WO2014040382A1 - Solution bactérienne biologique et son application - Google Patents

Solution bactérienne biologique et son application Download PDF

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Publication number
WO2014040382A1
WO2014040382A1 PCT/CN2013/001083 CN2013001083W WO2014040382A1 WO 2014040382 A1 WO2014040382 A1 WO 2014040382A1 CN 2013001083 W CN2013001083 W CN 2013001083W WO 2014040382 A1 WO2014040382 A1 WO 2014040382A1
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raw material
bacterial liquid
fiber
raw materials
bacterial
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PCT/CN2013/001083
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English (en)
Chinese (zh)
Inventor
贾平
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北京天安生物科技有限公司
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Priority claimed from CN2012103421658A external-priority patent/CN103074214A/zh
Priority claimed from CN201210342705.2A external-priority patent/CN103074257B/zh
Priority claimed from CN201210342545.1A external-priority patent/CN103074252B/zh
Application filed by 北京天安生物科技有限公司 filed Critical 北京天安生物科技有限公司
Priority to CA2885474A priority Critical patent/CA2885474A1/fr
Publication of WO2014040382A1 publication Critical patent/WO2014040382A1/fr

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    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
    • D21H21/00—Non-fibrous material added to the pulp, characterised by its function, form or properties; Paper-impregnating or coating material, characterised by its function, form or properties
    • D21H21/14—Non-fibrous material added to the pulp, characterised by its function, form or properties; Paper-impregnating or coating material, characterised by its function, form or properties characterised by function or properties in or on the paper
    • D21H21/18—Reinforcing agents
    • D21H21/20—Wet strength agents
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F7/00—Fertilisers from waste water, sewage sludge, sea slime, ooze or similar masses
    • C05F7/005—Waste water from industrial processing material neither of agricultural nor of animal origin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
    • D01C1/00—Treatment of vegetable material
    • D01C1/04—Bacteriological retting
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
    • D21H21/00—Non-fibrous material added to the pulp, characterised by its function, form or properties; Paper-impregnating or coating material, characterised by its function, form or properties
    • D21H21/14—Non-fibrous material added to the pulp, characterised by its function, form or properties; Paper-impregnating or coating material, characterised by its function, form or properties characterised by function or properties in or on the paper
    • D21H21/36—Biocidal agents, e.g. fungicidal, bactericidal, insecticidal agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas
    • C12R2001/39—Pseudomonas fluorescens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/141—Feedstock
    • Y02P20/145—Feedstock the feedstock being materials of biological origin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Definitions

  • the invention relates to a biological bacterial liquid and the use thereof in preparing textile fibers, cellulose for additives and pulping of biological bacteria liquid.
  • hemp fiber has been favored by consumers because of its ecological and environmental protection characteristics. Its demand is increasing year by year, and the global natural fiber growth rate is 8% per year.
  • the main characteristics of hemp fiber raw materials are high fiber content, slender fiber is conducive to interweaving, and good strength; small cell cavity, thick cell wall, and large wall-to-cavity ratio; due to the fine cell and fine fiber of hemp fiber, the opacity is high.
  • the disadvantage is that the fibers are not easily separated into filaments, resulting in a fabric having a low gas permeability.
  • the fiber is prepared from the hemp raw material, and the waste liquid generated by the chemical method and the chemical method is polluted, destroys the land, pollutes the air, and has high energy consumption, power consumption and large water consumption.
  • the waste liquid generated in chemical pulp production pollutes the environment, destroys the land and pollutes the air.
  • Waste liquid discharge is large; waste that cannot be reused can only be discharged to the outside. Does not comply with national energy conservation and emission reduction policies. Substances cannot be effectively recycled. Chemical preparations cannot be separated from waste liquids, organic substances are mixed with chemical preparations, and organic substances cannot be reused, resulting in a large loss.
  • bio-fiber technology it is necessary to develop bio-fiber technology to fundamentally solve the above-mentioned pollution problems, save energy and reduce emissions, save water, reduce production costs and increase the use rate of materials.
  • An object of the present invention is to overcome the above-mentioned drawbacks of the prior art chemical fiber-making fibers and to provide a novel biological bacteria for preparing fibers.
  • the inventors obtained a biological bacterium suitable for the production of fibers capable of achieving the above object by a long screening operation.
  • the present invention provides a composite flora comprising Rheinheimera tangshanensis having a deposit number of CGMCC No. 5972 and Pseudomonas fluorescens ⁇ f3 ⁇ 43 ⁇ 4 under the accession number CGMCC No. 5974 ⁇ Wickerhamomyces anomal s of CGMCC No. 5975 (The Wickerhamomyces anomalus is cultured in wort liquid medium at 25 °C for three days, the cells are spherical, ovoid, and sausage-shaped, the size is (4.8-14.4) X (3.6-7.2) Um, precipitated.
  • the composite flora can be used for preparing textile fibers, cellulose for additives, and biological bacterial liquid pulping.
  • the invention also provides a method for preparing textile fibers, which mainly comprises the steps of:
  • the above-mentioned composite bacterial group is configured as a mixed bacterial aqueous solution according to the following mass ratio, that is, the bacterial liquid:
  • raw material processing cutting the hemp raw material into segments, and putting the raw materials into the soaking pool to swell; preferably, the hemp raw materials are flax, ramie, yellow kenaf or sisal;
  • Biodegradation Soak the raw material after dissolving into the configured bacterial liquid
  • the biodegradable raw materials are removed from the bacterial liquid, drained, and steamed; the fiber is obtained: the sterilized raw material is subjected to coarse grinding to form a fiber bundle; Two-stage fine grinding to disperse the fiber bundle into a single fiber; screening and filtering the fiber bundle in the slurry after a rough grinding and two fine grinding, and re-pulping to make a single fiber;
  • Drying, carding The fibers obtained above are soaked in warm water, then dried and carded for the preparation of textile fibers.
  • the invention also provides a method for preparing cellulose for additives, which mainly comprises the steps of:
  • the above-mentioned composite bacterial group is configured as a mixed bacterial aqueous solution according to the following mass ratio, that is, the bacterial liquid:
  • Raw material processing After peeling the woody raw materials, or cutting the herbal raw materials into segments, the cut raw materials are placed in the soaking pool to swell;
  • Disintegration squeezing the swollen raw material and/or squeezing
  • Biodegradation Soak the raw material after dissolving into the configured bacterial liquid
  • the biodegradable raw materials are removed from the bacterial liquid, drained, and steamed; the fiber is obtained: the sterilized raw material is subjected to coarse grinding to form a fiber bundle; Two-stage fine grinding to disperse the fiber bundle into a single fiber; screening and filtering the fiber bundle in the slurry after a rough grinding and two fine grinding, and re-pulping to make a single fiber;
  • Sterilization Soak the fiber prepared above in warm water, then dry and sterilize;
  • the sterilized fibers are ground into cellulose as an additive.
  • the invention also provides a biological bacterial liquid pulping method, which mainly comprises the steps of:
  • the above-mentioned composite bacterial group is configured as a mixed bacterial aqueous solution according to the following mass ratio, that is, a bacterial strain R. jejuni: Pseudomonas fluorescens: Wickerhamomyces anomalus 1-3: 1-2: 2-3;
  • Raw material processing After peeling the woody raw materials, or cutting the herbal raw materials into segments, the cut raw materials are placed in the soaking pool to swell;
  • Disintegration squeezing the swollen raw material and/or squeezing
  • Biodegradation Soak the raw material after dissolving into the configured bacterial liquid
  • Fine refining The above-mentioned one-stage coarse refining is subjected to two-stage fine refining to disperse the fiber bundle into a single fiber; slurry screening: screening, filtering through a slurry after a coarse refining and two-stage fine refining The fiber bundle is repulped and made into a single fiber;
  • Submersible, washing The paper pulp prepared above is soaked in warm water for making cardboard.
  • the density of the bacterial liquid formed in the step 1) is 6 X 10 7 /ml or more.
  • the swelling time is 10-12 h.
  • Step 3) The biodegradation temperature is maintained at 35-40 ° C for 32-36 hours.
  • the mass ratio of the raw material to the bacterial liquid after the disintegration is 1:6-9.
  • the steam sterilization is autoclaved for 10-30 minutes.
  • step 2) the soaking liquid after soaking the raw material is flocculated and precipitated, and the supernatant is recovered and reused, and the precipitate is input into the biogas tank to be fermented to generate biogas.
  • the biological method provided by the invention has the advantages of: 1) no pollution to the environment: the waste liquid is directly converted into an organic fertilizer, achieving zero discharge and zero pollution. 2)
  • the biological method can protect the fiber. Compared with the traditional chemical method, the method can recover both the whole fiber and the half fiber, thereby increasing the yield. 3) Biological methods are degraded under normal pressure, energy saving, emission reduction, and low carbon. 4) Low production costs and high economic returns.
  • the by-product of the present invention is sent to a sedimentation tank for flocculation and sedimentation, and the supernatant liquid is returned to secondary use, and then used as a pre-dip liquid.
  • the floc is rich in a variety of organic matter and phytonutrients such as N, P, K, and the floc is mixed with the old bacterial liquid (multiple degradation of the raw viscous liquid, also containing N, P, K, Fe and trace elements). , acidified, and then discharged into the biogas fermentation tank to produce biogas.
  • the biogas residue, the biogas slurry and the pulverized boiler ash are mixed and granulated to form a granular organic fertilizer, and finally discharged to the factory to achieve zero discharge.
  • the present invention further improves the existing techniques for preparing cellulose and biopulping by providing the above-mentioned biological bacteria obtained by the inventor after a long period of creative labor, which reduces the reaction time and improves the purity and yield of the obtained fiber.
  • This enables the technology to be widely applied in actual production.
  • the application of the biological bacteria degrades the plant to obtain the fiber in a short time, and the biological bacteria degrade the lignin in the plant body to produce pulp and paper in a short time, and the by-product is converted into biogas twice, and the biogas is supplied to the coal and gas boiler for combustion and heating. Save coal consumption.
  • FIG. 1 is a flow chart of preparing textile fibers in accordance with an embodiment of the present invention
  • FIG. 2 is a flow chart of preparing cellulose for an additive according to an embodiment of the present invention.
  • FIG. 3 is a flow chart of preparing cellulose for an additive according to another embodiment of the present invention.
  • FIG. 4 is a flow chart of a biological bacterial liquid pulping method according to an embodiment of the present invention.
  • Figure 5 is a flow chart of a biological bacterial liquid pulping process in accordance with another embodiment of the present invention.
  • the biological bacteria used in the present invention were deposited on April 6, 2012 at the General Microbiology Center of China Microbial Culture Collection Management Committee (CGMCC, No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing), which contains the deposit number CGMCC. Rheinheimem tangshanensis No. 5972, Pseudomonem aces, which is deposited under the accession number CGMCC No. 5974, is a fluorescent, and has a deposit number of CGMCC No. 5975.
  • CGMCC General Microbiology Center of China Microbial Culture Collection Management Committee
  • the above complex bacteria group is arranged as a mixed bacteria aqueous solution according to the following mass ratio, that is, the bacterial liquid:
  • R. jejuni Pseudomonas fluorescens: Wtote flmomyces imom Zi ⁇ is 1-2: 1-2: 2-3;
  • the density of the formed bacterial liquid is 6 X 10 7 /ml or more, and is reserved.
  • the complex flora is configured according to the following mass ratio: Tangshan Iwoma/i « is 1:2:3.
  • the flow of the fiber preparation method is divided into three stages: preparation stage, fiber section and by-product section.
  • Biodegradation The decomposed raw materials are input into the biological bacteria degradation tank or tank, and immersed in the bacterial liquid prepared in Example 1.
  • the mass ratio of the decomposed raw materials to the bacterial liquid is 1:8, and the temperature is maintained at 3540°. C, time 32-36 hours.
  • the degradation reaction occurs under the conditions of the biological bacteria, and its specific effect is exerted.
  • Re-screening Screening and filtering the fiber bundles in a slurry after a coarse grinding and a second-stage fine grinding, and re-grinding them into individual fibers.
  • the raw material residues separated in A and 3 are rich in nutrients and fermented into cattle and sheep feed.
  • the liquid After immersing and washing the liquid in B and 4, the liquid is turbid, and after flocculation and sedimentation, the supernatant is recovered and reused.
  • the sediment is input into the biogas tank to produce biogas, which is fed into the gas dual-purpose boiler for fuel, which can save energy. Consumption.
  • Bio-organic fertilizer The biogas residue and biogas slurry fermented by the biogas tank are rich in bio-organic fertilizer.
  • the liquid is used as crop topdressing and flower nutrient solution, and solid granulation is used as base fertilizer, which is green fertilizer.
  • the specific preparation process is the same as in Example 2.
  • the difference is that the composite flora is configured according to the following mass ratios: R. jejuni: Pseudomonas fluorescens: Htot /wmomyces imiw ⁇ Ms is 1:2:2; when biodegraded, the raw materials after decontamination The mass ratio of the bacterial liquid is 1:7.
  • the specific preparation process is the same as in Example 2.
  • the difference is that the composite flora is configured according to the following mass ratios: R. jejuni: Pseudomonas fluorescens: Mfete iimomyc anomalus is 2:2:2; biodegradable, decomposed raw materials and bacterial liquid The mass ratio is 1:8.5.
  • the specific preparation process is the same as in Example 2.
  • the difference is that the complex flora is configured according to the following mass ratio: R. jejuni: Pseudomonas fluorescens: ⁇ 3 ⁇ 4 ⁇ / ⁇ /73 ⁇ 4> «> ⁇ ? ⁇ 0/ ⁇ / ⁇ is 1:2 :3 ;
  • the mass ratio of the raw material to the bacterial liquid after the disintegration is 1:9.
  • the complex flora is configured according to the following mass ratio:
  • the composite flora is configured according to the following mass ratio:
  • the composite flora is configured according to the following mass ratio:
  • the above four steps can be intermittent or interlocking.
  • Disintegration The swollen branches are fed into a silk reeling machine or squeezed and smashed to modify the wood structure to loosen into a wood-like shape, which is beneficial to the penetration of biological bacteria and exerts its degradation effect.
  • Biodegradation The decomposed raw materials are input into the biological bacteria degradation tank or tank, and immersed in the bacterial liquid prepared in Example 1. The mass ratio of the decomposed raw materials to the bacterial liquid is 1:6, and the temperature is maintained at 35- 40 ° C, time 32-36 hours. The lignin reaction is degraded under the conditions of biological bacteria, and its specific effect is exerted.
  • Steam sterilization After the raw material pretreatment is completed, the raw materials are taken out from the bacterial liquid, drained, and input into a steam storage tank, and sterilized by flowing water vapor at normal pressure for 10-30 minutes. The lower end feeds the raw material into the refiner.
  • the sterilized raw material is subjected to a rough grinding to form a fiber bundle; the above-mentioned one-stage coarse grinding is subjected to two-stage fine grinding to disperse the fiber bundle into a single fiber; screening, filtering through a rough grinding and two The fiber bundle in the finely ground slurry is reground to make a single fiber.
  • the caragana peels separated in A and 3 are rich in nutrients and fermented into cattle and sheep feed.
  • the liquid After immersing and washing the liquid in B and 4, the liquid is turbid, and after flocculation and sedimentation, the supernatant is recovered and reused.
  • the sediment is input into the biogas tank to produce biogas, which is fed into the gas dual-purpose boiler for fuel, which can save energy. Consumption.
  • Bio-organic fertilizer The biogas residue and biogas slurry fermented by the biogas tank are rich in bio-organic fertilizer. The liquid is used as crop topdressing and flower nutrient solution, and solid granulation is used as base fertilizer, which is green fertilizer.
  • Table 2 Caragana poplar willow planting degree 560 530 510 Apparent specific volume cm 3 /g 7.3 7.3 7.1 Average particle size ⁇ 210 180 200
  • the wheat straw is used as a raw material, and the preparation method of the cellulose when the herbal raw material is used is specifically described.
  • the preparation process of the remaining herbal materials, such as straw and reed, can be carried out by referring to the process.
  • the composite flora is configured according to the following mass ratio:
  • the composite flora is configured according to the following mass ratio:
  • the composite flora When reed is used, the composite flora is configured according to the following mass ratio: R. jejuni: Pseudomonas fluorescens: Wickerhamomyces momalus is 1 ⁇ 3:2.
  • Table 3 The results of the physical properties of the obtained cellulose are shown in Table 3 below: Table 3 Wheat straw reeds polymerization degree 490 450 450 Apparent specific volume cm 3 /g 7 6.6 6.9 Average particle size ⁇ 190 150 170 Example 8 Wood raw materials Biological bacterial pulping
  • the complex flora is configured according to the following mass ratio:
  • the composite flora is configured according to the following mass ratio:
  • the composite flora is configured according to the following mass ratio:
  • the flow of the biological bacterial slurry method is divided into three stages: preparation stage, pulping section and by-product section.
  • the above four steps can be intermittent or interlocking.
  • Disintegration The swollen branches are fed into a silk reeling machine or squeezed and smashed to modify the wood structure to loosen into a wood-like shape, which is beneficial to the penetration of biological bacteria and exerts its degradation effect.
  • Biodegradation The decomposed raw materials are input into the biological bacteria degradation tank or tank, and immersed in the bacterial liquid prepared in the first embodiment.
  • the mass ratio of the raw materials to the bacterial liquid after the disintegration is 1:6, and the temperature is maintained at 35 ⁇ 40 ° C, time 32-36 hours.
  • the lignin reaction is degraded under the conditions of biological bacteria, and its specific effect is exerted.
  • Fine refining The coarse slurry discharged from the above is then transferred to a high-concentration refiner for fine refining to disperse the fiber bundle into a single fiber.
  • Slurry screening After two stages of refining, the slurry contains a small number of fiber bundles, which are screened, filtered, and re-slurry to reach a single fiber.
  • Submersible, washing The pulp after coarse refining and fine refining is mechanically rubbed, most of which is bent and twisted. It is soaked in warm water to eliminate the fiber deflection caused by refining and stretch it.
  • Paperboard The paper pulp prepared above is fed into a paper machine, and paperboard is produced by a papermaking process.
  • the caragana peels separated in A and 3 are rich in nutrients and fermented into cattle and sheep feed.
  • the liquid After immersing and washing the liquid in B and 4, the liquid is turbid, and after flocculation and sedimentation, the supernatant is recovered and reused.
  • the sediment is input into the biogas tank to produce biogas, which is fed into the gas dual-purpose boiler for fuel, which can save energy. Consumption.
  • Bio-organic fertilizer The biogas residue and biogas slurry fermented by the biogas tank are rich in bio-organic fertilizer.
  • the liquid is used as crop topdressing and flower nutrient solution, and solid granulation is used as base fertilizer, which is green fertilizer.
  • the biological bacterial liquid pulping method when using herbal raw materials is specifically described.
  • the biopulping of the remaining herbal materials, such as wheat straw, straw, and corn stalk, can be carried out with reference to the process.
  • the composite flora is configured according to the following mass ratio:
  • the composite flora is configured according to the following mass ratio:
  • the composite flora is configured according to the following mass ratio:
  • the process of the biological bacterial slurry method is divided into three stages: preparation stage, pulping section and by-product section.

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
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Abstract

L'invention concerne une solution bactérienne biologique, une application de celle-ci, et un procédé d'application correspondant dans la préparation d'une fibre textile, d'une cellulose destinée à être utilisée en tant qu'additif, d'une pâte de solution bactérienne biologique. La flore microbienne complexe comprend Rheinheimera tangshanensis déposée sous le numéro CGMCC No 5972, Pseudomonas fluorescens déposée sous le numéro CGMCC No 5974 et Wickerhamomyces anomalus déposée sous le numéro CGMCC No 5975. Le procédé comprend: la formulation d'une solution bactérienne, le traitement des matières premières, et la préparation ou la trituration de la fibre.
PCT/CN2013/001083 2012-09-17 2013-09-16 Solution bactérienne biologique et son application WO2014040382A1 (fr)

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CA2885474A CA2885474A1 (fr) 2012-09-17 2013-09-16 Solution bacterienne biologique et son application

Applications Claiming Priority (6)

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CN201210342545.1 2012-09-17
CN201210342165.8 2012-09-17
CN201210342705.2 2012-09-17
CN2012103421658A CN103074214A (zh) 2012-09-17 2012-09-17 一种复合菌群及其在制备纺织纤维中的应用
CN201210342705.2A CN103074257B (zh) 2012-09-17 2012-09-17 一种复合菌群及其生物菌液制浆法
CN201210342545.1A CN103074252B (zh) 2012-09-17 2012-09-17 一种复合菌群及其在制备添加剂用纤维素中的应用

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Citations (5)

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CN102002468A (zh) * 2010-08-13 2011-04-06 东华大学 一种荧光假单胞菌da4菌株及其获得方法和应用
CN103074257A (zh) * 2012-09-17 2013-05-01 贾平 一种复合菌群及其生物菌液制浆法
CN103074252A (zh) * 2012-09-17 2013-05-01 贾平 一种复合菌群及其在制备添加剂用纤维素中的应用
CN103074214A (zh) * 2012-09-17 2013-05-01 贾平 一种复合菌群及其在制备纺织纤维中的应用

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CN102002468A (zh) * 2010-08-13 2011-04-06 东华大学 一种荧光假单胞菌da4菌株及其获得方法和应用
CN103074257A (zh) * 2012-09-17 2013-05-01 贾平 一种复合菌群及其生物菌液制浆法
CN103074252A (zh) * 2012-09-17 2013-05-01 贾平 一种复合菌群及其在制备添加剂用纤维素中的应用
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ZHANG, XIAOXIA ET AL.: "Studies on Polyphasic Taxonomy of Rice Root-associated Bacteria and Description of Rheinheimera Tangshanensis sp. nov.", AGRICULTURE, CHINA DOCTORAL DISSERTATIONS FULL-TEXT DATABASE, vol. 10, 2009, pages D043-4 *

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