WO2013051816A2 - Cell treatment application system for dealing with skin defects, and cell harvesting unit, pulverizing unit, and atomizing unit for same - Google Patents

Abstract

Disclosed are a cell treatment application system for dealing with skin defects such as burns, wounds, and leukoplakia in real time by using cells or human tissue, and to a cell harvesting unit, pulverizing unit, and atomizing unit for same. The disclosed cell treatment application system for dealing with skin defects comprises: a main body; a pulverizing unit provided in the main body for pulverizing predetermined tissue housed and provided in a pulverizing tube together with pulverizing beads; a harvesting unit for acquiring skin cell groups from the tissue fragments pulverized in the pulverizing unit; and an atomizing unit provided in the main body for atomizing the skin cell groups acquired by the harvesting unit onto a portion for cell treatment.

Description

Cell treatment application system for the treatment of skin defects and cell harvesting unit, grinding unit and spray unit therefor

The present invention relates to a cell treatment application system for the treatment of skin defects such as burns, wounds, vitiligo and the like using a cell or human tissue in real time, and a cell obtaining unit, a grinding unit, and a spray unit therefor.

If a defect occurs in the skin tissue such as burns, wounds, or depigmented skin, a scar or pigment abnormality may be left after surgery or treatment.

In general, when skin defects such as burns, wounds and vitiligo occur, the skin is treated with gauze moistened with petrolatum-based ointment, and antibiotics such as penicillin and sulfamine are added to prevent secondary infection. In addition, a separate analgesic was administered to alleviate the pain.

On the other hand, such a treatment method requires a long-term treatment for a short period of time from 2 to 3 weeks to a few months, and the cost is considerable. There are disadvantages.

As another method of conventional skin defect treatment, a method of using a cell therapy using autologous cells in case of burn is known. However, the conventional method for preparing a cell therapeutic agent using autologous cells uses a method of harvesting specific cells through enzyme treatment and culturing the collected cells for several days. Accordingly, there is a problem that the conventional cell therapy agent is not applicable to a patient who takes a long time to incubate a cell and has a severe and urgent treatment of a skin defect such as a burn.

The present invention has been made in view of the shortcomings of the conventional skin defect treatment method as described above, it is possible to spray the target skin from the tissue or human tissue by treating the target tissue to be applied to the obtained skin defect treatment in real time. It is an object of the present invention to provide a cell treatment application system for the treatment of skin defects of a structure, and a cell obtaining unit, a grinding unit, and a spray unit for the same.

In order to achieve the above object, the cell treatment application system for the treatment of skin defects according to the present invention, the body; A pulverizing unit provided in the main body and pulverizing a predetermined target tissue accommodated in a pulverization tube together with a pulverizing bead; A obtaining unit for obtaining a skin cell group from tissue crushed in the grinding unit; It is provided in the main body, and comprises a spray unit for spraying the skin cell group obtained in the obtaining unit to the cell treatment target site.

The grinding unit, the housing is installed in the main body; A cover member covering the housing; A grinding drive source installed in the housing and providing rotational force; A holder installed on the rotating shaft of the grinding drive source and mounted with the grinding tube; A striking part installed in the housing to be operable between a striking position striking the milling tube and a spaced position spaced apart from the milling tube when the holder is rotated; It may include a mode switching unit for switching the mode so that the striking portion is variable in position between the striking position and the spaced position.

The striking unit and the striking body; And at least one striking member rotatably installed with respect to the striking main body, wherein the mode switching unit is installed to be capable of relatively reciprocating rotational movement with respect to the housing, and has a mode switching hole in which a portion of the striking member is inserted therethrough. A conversion body; It may include an operation unit for reciprocating the mode switching body.

The striking unit and the striking body; At least one striking member rotatably installed relative to the striking body, wherein the mode switching unit is installed on the housing and provides a driving force; It may be installed to reciprocate relative to the cylinder body, it may include an operation unit for adjusting the striking member rotatably with respect to the striking body.

The striking unit and the striking body; And at least one striking member rotatably installed with respect to the striking body, wherein the mode switching unit is slidably installed on the striking body, and a slider for rotatably adjusting the striking member relative to the striking body; ; It may include a driving unit for providing a driving force to the slider.

The drive unit, the slide driving source is installed on the hit body; It may include a power transmission unit for converting the rotational movement provided from the slide driving source to a linear reciprocating motion provided to the slider.

The grinding unit may further include a clamp for clamping the grinding tube mounted to the holder.

Here, the clamp includes a clamp body for preventing the grinding tube mounted in the grinding tube mounting groove of the holder from being separated during operation; It may include a fixing cap for fixing the clamp body to the rotating shaft of the grinding drive source.

The clamp may include a guide shaft fixedly installed inside the cover member; A clamp main body rotatably mounted on the guide shaft and supporting the grinding tube mounted on the grinding tube mounting groove of the holder such that the grinding tube is not separated during operation; An elastic member interposed between the cover member and the clamp body to elastically bias the clamp body in a direction in which the grinding tube is pressed; It may include a rotation support for supporting the clamp body freely rotatable with respect to the cover member.

The obtaining unit includes a filter unit for filtering the skin cell group from the tissue crushed material contained in the grinding tube; It may include a spray tube for receiving the skin cell group filtered by the filter unit.

The filter unit, the coupling member is lockably coupled to the grinding tube; It may include a filter member installed in the opening of the grinding tube by the coupling member.

The apparatus may further include a locking member provided in a shape corresponding to each of the opposite surfaces of the grinding tube and the coupling member, wherein the filter unit filters the skin cell group to separate the grinding tube and the spray tube. The coupling member and the filter member may be separated from the spray tube and positioned at the crushing tube side.

The spray tube may further include a first protective cap covering the filter unit and protecting the spray tube.

The filter unit, the coupling member coupled to the grinding tube; A filter member installed in the opening of the grinding tube by the coupling member; Covering the coupling member and the filter member, it may include a first protective cap detachable from the coupling member by an external force.

In addition, a plurality of bridges connecting the first protective cap and the coupling member and cut by an external force may be provided between the second protective cap and the coupling member.

The spray tube, the spray tube body having an inner receiving space for accommodating the skin cell group and the spray hole for spraying the skin cell group, and coupled to the coupling member; A second protective cap detachably installed on the spray tube body to cover the spray hole; It may include a piston that is provided to reciprocate in the receiving space of the spray tube body.

The spray tube may further include a gasket installed in the spray hole.

The spraying unit includes: a spray gun for accommodating the spraying tube having the second protective cap installed in the spraying tube body and pressurizing the piston unit to spray a skin cell group accommodated in the spraying tube; Being installed in the spray gun, it may include a nozzle for spraying the skin cell group injected from the spray tube to the affected part.

The spray gun includes a trigger; A loading unit for loading the spray tube; In conjunction with the operation of the trigger, it may include a pressing unit for pressing the piston unit so that the skin cell group accommodated in the spray tube.

The spray gun may supply power to the pressurizing unit, and may further include a power supply unit embedded in the spray gun.

The nozzle includes a nozzle body having an injection pipe passing through the second protective cap and coupled to the injection hole of the spray tube; A spray unit for spraying the skin cell group supplied from the spray tube; It may include a control unit for adjusting the injection amount from the spraying unit.

In addition, the cell treatment application system according to the present invention may further include a test unit provided in the main body, the test unit for testing the skin cell group obtained from the tissue crushed in the grinding unit.

The inspection unit includes a transfer unit for transferring the inspection object; A microscope for enlarging the tissue to be transferred; It may be installed in the main body, and may include a display unit for displaying the enlarged tissue of the inspection object.

The transfer unit, and the stage is installed to be horizontally movable with respect to the inspection unit mounting portion provided in the main body; A lifting part provided to be movable up and down by a predetermined driving source in a direction perpendicular to the stage; And provided on the lifting unit, the upper surface may include a stage for mounting the object to be inspected.

The skin cell group may include at least one of keratinocytes, melanocytes and fibroblasts.

Cell treatment application system for the treatment of skin defects according to the present invention and a cell harvesting unit, a grinding unit and a spray unit for the treatment of cells in real time using cells or human tissue without the step of separating and culturing the cells by enzyme treatment By separating and purifying and applying to the affected area, there is an advantage that the cell treatment and application can be easily and conveniently performed in a short time.

In addition, the cell treatment application system for the treatment of skin defects according to the present invention by adjusting the striking portion hitting the crushing tube containing the target tissue between the striking position and the spaced apart position, separating the cells by the impact method There is an advantage in that it can perform both a homogenizer and a centrifuge function to separate cells by centrifugation. Accordingly, the application range of the cell treatment application system can be widely applied not only to burn treatment but also to extracting stem cells from adipocytes.

In addition, the cell treatment application system for the treatment of skin defects according to the present invention, the skin cell group is obtained in a series of processes of acquiring the skin cell group from the tissue debris contained in the grinding tube in the state of being pulverized in the grinding unit and mounting it to the spray unit. By not exposing, the obtained skin cell population can be prevented from being contaminated by the external environment.

1 and 2 are schematic perspective views of a cell treatment application system according to two embodiments of the present invention.

Figure 3 is a schematic cross-sectional view showing a grinding unit for a cell treatment application system according to the first embodiment.

Figure 4 is an exploded perspective view showing the main portion of the grinding unit separated according to the first embodiment.

5 is a perspective view showing a mode switching unit of the grinding unit according to the first embodiment.

6 and 7 are views showing the relationship between the striking unit and the mode switching unit at each of the separation position and the striking position of the grinding unit according to the first embodiment.

8 is a schematic cross-sectional view showing a grinding unit according to a second embodiment.

9 is a schematic cross-sectional view showing a grinding unit according to a third embodiment.

10 is a schematic plan view of the grinding unit of FIG.

Figure 11 is a schematic perspective view showing the main portion of the grinding unit according to the third embodiment.

12 and 13 are views showing the placement relationship between the striking unit and the mode switching unit in each of the separation position and the striking position of the grinding unit according to the third embodiment.

Figure 14 is a schematic perspective view showing the main portion of the grinding unit according to the fourth embodiment.

15 and 16 are views showing the relationship between the striking unit and the mode switching unit in each of the separation position and the striking position of the grinding unit according to the fourth embodiment.

Figure 17 is a schematic cross-sectional view showing an inspection unit according to the present invention.

18 is a schematic perspective view showing a microscope of the inspection unit according to the present invention.

19 is a schematic cross-sectional view showing a grinding tube after the grinding process according to the present invention.

20 is a schematic cross-sectional view showing the obtaining unit according to the first embodiment.

21 is an exploded perspective view showing a coupling relationship between the obtained unit and the grinding tube according to the first embodiment.

22 to 24 are each a view for explaining the coupling and separation process of the obtaining unit and the grinding tube according to the first embodiment.

25 is a schematic view showing a state in which the obtaining unit and the nozzle are combined according to the first embodiment.

FIG. 26 is a schematic view for explaining spray operation in a state in which a obtaining unit and a nozzle are coupled according to the first embodiment; FIG.

Figure 27 is a schematic exploded perspective view showing the main portion of the obtaining unit according to the second embodiment.

28 is a schematic cross-sectional view showing the filter unit of the obtaining unit according to the second embodiment coupled to the grinding tube.

29 is a schematic view showing a spray unit according to an embodiment of the present invention.

30 to 32 are views for explaining the operating state of the nozzle of the spray unit according to an embodiment of the present invention.

Hereinafter, with reference to the accompanying drawings will be described in detail the cell treatment application system and a cell obtaining unit, a grinding unit and a spray unit for the same in the embodiment of the present invention.

1 and 2 are schematic perspective views of a cell treatment application system according to two embodiments of the present invention.

Referring to the drawings, the system 1 according to the embodiment of the present invention is provided with a main body 100, a main unit 100, a grinding unit 200 for grinding a predetermined target tissue 20, and a grinding unit 200 Spray unit 400 for spraying the skin cell group (25) obtained in the skin cell group (25 of FIG. 19) and the skin cell group obtained in the obtaining unit 300 from the tissue crushed in the ).

Here, the target tissue 20 is provided accommodated in the grinding tube 10 together with the grinding beads 30, the skin cell group 25 obtained from the target tissue 20 is keratinocytes, melanocytes And fibrous cells.

In addition, the system 1 according to the present invention may further include a test unit 500 provided in the main body 100 and inspecting the skin cell group 25 obtained from the tissue crushed in the grinding unit 200. have.

In addition, the system 1 according to the present invention may further include a suction gun 600 provided on the main body 100 to suction and remove foreign substances of the predetermined target tissue 20.

The main body 100 includes a working unit 110 for performing a task of accommodating a predetermined target tissue 20 in the grinding tube 10, a grinding unit mounting unit 130 in which a grinding unit 200 is installed, and an inspection unit 500. It includes a test unit mounting unit 150 is installed, and a spray unit mounting unit 170 in which the spray unit 400 is installed.

The main body 100 may be formed in a '-' shape as shown in FIG. 1 in consideration of the operator's treatment convenience and the installation space of the present system. In addition, the main body 100 may be formed in a straight shape as shown in FIG. 2 in consideration of an installation space of the present system. Here, the work unit 110 is provided on one side with the grinding unit mounting unit 130 interposed therebetween, and the test unit mounting unit 150 and the spray unit mounting unit 170 are provided on the other side. Therefore, the operator may keep the copper wire in the clockwise or counterclockwise direction in performing the series of procedures sequentially.

In addition to the grinding unit 200, the grinding unit mounting unit 130 of the main body 100 may be equipped with a obtaining unit 300. Here, the obtaining unit 300 may be mounted in the mounting space 130 provided in the grinding unit mounting unit 130 as shown in Figure 1 and 2 or may be accommodated in a container (not shown) provided separately from the main body 100. have.

The grinding unit mounting unit 130 may be equipped with an operation panel (OPE; Operational Panel Equipment, 700) for controlling the overall operation of the components including the grinding unit 200 and the inspection unit 500 of the present system.

The grinding unit 200 is provided in the grinding unit mounting unit 130 on the main body 100, and grinds a predetermined target tissue 20 accommodated and provided in the grinding tube 10 together with the grinding beads 30.

Hereinafter, various embodiments of the grinding unit 200 will be described in detail with reference to FIGS. 3 to 14.

Figure 3 is a schematic cross-sectional view showing a grinding unit according to the first embodiment, Figure 4 is a partial cross-sectional perspective view showing the main portion of the grinding unit, Figure 5 is a perspective view showing the mode switching unit of the grinding unit.

3 to 5, the grinding unit 200 according to the first embodiment is installed in the housing 210 and the housing 210 installed in the grinding unit mounting unit 130 of the main body 100 to provide rotational force. The grinding drive source 220, the holder 225, the grinding tube 10 is mounted, the impact portion 250 and the mode switching unit 260. In addition, the grinding unit 200 may further include a clamp 230 for clamping the grinding tube 10 mounted on the holder 225.

Holder 225 is installed on the rotating shaft of the grinding drive source 220, and includes a grinding tube mounting groove 225a on which the grinding tube 10 is mounted. Here, the holder 225 may include a plurality of grinding tube mounting groove so that the plurality of grinding tubes 10 can be mounted at the same time.

The clamp 230 has a clamp main body 231 for preventing the grinding tube 10 mounted on the grinding tube mounting groove 225a of the holder 225 from being separated during operation, and the clamp main body 231 for the grinding driving source 220. Fixing cap 235 is fixed to the rotation axis of the. Therefore, when the grinding drive source 220 is driven in rotation, the grinding tube 10 mounted on the holder 225 is driven to rotate in a fixed state so as not to be separated by the clamp 230.

The striking part 250 is operable between a striking position (see FIG. 7) hitting the milling tube 10 when the holder 225 rotates, and a spaced position (see FIG. 6) spaced apart from the milling tube 10. It is installed in the housing 210.

Here, as shown in Figure 7, when the crushing unit 200 operates in a state in which the hitting portion 250 is located in the hitting position, performs a homogenizer (homogenizer) function to separate the cells by the impact method do.

On the other hand, as shown in Figure 6, when the crushing unit 200 is operated in a state in which the striking portion 250 is located in the striking position 250 in the spaced apart position, the cells are separated by a centrifugation method (centrifugation) Performs a centrifuge function.

The striking unit 250 includes a striking body 251 and a striking member 255 rotatably installed with respect to the striking body 251. Here, the striking member 255 has a plurality of striking members 255 which are spaced apart from each other by a milling drive source 220 so that the holder 225 strikes the milling tube 10 a plurality of times during one rotation. It may include. In this case, each of the plurality of striking members 255 is installed with respect to one striking body 251 or independent of each of the striking part mounting grooves 213 provided in various places of the housing 210 as shown in FIG. 3. It may be provided in each of the plurality of hitting body 251 disposed in the.

The mode switching unit 260 switches the mode so that the striking unit 250 is variable in position between the striking position and the spaced position. To this end, the mode switching unit 260 may include a mode switching body 261 and an operation unit 265 for driving the mode switching body 261 to reciprocally rotate.

The mode switching body 261 is installed to reciprocally rotate relative to the housing 210. The mode switching body 261 is formed with a mode switching hole 263 through which a portion of the striking member 255 is inserted. The operation unit 265 is to manually or automatically drive the mode switching body 261 reciprocating rotation, by the operation of the operation unit 265, while the position of the mode switching hole 263 is variable, the striking member 255 is Allow for reciprocating movement between the striking and spaced positions.

5 illustrates an example of the operation unit 265 as a knob provided on the mode switching body 261 as an example. In this case, the operating part 265 is exposed while the cover member 215 covering the upper part of the housing 210 is opened, and the mode can be switched by manual operation of the operating part 265. On the other hand, the operation unit 265, including a separate drive source (not shown) provided in the grinding unit, and thereby a power transmission structure (not shown) for rotating the mode switching body 261, the operation of the operation panel 700 You can switch modes automatically.

FIG. 6 is a view illustrating an arrangement relationship between the striking unit 250 and the mode switching unit 260 in a spaced position, and FIG. 7 illustrates an arrangement relation of the striking unit 250 and the mode switching unit 260 in the striking position. The figure shown.

The rotational direction of the striking member 255 rotatably installed with respect to the striking body 251 is determined according to the rotational position of the mode switching body 261. Referring to FIG. 6, when the mode switching main body 261 is rotated in the direction of arrow A by the operation (clockwise rotation) of the operation unit 265, the striking member 255 is in the mode of the mode switching main body 261. Interfering with the first side wall 263a of the switching hole 263 is located at a spaced position.

On the other hand, when the mode switching body 261 positioned as shown in FIG. 6 is rotated in the direction of arrow B (counterclockwise), the striking member 255 is the second side wall 263b of the mode switching hole 263. Interfering with each other, the mode is switched from the spaced position to the striking position, as shown in Figure 7, the mode switching body 261 and the striking member 255 is located. When the grinding unit 200 operates in this state, the striking member 255 hits the grinding tube 10, thereby performing a homogenizer function.

8 is a schematic cross-sectional view showing a grinding unit according to a second embodiment of a cell treatment application system according to the present invention.

Referring to FIG. 8, the grinding unit 200 according to the second embodiment includes a housing 210, a grinding drive source 220, a holder 225, a striking unit 250, a mode switching unit 260, and a clamp 240. ). Compared with the grinding unit 200 according to the first embodiment, only the structure of the clamp 240 is distinguished from the changed point, the structure, function and arrangement of the other components are substantially the same. Therefore, in the present embodiment, the components except for the clamp 240 are given the same reference numerals as the components of the same member name of the grinding unit 200 according to the first embodiment, the detailed description thereof will be omitted.

The clamp 240 is mounted inside the cover member 215 to be linked to the opening and closing of the cover member 215 to enable the clamping operation. That is, the clamp 240 automatically opens when the cover member 215 is opened, and presses the grinding tube 10 when the cover member 215 is closed.

To this end, the clamp 240 includes a guide shaft 241, a clamp body 243, an elastic member 245, and a rotation support part 247 fixedly installed inside the cover member 215. The clamp body 243 is installed on the guide shaft 241 so as to be lifted and rotated, and supports the grinding tube 10 mounted in the grinding tube mounting groove 225a of the holder 225 so as not to be separated during operation. The elastic member 245 is interposed between the cover member 215 and the clamp main body 243 to elastically bias the clamp main body 243 in the direction in which the grinding tube 10 is pressed. The elastic member 245 may be composed of a compression spring inserted into the outer circumference of the guide shaft 241 as shown in FIG. In addition, the elastic member 245 is not limited to the compression spring, and can be variously modified, such as a tension spring, a separate elastic member, within the configuration range for elastically biasing the clamp body 243.

The rotation support part 247 supports the clamp body 243 to be freely rotatable with respect to the fixed cover member 215. As shown in FIG. 8, the rotation support part 247 may include a bearing mounted between the elastic member 245 and the clamp body 243 with the support 249 therebetween.

By configuring the clamp 240 in the cover member 215 as described above, the clamp body 243 for clamping the pulverization tube 10 in accordance with the opening and closing of the cover member 215 can be automatically detachable. . Therefore, unlike the grinding unit 200 according to the first embodiment, since a separate fixing cap (235 of FIG. 3) is unnecessary, there is an advantage that the clamping operation can be simplified.

Figure 9 is a schematic cross-sectional view showing a grinding unit according to a third embodiment of the cell treatment application system according to the present invention, Figure 10 is a schematic plan view of the grinding unit of Figure 9, Figure 11 is a cell treatment according to the present invention It is a schematic perspective view showing the main part of the grinding unit according to the third embodiment of the application system.

Referring to the drawings, the grinding unit 200 according to the third embodiment is the housing 211, the grinding drive 220, the holder 225, the striking portion 281, the mode switching unit 285 and the clamp 235 It includes. Compared with the grinding unit 200 according to each of the first and second embodiments, the structure of the other components, which are distinguished only by changing the configurations of the striking unit 281 and the mode switching unit 285, The functions and arrangements are substantially the same. Therefore, in the detailed description of the present embodiment, the components except for the housing 211, the hitting portion 281 and the mode switching unit 285 are the same as the components of the same member name of the grinding unit 200 according to the first embodiment. The same reference numerals have been given, and detailed description thereof will be omitted.

The housing 211 is provided with at least one striking portion 281 and the mode switching unit 285.

The striking portion 281 is operable between a striking position (see FIG. 13) that strikes the milling tube 10 when the holder 225 rotates, and a spaced position (see FIG. 13) spaced apart from the milling tube 10. It is installed in the housing 211.

The striking portion 281 includes a striking body 283 and a striking member 284 rotatably provided to the striking body 283. To this end, the striking body 283 is provided with a first pivot pin 283a coupled through the striking member 284.

Here, the striking member 284 has a plurality of striking members 284 disposed to be spaced apart from each other at predetermined intervals so that the holder 225 strikes the milling tube 10 a plurality of times when the holder 225 is rotated by the milling drive source 220. It may include. In this case, each of the plurality of striking members 284 is provided in each of the plurality of striking bodies 283 independently disposed in each of the striking part mounting grooves 211a provided at various places of the housing 211, as shown in FIG. Can be prepared.

The mode switching unit 285 switches the mode such that the striking unit 284 is variable in position between the striking position and the spaced position. To this end, the mode switching unit 285 includes a cylinder body 287 and an operation unit 289 which is reciprocally movable with respect to the cylinder body 287. The cylinder body 287 is rotatably installed in the housing 211 and provides a driving force. The cylinder body 287 linearly drives the operating portion 289 by hydraulic or pneumatic pressure.

The operation unit 289 is installed in the cylinder body 287 so as to be reciprocated, and adjusts the striking member 284 to be rotatable with respect to the striking body 283. To this end, the striking body 283 is provided with a second pivot pin 283b spaced apart from the first pivot pin 283a, and an end of the operating portion 289 is coupled to the second pivot pin 283b. do.

As described above, by configuring the grinding unit 200, the mode switching unit 285 is controlled by the operation of the operation of the operation panel 700, so that the mode can be automatically switched.

FIG. 12 is a view showing the arrangement relationship between the striking unit 281 and the mode switching unit 285 in the spaced apart position, and FIG. 13 shows the arrangement relation of the striking unit 281 and the mode switching unit 285 in the striking position. The figure shown.

The striking member 284 rotatably installed with respect to the striking body 283 rotates with the first pivot pin 283a as the center of rotation according to the position of the second pivot pin 283b according to the moving distance of the operation unit 289. do.

When positioned as shown in FIG. 12 by the operation of the mode switching unit 285 (retraction of the operating unit 289), the striking member 284 is positioned at a spaced position in cooperation with the position of the operating unit 289. FIG. . When operating the grinding unit 200 in such a state serves as a centrifuge.

On the other hand, as shown in FIG. 13, when the operation unit 289 is pulled out, the second pivot pin 283b is moved clockwise as compared with the position shown in FIG. 12. Accordingly, the striking member 284 is rotated based on the first pivoting pin 283a, and the mode is switched from the spaced position to the striking position. When the grinding unit 200 operates in this state, the striking member 284 hits the grinding tube 10, thereby performing a homogenizer function.

Figure 14 is a schematic perspective view showing the main portion of the grinding unit according to a fourth embodiment of the cell treatment application system according to the present invention.

Referring to FIG. 14, the grinding unit 200 according to the fourth embodiment changes the striking unit 291 and the mode switching unit 295 provided in the housing 212, according to the third exemplary embodiment. The structure, function and arrangement of the other components, which are distinguished from the other components, are substantially identical. Therefore, in the detailed description of the present embodiment, the hitting unit 291 and the mode switching unit 295 will be described.

The striking portion 291 is operable between a striking position (see FIG. 16) that strikes the milling tube 10 when the holder 225 rotates, and a spaced position (see FIG. 15) spaced apart from the milling tube 10. It is installed in the housing 212.

The striking portion 291 includes a striking body 293 and a striking member 294 rotatably provided to the striking body 293. To this end, the striking body 293 is provided with a first pivot pin 293a coupled through the striking member 294. In addition, in order to operate the striking unit 291 in conjunction with the sliding movement of the slider 296, which will be described later, the striking body 293 is a second pivot provided at a position spaced apart from the first pivot pin 293a by a predetermined distance. Pin 293c may be provided. Here, a first guide long hole 293b for guiding the movement of the second pivoting pin 293c is formed in the striking body 293. Accordingly, the second pivot pin 293c moves along the first guide long hole 293b while receiving the force in one direction by the external force provided by the mode switching unit 295, and the striking member 294 moves the first pivot pin ( Rotate around 293a).

The mode switching unit 295 switches the mode so that the striking unit 294 is variable in position between the striking position and the spaced position. To this end, the mode switching unit 295 includes a slider 296 installed on the hitting body 293 so as to be slidable, and a driving unit 297 providing a driving force to the slider 296.

The slider 296 is slidably installed on the striking body 293 when power is supplied from the driving unit 297, and adjusts the striking member 294 to be rotatable with respect to the striking body 293. Here, the second guide long hole 296a to which the second pivot pin 293c is coupled is formed in the slider 296. Therefore, the movement of the second pivot pin 293c to the first and second guide holes 293b and 296b is two-dimensionally restricted, so that the striking member 294 is slid during the reciprocating sliding movement of the slide duct 296. It is rotated about the first pivot pin 293a.

The drive unit 297 includes a slide drive source 298 installed on the striking main body, and a power transfer unit 299 converting the rotational motion provided from the slide drive source 298 into a linear reciprocating motion and providing the slider 296 to the slider 296. .

The slide drive source 298 may be implemented as a drive motor that provides a rotational force. The power transmission unit 299 may include a worm gear 299a provided on the rotation shaft of the slide driving source 298 and a rack gear 299b formed at a predetermined position of the slide 296 that can be engaged with the worm gear 299a. Accordingly, the rack 296b engaged with the worm gear 299a linearly moves in a linear reciprocating motion according to the forward and reverse rotational motion of the slide drive source 298. Here, the slide drive source 298 can be controlled by the operation of the operation panel 700.

15 and 16 are views showing the arrangement relationship between the striking unit and the mode switching unit in each of the separation position and the striking position of the grinding unit according to the fourth embodiment of the cell treatment application system according to the present invention.

The striking member 294 rotatably installed with respect to the striking body 293 is rotated with the first pivot pin 293a as the center of rotation according to the position of the second pivot pin 293c according to the movement distance of the slider 296. do.

When positioned as shown in FIG. 15 by the operation of the mode switching unit 295, the striking member 294 is linked to the position of the slider 296 and positioned at a spaced position.

On the other hand, when the slider 296 is positioned as shown in FIG. 16 by the operation of the slide driving source 298, the second pivot pin 293c is moved clockwise as compared to the position shown in FIG. 15. Accordingly, the striking member 294 is rotated based on the first pivoting pin 293a, and the mode is switched from the spaced position to the striking position.

As described above, the grinding unit according to the first to fourth embodiments of the cell treatment application system according to the present invention adjusts the striking portion that strikes the grinding tube containing the target tissue between the striking position and the spaced position. Accordingly, there is an advantage that the homogenizer for separating the cells by the impact method and the centrifuge function for separating the cells by the centrifugation method can be performed.

Therefore, the application range of the cell treatment application system and the cell harvesting unit, the grinding unit and the spray unit therefor can be widely applied to the field of extracting stem cells from fat cells as well as burn treatment.

As described above, the tissue crushed product in the pulverizing tube 10 pulverized through the pulverizing unit 200 is filtered through the obtaining unit 300 to be described later to obtain a skin cell group 25. In addition, a portion of the skin cell group 25 obtained from the tissue debris is examined through the test unit 500.

Hereinafter, the test unit of the cell treatment application system according to the present invention will be described in detail with reference to FIGS. 17 and 18.

Figure 17 is a schematic cross-sectional view showing a test unit of the cell treatment application system according to the present invention, Figure 18 is a schematic perspective view showing a microscope of the test unit.

Referring to FIG. 17, the test unit 500 is provided in the test unit accommodating part 150a provided in the test unit mounting part 150. The inspection unit 500 includes a conveying unit 510 for transporting the inspected object 501, a microscope 550 for enlarging the tissue of the transported inspected object 501, and an enlarged inspected object 50. And a display unit 560 for displaying tissue of the. The object to be inspected 501 is composed of two inspection slides in which the skin cell group to be examined is accommodated. Here, the door 590 is provided in the opening of the test | inspection unit accommodation part 150a.

The transfer unit 510 transfers the object to be inspected 501 between a mounting position and an inspection position. To this end, the transfer unit 510 is a stage 520 which is installed to be horizontally movable with respect to the inspection unit mounting unit 150, and a lifting unit 530 provided to be able to be lifted and lowered by a predetermined driving source in a vertical direction with respect to the stage 520. And provided on the lifting unit 530 and includes an object stage 540 is mounted to the inspection object 501 on the upper surface.

The stage 520 is provided to be movable in the horizontal direction along the guide rail 511 provided in the inspection unit mounting unit 150. Here, as shown in FIG. 18, the stage 520 may move horizontally by transmitting the rotational force provided from the stage driving source 513 through the pinion 515 and the rack 517. On the other hand, the movement of the stage 520 is not limited to the configuration shown in Figure 18, various modifications are possible, such as a method using a linear motor.

The microscope 550 enlarges the tissue of the object to be inspected 501 transferred to the test position through the transfer unit 510 to a size that can be visually identified.

The display unit 560 is a device for displaying the tissue of the object to be inspected 50 enlarged by the microscope 550, and as shown in FIG. 17, the display unit 560 may be provided to move up and down the test unit mounting unit 150. To this end, the display unit 560 includes a display unit elevating guide unit 571, a display unit body 561 provided to be able to elevate the display unit elevating guide unit 571, and a display unit elevating driving unit for elevating and driving the display unit main body 561. 580. The display unit lifting and lowering unit 580 is a lifting belt 575 rotatably provided on a pair of pulleys 573 and 574 spaced apart from the lifting guide unit 571, and a lifting hole for reciprocating the lifting belt 575. Mobilization 581 is included. A power transmission belt 585 is included between the elevating driving unit 581 and the elevating belt 575 to transmit the power of the elevating driving unit 581 to the elevating belt 575. The display unit main body 561 has one position 563 fixed to the elevating belt 575, and is moved up and down in conjunction with the rotation of the elevating belt 575.

Here, although FIG. 17 illustrates a configuration in which the display unit 560 can be lifted and lowered by an electric method, the present invention is not limited thereto and various modifications are possible. That is, the display unit 560 may be configured to be fixed to the outside of the test unit mounting unit 150, or may be configured to be positioned on the test unit mounting unit 150 by a manual method.

When the inspection unit 500 is configured as described above, the operation panel 700 is opened while the door 590 is opened and the inspection object 501 is positioned on the stage 540 positioned at the mounting position. By operating, the lift unit 530, the stage 520, and the display unit 560 are drive controlled. Accordingly, the test object 501 may be moved to the test position, and the display unit 560 may protrude to the outside of the test unit mounting unit 150.

The obtaining unit 300 obtains a skin cell group (21 in FIG. 19) from the tissue crushed in the grinding unit 200. 19 to 27, an embodiment of the obtaining unit of the cell treatment application system according to the present invention will be described in detail.

19 is a schematic cross-sectional view showing a grinding tube after the grinding process of the cell treatment application system according to the embodiment of the present invention.

The grinding tube 10 is provided with a grinding target tissue (20 in FIG. 1) together with the grinding beads 30, and is provided to the grinding unit 200 with the opening closed through the grinding tube cap 15.

Looking at the grinding tube 10 after the grinding process, as shown in Figure 19, the target tissue is accommodated in the grinding tube 10 in a state of being crushed with the tissue crushed object (21). This tissue lysate 21 includes a skin cell group 25. Here, the skin cell group 25 includes at least one of keratinocytes, melanocytes, and fibrous cells, and is obtained through the obtaining unit 300.

20 is a schematic cross-sectional view showing an obtaining unit according to a first embodiment of a cell treatment application system according to the present invention, and FIG. 21 is a harvesting unit and a grinding tube according to a first embodiment of a cell treatment application system according to the present invention. This is an exploded perspective view showing the coupling relationship of.

19 to 21, the obtaining unit 300 according to the first embodiment of the cell treatment application system according to the present invention filters the skin cell group 25 from the tissue debris 21 contained in the grinding tube 10. The filter unit 310, and the spray tube 320 for receiving the filtered skin cell group (25).

The filter part 310 is a coupling member 311 which is lockably coupled to the coupling part 13 of the grinding tube 10, and a filter member which is installed in the opening of the grinding tube 10 by the coupling member 311 ( 315). Here, the grinding tube 10 and the coupling member 311 further includes a locking member (17, 313) provided in a shape corresponding to each other facing each other.

FIG. 21 shows an example in which a locking protrusion 17 is provided in the grinding tube 10 as a locking member and a locking groove 313 is formed in the coupling member 311. Here, the locking protrusion 17 has a structure in which the upper surface thereof is tapered in one direction, and in the screwing the coupling member 311 with the locking groove 313 formed in the grinding tube 10 in a predetermined direction, the coupling At the time when the member 311 is coupled to the grinding tube 10, the locking protrusion 17 is inserted into the locking groove 313 on the inclined surface thereof and is locked to each other. In this state, when rotating in the direction of releasing the spray tube 320 with respect to the grinding tube 10, the coupling member 311 maintains a locked state. Therefore, after the filter unit 310 filters the skin cell group 25 and separates the pulverized tube 10 and the spray tube 320, the coupling member 311 and the filter member 315 are sprayed from the spray tube 320. It may be separated to be located on the grinding tube 10 side.

In the present embodiment, the locking projection 17 is provided in the grinding tube 10, and the locking groove 313 is formed in the coupling member 311 is shown as an example, but the configuration of the locking member is not limited thereto. Various modifications are possible. For example, the locking groove is formed in the grinding tube 10, the locking protrusion is formed on the coupling member 311, or the locking protrusion is formed on both sides of the grinding tube 10 and the coupling member 311. Do.

Referring to FIG. 20, the spray tube 320 may further include a first protective cap 317. The first protective cap 317 is detachably mounted to the spray tube 320 so as to cover the opening of the filter unit 310 and the spray tube 320. By further including the first protective cap 317, it is possible to prevent foreign substances from flowing into the inner receiving space 323 of the spray tube 320, and contamination of the filter unit 310.

The spray tube 320 includes a spray tube body 321, a piston 327, and a second protective cap 350. The spray tube body 321 has an inner receiving space 323 for accommodating the skin cell group 25 and a spray hole 325 for spraying the skin cell group 25. The spray tube body 321 initially maintains a state in which the filter unit 310 and the first protective cap 317 are fastened to the opening, as shown in FIG. 20. On the other hand, the spray tube body 321 in a state that accommodates the skin cell group 25 therein, the first protective cap 317 and the filter unit 310 is removed.

The second protective cap 350 is detachably installed in the opening of the spray tube body 321, as shown in FIG. 24, to cover the opening of the spray tube 320 in the above state.

The piston part 327 is provided to be reciprocated in the receiving space 323 of the spray tube body 321, it is pressurized by the pressing portion 415 of the spray gun (410 of Figure 29) to be described later ( 323, the skin cell group 25 accommodated therein is pressed in the direction of the nozzle while entering the inside.

22 to 24 are each a view for explaining the coupling and separation process of the harvesting unit and the grinding tube according to the first embodiment of the cell treatment application system according to the present invention.

Referring to FIG. 22, the coupling member 311 and the filter member 315 are mounted in the opening of the spray tube 320, and are coupled to the grinding tube 10. At this time, through the locking members 17 and 313 provided in each of the grinding tube 10 and the coupling member 311, the coupling member 311 and the filter member 315 are locked to the grinding tube 10.

FIG. 23 illustrates that in the combined state of FIG. 22, the skin cell group 25 of the beads 30, the tissue debris 21, and the skin cell group 25 accommodated in the grinding tube 10 penetrates the filter member 315 to spray the tube. By moving to the receiving space 323 of 320, it shows a process of obtaining a skin cell group (25).

As such, when the grinding tube 10 is removed in the state of obtaining the skin cell group 25, the filter member 315 and the coupling member 311 are separated together with the grinding tube 10, and the spray tube 320 is Keep open. In this case, as shown in Figure 24 by coupling the second protective cap 350 to the opening of the spray tube 320, the acquisition of the skin cell group 25 is completed.

25 and 26 are schematic views for explaining the spraying operation in a state in which the nozzle and the obtaining unit according to the first embodiment of the cell treatment application system according to the present invention are combined.

Referring to FIG. 25, the nozzle 450 includes a nozzle body 451 and an injection tube 453 protruding in an awl shape at an end of the nozzle body 451. The injection tube 453 penetrates through the second protective cap 350 and is inserted into the spray tube 320. Here, the spray tube 320 is formed with a narrow neck spray opening than the receiving space 323, the end of the injection pipe 453 is inserted into the spray hole. Here, the skin cell group 25 inside the accommodation space 323 may further include a gasket 329 installed in the spray hole to prevent the skin cell group 25 from leaking through the space between the spray hole and the injection tube 453. .

Referring to FIG. 26, when the injection pipe 453 of the nozzle 450 is coupled to the spray tube 320, when the Pisonton part 327 is pressed into the receiving space 323 by the pressing part 415. The skin cell group 25 accommodated in the accommodation space 323 enters the nozzle direction and is sprayed through the nozzle.

As described above, by constituting the obtaining unit, the skin cell group is obtained from the tissue debris contained in the grinding tube in the state of being pulverized in the grinding unit and prevented from being exposed to the skin cell group in a series of processes mounted on the spray unit. Accordingly, the obtained skin cell group can be prevented from being contaminated by the external environment.

Figure 27 is a schematic separation perspective view showing the main portion of the harvesting unit according to the second embodiment of the cell treatment application system according to the present invention, Figure 28 is a schematic view showing the filter unit of the harvesting unit of Figure 27 coupled to the grinding tube It is a cross-sectional view.

The obtaining unit according to the second embodiment of the cell treatment application system according to the present invention is characterized in that the configuration of the filter unit 330 is changed in comparison with the obtaining unit according to the first embodiment. The components are substantially the same as the obtaining unit according to the first embodiment.

27 and 28, the filter unit 330 of the obtaining unit according to the second embodiment includes a coupling member 331 coupled to the grinding tube 10, and a grinding tube 10 by the coupling member 331. Filter member 335 and the first protective cap 337 is installed in the opening of the). The first protective cap 337 is installed on the coupling member 331 to cover the coupling member 331 and the filter member 335, and is separated from the coupling member 331 by an external force.

To this end, the filter unit 330 connects the first protective cap 337 and the coupling member 331, and further includes a plurality of bridges 339 cut by external force. The plurality of bridges 339 are provided between the first protective cap 337 and the coupling member 331. The bridges 339 are cut when a predetermined force is applied while the filter unit 330 is fastened to the grinding tube 10. 1 protective cap 337 is to be separated from the coupling member (331).

Here, the filter part 330 may be manufactured by insert injection molding so that the first protective cap 337, the filter member 335, the coupling member 331, and the bridge 339 are integrally injection molded.

29 is a schematic view showing a spray unit according to an embodiment of the cell treatment application system according to the present invention.

Referring to FIG. 29, the spray unit 400 according to an embodiment of the cell treatment application system according to the present invention includes a spray gun 410 and a nozzle 450.

The spray gun 410 accommodates the spray tube 320 with the second protective cap 350 installed on the spray tube body 321. The spray gun 410 pressurizes the piston portion 327 of the spray tube 320 so that the skin cell group accommodated in the spray tube 320 is sprayed in the direction of the nozzle 450.

The spray gun 410 is a pressurizing portion for pressing the piston portion 327 so that the trigger 411, the loading portion 413 for loading the spray tube 320 and the skin cell group accommodated in the spray tube 320 is injected 415. Here, the spray tube 320 is accommodated in the loading chamber of the spray gun 410 in a state in which the cover member 411 is opened, and by advancing the loading unit 413, the spray tube body 321 is directed in the nozzle direction. Proceed to At this time, the injection tube (453 of FIG. 26) of the nozzle 450 is inserted into the spray tube through the second protective cap 350 of the spray tube 320.

The pressurizing part 415 is operated in conjunction with the operation of the trigger 411, and pressurizes the piston part 327 into the receiving space of the spray tube 320 by pneumatic or hydraulic pressure. As a result, the skin cell group in the spray tube 320 is injected in the direction of the nozzle 450 at a predetermined pressure. Here, the spray gun 410 supplies power to the pressurizing unit 415 and may include a power supply unit 421 embedded in the spray gun 410. The power supply unit 421 may be configured as a primary battery or a rechargeable secondary battery. In addition, the spray gun 410 may be connected to an external power source by the adapter jack 425.

In addition, the spray gun 410 may further include an injection mode adjusting unit 430 for adjusting the injection operation. Through this injection mode adjusting unit 430, it is possible to control the continuous injection or intermittent injection, and the like. The spray gun 410 may further include a scattering preventing member 440 installed behind the nozzle 450 to prevent the sprayed skin cell group from scattering toward the user.

As described above, by providing the spray gun 410, there is an advantage that the skin cell group can be sprayed quickly and evenly to the injection target site.

30 to 32 are each a view for explaining the operating state of the nozzle of the spray unit according to an embodiment of the cell treatment application system according to the present invention.

Referring to FIG. 30, the nozzle 450 passes through a second protective cap (350 in FIG. 26) and has a nozzle body 451 having an injection tube 453 coupled to an injection hole of a spray tube, and is supplied from a spray tube. And a spraying unit 460 for spraying the skin cell group and an adjusting unit 470 for adjusting the spraying amount from the spraying unit 460. The spraying unit 460 elastically biases the opening and closing portions 461 and 462 opened by the pressure of the skin cell group sprayed through the spray gun 410 and elastically biases the closing portions 461 and 462 in the closing direction. ) And a spray tube 465. Here, the end of the spray pipe 465 has a structure that can be expanded according to the pressing force of the control unit 470.

The adjusting unit 470 is screwed to the nozzle body 451, so that the end of the spray pipe 465 is expanded according to the coupling position of the adjusting unit 470.

31 and 32 respectively show the spraying process of the nozzle 450, the diffusion angle of the skin cell group to be sprayed according to the control state of the control unit 470 is adjusted. That is, when adjusting the adjusting unit 470 in the state shown in FIG. 31 in the state shown in FIG. 32, the spray diffusion angle is increased as the end of the spray tube 465 is expanded.

The above embodiments are merely exemplary, and various modifications and equivalent other embodiments are possible to those skilled in the art. Therefore, the true technical protection scope of the present invention will be defined by the technical spirit of the invention described in the claims below.

Claims (45)

1. In the cell treatment application system for the treatment of skin defects,

   A main body;

   A pulverizing unit provided in the main body and pulverizing a predetermined target tissue accommodated in a pulverization tube together with a pulverizing bead;

   A obtaining unit for obtaining a skin cell group from tissue crushed in the grinding unit;

   Cell processing application system is provided in the main body, comprising a spray unit for spraying the skin cell group obtained in the obtaining unit to the cell treatment site.
2. The method of claim 1,

   The grinding unit includes a housing installed in the main body, a cover member covering the housing, a grinding drive source installed in the housing and providing rotational force, and a rotation shaft of the grinding drive source, wherein the grinding tube is mounted. And a striking portion installed in the housing to be operable between the holder, a striking position striking the milling tube when the holder is rotated, and a spaced position spaced apart from the milling tube, and the striking unit is the striking position and the Cell processing application system comprising a mode switching unit for switching the mode so that the position is variable between the separation position.
3. The method of claim 2,

   The striking unit includes a striking body and at least one striking member rotatably installed with respect to the striking body,

   The mode switching unit may be installed to be capable of a relative reciprocating rotational movement with respect to the housing, and includes a mode switching body having a mode switching hole through which a portion of the striking member is inserted, and an operation unit for reciprocating the mode switching body. Cell processing application system characterized in that.
4. The method of claim 2,

   The striking unit includes a striking body and at least one striking member rotatably installed with respect to the striking body,

   The mode switching unit is installed in the housing, the cylinder body for providing a driving force and the reciprocating movement with respect to the cylinder body, including an operation unit for adjusting the striking member to be rotatable relative to the striking body Cell processing application system characterized in that.
5. The method of claim 2,

   The striking unit includes a striking body and at least one striking member rotatably installed with respect to the striking body,

   The mode switching unit includes a slider which is slidably mounted to the hitting body, the slider which adjusts the hitting member to be rotatable with respect to the hitting body, and a driving unit which provides driving force to the slider. Applicable system.
6. The method of claim 5,

   The driving unit includes a slide driving source installed on the hitting body and a power transmission unit for converting the rotational movement provided from the slide driving source to a linear reciprocating motion to provide to the slider.
7. The method according to any one of claims 2 to 6,

   The grinding unit, the cell processing application system further comprises a clamp for clamping the grinding tube mounted to the holder.
8. The method of claim 7, wherein

   The clamp includes a clamp main body which prevents the grinding tube mounted on the grinding tube mounting groove of the holder from being separated during operation, and a fixing cap which fixes the clamp main body to the rotation shaft of the grinding driving source. Processing application system.
9. The method of claim 7, wherein

   The clamp may include a guide shaft fixed to the inside of the cover member and a guide shaft which is fixed to the guide shaft so as to be lifted and rotated so that the grinding tube mounted in the grinding tube mounting groove of the holder is not separated during operation. An elastic member interposed between the main body, the cover member, and the clamp main body to elastically bias the clamp main body in the pressing direction of the grinding tube, and a rotation support part supporting the clamp main body to freely rotate with respect to the cover member. Cell processing application system comprising a.
10. The method of claim 1,

    The obtaining unit is a cell treatment application system comprising a filter unit for filtering the skin cell group from the tissue debris contained in the grinding tube, and a spray tube for receiving the skin cell group filtered by the filter unit.
11. The method of claim 10,

    And the filter unit includes a coupling member lockably coupled to the grinding tube, and a filter member installed in the opening of the grinding tube by the coupling member.
12. The method of claim 11,

    A locking member provided in a shape corresponding to each other of the crushing tube and the opposite surface of the coupling member is further included,

    After the filter unit filters the skin cell group and separates the pulverization tube and the spray tube, the coupling member and the filter member are separated from the spray tube so as to be positioned on the pulverization tube side. .
13. The method of claim 11,

    The spray tube,

    And a first protective cap detachably installed on the coupling member so as to cover the filter unit and protecting the spray tube.
14. The method of claim 10,

    The filter unit, the coupling member coupled to the grinding tube, the filter member is installed in the opening of the grinding tube by the coupling member, the coupling member is installed on the coupling member to cover the coupling member and the filter member, the external force Cell treatment application system comprising a first protective cap detachable from the coupling member by.
15. The method of claim 14,

    The first protective cap and the coupling member, a plurality of bridges are cut by an external force is provided between the spray tube protective cap and the coupling member, characterized in that the cell treatment application system.
16. The method according to any one of claims 10 to 15,

    The spray tube has an inner receiving space for accommodating the skin cell group and a spray hole for spraying the skin cell group, the spray tube body which can be coupled to the coupling member, and detachable to the spray tube body to cover the spray hole. Cell protection application system comprising a second protective cap that is possibly installed, and a piston unit reciprocally provided in the receiving space of the spray tube body.
17. The method of claim 16,

    The spray tube, the cell treatment application system further comprises a gasket installed in the spray hole.
18. The method of claim 16,

    The spraying unit includes a spray gun for accommodating the spray tube in a state where the second protective cap is installed on the spray tube body, and pressurizing the piston to spray a skin cell group accommodated in the spray tube, and to the spray gun. To be installed, the cell treatment application system comprising a nozzle for spraying the skin cell group sprayed from the spray tube to the affected part.
19. The method of claim 18,

    The spray gun includes a trigger, a loading portion for loading the spray tube, and a pressurizing portion for pressurizing the piston portion so as to spray the skin cell group accommodated in the spray tube in conjunction with the operation of the trigger. Cell treatment application system.
20. The method of claim 19,

    The spray gun supplies power to the pressurization unit, and further includes a power supply unit embedded in the spray gun.
21. The method of claim 18,

    The nozzle may include a nozzle body having an injection tube coupled to the spray hole of the spray tube through the second protective cap, a spray unit spraying the skin cell group supplied from the spray tube, and from the spray unit. Cell processing application system comprising a control unit for adjusting the injection amount of.
22. The method according to any one of claims 1 to 6 and 10 to 15,

    The cell treatment application system provided in the main body, further comprising a test unit for testing the skin cell group obtained from the tissue crushed in the grinding unit.
23. The method of claim 22,

    The inspection unit includes a transfer unit for transporting the inspected object, a microscope for enlarging the tissue to be inspected, and a display unit installed in the main body and displaying the enlarged tissue of the inspected object. Cell processing application system.
24. The method of claim 23, wherein

    The transfer unit is provided on a stage provided to be movable horizontally with respect to the inspection unit mounting unit provided in the main body, a lifting unit provided to be liftable by a predetermined driving source in a direction perpendicular to the stage, and the lifting unit, Cell treatment application system comprising a stage on which the test object is mounted on the upper surface.
25. The method according to any one of claims 1 to 6 and 10 to 15,

    The skin cell group, cell treatment application system comprising at least one of keratinocytes, melanocytes and fibroblasts.
26. In the cell harvesting unit for the treatment of skin defects,

    A grinding tube in which the ground tissue debris is accommodated;

    A filter unit for filtering a predetermined cell group from the tissue debris contained in the grinding tube;

    And a spray tube having an inner receiving space accommodating the cell group filtered by the filter unit and a spray hole for spraying the cell group.
27. The method of claim 26,

    The filter unit, the cell harvesting unit, characterized in that it comprises a coupling member lockably coupled to the grinding tube, and the filter member is installed in the opening of the grinding tube by the coupling member.
28. The method of claim 27,

    A locking member provided in a shape corresponding to each other of the crushing tube and the opposite surface of the coupling member is further included,

    When the filter unit is filtering the cell group, when separating the grinding tube and the spray tube, the coupling member and the filter member is separated from the spray tube is characterized in that the cell harvesting unit is located on the grinding tube side.
29. The method of claim 27, wherein the spray tube,

    Cell covering unit further comprises a first protective cap to be detachably installed on the coupling member to cover the filter unit to protect the spray tube.
30. The method of claim 26,

    The filter unit, the coupling member coupled to the grinding tube, the filter member is installed in the opening of the grinding tube by the coupling member, the coupling member is installed on the coupling member to cover the coupling member and the filter member, the external force Cell obtaining unit, characterized in that it comprises a first protective cap that can be separated from the coupling member.
31. The method of claim 30,

    A cell obtaining unit connecting the first protective cap and the coupling member, wherein a plurality of bridges cut by an external force are provided between the spray tube protective cap and the coupling member.
32. The method of any one of claims 26 to 31,

    The spray tube has an inner receiving space for accommodating the cell group and a spray hole for spraying the cell group, the spray tube body which can be coupled to the coupling member, and the spray tube body so as to cover the spray hole, detachably attached to the spray tube body. Cell harvesting unit, characterized in that it comprises a second protective cap that is installed, and a piston portion reciprocally provided in the receiving space of the spray tube body.
33. 33. The method of claim 32,

    The spray tube, the cell harvesting unit further comprises a gasket installed in the spray hole.
34. In the tissue grinding unit for the treatment of skin defects,

    A housing;

    A cover member covering the housing;

    A grinding drive source installed in the housing and providing rotational force;

    A holder installed on the rotating shaft of the grinding drive source and equipped with a grinding tube for receiving the crushed tissue crushed material;

    A striking part installed in the housing to be operable between a striking position striking the milling tube and a spaced position spaced apart from the milling tube when the holder is rotated;

    Tissue grinding unit characterized in that it comprises a mode switching unit for switching the mode so that the striking portion is variable between the striking position and the spaced position.
35. The method of claim 34, wherein

    The striking unit includes a striking body and at least one striking member rotatably installed with respect to the striking body,

    The mode switching unit may be installed to be capable of a relative reciprocating rotational movement with respect to the housing, and includes a mode switching body having a mode switching hole through which a portion of the striking member is inserted, and an operation unit for reciprocating the mode switching body. Tissue grinding unit characterized in that.
36. The method of claim 34, wherein

    The striking unit includes a striking body and at least one striking member rotatably installed with respect to the striking body,

    The mode switching unit, the cylinder body is provided in the housing and provides a driving force; Tissue crushing unit characterized in that it is installed so as to reciprocate with respect to the cylinder body, the operation unit for adjusting the striking member to be rotatable relative to the striking body.
37. The method of claim 34, wherein

    The striking unit includes a striking body and at least one striking member rotatably installed with respect to the striking body,

    The mode switching unit is provided with a sliding movement to the striking body, and a slider for adjusting the striking member to be rotatable relative to the striking body, and tissue grinding, characterized in that the drive unit for providing a driving force to the slider unit.
38. The method of claim 37,

    The driving unit includes a slide driving source installed on the hitting body, and a power transmission unit for converting the rotational movement provided from the slide driving source to a linear reciprocating motion to provide to the slider.
39. The method according to any one of claims 34 to 38,

    The grinding unit further comprises a clamp for clamping the grinding tube mounted to the holder.
40. The method of claim 39,

    The clamp includes a clamp main body which prevents the grinding tube mounted on the grinding tube mounting groove of the holder from being separated during operation, and a fixing cap which fixes the clamp main body to the rotating shaft of the grinding driving source. Grinding unit.
41. The method of claim 39,

    The clamp may include a guide shaft fixed to the inside of the cover member and a guide shaft which is fixed to the guide shaft so as to be lifted and rotated so that the grinding tube mounted in the grinding tube mounting groove of the holder is not separated during operation. An elastic member interposed between the main body, the cover member, and the clamp main body to elastically bias the clamp main body in the pressing direction of the grinding tube, and a rotation support part supporting the clamp main body to freely rotate with respect to the cover member. Tissue grinding unit comprising a.
42. In the cell spray unit for the treatment of skin defects,

    A spray tube body having an inner receiving space accommodating the cell group and a spray hole for spraying the cell group;

    A protective cap detachably installed on the spray tube body to cover the spray hole;

    A spray tube including a piston unit provided in the accommodation space of the spray tube body to be reciprocated;

    A spray gun configured to receive the spray tube and pressurize the piston to inject a cell group in the inner accommodating space of the spray tube;

    Cell spray unit, characterized in that installed in the spray gun, comprising a nozzle for spraying the cell group injected from the spray tube to the affected part.
43. The method of claim 42, wherein

    The spray gun includes a trigger, a loading portion for loading the spray tube, and a pressurizing portion for pressurizing the piston portion so that the cell group accommodated in the spray tube is interlocked with the operation of the trigger. Spray unit.
44. The method of claim 43,

    The spray gun is to supply power to the pressurization unit, the cell spray unit, characterized in that it further comprises a power supply unit embedded in the spray gun.
45. The method of any one of claims 42-44,

    The nozzle, the nozzle body having an injection tube coupled to the injection port of the spray tube through the protective cap, the spray unit for spraying the cell group supplied from the spray tube, and the injection amount from the spray unit Cell spray unit comprising a control unit for controlling.

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