WO2013091049A3 - Método para produção de plantas geneticamente modificadas com tolerância aumentada ao estresse salino e seca - Google Patents

Método para produção de plantas geneticamente modificadas com tolerância aumentada ao estresse salino e seca Download PDF

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Publication number
WO2013091049A3
WO2013091049A3 PCT/BR2012/000499 BR2012000499W WO2013091049A3 WO 2013091049 A3 WO2013091049 A3 WO 2013091049A3 BR 2012000499 W BR2012000499 W BR 2012000499W WO 2013091049 A3 WO2013091049 A3 WO 2013091049A3
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WO
WIPO (PCT)
Prior art keywords
abiotic
plants
drought
genetically modified
increased resistance
Prior art date
Application number
PCT/BR2012/000499
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English (en)
French (fr)
Other versions
WO2013091049A2 (pt
Inventor
Marcelo Menossi Teixeira
Cesar Bueno DE SOUZA
Andrea Akemi HOSHINO
Éder Bedani Ruis CHAGAS
Gláucia Mendes SOUZA
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Universidade Estadual De Campinas - Unicamp
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Application filed by Universidade Estadual De Campinas - Unicamp filed Critical Universidade Estadual De Campinas - Unicamp
Publication of WO2013091049A2 publication Critical patent/WO2013091049A2/pt
Publication of WO2013091049A3 publication Critical patent/WO2013091049A3/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/03—Hydrolases acting on acid anhydrides (3.6) acting on acid anhydrides; catalysing transmembrane movement of substances (3.6.3)
    • C12Y306/03014—H+-transporting two-sector ATPase (3.6.3.14), i.e. F1 ATPase

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

As plantas são influenciadas por um grande número de fatores ambientais bióticos e abióticos e recorrentemente estresses abióticos são mais graves, afetando todas as funções da planta, resultando em redução do crescimento e da produtividade. Nesse sentido, a identificação e a compreensão dos mecanismos de tolerância abióticos são fundamentais no desenvolvimento de novas cultivares tolerantes à seca. Dessa forma, a presente invenção descreve um método de produção de plantas que contém em suas células uma sequência de nucleotídeos de cana-de-açúcar. A expressão do vetor que contém a sequencia poderá levar a planta na qual ele é inserido à maior tolerância a estresses abióticos. De uma forma mais ampla, é descrito um polinucleotídeo que codifica um polipeptídeo de cana-de-açúcar, o qual é expresso por um promotor e um terminador que funcionam em plantas e este polinucleotídeo confere tolerância a diferentes estresses abióticos.
PCT/BR2012/000499 2011-12-22 2012-12-06 Polinucleotídeo de cana-de-açúcar que confere tolerância a estresses abióticos WO2013091049A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BRPI1105303-8 2011-12-22
BRPI1105303-8A BRPI1105303B1 (pt) 2011-12-22 2011-12-22 polinucleotídeo de cana-de-açúcar que confere tolerância a estresses abióticos

Publications (2)

Publication Number Publication Date
WO2013091049A2 WO2013091049A2 (pt) 2013-06-27
WO2013091049A3 true WO2013091049A3 (pt) 2013-11-07

Family

ID=48669623

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/BR2012/000499 WO2013091049A2 (pt) 2011-12-22 2012-12-06 Polinucleotídeo de cana-de-açúcar que confere tolerância a estresses abióticos

Country Status (2)

Country Link
BR (1) BRPI1105303B1 (pt)
WO (1) WO2013091049A2 (pt)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011188744A (ja) * 2010-03-11 2011-09-29 Tokyo Univ Of Science 高温耐性植物及びそのスクリーニング方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011188744A (ja) * 2010-03-11 2011-09-29 Tokyo Univ Of Science 高温耐性植物及びそのスクリーニング方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK 12 October 2008 (2008-10-12), accession no. U963772 *
NGAMHUI NI-ON ET AL.: "Differentially expressed proteins in sugarcane leaves in response to water deficit stress", PLANT OMICS JOURNAL, vol. 5, no. 4, June 2012 (2012-06-01), pages 365 - 371 *
ZHANG ET AL.: "Overexpressionofa mitochondrial ATP synthase small subunit gene (AtMrATP6) confers tolerance to several abiotic stress in Saccharomyces cerevisiae and Arapidopsis thaliana", BIOTECHNOL. LETT., vol. 30, 2008, pages 1289 - 1294 *
ZHOU GUI ET AL.: "Proteomic analysis of osmotic stress-responsive proteins in sugarcane leaves.", PLANT MOL. BIOL. REP, vol. 30, April 2012 (2012-04-01), pages 349 - 359 *

Also Published As

Publication number Publication date
WO2013091049A2 (pt) 2013-06-27
BRPI1105303A2 (pt) 2014-04-22
BRPI1105303B1 (pt) 2020-10-20

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