WO2013120081A1 - 1h- pyrrolo [2, 3 -b] pyridine derivatives and their use as radical quenchers - Google Patents

1h- pyrrolo [2, 3 -b] pyridine derivatives and their use as radical quenchers Download PDF

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WO2013120081A1
WO2013120081A1 PCT/US2013/025590 US2013025590W WO2013120081A1 WO 2013120081 A1 WO2013120081 A1 WO 2013120081A1 US 2013025590 W US2013025590 W US 2013025590W WO 2013120081 A1 WO2013120081 A1 WO 2013120081A1
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alkyl
hydrogen
halo
compound according
halogen
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PCT/US2013/025590
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English (en)
French (fr)
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Sidney Hecht
Xiaoqing Cai
Omar Khdour
Jose I. ARMENDARIZ GUAJARDO
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Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State University
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Priority to US14/371,579 priority Critical patent/US9440967B2/en
Publication of WO2013120081A1 publication Critical patent/WO2013120081A1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
    • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
    • C07D471/04—Ortho-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants

Definitions

  • the present disclosure provides biologically active compounds multifunctional radical quenchers of formula (I) and pharmaceutically acceptable salts thereof, compositions comprising these compounds, and methods of using these compounds in a variety of applications, such as treatment or suppression of diseases associated with decreased mitochondrial function resulting in diminished ATP production and/or oxidative stress and/or lipid peroxidation. , . ' ⁇ .
  • Mitochondria are intracellular organelles responsible for a number of metabolic transformations and regulatory functions. They produce much of the ATP employed by eukaryotic cells. They are also the major source of free radicals and reactive oxygen species that cause oxidative stress. Consequently, mitochondrial defects are damaging, particularly to neural and muscle tissues which have high energy level demands. Thus, energetic defects have been implicated in forms of movement disorders, cardiomyopathy, myopathy, blindness, and deafness (DiMauro et al. (2001) Am. J. Med. Genet. 106, 18-26; Leonard et al. (2000) Lancet. 355, 299-304).
  • the invention also describes use , of these compounds for the treatment of mitochondrial disorders, including but not limited to Friedreich's ataxia, Leber's Hereditary Optic Neuropathy, Keams-Sayre Syndrome, Mitochondrial Encephalomyopathy with Lactic Acidosis and Stroke-Like Episodes and more generally, any disease associated with impairment of energy production and mitochondrial function.
  • mitochondrial disorders including but not limited to Friedreich's ataxia, Leber's Hereditary Optic Neuropathy, Keams-Sayre Syndrome, Mitochondrial Encephalomyopathy with Lactic Acidosis and Stroke-Like Episodes and more generally, any disease associated with impairment of energy production and mitochondrial function.
  • the disclosure rovides compounds of formula (I):
  • R 1 is C 4 -C 2 o alkyl, C 4 -C 20 alkenyl, or C 4 -C 20 alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, -Ce alkyl, halo(C 1 -C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , -CON(R 5 ) 2 , cycloalkyl, aryl, heteroaryl, and heterocycle, wherein each cycloalkyl, aryl, heteroaryl, and heterocycle are optionally substituted with R 9 ;
  • each R 5 independently is hydrogen, Ci-Ce alkyl, halo ⁇ -Ce alkyl), cycloalkyl, aryl, heteroaryl, or heterocycle;
  • each R 9 independently is halogen, cyano, nitro, -N 3 , C ⁇ -C alkyl, halo(C 1 -C 6 alkyl), Ci-C 6 alkoxy, amino, C]-C 6 alkylamino, or diCi-C 6 alkylamino;
  • R 2 is C1-C4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, or Q-Q alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, C C 6 alkyl, halo(Ci-C 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or Ci-C 6 all yl;
  • R 3 is hydrogen, halogen, Ci-C 6 alkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, or -OR 7 , where R 7 is hydrogen, ⁇ -C alkyl, halo(C!-C6 alkyl), or -CO(CrC6 alkyl); and
  • R 4 is hydrogen, C!-C 6 alkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, C 3 -C 8 cycloallcyl(Ci-C 6 alkyl), aryl(Ci[ ⁇ C 6 alkyl), heteiOaryl(Cj[-C 6 alkyl), or heterocycleid-Ce alkyl), wherein each cycloalkyl, aryl, heteroaryl, and heterocycle are optionally substituted with R 7 , wherein each R 8 independently is halogen, cyano, nitro, -N 3 , Ci-C 6 alkyl, halo(C 1 -C 6 alkyl), Ci-Ce alkoxy, amino, CrCealkylamino, or did-Cealkylamino.
  • compositions comprising the compounds and salts of the disclosure and an appropriate earlier, excipient or diluent.
  • the exact nature of the carrier, excipient or diluent will depend upon the desired use for the composition, and may range from being suitable for veterinary uses to being suitable for human use.
  • the compositions may optionally include one or more additional compounds suitable for a use.
  • Another aspect of the disclosure provides methods of treating or suppressing diseases associated with decreased mitochondrial function resulting in diminished ATP production and/or oxidative stress and/or lipid peroxidation, comprising administering an effective amount of the compound and salts of the disclosure.
  • Another aspect of the disclosure provides a method of treating or suppressing one or more of Friedreich's ataxia, Leber's Hereditary Optic Neuropathy, Kearns-Sayre Syndrome, Mitochondrial Encephalomyopathy with Lactic Acidosis and Stroke-Like Episodes, or Leigh syndrome, comprising administering an effective amount of the compound and salts of the disclosure.
  • Another aspect of the disclosure provides a method of treating or suppressing one or more of obesity, atherosclerosis, Parkinson's Disease, cancer, heart failure, myocardial infarction (MI), Alzheimer's Disease , Huntington's Disease, schizoplirenia, bipolar- disorder, fragile X syndrome, chronic fatigue syndrome, and Leigh syndrome, comprising administering an effective amount of the compound and salts of the disclosure.
  • MI myocardial infarction
  • Alzheimer's Disease Huntington's Disease
  • schizoplirenia bipolar- disorder
  • fragile X syndrome fragile X syndrome
  • chronic fatigue syndrome chronic fatigue syndrome
  • Leigh syndrome a method of treating or suppressing one or more of obesity, atherosclerosis, Parkinson's Disease, cancer, heart failure, myocardial infarction (MI), Alzheimer's Disease , Huntington's Disease, schizoplirenia, bipolar- disorder, fragile X syndrome, chronic fatigue syndrome, and Leigh syndrome
  • the compounds of the invention increase ATP concentration in CoQio deficient cells.
  • the compounds of the invention inhibit lipid peroxidation and prevent reactive oxygen species (ROS) production in cells depleted of the antioxidant glutathione (GSH) using the chemical diethyl maleate.
  • ROS reactive oxygen species
  • GSH antioxidant glutathione
  • these compounds all prevented ROS- dependent cell death after the cells were depleted of GSH.
  • the antioxidant potential of the compounds described above is significantly increased compared to that of a-tocopherol( - TOH) and idebenone; therefore, these compounds have the potential of improved efficacy in clinical applications compared to a-tocopherol and idebenone.
  • FIG. 1 Representative flow cytometric histograms overlay showing ROS production in CEM cells. Following pretreatment with the indicated compounds (1, 2.5 and 5 ⁇ ) for 16 h, the cells were treated with 5 mM diethyl maleate (DEM) for 50 min to deplete glutathione. The cells were washed in phosphate-buffered saline, and suspended in phosphate-buffered saline containing 20 mM glucose. Cells were loaded with 10 ⁇ dichlorodihydrofluoresceindiacetate (DCFH-DA) for 20 min, and the green fluorescence (DCF) was measured by flow cytometry using the FL1-H channel. The figure shows a representative example of three independent experiments.
  • DEM diethyl maleate
  • FIG. 3 Representative flow cytometric two-dimensional color density dot plot analyses of mitochondrial membrane potential ( ⁇ ⁇ 1 ) in leulcemia CEM cells stained with 250 nM TMRM and analyzed using FL2-H channel as described in the experimental section. The percentage of cells with intact ⁇ ⁇ is indicated in the top right quadrant of captions. Representative example from at least three independent experiments. A total of 10000 events were recorded for each sample and analyzed with the CellQuest software (BD Biosciences).
  • Figure 4 A bar graph representation of means the percentage of cells with intact ⁇ ⁇ , recorded by FACS. Data are expressed as means ⁇ SEM of three independent experiments ran in duplicate.
  • the disclosure provides com ounds of formula (I):
  • bond " r ⁇ z" is a single or a double bond
  • R 1 is C 4 -C 20 alkyl, C 4 -C 2 o alkenyl, or C 4 -C 20 alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(C ! -C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , -CON(R s ) 2 , cycloalkyl, aryl, heteroaryl, and heterocycle, wherein each cycloalkyl, aryl, heteroaryl, and heterocycle are optionally substituted with R 9 ;
  • each R 5 independently is hydrogen, Ci-C 6 alkyl, halo(Cj-C 6 alkyl), cycloalkyl, aryl, heteroaryl, or heterocycle;
  • each R 9 independently is halogen, cyano, nitro, -N 3 , Ci-C alkyl, halo(Ci-C6 alkyl), Ci-C 6 alkoxy, amino, CrCealkylamino, or diCi-C 6 alkylamino;
  • R is C C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, or C 1 -C 4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-Ce alkyl, halo(C 1 -C 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or Cr-C 6 alkyl;
  • R 3 is hydrogen, halogen, Ci-C 6 alkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, or -OR 7 , where R 7 is hydrogen, C C 6 alkyl, halo(C!-C6 alkyl), or -00( ⁇ ! -0 6 alkyl); and
  • R 4 is hydrogen, Ci-C 6 alkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, C 3 -C 8 cycloalkyl(Ci-C 6 alkyl), aryl(Ci-C6 alkyl), heteroaiyl(Ci-C 6 alkyl), or heterocycle(C 1 -C 6 alkyl), wherein each cycloalkyl, aryl, heteroaryl, and heterocycle are optionally substituted with R 7 ,
  • each R independently is halogen, cyano, nitro, -N 3 , Cj-C 6 alkyl, halo(C 1 -C 6 alkyl), Cj-C 6 alkoxy, amino, C Qalkylamino, or diC -Ceall ylamino.
  • the disclosure provides compounds of formula (I), which are of formula (II):
  • the disclosure provides compounds of formula (I), which are of foi Tiula (III):
  • the disclosure provides compounds of formulae (I)-(III), wherein R 4 is hydrogen or Ci-Ce alkyl. In another embodiment, R 4 is hydrogen or C C 3 alkyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 4 is hydrogen.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 4 is Ci-C 3 alkyl. In one embodiment, R 4 is methyl or ethyl. In yet another embodiments R 4 is methyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 2 is Ci-C 4 alkyl, optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(Ci-C 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or d- C 6 alkyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R is unsubstituted Q-C 4 alkyl. In one embodiment, R is unsubstituted Ci-C 2 alkyl. In another embodiment, R is methyl or ethyl. In yet another embodiment, R 2 is methyl. In one embodiment, the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R is hydrogen, halogen, d-C 6 alkyl, or -OR , where R 7 is hydrogen, Ci-Ce alkyl, halo(Ci-C 6 allcyl), or -CO(Ci-C 6 alkyl).
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R is hydrogen or halogen.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 3 is hydrogen.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 3 is halogen.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 3 is Ci-C 6 allcyl, or -OR 7 , where R 7 is hydrogen, C ⁇ - C 6 alkyl, haloCd-Ce allcyl), or -CO(Ci-C 6 alkyl).
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 3 is Ci-C 6 allcyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 3 is -OR 7 , where R 7 is hydrogen, d-C 6 alkyl, halo(d-C 6 allcyl), or -CO(Ci-C 6 allcyl).
  • R 3 is -OR 7 , where R 7 is hydrogen, C]-C 6 allcyl, or -CO(C ! -C 6 allcyl).
  • R 3 is -OH.
  • R is -0(CH 3 ).
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 3 is -OCO(C ! -C 6 allcyl). Another embodiment provides compounds where R 3 is -OCOCH 3 .
  • the disclosure provides compounds as described ' above with any reference to formulae (I)-(III), wherein R 1 is C 4 -C 20 alkyl, C 4 -C 20 alkenyl, or C 4 -C 20 allcynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, d-Ce allcyl, halo(d-C 6 allcyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, d-C 6 allcyl, halo(Ci-C 6 allcyl), cycloallcyl, aryl, heteroaryl, or heterocycle.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is C 4 -C 20 allcyl, C 4 -C 20 alkenyl, or C 4 -C 20 allcynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(Ci-C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, C ! -C 6 alkyl, or halo(Ci-C 6 alkyl).
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is C 4 -C 20 alkyl, optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(Ci-C 6
  • each R 5 independently is hydrogen, Ci-C 6 alkyl, halo(Ci-C 6 alkyl), cycloalkyl, aryl, heteroaryl, or heterocycle; and wherein each R 9 independently is halogen, cyano, nitro, -N 3 , C C 6 alkyl, halo(Cj-C 6 alkyl), Ci-C 6 alkoxy, amino, Ci-C 6 alkylamino, or diQ- C 6 alkylamino.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is C 4 -C 20 alkyl, optionally substituted with one to four substituents selected from halogen, cyano, nitro, C C 6 alkyl, halo(Ci-C 6
  • each R 5 independently is hydrogen, Ci-C 6 alkyl, or alkyl); and wherein each R 9 independently is halogen, cyano, nitro, -N 3 , C C 6 alkyl, halo(Ci-C 6 alkyl), Ci-C 6 alkoxy, amino, Ci-Ceallcylamino, or diCi-Cealkylamino.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is C 4 -C 20 alkyl, optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(CrC 6
  • each R 5 independently is hydrogen, Q- C 6 alkyl, or halo(Ci-C 6 alkyl).
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is C 4 -C 20 alkyl, optionally substituted with one to four substituents selected from halogen, Ci-C 6 alkyl, halo(C 1 -C 6
  • R 1 is C 4 -C 20 alkyl optionally substituted with one to four -OH groups.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is C 5 -C 20 alkyl, optionally substituted with one to four substituents selected from halogen, cyano, nitro, C ⁇ -C alkyl, halo(Cj-C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , -CON(R 5 ) 2 , cycloalkyl, aryl, heteroaryl, and heterocycle, wherein each cycloalkyl, aryl, heteroaryl, and heterocycle are optionally substituted with R 9 ; where each R 5 independently is hydrogen, C ⁇ -Ce alkyl, halo(C 1 -C 6 alkyl), cycloalkyl, aryl, heteroaryl, or heterocycle; and wherein each R 9 independently is halogen, cyano, nitro, -N 3 ,
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is C 5 -C 20 alkyl, optionally substituted with one to four substituents selected from halogen, cyano, nitro, C C 6 alkyl, halo(CrC 6
  • each R 5 independently is hydrogen, i-Ce alkyl, or halo(Ci-C 6 alkyl); and wherein each R 9 independently is halogen, cyano, nitro, -N 3 , i-C alkyl, halo(C!-C 6 alkyl), ⁇ -Ce alkoxy, amino, C Ceallcylamino, or diCi-Cealkylamino.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is ⁇ 5 - ⁇ 20 alkyl, optionally substituted with one to four substituents selected from halogen, C C 6 alkyl, halo(d-C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -C0N(R 5 ) 2 ; where each R 5 independently is hydrogen, C ⁇ -C alkyl, or halo(CrC 6 alkyl).
  • R 1 is C5-C20 alkyl optionally substituted with one to four -OH groups.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is C 6 -C 2 o alkyl, optionally substituted with one to four substituents selected from halogen, cyano, nitro, C ⁇ -Ce alkyl, halo(Ci-C6
  • each R 5 independently is hydrogen, C ⁇ -Ce alkyl, halo(Ci-C 6 alkyl), cycloalkyl, aryl, heteroaryl, or heterocycle; and wherein each R 9 independently is halogen, cyano, nitro, -N 3 , Ci-C 6 alkyl, halo(Ci-C 6 alkyl), C ⁇ -C& alkoxy, amino, Ci-C 6 alkylamino, or diCi- C 6 alkylamino.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is C 6 -C 20 alkyl, optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(Ci-C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , -CON(R 5 ) 2 , cycloalkyl, aryl, heteroaiyl, and heterocycle, wherein each cycloalkyl, aryl, heteroaiyl, and heterocycle are optionally substituted with R 9 ; where each R 5 independently is hydrogen, C ⁇ -C alkyl, or halo ⁇ -Ce alkyl); and wherein each R 9 independently is halogen, cyano, nitro, -N 3 , Ci-Ce alkyl, halo(Ci-C6 alkyl), C]-C 6 alkoxy, amino
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is C 6 -C 20 alkyl, optionally substituted with one to four substituents selected from halogen, Ci-C 6 alkyl, halo(Ci-C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, Ci-C 6 alkyl, or halo(C 1 -C 6 alkyl).
  • R 1 is C 6 -C 20 alkyl optionally substituted with one to four -OH groups.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is C 7 -C 2 o alkyl, optionally substituted with one to four substituents selected from halogen, cyano, nitro, C C 6 alkyl, halo(Ci-C 6
  • each R 5 independently is hydrogen, C C 6 alkyl, halo(C 1 -C 6 alkyl), cycloalkyl, aryl, heteroaiyl, or heterocycle; and wherein each R 9 independently is halogen, cyano, nitro, -N 3 , C -Ce alkyl, halo ⁇ -Ce alkyl), Ci-C 6 alkoxy, amino, CrCealkylamino, or diC
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is C 7 -C 20 alkyl, optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(Ci-C 6
  • each R 5 independently is hydrogen, C C 6 alkyl, or halo(Ci-C 6 alkyl); and wherein each R 9 independently is halogen, cyano, nitro, -N 3 , Q-C6 alkyl, halo(Ci-C 6 alkyl), Ci-C 6 alkoxy, amino, Ci-Cealkylamino, or diCi-C 6 alkylamino.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is C 7 -C 20 alkyl, optionally substituted with one to four substituents selected from halogen, C C 6 alkyl, halo(Ci-C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, Q- 5 alkyl, or halo(Ci-C 6 alkyl).
  • R 1 is C 7 -C2 0 alkyl optionally substituted with one to four -OH groups.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is unsubstituted C 4 -C 20 alkyl. In yet another embodiment, the disclosure provides compounds as described above with any reference to fomiulae (I)-(III), wherein R 1 is unsubstituted C 5 -C 20 alkyl. In yet another embodiment, the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is unsubstituted C 6 -C 20 alkyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is unsubstituted C 7 -C 2 o alkyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is straight-chain unsubstituted alkyl.
  • R 1 is straight-chain unsubstituted alkyl.
  • the straight-chain unsubstituted alkyl having 4-20 cai'bon atoms, or 5-20 carbon atoms, or 6-20 carbon atoms, or 7-20 carbon atoms.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R 1 is pentyl, decyl or hexadecyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C 4 -C 2 o alkyl, C 4 -C2 0 alkenyl, or C4-C20 alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, C]-C 6 alkyl, halo(Cj-C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , -CON(R 5 ) 2 , cycloalkyl, aryl, heteroaryl, and heterocycle, wherein each cycloalkyl, aryl, heteroaryl, and heterocycle are optionally substituted with R 9 ;
  • each R 5 independently is hydrogen, Ci-C 6 alkyl, or halo(Cj-C 6 alkyl);
  • each R 9 independently is halogen, cyano, nitro, -N 3 , i-Ce alkyl, halo(C 1 -C 6 alkyl), Ci-C 6 alkoxy, amino, CrCealkylamino, or did-Cealkylamino;
  • R is C]-C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, or C ! -C 4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(Ci-C 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or Ci-C 6 alkyl; R 3 is Ci-C 6 alkyl or -OR 7 , where R 7 is hydrogen, Ci-C 6 alkyl, halo(Ci-C 6 allcyl), or -CO(C r
  • R 4 is hydrogen, or Ci-C 6 alkyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C 4 -C 20 alkyl, C 4 -C 20 alkenyl, or C4-C 20 alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-Ce alkyl, halo(C 1 -C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, Ci-C 6 alkyl, or halo(C 1 -C 6 alkyl);
  • R 2 is C C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, or CpC 4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 allcyl, halo(Ci-C 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or C C 6 allcyl;
  • R 3 is Ci-Ce allcyl or -OR 7 , where R 7 is hydrogen, d-C 6 allcyl, halo(Ci-C 6 alkyl), or -CO(Ci- C 6 allcyl); and
  • R 4 is hydrogen, or C ⁇ -Ce allcyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C4-C 20 allcyl, C -C 20 alkenyl, or C 4 -C 2 o allcynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Q-Cg allcyl, halo(Ci-C 6 allcyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, Cj-C 6 allcyl, or halo(C ! -C 6 alkyl);
  • R is Q-C4 allcyl, C 2 -C 4 alkenyl, C 2 -C4 allcynyl, or CrQ alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 allcyl, haloCQ-Ce allcyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or C C 6 allcyl;
  • R 3 is -OR 7 , where R 7 is hydrogen, C C 6 allcyl, halo(Ci-C 6 allcyl), or -COCCrCe allcyl); and
  • R 4 is hydrogen, or C C 6 allcyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C 4 -C 20 allcyl, C4-C 20 alkenyl, or C 4 -C 2 o allcynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci- e allcyl, haloiC Ce alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, Ci-C 6 alkyl, or halo(C]i-C 6 alkyl);
  • R is Q-Q alkyl, C 2 -C 4 alkenyl, or C 2 -C 4 all ynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, C]-C 6 allcyl, halo(C!-C 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 5 independently is hydrogen or C C 6 alkyl;
  • R 3 is -OR 7 , where R 7 is hydrogen, Ci-C 6 alkyl, halo(C C 6 alkyl), or -CO CrCe alkyl); and R 4 is d-C 6 allcyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C 5 -C 20 alkyl, C 5 -C 20 alkenyl, or C 5 -C 2 o alkynyl, each optionally substituted with one to foui- substituents selected from halogen, cyano, nitro, C C 6 allcyl, halo(Ci-C 6 allcyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , -CON(R 5 ) 2 , cycloallcyl, aryl, heteroaryl, and heterocycle, wherein each cycloallcyl, aryl, heteroaryl, and heterocycle are optionally substituted with R 9 ;
  • each R 5 independently is hydrogen, Q-Cg allcyl, or halo(Ci-C 6 allcyl);
  • each R 9 independently is halogen, cyano, nitro, ⁇ N 3 , Ci-C 6 allcyl, halo(C 1 -C 6 allcyl), Ci-C 6 alkoxy, amino, C Qallcylamino, or diC Qallcylamino;
  • R" is Ci-C 4 allcyl, C 2 -C alkenyl, C 2 -C 4 allcynyl, or C 1 -C 4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 allcyl, halo(Cj-C 6 allcyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or C!-C 6 allcyl;
  • R 3 is C C 6 allcyl or -OR 7 , where R 7 is hydrogen, Ci-C 6 allcyl, halo(Ci-C 6 allcyl), or -CO(Ci-
  • R 4 is hydrogen, or Ci-C 6 allcyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C 5 -C 2 o allcyl, C 5 -C 20 alkenyl, or C5-C20 alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 allcyl, halo(C]!-C6 allcyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, Ci-C 6 allcyl, or halo(C 1 -C 6 allcyl);
  • R is Q-C4 allcyl, C 2 -C 4 alkenyl, C 2 -C 4 allcynyl, or C Q alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, .nitro, Cj-C 6 allcyl, halo(Ci-C 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or C ⁇ -Ce allcyl;
  • R 3 is C C 6 alkyl or -OR 7 , where R 7 is hydrogen, d-C 6 alkyl, halo(C r C 6 alkyl), or -CO(Cr
  • R 4 is hydrogen, or i-Ce alkyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C 5 -C 2 o alkyl, C 5 -C 2 o alkenyl, or C5-C 20 alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(Ci-C 6 allcyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, Ci-C 6 allcyl, or halo(Ci-C 6 allcyl);
  • R is Ci-C 4 allcyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, or Ci-C 4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 allcyl, halo(Ci-C 6 allcyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or Ci-C6 allcyl;
  • R 3 is -OR 7 , where R 7 is hydrogen, d-C 6 allcyl, .halo(Ci-C 6 alkyl), or -CO(C r C 6 alkyl); " and
  • R 4 is hydrogen, or Ci-C 6 alkyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C 5 -C 2 o allcyl, C 5 -C 2 o alkenyl, or C 5 -C 20 alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Q-C6 allcyl, haloCCi-Ce allcyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, C ⁇ -Ce allcyl, or halo(C ! -C 6 alkyl);
  • R is Q-C4 allcyl, C 2 -C 4 alkenyl, or C 2 -C 4 alkynyl, each optionally substituted with one to four substituents selected.from halogen, cyano, nitro, C ! -C 6 allcyl, halo(C ! -C 6 allcyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or Ci-Ce allcyl;
  • R 3 is -OR 7 , where R 7 is hydrogen, Ci-C 6 allcyl, halo(Ci-C 6 allcyl), or -CO(Ci-C 6 allcyl); and R 4 is Ci-C 6 allcyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein R is C 6 -C 20 alkyl, C 6 -C 20 alkenyl, or C 6 -C 20 alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(C 1 -C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , -CON(R 5 ) 2 , cycloalkyl, aryl, heteroaryl, and heterocycle, wherein each cycloalkyl, aryl, heteroaryl, and heterocycle are optionally substituted . with R 9 ;
  • each R 5 independently is hydrogen, C C 6 alkyl, or halo(CrC 6 all y 1);
  • each R 9 independently is halogen, cyano, nitro, -N 3 , Q-Qs alkyl, halo(Ci-C 6 alkyl), CpC 6 alkoxy, amino, CrCealkylamino, or diCrCeallcylamino;
  • R 2 is CrC 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, or Q-C4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, C ⁇ -Ce alkyl, halo d-Ce alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or Ci-C 6 all yl;
  • R 3 is Ci-C 6 alkyl or -OR 7 , where R 7 is hydrogen, C . -Ce alkyl, halo(Ci-C 6 alkyl), or -CO(Ci-
  • R 4 is hydrogen, or C]-C 6 alkyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C6-C 20 alkyl, C 6 -C 20 alkenyl, or C 6 -C 20 alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, CI-CQ allcyl, halo(d-C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, Cj-C 6 alkyl, or hak Q-Ce alkyl);
  • R is C 1 -C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, or C1-C4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-Ce allcyl, halo(Ci-C 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or Ci-C 6 allcyl;
  • R 3 is Ci-Ce alkyl or -OR 7 , where R 7 is hydrogen, C r C 6 alkyl, halo(Ci-C 6 allcyl), or -CO(C C 6 allcyl); and
  • R 4 is hydrogen, or C C 6 allcyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C 6 -C 20 allcyl, C 6 -C 20 alkenyl, or C 6 -C 20 alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 allcyl, halo(C 1 -C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, C C 6 alkyl, or halo(Ci-C 6 alkyl);
  • R 2 is Ci-C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 allcynyl, or C1-C4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(Ci-C 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or Ci-C 6 alkyl;
  • R 3 is -OR 7 , where R 7 is hydrogen, Ci-C 6 alkyl, halo(C r C 6 alkyl), or -CO(C C 6 alkyl); and R 4 is hydrogen, or Ci-C 6 alkyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C 6 -C 20 alkyl, C 6 -C 20 alkenyl, or C 6 -C 2 o alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, C ⁇ -C alkyl, halo(C 1 -C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, Ci-C 6 alkyl, or halo(CrC 6 alkyl);
  • R 2 is C1-C4 alkyl, C 2 -C 4 alkenyl, or C 2 -C 4 alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(Cj-C 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or C C 6 alkyl;
  • R 3 is -OR 7 , where R 7 is hydrogen, C r C 6 alkyl, halo(Ci-C 6 alkyl), or -CO(C C 6 alkyl); and R 4 is Ci-C 6 alkyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C 7 -C 20 alkyl, C 7 -C 20 alkenyl, or C 7 -C 2 o alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(Cj-C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , -CON(R 5 ) 2 , cycloalkyl, aryl, heteroaryl, and heterocycle, wherein each cycloalkyl, aryl, heteroaryl, and heterocycle are optionally substituted with R 9 ;
  • each R 5 independently is hydrogen, Cj-C6 alkyl, or halo(Ci-C 6 alkyl);
  • each R 9 independently is halogen, cyano, nitro, -N 3 , C ! -C 6 alkyl, halo ⁇ -Ce alkyl), Ci-Ce alkoxy, amino, Q-Cealkylamino, or diCi-C 6 alkylamino;
  • R is Cr-C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, or Ci-C 4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, C C 6 alkyl, halo(CrC 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or C]-C 6 alkyl;
  • R 3 is C 1 -Q5 alkyl or -OR 7 , where R 7 is hydrogen, Ci-C 6 alkyl, halo(Ci-C 6 alkyl), or -CO(d-
  • R 4 is hydrogen, or Ci-C 6 alkyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C 7 -C 20 alkyl, C 7 -C 20 alkenyl, or C 7 -C 2 o alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(Ci-C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , -CON(R 5 ) 2 , cycloalkyl, aryl, heteroaryl, and heterocycle, wherein each cycloalkyl, aryl, heteroaryl, and heterocycle are optionally substituted with R 9 ;
  • each R 5 independently is hydrogen, Ci-C 6 alkyl, or halo(C 1 -C 6 alkyl);
  • each R 9 independently is halogen, cyano, nitro, -N 3 , C ⁇ -Ce alkyl, halo(Ci-C 6 alkyl), Ci-C 6 alkoxy, amino, Q-Cealkylammo, or diQ-Cealkylamino;
  • R is C 1 -C4 alkyl, C 2 -C4 alkenyl, C 2 -C 4 alkynyl, or C 1 -C4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(Ci-C 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or Cj-C 6 alkyl;
  • R 3 is-OR 7 , where R 7 is hydrogen, C r C 6 alkyl, halo(Ci-C 6 alkyl), or -CO(C r C 6 alkyl); and R 4 is hydrogen, or Cj[-C 6 all yl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C 7 -C 2 o alkyl, C 7 -C 20 alkenyl, or C 7 -C 20 alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(Ci-C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, C C 6 alkyl, or halo ⁇ -d allcyl);
  • R 2 is ⁇ -C4 alkyl, C 2 -C4 alkenyl, C 2 -C 4 allcynyl, or C 1 -C4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 allcyl, haloCd-Ce alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or C C 6 allcyl;
  • R 3 is -OR 7 , where R 7 is hydrogen, C r C 6 allcyl, halo(Ci-C 6 alkyl), or -CO(C 1 -C 6 alkyl); and
  • R 4 is hydrogen, or Ci-C 6 alkyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C7-C 20 ' alkyl, C7-C 20 alkenyl, or C 7 -C 2 o alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, C C 6 alkyl, halofOrCe alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , -CON(R 5 ) 2 , cycloalkyl, aryl, heteroaryl, and heterocycle, wherein each cycloalkyl, aryl, heteroaryl, and heterocycle are optionally substituted with R 9 ;
  • each R 5 independently is hydrogen, Ci-C 6 alkyl, or halo(C 1 -C 6 alkyl);
  • each R 9 independently is halogen, cyano, nitro, -N 3 , Ci-C 6 alkyl, halo(Ci-C 6 alkyl), C ! -C 6 alkoxy, amino, Q-Cealkylamino, or diC Cealkylamino;
  • R 2 is C C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, or Ci-C 4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 allcyl, halo(Ci-C 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 5 independently is hydrogen or C ⁇ -Ce allcyl;
  • R 3 is-OR 7 , where R 7 is hydrogen, alkyl); and R 4 is Ci-C 6 alkyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C 7 -C 20 alkyl, C 7 -C 20 alkenyl, or C 7 -C 20 alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, C ! -C 6 allcyl, halo(Ci-C 6 allcyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, alkyl);
  • R 2 is Ci-C 4 alkyl, C 2 -C 4 alkenyl, or C 2 -C 4 alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(Ci-C 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or i-C allcyl;
  • R 3 is -OR 7 , where R 7 is hydrogen, Ci-C 6 allcyl, halo(C C 6 allcyl), or -COCC Ce allcyl); and R 4 is d-C 6 alkyl.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C 7 -C 2 o allcyl, C 7 -C 2 o alkenyl, or C 7 -C 20 allcynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, haloCQ-Q allcyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , -CON(R 5 ) 2 , cycloalkyl, aryl, heteroaryl, and heterocycle, wherein each cycloalkyl, aryl, heteroaryl, and heterocycle are optionally substituted with R 9 ;
  • each R 5 independently is hydrogen, Q-Q alkyl, or halo ⁇ -d alkyl
  • each R 9 independently is halogen, cyano, nitro, -N 3 , CrC 6 alkyl, halo(Ci-C 6 alkyl), Cj-C 6 alkoxy, amino, Ci-dall ylamino, or diQ-Cealkylamino;
  • R is C1-C4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, or C1-C4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-Cs alkyl, haloCd-Ce alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or Ci-Ce alkyl;
  • R 3 is hydrogen, halogen, Q-C6 alkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, or -OR 7 , where R 7 is hydrogen, Ci-C 6 alkyl, halo(Ci-C 6 alkyl), or -00(0 ⁇ 0, alkyl); and
  • R 4 is hydrogen, Ci-C 6 alkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, C3-Cgcycloalkyl(Ci-C6 alkyl), aryl(Ci-C 6 alkyl), heteroaryl(Ci-C6 alkyl), or heterocycle(Ci-G6 alkyl), wherein each cycloalkyl, aryl, heteroaryl, and heterocycle are optionally substituted with R 7 , wherein each R 8 independently is halogen, cyano, nitro, -N 3 , Ci-C 6 alkyl, halot rCe alkyl), C1-C6 alkoxy, amino, CrCealkylamino, or diCi-Cealkylamino.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C -C 20 alkyl, C 7 -C 20 alkenyl, or C 7 -C 20 alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, C ⁇ -C alkyl, halo(C ! -C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, C Q alkyl, or halo(C ! -C 6 alkyl);
  • R 2 is C1-C4 alkyl, C 2 -C 4 alkenyl, C 2 -C4 alkynyl, or C 1 -C 4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, C ⁇ -C alkyl, halo(Ci-C 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or Ci-C 6 alkyl;
  • R 3 is hydrogen, halogen, C1-C6 alkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, or -OR 7 , where R 7 is hydrogen, Ci-C 6 alkyl, haloCd-Ce alkyl), or -COCd-Ce alkyl); and
  • R 4 is hydrogen, Ci-C 6 alkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, C3-C 8 cycloalkyl(Ci-C 6 alkyl), aryl(Cj-C 6 alkyl), heteroaryl(Ci-C 6 alkyl), or heterocycle(Ci-C 6 alkyl), wherein each cycloalkyl, aryl, heteroaryl, and heterocycle are optionally substituted with R 7 , wherein each R 8 independently is halogen, cyano, nitro, -N 3 , d-C 6 alkyl, halo(d-C 6 alkyl), d-C 6 alkoxy, amino, Ci-C 6 alkylamino, or did-C 6 alkylamino.
  • the disclosure provides compounds as described above with any reference to formulae (I)-(III), wherein
  • R 1 is C 7 -C 2 o alkyl, C 7 -C 2 o alkenyl, or C 7 -C 2 o alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, CI-CQ alkyl, halo(Ci-C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, Ci-C 6 alkyl, or haloiQ-Q alkyl);
  • R is C C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 allcynyl, or Ci-C 4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(C C 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or Ci-Ce alkyl;
  • R 3 is Ci-C 6 alkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, or -OR 7 , where R 7 is hydrogen, Ci-C 6 alkyl, halo(C r C 6 alkyl), or -CO(Ci-C 6 alkyl); and
  • R 4 is hydrogen, C ⁇ -Ce alkyl, C 2 -C 6 alkenyl, C 2 -C 6 allcynyl, C CscycloalkyliC Ce alkyl), aryl(CrC 6 alkyl), heteroarylCC Ce alkyl), or heterocycleiC Ce alkyl), wherein each cycloallcyl, aryl, heteroaiyl, and heterocycle are optionally substituted with R 7 , wherein each R independently is halogen, cyano, nitro, -N 3 , Ci-C alkyl, halo(Ci-C6 alkyl), CI-CQ alkoxy, amino, Ci-Cealkylaimno, or diC Ceallcylamino.
  • R 1 is C 7 -C 2 o alkyl, C 7 -C 20 alkenyl, or C 7 -C 20 alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, C]-C 6 alkyl, halo(Ci-C 6
  • each R 5 independently is hydrogen, Ci-C 6 alkyl, or halo(Cj[-C6 alkyl);
  • each R 9 independently is halogen, cyano, nitro, -N 3 , Ci-C 6 alkyl, halo ⁇ -Ce alkyl), C][-C 6 alkoxy, amino, Q-Ceallcylamino, or diCi-Cealkylamino;
  • R 2 is C1-C4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 allcynyl, or C C 4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(Ci-C 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or C ⁇ -C(, alkyl;.
  • R 3 is d-Ce alkyl or -OR 7 , where R 7 is hydrogen, C r C 6 alkyl, halo(Ci-C 6 alkyl), or -CO(C
  • R 4 is hydrogen, CrC 6 alkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, Cs-CgcycloallcylCQ-Q allcyl), ary ⁇ C Ce alkyl), heteroaryl(Ci-C 6 alkyl), or lieterocyclei rCe allcyl), wherein each cycloallcyl, aryl, heteroaryl, and heterocycle are optionally substituted with R 7 , wherein each R 8 independently is halogen, cyano, nitro, -N 3 , Ci-C 6 alkyl, halo(C 1 -C 6 alkyl), Ci-C 6 alkoxy, amino, Ci-C 6 allcylamino, or diCi-Cealkylamino.
  • R 1 is C 7 -C 20 allcyl, C 7 -C 20 alkenyl, or C 7 -C 20 allcynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 allcyl, lialo(Ci-C 6 allcyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, CrQ alkyl, or halo(Ci-C 6 allcyl);
  • R is C 1 -C4 allcyl, C 2 -C 4 alkenyl, C 2 -C 4 allcynyl, or C 1 -C4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, C C 6 allcyl, hak CrCe allcyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or Ci-C 6 allcyl;
  • R 3 is Ci-C 6 allcyl or -OR 7 , where R 7 is hydrogen, Ci-C 6 allcyl, halo(Ci-C 6 allcyl), or -CO(Ci- C 6 allcyl); and
  • R 4 is hydrogen, Ci-C 6 allcyl, C 2 -C 6 alkenyl, C 2 -C 6 allcynyl, Cs-Cscycloalky ⁇ C Ce allcyl), aryl(C!-C6 allcyl), heteroaryl(Ci-C6 allcyl), or heterocycleid-Ce alkyl), wherein each cycloallcyl, aryl, heteroaryl, and heterocycle are optionally substituted with R 7 , wherein each R 8 independently is halogen, cyano, nitro, -N 3 , Ci-C 6 allcyl, halo(Ci-C 6 allcyl), C ⁇ -C alkoxy, amino, C Ceallcylamino, or diCi-C 6 allcylamino.
  • R 1 is C 7 -C 20 allcyl, C 7 -C 20 alkenyl, or C 7 -C 20 allcynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, Ci-C 6 alkyl, halo(C] -C6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , -CON(R 5 ) 2 , cycloallcyl, aryl, heteroaryl, and heterocycle, wherein each cycloallcyl, aryl, heteroaryl, and heterocycle are optionally substituted with R 9 ;
  • each R 5 independently is hydrogen, C ! -C 6 allcyl, or halo(Ci-C 6 allcyl);
  • each R 9 independently is halogen, cyano, nitro, -N 3 , C 1 -C6 allcyl, halo(C!-C6 alkyl), Ci-C 6 alkoxy, amino, C Ceallcylamino, or diQ-Qallcylamino;
  • R 2 is -C4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, or C C 4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, ⁇ -C alkyl, halo(Ci-C 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or Q- alkyl;
  • R 3 is-OR 7 , where R 7 is hydrogen, C C 6 alkyl, halo(Ci-C 6 alkyl), or -CO(C r C 6 alkyl); and R 4 is hydrogen, C C 6 alkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, Cs-Qcycloalky ⁇ C Ce alkyl), aryl(Ci-C6 alkyl), heteroaiyl(Ci-C 6 alkyl), or heterocycle(Ci-C 6 alkyl), wherein each cycloalkyl, aryl, heteroaryl, and heterocycle are optionally substituted with R , wherein each R 8 independently is halogen, cyano, nitro, -N 3 , C ⁇ -C alkyl, halo ⁇ -Ce alkyl), Q-Ce alkoxy, amino, C Cealkylamino, or diCi-Cealkylamino.
  • the disclosure provides compounds as described above . with any reference to formulae (I)-(III), wherein
  • R 1 is C 7 -C 20 alkyl, C 7 -C 20 alkenyl, or C 7 -C 2 o alkynyl, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, C C 6 alkyl, halo(Ci-C 6 alkyl), -OR 5 , -NR 5 2 , -C0 2 R 5 , and -CON(R 5 ) 2 ; where each R 5 independently is hydrogen, CI-CQ alkyl, or halo(Ci[-C 6 alkyl);
  • R 2 is C C 4 alkyl, C 2 -C 4 alkenyl, C 2 -C 4 alkynyl, or C ! -C 4 alkoxy, each optionally substituted with one to four substituents selected from halogen, cyano, nitro, C]i-C 6 alkyl, halo(Ci-C 6 alkyl), -OR 6 , -NR 6 2 , -C0 2 R 6 , and -CONR 6 2 ; where each R 6 independently is hydrogen or Q-Q alkyl;
  • R 3 is-OR 7 , where R 7 is hydrogen, Ci-C 6 alkyl, haloCCrCe alkyl), or -CO(C 1 -C 6 alkyl); and
  • R 4 is hydrogen, Ci-C 6 alkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, alkyl), arylCC Ce alkyl), heteroaryli Ce alkyl), or heterocycle(Ci-C 6 alkyl), wherein each cycloalkyl, aryl, heteroaryl, and heterocycle are optionally substituted with R 7 , wherein each R 8 independently is halogen, cyano, nitro, -N3, Cj-C 6 alkyl, halo(Ci-C 6 alkyl), Ci-C 6 alkoxy, amino, CrCealkylamino, or diCrQalkylamino.
  • the disclosure provides pharmaceutically acceptable salts of compounds of the disclosure.
  • pharmaceutically acceptable salts are those salts that retain substantially one or more of the desired pharmacological activities of the parent compound and which are suitable for administration to humans.
  • Pharmaceutically acceptable salts include acid addition salts formed with inorganic acids or organic acids.
  • Inorganic acids suitable for forming pharmaceutically acceptable acid addition salts include, by way of example and not limitation, hydrohalide acids (e.g., hydrochloric acid, hydrobromic acid, hydriodic, etc.), sulfuric acid, nitric acid, phosphoric acid, and the like.
  • Organic acids suitable for foraiing pharmaceutically acceptable acid addition salts include, by way of example and not limitation, acetic acid, monofluoroacetic acid, difluoroacetic acid, trifluoroacetic acid, propionic acid, hexaiioic acid, cyciopentanepropionic acid, glycolic acid, oxalic acid, pyruvic acid, lactic acid, malonic acid, succinic acid, malic acid, maleic acid, fumaric acid, tartaric acid, citric acid, palmitic acid, benzoic acid, 3-(4-hydroxybenzoyl) benzoic acid, cinnamic acid, mandelic acid, alkylsulfonic acids (e.g., methanesulfonic acid, ethanesulfonic acid, 1,2- ethane-disulfonic acid, 2-hydroxyethanesulfonic acid, etc.), arylsulfonic acids (e.g., benzenesul
  • Pharmaceutically acceptable salts also include salts formed when an acidic proton present in the parent compound is either replaced by an inorganic ion (e.g., an alkali metal ion such as Na + , K + or Li + , an alkaline earth ion such as Ca 2+ or Mg 2+ , an aluminum ion, or an ammonium ion) or coordinates with an organic base (e.g., ethanolamine, diethanolamine, triethanolamine, N-methylglucamine, morpholine, piperidine, dimethylamine, diethylamine, etc.).
  • an inorganic ion e.g., an alkali metal ion such as Na + , K + or Li + , an alkaline earth ion such as Ca 2+ or Mg 2+ , an aluminum ion, or an ammonium ion
  • organic base e.g., ethanolamine, diethanolamine, triethanolamine, N-methylglucamine, morpholine, piper
  • the compounds described herein, as well as the salts thereof, may also be in the ' form of hydrates, solvates and TV-oxides, as are well-known in the ait.
  • Compounds of the disclosure are useful, for example, for treating or suppressing diseases associated with decreased mitochondrial function resulting in diminished ATP production and/or oxidative stress and/or lipid peroxidation in a subject in need of treatment.
  • the present disclosure provides methods of treating conditions including but not limited to Friedreich's ataxia, Leber's Hereditary Optic Neuropathy, Kearns-Sayre Syndrome, Mitochondrial Encephalomyopathy with Lactic Acidosis and Stroke-Like Episodes, and Leigh syndrome in a subject by administering an effective amount of a compound as described above with respect to any of formulae (I)-(III), including a salt or solvate or stereoisomer thereof.
  • the disclosure also provides methods of treating conditions including but not limited to obesity, atherosclerosis, Parkinson's Disease, cancer, heart failure, myocardial infarction (MI), Alzheimer's Disease , Huntington's Disease, schizophrenia, bipolar disorder, fragile X syndrome, chronic fatigue syndrome, and Leigh syndrome, in a subject by administering an effective amount of a compound as described above with respect to any of formulae (I)-(III), including a salt or solvate or stereoisomer thereof.
  • MI myocardial infarction
  • Alzheimer's Disease Huntington's Disease
  • schizophrenia bipolar disorder
  • fragile X syndrome fragile X syndrome
  • chronic fatigue syndrome chronic fatigue syndrome
  • Leigh syndrome Leigh syndrome
  • Friedreich's ataxia is a severe neurodegenerative and cardiodegenerative condition. It is characterized by progressive ataxia of the limbs, muscle weakness, dysarthria, skeletal deformities and cardiomyopathy. While the biochemical basis of the disease is still under investigation, it is strongly associated with insufficient frataxin (Wilson et al. (1997) Nat. Genet.16, 352-357; Wilson et al. (2003) J Neurol. Sci.207, 103-105). In the majority of patients the insufficiency of frataxin is a consequence of an intronic GAA triplet repeat expansion in the gene for frataxin, which results in a significant decrease in its mRNA levels, and ultimately in protein levels as well (Campuzanoet al.
  • Frataxin acts as an iron chaperone during heme biosynthesis (Benczeet al. (2007) J. C.S. Chem. Commun. 1798-1800) and has been shown to be capable of stimulating the in vitro assembly of heme and Fe-S clusters (Park et al. (2003) J Biol. Chem.278, 31340-31351 ; Yoon et al. (2003)J Am Chem. Soc.125, 6078-6084; Yoon et al. (2004) J Biol Chem. 279, 25943-25946).
  • Frataxin can interact physically with mitochondrial electron transport chain proteins, as well as with mitochondrial aconitase (which contains an Fe-S cluster) (Bulteauet al. (2004) Science305, 242-245; Gonzalez-Caboet al. (2005) Hum. Mol Genet.14, 2091-2098). Therefore, frataxin deficiency results in disruption of cellular iron homeostasis, with a progressive iron accumulation in the mitochondrion, and a deficiency in heme and Fe-S clusters.
  • Leber hereditary optic neuropathy is associated with degeneration of retinal ganglion cells and causes progressive loss of vision resulting in various degrees of blindness. Leber hereditary optic neuropathy primarily affects men over the age of 20 and is maternally transmitted due to mutations in the mitochondrial (not nuclear) genome.
  • Keams-Sayre syndrome is a rare neuromuscular disorder typically with onset usually before the age of 20. It is characterized by progressive external ophthalmoplegia (paralysis of the eye muscles) and mild skeletal muscle weakness, hearing loss, loss of coordination, heart problems, and cognitive delays.
  • There are many other names for the Keams-Sayre syndrome including: Chronic progressive external ophthalmoplegia CPEO with myopathy; CPEO with ragged-red fibers; KSS; Mitochondrial cytopathy, Keams-Sayre type; Oculocraniosomatic syndrome; Ophthalmoplegia-plus syndrome; Ophthalmoplegia with myopathy; and Ophthalmoplegia with ragged-red fibers.
  • Mitochondrial Encephalomyopathy with Lactic Acidosis and Stroke-Like Episodes is a progressive mitochondrial disease that involves multiple, organ systems including the central nervous system, cardiac muscle, skeletal muscle, and gastrointestinal system. Symptoms include muscle weakness, stroke-like events, eye muscle paralysis, and cognitive impairment. Leigh syndrome is a degenerative brain disorder is usually diagnosed at a young age (e.g. before age two). Deterioration is often rapid with symptoms such as seizures, dementia, feeding and speech difficulties, respiratory dysfunction, heart problems, and muscle weakness. Prognosis is poor with death typically occurring within a few years of diagnosis.
  • NADH complex I
  • FADH 2 complex II
  • the electron carriers in the respiratory chain include flavins, protein-bound iron-sulfur centers, quinones, cytochromes and copper. There are two molecules that transfer electrons between complexes: coenzyme Q (complex I ⁇ III, and complex II ⁇ III) and cytochrome c (complex III ⁇ IV).
  • coenzyme Q complex I ⁇ III, and complex II ⁇ III
  • cytochrome c complex III ⁇ IV
  • the final electron acceptor in the respiratory chain is 0 2 , which is converted to H 2 0 in complex IV.
  • this mitochondrial coenzyme is present as coenzyme Q 10 , which has a 50-carbon C-substituent that renders the molecule virtually insoluble in water (calculated octanol-water partition coefficient >10 20 ) (James et al. (2005) J Biol. Chem.280, 21295-21312).
  • Superoxide is one form of reactive oxygen species (ROS).
  • ROS reactive oxygen species
  • Superoxide itself is not believed to react readily with biological molecules such lipid membranes, proteins and DNA, and actually functions as a signaling molecule for the regulation of certain cellular processes.
  • ROS reactive oxygen species
  • Hydroxyl radicals are veiy highly reactive, capable of reacting with virtually any biological molecule, including DNA, proteins and lipids. Hydroxyl radicals can also diffuse through cells readily, and their ability to damage cells is limited only by the distance that they travel before they react. Hydroxyl radicals can also react with superoxide, producing singlet oxygen (( ! 0 2 ) + OH " ), another highly reactive form of ROS that damages cellular macromolecules and assemblies.
  • One particularly deleterious and well-studied reaction mediated by hydroxyl radicals is the abstraction of hydrogen atoms (H e ) from membrane lipids, forming a carbon- centered radical (R e ). This radical
  • ROO' + RH ⁇ ROOH + R- can readily react with oxygen, forming a hydroperoxy radical (ROO-).
  • the hydroperoxy radical is also highly reactive, and can abstract another hydrogen atom from the membrane lipid, producing another carbon-centered radical (which can undergo precisely the same chemistry), ultimately producing a chain reaction affording many oxidative lesions in the membrane lipids from a single hydroxyl radical (lipid peroxidation). It is for this reason that lipid peroxidation likely represents a major process by which cellular and mitochondrial membranes are degraded in cells containing (partially) dysfunctional mitochondria.
  • One poorly studied parameter of mitochondrial electron transport is whether the process is best characterized as involving one or two electron transfers.
  • The is important because NADH is an obligatory two-electron donor, and coenzyme Q and cytochrome c participate in two-electron redox cycles, as does FADH 2 .
  • Virtually all publications represent the processes in which these species participate as involving a net two electron change.
  • FAD3 ⁇ 4 may (and generally does) transfer its reducing equivalents as single electrons.
  • the Q cycle in complex III clearly involves single-electron transfers.
  • Reduced cytochrome c is known to transfer electrons one at a time to cytochrome c oxidase, the enzyme responsible for the final step in respiration.
  • reductive stress build-up of electrons encountered initially in mitochondrial dysfunction is a one electron process, as is lipid peroxidation
  • carriers of single electrons could find utility in dealing with reductive stress, e.g. molecules in which the one- electron reduced intermediate is stabilized by dipole interactions, substituent effects, resonance effects or captodative effects.
  • Molecules designed to traffic single electrons, and which can (i) accept electrons from superoxide (ii) donate electrons to complex III and (iii) quench carbon-centered lipid radicals are especially useful.
  • Multifunctional Radical Quenchers (MRQs) of the invention can effectively protect mitochondria, cells and organisms from oxidative stress.
  • compositions comprising one or more of compounds as described above with respect to any of formulae (I)-(III) and an appropriate carrier, excipient or diluent.
  • carrier, excipient or diluent will depend upon the desired use for the composition, and may range from being suitable or acceptable for veterinary uses to being suitable or acceptable for human use.
  • the composition may optionally include one or more additional compounds.
  • the compounds described herein may be administered singly, as mixtures of one or more compounds or in mixture or combination with other agents useful for treating such diseases and/or the symptoms associated with such diseases.
  • the compounds may also be administered in mixture or in combination with agents useful to treat other disorders or maladies, such as steroids, membrane stabilizers, 5LO inhibitors, leukotriene synthesis and receptor inhibitors, mhibitors of IgE isotype switching or IgE synthesis, IgGisotype switching or IgG synthesis, ⁇ -agonists, tryptase inhibitors, aspirin, COX inhibitors, methotrexate, anti-TNF drugs, retuxin, PD4 inhibitors, p38 mhibitors, PDE4 inhibitors, and antiliistamines, to name a few.
  • the compounds may be administered in the form of compounds per se, or as pharmaceutical compositions comprising a compound.
  • compositions comprising the compound(s) may be manufactured by means of conventional mixing, dissolving, granulating, dragee-making levigating, emulsifying, encapsulating,- entrapping or lyophilization processes.
  • the compositions may be formulated in conventional manner using one or more physiologically acceptable carriers, diluents, excipients or auxiliaries which facilitate processing of the compounds into preparations which can be used pharmaceutically.
  • the compounds may be formulated in the pharmaceutical composition per se, or in the form of a hydrate, solvate, N-oxide or pharmaceutically acceptable salt, as previously described.
  • such salts are more soluble in aqueous solutions than the corresponding free acids and bases, but salts having lower solubility than the corresponding free acids and bases may also be formed.
  • compositions may take a form suitable for virtually any mode of administration, including, for example, topical, ocular, oral, buccal, systemic, nasal, injection, transdermal, rectal, vaginal, etc., or a form suitable for administration by inhalation or insufflation.
  • the compound(s) may be formulated as solutions, gels, ointments, creams, suspensions, etc. as are well-known in the ait.
  • Systemic formulations include those designed for administration by injection, e.g., subcutaneous, intravenous, intramuscular, intrathecal or intraperitoneal injection; as well as those designed for transdermal, transmucosal oral or pulmonary administration.
  • Useful injectable preparations include sterile suspensions, solutions or emulsions of the active compound(s) in aqueous or oily vehicles.
  • the compositions may also contain formulating agents, such as suspending, stabilizing and/or dispersing agent.
  • the formulations for injection may be presented in unit dosage form, e.g., in ampules or in multidose containers, and may contain added preservatives.
  • the injectable formulation may be provided in powder form for reconstitution with a suitable vehicle, including but not limited to sterile pyrogen free water, buffer, dextrose solution, etc., before use.
  • the active compound(s) may be dried by any art-known teclinique, such as lyopl ilization, and reconstituted prior to use.
  • penetrants appropriate to the barrier to be permeated are used in the formulation.
  • penetrants are known in the art.
  • the pharmaceutical compositions may take the form of, for example, lozenges, tablets or capsules prepared by conventional means with pharmaceutically acceptable excipients such as binding agents (e.g., pregelatinised maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose); fillers (e.g., lactose, microcrystalline cellulose or calcium hydrogen phosphate); lubricants (e.g., magnesium stearate, talc or silica); disintegrants (e.g., potato starch or sodium starch glycolate); or wetting agents (e.g., sodium lauryl sulfate).
  • binding agents e.g., pregelatinised maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose
  • fillers e.g., lactose, microcrystalline cellulose or calcium hydrogen phosphate
  • lubricants e.g., magnesium stearate, talc or silica
  • disintegrants e.g
  • Liquid preparations for oral administration may take the form of, for example, elixirs, solutions, syrups or suspensions, or they may be presented as a dry product for constitution with water or other suitable vehicle before use.
  • Such liquid preparations may be prepared by conventional means with pharmaceutically acceptable additives such as suspending agents (e.g., sorbitol syrup, cellulose derivatives or hydrogenated edible fats); emulsifying agents (e.g., lecithin or acacia); non-aqueous vehicles (e.g., almond oil, oily esters, ethyl alcohol, cremophoreTM or fractionated vegetable oils); and preservatives (e.g., methyl or propyl-p-hydroxybenzoates or sorbic acid).
  • the preparations may also contain buffer salts, preservatives, flavoring, coloring and sweetening agents as appropriate.
  • Preparations for oral administration may be suitably formulated to give controlled release of the compound, as is well known.
  • compositions may take the form of tablets or lozenges formulated in conventional manner.
  • the c mpound(s) may be formulated as solutions (for retention enemas) suppositories or ointments containing conventional suppository bases such as cocoa butter or other glycerides.
  • the compound(s) can be conveniently delivered in the form of an aerosol spray from pressurized packs or a nebulizer with the use of a suitable propellant, e.g., dichlorodifluoromethane, trichloiOfluoromethane, dichlorotetrafluoroethane, fluorocarbons, carbon dioxide or other suitable gas.
  • a suitable propellant e.g., dichlorodifluoromethane, trichloiOfluoromethane, dichlorotetrafluoroethane, fluorocarbons, carbon dioxide or other suitable gas.
  • the dosage unit may be determined by providing a valve to deliver a metered amount.
  • Capsules and cartridges for use in an inhaler or insufflator may be formulated containing a powder mix of the compound and a suitable powder base such as lactose or starch.
  • the compound(s) may be formulated as a solution, emulsion, suspension, etc. suitable for administration to the eye.
  • a variety of vehicles suitable for administering compounds to the eye are known in the art.
  • the compound(s) can be formulated as a depot preparation for administration by implantation or intramuscular injection.
  • the compound(s) may be formulated with suitable polymeric or hydrophobic materials (e.g., as an emulsion in an acceptable oil) or ion exchange resins, or as sparingly soluble derivatives, e.g., as a sparingly soluble salt.
  • suitable polymeric or hydrophobic materials e.g., as an emulsion in an acceptable oil
  • ion exchange resins e.g., as sparingly soluble derivatives, e.g., as a sparingly soluble salt.
  • transdermal delivery systems manufactured as an adhesive disc or patch which slowly releases the compound(s) for percutaneous absorption may be used.
  • permeation enhancers may be used to facilitate transdermal penetration of the compound(s).
  • Liposomes and emulsions are well-known examples of delivery vehicles that may be used to deliver compound(s).
  • Certain organic solvents such as dimethylsulfoxide (DMSO) may also be employed, although usually at the cost of greater toxicity.
  • DMSO dimethylsulfoxide
  • compositions may, if desired, be presented in a pack or dispenser device which may contain one or more unit dosage forms containing the compound(s).
  • the pack may, for example, comprise metal or plastic foil, such as a blister pack.
  • the pack or dispenser device may be accompanied by instructions for administration.
  • the compound(s) described herein, or compositions thereof will generally be used in an amount effective to achieve the intended result, for example in an amount effective to treat or prevent the particular disease being treated.
  • therapeutic benefit is meant eradication or amelioration of the underlying disorder being treated and/or eradication or amelioration of one or more of the symptoms associated with the underlying disorder such that the patient reports an improvement in feeling or condition, notwithstanding that the patient may still be afflicted with the underlying disorder.
  • Therapeutic benefit also generally includes halting or slowing the progression of the disease, regardless of whether improvement is realized.
  • the amount of compound(s) administered will depend upon a variety of factors, including, for example, the particular indication being treated, the mode of administration, whether the desired benefit is prophylactic or therapeutic, the severity of the indication being treated and the age and weight of the patient, the bioavailability of the particular compound(s) the conversation rate and efficiency into active drug compound under the selected route of administration, etc.
  • Effective dosages may be estimated initially from in vitro activity and metabolism assays.
  • an initial dosage of compound for use in animals may be formulated to achieve a circulating blood or serum concentration of the metabolite active compound that is at or above an ICs 0 of the particular compound as measured in as in vitro assay.
  • Calculating dosages to acliieve such circulating blood or serum concentrations talcing into account the bioavailability of the particular compound via the desired route of administration is well within the capabilities of skilled artisans.
  • Initial dosages of compound can also be estimated from in vivo data, such as animal models.
  • Animal models useful for testing the efficacy of the active metabolites to treat or prevent the various diseases described above are well-known in the art Animal models suitable for testing the bioavailability and/or metabolism of compounds into active metabolites are also well-known. Ordinarily skilled artisans can routinely adapt such information to determine dosages of particular compounds suitable for human administration.
  • Dosage amounts will typically be in the range of from about 0.0001 mg/kg/day, 0.001 mg/kg/day or 0.01 mg/kg/day to about 100 mg/kg/day, but may be higher or lower, depending upon, among other factors, the activity of the active metabolite compound, the bioavailability of the compound, its metabolism kinetics and other pharmacokinetic properties, the mode of administration and various other factors, discussed above. Dosage amount and interval may be adjusted individually to provide plasma levels of the compound(s) and/or active metabolite compound(s) which are sufficient to maintain therapeutic or prophylactic effect.
  • the compounds may be administered once per week, several times per week (e.g., every other day), once per day or multiple times per day, depending upon, among other things, the mode of administration, the specific indication being treated and the judgment of the prescribing physician.
  • the effective local concentration of compound(s) and/or active metabolite compound(s) may not be related to plasma concentration. Skilled artisans will be able to optimize effective local dosages without undue experimentation.
  • alkenyl as used herein, means a straight or branched chain hydrocarbon containing from 2 to 10 carbons, unless otherwise specified, and containing at least one carbon-carbon double bond.
  • Representative examples of alkenyl include, but are not limited to, ethenyl, 2-propenyl, 2-methyl-2-propenyl, 3-butenyl, 4-pentenyl, 5-hexenyl, 2-heptenyl, 2-methyl-l-heptenyl, 3-decenyl, and 3,7-dimethylocta-2,6-dienyl.
  • alkoxy as used herein, means an alkyl group, as defined herein, appended to the parent molecular moiety through an oxygen atom.
  • Representative examples of alkoxy include, but are not limited to, methoxy, ethoxy, propoxy, 2-propoxy, butoxy, tert-butoxy, pentyloxy, and hexyloxy.
  • alkyl as used herein, means a straight or branched chain hydrocarbon containing from 1 to 10 carbon atoms unless otherwise specified.
  • Representative examples of alkyl include, but are not limited to, methyl, ethyl, n-propyl, iso-propyl, n-butyl, sec-butyl, iso-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, n-hexyl, 3-methylhexyl, 2,2- dimethylpentyl, 2,3-dimethylpentyl, n-heptyl, n-octyl, n-nonyl, and n-decyl.
  • an “alkyl” group is a linking group between two other moieties, then it may also be a straight or branched chain; examples include, but are not limited to -CH 2 -, -CH 2 CH 2 - , -CH 2 CH 2 CHC(C3 ⁇ 4)-, -CH 2 CH(CH 2 CH 3 )CH 2 -.
  • alkynyl as used herein, means a straight or branched chain hydrocarbon group containing from 2 to 10 carbon atoms and containing at least one carbon-carbon triple bond. Representative examples of alkynyl include, but are not limited, to acetylenyl, 1 - propynyl, 2-propynyl, 3-butynyl, 2-pentynyl, and 1-butynyl.
  • aryl as used herein, means a phenyl (z. e. , monocyclic aryl), or a bicyclic ring system containing at least one phenyl ring or an aromatic bicyclic ring containing only carbon atoms in the aromatic bicyclic ring system.
  • the bicyclic aryl can be azulenyl, naphthyl, or a phenyl fused to a monocyclic cycloalkyl, a monocyclic cycloalkenyl, or a monocyclic heterocyclyl.
  • the bicyclic aryl is attached to the parent molecular moiety through any carbon atom contained within the phenyl portion of the bicyclic system, or any carbon atom with the napthyl or azulenyl ring.
  • the fused monocyclic cycloalkyl or monocyclic heterocyclyl portions of the bicyclic aryl are optionally substituted with one or two oxo and/or thia groups.
  • bicyclic aryls include, but are not limited to, azulenyl, naphthyl, dihydroinden-l-yl, dihydroinden-2-yl, dihydroinden-3-yl, dihydroinden-4-yl, 2,3-dihydroindol-4-yl, 2,3-dihydroindol-5-yl, 2,3-dihydroindol-6-yl, 2,3- dihydi indol-7-yl, inden-l-yl, inden-2-yl, inden-3-yl, inden-4-yl, dihydronaphthalen-2-yl, dihydronaphthalen-3-yl, dihydronaphthalen-4-yl, dihydronaphthalen-l-yl, 5,6,7,8- tetral ydronaphthalen- 1 -yl, 5 ,6,7, 8-tetraliydronaphthalen-2-
  • the bicyclic aryl is (i) naphthyl or (ii) a phenyl ring fused to either a 5 or 6 membered monocyclic cycloalkyl, a 5 or 6 membered monocyclic cycloalkenyl, or a 5 or 6 membered monocyclic heterocyclyl, wherein the fused cycloalkyl, cycloalkenyl, and heterocyclyl groups are optionally substituted with one or two groups which are independently oxo or thia.
  • cyano and “nitrile” as used herein mean a -CN group.
  • cycloalkyl as used herein, means a monocyclic or a bicyclic cycloalkyl ring system. Monocyclic ring systems are cyclic hydrocarbon groups containing from 3 to 8 carbon atoms, where such groups can be saturated or unsaturated, but not aromatic. In certain embodiments, cycloalkyl groups are fully saturated. Examples of monocyclic cycloalkyls include cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl, cycloheptyl, and cyclooctyl.
  • Bicyclic cycloalkyl ring systems are bridged monocyclic rings or fused bicyclic rings.
  • Bridged monocyclic rings contain a monocyclic cycloalkyl ring where two non-adjacent carbon atoms of the monocyclic ring are linked by an alkylene bridge of between one and three additional carbon atoms (z. e. , a bridging group of the form -(CH 2 ) W -, where w is 1, 2, or 3).
  • bicyclic ring systems include, but are not limited to, bicyclo[3.1.1]heptane, bicyclo[2.2.1]heptane, bicyclo[2.2.2]octane, bicyclo[3.2.2] nonane, bicyclo[3.3.1]nonane, and bicyclo[4.2.1]nonane.
  • Fused bicyclic cycloalkyl ring systems contain a monocyclic cycloalkyl ring fused to either a phenyl, a monocyclic cycloalkyl, a monocyclic cycloalkenyl, a monocyclic heterocyclyl, or a monocyclic heteroaryl.
  • the bridged or fused bicyclic cycloalkyl is attached to the parent molecular moiety through any carbon atom contained within the monocyclic cycloalkyl ring.
  • Cycloalkyl groups are optionally substituted with one or two groups which are independently oxo or thia.
  • the fused bicyclic cycloalkyl is a 5 or 6 membered monocyclic cycloalkyl ring fused to either a phenyl ring, a 5 or 6 membered monocyclic cycloalkyl, a 5 or 6 membered monocyclic cycloalkenyl, a 5 or 6 membered monocyclic heterocyclyl, or a 5 or 6 membered monocyclic heteroaryl, wherein the fused bicyclic cycloalkyl is optionally substituted by one or two groups which are independently oxo or thia.
  • halo or halogen as used herein, means -CI, -Br, -I or -F.
  • haloalkyl as used herein, means at least one halogen, as defined herein, appended to the parent molecular moiety through an alkyl group, as defined herein.
  • Representative examples of haloalkyl include, but are not limited to, chloromethyl, 2- fluoroethyl, trifluoromethyl, pentafluoroethyl, and 2-chloiO-3-fluoropentyl.
  • heteroaryl means a monocyclic heteroaryl or a bicyclic ring system containing at least one heteroaromatic ring.
  • the monocyclic heteroaryl can be a 5 or 6 membered ring.
  • the 5 membered ring consists of two double bonds and one, two, three or four nitrogen atoms and optionally one oxygen or sulfur atom.
  • the 6 membered ring consists of three double bonds and one, two, three or four nitrogen atoms.
  • the 5 or 6 membered heteroaryl is connected to the parent molecular moiety through any carbon atom or any nitrogen atom contained within the heteroaryl.
  • monocyclic heteroaryl include, but are not limited to, furyl, imidazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, oxazolyl, pyridinyl, pyridazinyl, pyrimidinyl, pyrazinyl, pyrazolyl, pyixolyl, tetrazolyl, thiadiazolyl, thiazolyl, thienyl, triazolyl, and triazinyl.
  • the bicyclic heteroaryl consists of a monocyclic heteroaryl fused to a phenyl, a monocyclic cycloalkyl, a monocyclic cycloalkenyl, a monocyclic heterocyclyl, or a monocyclic heteroaryl.
  • the fused cycloalkyl or heterocyclyl portion of the bicyclic heteroaryl group is optionally substituted with one or two groups which are independently oxo or thia.
  • the bicyclic heteroaryl contains a fused cycloalkyl, cycloalkenyl, or heterocyclyl ring
  • the bicyclic heteroaryl group is connected to the parent molecular moiety through any carbon or nitrogen atom contained within the monocyclic heteroaryl portion of the bicyclic ring system.
  • the bicyclic heteroaryl is a monocyclic heteroaryl fused to a phenyl ring
  • the bicyclic heteroaryl group is connected to the parent molecular moiety through any carbon atom or nitrogen atom within the bicyclic ring system.
  • bicyclic heteroaryl include, but are not limited to, benzimidazolyl, benzofuranyl, benzothienyl, benzoxadiazolyl, benzoxathiadiazolyl, benzothiazolyl, cinnolinyl, 5,6-dihydroquinolin-2-yl, 5,6-dihydroisoquinolin-l-yl,, furopyridinyl, indazolyl, indolyl, isoquinolinyl, naphthyridinyl, quinolinyl, purinyl, 5,6,7,8- tetrahydroquinolin-2-yl, 5,6,7,8-tetrahydroquinolin-3-yl, 5,6,7,8-tetraliydroquinolin-4-yl, 5,6,7,8-tetrahydiOisoquinolin-l-yl, thienopyridinyl, 4,5, 6,7-
  • the fused bicyclic heteroaryl is a 5 or 6 membered monocyclic heteroaryl ring fused to either a phenyl . ring, a 5 or 6 membered monocyclic cycloalkyl, a 5 or 6 membered monocyclic cycloalkenyl, a 5 or 6 membered monocyclic heterocyclyl, or a 5 or 6 membered monocyclic heteroaryl, wherein the fused cycloalkyl, cycloalkenyl, and heterocyclyl groups are optionally substituted with one or two groups which are independently oxo or thia.
  • heterocyclyl as used herein, means a monocyclic heterocycle or a bicyclic heterocycle.
  • the monocyclic heterocycle is a 3, 4, 5, 6 or 7 membered ring containing at least one heteroatom independently selected from the group consisting of O, N, and S where the ring is saturated or unsaturated, but not aromatic.
  • the 3 or 4 membered ring contains 1 heteroatom selected from the group consisting of O, N and S.
  • the 5 membered ring can contain zero or one double bond and one, two or three heteroatoms selected from the group consisting of O, N and S.
  • the 6 or 7 membered ring contains zero, one or two double bonds and one, two or three heteroatoms selected from the group consisting of O, N and S.
  • the monocyclic heterocycle is connected to the parent molecular moiety through any carbon atom or any nitrogen atom contained within the monocyclic heterocycle.
  • Representative examples of monocyclic heterocycle include, but are not limited to, azetidinyl, azepanyl, aziridinyl, diazepanyl, 1,3-dioxanyl, 1,3-dioxolanyl, 1,3-dithiolanyl, 1,3-dithianyl, imidazolinyl, imidazolidinyl, isothiazolinyl, isothiazolidinyl, isoxazolinyl, isoxazolidinyl, morpholinyl, oxadiazolinyl, oxadiazolidinyl, oxazolinyl, oxazolidinyl, piperazinyl, piperidinyl, pyranyl, pyrazolinyl, pyrazolidinyl, pyr
  • the bicyclic heterocycle is a monocyclic heterocycle fused to either a phenyl, a monocyclic cycloalkyl, a monocyclic cycloalkenyl, a monocyclic heterocycle, or a monocyclic heteroaryl.
  • the bicyclic heterocycle is connected to the parent molecular moiety tlirough any carbon atom or any nitrogen atom contained within the monocyclic heterocycle portion of the bicyclic ring system.
  • bicyclic heterocyclyls include, but are not limited to, 2,3- dihydrobenzofurai -2-yl, 2,3-dihydi benzofuran-3-yl, indolin-l-yl, indolin-2-yl, indolin-3-yl, 2,3-dihydrobenzothien-2-yl, decahydroquinolinyl, decahydiOisoquinolinyl, octahydro-lH- indolyl, and octahydrobenzoforanyl.
  • Heterocyclyl groups are optionally substituted with one or two groups which are independently oxo or thia.
  • the bicyclic heterocyclyl is a 5 or 6 membered monocyclic heterocyclyl ring fused to phenyl ring, a 5 or 6 membered monocyclic cycloalkyl, a 5 or 6 membered monocyclic cycloalkenyl, a 5 or 6 membered monocyclic heterocyclyl, or a 5 or 6 membered monocyclic heteroaryl, wherein the bicyclic heterocyclyl is optionally substituted by one or two groups which are independently oxo or thia.
  • nitro as used herein, means a -N0 2 group.
  • saturated means the referenced chemical structure does not contain any multiple carbon-carbon bonds.
  • a saturated cycloalkyl group as defined herein includes cyclohexyl, cyclopropyl, and the like.
  • unsaturated means the referenced chemical structure contains at least one multiple carbon-carbon bond, but is not aromatic.
  • a unsaturated cycloalkyl group as defined herein includes cyclohexenyl, cyclopentenyl, cyclohexadienyl, and the like.
  • “Pharmaceutically acceptable” refers to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for contact with the tissues of human beings and animals without excessive toxicity, imtation, allergic response, or other problems or complications commensurate with a reasonable benefit/risk ratio or which have otherwise been approved by the United States Food and Drug Administration as being acceptable for use in humans or domestic animals.
  • “Pharmaceutically acceptable salt” refers to both acid and base addition salts.
  • “Therapeutically effective amount” refers to that amount of a compound which, when administered to a subject, is sufficient to effect treatment for a disease or disorder described herein.
  • the amount of a compound which constitutes 'a “therapeutically effective amount” will vary depending on the compound, the disorder and its seventy, and the age of the subject to be treated, but can be determined routinely by one of ordinary skill in the art.
  • Modulating or modulate refers to the treating, prevention, suppression, enhancement or induction of a function, condition or disorder.
  • the compounds of the present invention can modulate atherosclerosis by stimulating the removal of cholesterol from atherosclerotic lesions in a human.
  • Treating covers the treatment of a disease or disorder described herein, in a subject, preferably a human, and includes:
  • Subject refers to a warm blooded animal such as a mammal, preferably a human, or a human child, which is afflicted with, or has the potential to be afflicted with one or more diseases and disorders described herein.
  • EC50 refers to a dosage, concentration or amount of a particular test compound that elicits a dose-dependent response at 50% of maximal expression of a particular response that is induced, provoked or potentiated by the particular test compound.
  • IC 5 o refers to an amount, concentration or dosage of a particular test compound that achieves a 50% inhibition of a maximal response in an assay that measures such response.
  • Compounds as described herein can be purified by any of the means known in the art, including chromatographic means, such as HPLC, preparative thin layer chromatography, flash column chromatography and ion exchange chromatography. Any suitable stationary phase can be used, including normal and reversed phases as well as ionic resins. Most typically the disclosed compounds are purified via silica gel and/or alumina chromatography. See, e.g., Introduction to Modem Liquid Chromatography, 2nd Edition, ed. L. R. Snyder and J. J. Kirkland, John Wiley and Sons, 1979; and Thin Layer Chromatography, ed E. Stahl, Springer-Veiiag, New York, 1969.
  • reaction mixture was quenched by the addition of 10 mL of sat aq NH 4 C1 at 0 °C.
  • the mixture was extracted with EtOAc.
  • the combined organic layer was washed with brine, dried (MgS0 4 ) and concentrated under diminished pressure.
  • the residue was purified by flash chromatography on a silica gel column (25 3.2 cm).
  • Linear gradients were employed using 1 :4 methanol/water ⁇ 4:1 methanol/water over a period of 20 min, and then 4: 1 methanol/water ⁇ methanol over a period of 40 min, at a flow rate of 3.5 mL/min (monitoring at 260 nm).
  • reaction mixture was quenched by the addition of 10 mL of sat aq NH 4 C1 at 0 °C.
  • the mixture was extracted with EtOAc.
  • the combined organic layer was washed with brine, dried (MgS0 4 ) and concentrated under diminished pressure.
  • the residue was purified by flash chromatography on a silica gel column (25 3.2 cm).
  • Linear gradients were employed using 1 :4 methanol/water ⁇ 4:1 methanol/water over a period of 20 min, and then 4:1 methanol/water ⁇ methanol over a period of 40 min, at a flow rate of 3.5 mL/min (monitoring at 260 nm).
  • reaction mixture was quenched by the addition of 10 mL of sat aq NH 4 C1 at 0 °C.
  • the mixture was extracted with EtOAc.
  • the combined organic layer was washed with brine, dried (MgS0 4 ) and concentrated under diminished pressure.
  • the residue was purified by flash chromatography on a silica gel column (25 3.2 cm).
  • Linear gradients were employed using 1 :4 methanol/water ⁇ 4:1 methanol/water over a period of 20 min, and then 4:1 methanol/water ⁇ methanol over a period of 40 min, at a flow rate of 3.5 mL/min (monitoring at 260 nm).
  • Step 1 To a mixture containing 10 mL (10.28 g, 104 mmol) of N-methyl-2- pyrrolidinone and 10 mL (13:25 g, 105 mmol) of dimethyl sulfate was stirred and heated at 90 °C for 1.5 h, then allowed to cool to room temperature. A solution containing 25 mL of 25% methanolic sodium methoxide and 72 mL of methanol was added at -10 °C under Ar over a period of 1 h.
  • Step 2 To a solution containing 1.20 g (12.1 mmol) of 4-amino-3-penten-2-one in 8 mL of toluene was added 3.00 g (20.7 mmol) of 2,2-Dimethoxy-l-methylpyiTolidine. The reaction mixture was heated at reflux and stirred for 2 h, cooled to 90 °C, and then treated with 2.38 g (24.8 mmol) of t-BuONa and 2 mL of t-BuOH. The reaction mixture was stirred at 90 °C for another 16 h. The cooled reaction mixture was quenched by the addition of 10 mL of sat aq. NH4CI. The mixture was extracted with three 30-mL portions of EtOAc.
  • Step 3 To a solution containing -490 mg (2.71 mmol) of l,4,6-Trimethyl-2,3- dihydro-lH-pyrrolo[2,3-b]pyridine in 7.2 mL of THF was added 2.70 mL (4.34 mmol) of n- BuLi (1.6 M in hexanes) followed by 450 (509 mg, 2.85 mmol) of 1-bromoheptane at -78 °C. The reaction mixture was slowly warmed to room temperature and stirred for 16 h.
  • reaction mixture was quenched by the addition' of 25 mL of sat aq. NH 4 C1 at 0 °C.
  • the mixture was extracted with three 40-mL portions of EtO Ac.
  • the combined organic layer was washed with brine, dried over anhydrous MgS0 4 , filtered, and concentrated under diminished pressure.
  • the residue was purified via flash chromatography on a silica gel column (42 x 2.5 cm).
  • Step 4 To a solution containing 402 mg (1.54 mmol) of l,4-Dimethyl-6-octyl-2,3- dihydi -lH-pyrrolo[2,3-b]pyridine in 6 mL of chloroform was added 220 mg (0.77 mmol) of l,3-dibi mo-5,5-dimethylhydantoin in five portions at 0 °C. The reaction mixture was stirred at 0 °C for 30 min. The reaction mixture was quenched by the addition of 8 mL of sat aq. NaHC0 3 . The mixture was extracted with three 25-mL portions of chloroform.
  • Step 5 To a solution containing 202 mg (0.60 mmol) of 5-BiOmo-l,4-dimethyl-6- octyl-2,3-dihydi -lH-pyiTolo[2,3-b]pyridine in 5 mL of THF was added 90 ⁇ , (69.7 mg, 0.60 mmol) of tetramethyletl ylenediamine (TMEDA) at -78 °C followed by 0.75 mL (1.20 mmol) of n-BuLi (1.6 M in hexanes).
  • THF tetramethyletl ylenediamine
  • a widely used fluorescence assay for lipid peroxidation uses lipid soluble cw-parinaric acid as a probe, cis- parinaric acid loses its fluorescence ( ⁇ eX c/ em: 320/432 nm) upon interaction with peroxyl radicals and retains its fluorescence in the presence of radical quenchers, cw-parinaric acid is, however, air sensitive, photolabile and absorbs light in the UV region of the spectrum (at -320 nm). However, this region of the spectrum is where most compounds have also been found to absorb and emit light. In practical terms, the results obtained using cw-parinaric as a probe for lipid peroxidationare confounded due to the overlapping of the compounds emission spectra with the cis-parinaricemission spectrum.
  • Cii-BODIPY 581 591 oxidation to measure lipid peroxidation
  • a fluorescence assay for lipid peroxidation using a lipophilic probe belonging to the BODIPY class of fluorescent dyes was used.
  • C n -BODIPY 581/591 (4, 4-difluoro-5(4-phenyl-l, 3-butadienyl)-4- bora-3a, 4a-diaza-s-indacene-3 -propionic acid) fluorescence shifts from red to green upon oxidation.
  • Cn-BODIPY 581 591 (Molecular Probes, Eugene, ORE, USA) stock solution concentrations were determined by measuring the absorption of Cn-BODIPY 581/591 at 582 nm using a molar extinction coefficient of 140,000 mol "1 cm _1 (R.P. Haugland, (1999) Handbook of Fluorescent Probes and Research Chemicals, Molecular Probes, Inc., Eugene, 0R).
  • the lipid peroxidation inducer 2,2'-Azobis (2-amidino-propane dihydrochloride) (AAPH) and the antioxidant compound a-tocopherol(a-TOH) were obtained from Sigma (St. Louis, MO, USA).
  • Phospholipid bilayers were prepared from l-stearoyl-2-oleoyl-phosphatidylcholine (SOPC) and 1, 2-dilinoleoyl-phosphatidylcholine (DLPC) and were purchased from Avanti ® polar lipids, INC (Alabaster, AL, USA).
  • SOPC l-stearoyl-2-oleoyl-phosphatidylcholine
  • DLPC 2-dilinoleoyl-phosphatidylcholine
  • PC liposomes were prepared as described before (Guey- Shuangei al. ⁇ 2)Lipids.ll , 403-413). Briefly, DLPC (25 mg) and SOPC (25 mg) were dissolved in chloroform and the solvent was removed by nitrogen evaporation ( ⁇ 2 hours to give a thin film of PC in a round bottom flask. The lipid film was hydrated with 50 n L of 10 mM Tris-HCl (pH 7.4), 100 mM KC1, shaken and sonicated for 15 seconds. The liposomes obtained were filtered several times through 0.2 ⁇ membrane filter.
  • Cn BODIPY 581 591 was incorporated into liposomes and oxidized by peroxyl radicals derived from the decomposition of AAPH in presence and absence of the compounds.
  • Liposomes (1 mg/mL), suspended in 10 mM Tris-HCl (pH 7.4), 100 mM KC1, were transferred to a quartz 1 mL cuvette and placed in a Varian Cary Eclipse fluorometer (V arian, Cary, NC) equipped with a theiinostatted cuvette holder at 40 C. Liposomes were pre- incubated for 10 min with 200 nM C n BODIPY 581/591 to allow their incorporation into the lipid phase of the liposomes.
  • Lipid peroxidation in CEM leukemia cells was detected by utilizing the oxidant- sensitive lipophilic probe Cn BODIPY 581 591 . Briefly, CEM cells (5 x 10 5 cell/ mL) were treated with the test compounds at final concentrations of 2.5and 5 ⁇ , and incubated at 37 °C for 16 h in a humidified atmosphere containing 5% C0 2 in air. Cells were treated with 1 ⁇ BODIPY 581 591 in phenol red-free RPMI-1640 media and incubated at 37 °C in the dark for 30 minutes.
  • the cells were washed twice with phosphate buffered saline and oxidative stress was induced with 5 mM DEM in phenol red-free RPMI-1640 media for 90 minutes. Treated cells were collected by centrifugation at 300 x g for 3 minutes and then washed twice with phosphate buffered saline. Cells were re-suspended in 250 ⁇ , of phosphate buffered saline and were analyzed by FACS (FACS Calibur flow cytometer, Becton Dickinson) to monitor the change in intensity of the Cn BODIPY 581 591 -green (oxidized) fluorescence signal. A total of 10000 events were recorded for each sample and analyzed with the CellQuest software (BD Biosciences). The results were verified by repeating the experiments in duplicate. Results are expressed as the relative percentage of treated control (DEM).
  • Bovine heart mitochondria (1 mg protein) prepared as described by Smith (38) were added to 800 ⁇ ⁇ of 50 mM phosphate buffer, pH 8.0, and subjected to oxidative stress by the addition of 25 mM glucose and 1 U/mL glucose oxidase from Aspergillusniger. Samples were incubated with or without test compounds at 37 °C for 30 minutes.
  • TBARS were determined fluorometrically from the emission spectrum ( ⁇ ⁇ 515 nm; ⁇ ⁇ ⁇ 550 nm) using a Varian fluorimeter.
  • the malondialdehyde concentration was determined based on a standard curve created using serial dilutions of 10 mM 1,1,3,3-tetraethoxyproparie hydrolyzed in 1% (v/v) H 2 S0 4 at 4 °C overnight.
  • the malondialdehyde concentration was expressed as nmolesmanoldialdehyde per mg protein. Protein in aliquots of the homogenates was determined by the bicinchoninic acid method.
  • ROS Reactive Oxygen Species
  • DCFH-DA 2 ,7'-dichloi dihydiOfluorescein diacetate
  • DCFH-DA is a non-fluorescent derivative of fluorescein that emits fluorescence after being oxidized by hydrogen peroxide and other ROS. The emitted fluorescence is directly proportional to the concentration of hydrogen peroxide.
  • DCFH-DA is nonionic and nonpolar and is easily taken up by cells.
  • DCFH-DA is hydrolyzed by cellular esterases to non-fluorescent DCFH which traps the dye in the cell.
  • DCFH is oxidized to the highly fluorescent compound dichlorofluorescein (DCF).
  • DCF dichlorofluorescein
  • GSH glutathione
  • DEM chemical diethylmaleate
  • CEM cells (5x 10 5 cell/ mL) were plated (1 mL) in 24-well plate and treated with the invention compounds (final concentration 1, 2.5, and 5 ⁇ ), and incubated at 37 °C, 5% C0 2 for 16 hours.
  • the compounds tested were prepared by first making stock solutions (2.5 mM) in dimethylsulfoxide (DMSO). Cells were treated with 5 mM DEM for 50 minutes and collected by centrifugation at 300 x g for 3 min and then washed twice with Hanks' Balanced Salt Solution (HSSB) buffer (Sigma, St. Louis, MO. USA).
  • HSSB Hanks' Balanced Salt Solution
  • CEM leukemia cells were pre-treated with or without the test compounds for 16 h.
  • the cells were treated with 5 mM DEM for 120 minutes, collected by centrifugation at 300 x g for 3 minutes and then washed twice with phosphate buffered saline.
  • the cells were re-suspended in HSSB buffer and incubated at 37 °C in the dark for 15 minutes with 250 nM TMRM (a cationic dye which accumulates within mitochondria in accordance with the ⁇ ⁇ Nernst potential).
  • TMRM a cationic dye which accumulates within mitochondria in accordance with the ⁇ ⁇ Nernst potential
  • the samples were analyzed immediately by flow cytometry using 488 nm excitation laser and the FL2-H channel. The results obtained were verified in three independent experiments.
  • the protonophore FCCP (10 ⁇ ) was used to dissipate the chemio.smotic proton gradient ( ⁇ ) and served as a control for loss of ⁇ ⁇ . hi each analysis, 10,000 events were recorded and the percentage of cells exhibiting a high level of TMRM uptake, which reflects normal mitochondrial membrane potential ( ⁇ 1 ⁇ ), was determined and analyzed with the CellQuest software (BD Biosciences). The results were verified by repeating the experiments in duplicate.
  • Cell viability determined by trypan blue exclusion assay This technique was used to assess the cytoprotective effects of the invention compounds in cultured cells pharmacologically treated to induce cell death by GSH depletion. DEM was used to deplete cellular GSH and induce oxidative stress. The viability of DEM-treated CEM cells was determined by their ability to exclude the dye trypan blue. Viable cells exclude trypan blue; whereas, non-viable cells take up the dye and stain blue.
  • CEM lymphoblasts (CCL- 119) were grown in RPMI 1640 medium (GIBCO, Grand island, NY, USA) supplemented with 10% fetal calf serum, 2 mM glutamine (HyClone, South Logan, Utah, USA), 1% penicillin-streptomycin mix (Cellgro, Manassan, VA, USA). Cells were seeded at a density of 5x 10 5 cells/mL and treated with different concentrations of the invention compounds. Cells were incubated at 37 C ° in a humidified atmosphere of 5% C0 2 in air for 16 hours. After preincubation, the cells were treated with 5 mM DEM for 5.5 h.
  • Cell viability and cytotoxicity were determined by simultaneous staining live and dead cells using a two-color fluorescence assay, the Live/Dead ® Viability/Cytotoxicity Kit (Molecular Probes). This assay is used to measure two recognized parameters of cell viability, intracellular esterase activity and plasma integrity.
  • the membrane-impermeant DNA dye ethidium homodimer-1 (EtliD-1) was used to identify dead cells whose plasma membrane integrity was disrupted.
  • the membrane-permeant dye calcein-AM was used to label live cells. It penetrates into the cells where it is metabolized by cytoplasmic esterases and becomes a fluorescent but membrane-impermeant probe which is retained in viable cells.
  • FIG. 1 shows representative DCF overlay of FACS histograms of CEM cells stained with DCFH-DA and analyzed as described in the experimental section.
  • DEM treatment caused the DCF fluorescence (FL1-H) to shift right on the x-axis of the FACS histogram, indicating increased ROS production as a result of glutathione depletion.
  • Pretreatment of CEM cells with the ot-tocopherol with compound lb and 2b afforded significantly better protection than a-tocopherol, while la and 2a afforded reduced protection and lc and 2c gave intermediate protection.
  • FIG. 2 shows representative C n -BODIPY 581/591 - green (oxidized) FACS histograms overlay of CEM leukemia cells stained with BODIPY 581 591 - red (reduced) and analyzed using the FL1-H channel, as described in the experimental section.
  • DEM treatment caused the BODIPY 581 591 ⁇ green fluorescence to shift right on the x-axis of the FACS histogram, indicating increased membrane peroxidation as a result of glutathione depletion.
  • Pretreatment of CEM cells with lb afforded significantly better protection than was achieved with a-tocopherol, while 2b gave results comparable to a-TOH.
  • Figure 3 illustrates representative two-dimensional density dot plots of TMRM- stained cells showing the percentage of cells with intact ⁇ ⁇ (TMRM fluorescence in top right quadrant) vs the percentage of cells with depolarized ⁇ ⁇ (TMRM fluorescence in bottom left and right quadrants).
  • Figure 4 shows a bar graph of the percentage (mean ⁇ S.E.) of CEM cells with intact ⁇ ⁇ . The results show that DEM treatment reduced the percentage of cells with TMRM fluorescence in the top right quadrant, indicating that DEM treatment caused loss of ⁇ ⁇ .

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10364227B2 (en) 2015-02-17 2019-07-30 Arizona Board Of Regents On Behalf Of Arizona State University Therapeutic compounds
US10472340B2 (en) 2015-02-17 2019-11-12 Arizona Board Of Regents On Behalf Of Arizona State University Substituted phenothiazines as mitochondrial agents
US10604501B2 (en) 2016-06-15 2020-03-31 Arizona Board Of Regents On Behalf Of Arizona State University Prodrug and profluorescent compounds for selective mitochondrial imaging and therapeutic targeting
US10745366B2 (en) 2016-08-25 2020-08-18 Arizon Board Of Regents On Behalf Of Arizona State University Therapeutic compounds
US11390605B2 (en) 2016-08-25 2022-07-19 Arizona Board Of Regents On Behalf Of Arizona State University Substituted pyrimidine compounds as multifunctional radical quenchers and their uses

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CN112326607B (zh) * 2020-10-16 2022-06-28 暨南大学 一种低浓度ros的检测方法及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011103536A1 (en) * 2010-02-19 2011-08-25 Arizona Board Of Regents Multifunctional radical quenchers and their use

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3476772A (en) * 1966-10-20 1969-11-04 Eastman Kodak Co Chroman derivatives of 2,5-dimethyl-2,4-hexadiene
DE2315349A1 (de) * 1973-03-28 1974-10-03 Bayer Ag Nicht verfaerbende alterungsschutzmittel
JPS5168569A (en) * 1974-12-10 1976-06-14 Fuji Photo Film Co Ltd 2 2 44 torimechiru 66 hidorokishi 77 chikankuromanruino seizoho

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011103536A1 (en) * 2010-02-19 2011-08-25 Arizona Board Of Regents Multifunctional radical quenchers and their use

Non-Patent Citations (41)

* Cited by examiner, † Cited by third party
Title
"Introduction to Modern Liquid Chromatography", 1979, JOHN WILEY AND SONS
"The Peptides", 1981, ACADEMIC PRESS
"Thin Layer Chromatography", 1969, SPRINGER-VERLAG
BENCZE ET AL., J.C.S. CHEM. COMMUN., 2007, pages 1798 - 1800
BRADLEY ET AL., HUM. MOL. GENET., vol. 9, 2000, pages 275 - 282
BULTEAUET, SCIENCE, vol. 305, 2004, pages 242 - 245
CAI, X. ET AL.: "Simplified bicyclic pyridinol analogues protect mitochondrial function", BIOORGANIC & MEDICINAL CHEMISTRY, vol. 20, no. 11, 31 March 2012 (2012-03-31), pages 3584 - 3595, XP028423314, ISSN: 0968-0896, [retrieved on 20120413], DOI: 10.1016/J.BMC.2012.03.075 *
CAMPUZANO ET AL., HUM. MOL. GENET., vol. 6, 1997, pages 1771 - 1780
CAMPUZANO ET AL., SCIENCE, vol. 271, 1996, pages 1423 - 1427
DIMAURO ET AL., AM. J. MED. GENET., vol. 106, 2001, pages 18 - 26
DRUMMEN ET AL., FREE RADICBIOL MED, vol. 33, 2002, pages 473 - 490
GONZALEZ-CABOET, HUM. MOL. GENET., vol. 14, 2005, pages 2091 - 2098
GUEY-SHUANG ET AL., LIPIDS, vol. 17, 1982, pages 403 - 413
H.-D. JAKUBKE; H. JESCHEIT: "Aminosauren, Peptide, Proteine", 1982, VERLAG CHEMIE
HOUBEN-WEYL: "Methoden der organischen Chemie", vol. 15/1, 1974, GEORG THIEMEVERLAG
J. F. W. MCOMIE: "Protective Groups in Organic Chemistry", 1973, PLENUM PRESS
JAMES ET AL., JBIOL. CHEM., vol. 280, 2005, pages 21295 - 21312
KUYPERS ET AL., BIOCHIM BIOPHYS ACTA, vol. 25, 1987, pages 266 - 274
LA MARCHE ET AL., CAN. J NEUROSCI., vol. 7, 1980, pages 389 - 396
LEBEL ET AL., CHEM. RES. TOXICOL., vol. 5, 1992, pages 227 - 231
LEONARD ET AL., LANCET, vol. 355, 2000, pages 299 - 304
LU, J. ET AL.: "Concise Synthesis of Bicyclic Pyridinol Antioxidants", ORGANIC LETTERS, vol. 12, no. 22, 19 November 2010 (2010-11-19), pages 5189 - 5191, XP055060742, ISSN: 1523-7060, DOI: 10.1021/ol102217c *
LU, J. ET AL.: "Design, synthesis, and evaluation of an alpha-tocopherol analogue as a mitochondrial antioxidant", BIOORGANIC & MEDICINAL CHEMISTRY, vol. 18, no. 21, 2010, pages 7628 - 7638, XP027415417, ISSN: 0968-0896, [retrieved on 20100826] *
NAM, T. ET AL.: "Pyridoxine-derived bicyclic aminopyridinol antioxidants: synthesis and their antioxidant activities", ORGANIC & BIOMOLECULAR CHEMISTRY, vol. 9, no. 24, 22 September 2011 (2011-09-22), pages 8475 - 8482, XP055060741, ISSN: 1477-0520, DOI: 10.1039/c1ob05144j *
OCHEN LEHMANN: "Chemie der Kohlenhydrate: Monosaccharide and Derivate", 1974, GEORG THIEME VERLAG
PAP ET AL., FEBS LETT, vol. 453, 1999, pages 278 - 282
PARK ET AL., J BIOL. CHEM., vol. 278, 2003, pages 31340 - 31351
PARK ET AL., J. BIOL. CHEM., vol. 278, 2003, pages 31340 - 31351
R.P. HAUGLAND: "Handbook · of Fluorescent Probes and Research Chemicals", 1999, MOLECULAR PROBES, INC.
SMITH; MARCH: "March's Advanced Organic Chemistry: Reactions, Mechanisms, and Structure", 2001
T. W. GREENE; P. G. M. WUTS: "Protective Groups in Organic Synthesis", 1999, WILEY
VOGEL: "A Textbook of Practical Organic Chemistry, Including Qualitative Organic Analysis", 1978, LONGMAN
WIJTMANS, M. ET AL.: "6-AMINO-3-PYRIDINOLS: TOWARDS DIFFUSION-CONTROLLED CHAIN-BREAKING ANTIOXIDANTS", ANGEWANDTE CHEMIE. INTERNATIONAL EDITION, WILEY VCH VERLAG, WEINHEIM, vol. 42, no. 36, 22 September 2003 (2003-09-22), pages 4370 - 4373, XP009062481, ISSN: 1433-7851, DOI: 10.1002/ANIE.200351881 *
WIJTMANS, M. ET AL.: "SYNTHESIS AND REACTIVITY OF SOME 6-SUBSTITUTED-2,4-DIMETHYL-3-PYRIDINOLS, A NOVEL CLASS OF CHAIN-BREAKING ANTIOXIDANTS", JOURNAL OF ORGANIC CHEMISTRY, vol. 69, no. 26, 11 October 2004 (2004-10-11), pages 9215 - 9223, XP009062483, ISSN: 0022-3263, DOI: 10.1021/JO048842U *
WILSON ET AL., J NEUROL SCI., vol. 207, 2003, pages 103 - 105
WILSON ET AL., NAT. GENET., vol. 16, 1997, pages 352 - 357
YAMADA ET AL., J. LIPID RES., vol. 42, 2001, pages 1187 - 1196
YIN, D., FREE RAD. BIOL. MED., vol. 21, 1996, pages 871 - 888
YOON ET AL., J. AM CHEM. SOC., vol. 125, 2003, pages 6078 - 6084
YOON ET AL., J. AM. CHEM. SOC., vol. 125, 2003, pages 6078 - 6084
YOON ET AL., J. BIOL. CHEM., vol. 279, 2004, pages 25943 - 25946

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