on a sunny day, and is installed at the edge of the light guide plate 81 on a cloudy day or at night. Irradiate light using the lamp module.
  • an end portion of the optical fiber connected to the condenser lens may be installed at the edge of the light guide plate, and lamp modules may be installed at both side and bottom edges of the light guide plate, as shown in FIG. 11.
  • the lamp module may be installed at both edges of the lamp module, and the optical fiber connected to the condenser lens may be installed at the lower edge of the lamp module.
  • the reflective pattern may form a densely formed upper edge corresponding to the center portion in the vertical direction and the portion connected to the optical fiber.
  • an optical fiber and a lamp module for irradiating the light collected by the collecting lens may be installed at the edge of each light guide plate to irradiate light onto the flat optical culture vessel.
  • the gas supply unit 70 for supplying oxygen or carbon dioxide to the gas supply pipe 71 is connected to a connection pipe 72 in which a valve is installed at the gas supply pipe 71, and connected to the connection pipe 72.
  • the carbon dioxide tank 75 and the oxygen tank 76 are connected by the branch pipes 73 and 74.
  • the branch pipes 73 and 74 are provided with valves 77 and 78 for controlling the supply of gas.
  • the gas supply pipe 71 is made of a flexible and elastic tube, the discharge hole 71a is formed in the gas supply pipe 71 at predetermined intervals.
  • the discharge hole 71a is formed so that the tube can be opened while expanding when the pressure in the gas supply pipe 71 becomes equal to or higher than the set pressure.
  • the discharge hole formed in the gas supply pipe is preferably formed in 0.01 to 0.05mm to be normally blocked by the elastic force of the gas supply pipe (71).
  • the medium supply pipe 92 of the medium supply unit 90 may be provided with a valve for intermittent supply of the medium, the culture of the photobioorganic discharge pipe 101 constituting the optical bioorganic storage unit 110 is cultured
  • a control valve 103 may be installed to control the discharge of the completed photobioorganic gas.
  • control unit 150 enables continuous production of the photobioorganism, and each sensor, that is, the PH measuring sensor 120 and the dissolved amount measuring sensor 130 for measuring the dissolved amount of oxygen and carbon dioxide. And a system for controlling the system based on a signal of an illuminance measuring sensor 140 and a biomass concentration measuring sensor 145 for measuring illuminance of light irradiated from the surface light source unit 70 to the flat panel bioreactor module. Equipped with a microcomputer.
  • the biomass concentration measurement sensor is Opt is preferably used ASD19-N, the dissolved oxygen sensor is preferably used Mettler toledo M300.
  • control unit may be further provided with a temperature sensor 151 for measuring the temperature of the photo organisms cultured by the plate-type photobiological culture vessel.
  • the plate-type photobioreactor module and the photobiological culture system using the same according to the present invention configured as described above are capable of culturing continuous photobioorganisms.
  • a medium including a microalgae for culturing the photobioorganisms is supplied to the culture space 21 of the plate-type photobioreactor 20 through the medium supply unit 90.
  • the planar photobiological reaction vessel 20 is irradiated with light using the surface light source unit 80, and the medium for culturing stored in the storage space 21 using the gas supply unit 70. Feed oxygen and carbon dioxide.
  • the discharge hole of the gas supply pipe 71 is opened when the pressure is higher than the set pressure, it is possible to prevent the medium for culturing through the gas supply pipe 71 to flow back through the discharge pipe.
  • the control unit 150 includes an illuminance sensor 140, a dissolved-quantity sensor 130, a temperature sensor 151, a PH sensor 120 and a biomass concentration measuring sensor ( By optimizing the culture atmosphere by the signal detected from 145) it is possible to maximize the culture efficiency.
  • the cultured photobioorganisms are stored in the photobioorganic storage tank through the photobioorganic discharge pipe. This process enables the continuous production of photobioorganisms.
  • the plate-type photobioreactor module and the photobiological culture system using the same are irradiated with light from the outside of the plate-type photobioreactor using the surface light source unit, so that heat generated from the lamp module of the light source unit is cultured. It can be prevented from being delivered directly to the photobiological organism in question.
  • the photobiological culture system according to the present invention can be irradiated to a flat-type photobiological culture vessel using light irradiated from a lamp such as sunlight and a light emitting diode, so that the degree of freedom of design can be increased, and the light guide plate is used. Irradiation of the photobioreactor can maximize the uniformity of light distribution.
  • this photobiological culture system can be easily adjusted in the high concentration culture by microalgae by optimizing the culture conditions of photobiotic organisms, and can increase the spatial efficiency.

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Abstract

L'invention concerne un système de culture photobiologique comprenant : un récipient de culture photobiologique de type panneau plat comprenant des corps de panneau de culture, accouplés les uns aux autres de manière à obtenir un espace de culture dans lequel des organismes photobiologiques peuvent être injectés pour être cultivés, et présentant des nervures support sur la surface externe correspondante à un intervalle prédéterminé, un tuyau d'alimentation en gaz disposé pour pénétrer, dans une direction horizontale, dans l'espace de culture formé par les corps de panneau de culture et pour laisser sortir un gaz d'arrivée, et au moins une cloison formant des tourbillons s'étendant à partir de la paroi interne des corps de panneau de culture dans la direction horizontale et présentant une forme d'arc de manière à générer un tourbillon par rapport à un flux ascendant de gaz alimenté au travers du tuyau d'alimentation en gaz ; et un module de photobioréacteur de type panneau plat doté d'une unité de source lumineuse de surface située sur au moins un côté du récipient de culture photobiologique de type panneau plat de manière à irradier de la lumière pour la culture des organismes photobiologiques.
PCT/KR2013/001451 2013-01-25 2013-02-22 Module de photobioréacteur de type panneau plat et système de culture photobiologique l'utilisant WO2014115924A1 (fr)

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KR10-2013-0008908 2013-01-25

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WO2022090962A3 (fr) * 2020-10-29 2022-06-09 Zolend Kft. Photobioréacteur clos aseptique et sa procédure d'utilisation

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KR102289069B1 (ko) * 2021-04-27 2021-08-12 농업회사법인 상상텃밭 주식회사 용존산소 농도 및 용존이산화탄소 농도 분석을 통한 수경재배 양액 내 조류 농도 추론 장치 및 방법

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JP2007535961A (ja) * 2004-05-06 2007-12-13 グラクソ　グループ　リミテッド バイオリアクター
US20100035343A1 (en) * 2008-08-06 2010-02-11 Cheng Alan T Y System and method for controlling a mammalian cell culture process
KR101222145B1 (ko) * 2011-12-29 2013-01-14 조선대학교산학협력단 태양광을 이용한 광생물 반응기
KR101222696B1 (ko) * 2012-06-19 2013-01-17 조선대학교산학협력단 와류를 형성하는 호형 격벽 구조를 가지는 미세조류 배양용 광생물 반응기

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JP2007535961A (ja) * 2004-05-06 2007-12-13 グラクソ　グループ　リミテッド バイオリアクター
US20100035343A1 (en) * 2008-08-06 2010-02-11 Cheng Alan T Y System and method for controlling a mammalian cell culture process
KR101222145B1 (ko) * 2011-12-29 2013-01-14 조선대학교산학협력단 태양광을 이용한 광생물 반응기
KR101222696B1 (ko) * 2012-06-19 2013-01-17 조선대학교산학협력단 와류를 형성하는 호형 격벽 구조를 가지는 미세조류 배양용 광생물 반응기

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022090962A3 (fr) * 2020-10-29 2022-06-09 Zolend Kft. Photobioréacteur clos aseptique et sa procédure d'utilisation

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