WO2014108758A1 - Dispersion de nanoparticules pour détecter l'état de la peau et kit diagnostique la contenant - Google Patents

Dispersion de nanoparticules pour détecter l'état de la peau et kit diagnostique la contenant Download PDF

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WO2014108758A1
WO2014108758A1 PCT/IB2013/050322 IB2013050322W WO2014108758A1 WO 2014108758 A1 WO2014108758 A1 WO 2014108758A1 IB 2013050322 W IB2013050322 W IB 2013050322W WO 2014108758 A1 WO2014108758 A1 WO 2014108758A1
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Prior art keywords
skin
color
nanoparticles
dispersion
nanoparticle
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PCT/IB2013/050322
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English (en)
Inventor
Samares Chandra BISWAS
Sudipa PANIGRAHI
Kavitha MURUGESAN
Sudheendra U SEETHARAMACHARYA
Suresh Ramamurthi
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Itc Limited
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Priority to PCT/IB2013/050322 priority Critical patent/WO2014108758A1/fr
Publication of WO2014108758A1 publication Critical patent/WO2014108758A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5064—Endothelial cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B2010/0003—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements including means for analysis by an unskilled person
    • A61B2010/0006—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements including means for analysis by an unskilled person involving a colour change

Definitions

  • the present invention relates to nanoparticles dispersion for detection of skin health conditions. More particularly the present invention relates to diagnostic kit for detection of skin dryness, as well as other skin health/conditions associated with skin dryness with the help of nanoparticles. The present invention also relates to method for distinguishing between dry and normal skin conditions.
  • US 2003/0124553 provides a method for determining the predisposition of an individual to a skin condition comprising identifying the profilaggrin alleles present in the genome of an ex vivo sample taken from the individual.
  • Skin conditions include the ability of an individual to produce Natural Moisturizing Factors (NMF), dry skin and/or predisposition to detergent-induced erythema, conditions of scalp such as dandruff.
  • NMF Natural Moisturizing Factors
  • US 2003/0065552 provides a beauty analysis method, comprising: storing in a data structure a plurality of questions related to beauty; enabling a subject to access a system for presenting at least some of the plurality of questions to the subject; presenting to the subject directions for conducting at least one physical self-test; instructing the subject on how to furnish information reflecting results of the self-test; selecting, from the plurality of questions, a subset of questions to be presented to the subject, wherein a number of questions contained in the subset and identities of questions contained in the subset are a function of the furnished information; and presenting the subset of questions to the subject.
  • the skin conditions may include dryness, cellulitis, sweating, aging, wrinkles, melanoma, exfoliation, desquamation, homogeneity of color, creases etc including dandruff.
  • Gemmer et al, 2002 (Fast, Noninvasive Method for Molecular Detection and Differentiation of Malassezia Yeast Species on Human Skin and Application of the Method to Dandruff Microbiology, Journal Of Clinical Microbiology, Sept. 2002, p. 3350-3357) provides a technique involves fluorescent nested PCR of the intergenic transcribed spacer (ITS) ITS I and ITS II region ribosomal gene clusters. All known Malassezia species can be differentiated by unique ITS fragment lengths. The said technique has been used to directly analyze scalp samples from subjects enrolled in a demographic scalp health study. Results for subjects assigned composite adherent scalp flaking scores (ASFS) ⁇ 10 were compared to those for subjects assigned composite ASFS >24.
  • ASFS composite adherent scalp flaking scores
  • Malassezia restricta and M. globosa were found to be the predominant Malassezia species present in both groups. It was found that no evidence of M. furfur in either group, indicating that M. furfur can be eliminated as the causal organism for dandruff. Both groups also showed the presence of non-Malassezia fungi. This method, particularly when it is used in combination with existing fungal ITS databases, is expected to be useful in the diagnosis of multiple other fungal infections.
  • compositions having particles i.e. nanoparticles or microparticles comprising, inorganic element; one or more active ingredient and optionally a release rate-modulating agent, suitable for the delivery of active ingredients to human and animal tissues.
  • US'993 The particles in US'993 have been synthesized by methods such as homogenization including high pressure homogenization, milling including ball milling, high shear wet milling, media milling, precipitation including supercritical fluid process, emulsification diffusion process, sol gel process, chemical or mechanical methods, aerosol flow reactor and the like thereof
  • the active ingredient of US'993 is a cosmetic agent, selected from the group of anti-ageing agents, sunblocking agents, anti- wrinkle agents, moisturizing agents, anti-dandruff agents, anti-UV agents etc and essential oils and perfumes, substance having a hair-care activity, agents for combating hair loss, hair dyes, hair bleaches, reducing agents for permanent waves, hair conditioners, nutrients or combinations thereof.
  • Ketoconazole coated silver nanoparticles-A point antidandruff agent International Journal of Plant Sciences, (July to December, 2009) Vol. 4 Issue 2 : 517- 520 shows the antidandruff activity of ketoconazole coated silver nanoparticles (AgNp) of 4+2 nm towards the dandruff scales collected from human volunteers by disc diffusion method. The study also shows the minimal inhibitory concentration (MIC) of ketoconazole and ketoconazole coated AgNp during incubation with the dandruff causing fungi- Malassezia furfur.
  • MIC minimal inhibitory concentration
  • US 2003/0003070 relates to nanoscale active anti-dandruff ingredients having a particle diameter ranging from 10-500 nm in the production of hair-cosmetic preparations. It has been found in US'070 that the stability of cosmetic hair-care preparations, such as rinses and shampoos for example, is increased and their effectiveness is significantly accelerated and improved by the addition of antidandruff agents providing the antidandruff agents are present in the form of nanoparticles, i.e. particles with a mean diameter of 10 to 500, preferably 50 to 300 and more particularly 100 to 150 nm. However the prior art does not provides the nanoscale particles for diagnosis of skin heath conditions.
  • US 2009/0196926 relates to a composition, a method, and a kit provided for chemical skin peeling, based on nano-structured thixotropic inorganic gels, which have a higher potency and are less irritant of the conventional peeling formulations.
  • the kit comprises by a defatting gel, different types of peeling gels and a neutralizing gel. All the gels of the invention can be easily removed from the skin after the treatment.
  • the thixotropic gels of the invention can be used in chemical skin peeling for the treatment of various cosmetic conditions and dermatological disorders, including dry skin, acne, dandruff, keratoses, age spots, wrinkles and disturbed keratinisation.
  • the prior art only teaches sampling but not diagnosing.
  • US 2003/0108542 provide method, device and system for determining skin type.
  • the method includes a step of applying at least one drop of substance applied directly onto a zone of the skin or on a collector member previously in contact with the zone of the skin.
  • the substance can modify at least one physicochemical property of the surface of the zone or of the collector member exposed to the substance. After the drop has disappeared or been removed, the extent of the surface is evaluated and the skin type is determined as a function of this evaluation.
  • the substance is a colored substance comprises at least one of Erythrosine B, carmine indigo, potassium permanganate, tannins, and oxidation products of polyphenols, melanines, henna, anthocyanes, fluorescent substances, and Dansyl chloride.
  • the prior art does not use nanomaterials to detect the skin health and also does not require uses of dye or oxidizing agent separately to detect the skin health as the prior art.
  • US 2003/0108542 disclose a method, device and system for determining skin type.
  • the method includes a step of applying at least one drop of substance onto a zone of the skin or on a collector member previously in contact with the zone of the skin. After the drop has disappeared or been removed, the extent of the surface is evaluated and the skin type is determined as a function of this evaluation.
  • the prior art also provides device for measurement of extent of surface of skin whose properties have been modified by drop of test substance used, the device can emit light rays towards the skin and pick up the reflected light.
  • the light rays can be produced by at least one laser diode or by at least one light-emitting diode (LED), for example.
  • Such light rays can make it possible, for example, to detect a modification to the reflective or color properties of the surface of the skin or of the collector member exposed to the drop of substance.
  • the device of prior art is only restricted to the use of reflection measurement of light from surfaces of skin.
  • US 6,447,463 provide diagnostic kit for fungal skin infection and method of detection of skin infection caused by fungus.
  • US'463 although provides a different type of kit for detection of skin infections.
  • the main purpose of the kit mentioned in the prior art is to safely collect, store and transport the samples to the analysis site.
  • the specified method of sample collection in US'463 involves the following steps, a. Collecting skin samples using an adhesive tape
  • the adhesive tape will be detached from the slide and the residue left on the slide will be examined through microscopy or by staining.
  • the prior art describes various methods of determining the skin conditions including the method based on genomic analysis of profillaggrin gene and relating the same to dry/dandruff skin.
  • the prior art does not describe particularly the use of nanoparticle for determining the skin health conditions rather it describes the use of nanoparticle as a delivery system to deliver hair dyes and coloring agents.
  • the prior art discloses an algorithm that uses response-correlation analysis for predicting skin conditions.
  • the prior art uses a ribosomal gene based analysis to identify the presence of Malasseizia species on human scalp and also to identify multiple fungal infections.
  • This prior art discloses enhancement of anti-dandruff activity of ketoconazole by coating it with silver nanoparticle.
  • This prior art discloses the use of nanoparticle as a thixotropic gel to collect the samples. This is related to sampling of skin and not to diagnosis.
  • Prior art also provides method of detection of dryness of skin and diagnostic kit for detection of skin infection.
  • the kits of the prior art involves the measurement of skin conditions based on the physicochemical changes (color and size of the spot) in the skin or collector member where the reagent is applied either on skin or a collector member.
  • the prior art provides an instrument disclosing the reflection of light from skin or collector member surface after treatment with reagent.
  • the prior art does not mention the type of LEDs used for the reflection measurement.
  • the instrument is restricted to use only for reflection measurement of light from surfaces most specifically skin, collector member.
  • the present invention involves collecting skin samples using an adhesive tape and directly add the adhesive tape into the test solution.
  • the sample is treated on spot and special storage and sample handling are not required.
  • None of the prior art documents teaches efficient method for determination of skin health conditions by using nanoparticle.
  • the diagnostic kit/ process available for determining skin health using nanomaterials are the conventional color measurements that involves the use of spectrophotometer that mainly involves monitoring absorption of white light at a fixed wavelength that completely depends on the chemical characteristics of the sample (e.g. electronic structure).
  • the present invention provides a method of diagnosing or direct determination of skin health/conditions using nanoparticle through non-invasive way. Unlike the prior art the present system does not require any use of dye or oxidizing agent separately to detect the skin health.
  • the present invention also provides a diagnostic kit that exploits the intensity of the color that is different from the conventional color-measuring instrument.
  • the color measuring kit disclosed in the present invention is different from conventional instruments (spectrophotometer) in the following way.
  • It is an object of the present invention is to overcome the drawbacks of the prior art. It is another object of the present of the present invention is to provide the nanoparticles dispersion for diagnosing skin health conditions.
  • a further object of the present invention is to provide a diagnostic kit for determining skin health conditions.
  • Yet another object of the present invention is to provide a method of detection of skin disease condition and distinguishing between dry and normal skin conditions, as well as other conditions associated with skin dryness.
  • Yet another object of the present invention is to provide method for diagnostic and prognostic for the detection of dandruff caused by Malassezia including other proliferative skin conditions.
  • Yet another object of the present invention is to provide a method for determining the mildness of surfactants on skin that is useful for classifying cosmetic or therapeutic material as hard or soft to skin.
  • nanoparticles dispersion for diagnosing skin health conditions comprising at least three components selected from glucose, chloroauric acid, potassium hydroxide, sodium borohydride, and water, wherein said nanoparticle dispersion has a particle size of 1 to 100 nm.
  • a test kit for diagnosing skin health conditions comprising a sample holder having solution of nanoparticles dispersion comprising at least three components selected from glucose, chloroauric acid, potassium hydroxide, sodium borohydride, and water and the skin test samples; and a detection unit comprising a switch and the color indicators adapted for diagnosing the skin health conditions, said detection unit comprising:
  • micro-controller adapted for controlling the sequence of operations and relays; ii. an interface between output voltage and an analogue to digital converter that connect to the micro-controller;
  • iii a firmware adapted for controlling the operation of the microcontroller
  • a display unit containing color specific LEDs iv. a display unit containing color specific LEDs.
  • step i measuring the color of test samples obtained in step i
  • step b) comparing the level of color intensity of test solution determined in step b) with the level of stored values for corresponding colors; d) determining the color of the solution;
  • Figure 1 illustrates the schematic presentation of the diagnostic kit 1.
  • Figure 2 illustrates the schematic presentation of the diagnostic kit 2.
  • FIG 3 illustrates schematic of the proposed color-measuring instrument.
  • Figure 4 illustrates circuit diagram for the proposed apparatus.
  • Figure 5 illustrates block diagram for the different components of the proposed color- measuring instrument.
  • Figure 6 illustrates detection of skin health using nanoparticles.
  • Figure 7 illustrates spectrophotometric measurement associated with change in color of nanoparticles reflecting the skin conditions/health.
  • Figure 8 illustrates time dependent effect of pH on the UV-Vis spectra of nanoparticles.
  • Figure 9 illustrates time dependent effect of skin type on the UV-Vis spectra of nanoparticles.
  • the present invention is therefore directed to a simple and effective system of detection of the skin health conditions that does not require any biological markers such as antibody.
  • the invention is directed to nanoparticle dispersion used as markers to detect the skin health conditions.
  • the composition of the nanoparticles includes surfactants (cationic or anionic), glucose, chloroauric acid, potassium hydroxide, sodium borohydride, sodium hydroxide and water. It was surprisingly found that out of fifteen different nanoparticle samples, only four compositions of the nanoparticles showed immediate color change with skin samples. It was observed that the nanoparticle composite is able to detect the skin condition by providing the immediate color change comprising at least three components selected from chloroauric acid, sodium borohydride, glucose, potassium hydroxide and water. More preferably, the nanoparticles dispersion comprises water, chloroauric acid, glucose and potassium hydroxide.
  • the nanoparticle dispersion comprises water in about 50-99.9 percent by weight more preferably 90-99.9 percent by weight, chloroauric acid in about 0.001-10 percent by weight, more preferably 0.01-5 percent by weight, glucose in about 0.01-50 percent by weight, more preferably 0.1-10 percent by weight and potassium hydroxide in about 0.001-5 percent by weight, more preferably 0.01-1.0 percent by weight.
  • the particle size of the nanoparticles of present invention varies from 1 to 100 nm, preferably 5-50 nm.
  • Another aspect of the present invention provides a diagnostic kit that uses color measurement for determining skin health/conditions (e.g. dry or normal).
  • the diagnostic kit of the present invention comprises the following components ( Figure 1).
  • A Tip impregnated/immobilized with nanoparticle/mixture of nanoparticles on an absorbing materials (e.g. absorbent paper);
  • Kit 2 Detachable adhesive tape for collecting samples (e.g. skin). Another embodiment of the present invention, there is provided a kit ( Figure 2) comprising the components as
  • A Glass or polymeric transparent bottle containing 0.5 - 1.0 mL of gold nanoparticles dispersion in water;
  • B Cap of the bottle containing the detachable adhesive tape;
  • C Detachable adhesive tape to collect skin samples;
  • D Color shades for matching the color of the nanoparticle pre- and post- application.
  • the color measuring apparatus as shown in Figure 3 in accordance with the present invention mainly consists of
  • Micro-controller that controls the sequence of operations and relays
  • An interface operation amplifier that connect between output voltage and an analogue to digital converter to the micro-controller;
  • Step 1 Collection of skin sample using tape stripping and extraction of water soluble components
  • coloring agents e.g. dye or nanoparticles
  • Step 2 Measuring the color of the skin samples treated with coloring agents
  • step 1 Colored solutions obtained in step 1 were placed in front of multi-colored light emitting diodes/bulbs (LED) that can be controlled/ switched on in a sequence by a relay mechanism using a firmware through the microcontroller as shown in Figure 4.
  • LED light emitting diodes/bulbs
  • the color of the solution is measured using light dependent resistors (LDR) kept on the other side of the sample.
  • LDR light dependent resistors
  • the microcontroller stores the output voltage coming from the LDR for different colors intensity.
  • the microcontroller compares the stored voltage values with the pre - calibrated voltage values for corresponding colors and determine the color of the solution.
  • the results can be displayed as numerical value or as text (e.g. good or bad, dry or normal, dandruff or normal) or as colors.
  • the diagnostic kit of the present invention as shown in the block diagram Figure 5 comprises different components of the color measuring instrument as provided below:
  • Micro-controller reads
  • Microcontroller enables switching on the LEDs (light Emitting Diodes) via relays and LED light pass through the sample solution.
  • E An interface (operations amplifier, AMP) that connects between voltage (Vo) and an analogue to digital converter (ADC) that connects to the micro-controller.
  • AMP operation amplifier
  • ADC an analogue to digital converter
  • Microcontroller compares the signal obtained from ADC with a pre-populated/ pre- calibrated look-up table and determines the color of the light that is coming through the sample.
  • the display unit shows the numerical value 1 to 10, where 1 indicates normal and 10 indicates dryness/dandruff.
  • Color gradient ranges from wine red to purple, violate, light blue, dark blue and colorless.
  • the substrate conditions as herein indicates excellent, very good, good, satisfactory, poor, bad, normal scalp and dandruff scalp.
  • Another aspect of the present invention is directed to a method that can distinguish between dry and normal skin conditions, as well as other skin conditions associated with skin dryness such as dandruff, psoreasis, atopic dermatitis, skin barrier damage and enzymatic (proteages, lipases) malfunctioning etc
  • the method involves change in color of nanoparticles, used in the present invention, due to skin property related to skin conditions.
  • the method of the present invention is utilized for the development of diagnostic kits for determining skin health.
  • the present invention also provides a method for determining the mildness of surfactant on skin that is useful for classifying cosmetic/therapeutic material as hard or soft to skin.
  • the extent of damage of skin barrier function depends on mildness of the surfactant.
  • the invention also provides methods for the diagnostic and prognostic methods for the detection of dandruff caused by Malassezia, including other proliferative skin conditions.
  • the features like surface properties, concentration of the nanoparticles and mildness of surfactants determine the response for skin conditions in the present invention.
  • the present invention discloses different regulating factors for the red shift of ⁇ mentioned as below:
  • the present invention is applicable to both Non-invasive and invasive and both visual and colorimetric i.e. qualitative and quantitative method.
  • the present method can distinguish minute changes of skin conditions.
  • the present invention provides simple and portable system and hence quite consumer friendly for onsite product demo.
  • the present invention provides a cost-effective method that is delivered to the consumer to check the product efficacy.
  • the present invention describes a process where the result can be analyzed based on the change in color of the system.
  • Figure 1 shows diagnostic kit 1 for determining the skin health conditions.
  • Figure 2 shows diagnostic kit 2 for determining the skin health conditions.
  • Figure 3 illustrates Schematic of the proposed color-measuring instrument.
  • Figure 4 illustrates Circuit diagram for the proposed apparatus.
  • Figure 5 illustrates Block diagram for the different components of the proposed color- measuring instrument.
  • Figure 6 shows detection of skin health using nanoparticles in different skin samples. Skin samples were collected using adhesive tapes from volar forearm and elbow and treated with nanoparticles in eppendruff tube (2 ml).
  • Figure 7 shows the spectrophotometric measurement associated with change in color of nanoparticles reflecting the skin conditions/health.
  • Figure 8 shows the time dependent effect of pH on the UV-Vis spectra of nanoparticles. Results show that mere changes in the pH of the medium did not produce the desired response.
  • Figure 9 shows that time dependent effect of skin type on the UV-Vis spectra of nanoparticles.
  • Example 1 The diagnostic kit 1 can operate in two different modes.
  • Mode 1 Added drop/drops of water on test skin (e.g. volar forearm or elbow) and equilibrate for 30 sec to 1 min followed by touching the water drop using tip A.
  • the color change will reflect the skin condition (e.g. dryness, presence of dandruff).
  • Mode 2 Collected skin sample using adhesive tape (C) and disperse the skin sample in water. Dip the tip C in the water and observe the color change.
  • the diagnostic kit 2 can operate by mode 1 as given below:
  • Mode 1 Collect skin sample using adhesive tape (C) and keep the tape containing skin sample back into the bottle containing nanoparticle solution and close the cap and shake for 30-60 sec and observed the color change. Compare the color with the shade provided on the bottle for determining skin condition.
  • C adhesive tape
  • Skin samples were collected using adhesive tapes from volar forearm and elbow and treated with nanoparticles in eppendorf tube (2 ml).
  • Nanoparticles did not show any color change only with tape strips.
  • composition of nanoparticle dispersion according to present invention given in Table 1
  • Step 1 Preparation of nano particles
  • the final concentration of NaOH was 10 "3 M.
  • Step 2 Collection of skin samples
  • Step 4 UV-Vis Spectroscopic analysis of the nanoparticle before and after treatment
  • UV-Vis spectral profile of nanoparticles remained unaltered in the presence of different skin samples.
  • the process of detection comprised of the following steps Step 1: Preparation of nanoparticles
  • CTAB hexadecyltrimethylammonium bromide
  • Step 2 Collection of skin samples
  • Step 4 UV-Vis Spectroscopic analysis of the nanoparticle before and after treatment (i) The UV-Vis spectra for nanoparticle were recorded. The surface plasmon band of the nano particles was observed at 520 nm.
  • UV-Vis spectral profile of nanoparticles remained unaltered in the presence of different skin samples.
  • Step 1 Preparation of nanoparticles
  • Step 2 Collection of skin samples
  • Step 3 Treatment of skin samples with nanoparticles
  • Step 4 UV-Vis Spectroscopic analysis of the nanoparticle before and after treatment i.
  • the UV-Vis spectra for nanoparticle were recorded with and without skin samples.
  • the surface plasmon bands of the nanoparticles were observed at 520 nm.
  • ii The UV-Vis spectra of nanoparticle with only adhesive tape (without any skin samples) were recorded. There was no change in the spectral profile of nanoparticle in the presence of adhesive tape.
  • iii The surface plasmon band of nanoparticle after treatment with volar forearm skin sample was recorded. A red shift in the surface plasmon band was observed after 30 seconds of treatment. The plasmon band was red shifted to 583 nm along with a hump at 531 nm.
  • the surface plasmon band of nanoparticle after treatment with elbow skin sample was recorded. A further red shift in the surface plasmon band was observed after 30 seconds of treatment. The plasmon band was red shifted to 607 nm along with a hump at 531 nm.
  • UV-Vis spectral profile of nanoparticles changed in the presence of skin samples. There is a red shift in the spectral profile depending on the skin conditions/health.
  • the process of detection comprised of the following steps
  • Step 1 Preparation of nanoparticles
  • Step 3 Treatment of skin samples with nanoparticles
  • Step 4 UV-Vis Spectroscopic analysis of the nanoparticle before and after treatment i.
  • the UV-Vis spectra for nanoparticle were recorded.
  • the surface plasmon band of the nano particles were observed at 520 nm.
  • ii. The UV-Vis spectra of nanoparticle with only adhesive tape were recorded. There was no change in the spectral profile of nanoparticle in the presence of only adhesive tape (without any skin samples).
  • iii The surface plasmon band of nanoparticle after treatment with volar forearm skin sample was recorded. A red shift in the surface plasmon band was observed after 30 seconds of treatment. The plasmon band was red shifted to 583 nm along with a hump at 531 nm.
  • the surface plasmon band of nanoparticle after treatment with elbow skin sample was recorded. A further red shift in the surface plasmon band was observed after 30 seconds of treatment. The plasmon band was red shifted to 607 nm along with a hump at 531 nm.
  • UV-Vis spectral profile of nanoparticles changed in the presence of skin samples. There is a red shift in the spectral profile depending on the skin conditions/health.
  • Nanoparticles when treated with skin samples from volar forearm showed a red shift in the ⁇ from 545 nm).
  • UV-Vis spectral analysis will provide quantitative measure of skin health depending on the color change as well as shift in the absorption peaks.
  • the final volume was adjusted to 10ml in water for all the samples.
  • the efficacy of the nanoparticles was determined based on their response time, i.e. time dependent change in color as given in Table 5.
  • the observed response time is the combined effect of compositions and combinations.

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Abstract

Cette invention concerne une dispersion de nanoparticules pour diagnostiquer l'état de la peau comprenant au moins trois composants choisis parmi le glucose, l'acide chloroaurique, l'hydroxyde de potassium, le borohydrure de sodium, et l'eau, ladite dispersion de nanoparticules ayant une taille de particule de 1 à 100 nm. Un kit de test pour diagnostiquer l'état de la peau comme normale, sèche ou squameuse est en outre décrit, ledit kit comprenant une dispersion de nanoparticules comprenant au moins trois composants choisis parmi le glucose, l'acide chloroaurique, l'hydroxyde de potassium, le borohydrure de sodium, et l'eau. L'invention concerne également une méthode diagnostique pour évaluer l'état de la peau comprenant les étapes de collecte d'un échantillon de peau, de traitement dudit échantillon avec la dispersion de nanoparticules et de mesure de la couleur des échantillons d'essai et de diagnostic de peau sèche.
PCT/IB2013/050322 2013-01-14 2013-01-14 Dispersion de nanoparticules pour détecter l'état de la peau et kit diagnostique la contenant WO2014108758A1 (fr)

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WO2018130876A1 (fr) 2017-01-10 2018-07-19 Dukebox Sp. Z O. O. Procédé de fabrication d'une suspension de nanoparticules de sel de potassium ou de sel de magnésium
CN110335655A (zh) * 2019-07-11 2019-10-15 四川大学华西医院 一种住院患者皮肤损伤综合预防管理辅助决策系统及方法
FR3100451A1 (fr) * 2019-09-05 2021-03-12 L'oreal Méthode de diagnostic de peaux sèches
EP3463058B1 (fr) * 2016-05-31 2023-11-15 Indian Institute of Technology, Guwahati Système/kit à base de transmittance pour la quantification au point d'intervention d'échantillons de biomarqueurs et son utilisation

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3463058B1 (fr) * 2016-05-31 2023-11-15 Indian Institute of Technology, Guwahati Système/kit à base de transmittance pour la quantification au point d'intervention d'échantillons de biomarqueurs et son utilisation
WO2018130876A1 (fr) 2017-01-10 2018-07-19 Dukebox Sp. Z O. O. Procédé de fabrication d'une suspension de nanoparticules de sel de potassium ou de sel de magnésium
CN110335655A (zh) * 2019-07-11 2019-10-15 四川大学华西医院 一种住院患者皮肤损伤综合预防管理辅助决策系统及方法
FR3100451A1 (fr) * 2019-09-05 2021-03-12 L'oreal Méthode de diagnostic de peaux sèches

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