WO2013089481A1

WO2013089481A1 - Peristaltic pump, and regenerative cell extraction system using same

Info

Publication number: WO2013089481A1
Application number: PCT/KR2012/010906
Authority: WO
Inventors: 도병록; 이정규; 김지향; 박승훈; 신백수; 김철근; 김종훈
Original assignee: 주식회사 휴림바이오셀
Priority date: 2011-12-14
Filing date: 2012-12-14
Publication date: 2013-06-20
Also published as: KR101316575B1; KR20130067468A

Abstract

The present invention relates to a regenerative cell extraction system and to a peristaltic pump used in the system. The regenerative cell extraction system according to the present invention comprises: a first unit for isolating fatty tissue; a second unit for extracting stem cells; and a delivery unit for exchanging substances between the first unit and second unit and a plurality of storage bags and between the first unit and the second unit. The delivery unit is very uncomplicated and efficient so as to be easy to use. Also, the peristaltic pump according to the present invention uses a movable pressing member and a spring for resiliently pressing the pressing member, allows the use of all tubes of various thickness, and can make fluids move forwards by pressing a tube efficiently.

Description

Peristaltic Pump and Regenerated Cell Extraction System Using the Same

The present invention relates to a regenerative cell extraction system and a peristaltic pump used in the system. More specifically, the present invention relates to a system for extracting regenerative cells such as adipose derived stem cells by centrifugation of adipose tissue and fluids in a flexible tube. It relates to a peristaltic pump for pressurized conveyance.

Stem cells are defined as cells that possess clonogenic and self-renewal ability that can differentiate into multiple cell lines under certain conditions. Embryonic stem cells are derived from mammalian embryos at the blastocyst stage and possess the ability to differentiate into almost all cells present in the body, while adult stem cells are stem cells that are present in trace amounts in differentiated tissue after birth. It is a cell with the capacity of a cell.

Adult stem cells offer practical advantages over embryonic stem cells. In other words, adult stem cells, unlike embryonic stem cells, do not cause ethical problems and can be extracted from the patient. They are abundant in supply and inherent in various tissues of the human body. The most available sources of adult stem cells are bone marrow, peripheral blood, umbilical cord / umbilical cord blood and adipose tissue, as confirmed in recent studies. These cells can maintain, yield and replace the final differentiated cells in their own specific tissues as a result of tissue damage due to physiological cell turnover or wounds.

This ability, called cell plasticity, has led to the development of therapeutic applications aimed at regenerating defective tissues for the purpose of restoring the physiology and function of damaged organs. Adult stem cells are distributed not only in hematopoietic cells but also in various tissues, as known for decades, and as recently discovered, can be differentiated into blood vessels, muscles, bones, cartilage, skin, nerves, and the like. These cells are known as mesenchymal stem cells. In addition, platelet concentrates can be used to accelerate wound healing and, consequently, play a role in regenerative medicine that aids in the reconstitution of tissues such as bone, skin or other tissues.

Recently, adipose tissue has been found to contain large amounts of stem cells, progenitor cells and matrix materials suitable for therapeutic applications. Adipose tissue is also a rich source of vascular endothelial cells, which can contribute to tissue regeneration by promoting growth of renal blood vessels and stimulating stem and progenitor cell growth.

Many devices have been developed for collecting cells from adipose tissue, but these devices are not fully automated, such as inefficient extraction of adipose tissue, or some manual process from the collection of adipose tissue to the processing of tissue. not. In addition, conventional devices may not be completely sealed, which may cause problems such as contamination of tissues during the entire process from collection of adipose tissue to treatment.

Above all, the apparatus for extracting stem cells has a problem that it is not only complicated to install, but also inconvenient to use because a plurality of transport lines for transporting various materials are intricately intertwined. And, in the process of extracting stem cells from adipose tissue, such as discarded part of the effective extraction was not made.

Therefore, a simple line configuration of the device is not only easy to install and use, but also requires the development of a regenerative cell extraction system capable of increasing the yield by efficiently collecting the adipose tissue and processing the tissue.

In addition, peristaltic pumps are mainly used in regenerative cell extraction systems, and conventional peristaltic pumps have been defined in terms of tubes (lines) that can be used. That is, when the tube is thicker than the predetermined thickness, the use itself was impossible, and when thinner than the predetermined thickness, there was a problem in that the pumping efficiency was remarkably lowered because the tube could not be effectively pressurized.

The present invention is to solve the above problems, it is an object of the present invention to provide a peristaltic pump with improved pumping efficiency because it is possible to use all the tubes of various thicknesses without being able to transmit the pressure force to the tube.

In addition, in the present invention, two units for separating and extracting regenerated cells from tissues and a transfer system for interconnecting various storage bags are very simplified to facilitate use and installation, as well as to extract efficiency of regenerated cells from tissues. It is an object of the present invention to provide a regenerative cell extraction system whose structure is improved to be improved.

The peristaltic pump according to the present invention for achieving the above object, the base member; A rotating unit having a rotating plate rotatably installed at one side of the base member, a plurality of pressure rollers installed on the rotating plate to be spaced apart from each other along the circumferential direction of the rotating plate, and a motor for rotating the rotating plate; A moving member installed on the base member so as to reciprocate in a direction approaching and spaced apart from the rotating unit, and fixed at a predetermined point; The moving member is installed in the movable member so as to reciprocate in a direction approaching and spaced apart from the rotating unit, and an arc-shaped pressing surface is formed at one end thereof to press the elastically compressible and recoverable tube wound on the rotating unit. A pressing member pressurizing toward the roller; And a spring supported between the moving member and the pressing member to elastically press the pressing member toward the rotating unit.

Regeneration cell extraction system according to the present invention, for separating tissue (tissue), the sub-container is rotated to receive a rotational force from the outside and the space is formed to receive and separate the tissue, one end of the sub-container A first unit having a hollow entrance tube inserted into the sub container so as to be disposed below; A main container for extracting regenerated cells from the separation object transferred from the first unit, the main container is rotated by receiving a rotational force from the outside and the receiving portion is formed to accommodate the separation object, and one end is disposed below the main container A second unit having a hollow first discharge pipe inserted into the main container so that the other end thereof is disposed outside the main container; A transfer unit for exchanging fluid between the first unit, the second unit, and the plurality of reservoir bags, the transfer unit comprising a plurality of tubes elastically compressible and recoverable; And a plurality of peristetic pumps installed in the tube of the delivery unit to pressurize and transfer the fluid inside the tube.

The peristaltic pump according to the present invention has the advantage that it can be used universally because all the tubes of various thicknesses can be used.

In addition, the peristaltic pump can pressurize and support the tube by the spring, so that the pressing force of the pressure roller can be transmitted to the tube without loss, thereby improving the pumping efficiency.

On the other hand, the conventional stem cell extraction system was not easy to operate because several valves must be opened and closed together, but in the regenerated cell extraction system according to the present invention, only a connection line connected to the portion to be transferred need to be opened. As all other lines need to be closed, the operation is very simple and the process efficiency is improved.

In addition, in the present invention, since the target material, in particular, an aqueous solution containing stem cells may remain in the main transfer line to reduce the yield, the second unit may be introduced without introducing an air through the air inlet line without losing the aqueous solution including the stem cells. There is an advantage that can be transferred to.

1 is a schematic diagram of a regenerative cell extraction system according to an embodiment of the present invention.

FIG. 2 is a schematic cross-sectional view of the first unit shown in FIG. 1.

3 is a schematic exploded perspective view of the second unit shown in FIG. 1.

4 is a schematic longitudinal cross-sectional view of FIG. 3.

5 is a schematic perspective view of a pre-installation state of the valve shown in FIG. 1.

FIG. 6 is a schematic perspective view of the valve illustrated in FIG. 5.

FIG. 7 is a schematic cross-sectional view taken along the line a-a of FIG. 6.

FIG. 8 is a schematic cross-sectional view taken along the line b-b of FIG. 6.

9 is a schematic perspective view of the peristaltic pump shown in FIG. 1.

FIG. 10 is a schematic perspective view of an open state of the cover of the peristectec pump illustrated in FIG. 9.

FIG. 11 is a schematic cross-sectional view taken along the line c-c in FIG. 9. FIG.

12 is a schematic cross-sectional view taken along the line d-d of FIG. 9.

FIG. 13 is a schematic cross-sectional view taken along the line e-e of FIG. 9.

14 is a schematic cross-sectional view taken along the line f-f in FIG. 13.

FIG. 15 is a schematic cross-sectional view taken along the line g-g of FIG. 9.

The peristaltic pump according to the present invention for achieving the above object, the base member; A rotating unit rotatably installed on one side of the base member, a rotating unit including a plurality of pressure rollers installed on the rotating plate to be spaced apart from each other along the circumferential direction of the rotating plate, and a motor for rotating the rotating plate; Is installed in the base member so as to reciprocate in the direction approaching and spaced apart, the movable member which can be fixed in position at a certain point, is installed in the movable member so as to reciprocate along the direction approaching and spaced apart from the rotary unit, At one end, an arc-shaped pressing surface is formed to press the elastically compressible and restorable tube wound on the rotating unit toward the pressing roller, and is supported between the moving member and the pressing member to support the pressing member. It comprises a spring that elastically presses toward the rotating unit There is a characteristic.

According to the present invention, the moving member is formed with a through hole penetrating between the front and rear in the reciprocating direction of the moving member, the thread is formed on the outer peripheral surface, is inserted through the through hole of the moving member is pressed And a screw screwed into a screw hole formed in the member, wherein the spring is fitted to the screw.

In one embodiment of the present invention, coupled to at least one of both sides of the base member so as to be movable along the reciprocating direction of the pressing member, the insertion groove is formed in a direction crossing the reciprocating direction of the pressing member And a stopper installed in the movable member so as to reciprocate in a direction in which the insertion groove is formed, and a stopper elastically biased in the direction of insertion into the insertion groove. When inserted, the moving member is fixed in position.

In addition, a screw hole is formed on the side of the base member, the fixing member penetrates between one side and the other side and a long hole is formed along the moving direction of the fixing member, the screw is fastened to the screw hole through the long hole As a result, the fixing member may be fixed in position.

In one embodiment of the present invention, it is provided between the pressing roller of the rotary unit, the central portion is formed with a concave further provided with a guide roller that can be inserted into the tube.

On the other hand, the regenerated cell extraction system according to the present invention, for separating the tissue (tissue), the sub-container is formed by rotating the rotational force received from the outside and the space portion for receiving and separating the tissue, one end of the sub A first unit having a hollow entrance tube inserted into the sub container so as to be disposed below the container; A main container for extracting regenerated cells from the separation object transferred from the first unit, the main container is rotated by receiving a rotational force from the outside and the receiving portion is formed to accommodate the separation object, and one end is disposed below the main container A second unit having a hollow first discharge pipe inserted into the main container so that the other end thereof is disposed outside the main container; A transfer unit for exchanging fluid between the first unit, the second unit, and the plurality of reservoir bags, the transfer unit comprising a plurality of tubes elastically compressible and recoverable; And a plurality of peristetic pumps installed in the tube of the delivery unit to pressurize and transfer the fluid inside the tube.

The transfer unit may include a main transfer line connecting the access pipe of the first unit and the first discharge tube of the second unit, a plurality of connection lines branched from the main transfer line and each of which is connected to a storage bag at an end thereof. And an air inlet line branched from the main transfer line between the first unit and the plurality of connection lines, and equipped with an air filter at an end to filter external air, and installed in the connection line and the air inlet line, respectively. And a plurality of valves.

In addition, a waste storage bag is installed at an end of the connection line of any one of the connection lines, and is fixed at the front end of the connection line connected to the waste storage bag on the transport path of the fluid moved from the first unit to the second unit. A filter installed in the main conveying line to filter out agglomerates larger than a size, and a bypass branched from the main conveying line so that the fluid discharged from the first unit bypasses the filter and connected to a connection line in which the waste storage bag is installed. It further has a line.

Hereinafter, with reference to the accompanying drawings, it will be described in more detail with respect to the peristaltic pump and regenerative cell extraction system according to an embodiment of the present invention.

1 is a schematic configuration diagram of a regenerative cell extraction system according to an embodiment of the present invention, FIG. 2 is a schematic cross-sectional view of the first unit shown in FIG. 1, and FIG. 3 is a schematic view of the second unit shown in FIG. 1. 4 is an exploded perspective view and FIG. 4 is a schematic longitudinal cross-sectional view of FIG. 3.

1 to 4, the regenerated cell extraction system 1000 according to an embodiment of the present invention is the first unit 100, the second unit 200, the delivery unit 300 and a plurality of fermentative The pump 900 is provided with a peristaltic pump.

The regenerated cell extraction system 1000 is for separating tissues or separating tissues to extract regenerated cells such as stem cells. Tissues to be separated vary from adipose tissue to umbilical cord blood, etc. Hereinafter, fat tissue will be described as an example.

Referring to the drawings, the first unit 100 is for separating specific gravity by rotating and stirring cell tissues such as fat tissue, and includes a sub container 110 and an entrance tube 130.

The sub container 110 includes a sub container ball 111 and a sub container cap 112 coupled to an upper portion of the sub container ball 111, and a space portion 113 in which a fatty tissue can be accommodated. ) Is formed.

The lower part of the sub container ball 111 is formed in a shape of decreasing diameter toward the lower side, the groove portion 114 is formed concave at the lower end of the sub container ball 111.

The through hole 115 is formed in the center portion of the sub container cap 112, and the protruding wall portion 116 is formed to protrude upward from the outer circumference of the through hole 115.

In addition, the stirring blade 117 is formed on the inner wall of the lower portion of the sub container ball 111. The stirring blades 117 are formed to protrude toward the space portion 113 from the inner wall, and the four are arranged symmetrically at intervals of 90 degrees with respect to the center of rotation of the sub-container 110. Of course, there may be only one stirring blade 117, it is not necessarily symmetrical. The stirring blade 117 is rotated together in accordance with the rotation of the sub-container 110, serves to smoothly stir adipose tissue.

The entrance tube 130 functions as a flow path for transferring the washing liquid, the enzyme, or the like to the space 113 of the sub container 110, or for discharging the material in the sub container 110 to the outside. The entrance tube 130 is formed in a hollow shape and is inserted into the sub container 110 through the through hole 115 of the sub container 110. The lower end of the entrance tube 130 is disposed in the groove 114 of the sub container 110 in a state of being close to the bottom of the sub container 110, and the upper end of the entrance tube 130 is positioned above the sub container 110. Is placed.

In addition, in the present embodiment, an auxiliary pipe 140 is installed to communicate the inside and the outside of the sub container 110 in an independent path from the access pipe 130. The auxiliary pipe 140 functions as a passage through which air flows into and out of the sub container 110. Therefore, the auxiliary pipe 140 does not need to be inserted to the lower end of the sub container 110, and only needs to be inserted to the upper end of the sub container 110.

An interior of the auxiliary pipe 140, more precisely, a space between the inner circumferential surface of the auxiliary pipe 140 and the outer circumferential surface of the entrance pipe 130 forms a flow path 145 through which air can flow. However, the flow path 145 is not necessarily used only for the inflow and outflow of air, and may be used as the inflow and outflow path of various materials, such as to discharge substances such as blood contaminants or inject a washing solution, if necessary.

In addition, an insertion hole 135 is formed at an upper end of the entrance tube 130, and a stopper 136 is screwed to the insertion hole 135. When the stopper 136 is removed, a syringe needle (not shown) is inserted into the entrance tube 130 through the insertion hole 135 to inject the material into the sub container 110 or the groove of the sub container 110 as it is. The liquid substance placed in the 114 can be inhaled. When the syringe needle is not used, the insertion hole 135 is closed by the stopper 136 to prevent contamination of the space 113 inside the sub container 110.

And, the lower side of the auxiliary pipe 140, the shielding member 147 is fitted to the entrance pipe 130 is coupled. When the sub container 110 is rotated to separate the adipose tissue, a liquid substance such as blood or a washing solution may be rapidly raised to the upper part of the sub container 110, and these materials may be internally or auxiliary to the auxiliary pipe 140. It may be introduced into the through hole 115 between the tube 140 and the sub container 110. The shielding member 147 surrounds the through hole 115 and the auxiliary tube 140 to prevent blood contaminants from being introduced into the auxiliary tube 140. However, since the auxiliary pipe 140 must be through the space portion 113, the wrapping here is not meant to be completely sealed, and a gap d is formed at the upper portion so as to communicate with the space portion 113.

In addition, spiral outer auxiliary stirring blades (not shown) are formed on the outer circumferential surface of the entrance tube 130 to stir adipose tissue together with the lower stirring blades of the sub container 110.

On the other hand, the sub-container 110 should be rotated relative to the entrance tube 130 and the auxiliary pipe 140, the entrance tube 130 and the auxiliary pipe 140 and the sub container 110 is sealed to the sub container ( The space 113 of the 110 should be sealed.

In this embodiment, the compressive sealing member 150 and the friction member 160 are employed to allow the space 113 to be sealed while the sub container 110 is rotated. That is, the sealing member 150 employed in the present embodiment is formed in an annular shape and fitted to the protruding wall portion 116 of the sub container cap 112, and the flange portion 148 formed on the outer circumferential surface of the auxiliary pipe 140. It is interposed between the sub container caps 112. The sealing member 150 is made of an elastically compressible rubber material, and is compressed between the through hole 115 and the auxiliary pipe 140 when the sealing member 150 is compressed between the flange portion 148 of the auxiliary pipe 140 and the sub container cap 112. The gap between is completely sealable.

However, when the sealing member 150 is rotated together with the sub container 110, the rubber sealing member 150 has a high coefficient of friction and not only smooth rotation due to friction between the flange portion 148, but also high Friction heat is generated. The upper side of the sealing member 150 is attached to the friction member 160 of the material having a low coefficient of friction and high heat transfer coefficient. In this embodiment, the friction member 160 made of a ceramic material and formed in an annular shape is attached to the upper portion of the sealing member 150, and the friction member 160 is in close contact with the flange portion 148 of the auxiliary pipe 140. Surface contact.

However, in another embodiment, a retaining ring (not shown) and a bearing (not shown) may be used to achieve relative rotation and sealing between the second auxiliary pipe 140 and the sub container 110.

The second unit 200 will be described.

Referring to the drawings, the second unit 200 is primarily separated from the first unit 100 receives the aqueous solution containing the stem cells, for extracting the stem cells from the aqueous solution.

The second unit 200 performing the above function includes a main container 210, a first discharge pipe 220, and a second discharge pipe 230.

The main container 210 is provided with a main body 211 and a cover 212, the receiving portion 213 is formed to receive the separation object such as an aqueous solution containing adipose tissue or stem cells therein. The lower portion of the main body portion 211 is gradually formed narrower from the upper side to the lower side or is formed in a constant diameter, in particular, the recessed portion is formed in the lowermost end of the receiving portion 213.

A through hole 215 penetrating between an upper surface and a lower surface is formed in the center of the upper surface of the cover 210, and an annular outer wall portion 216 is formed to protrude upward from an outer circumference of the through hole 215.

In addition, the protruding accommodation portion 217 is formed convexly on the outside of the main container 210. That is, the protrusion accommodating part 217 is convex toward the outside of the main container 210 in the radial direction about the center of rotation of the main container 210 and is formed long along the height direction of the main container 210.

Also, in order not to affect the rotation of the main container 210, the protrusion accommodation portion 217 should be disposed symmetrically to the main container 210, and in this embodiment the center of rotation (c) of the main container 210 Four positions are arranged symmetrically at 90 ° angle intervals.

When the main container 210 is rotated, the separation object is separated by weight, and the heavy component is disposed outside in the main container 210 and the light component is disposed inside. Since the protrusion accommodating part 217 is disposed at the outermost side with respect to the center of rotation of the main container 210, the separation of the heaviest component is accommodated in the protrusion accommodating part 217. Stem cells isolated from adipose tissue are heavier than other components and thus are accommodated in the protruding portion 217 during centrifugation. This will be described later in detail.

On the other hand, the lower end of the protrusion receiving portion 217 is blocked by the blocking member 240. That is, stem cells should be accommodated in the protrusion accommodation portion 217 by centrifugation. If the lower end portion of the protrusion accommodation portion 217 is in communication with the bottom portion of the main container, the separation object may be separated before the centrifugation is properly performed. It is raised from the bottom of the container 210 to the protrusion receiving portion, and the stem cell attached to the protrusion receiving portion 217 may be lost as the aqueous solution hits up. In this way, the stem cells attached to the protrusion receiving portion 217 can be stably received, and the blocking member 240 is installed to prevent the loss of the stem cells.

The first discharge pipe 220 and the second discharge pipe 230 are both for communicating the receiving portion 213 of the main container 210 and the outside of the main container 210, the first discharge pipe 220 and the second Discharge pipe 230 forms a mutually independent path. The first discharge pipe 220 and the second discharge pipe 230 is disposed coaxially with the main container 210, the first discharge pipe 220 is formed long so that the lower end of the lower end of the main container 210, more precisely Although disposed in the groove portion 214, the second discharge pipe 230 is short and the difference is that the lower end is disposed in the upper end of the main container (210).

The material flowing through the first discharge pipe 220 and the second discharge pipe 230 may be changed according to the use type, but in the present embodiment is separated from the first unit 100 through the first discharge pipe 220. An aqueous solution and a washing solution containing stem cells may be introduced, and the stem cells or the washing solution extracted from the main container 210 may be discharged. In addition, the air may flow in and out through the second discharge pipe, and the cleaning solution after use may be discharged.

The interior of the second discharge pipe 230, more precisely, the space between the inner peripheral surface of the second discharge pipe 230 and the outer peripheral surface of the first discharge pipe 220 forms a flow path 235 through which fluid such as air, cleaning liquid can flow. do.

In addition, an insertion hole 237 is formed at an upper end of the first discharge pipe 220, and a cap 236 is screwed to the insertion hole 237.

On the other hand, the main container 210 should be rotated relative to the first discharge pipe 220 and the second discharge pipe 230, the sealing between the first discharge pipe 220 and the second discharge pipe 230 and the main container 210. Thus, the receiving portion 213 of the main container 210 should be sealed.

In this embodiment, the medium member 250 and the contact member 260 are employed to seal the receiving portion 213 while the main container 210 is rotated. That is, the intermediate member 250 employed in the present embodiment is formed in an annular shape and fitted to the outer wall portion 216 of the main container cover 212, and the flange portion 238 formed on the outer circumferential surface of the second discharge pipe 230. And the main container cover 212. The intermediate member 250 is made of an elastically compressible rubber material. When the intermediate member 250 is compressed between the flange portion 248 of the second discharge pipe 230 and the main container cover 212, the through hole 215 and the second discharge pipe 230 are formed. The gap between) is completely sealable.

However, when the intermediate member 250 is rotated together with the main container 210, the rubber intermediate member 250 has a high coefficient of friction, and thus the smooth rotation is not performed due to friction between the flange 248. Friction heat is generated. Accordingly, the contact member 260 of a material having a small friction coefficient and a high heat transfer coefficient is attached to the upper side of the intermediate member 250. In this embodiment, the contact member 260 made of a ceramic material and formed in an annular shape is attached to the upper portion of the intermediate member 250, and the contact member 260 is in close contact with the flange portion 248 of the second discharge pipe 230. Surface contact.

On the other hand, in the present embodiment, while the main container 210 is rotated for centrifugation, a structure capable of discharging the washing solution other than stem cells to the outside of the regenerated cell extraction unit 100 through the second discharge pipe 230. to provide. That is, in the present embodiment, the rotation of the main container 210 may be stopped and the cleaning liquid may be discharged from the lower portion of the main container 210 through the first discharge pipe 220, but the main container 210 may be rotated. The cleaning liquid may be discharged to the outside by the vowel member 270 and the guide member 280 which will be described later.

The vowel member 270 is fitted to the first discharge pipe 220, and is provided with a concave collecting portion 271 so as to temporarily receive the separation guided by the guide member 280 to be described later. In the present embodiment, the vowel member 270 is formed in a concave disc shape. The vowel member 270 is disposed above the main container 210, and more precisely, is disposed closely to the lower end of the second discharge pipe 230. In addition, the diameter of the vowel member 270 is set to a size such that the outer circumferential surface of the vowel member 270 may be close to the inner circumferential surface of the main container 210.

The guide member 280 is disposed on the vowel member 270 to guide the separator that is being rotated in the centrifuged state inside the main container 210 to the collection portion of the vowel member 270. In the present exemplary embodiment, the guide members 280 are formed to protrude upward from the upper surface of the vowel member 270, and four guide members 280 are symmetrically disposed at intervals of 90 ° on the vowel member 270. In addition, the plurality of guide member 280 is formed toward the center from the outer peripheral surface of the vowel member 270 is arranged to be curved in a curve. Accordingly, the plurality of guide members 280 disposed on the vowel member 270 are formed in a toroidal shape as a whole.

Of course, the guide member 280 is not necessarily arranged to be curved, it may be a straight line, it is not formed over the entire radius of the vowel member 270 to be formed as a short section in contact with the separation material It may be in various forms, such as. In addition, the number of guide members may be variously selected from one to a plurality.

The separation object accommodated in the main container 210 is rotated together while being pushed outward by centrifugal force as the main container 210 is rotated. As described above, the stem cells, which are heavy components, are placed on the protruding portion 217. It is accommodated, the light component such as the cleaning liquid maintains a state in close contact with the inner peripheral surface of the main container (210). When the main container starts to rotate, the separation object is rotated together to raise the water level from the bottom of the main container 210 to the upper part. The separated water whose level is increased to the top of the collection member 270 is in contact with the guide member 280. While interfering, the guide member 280 is guided toward the center of the vowel member 270. When a suction force is applied to the second discharge pipe 230 from the pump, the separation guide guided to the center portion of the collection member 270 is discharged to the outside through the second discharge pipe 230 by this suction force.

In addition, a plurality of distribution holes 272 are formed in the central portion of the vowel member 270, and the distribution holes 272 lower the pressure applied to the vowel member 270 and a large amount of the separator may be introduced rapidly. When the separator is prevented from overflowing.

Hereinafter, the delivery unit 300 will be described.

The transfer unit 300 includes a main transfer line 310, a connection line 321 to 326, an air inflow line 330, a measurement line 340, and a bypass line 350. These

lines

310, 321 to 326, 330, 340 and 350 are all made of a flexible tube that can be compressed by pressurization, through which materials (mainly fluids and aqueous solutions) can be moved.

The main transfer line 310 connects the access pipe 130 of the first unit 100 and the first discharge pipe 220 of the second unit 200 to each other.

The plurality of connection lines 321 to 326 are branched from the main transfer line 310 to be connected to the storage bags 391 to 396. The number of connection lines may vary depending on the usage conditions of the system 1000. In this embodiment, six connection lines are used. Specifically, the enzyme reservoir 391, the adipose tissue reservoir 392, the wash solution reservoir 393, the oil reservoir 394, the waste reservoir 395, and the regenerative cell (stem cell) reservoir 396. Equipped.

The important point is the arrangement order of the storage bags 391 to 396. The enzyme reservoir bag 391, the adipose tissue reservoir bag 392, and the oil reservoir bag 394 respectively transfer materials only between the first unit 100 and the first unit 100 in the main transfer line 310. Disposed in close proximity to the That is, the collagenase enzyme stored in the enzyme storage bag 391 is delivered only to the first unit 100, and the washed adipose tissue and oil discharged from the first unit 100 are each adipose tissue storage bag 392. And only to oil reservoir 394.

In addition, the regenerated cell storage bag 396 stores the regenerated cells discharged from the second unit 200, and thus, the regenerated cell storage bag 396 is disposed close to the second unit 200 in the main transfer line 310.

In addition, the washing liquid storage bag 393 and the waste storage bag 395 transfer the washing liquid to both the first unit 100 and the second unit 200. Thus, the wash liquor bag 393 and the waste bag 395 are located between the other reservoir bags exchanging material with only one

unit

100, 200, that is, the intermediate point between the first unit 100 and the second unit 200. Is placed on.

The air inflow line 330 is for introducing air into the main transport line 310 and is branched from the main transport line 310. An air filter 397 is mounted at an end of the air inflow line 330 to purify the air flowing into the main transport line 310.

Also important here is the arrangement position of the air inlet line (330). The air inflow line 330 branches from the main transport line 310 before the other connection lines 321 to 326 based on the transport path of the fluid moving from the first unit 100 to the second unit 200. do. That is, the first branch in the main transfer line 310.

The air inflow line 330 is to transfer the materials existing in the main transport line 310 to a specific storage bag or the second unit 200 to prevent mixing or remaining of various materials in the main transport line 310. . Importantly, after transferring the aqueous solution containing the regenerated cells from the first unit 100 to the second unit 200, the aqueous solution may remain in the main transfer line 310, the air inlet line 330 By operating the pump 900 (the pump close to the second unit), which will be described later, in an air-introducing state, all of the aqueous solution remaining in the main transfer line 310 may be transferred to the second unit 200. If only the pump 900 is operated without introducing air, a negative pressure is formed in the main transfer line 310, so that it is difficult to transfer the remaining aqueous solution by the pump 900. Inflow is essential.

The measuring line 340 is also branched from the main transfer line 310 and the pressure sensor 380 is installed at the end thereof. The pressure sensor 380 continuously measures the pressure applied to the main transfer line 310. In particular, when the adipose tissue flows into the first unit 100, it is necessary to accurately measure the pressure.

Meanwhile, the filter 370 is installed in the main transfer line 310. The filter 370 is to filter the large tissues, such as agglomerated collagen, from entering the second unit 200 through the main transfer line 310. The filter 370 has a multi-layered structure in which two mesh pockets overlap each other, and a small pocket is placed in a large pocket. An opening is formed at one side of the filter 370 such that a liquid or a small particle material may pass through the filter 370, but a large tissue such as collagen may be caught by the filter 370 and no longer flowable.

The filter 370 is installed immediately before the connection line 325 leading to the waste storage bag 395 on the movement path of the fluid moving from the first unit 100 to the second unit 200. Therefore, in order to transfer the tissue such as the collagen mass directly to the waste storage bag 395 without passing through the filter 370, it is branched from the main transfer line 310 and bypasses the filter 370 to the connection line 325. The pass line 350 is installed.

In addition, the auxiliary pipe 140 of the first unit 100 and the second discharge pipe 230 of the second unit 200 are provided with an exchange line 360 for exchanging air with the outside, the exchange line 360 At the end of the filter 361 is provided a filter for preventing contamination.

On the other hand, the optical sensor 317 is installed in the main transfer line 310 between the first unit 100 and the pump 900. The optical sensor 317 can detect the color of the fluid flowing through the main transfer line 310, in particular the yellow fat tissue or fat cells discharged from the first unit 100 through the entrance tube 130 When the yellow color is detected, a signal indicating that fat cells or fat tissues are being discharged is sent to a controller (not shown) to be described later.

In addition, a sensor 318 is installed between the second unit 200 and the pump 900. The sensor 318 detects the inflow and outflow of air through the first access pipe 220 and sends a signal to a controller (not shown).

In addition, in the present invention, a plurality of connection lines (321 ~ 326), air inlet line 330, bypass line 350 is provided with a valve 400 for selectively opening and closing these lines. The configuration of the valve 400 installed in all the lines is the same.

Referring to the drawings, the configuration of the valve 400 will be described.

FIG. 5 is a schematic perspective view of a pre-installation state of the valve illustrated in FIG. 1, FIG. 6 is a schematic perspective view of a state where the valve illustrated in FIG. 5 is installed, FIG. 7 is a schematic cross-sectional view taken along line aa of FIG. It is schematic sectional drawing of the bb line | wire of FIG.

5 to 8, the valve 400 includes a body 410, a push bar 420, and a cover 430.

The main body 410 is provided with a seating portion 411 on the upper surface so that a plurality of lines can be mounted. The seating portion 411 is disposed long from the rear of the body 410 toward the front. In addition, the support wall 412 is formed to protrude from one side of the seating portion 411 long along the mounting direction of the seating portion 411, and a pair of protruding wall portions 413 are formed at the other side of the seating portion 411. The mounted line is prevented from deviating to the side.

The groove 415 is formed below the seating portion 411. The groove part 415 is formed in the direction which intersects the arrangement direction (same as the arrangement direction of a line) of the mounting part 411. In particular, in the present embodiment, the groove portion 415 is disposed orthogonal to the seating portion 411. That is, the groove portion 415 extends between the pair of protruding wall portions 413 from the seating portion 411.

As shown in FIG. 7, the groove portion 415 has a cross-sectional shape of the letter 'v'. When the push bar 420 to be described later is pushed down to press the line, as shown in the enlarged view of FIG. 7, both side edges of the push bar 420 are two points p of the lines 321 to 326 and 330. Are pressed to the inclined surface of each of the 'v' shaped grooves to close the line. Since the push bar 420 presses two points in the line, the closing of the line is ensured. However, both sides of the push bar 420 is rounded to prevent damage to the line when pressing the line.

The push bar 420 is for opening or closing the connection line 321 to 326, the air inflow line 330 or the bypass line 350, and the push bar 420 is coupled to the main body 410 to be elevated. do. When the push bar 420 descends and presses the lines, the lines are closed, and when raised, the pressure is released and the lines having the elastic restoring force as ductile are restored to their original state and the lines are opened.

The push bar 420 includes a pressing part 421 disposed horizontally and a connecting part 422 extending vertically downward from the pressing part 421. The connection part 422 is connected to the driving means for elevating the push bar 420. As the driving means, various configurations, such as a cylinder and a motor, may be employed. In this embodiment, a solenoid (not shown) is employed. That is, the lower side of the connecting portion 422 is connected to the solenoid using the electromagnetic and the push bar 420 is elevated according to the application of the power. In addition, the push bar 420 is limited to the rising displacement due to the locking step 417 of the main body 410.

The cover 430 covers the upper portion of the main body 410 to prevent the lines from escaping upward. In the present embodiment, the cover 430 is hingedly coupled to the upper portion of the main body 410. The cover 430 is formed with a slot 431 long to allow the push bar 420 to pass therethrough.

As the valve 400 having the above configuration is elevated by driving the solenoid, the connection line, the air inflow line, or the bypass line are selectively opened and closed.

On the other hand, the pump 900 provides a driving force to move the material in the transfer unit 300, including the main transfer line (310). In this embodiment, the pump 900 is a peristaltic pump (peristaltic pump) is used, each one is installed on each side of the main transfer line (310). Both sides of the main transfer line 310 means a position close to the first unit 100 and the second unit 200 after all.

The peristaltic pump 900 is for pressurizing fluid flowing in an elastically compressible and resilient tube. The lines such as the main transfer line, the connection line, etc. are all made of a tube that can be elastically compressed and restored.

The specific configuration of the peristaltic pump 900 is shown in FIGS. 9 to 14.

FIG. 9 is a schematic perspective view of the peristaltic pump illustrated in FIG. 1, FIG. 10 is a schematic perspective view of an open state of the cover of the peristectic pump illustrated in FIG. 9, and FIG. 11 is a schematic cross-sectional view taken along line cc of FIG. 9. to be.

Referring to the drawings, the peristaltic pump 900 includes a base member 910, a rotating unit 920, a moving member 930, a pressing member 940, and a spring 950.

The base member 910 serves as a pedestal for mounting the rotating unit 920, the moving member 930, and the like, and in this embodiment, the base member 910 has a flat plate shape. On one side of the base member 910 is provided a protective wall 911 protruding upward to protect the rotating unit 920 to be described later, the line insertion groove into which the main transfer line 310 is fitted on both sides of the protective wall 911. 912 is formed. In addition, a plurality of screw holes (not shown) to which screws can be fastened are formed at both side surfaces of the base member 910.

An accommodating part 913 is formed concavely on the base member 910, and the accommodating part 913 is provided with a guide rail 914. The guide rail 914 is disposed long along the longitudinal direction of the base member 910. And the guide rail 914 is provided with a linear movable body 915 slidable along the guide rail 914.

13 and 14, the rotating unit 920 is for pumping by pressing the main transfer line 310 together with the pressure member 940 to be described later, the rotating plate 921 and the pressure roller 922, the motor (Not shown) and guide roller 923 are provided.

The rotating plate 921 is horizontally disposed and includes an upper plate 921a and a lower plate 921b spaced apart from each other, and a rotating shaft 921c is formed to connect the upper plate 921a and the lower plate 921b to each other. The rotating shaft 921c penetrates through the base member 910 and is connected to a motor (not shown) disposed below the base member 910. The rotating shaft 921c is rotatably supported by the base member 910 by the bearing b. In this embodiment, since the motor is capable of forward and reverse rotation, the upper plate 921a, the lower plate 921b, and the rotation shaft 921c may be rotated together in the forward or reverse direction as the motor is operated.

The pressure roller 922 is for pressurizing the main feed line 310, and a plurality of pressure rollers 922 are installed between the upper plate 921a and the lower plate 921b. In this embodiment, the pressing rollers 922 are disposed at three angular intervals of 120 ° along the circumferential direction of the rotating plate 921 around the rotating shaft 921c. And, as shown in Figure 14, the pressure roller 922, the outer peripheral surface is disposed so as to slightly project out of the rotating plate 921.

The guide roller 923 is provided between the pressure rollers 922 to guide the main feed line 310 so as not to be separated between the upper plate and the lower plate. The guide roller 923 is formed in a concave center portion, and as shown in FIGS. 13 and 14, the main feed line 310 is inserted into the concave groove portion 923a and the pressure roller 922 and the guide roller. Wound at 923.

In addition, since the pressing roller 922 and the guide roller 923 are surface friction with the main transfer line 310 according to the rotation of the rotating plate 921, the main transfer line 310 and the rollers may be worn. In this embodiment, the pressure roller 922 and the guide roller 923 are preferably rotatably installed to relieve surface friction. In this embodiment, the pressure roller 922 and the guide roller 923 are rotatable with respect to the central axis c.

The moving member 930 is installed on the upper portion of the base member 910 so as to reciprocate in a direction in which the rotating unit 920 approaches and is spaced apart from each other. In this embodiment, the movable member 930 is coupled to the linear movable body 915 installed in the base member 910 and is movable along the guide rail 914.

In addition, the moving member 930 is formed with a through hole 938 penetrating between the front and rear of the moving member 930 along the direction in which the guide rail 914 is disposed. One through hole 938 is provided on each side of the moving member 930.

And both sides of the moving member 930 is formed with wings 935 protruding to the outside of the base member 910. Referring to Figure 15, the wing portion 935 is formed with a threaded hole formed on the inner peripheral surface.

A guide rail 931 is also installed on the upper surface of the moving member 930. This guide rail 931 is also disposed in the same direction as the guide rail 914 provided on the base member 910. Similarly, the linear guide body 931 slidable along the guide rail 931 is also provided in the guide rail 931 on the upper surface of the movable member 930.

The pressing member 940 is for pressing the main transfer line 310 toward the pressing roller 922 of the rotating unit 920. The pressing member 940 is installed to be relatively movable with respect to the moving member 930. More specifically, in this embodiment, the pressing member 940 is fixed to the linear moving body 932 by a bolt 933. That is, the pressing member 940 is slidably installed on the guide rail 931.

A skirt portion 942 is vertically formed on the rear portion of the pressing member 940 to prevent the movable member 930 from being exposed to the outside, and through holes formed in the movable member 930 on both sides of the skirt portion 940. A hole 943 is formed in communication with 938. In addition, a pressing surface 944 in contact with the main transfer line 310 is formed on the front surface of the pressing member 940. The pressing surface 944 is formed in an arc shape along the shape of the rotating unit 920 so that the main transfer line 310 can be in close contact with the rotating unit 920.

The spring 950 is for elastically pressing the pressing member 940 toward the rotation unit 920. To this end, the spring 950 is supported between the front surface of the moving member 930 and the support surface 941 of the pressing member 940.

In order to prevent the spring 950 from being separated between the movable member 930 and the pressing member 940, the screw s is used in the present embodiment. The screw s is fastened to the support surface 941 of the pressing member 940 through the through hole 938 of the moving member 930. The screw s is not coupled to the moving member 930 and passes only through the through hole 938. The head of the screw s does not pass through the through hole 938 and is caught by the rear surface of the moving member 930. . Since the spring 950 is fitted to the screw (s), it is possible to stably elastically press the pressing member 940.

Meanwhile, when the moving member 930 is moved toward the rotating unit 920, the pressing member 940 is also moved so that the pressing member 940 contacts and presses the rotating unit 920, and the spring 950 is compressed. When releasing the force pushing the pressure member 940 in the state that the moving member 930 is not fixed, the spring 950 pushes the moving member 930 in the opposite direction of the rotating unit 920, so that the pressing member ( 940 is unable to pressurize the main transfer line 310.

That is, in order for the pressing member 940 to pressurize the main feed line 310 continuously, the pressing member 940 has to be fixed so that the moving member 930 is not moved by an external force after moving to a predetermined point.

In this embodiment, the fixing member 950 and the stopper 964 is provided for this purpose.

The fixing member 950 functions to restrain the moving member 930 from moving. In this embodiment, a plurality of screw holes (not shown) are formed at both sides of the base member 910, and the fixing member 9 is coupled to the base member 910 by screws 954. In more detail, the fixing member 950 is formed long along the moving direction of the moving member 930, and the fixing member 950 has a long hole 952 penetrating between one side and the other side thereof. The long hole 952 is disposed long in the fixing member 950 along the moving direction of the moving member 930. The screw 954 is fastened to the screw hole of the base member 910 through the long hole 952. Since the fixing member 950 is loosely fastened in the state where the screw 954 is fastened, the position of the fixing member 950 may be adjusted because the fixing member 950 is movable along the long hole 952.

The upper portion of the fixing member 950 has an insertion groove formed in a direction crossing the moving direction of the moving member 930. In this embodiment, the insertion groove portion 951 is formed along the vertical direction. The insertion groove portion 951 is for preventing the positional movement of the moving member 930 by inserting the stopper 964 to be described later. In addition, the position of the insertion groove 951 may be changed as the position of the fixing member 950 moves.

Meanwhile, in the present embodiment, the fixing member 950 is separately provided and coupled to the base member 910, but according to the embodiment, the insertion groove may be formed directly on the base member 910.

The stopper member 960 includes a stopper 964, a hollow tube 961, and a handle 961. The stopper 964 is installed in the moving member 930 and inserted into the insertion groove 951 to limit the movement of the moving member 930. In this embodiment, the stoppers 964 are provided at both sides of the base member 910, respectively, and are inserted into the hollow tube 961. The upper ends of the stoppers 964 disposed at both sides of the base member 910 are respectively fixed to the handles 967. Since the hollow tube 961 is not coupled to the handle 967, the handle 967 and the stopper 964 may be moved relative to the hollow tube 961 along the up and down direction.

A hollow fastening bolt 962 is coupled to the lower portion of the hollow tube 961. A screw portion 962a is formed on the lower outer circumferential surface of the fastening bolt 962 and is fastened to a screw hole in which a wing portion of the moving member 930 is formed. As the fastening bolt 962 is screwed to the moving member 930 as described above, the entire stopper member 960 is coupled to the moving member 930. The lower end of the stopper 964 is disposed to protrude downward from the hollow tube 961 and the fastening bolt 962.

The spring 963 is fitted to the stopper 964 in the fastening bolt 962. A flange portion 964a is formed at the bottom of the stopper 964, and the spring 963 is supported between the upper surface of the flange portion 964a and the fastening bolt 962 to insert the stopper 964 into the insertion groove portion 951. Elastically press toward.

In a state where the fastening bolt 962 is fastened to the moving member 930 and fixed, when the handle 967 is pulled upward, the stopper 964 is also pulled together to be separated from the insertion groove 951, at which time the flange 964a is pulled out. The spring 963 is kept compressed. On the contrary, when the force that pulls the handle 967 is released, the compressed spring 963 elastically restores the pressure and presses the flange portion 964a to press the stopper 964 into the insertion groove portion 951. That is, the stopper 964 is elastically biased toward the insertion groove portion 951 by the spring 963.

As described above, when the stopper 964 is inserted into the insertion groove 951, the position of the moving member 930 is fixed. Since the position of the movable member 930 is fixed, the spring 950 installed between the pressing member 940 and the moving member 930 presses the pressing member 940 toward the rotating unit 920.

When the position of the fixing member 950 is moved, the position of the insertion groove 951 is also moved, and thus the degree of compression of the spring 950 for elastically pressing the pressing member 940 can be adjusted.

The peristaltic pump 900 supports the main feed line 310 by elastically pressing the pressing member 940 toward the rotary unit 920 by a spring 950, so that the pressing force of the pressing roller 922 is the main feed. To be delivered without loss to line 310.

In addition, in the present invention, the main transfer line 310 may be used in a variety of thickness, the existing pumps could be used only in the line of fixed specifications. That is, a thick line could not be used because it could not be inserted between the roller and the support surface, and when the thickness was thin, there was a problem that the efficiency was low because the loss was high when the roller was pressed.

However, the peristaltic pump 900 employed in the present invention can be moved to the position of the pressing member 940 can be applied to all of the line of various specifications, as well as to accurately support the line using the spring 950 There is an advantage that the force of the pressure roller can be accurately transmitted to the line without loss.

Hereinafter, the regenerated cell extraction method using the regenerated cell extraction system 1000 having the above configuration will be described.

First, the adipose tissue to be separated is introduced into the first unit 100 through the entrance tube 130. For example, after pre-extracting adipose tissue from the human body through a syringe, the adipose tissue may be introduced into the separation unit 100 again. However, in order to prevent contamination during the transfer process, the syringe needle is directly plugged into the human body and the tube connected to the syringe syringe is connected to the entrance tube 130, and the inside of the sub container 110 using the pump 900. When formed in a negative pressure atmosphere can be introduced into the sub-container 110 directly from the adipose tissue human body.

As described above, when the adipose tissue is introduced into the sub-container 110, the other valves are all locked to open the valve 400 installed in the connection line 323 and operate the pump 900 to operate the washing liquid in the first unit ( Transfer to 100). Then, the rotation jig for the first unit 100 is repeated by operating the rotary jig not shown. When the rotation and stop are repeated at regular intervals, the adipose tissue and the washing solution are separated by mutual stirring, and the adipose tissue introduced with the blood-derived contaminants is washed.

When the sub-container 110 is stopped for about 1 minute, heavy components such as washing liquid, blood, and body fluid are disposed in the lower part of the sub-container 100, and the washed adipose tissue is disposed on these upper parts, and oil The component is placed on the top layer.

The pump 900 is operated again to discharge the blood, the washing liquid, and the like in the lower portion of the sub container 110 through the entrance tube 130. At this time, if the bypass line 350 is operated in an open state, wastes such as blood and washing liquid are bypassed without passing through the filter 370 and moved to the waste storage bag 395.

When all the washing liquid is discharged, the yellow tissue is discharged through the entrance tube 130. The optical sensor 317 installed in the main transfer line 310 detects the color of the fatty tissue and sends a signal to the controller. Send it out. In the controller, the operation of the pump 900 is stopped, and the above-described process is repeated three to four times to allow the adipose tissue to be completely washed.

As described above, the blood contaminants, body fluids and oil components are separated from the adipose tissue extracted from the human body, and the adipose tissue is thoroughly washed, and if necessary, the washed adipose tissue is stored in a portion of the adipose tissue storage bag ( 392) for storage. At this time, while all other valves are locked, only the valve installed in connection line 322 is opened and transferred.

Thereafter, while operating the pump 900, the collagenase is transferred from the enzyme storage bag 393 to the first unit 100 through the entry pipe 130 while only the valve 400 installed at the connection line 321 is opened. do. And when it is necessary to adjust the concentration of enzyme, some washing solution may be added.

When the collagenase enters the sub container 110, the pump is stopped and the sub container 110 is rotated and stirred for about 30 minutes. At this time, the temperature of the sub-container 110 is maintained at about 37 ° C. through the temperature adjusting means provided in the rotary jig. Collagenase breaks down adipose tissue, and the degraded components are centrifuged by the rotation of the sub vessel 110. When the rotation of the sub-container 110 is stopped and allowed to stand for about 1 minute, the aqueous solution containing the adipose derived stem cells is disposed under the sub-container 110 because it is a relatively heavy component, and the mature fat cells are formed thereon. And oil components are placed.

In the state that all the valves are locked, two pumps 900 adjacent to each of the first unit and the second unit are operated to transfer the aqueous solution containing stem cells to the second unit 200 through the entrance tube 130. do. At this time, since the aqueous solution passes through the filter 370, large tissues such as collagen are filtered out by the filter.

Even after the transfer is completed, some aqueous solution containing stem cells may remain in the main transfer line 310. In order to transfer this aqueous solution to the second unit 200 without loss, when the pump 900 adjacent to the second unit 200 is operated while only the air inlet line 330 connected to the air filter 393 is opened, As the air flows into the main transfer line 310, all of the remaining aqueous solution may be transferred to the second unit 200.

When yellow is detected by the light sensor 317 again, the aqueous solution containing the stem cells is discharged after all of the aqueous cells are discharged, thereby stopping the operation of the pumps (when transferring all the aqueous solutions at once). And, if necessary, mature fat cells and oil can be stored in separate storage bags.

As described above, when all of the aqueous solution including the stem cells is introduced into the main container 210 of the second unit 200, a process of extracting stem cells is performed. In this embodiment, the vowel member 270 and the guide member ( Only the case where 280) is not used will be described.

First, all the valves are closed and the main vessel 210 is rotated for about 5 minutes while the pumps are also stopped, and then naturally decelerated. The aqueous solution containing the stem cells is centrifuged in this process, the relatively heavy stem cells are pressed by the centrifugal force and attached to the inner wall of the receiving protrusion 217, and body fluids, washing liquids, enzymes, etc. are disposed at the bottom of the main container 110. Are accepted. An important point is to stop the rotation of the main vessel 110, not to naturally decelerate, but to reduce the natural. This is because stem cells attached to the inner wall of the receiving protrusion 217 may fall below the main container 110 by artificially applying a brake to rotation.

When the centrifugation is completed, only the connection line 325 connected to the waste storage bag 395 is opened to transfer an aqueous solution such as a body fluid to the waste storage bag 395 through the first discharge pipe 220. When air is sensed by the sensor 318 installed in the main feed line 310, since all of the aqueous solution is discharged, the operation of the pump 900 is stopped.

Then, the steps from the step of transferring the aqueous solution including the stem cells from the first unit 100 to the second unit 200 are repeated several times. That is, when the amount separated from the first unit is large, instead of moving all the aqueous solutions to the second unit at once, the stem cells may be extracted by transferring the aqueous solution containing the stem cells in several times. When the yellow adipose tissue is detected by the light sensor 317, the repeating process is terminated.

As described above, after the centrifugation of the aqueous solution including the stem cells is completed, to wash the stem cells attached to the inner wall of the protrusion accommodating portion 217, a small amount of the washing liquid is introduced into the main container 210. Then, the air is introduced again while only the air inlet line 330 is opened to introduce all the fluids in the main transfer line 310 into the second unit 200.

In a state in which all the lines are closed, the rotation and stop about the main container is repeated about five times, and then centrifuged again to naturally decelerate the main container 210. When the main container is accelerated and decelerated while rotating and stopping in this way, the stem cells attached to the inner wall of the protrusion accommodating part 217 fall to the lower part of the main container 210. And by repeating the above-mentioned stem cell extraction process several times, it is possible to ensure that foreign substances are minimally included in the finally obtained stem cells.

Finally, the stem cell extraction process is completed by only opening the connection line 326 and transferring the stem cells existing in the lower portion of the main container 210 to the stem cell storage bag 396 through the first discharge pipe 220.

As described above, the regenerated cell extraction method according to the present invention can be extracted only without the problem of contamination since only the air purified from the air filter is introduced, it is made in a completely sealed state.

First of all, in the present invention, when a specific material is exchanged with a storage bag, only a connection line connected to the storage bag needs to be opened, so the operation is very simple. In the existing system, it is not easy to operate because several valves must be opened and closed together, but the process according to the present invention can be performed very simply.

In addition, in the present method for extracting regenerated cells, an aqueous solution including a stem material, particularly stem cells, may remain in the main transfer line, thereby lowering the yield. Thus, the aqueous solution containing stem cells may be introduced without introducing a loss of air through an air inlet line. There is an advantage that it can be transferred to the second unit. This is not limited to aqueous solutions containing stem cells, but can be applied to all target materials. That is, after the target material is exchanged (transferred) through the main transfer line, if the target material remains in the main transfer line, the target material may be completely transferred to the desired position by introducing air through the air inlet line. .

As described above, in the detailed description of the present invention has been described with respect to preferred embodiments of the present invention, those skilled in the art to which the present invention pertains various modifications can be made without departing from the scope of the present invention Of course.

Therefore, the scope of the present invention should not be limited to the described embodiments, but should be determined not only by the claims below, but also by the equivalents of the claims.

Claims

A base member;

A rotating unit having a rotating plate rotatably installed at one side of the base member, a plurality of pressure rollers installed on the rotating plate to be spaced apart from each other along the circumferential direction of the rotating plate, and a motor for rotating the rotating plate;

A moving member installed on the base member so as to reciprocate in a direction approaching and spaced apart from the rotating unit, and fixed at a predetermined point;

The moving member is installed in the movable member so as to reciprocate in a direction approaching and spaced apart from the rotating unit, and an arc-shaped pressing surface is formed at one end thereof to press the elastically compressible and recoverable tube wound on the rotating unit. A pressing member pressurizing toward the roller; And

And a spring supported between the moving member and the pressing member to elastically press the pressing member toward the rotating unit.
The method of claim 1,

The moving member is formed with a through hole penetrating between the front and rear in the reciprocating direction of the moving member,

A thread is formed on the outer circumferential surface, and further includes a screw inserted into the through hole of the moving member and screwed to the screw hole formed in the pressing member.

And the spring is fitted to the screw.
The method of claim 1,

The spring is provided with two, the peristaltic pump, characterized in that each installed on both sides of the front of the moving member.
The method of claim 1,

Insertion grooves are formed on at least one of both sides of the base member,

Further provided with a stopper which is installed in the movable member so as to reciprocate in the direction in which the insertion groove is formed, which is elastically biased in the direction to be inserted into the insertion groove,

And the moving member is fixed in position when the stopper is inserted into the insertion groove.
The method of claim 1,

A fixing member coupled to at least one of both sides of the base member so as to be movable in a reciprocating direction of the pressing member, and having an insertion groove formed in a direction crossing the reciprocating direction of the pressing member;

Further provided with a stopper which is installed in the movable member so as to reciprocate in the direction in which the insertion groove is formed, which is elastically biased in the direction to be inserted into the insertion groove,

And the moving member is fixed in position when the stopper is inserted into the insertion groove.
The method of claim 5,

A screw hole is formed on the side of the base member,

The fixing member penetrates between one side and the other side and is formed with a long hole along the moving direction of the fixing member,

Peristatic pump, characterized in that the fixing member is fixed by the screw is fastened to the screw hole through the long hole.
The method of claim 1,

Peristaltic pump is installed between the pressure roller of the rotary unit, the central portion is formed concave, characterized in that it further comprises a guide roller that can be inserted into the tube.
A tissue for separating tissue, which is rotated by receiving rotational force from the outside and is inserted into the sub-container so that one end thereof is disposed below the sub-container, and has a space portion for receiving and separating the tissue. A first unit having a hollow entrance tube;

A main container for extracting regenerated cells from the separation object transferred from the first unit, the main container is rotated by receiving a rotational force from the outside and the receiving portion is formed to accommodate the separation object, and one end is disposed below the main container A second unit having a hollow first discharge pipe inserted into the main container so that the other end thereof is disposed outside the main container;

A transfer unit for exchanging fluid between the first unit, the second unit, and the plurality of reservoir bags, the transfer unit comprising a plurality of tubes elastically compressible and recoverable; And

Regenerated cells comprising a configuration of any one of the claims 1 to 7, wherein the plurality of peristaltic pump is installed in the tube of the delivery unit to pressurize and transport the fluid inside the tube; Extraction system.
The method of claim 8,

The delivery unit,

A main feed line connecting the access pipe of the first unit and the first discharge pipe of the second unit, a plurality of connection lines branched from the main feed line and each of which has a storage bag connected to an end thereof, and the first unit; And an air inlet line branched from the main transfer line between the plurality of connection lines and equipped with an air filter at an end thereof to filter external air, and a plurality of valves respectively installed at the connection line and the air inlet line. Regenerating cell extraction system, characterized in that.
The method of claim 9,

Waste storage bag is installed at the end of any one of the connection line,

A filter installed in the main transport line to filter out agglomerates of a predetermined size or more in front of a connection line connected to the waste storage bag on the transport path of the fluid moved from the first unit to the second unit;

And a bypass line branched from the main transfer line to the connecting line in which the waste storage bag is installed so that the fluid discharged from the first unit bypasses the filter.
The method of claim 9,

The air inlet line is a regenerated cell extraction system, characterized in that branching from the main transfer line prior to the connecting line on the transfer path of the fluid moved from the first unit to the second unit.