WO2014019254A1

WO2014019254A1 - Vacuum blood collection pipe capable of directly separating plasma and method thereof

Info

Publication number: WO2014019254A1
Application number: PCT/CN2012/079961
Authority: WO
Inventors: 朱德新
Original assignee: 上海科华检验医学产品有限公司
Priority date: 2012-07-31
Filing date: 2012-08-10
Publication date: 2014-02-06
Also published as: WO2014019255A1

Abstract

A vacuum blood collection pipe capable of directly separating plasma and a preparation method thereof. The vacuum blood collection pipe comprises a blood collection pipe body (1), a blood collection inner pipe (6), a rubber plug (9), and a filter element (4). The blood collection inner pipe (6) is disposed in the blood collection pipe body (1), and the rubber plug (9) is disposed at the top of the blood collection pipe body (1). The structure of the rubber plug (9) is similar to a plug shape. A semicircular groove is disposed in the center of the upper portion in the structure of the rubber plug (9). Left and right ends at the lower portion of the rubber plug (9) are embedded into the blood collection pipe body (1) and connected to the blood collection inner pipe (6). A plasma outlet (10) is disposed in the middle of the bottom of the blood collection inner pipe (6), and a lower portion of the plasma outlet (10) is a plasma collection chamber (2). A filtering film (3) is disposed at the upper portion of the plasma outlet (10) in the blood collection inner pipe (6). The filter element (4) is disposed at the upper portion of the filtering film (3). A buffer plate (5) is further disposed on the filter element (4). Therefore, a blood collection chamber (8) is formed in the blood collection inner pipe (6), and a layer of anticoagulants (7) is disposed on an inner wall of the blood collection inner pipe (6). According to the present invention, by using a difference between physical forms of a visible component and a plasma composite of blood, the plasma can be rapidly separated, having the features of simpleness and convenience, safety and rapidness.

Description

Vacuum blood collection tube capable of directly separating plasma and method thereof

[Technical Field] The present invention relates to the field of clinical medical device technology, and in particular to a vacuum blood collection tube capable of directly separating plasma and a method thereof.

[Background Art] The detection of blood specimens is a conventional technical means for clinical examination of medical institutions, and blood specimens for in vitro diagnosis are mainly divided into arterial blood and venous blood, clinically, arterial blood specimens are mainly used for blood gas index analysis, and venous blood specimens. The detection range is widely used, such as for detecting biochemical indicators, hemagglutination indicators, blood rheology indicators, routine blood analysis, trace element and ionic composition analysis, nutrient detection, drug concentration detection, genetic testing, infectious disease pathogens. Learning, microbiological testing, etc. Due to the differences in the detection instruments used in different detection indicators, venous blood specimens are classified into plasma, serum and whole blood according to their uses. The plasma specimens have traditionally been obtained mainly by centrifugation. After the blood is ex vivo, the anticoagulant is treated by anticoagulant, and the plasma specimen is separated by a centrifuge under a certain centrifugal force. The vacuum blood collection tube commonly used in the prior art is composed of a blood collection tube body, a rubber stopper and a head cover, an additive, a label, etc., and does not have the function of directly separating plasma. If it is necessary to separate plasma, it must be centrifuged after blood collection. To complete, the anticoagulated blood needs to be centrifuged to separate the plasma. On the one hand, if the centrifugation conditions are not well controlled, hemolysis may occur, or the collection container may be damaged during the centrifugation process, resulting in leakage or complete damage of the specimen; In the process of centrifugation and after separation, the plasma and other components of the blood are mixed together. Under the action of centrifugal force or during storage, the substances in the red blood cells are released into the plasma, which affects the final test result. , not safe and reliable. There is also a vacuum blood collection tube (publication number CN 202173399 U) which can directly separate plasma, and the blood is quantitatively absorbed by the adsorption material built in the sampling component, and then added into the blood sample storage container containing the liquid anticoagulant, fully well mixed. Inserting the plasma separation component equipped with the filter element into the blood sample preservation container, the top of the plasma separation component is a hollow elastic piston, and the outer part of the piston forms a slightly sealed closed cavity with the inner wall of the blood sample preservation container, and the hollow piston is provided with a filter element inside. Through the top-down movement of the piston, when the piston passes the collected anticoagulant sample, due to the pressure, the plasma portion of the anticoagulant can pass through the filter element and is in the upper part of the filter element, and the blood that cannot pass through the filter element is formed. It is still in the lower part of the filter element. However, the operation method of the vacuum collecting tube is complicated, and the operation technology of the operator is required in the process of collecting the plasma sample, and the time spent in the collecting process is also long, the efficiency is not high, and it is not practical enough.

SUMMARY OF THE INVENTION The present invention is to solve the defects and defects in the prior art that the blood collection container cannot directly separate plasma, and provides a vacuum blood collection tube and a method thereof, which can directly separate plasma, which is novel in structure, safe and reliable. In order to achieve the above object, a vacuum blood collection tube capable of directly separating plasma is provided, which comprises a blood collection tube body, an inner tube, a rubber stopper and a filter element, wherein the top of the blood collection tube body is provided with a rubber stopper and a rubber stopper. The structure is approximately plug shape, and the upper center of the rubber plug structure is provided with a semi-circular groove, and the left and right ends of the rubber plug are embedded and connected with the blood collection tube and the inner tube of the blood collection tube, and the inner tube of the blood collection tube is used. a plasma outlet is arranged in the middle of the bottom, a plasma collection chamber is arranged in the lower part of the plasma outlet, a filter membrane is arranged on the upper part of the plasma outlet of the blood collection tube, a filter element is arranged on the upper part of the filtration membrane, and a buffer sheet is arranged on the filter element. Thus, a blood collection chamber is formed in the inner tube of the blood collection tube from the upper portion of the filter element to the top of the inner tube of the blood collection tube, and an anticoagulant is disposed on the inner wall of the inner tube of the blood collection tube, and the anticoagulant includes heparin sodium, lithium heparin, and lemon. Sodium, dipotassium edetate, tripotassium edetate, disodium edetate, oxalic acid Potassium, sodium oxalate, hirudin and stabilizers for target analytes. The blood collection tube body is made of one or more of glass, polyester plastic, and polymer material. The inner tube of the blood collection tube is mainly made of a polymer material, including high density polyethylene, low density polyethylene, polypropylene, polystyrene, polycarbonate, polyethylene, terephthalate. The filter core is made of ultrafine plant fiber, synthetic fiber, polyester sintered product, filter paper, preferably polypropylene synthetic fiber, polyethylene synthetic fiber, polyethylene terephthalate synthetic fiber, carboxymethyl fiber. , microcrystalline cellulose, processed plant fibers. The material of the rubber stopper includes natural rubber, halogenated butyl rubber, synthetic rubber, and thermoplastic elastomer. A buffer sheet is disposed above the filter element, and the buffer material of the buffer sheet comprises a lyophilized anticoagulant tablet, a nylon membrane, a filter paper, a synthetic cellulose membrane, and a buffered anticoagulant is further disposed on the buffer sheet. The filter membrane is a microporous membrane, and the manufacturing materials thereof include cellulose acetate, nitrocellulose, mixed cellulose ester, nylon 6, nylon 66, polyvinylidene fluoride, polytetrafluoroethylene, polyethersulfone, polypropylene. material. A method for directly separating plasma vacuum blood collection tubes, characterized in that the method utilizes a different physical form of plasma and blood formation, and an inner tube equipped with a filter element is installed inside the blood collection tube to separate the blood collection tube into blood collection. The chamber and the plasma collection chamber, through the anticoagulant and the filter element built into the inner tube, the blood flowing into the blood collection chamber will not solidify, and the formed part of the blood is blocked by the filter element in the upper part thereof, that is, the blood collection chamber, and the plasma component can pass smoothly. The filter element flows into the lower part of the blood collection tube, ie the plasma collection chamber, thereby allowing plasma to The formation of the blood is separated, and the blood collection gel plug connected to the upper end of the inner tube is discarded. The entire inner tube can be used to use the plasma sample of the plasma collection chamber for various tests; the filter membrane, the filter element and the filter tube are sequentially loaded in the inner tube. Spraying the filter paper buffer sheet with anticoagulant, spraying the anticoagulant on the inner tube wall, loading the prepared inner tube into the blood collection tube body, placing the rubber plug on the upper part of the blood collection tube body, and placing it in the vacuum machine After the vacuum is placed, the plug is pressed. The blood collection tube body is made of borate glass, the blood collection tube rubber is made of chlorinated butyl rubber, and the blood collection tube is made of high density polyethylene, and the filtration membrane is an asymmetric polysulfone filtration membrane. The filter element is a polyethylene terephthalate fiber filter, the buffer sheet is a filter paper buffer sheet sprayed with an anticoagulant of ethylenediaminetetraacetate, and the inner tube wall is sprayed with ethylenediamine four. Dipotassium acetate anticoagulant. The invention has the advantages of utilizing the physical form difference of blood forming component and plasma component, and rapidly separating plasma by the principle of simple filtering method, and has the characteristics of simple, safe and rapid, and can be directly separated and qualified without using a conventional centrifugal machine. Plasma for clinical testing. The material used is not only made into a transparent tube body but also can effectively ensure the negative pressure of the vacuum blood collection tube, the material is easy to obtain, the cost is low, the effect is good, economical and practical, safe and reliable.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic view showing the main structure of a vacuum blood collection tube of the present invention;

Figure 2 is a partial enlarged view of a portion of Figure 1 of the present invention;

As shown in the figure, in the figure: 1. Blood collection tube 2. Plasma collection chamber 3. Filter membrane 4. Filter element 5. Buffer sheet 6. Blood collection tube 7. Anticoagulant 8. Blood collection chamber 9. Rubber stopper 10. Plasma outflow;

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a summary drawing of the present invention.

[detailed description]

The present invention will be further described below in conjunction with the accompanying drawings. The structure and principle of such a device are People are very clear. It is understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

The invention provides a vacuum blood collection tube capable of directly separating plasma and a method thereof, which can obtain qualified plasma without using other equipment, and the principle is that the physical form of plasma and blood formation is different, and the blood vessel is installed inside the blood collection tube. An inner tube equipped with a filter element separates the inside of the blood collection tube into a blood collection chamber and a plasma collection chamber. The anticoagulant and the filter element built into the inner tube prevent the blood flowing into the blood collection chamber from coagulating, and the blood is formed into a filter element. Blocked in the upper part, that is, the blood collection chamber, and the plasma component can smoothly pass through the filter element into the lower part of the blood collection tube, that is, the plasma collection chamber, thereby separating the plasma from the blood formation, and pulling out the blood collection tube plug connected to the upper end of the inner tube. Plasma samples from the plasma collection chamber can be used for various tests by discarding the entire inner tube.

The tube body is made of glass, plastic or other polymer material. It is a round bottom or a pointed bottom, flat bottom tube structure with one end closed and the other end open. The inner part of the blood collection tube body is filled with an inner tube filled with a filter element. The inner tube is made of plastic or other polymer material, and one end is open to the upper opening and is connected with the rubber plug, the other end is hollow τ-shaped structure, and the lower part is opened with a plasma flow outlet; The outer diameter of the inner tube is slightly smaller than the inner diameter of the blood vessel tube body, and is closely adhered to the inner wall of the blood collection tube after assembly; the filter element is made of plant fiber, synthetic fiber or polyester sintered material, filter paper material, and the pore diameter is smaller than the blood forming component. Only the plasma can be filtered and cannot be formed by other blood; the inner wall of the inner tube is coated with an anticoagulant, and the anticoagulant can also be sprayed on the other material on the upper part of the filter element; the upper part of the filter element can be placed with a buffer material as an anti-coagulant The coagulant carrier, the filter element can be placed on the lower part of the filter element, and the liquid flowing out of the filter element is further filtered to ensure the quality of the plasma.

The blood collection rubber plug is made of elastic material, which can completely seal the open end of the blood collection tube body, ensure that a certain negative pressure can be preset inside the vacuum blood collection tube, and maintain the vacuum degree inside the tube for a certain period of time. The crown of the rubber plug is bulged or the safety head cover is placed outside, and is easily pulled out after use. The tube cover has a circular groove in the middle to facilitate the puncture of the blood collection needle during blood collection; the rubber plug has two contact parts with the tube body. Level, upper level The outer diameter matches the inner diameter of the blood vessel tube to ensure the vacuum inside the tube. The outer diameter of the next layer matches the inner diameter of the inner tube, and there is a certain interference. It can ensure that the rubber plug together with the whole inner tube after the plasma is filtered out. The blood vessel tube is pulled out.

The material for manufacturing the blood vessel tube body includes silicate glass, borate glass, soda lime glass, polyester plastic material, polymer material, preferably borate glass, polyethylene terephthalate, polyethylene glycol. Modified polyethylene terephthalate, polycarbonate, polyethylene naphthalate or polyethylene terephthalate and polyethylene naphthalate composite. Because the above materials are not only made into a transparent tube body but also can effectively ensure the vacuum pressure of the vacuum blood collection tube, the material is easy to obtain and the cost is relatively low.

Materials for manufacturing the inner tube body include high density polyethylene, low density polyethylene, polypropylene, polystyrene, polycarbonate, polyethylene terephthalate or other high molecular materials, preferably high density polyethylene, The inner tube is relatively transparent and soft, and can be attached to the inner wall of the blood collection tube on the one hand, and easily removed from the blood collection tube after plasma filtration on the other hand.

Anticoagulant sprayed on the inner wall of the inner tube includes sodium heparin, lithium heparin, sodium citrate, dipotassium edetate, tripotassium edetate, disodium edetate, potassium oxalate, sodium oxalate, leeches In order to adapt to different testing items, such as blood glucose testing, stabilizers such as sodium fluoride and sodium iodide may be added as long as they do not affect the subsequent detection of plasma.

The material for manufacturing the filter element includes ultrafine plant fiber, synthetic fiber, polyester sintered product, filter paper, preferably polypropylene synthetic fiber, polyethylene synthetic fiber, polyethylene terephthalate synthetic fiber, carboxymethyl cellulose, micro Crystalline cellulose, processed plant fibers, such as defatted cotton, the pore size of the ultrafine fibers is preferably a micron-sized product, preferably a polyethylene terephthalate synthetic fiber, which does not form hemolysis and does not bind to blood. Target detection component.

Materials for manufacturing vascular rubber plugs include natural rubber, halogenated butyl rubber, synthetic rubber, thermoplastic elastomer, preferably halogenated butyl rubber, which is not only easy to mold, but also has sufficient elasticity to ensure effective The preset vacuum of the blood collection tube is also easy to pull out after use.

A cushioning material may be attached to the filter element, and the material is coated with an anticoagulant or an additive, so that the blood flowing into the blood collection tube first can be quickly mixed with the anticoagulant and does not solidify. The cushioning material comprises a tablet of a freeze-dried anticoagulant, a nylon membrane, a filter paper, a synthetic cellulose membrane, etc., preferably a filter paper sheet coated with an anticoagulant, and the buffer material prevents the blood from being in contact with the spray when initially flowing into the blood collection chamber. The anticoagulant on the tube wall directly flows into the filter to form a solid, forming a tiny clot, which causes the separated plasma to fail.

A filter membrane can be added under the filter element. The filter membrane has a circular sheet shape, and the outer diameter is equal to the inner diameter of the inner tube. It is made of microporous inert hydrophobic material, and the pore diameter is smaller than the blood cell component, which can effectively block the formation of blood. Does not itself react with any components of the blood, nor does it adsorb any components of the blood. Such materials include microporous membranes made of materials such as cellulose acetate, nitrocellulose, mixed cellulose ester, nylon 6, nylon 66, polyvinylidene fluoride, polytetrafluoroethylene, polyethersulfone, polypropylene, and the like. An asymmetric polysulfone membrane is preferred, and the asymmetric structure of the membrane captures cellular components in the blood without cleavage, does not form hemolysis, and does not adsorb critical biomarkers. There should be sufficient space between the upper part of the filter element and the lower end of the rubber plug, otherwise it is difficult to obtain a sufficient amount of blood. There should also be sufficient space between the lower part of the filter element and the bottom of the blood collection tube, otherwise the filtered plasma cannot be accommodated.

The invention can realize the direct separation of qualified blood plasma without using a centrifuge after blood collection by the above design.

Example 1

The blood vessel tube is made of borate glass, the vascular rubber plug is made by chlorinated butyl rubber, the blood collection tube is made of high density polyethylene, and the inner tube is sequentially filled with asymmetric polysulfone filter membrane and polyethylene terephthalate. a fiber filter core and a filter paper buffer sheet sprayed with an anticoagulant of ethylenediaminetetraacetic acid dipotassium, and an anti-coagulant of ethylenediaminetetraacetate is sprayed on the inner tube wall, and the prepared inner tube is loaded into the blood collection tube body. Place the rubber stopper on top of the blood collection tube body, put it into the vacuum machine and preset the vacuum and then press it. Example 2

The vascular tube body is made of polyethylene terephthalate, the vascular rubber plug is made of chlorinated butyl rubber, the blood collection tube is made of high density polyethylene, and the inner tube is loaded with asymmetric polysulfone filter membrane and polyethylene benzene. a diester fiber filter element and a filter paper buffer sheet coated with heparin sodium, a heparin sodium anticoagulant sprayed on the inner tube wall, the inner tube prepared above is loaded into the blood collection tube body, and the rubber stopper is placed on the blood collection tube body Above, put it into the vacuum machine and preset the vacuum and then press it.

Example 3

The vascular tube body is made of polyethylene glycol modified polyethylene terephthalate, the vascular rubber plug is made by chlorinated butyl rubber, the blood collection tube is made of high density polyethylene, and the inner tube is sequentially filled with asymmetric polysulfone filtration. Membrane, polyethylene terephthalate fiber filter and filter paper buffer coated with lithium heparin, sprayed with heparin lithium anticoagulant on the inner tube wall, the inner tube prepared above is loaded into the blood collection tube body, and the rubber stopper is inserted Place it on top of the blood collection tube body, put it into the vacuum machine and preset the vacuum and then press it.

Example 4

The vascular tube body is made of polyethylene terephthalate, the vascular rubber plug is made of bromobutyl rubber, the inner tube of the blood collection tube is made of polypropylene, and the inner tube is sequentially filled with asymmetric polysulfone filter membrane and polyethylene terephthalic acid. The acid ester fiber filter element and the filter paper buffer paper coated with the tripotassium EDTA, the inner tube wall is sprayed with the anti-coagulant of ethylenediaminetetraacetic acid, and the prepared inner tube is loaded into the blood collection tube body. Place the rubber stopper on top of the blood collection tube body, put it into the vacuum machine and preset the vacuum and then press it.

Example 5

The blood vessel tube is made of borate glass, the vascular rubber plug is made of bromobutyl rubber, the blood collection tube is made of high transparent polypropylene, and the inner tube is sequentially filled with asymmetric polysulfone filter membrane and polyethylene terephthalate. a fiber filter and a filter paper coated with sodium citrate anticoagulant, spraying a sodium citrate anticoagulant on the inner tube wall, loading the prepared inner tube into the blood collection tube body, and placing the rubber plug on the blood collection tube body Above, put vacuum After the vacuum is preset in the machine, the plug is pressed.

Example 6

Polyethylene terephthalate modified polyethylene terephthalate is used to make blood vessel tube, halogenated butyl rubber is used to make blood vessel rubber plug, low density polyethylene is used to make blood collection tube, and inner tube is loaded with asymmetric polysulfone filtration. a buffer sheet made of a film, a polyethylene terephthalate fiber filter and a polyester film coated with a hirudin anticoagulant, and a hydroquinone anticoagulant is sprayed on the inner tube wall, and the prepared inner tube is loaded into the tube. In the vascular tube body, the rubber stopper is placed above the blood collection tube body, and placed in a vacuum machine to preset a vacuum and then tamped.

Example 7

The valve tube is made of polyethylene terephthalate, the vascular rubber plug is made of halogenated butyl rubber, the inner tube of blood collection tube is made of high density polyethylene, and the inner tube is loaded with asymmetric polysulfone filter membrane and polyethylene terephthalate. a buffer sheet made of a polyesterate fiber filter and a polyester film coated with potassium oxalate anticoagulant and sodium fluoride stabilizer, sprayed with potassium oxalate anticoagulant and sodium fluoride stabilizer on the inner tube wall, which is prepared as described above. The inner tube is loaded into the blood collection tube body, and the rubber stopper is placed above the blood collection tube body, and placed in a vacuum machine to preset a vacuum and then pressed.

According to the above examples, the vacuum blood collection tube was prepared, and the vein was punctured with a venous puncture needle, and blood was collected separately. After the blood collection, the blood was placed upright for 15 minutes, and the blood collection rubber plug and the inner tube were discarded. Observe that the blood collected in the blood collection tube is a pale yellow, transparent or translucent liquid.

Claims

A vacuum blood collection tube capable of directly separating plasma, comprising a blood collection tube body, an inner tube, a rubber stopper, and a filter element, wherein the blood collection tube body (1) is provided with a rubber stopper (9) at the top thereof. The structure of the rubber plug (9) is approximately plug shape, and the upper center of the rubber plug structure is provided with a semi-circular groove, and the left and right ends of the rubber plug (9) are embedded and connected with the blood collection tube body (1) and the blood collection tube body ( 1) in the endovascular tube (6), in the middle of the bottom of the blood collection tube (6) is provided with a plasma outflow port (10), the lower part of the plasma outflow port is a plasma collection chamber (2), in the endovascular tube (6) plasma The upper part of the outflow port is provided with a filter membrane (3), the upper part of the filter membrane (3) is provided with a filter element (4), and the filter element (4) is further provided with a buffer sheet (5), thereby being in the inner tube of the blood collection tube ( 6) The blood collection chamber (8) between the upper part of the filter element (4) and the top of the inner tube (6) of the blood collection tube is provided, and an anticoagulant (7) is arranged on the inner wall of the inner tube (6) of the blood collection tube, Anticoagulants include sodium heparin, lithium heparin, sodium citrate, dipotassium edetate, B Amine tripotassium EDTA, disodium edetate, potassium oxalate, sodium oxalate, hirudin and stabilizer detection target object.

2. A vacuum blood collection tube capable of directly separating plasma according to claim 1, wherein said blood collection tube body (1) is one or one of glass, polyester plastic, and polymer material. More than one kind of compound.

3. A vacuum blood collection tube capable of directly separating plasma according to claim 1, wherein said blood collection tube (6) is made of a polymer material, including high density polyethylene. , low density polyethylene, polypropylene, polystyrene, polycarbonate, polyethylene, terephthalate.

4. A vacuum blood collection tube capable of directly separating plasma according to claim 1, wherein said filter element (4) is made of ultrafine plant fibers, synthetic fibers, polyester sintered products, filter paper. Preferred are polypropylene synthetic fibers, polyethylene synthetic fibers, polyethylene terephthalate synthetic fibers, carboxymethyl cellulose, microcrystalline cellulose, and processed plant fibers.

A vacuum blood collection tube capable of directly separating plasma according to claim 1, wherein the material of the rubber stopper (9) comprises natural rubber, halogenated butyl rubber, synthetic rubber, and thermoplastic elastomer.

6. A vacuum blood collection tube capable of directly separating plasma according to claim 1, wherein said filter element (4) is provided with a buffer sheet (5), and the buffer material of the buffer sheet comprises lyophilized Anticoagulant tablet, nylon membrane, filter paper, synthetic cellulose membrane, and a coated anticoagulant on the buffer sheet.

7. A vacuum blood collection tube capable of directly separating plasma according to claim 1, wherein said filtration membrane (3) is a microporous membrane, and the material for which the preparation comprises cellulose acetate, nitrocellulose, Mixed cellulose ester, nylon 6, nylon 66, polyvinylidene fluoride, polytetrafluoroethylene, polyethersulfone, polypropylene material.

8. A method for directly separating plasma vacuum blood collection tubes, characterized in that the method utilizes a different physical form of plasma and blood formation, and an inner tube equipped with a filter element is installed inside the blood collection tube to separate the blood collection tube interior The blood collection chamber and the plasma collection chamber pass through the anticoagulant and the filter element built in the inner tube, so that the blood flowing into the blood collection chamber does not solidify, and the formed part of the blood is blocked by the filter element in the upper part thereof, that is, the blood collection chamber, and the plasma component can be Smoothly through the filter element into the lower part of the blood collection tube, that is, the plasma collection chamber, so that the plasma and blood formation are separated. Pull out the blood collection tube plug connected to the upper end of the inner tube and discard the entire inner tube to use the plasma of the plasma collection chamber. Specimens are used for various tests; the filter membrane, the filter element and the filter paper buffer sheet sprayed with anticoagulant are sequentially loaded into the inner tube, the anti-coagulant is sprayed on the inner tube wall, and the prepared inner tube is loaded into the blood collection tube body. Place the rubber stopper on top of the blood collection tube body, put it into the vacuum machine and preset the vacuum and then press it.

9. The method of claim 8, wherein the blood collection tube body is made of borate glass, and the blood collection tube is made of chlorinated butyl rubber. , The blood collection tube is made of high-density polyethylene, the filter membrane is an asymmetric polysulfone filter membrane, the filter element is a polyethylene terephthalate fiber filter element, and the buffer sheet is sprayed with ethylene diamine. A filter paper buffer sheet of the acetic acid secondary anticoagulant, and the inner tube wall is sprayed with an anti-coagulant of ethylenediaminetetraacetate.