WO2014040394A1 - Flore microbienne complexe et son application - Google Patents

Flore microbienne complexe et son application Download PDF

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Publication number
WO2014040394A1
WO2014040394A1 PCT/CN2013/001095 CN2013001095W WO2014040394A1 WO 2014040394 A1 WO2014040394 A1 WO 2014040394A1 CN 2013001095 W CN2013001095 W CN 2013001095W WO 2014040394 A1 WO2014040394 A1 WO 2014040394A1
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Prior art keywords
raw material
fiber
bacterial liquid
raw materials
bacterial
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PCT/CN2013/001095
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English (en)
Chinese (zh)
Inventor
贾平
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北京天安生物科技有限公司
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Priority claimed from CN201210343133.XA external-priority patent/CN103074259B/zh
Priority claimed from CN201210343855.5A external-priority patent/CN103074264B/zh
Priority claimed from CN201210343134.4A external-priority patent/CN103074260B/zh
Application filed by 北京天安生物科技有限公司 filed Critical 北京天安生物科技有限公司
Publication of WO2014040394A1 publication Critical patent/WO2014040394A1/fr

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    • DTEXTILES; PAPER
    • D01NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01CCHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
    • D01C1/00Treatment of vegetable material
    • D01C1/04Bacteriological retting
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/002Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F7/00Fertilisers from waste water, sewage sludge, sea slime, ooze or similar masses
    • C05F7/005Waste water from industrial processing material neither of agricultural nor of animal origin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • C12N1/205Bacterial isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38Pseudomonas
    • C12R2001/39Pseudomonas fluorescens
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/141Feedstock
    • Y02P20/145Feedstock the feedstock being materials of biological origin

Definitions

  • the invention relates to a composite flora and its application in preparing textile fibers, cellulose for additives and pulping of biological bacteria liquid.
  • hemp fibers have been favored by consumers because of their ecological and environmentally friendly characteristics, and their demand is increasing year by year.
  • the global Tianshuran fiber is growing at an annual rate of 8%.
  • the main characteristics of hemp fiber raw materials are high fiber content, slender fiber is conducive to interweaving, and good strength; small cell cavity, thick cell wall, and large wall-to-cavity ratio; due to the fine cell and fine fiber of hemp fiber, the opacity is high.
  • the disadvantage is that the fibers are not easily separated into filaments, resulting in a fabric having a low gas permeability.
  • the fiber is prepared from the hemp raw material, and the waste liquid generated by the chemical method and the chemical method is polluted, destroys the land, pollutes the air, and has high energy consumption, power consumption and large water consumption.
  • the waste liquid generated in chemical pulp production pollutes the environment, destroys the land and pollutes the air.
  • Waste liquid discharge is large; waste that cannot be reused can only be discharged to the outside. Does not comply with national energy conservation and emission reduction policies. Substances cannot be effectively recycled. Chemical preparations cannot be separated from waste liquids, organic substances are mixed with chemical preparations, and organic substances cannot be reused, resulting in a large loss.
  • bio-fiber technology it is necessary to develop bio-fiber technology to fundamentally solve the above-mentioned pollution problems, save energy and reduce emissions, save water, reduce production costs and increase the use rate of materials.
  • An object of the present invention is to overcome the above-mentioned drawbacks of the prior art chemical fiber-making fibers and to provide a novel biological bacteria for preparing fibers.
  • the inventors obtained a biological bacterium suitable for the production of fibers capable of achieving the above object by a long screening operation.
  • the present invention provides a composite flora comprising Rheinheimem t mgsh memis of the accession number CGMCC No. 5972, Acinetobacter hvoffii of the CGMCC No. 5973 , Pseudomonas fluorescem, deposited under the accession number CGMCC No. 5974.
  • the composite flora can be used for preparing textile fibers, cellulose for additives, and biological bacterial liquid pulping.
  • the invention also provides a method for preparing textile fibers, which mainly comprises the steps -
  • the above-mentioned composite bacterial group is configured as a mixed bacterial aqueous solution according to the following mass ratio, that is, the bacterial liquid:
  • R. jejuni Acinetobacter ruta: Pseudomonas fluorescens is 1-2 : 1-2 : 1-2;
  • Raw material processing cutting the hemp raw material into segments, and swelling the raw materials into the soaking pool; preferably, the hemp raw materials are flax, ramie, yellow kenaf or sisal;
  • Biodegradation Soak the raw material after dissolving into the configured bacterial liquid
  • the biodegradable raw materials are removed from the bacterial liquid, drained, and steamed; the fiber is obtained: the sterilized raw material is subjected to coarse grinding to form a fiber bundle; Two-stage fine grinding to disperse the fiber bundle into a single fiber; screening and filtering the fiber bundle in the slurry after a rough grinding and two fine grinding, and re-pulping to make a single fiber;
  • Drying, carding The fibers obtained above are soaked in warm water, then dried and carded for the preparation of textile fibers.
  • the invention also provides a method for preparing cellulose for additives, which mainly comprises the steps -
  • the above-mentioned composite bacterial group is configured as a mixed bacterial aqueous solution according to the following mass ratio, that is, the bacterial liquid:
  • R. jejuni Acinetobacter ruta: Pseudomonas fluorescens is 1-2 : 1-2 : 1-2;
  • Raw material processing The woody raw materials are peeled and sliced, or the herbal raw materials are cut into segments, and the cut raw materials are placed in a soaking pool to swell;
  • Disintegration squeezing the swollen raw material and/or squeezing
  • Biodegradation Soak the raw material after dissolving into the configured bacterial liquid
  • the biodegradable raw materials are removed from the bacterial liquid, drained, and steamed; the fiber is obtained: the sterilized raw material is subjected to coarse grinding to form a fiber bundle; Two-stage fine grinding to disperse the fiber bundle into a single fiber; screening and filtering the fiber bundle in the slurry after a rough grinding and two fine grinding, and re-pulping to make a single fiber;
  • Sterilization Soak the fiber prepared above in warm water, then dry and sterilize;
  • the sterilized fibers are ground into cellulose as an additive.
  • the invention also provides a biological bacterial liquid pulping method, which mainly comprises the steps -
  • the above-mentioned composite bacterial group is configured as a mixed bacterial aqueous solution according to the following mass ratio, that is, a bacterial strain Liquid - R. sinensis: Acinetobacter sinensis: Pseudomonas fluorescens is 1-2 : 1-2 : 1-2;
  • Raw material processing After peeling or cutting the woody raw materials, or cutting the herbal raw materials into sections, the cut raw materials are put into the soaking pool to swell;
  • Pulping which involves the steps - sparse: squeezing the swollen raw material and/or squeezing:
  • Biodegradation Soak the raw material after dissolving into the configured bacterial liquid
  • Fine refining The above-mentioned one-stage coarse refining is subjected to two-stage fine refining to disperse the fiber bundle into a single fiber; slurry screening: screening, filtering through a slurry after a coarse refining and two-stage fine refining The fiber bundle is repulped and made into a single fiber;
  • Submersible, washing The pulp obtained above is soaked in warm water and used for making cardboard.
  • the density of the bacterial liquid formed in the step 1) is 60 million / ml or more.
  • step 2) the swelling time is 10-12h.
  • the biodegradation temperature of step 3 is maintained at 35-40 ° C for 32-36 hours.
  • the mass ratio of the raw material to the bacterial liquid after the disintegration is 1: 6-9.
  • the steam sterilization is autoclaved for 10-30 minutes.
  • step 2) the soaking liquid after soaking the raw material is flocculated and precipitated, and the supernatant is recovered and reused, and the precipitate is input into the biogas tank to be fermented to generate biogas.
  • the biological method provided by the invention has the advantages of: 1) no pollution to the environment: the waste liquid is directly converted into an organic fertilizer, achieving zero discharge and zero pollution. 2)
  • the biological method protects the fiber. Compared with the conventional chemical method, the method can recover both the whole fiber and the half fiber, thereby increasing the yield. 3) Biological methods are degraded under normal pressure, energy saving, emission reduction, and low carbon. 4) Low production cost and high economic efficiency.
  • the by-product of the present invention is sent to a sedimentation tank for flocculation and sedimentation, and the supernatant liquid is returned to secondary use, and then used as a pre-dip liquid.
  • the floc is rich in a variety of organic matter and phytonutrients such as N, P, K, and the floc is mixed with the old bacterial liquid (multiple degradation of the raw viscous liquid, also containing N, P, K, Fe and trace elements). , acidified, and then discharged into the biogas fermentation tank to produce biogas.
  • the biogas residue, the biogas slurry and the pulverized boiler ash are mixed and granulated to form a granular organic fertilizer, and finally discharged to the factory to achieve zero discharge.
  • the present invention further improves the existing techniques for preparing cellulose and biopulping by providing the above-mentioned biological bacteria obtained by the inventor after a long period of creative labor, which reduces the reaction time and improves the purity and yield of the obtained fiber.
  • the invention applies the biological bacteria to degrade the plant to obtain the fiber in a short time, and the biological bacteria can degrade the lignin in the plant body to produce pulp and paper in a short time, and vice
  • the product is converted into biogas twice, and the biogas is supplied to the coal and gas boiler for combustion and heating, saving coal consumption.
  • biogas residue is made into organic fertilizer, which forms a new economic cycle of “substance organic transformation”, which achieves no waste discharge, that is, zero emissions.
  • substance organic transformation which achieves no waste discharge, that is, zero emissions.
  • FIG. 1 is a flow chart of preparing a textile fiber according to an embodiment of the present invention
  • FIG. 2 is a flow chart of preparing cellulose for an additive according to an embodiment of the present invention.
  • FIG. 3 is a flow chart of preparing cellulose for an additive according to another embodiment of the present invention.
  • FIG. 4 is a flow chart of a biological bacterial liquid pulping method according to an embodiment of the present invention.
  • Figure 5 is a flow chart of a biological bacterial liquid pulping process in accordance with another embodiment of the present invention.
  • the biological bacteria used in the present invention was deposited on April 6, 2012 at the General Microbiology Center of the China Microbial Culture Collection Management Committee (CGMCC, No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing), which contains the deposit number CGMCC. No. 5972, I. Rheinheimem tangshanensis, Acinetobacter Iwoffii, CGMCC No. 5973, Pseudomonas fluorescens, deposited under CGMCC No. 5974.
  • CGMCC China Microbial Culture Collection Management Committee
  • the above complex bacteria group is arranged as a mixed bacteria aqueous solution according to the following mass ratio, that is, the bacterial liquid:
  • R. jejuni Acinetobacter sinensis: Pseudomonas fluorescens is 1 -2: 1 -2: 1-2;
  • the density of the formed bacterial liquid is 60 million / ml or more, and is reserved.
  • the composite flora was configured according to the following mass ratio: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens was 1: 1:2.
  • the flow of the fiber preparation method is divided into three stages: preparation stage, fiber section and sub-wide section.
  • the biogas tank is fermented to produce biogas.
  • Biodegradation The decomposed raw materials are input into the biological bacteria degradation tank or tank, and immersed in the bacterial liquid prepared in Example 1.
  • the mass ratio of the decomposed raw materials to the bacterial liquid is 1:8, and the temperature is maintained at 35- 40 ° C, time 32-36 hours.
  • the degradation reaction occurs under the conditions of the biological bacteria, and its specific effect is exerted.
  • Re-screening Screening and filtering the fiber bundles in a slurry after a coarse grinding and a second-stage fine grinding, and re-pulping them to make a single fiber.
  • the raw material residues separated in A and 3 are rich in nutrients and fermented into cattle and sheep feed.
  • the liquid After immersing and washing the liquid in B and 4, the liquid is turbid, and after flocculation and sedimentation, the supernatant is recovered and reused.
  • the sediment is input into the biogas tank to produce biogas, which is fed into the gas dual-purpose boiler for fuel, which can save energy. Consumption.
  • Bio-organic fertilizer The biogas residue and biogas slurry fermented by the biogas tank are rich in bio-organic fertilizer.
  • the liquid is used as crop topdressing and flower nutrient solution, and solid granulation is used as base fertilizer, which is green fertilizer.
  • the specific preparation process is the same as in Example 2.
  • the difference is that the complex flora is configured according to the following mass ratios: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens 1:2:2; Biodegradable raw materials and bacteria after decontamination
  • the mass ratio of the liquid is 1:7.
  • the specific preparation process is the same as in Example 2.
  • the difference is that the complex flora is configured according to the following mass ratios: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens is 2:1:2; Biodegradable raw materials and bacteria after decontamination
  • the mass ratio of the liquid is 1:8.5.
  • the measurement results of the physical properties of the obtained fibers are shown in Table 1.
  • the specific preparation process is the same as in Example 2.
  • the difference is that the complex flora is configured according to the following mass ratios: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens 1:2:2; Biodegradable raw materials and bacteria after decontamination
  • the mass ratio of the liquid is 1:9.
  • the preparation method of cellulose when using woody raw materials is specifically described.
  • the preparation process of the remaining woody materials, such as poplar and willow, can be carried out by referring to the process.
  • the complex flora is configured according to the following mass ratios: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens 1:2:2; When poplar is used, the complex flora is as follows: Mass ratio configuration: R.
  • jejuni Acinetobacter ruta: Pseudomonas fluorescens 1:2:2; when using willow, the complex flora is configured according to the following mass ratio: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens is 2:1:2.
  • the above four steps can be intermittent or interlocking.
  • Biodegradation The decomposed raw materials are input into the biological bacteria degradation tank or tank, and immersed in the bacterial liquid prepared in Example 1.
  • the mass ratio of the decomposed raw materials to the bacterial liquid is 1:6, and the temperature is maintained at 35- 40 ° C, time 32-36 hours.
  • the lignin reaction is degraded under the conditions of biological bacteria, and its specific effect is exerted.
  • the sterilized raw material is subjected to a rough grinding to form a fiber bundle; the above-mentioned one-stage coarse grinding is subjected to two-stage fine grinding to disperse the fiber bundle into a single fiber; screening, filtering through a rough grinding and two The fiber bundle in the finely ground slurry is reground to make a single fiber.
  • the caragana peels separated in A and 3 are rich in nutrients and fermented into cattle and sheep feed.
  • the liquid After immersing and washing the liquid in B and 4, the liquid is turbid, and after flocculation and sedimentation, the supernatant is recovered and reused.
  • the sediment is input into the biogas tank to produce biogas, which is fed into the gas dual-purpose boiler for fuel, which can save energy. Consumption.
  • Bio-organic fertilizer The biogas residue and biogas slurry fermented by the biogas tank are rich in bio-organic fertilizer.
  • the liquid is used as crop topdressing and flower nutrient solution, and solid granulation is used as base fertilizer, which is green fertilizer.
  • Example 7 Preparation of Cellulose from Herbs
  • the wheat straw is used as a raw material, and the preparation method of the cellulose when the herbal raw material is used is specifically described.
  • the preparation process of the remaining herbal materials, such as straw and reed, can be carried out by referring to the process.
  • the composite flora When wheat straw is used, the composite flora is configured according to the following mass ratios: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens is 2:1:2; when straw is used, the composite flora is as follows: Mass ratio configuration: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens is 2:2:2; When reeds are used, the complex flora is configured according to the following mass ratio: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens is 1:1:3.
  • the biological bacterial liquid pulping method when using woody raw materials is specifically described.
  • the biopulping process of the remaining woody materials such as poplar and eucalyptus, can be carried out with reference to the process.
  • the complex flora is configured according to the following mass ratios: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens 1:2:2; When poplar is used, the complex flora is as follows: Mass ratio configuration: R.
  • jejuni Acinetobacter ruta: Pseudomonas fluorescens is 2:1:2; When eucalyptus is used, the complex flora is configured according to the following mass ratio: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens is 2:1:2.
  • the above four steps can be intermittent or interlocking.
  • Disintegration The swollen branches are fed into a silk reeling machine or squeezed and smashed to modify the wood structure to loosen into a wood-like shape, which is beneficial to the penetration of biological bacteria and exerts its degradation effect.
  • Biodegradation The decomposed raw materials are input into the biological bacteria degradation tank or tank, and immersed in the bacterial liquid prepared in Example 1.
  • the mass ratio of the decomposed raw materials to the bacterial liquid is 1:6, and the temperature is maintained at 35- 40 ° C, time 32-36 hours.
  • the lignin reaction is degraded under the conditions of biological bacteria, and its specific effect is exerted.
  • Fine refining From the above-mentioned crude slurry, it is then transferred to a high-concentration refiner for fine refining to disperse the fiber bundle into a single fiber.
  • Slurry screening After two stages of refining, the slurry contains a small number of fiber bundles, which are screened, filtered, and re-slurry to reach a single fiber.
  • Paperboard The paper pulp prepared above is fed into a paper machine, and paperboard is produced by a papermaking process.
  • the caragana peels separated in A and 3 are rich in nutrients and fermented into cattle and sheep.
  • the liquid After immersing and washing the liquid in B and 4, the liquid is turbid, and after flocculation and sedimentation, the supernatant is recovered and reused.
  • the sediment is input into the biogas tank to produce biogas, which is fed into the gas dual-purpose boiler for fuel, which can save energy. Consumption.
  • Bio-organic fertilizer The biogas residue and biogas slurry fermented by the biogas tank are rich in bio-organic fertilizer. The liquid is used as crop topdressing and flower nutrient solution, and solid granulation is used as base fertilizer, which is green fertilizer.
  • Table 4 The results of measuring the physical properties of the paper after refining are detailed in Table 4 below. The data have reached the AA grade of corrugated paper (Table 6):
  • the biological bacterial liquid pulping method when using herbal raw materials is specifically described.
  • the biopulping of the remaining herbal materials, such as wheat straw, straw, and corn stalk, can be carried out with reference to the process.
  • the composite flora When wheat straw is used, the composite flora is configured according to the following mass ratios: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens is 2:1:2; when straw is used, the composite flora is as follows: Mass ratio configuration: R. jejuni: Acinetobacter ruta: Pseudomonas fluorescens is 3:1:2; When using corn stalks, the complex flora is configured according to the following mass ratio: R. jejuni : Acinetobacter ruta: Pseudomonas fluorescens is 2:2:1.
  • the process of the biological bacterial slurry method is divided into three stages: preparation stage, pulping section and by-product section.
  • the tightness is not less than g/cm 3 AA 0.53 0.50 0.45
  • Lateral ring pressure index is not less than

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Abstract

L'invention concerne une flore microbienne complexe et un procédé d'applicationde celle-ci dans la préparation d'une fibre textile, d'une cellulose destinée à être utilisée en tant qu'additif, et d'une pâte de solution bactérienne biologique. La flore microbienne complexe comprend Rheinheimera tangshanensis déposée sous le numéro CGMCC No 5972, Acinetobacter Iwoffii déposée sous le numéro CGMCC No 5973 et Pseudomonas fluorescens déposée sous le numéro CGMCC No 5974. Le procédé comprend: la formulation d'une solution bactérienne, le traitement des matières premières, et la préparation ou la trituration de la fibre.
PCT/CN2013/001095 2012-09-17 2013-09-16 Flore microbienne complexe et son application WO2014040394A1 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
CN201210343855.5 2012-09-17
CN201210343133.XA CN103074259B (zh) 2012-09-17 2012-09-17 一种复合菌群及其在制备纺织用纤维中的应用
CN201210343855.5A CN103074264B (zh) 2012-09-17 2012-09-17 一种复合菌群及其生物菌液制浆法
CN201210343134.4 2012-09-17
CN201210343134.4A CN103074260B (zh) 2012-09-17 2012-09-17 一种复合菌群及其在制备添加剂用纤维素中的应用
CN201210343133.X 2012-09-17

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WO2014040394A1 true WO2014040394A1 (fr) 2014-03-20

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102002468A (zh) * 2010-08-13 2011-04-06 东华大学 一种荧光假单胞菌da4菌株及其获得方法和应用
CN103074260A (zh) * 2012-09-17 2013-05-01 贾平 一种复合菌群及其在制备添加剂用纤维素中的应用
CN103074264A (zh) * 2012-09-17 2013-05-01 贾平 一种复合菌群及其生物菌液制浆法
CN103074259A (zh) * 2012-09-17 2013-05-01 贾平 一种复合菌群及其在制备纺织用纤维中的应用

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Publication number Priority date Publication date Assignee Title
CN102002468A (zh) * 2010-08-13 2011-04-06 东华大学 一种荧光假单胞菌da4菌株及其获得方法和应用
CN103074260A (zh) * 2012-09-17 2013-05-01 贾平 一种复合菌群及其在制备添加剂用纤维素中的应用
CN103074264A (zh) * 2012-09-17 2013-05-01 贾平 一种复合菌群及其生物菌液制浆法
CN103074259A (zh) * 2012-09-17 2013-05-01 贾平 一种复合菌群及其在制备纺织用纤维中的应用

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ZHANG, XIAOXIA: "Studies on Polyphasic Taxonomy of Rice Root-associated Bacteria and Description of Rheinheimera Tangshanensis sp. nov.", AGRICULTURE, CHINA DOCTORAL DISSERTATIONS FULL-TEXT DATABASE, 2009, pages D043-4 *

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