WO2014088135A1

WO2014088135A1 - Shampoo composition having anti-inflammatory and anti-allergy effects and hair growth function

Info

Publication number: WO2014088135A1
Application number: PCT/KR2012/010583
Authority: WO
Inventors: 김학수
Original assignee: 주식회사 삼부메디칼
Priority date: 2012-12-06
Filing date: 2012-12-06
Publication date: 2014-06-12

Abstract

A shampoo composition having anti-inflammatory and anti-allergy effects and a hair growth function contains a combination extract of Trachelospermum asiaticum, Melia azedarach Linne var. japonica Makino, and Rosa multiflora thumb. var. multiflora, wherein the content of the combination extract of Trachelospermum asiaticum, Melia azedarach Linne var. japonica Makino, and Rosa multiflora thumb. var. multiflora is 30 to 36 wt.% with respect to the total weight of the composition.

Description

Shampoo composition with anti-inflammatory and anti-allergic effect and hair growth function

The present invention relates to a shampoo composition having anti-inflammatory and anti-allergic effect and hair growth function.

Hair loss has been estimated to be caused mainly by abnormal endocrine system such as hormonal imbalance, autonomic nervous system, circulatory system such as blood circulation disorder, excessive nutrition of hair roots, malnutrition of hair root, allergy, bacterial infection and genetic factors, and aging. In recent years, the cause of environmental factors such as mental stress, air pollution, and food from complex environments has been raised.

In the past, such alopecia mainly appeared in middle-aged people, but recently hair and scalp damage caused by shampoo, mousse, perm, dyeing, dry heat is a significant cause of hair loss is a trend that is increasing significantly in young people in their 20s and 30s. Therefore, the selectivity of the shampoo is known to have a lot of effects on hair loss and hair loss prevention, many functional shampoos for hair loss prevention has been developed and distributed in the present situation, the present inventors also pay attention to the shampoo composition that can prevent hair loss Continued research.

In addition, hair and scalp are part of the body, and as they age, they appear aging. As aging progresses, various changes and problems appear in the hair and scalp.

Therefore, the technical problem to be achieved in the present invention is to ultimately relieve scalp irritation and inflammation, protect hair and strengthen hair roots to prevent hair loss, to provide a method for producing a scalp heat lowering and moisturizing shampoo composition It is to.

In addition, another technical problem to be achieved in the present invention is to provide a scalp heat lowering and moisturizing shampoo composition prepared according to the manufacturing method.

It contains an extract of ground cord, red bark and brier as an active ingredient,

Shampoo composition having anti-inflammatory and anti-allergic effect and hair growth function, characterized in that the cord, geunpi, brier complex extract contained 30 to 36% by weight per total weight of the composition.

The shampoo composition according to the present invention also has an anti-inflammatory and anti-allergic effect and the effect of maintaining hair growth function and moisturizing, and ultimately can reduce hair loss by alleviating scalp irritation and inflammation, protecting hair and strengthening the hair root, It is expected to be very useful in the field.

According to an aspect of the present invention, the present invention provides a cosmetic composition for alleviating skin irritation, which comprises an abrasive line, a medicinal bark, a brier complex extract as an active ingredient. In the embodiment of the present invention, the extract of the cord, hardwood, brier complex with a polar or non-polar solvent and filtered, concentrated and dried to prepare an extract.

In the cosmetic composition for alleviating the skin irritation of the present invention, it is preferable that the abrasive line, the bark and brier complex extract is preferably contained in 30 to 36% by weight per weight of the composition, more preferably 32 to 34 per weight of the composition. It is preferably contained in weight percent. In the formulation example of the present invention, a shampoo containing 10% by weight of the abrasive cord, the bark and brier complex extract prepared in Example 1 was prepared.

The skin stimulating alleviation effect in the cosmetic composition for skin stimulation alleviation of the present invention is characterized in that the cell protective effect against the stimulating source, the anti-inflammatory effect through the inhibition of iNOS, and the anti-allergic effect by the inhibition of histamine secretion can be confirmed. In order to prove this, in the experimental example of the present invention, the skin cell protective effect on the stimulant, the activity of inflammatory mediators and the release of histamine of the allergic reaction were measured. In one case, it was confirmed that the cytoprotective effect on the stimulating agent, the inhibitory effect of inflammatory factors and the histamine release inhibitory effect were excellent. This suggests that the extract of Mahjong, Japonica and Briernab of the present invention can significantly reduce inflammation and allergic reactions and confirm the skin irritation effect by protecting the cells.

In the cosmetic composition for alleviating the skin irritation of the present invention, it is preferable that the extract of the abrasive wire, the bark and brier complex extract is extracted with a polar solvent or a nonpolar solvent. The polar solvent may be any polar solvent that can be used for extracting abrasion cord, bark, brier, but preferably water, methanol, ethanol, butanol or a mixed solvent thereof. In addition, the non-polar solvent may be any non-polar solvent that can be used for extracting abrasion line, bark, brier, but preferably hexane, glycerin, butylene glycol, chloroform or ethyl acetate.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. Since these Examples and Experimental Examples are only for illustrating the present invention, the scope of the present invention is not limited by these Examples and Experimental Examples.

Preparation Example 1

Three times 80% ethanol, dried bark, bark and brier complexes at 70 ° C. were added, extracted at 20 to 70 ° C. for 1 to 3 days, filtered, concentrated in vacuo and freeze-dried to prepare 75% ethanol extract.

Preparation Example 2

Purified water extract was prepared by following the same reaction process as in Preparation Example 1 except for changing 80% ethanol to purified water.

Preparation Example 3

A 30% ethanol extract was prepared by performing the same reaction process as in Preparation Example 1 except that 80% ethanol was changed to 30% ethanol.

Preparation Example 4

A butanol extract was prepared by following the same reaction procedure as in Preparation Example 1 except for changing 80% ethanol to butanol.

Preparation Example 5

A 30% glycerin extract was prepared by performing the same reaction process as in Preparation Example 1 except that 80% ethanol was changed to 30% glycerin.

실험예 1. 세포 배양Experimental Example 1. Cell Culture

All cells of the following experimental example was purchased from ATCC (America Type Culture Collect, USA) and used through subculture. All cells were cultured in a cell incubator (ELITE II, Revco, USA) at 37 ° C., 5% CO ₂ conditions.

Experimental Example 2 Measurement of Cytotoxicity in Dermal Cells of Abrasive Cord, Red Bark and Brier Extract

In order to measure the cytotoxicity of the complex extract, MTT (thiazolyl blue tetrazolium bromide, Sigma, USA) method described in the literature ( J. Immunol. Methods , 141 (1), 15 (1991)) was performed as follows. . It is based on the principle of reducing water-soluble purple MTT formazandm.

After dissolving MTT formazan at room temperature, the absorbance was measured at 570 nm, and the results are shown in Table 1 by comparing the absorbance difference with the negative control.

Table 1

sample	Concentration (㎍ / ml)	Cell viability (% of control)
Control		100
Preparation Example 1.	10	99
	50	99
	100	98
Preparation Example 2.	10	98
	50	99
	100	97
Preparation Example 3.	10	96
	50	97
	100	98
Preparation Example 4.	10	99
	50	97
	100	96
Preparation Example 5.	10	97
	50	96
	100	98

As shown in Table 1, the complex extract was confirmed that there is no toxicity in skin cells.

실험예 3. 마삭줄, 고련피, 찔레꽃 복합 추출물의 iNOS 생성 저해 효과Experimental Example 3. Inhibitory effect of iNOS production by the extracts of horsetail, red bark and brier

RAW 264.7 cultured in Experimental Example 1 was inoculated into a 24 well plate at a density of 8 × 10 ⁵ cells / well, and cultured for 1 day, and then 0.1 ㎍ of LPS (lipopolysaccharide from E.coli , Sigma, USA), an inflammatory response substance / ml containing phenol-red free DMEM was replaced and the extract was added to each concentration was incubated for 1 day. Table 2 shows the results.

TABLE 2

sample	Concentration (㎍ / ml)	iNOS activity rate (% of LPS)
Control		49
LPS	0.1	100
Preparation Example 1.	10	79
	50	69
	100	54
Preparation Example 2.	10	93
	50	85
	100	76
Preparation Example 3.	10	93
	50	80
	100	77
Preparation Example 4.	10	89
	50	82
	100	75
Preparation Example 5.	10	90
	50	82
	100	78

In the results of Table 2, the complex extract was shown to have an anti-inflammatory effect by inhibiting iNOS activity, an inflammatory factor. In particular, the extract of Preparation Example 1 was found to inhibit the iNOS activity very well. Therefore, according to the results of the iNOS activity inhibition experiment, the extract of Preparation Example 1 was determined to be very good for stimulation relaxation, and the following experiment was used to verify the efficacy using the Extract Preparation Example 1.

실험예 4. 마삭줄, 고련피, 찔레꽃 복합 추출물의 세포 보호 효과Experimental Example 4. Cell protection effect of the extracts of horsetail, red bark, brier

In order to confirm the cell protective effect of Preparation Example 1, SDS (sodium dodecyl sulfate, Sigma, USA) was used as a stimulator to measure the cell protective effect. The results are shown in Table 3 below.

TABLE 3

sample	Concentration (㎍ / ml)	Cell survival rate (% of control)
Control		100
SDS		38
Preparation Example 1.	10	49
	50	65
	100	73

As shown in Table 3, the complex extract was confirmed that the cell protection effect on the stimulation source is excellent.

실험예 5. 마삭줄, 고련피, 찔레꽃 복합 추출물의 항알러지 효과Experimental Example 5. Anti-allergic effect of the extracts of horsetail, red bark, brier

Mast cells secrete histamine, which is activated by the immune response and acts as a major cause of allergies. When degranulation occurs in activated mast cells, β-Hexosaminidase is released together with histamine, and β-Hexosaminidase released can be used as an indicator of degranulation ( Planta Med . 64, 577-578 (1998)).

Table 4

sample	Concentration (㎍ / ml)	Degranulation inhibitory effect (% of Control)
Control		100
Preparation Example 1.	10	48
	50	51
	100	72
Quercetin	3	85
Quercetin	10	98

In the results of Table 4, Preparation Example 1 was very excellent in inhibiting the degranulation. These results indicate that the complex extract of the present invention can inhibit allergic induction by inhibiting degranulation of mast cells.

Although the present invention has been described in connection with the above-mentioned preferred embodiments, the scope of the present invention is not limited to these embodiments, and the scope of the present invention is defined by the following claims, and equivalent scope of the present invention. It will include various modifications and variations belonging to.

The reference numerals set forth in the claims below are merely to aid the understanding of the present invention, not to affect the interpretation of the scope of the claims, and the scope of the claims should not be construed narrowly.

Claims

It contains an extract of ground cord, red bark and brier as an active ingredient,

Shampoo composition having anti-inflammatory and anti-allergic effect and hair growth function, characterized in that the cord, geunpi, brier complex extract contained 30 to 36% by weight per total weight of the composition.
The method of claim 1,

The abrasion cord, yampi, brier complex extract is a cosmetic composition for skin irritation relief and prevention, characterized in that extracted with a polar solvent or a non-polar solvent.
The method of claim 4, wherein

The polar solvent is water, methanol, ethanol, butanol or any one of a solvent selected from a mixed solvent thereof, the skin composition for reducing and preventing skin irritation.
The method of claim 4, wherein

The non-polar solvent is any one selected from the group consisting of hexane, glycerin, butylene glycol, chloroform and ethyl acetate skin stimulation and prevention cosmetic composition.