WO2013143508A3 - Método para la detección, recuperación, identificación y enumeración simultánea de microorganismos y dispositivos para su ejecución - Google Patents

Método para la detección, recuperación, identificación y enumeración simultánea de microorganismos y dispositivos para su ejecución Download PDF

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Publication number
WO2013143508A3
WO2013143508A3 PCT/CU2013/000002 CU2013000002W WO2013143508A3 WO 2013143508 A3 WO2013143508 A3 WO 2013143508A3 CU 2013000002 W CU2013000002 W CU 2013000002W WO 2013143508 A3 WO2013143508 A3 WO 2013143508A3
Authority
WO
WIPO (PCT)
Prior art keywords
counting
recovery
identification
devices
simultaneous detection
Prior art date
Application number
PCT/CU2013/000002
Other languages
English (en)
French (fr)
Other versions
WO2013143508A2 (es
Inventor
Claudio Rodríguez Martínez
Jesús Eduardo GONZÁLEZ RUIZ
Tamara LOBAINA RODRÍGUEZ
Raisa Zhurbenko
Ana Iris BRITO GONZÁLEZ
Mónica LÓPEZ HERNÁNDEZ
Javier ARAGÓN FERNÁNDEZ
Ivonne ALFONSO VALDÉS
Adelaida ORTEGA SURÍS
Original Assignee
Centro Nacional De Biopreparados (Biocen)
Centro Nacional de Investigaciones Científicas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centro Nacional De Biopreparados (Biocen), Centro Nacional de Investigaciones Científicas filed Critical Centro Nacional De Biopreparados (Biocen)
Priority to US14/389,568 priority Critical patent/US20150148258A1/en
Priority to EP13767434.7A priority patent/EP2837690B1/en
Priority to MX2014011804A priority patent/MX2014011804A/es
Priority to BR112014024361-1A priority patent/BR112014024361B1/pt
Priority to CA2883197A priority patent/CA2883197C/en
Priority to ES13767434.7T priority patent/ES2650453T3/es
Priority to RU2014143767A priority patent/RU2644683C2/ru
Publication of WO2013143508A2 publication Critical patent/WO2013143508A2/es
Publication of WO2013143508A3 publication Critical patent/WO2013143508A3/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/14—Streptococcus; Staphylococcus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/21—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Pseudomonadaceae (F)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/245—Escherichia (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/315—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Streptococcus (G), e.g. Enterococci
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/37—Assays involving biological materials from specific organisms or of a specific nature from fungi
    • G01N2333/38—Assays involving biological materials from specific organisms or of a specific nature from fungi from Aspergillus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/37—Assays involving biological materials from specific organisms or of a specific nature from fungi
    • G01N2333/39—Assays involving biological materials from specific organisms or of a specific nature from fungi from yeasts
    • G01N2333/40—Assays involving biological materials from specific organisms or of a specific nature from fungi from yeasts from Candida

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La presente invención describe un método y dispositivos para la detección, recuperación, identificación y enumeración simultánea de una pluralidad de microorganismos consistente en proveer mezclas de sustancias nutritivas especialmente seleccionadas entre aquellas que acortan la fase lag de crecimiento de bacterias y mohos y que, conjuntamente con marcadores enzimáticos fluorescentes, cromogénicos o bioluminiscentes y otros componentes nutritivos o inhibidores del crecimiento, son embebidos en estructuras tridimensionales de arcillas o cerámicas naturales o artificiales con cavidades de diferentes dimensiones y formas y superficies específicas entre 2 x 103 y 6 x 108 m2/m3.
PCT/CU2013/000002 2012-03-30 2013-03-27 Método para la detección, recuperación, identificación y enumeración simultánea de microorganismos y dispositivos para su ejecución WO2013143508A2 (es)

Priority Applications (7)

Application Number Priority Date Filing Date Title
US14/389,568 US20150148258A1 (en) 2012-03-30 2013-03-27 Method for simultaneous detection, recovery, identification and counting of microorganisms and devices for the implementation of said method
EP13767434.7A EP2837690B1 (en) 2012-03-30 2013-03-27 Method for simultaneous detection, recovery, identification and counting of microorganisms and three dimentioanl structure for the implementation of said method
MX2014011804A MX2014011804A (es) 2012-03-30 2013-03-27 Metodo para la deteccion, recuperacion, identificacion y enumeracion simultanea de microorganismos y dispositivos para su ejecucion.
BR112014024361-1A BR112014024361B1 (pt) 2012-03-30 2013-03-27 Método para a detecção, a recuperação, a identificação e a contagem simultâneas de micro-organismos e dispositivos para a sua implementação
CA2883197A CA2883197C (en) 2012-03-30 2013-03-27 Method for simultaneous detection, recovery, identification and counting of microorganisms and devices for the implementation of said method
ES13767434.7T ES2650453T3 (es) 2012-03-30 2013-03-27 Método para la detección, recuperación, identificación y conteo simultáneos de bacterias y hongos, y disposición de estructura tridimensional para la implementación de dicho método
RU2014143767A RU2644683C2 (ru) 2012-03-30 2013-03-27 Способ получения трехмерных структур, используемых для детекции, выделения или подсчета микроорганизмов

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CU20120055A CU24137B1 (es) 2012-03-30 2012-03-30 Método para la detección, recuperación, identificación y enumeración simultánea de microorganismos
CUCU/P/2012/0055 2012-03-30

Publications (2)

Publication Number Publication Date
WO2013143508A2 WO2013143508A2 (es) 2013-10-03
WO2013143508A3 true WO2013143508A3 (es) 2014-05-08

Family

ID=49261336

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CU2013/000002 WO2013143508A2 (es) 2012-03-30 2013-03-27 Método para la detección, recuperación, identificación y enumeración simultánea de microorganismos y dispositivos para su ejecución

Country Status (9)

Country Link
US (1) US20150148258A1 (es)
EP (1) EP2837690B1 (es)
BR (1) BR112014024361B1 (es)
CA (1) CA2883197C (es)
CU (1) CU24137B1 (es)
ES (1) ES2650453T3 (es)
MX (1) MX2014011804A (es)
RU (1) RU2644683C2 (es)
WO (1) WO2013143508A2 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
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JP6722687B2 (ja) 2014-11-25 2020-07-15 スリーエム イノベイティブ プロパティズ カンパニー 微生物を増殖又は貯蔵するためのデバイス及びキット、並びにその製造及び使用方法
RU2636620C1 (ru) * 2016-10-25 2017-11-24 Федеральное государственное бюджетное учреждение науки "Тюменский научный центр Сибирского отделения РАН" (ТюмНЦ СО РАН) Способ оптической оценки концентрации микробных клеток в суспензии
CN109182445B (zh) * 2018-09-20 2021-10-22 吉林农业大学 一种霉菌和酵母计数测试片、制备方法及其应用
WO2020090859A1 (ja) 2018-10-30 2020-05-07 国立大学法人名古屋大学 細胞外小胞を捕捉するために用いられるデバイス、細胞外小胞の保存方法および移送方法
CN110257468B (zh) * 2019-06-03 2022-05-27 北京鑫骥金诺医疗器械有限公司 一种用于细菌计数的质控的组合物、制备方法及其应用
CN112795486B (zh) * 2020-12-30 2023-09-19 兴源环境科技股份有限公司 一种畜禽粪污微藻养殖同步产乙醇的方法

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RU2139344C1 (ru) * 1997-12-23 1999-10-10 Научно-исследовательский институт эпидемиологии и микробиологии СО РАМН Способ приготовления питательной среды для культивирования сальмонелл
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Also Published As

Publication number Publication date
EP2837690B1 (en) 2017-09-13
BR112014024361A2 (pt) 2017-07-25
US20150148258A1 (en) 2015-05-28
BR112014024361A8 (pt) 2022-03-08
WO2013143508A2 (es) 2013-10-03
CU24137B1 (es) 2015-12-23
EP2837690A2 (en) 2015-02-18
CA2883197A1 (en) 2013-10-03
RU2014143767A (ru) 2016-05-27
ES2650453T3 (es) 2018-01-18
BR112014024361B1 (pt) 2022-06-07
EP2837690A4 (en) 2015-11-25
CA2883197C (en) 2020-03-31
MX2014011804A (es) 2015-03-19
CU20120055A7 (es) 2013-12-27
RU2644683C2 (ru) 2018-02-13

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